KR850002285A - 면역 인터페론의 제조방법 - Google Patents

면역 인터페론의 제조방법 Download PDF

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KR850002285A
KR850002285A KR1019840005714A KR840005714A KR850002285A KR 850002285 A KR850002285 A KR 850002285A KR 1019840005714 A KR1019840005714 A KR 1019840005714A KR 840005714 A KR840005714 A KR 840005714A KR 850002285 A KR850002285 A KR 850002285A
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ser
asp
asn
phe
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KR1019840005714A
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KR940004544B1 (en
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수스무(외 2) 혼다
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원본미기재
에프. 호프만 라 롯슈 앤드캄파니 아크티엔 게젤샤프트(외 1)
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Application filed by 원본미기재, 에프. 호프만 라 롯슈 앤드캄파니 아크티엔 게젤샤프트(외 1) filed Critical 원본미기재
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/555Interferons [IFN]
    • C07K14/57IFN-gamma
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract

내용 없음

Description

면역 인터페론의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제3도는 CNBr처리후, 15K 및 18K rIFN-г의 C-말단 펩티드를 HPLC분리이다. 제4도는 18K rIFN-г의 트립신 분해 펩티드지도이다.

Claims (10)

  1. 형질 전환된 미생물로 부터 단백질 분해 효소 억제제로 온전한 재조합 인체 면역 인터페론을 추출하고, 추출한 인터페론을 모노크로날 항체를 사용하여 정제함을 특징으로 하여, 인터페론을 함유하는 형질 전환된 미생물로 부터 본래 그의 단백질 분해 단편이 없는 하기의 아미노산 서열을 갖는 온전한 재조합 인체 면역 인터페론을 제조하는 방법.
  2. Cys Tyr Cys Gln Asp Pro Tyr Val Lys Glu Ala Glu Asn Leu Lys Tyr Phe Asn Ala Gly His Ser Asp Val Ala Asp Asn Gly Thr Leu Phe Leu Ile Leu Lys Asn Trp Lys Glu Glu Ser Asp ArgLys Ile Met Gln Ser Gln Ile Val Ser Phe Tyr Phe Lys Leu Phe Lys Asn Phe Lys Asp Asp Gln Ser Ile Gln Lys Ser Val Glu Thr Ile Lys Glu Asp Met Asn Val Lys Phe Phe Asn Ser Asn Lys Lys Lys Arg Asp Asp Phe Glu Lys Leu Thr Asn Tyr Ser Val Tyr Asp Leu Asn Val Gln Arg Lys Ala Ile His Glu Leu Ile Gln Vla Met Ala Glu Leu Ser Pro Ala All Lys Thr Gly Lys Arg Lys Arg Ser Gln Met Leu Phe Arg Gly Arg Gly Arg Arg Ala Ser Gln
  3. (상기 서열의 아미노 말단에 부가적인 메티오닌 잔기가 임의로 포함될 수 있다.)
  4. 제1항에 있어서, 단백질 분해 효소 억제제가 구아니디늄 염인 방법.
  5. 제1항에 있어서, 단백질 분해 효소 억제제가 구아니딘-HCl인 방법.
  6. 제2 또는 3항에 있어서 구아니디늄 염이 용액내에 존재하는 방법.
  7. 제1항에 있어서, 형질 전환된 미생물을 구아니딘-HCl의 용액에 현탁시키는 방법.
  8. 제5항에 있어서, 구아니딘-HCl용액의 농도가 최소한 약 4M인 방법.
  9. 제5 또는 6항에 있어서, 형질 전환된 미생물 g당 최소한 약 3배 용적의 구아니디늄의 용액을 사용하는 방법.
  10. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR84005714A 1983-09-20 1984-09-19 Immune interferon and method for its extraction and purification KR940004544B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53404083A 1983-09-20 1983-09-20
US534040 1983-09-20

Publications (2)

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KR850002285A true KR850002285A (ko) 1985-05-10
KR940004544B1 KR940004544B1 (en) 1994-05-25

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ID=24128473

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Application Number Title Priority Date Filing Date
KR84005714A KR940004544B1 (en) 1983-09-20 1984-09-19 Immune interferon and method for its extraction and purification

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EP (1) EP0138087B1 (ko)
JP (1) JP2592441B2 (ko)
KR (1) KR940004544B1 (ko)
AT (1) ATE33842T1 (ko)
AU (1) AU590712B2 (ko)
DE (1) DE3470747D1 (ko)
DK (1) DK162604C (ko)
ES (1) ES8606886A1 (ko)
FI (1) FI80293C (ko)
GB (1) GB2146998B (ko)
GR (1) GR80405B (ko)
HU (1) HU196461B (ko)
IL (1) IL72986A (ko)
MC (1) MC1627A1 (ko)
NO (1) NO161274C (ko)
NZ (1) NZ209588A (ko)
PH (1) PH23241A (ko)
PT (1) PT79232B (ko)
ZA (1) ZA846554B (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2164650A (en) * 1984-09-25 1986-03-26 Fujisawa Pharmaceutical Co Process for production of y-interferon
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
DE19755960C1 (de) 1997-12-16 1998-11-26 Hoechst Ag Verfahren zum Aufschluß von biologischem Material

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL7404589A (nl) * 1974-04-03 1975-10-07 Stichting Rega V Z W Werkwijze voor het stabiliseren van interferon.
DE3005897A1 (de) * 1980-02-16 1981-09-03 Hoechst Ag, 6000 Frankfurt Genprodukt eines hoeheren organismus aus einem dieses gen enhtaltenden mikroorganismus
IL65475A0 (en) * 1981-04-17 1982-07-30 Meloy Lab Production of immune interferon and its mrna
IL63327A (en) * 1981-07-16 1985-11-29 Yeda Res & Dev Production of interferon beta1 and alpha-phage recombinants for its production in host bacteria
AU561343B2 (en) * 1981-10-19 1987-05-07 Genentech Inc. Human immune interferon by recombinant dna
JPS58110548A (ja) * 1981-12-24 1983-07-01 Asahi Chem Ind Co Ltd 新規な生理活性ペプチド
EP0088540A3 (en) * 1982-02-22 1984-10-17 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing human immune interferon-like polypeptides
IL68100A0 (en) * 1982-03-24 1983-06-15 Takeda Chemical Industries Ltd Novel dna and use thereof
IL69851A0 (en) * 1982-09-30 1983-12-30 Japan Found Cancer Novel dna and recombinant plasmid containing the same
WO1984002129A1 (en) * 1982-11-22 1984-06-07 Takeda Chemical Industries Ltd Human immune interferon protein and process for its preparation
JPH0740925B2 (ja) * 1983-03-14 1995-05-10 協和醗酵工業株式会社 新規ヒトインタ−フエロン−γポリペプチド
US4855238A (en) * 1983-12-16 1989-08-08 Genentech, Inc. Recombinant gamma interferons having enhanced stability and methods therefor

Also Published As

Publication number Publication date
GB2146998B (en) 1987-10-07
GR80405B (en) 1985-01-21
FI80293C (fi) 1990-05-10
AU3330184A (en) 1985-03-28
EP0138087A1 (en) 1985-04-24
DK162604C (da) 1992-05-11
DK162604B (da) 1991-11-18
HU196461B (en) 1988-11-28
NO161274B (no) 1989-04-17
JPS6169800A (ja) 1986-04-10
PH23241A (en) 1989-06-06
GB2146998A (en) 1985-05-01
HUT35289A (en) 1985-06-28
FI843669L (fi) 1985-03-21
IL72986A (en) 1990-04-29
FI843669A0 (fi) 1984-09-19
PT79232A (en) 1984-10-01
KR940004544B1 (en) 1994-05-25
DK447784D0 (da) 1984-09-19
ES8606886A1 (es) 1986-05-16
DK447784A (da) 1985-03-21
PT79232B (en) 1986-11-18
NO161274C (no) 1989-07-26
JP2592441B2 (ja) 1997-03-19
AU590712B2 (en) 1989-11-16
NO843750L (no) 1985-03-21
DE3470747D1 (en) 1988-06-01
MC1627A1 (fr) 1985-09-26
ZA846554B (en) 1985-04-24
IL72986A0 (en) 1984-12-31
EP0138087B1 (en) 1988-04-27
NZ209588A (en) 1989-05-29
ATE33842T1 (de) 1988-05-15
FI80293B (fi) 1990-01-31
ES536028A0 (es) 1986-05-16
GB8423748D0 (en) 1984-10-24

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