KR830003898A - 인체 암 핵인 항원에 대한 항체를 사용한 인체 암세포의 검출 - Google Patents
인체 암 핵인 항원에 대한 항체를 사용한 인체 암세포의 검출 Download PDFInfo
- Publication number
- KR830003898A KR830003898A KR1019800005026A KR800005026A KR830003898A KR 830003898 A KR830003898 A KR 830003898A KR 1019800005026 A KR1019800005026 A KR 1019800005026A KR 800005026 A KR800005026 A KR 800005026A KR 830003898 A KR830003898 A KR 830003898A
- Authority
- KR
- South Korea
- Prior art keywords
- nucleus
- antigen
- antibody
- cells
- human cancer
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title claims 7
- 102000036639 antigens Human genes 0.000 title claims 7
- 108091007433 antigens Proteins 0.000 title claims 7
- 201000011510 cancer Diseases 0.000 title claims 7
- 206010028980 Neoplasm Diseases 0.000 title claims 5
- 238000001514 detection method Methods 0.000 title claims 2
- 210000004027 cell Anatomy 0.000 claims 4
- 241001465754 Metazoa Species 0.000 claims 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 2
- 239000007983 Tris buffer Substances 0.000 claims 2
- 239000000284 extract Substances 0.000 claims 2
- 239000011574 phosphorus Substances 0.000 claims 2
- 229910052698 phosphorus Inorganic materials 0.000 claims 2
- 241000894007 species Species 0.000 claims 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 2
- 210000004881 tumor cell Anatomy 0.000 claims 2
- 229920005654 Sephadex Polymers 0.000 claims 1
- 239000012507 Sephadex™ Substances 0.000 claims 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 claims 1
- 229910000389 calcium phosphate Inorganic materials 0.000 claims 1
- 239000001506 calcium phosphate Substances 0.000 claims 1
- 235000011010 calcium phosphates Nutrition 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 238000004440 column chromatography Methods 0.000 claims 1
- 230000007717 exclusion Effects 0.000 claims 1
- 238000005227 gel permeation chromatography Methods 0.000 claims 1
- 230000001900 immune effect Effects 0.000 claims 1
- 230000003053 immunization Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57496—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving intracellular compounds
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Detergent Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (4)
- 표본을 항체와 접촉시켜서 정상세포가 아닌 악성 종양세포의 핵인에서 상기 항체가 국소화함을 증명하는 것을 특징으로 하는 인체 악성 종양세포의 핵인 또는 이런 세포의 핵 또는 핵인 추출물에서 끄집어 낸 항체를 사용하는 인체 조직절편, 도말표본 및 탈락 세포검사용 표본중 암의 면역학적 검출방법.
- 비인간 숙주 동물을 항원으로 면역시키고 면역된 동물로부터 항체를 생성함을 특징으로 하여 항체를 암세포의 핵인 및 핵중에 존재하는 항원에 대해 특이성을 갖도록 제조하는 방법. 이때 면역시키는 항원은 1차 및 2차 종을 가지며 1차 종은 약 6.0 내지 0.7 등전점 및 약 50,000 내지 60,000 돌턴의 분자량을 가지며 pH8인 트리스 CH1에 용해하며 주로 핵인에 국재한다.
- 인체 암세포의 핵 또는 핵인으로부터 pH7 내지 9인 0.01M 트리스 HC1로 항원을 추출하여 핵인 항원을 제조 및 분리하는 방법.
- 황산 암모늄 침전, DEAE-칼럼 크로마토그라피, 세파덱스겔 배제, 양이온 교환 및 인산칼슘겔 크로마토그라피 및 등전점 조정등 일련의 정제를 포함하는 핵인 항원의 정제방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000343088A CA1157373A (en) | 1980-01-04 | 1980-01-04 | Detection of human cancer cells with antibodies to human cancer nucleolar antigen(s) |
| CA343,088 | 1980-01-04 | ||
| CA343088 | 1980-01-04 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR830003898A true KR830003898A (ko) | 1983-06-30 |
| KR850001770B1 KR850001770B1 (ko) | 1985-12-18 |
Family
ID=4115973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019800005026A KR850001770B1 (ko) | 1980-01-04 | 1980-12-30 | 인체 암 항원에 대한 특이항체의 제조방법 |
Country Status (27)
| Country | Link |
|---|---|
| JP (1) | JPS56101553A (ko) |
| KR (1) | KR850001770B1 (ko) |
| AR (1) | AR232046A1 (ko) |
| AT (1) | AT373397B (ko) |
| AU (1) | AU535024B2 (ko) |
| BE (1) | BE886935A (ko) |
| CA (1) | CA1157373A (ko) |
| CH (1) | CH659328A5 (ko) |
| DE (1) | DE3047654A1 (ko) |
| DK (1) | DK554980A (ko) |
| ES (2) | ES498305A0 (ko) |
| FI (1) | FI810007L (ko) |
| FR (1) | FR2484650B1 (ko) |
| GB (1) | GB2067286B (ko) |
| GR (1) | GR72850B (ko) |
| IE (1) | IE50760B1 (ko) |
| IL (1) | IL61641A (ko) |
| IT (1) | IT1172216B (ko) |
| LU (1) | LU83015A1 (ko) |
| NL (1) | NL8007128A (ko) |
| NO (2) | NO803910L (ko) |
| NZ (1) | NZ195756A (ko) |
| OA (1) | OA06713A (ko) |
| PH (5) | PH18765A (ko) |
| PT (1) | PT72301B (ko) |
| SE (1) | SE450665B (ko) |
| ZA (1) | ZA807754B (ko) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4487830A (en) * | 1982-05-14 | 1984-12-11 | American Hoechst Corporation | Enzyme/immunofluorescent assay for autoantibodies |
| JPS5990052A (ja) * | 1982-11-16 | 1984-05-24 | Katsu Taniguchi | モノクロ−ナル特異抗体を用いたメラノ−マ診断薬 |
| US4746539A (en) * | 1983-11-23 | 1988-05-24 | The Ohio State University Research Foundation | Purification of cancer-associated protein and preparation of antibody thereto |
| EP0145373B1 (en) * | 1983-11-23 | 1992-03-25 | The Ohio State University Research Foundation | Purification of cancer-associated protein and preparation of antibody thereto |
| US4871661A (en) * | 1983-11-23 | 1989-10-03 | The Ohio State University Research Foundation | Process for testing the carcinogenicity of a material or the presence of cancer-inducing factors in an environment |
| US4645737A (en) * | 1984-03-05 | 1987-02-24 | American Hoechst Corporation | Enzyme/immunofluorescent assay for anti-treponemal antibodies |
| US4607008A (en) * | 1984-03-05 | 1986-08-19 | American Hoechst Corporation | Enzyme/immunofluorescent assay for anti-Epstein-Barr Virus antibodies |
| EP0162977B1 (en) * | 1984-05-02 | 1992-05-20 | University Research Corporation | Enzyme immunoassay for cancer procoagulant |
| US4616658A (en) * | 1985-02-27 | 1986-10-14 | William Shell | Non-radioactively labeled microspheres and use of same to measure blood flow |
| US4798719A (en) * | 1986-09-11 | 1989-01-17 | University Of Pittsburgh | Method for selection of antigens suitable as in vivo targets for antibodies |
| US5019368A (en) * | 1989-02-23 | 1991-05-28 | Cancer Biologics, Inc. | Detection of necrotic malignant tissue and associated therapy |
| US4861581A (en) * | 1986-12-05 | 1989-08-29 | Cancer Biologics, Inc. | Detection of necrotic malignant tissue and associated therapy |
| CA2023030A1 (en) * | 1990-07-13 | 1992-01-14 | Robert R. Guerrero | In vitro method and probe for detecting the presence of the ring shaped particle and malignancy in humans and animals |
-
1980
- 1980-01-04 CA CA000343088A patent/CA1157373A/en not_active Expired
- 1980-12-04 IE IE2533/80A patent/IE50760B1/en unknown
- 1980-12-04 NZ NZ195756A patent/NZ195756A/xx unknown
- 1980-12-04 AU AU65070/80A patent/AU535024B2/en not_active Ceased
- 1980-12-05 IL IL61641A patent/IL61641A/xx unknown
- 1980-12-10 ZA ZA00807754A patent/ZA807754B/xx unknown
- 1980-12-15 PH PH24978A patent/PH18765A/en unknown
- 1980-12-17 DE DE19803047654 patent/DE3047654A1/de active Granted
- 1980-12-18 LU LU83015A patent/LU83015A1/fr unknown
- 1980-12-22 NO NO803910A patent/NO803910L/no unknown
- 1980-12-22 AR AR283753A patent/AR232046A1/es active
- 1980-12-27 JP JP18948880A patent/JPS56101553A/ja active Pending
- 1980-12-29 DK DK554980A patent/DK554980A/da not_active Application Discontinuation
- 1980-12-30 CH CH9652/80A patent/CH659328A5/de not_active IP Right Cessation
- 1980-12-30 KR KR1019800005026A patent/KR850001770B1/ko active
- 1980-12-31 GB GB8041638A patent/GB2067286B/en not_active Expired
- 1980-12-31 NL NL8007128A patent/NL8007128A/nl not_active Application Discontinuation
- 1980-12-31 PT PT72301A patent/PT72301B/pt unknown
- 1980-12-31 BE BE0/203378A patent/BE886935A/fr not_active IP Right Cessation
-
1981
- 1981-01-02 ES ES498305A patent/ES498305A0/es active Granted
- 1981-01-02 FR FR8100014A patent/FR2484650B1/fr not_active Expired
- 1981-01-02 FI FI810007A patent/FI810007L/fi not_active Application Discontinuation
- 1981-01-02 SE SE8100011A patent/SE450665B/sv not_active IP Right Cessation
- 1981-01-05 OA OA57289A patent/OA06713A/xx unknown
- 1981-01-05 AT AT0001081A patent/AT373397B/de not_active IP Right Cessation
- 1981-01-05 IT IT67001/81A patent/IT1172216B/it active
- 1981-01-05 GR GR63789A patent/GR72850B/el unknown
-
1982
- 1982-02-01 ES ES509239A patent/ES8307377A1/es not_active Expired
-
1983
- 1983-02-21 PH PH28548A patent/PH18624A/en unknown
- 1983-02-21 PH PH28547A patent/PH18637A/en unknown
- 1983-02-21 PH PH28549A patent/PH18636A/en unknown
- 1983-02-21 PH PH28550A patent/PH18129A/en unknown
-
1985
- 1985-01-02 NO NO850005A patent/NO850005L/no unknown
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