KR20240144409A - 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스 - Google Patents

단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스 Download PDF

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KR20240144409A
KR20240144409A KR1020247030391A KR20247030391A KR20240144409A KR 20240144409 A KR20240144409 A KR 20240144409A KR 1020247030391 A KR1020247030391 A KR 1020247030391A KR 20247030391 A KR20247030391 A KR 20247030391A KR 20240144409 A KR20240144409 A KR 20240144409A
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signal
cell
impedance value
detection
electrical signal
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Korean (ko)
Inventor
제프리 잔
밍데 젱
데이비드 아이. 슈라이버
하오 린
제리 더블유. 샨
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룻거스, 더 스테이트 유니버시티 오브 뉴저지
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Publication of KR20240144409A publication Critical patent/KR20240144409A/ko
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
KR1020247030391A 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스 Pending KR20240144409A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562214665P 2015-09-04 2015-09-04
US62/214,665 2015-09-04
KR1020187008870A KR20180042422A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스
PCT/US2016/050201 WO2017040995A1 (en) 2015-09-04 2016-09-02 High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells

Related Parent Applications (1)

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KR1020187008870A Division KR20180042422A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스

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Publication Number Publication Date
KR20240144409A true KR20240144409A (ko) 2024-10-02

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KR1020247030391A Pending KR20240144409A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스
KR1020187008870A Ceased KR20180042422A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스

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Country Status (9)

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US (2) US10927333B2 (enExample)
EP (1) EP3344746B1 (enExample)
JP (1) JP6989133B2 (enExample)
KR (2) KR20240144409A (enExample)
CN (1) CN109153956B (enExample)
AU (1) AU2016315883B2 (enExample)
BR (1) BR112018004278B1 (enExample)
CA (1) CA2997435C (enExample)
WO (1) WO2017040995A1 (enExample)

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CN109679844B (zh) * 2017-10-19 2023-08-22 苏州壹达生物科技有限公司 一种流式电穿孔装置
WO2019194799A1 (en) 2018-04-04 2019-10-10 Hewlett-Packard Development Company, L.P. Microfluidic cellular membrane modification devices
US20190338235A1 (en) * 2018-05-01 2019-11-07 The Charles Stark Draper Laboratory, Inc. Method and Device for Exosomes Electroporation
EP3820612A4 (en) * 2018-07-12 2022-05-04 GPB Scientific, Inc. MICROFLUID PROCESSES FOR CELL MANUFACTURE
WO2020041480A1 (en) * 2018-08-21 2020-02-27 The Regents Of The University Of California High-throughput system and method for the temporary permeabilization of cells using lipid bilayers
KR20210089640A (ko) * 2018-08-31 2021-07-16 더 차레스 스타크 드레이퍼 래보레이토리, 인코포레이티드 세포의 고처리량 고효율 형질 주입을 위한 방법 및 장치
JP2022509497A (ja) * 2018-10-26 2022-01-20 カイトペン コーポレイション 電気穿孔のためのデバイス、システム、及びキット、ならびにそれらの使用方法
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CA3124709C (en) * 2019-02-04 2025-04-08 Univ Rutgers DEVICE AND METHOD FOR ELECTROTRANSFERRING TISSUE USING A MICROELECTRODE
CA3138353A1 (en) 2019-05-16 2020-11-19 CyteQuest, Inc. Devices, methods, and systems for electroporation
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EP3344746A1 (en) 2018-07-11
JP6989133B2 (ja) 2022-01-05
US20180258379A1 (en) 2018-09-13
CA2997435A1 (en) 2017-03-09
CN109153956B (zh) 2022-10-28
AU2016315883B2 (en) 2021-01-28
AU2016315883A1 (en) 2018-03-29
KR20180042422A (ko) 2018-04-25
CA2997435C (en) 2022-11-08
US11987777B2 (en) 2024-05-21
EP3344746A4 (en) 2019-01-16
BR112018004278B1 (pt) 2022-05-03
BR112018004278A2 (enExample) 2018-10-02
JP2018529333A (ja) 2018-10-11
CN109153956A (zh) 2019-01-04
WO2017040995A1 (en) 2017-03-09
US20210139825A1 (en) 2021-05-13
US10927333B2 (en) 2021-02-23
EP3344746B1 (en) 2021-07-21

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