JP6989133B2 - 高スループット、単一細胞への効率的な分子送達のためのフィードバック制御されたエレクトロポレーションマイクロデバイス - Google Patents

高スループット、単一細胞への効率的な分子送達のためのフィードバック制御されたエレクトロポレーションマイクロデバイス Download PDF

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JP6989133B2
JP6989133B2 JP2018511721A JP2018511721A JP6989133B2 JP 6989133 B2 JP6989133 B2 JP 6989133B2 JP 2018511721 A JP2018511721 A JP 2018511721A JP 2018511721 A JP2018511721 A JP 2018511721A JP 6989133 B2 JP6989133 B2 JP 6989133B2
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signal
cell
impedance
detection
detection region
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JP2018529333A (ja
JP2018529333A5 (enExample
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ザーン，ジェフリー
チェン，ミンデ
シュライバー，デイヴィッド，アイ．
リン，ハオ
シャン，ジェリー，ダブリュ．
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Rutgers State University of New Jersey
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
JP2018511721A 2015-09-04 2016-09-02 高スループット、単一細胞への効率的な分子送達のためのフィードバック制御されたエレクトロポレーションマイクロデバイス Active JP6989133B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562214665P 2015-09-04 2015-09-04
US62/214,665 2015-09-04
PCT/US2016/050201 WO2017040995A1 (en) 2015-09-04 2016-09-02 High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells

Publications (3)

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JP2018529333A JP2018529333A (ja) 2018-10-11
JP2018529333A5 JP2018529333A5 (enExample) 2019-10-17
JP6989133B2 true JP6989133B2 (ja) 2022-01-05

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US (2) US10927333B2 (enExample)
EP (1) EP3344746B1 (enExample)
JP (1) JP6989133B2 (enExample)
KR (2) KR20180042422A (enExample)
CN (1) CN109153956B (enExample)
AU (1) AU2016315883B2 (enExample)
BR (1) BR112018004278B1 (enExample)
CA (1) CA2997435C (enExample)
WO (1) WO2017040995A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017176764A1 (en) 2016-04-04 2017-10-12 CyteQuest, Inc. System, device and method for electroporation of cells
WO2019125333A2 (en) * 2017-08-23 2019-06-27 Istanbul Teknik Universitesi Microfluidic system for cancer cell separation, capturing and drug screening assays
CN116987589A (zh) * 2017-10-19 2023-11-03 苏州壹达生物科技有限公司 一种流式电穿孔装置
US11485944B2 (en) 2018-04-04 2022-11-01 Hewlett-Packard Development Company, L.P. Microfluidic cellular membrane modification devices
US20190338235A1 (en) * 2018-05-01 2019-11-07 The Charles Stark Draper Laboratory, Inc. Method and Device for Exosomes Electroporation
WO2020014538A1 (en) * 2018-07-12 2020-01-16 Gpb Scientific, Llc Microfluidic methods for the preparation of cells
US12440839B2 (en) * 2018-08-21 2025-10-14 The Regents Of The University Of California High-throughput system and method for the temporary permeabilization of cells using lipid bilayers
EP3844261A2 (en) * 2018-08-31 2021-07-07 The Charles Stark Draper Laboratory, Inc. Method and apparatus for high throughput high efficiency transfection of cells
JP2022509497A (ja) * 2018-10-26 2022-01-20 カイトペン コーポレイション 電気穿孔のためのデバイス、システム、及びキット、ならびにそれらの使用方法
CN112840015A (zh) * 2018-11-12 2021-05-25 飞秒生物医学有限公司 控制细胞内递送物质的方法及装置
US12508423B2 (en) * 2019-02-04 2025-12-30 Rutgers, The State University Of New Jersey Device for tissue electrotransfer using a microelectrode
WO2020232437A1 (en) 2019-05-16 2020-11-19 CyteQuest, Inc. Devices, methods, and systems for electroporation
AU2020299541A1 (en) * 2019-07-02 2022-01-27 Kytopen Corporation Devices, systems, and kits for electro-mechanical delivery and methods of use thereof
WO2021015779A1 (en) 2019-07-25 2021-01-28 Hewlett-Packard Development Company, L.P. Cell poration and transfection apparatuses
CN110835603A (zh) * 2019-10-09 2020-02-25 遵义医科大学珠海校区 一种快速实现细胞膜可逆性损伤的装置及方法
EP3871773B1 (en) * 2020-02-27 2025-12-10 Cellix Limited A method of determining the transfection status of a plurality of cells
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation
US20220071692A1 (en) * 2020-09-08 2022-03-10 Biosense Webster (Israel) Ltd. Impedance based irreversible-electroporation (ire)
US12472495B2 (en) * 2020-10-28 2025-11-18 Northwestern University Microfluidic device for live cell manipulation and analysis
US12350682B2 (en) * 2021-03-19 2025-07-08 National Yang Ming Chiao Tung University Microelectrode device, microfluidic chip, and microfluidic examination method
EP4599040A2 (en) * 2022-10-07 2025-08-13 Rutgers, the State University of New Jersey High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells
WO2024259237A2 (en) * 2023-06-15 2024-12-19 Georgia Tech Research Corporation Targeted feedback-controlled high efficiency single cell electroporation for gene and cell therapy
WO2025128607A1 (en) * 2023-12-11 2025-06-19 Triple Ring Technologies, Inc. Method and apparatus for high-throughput transfection of cells using electroporation
WO2026015154A1 (en) * 2024-07-12 2026-01-15 Hewlett-Packard Development Company, L.P. Microfluidic device with sensor

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07184686A (ja) 1993-12-28 1995-07-25 Nec Corp 細胞活性測定方法
US7456012B2 (en) * 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
US7053063B2 (en) 1999-07-21 2006-05-30 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes in tissue
US6403348B1 (en) * 1999-07-21 2002-06-11 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
IT1320187B1 (it) * 2000-04-21 2003-11-26 Igea Srl Dispositivo e metodo di elettro-porazione che fornisce un segnalemodulato sotto il controllo continuo della elettro-permeabilizzazione
US8206903B2 (en) 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
WO2005056788A1 (en) * 2003-12-08 2005-06-23 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery into cells and tissue
US8058056B2 (en) 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
WO2006112870A1 (en) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
US7951582B2 (en) * 2005-12-19 2011-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Systems and methods for analyzing and manipulating biological samples
ITTO20060278A1 (it) 2006-04-13 2007-10-14 Silicon Biosystem S P A Metodo per la selezione e/o il processamento di particelle, in particolare cellule
US7678256B2 (en) 2006-11-03 2010-03-16 Sandia Corporation Insulator-based DEP with impedance measurements for analyte detection
AU2008213740B2 (en) * 2007-02-08 2013-02-07 Veem Ltd Marine propeller pitch adjustment means
CN102680526B (zh) * 2012-05-16 2014-07-02 清华大学 单细胞阵列微芯片及其制造、电测量和电穿孔方法

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US11987777B2 (en) 2024-05-21
AU2016315883B2 (en) 2021-01-28
KR20180042422A (ko) 2018-04-25
US20210139825A1 (en) 2021-05-13
WO2017040995A1 (en) 2017-03-09
JP2018529333A (ja) 2018-10-11
CN109153956B (zh) 2022-10-28
BR112018004278B1 (pt) 2022-05-03
CA2997435A1 (en) 2017-03-09
EP3344746A4 (en) 2019-01-16
CN109153956A (zh) 2019-01-04
BR112018004278A2 (enExample) 2018-10-02
KR20240144409A (ko) 2024-10-02
EP3344746A1 (en) 2018-07-11
EP3344746B1 (en) 2021-07-21
US20180258379A1 (en) 2018-09-13
AU2016315883A1 (en) 2018-03-29
US10927333B2 (en) 2021-02-23
CA2997435C (en) 2022-11-08

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