KR20240102017A - Composition for controlling the fruiting of ginkgo tree and method for controlling the fruiting of ginkgo tree using the same - Google Patents

Abstract

The composition for controlling ginkgo fruiting of the present invention and the method for controlling fruiting of ginkgo trees using the same reduce the fruiting of ginkgo trees by 25% to 35% without damaging the plant, causing toxicity to insect vectors, or affecting the environment, thereby preventing the loss of ginkgo fruit in autumn. Inconvenience can be improved and the cost of handling it can be significantly reduced.

Description

Composition for controlling the fruiting of ginkgo tree and method for controlling the fruiting of ginkgo tree using the same {Composition for controlling the fruiting of ginkgo tree and method for controlling the fruiting of ginkgo tree using the same}

The present invention relates to a composition for controlling ginkgo tree fruiting and a method for controlling fruiting of ginkgo trees using the same.

Street trees are shrubs planted along the road, and are mainly broad-leaved trees that are resistant to pollution, such as sycamore, willow, and ginkgo. They not only provide natural beauty to drivers and pedestrians, but also purify the air by reducing pollutants in the air. It has beneficial effects such as: In particular, ginkgo trees are very resistant to pollution such as exhaust fumes and dust from automobiles, so they do not die easily, and have an excellent air purification effect by absorbing harmful substances such as carbon dioxide and sulfur dioxide. In addition, ginkgo leaves have a good ability to absorb dust, so after dust attaches to the leaves of the ginkgo tree, it is washed away when it rains, which greatly helps in maintaining clean air in the city. Ginkgo trees also turn yellow in fall, creating a beautiful landscape. With these advantages, the ginkgo tree is quickly replacing the sycamore tree, which had large leaves and a fast growth rate and caused obstacles to surrounding buildings and was at risk of tipping over, and the willow tree, which was considered the main culprit of allergies, and was quickly replaced as a major street tree in cities including Seoul. It has been done. In the case of Seoul, as of 2020, about 35% of street trees in the city were ginkgo trees, followed by cypress trees at about 15%, zelkova trees at about 10%, and Yoshino cherry trees at about 9%.

However, in the fall, ginkgo trees fell and were trampled and crushed by passers-by, creating a foul odor in the streets, and the ginkgo trees emerged as a nuisance in the city. The average number of complaints about ginkgo fruit received from each autonomous district in Seoul is 40 to 50 per month, ranking first among single complaints in the fall season. Accordingly, the city of Seoul recently issued 'Guidelines on how to dispose of ginkgo fruit' to 25 autonomous districts and organized a task force in each district to focus on collecting ginkgo, which is consuming manpower and costs. Accordingly, various methods of processing ginkgo fruit have been studied.

Ginkgo trees have separate male and female trees, and ginkgo fruits are produced only from female trees. It is known that about 30% of planted ginkgo trees are female trees, but due to the nature of ginkgo trees, which require 15 years of growth before the results begin, it is almost impossible to distinguish between male and female trees at the seedling stage. Therefore, once the female trees are removed after planting, there is a problem of incurring enormous costs. Even in the case of early collection of ginkgo trees by mechanically shaking them, a lot of manpower and cost are required, including controlling traffic on the road and physical shock to the street trees themselves. In addition, when pesticides or high-concentration growth regulators are used, problems such as growth disturbance of the target plant, physiological crop damage, and toxicity to fertilization vectors may occur.

Recently, a method to early distinguish between male and female ginkgo trees through genetic testing using PCR has been developed, but the problem of removing already-planted street trees still remains, raising questions about their effectiveness, so fundamental measures are urgently needed. .

Korean Patent No. 10-1590548, Fertilizer composition for fruit quality improvement and ripening, registered on January 26, 2016. Korean Patent No. 10-1966792, Method for manufacturing a composition for cultivating ginkgo biloba using natural hormones and composition for cultivating ginkgo biloba produced thereby, registered on April 2, 2019. Korean Patent No. 10-2026397, Primer set for isothermal amplification for sexing of Ginkgo biloba and isothermal amplification method using the same, registered on September 23, 2019. Korean Patent No. 10-1395343, Method for identifying male and female ginkgo trees using molecular markers, registered on May 8, 2014.

The purpose of the present invention is to provide a composition for controlling ginkgo tree fruiting and a method for controlling fruiting of ginkgo trees using the same.

In order to solve the above problems, the present invention provides a composition for controlling ginkgo tree fruiting and a method for controlling fruiting of ginkgo trees using the same.

The present invention, i) fermented Houttuynia cordata;

ii) vegetable amino acids;

iii) indole-3-acetic acid (IAA); and

iv) Ascophyllum Nodosum ( Ascophyllum Nodosum ) seaweed extract; provided is a composition for controlling ginkgo fruiting, characterized in that it contains.

Houttuynia cordata used in the present invention is medicinal buckwheat ( Houttuynia) cordata ), and is named so because the leaves have a fishy smell. Houttuynia cordata, which has been traditionally used as a medicinal herb, is good for preventing arteriosclerosis and has a diuretic effect when the whole plant is made into tea and consumed. It is a dicotyledonous plant that commonly grows on Ulleungdo Island and grows well in shady and slightly damp places under roadside trees.

The vegetable amino acids of the present invention include alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline, glycine, cystine, serine, threonine, glutamine, etc., and are water-soluble and readily soluble in water. It is a growth supplement that is isolated and activates useful microorganisms in the soil and promotes the growth of crops.

Indole-3-acetic acid (IAA) of the present invention is a natural auxin, a plant hormone that is mainly produced in the cells of plant buds and young leaves and acts on the growth and cell division of plant cells. am.

Ascophyllum Nodosum of the present invention is the only species of the Ascophyllum genus of large cold-water seaweed or brown algae and grows in the North Atlantic. Ascofilm Nodosum seaweed extract contains substances such as alginic acid, amino acids, mannitol, etc., which promote cell division and growth, and contains macroelements (N, P, K), oligosaccharides, polyphenols, betaine, lignin, proteins, It is known to contain various nutrients and antioxidants such as alginic acid polysaccharide.

The fermented Houttuynia cordata may be fermented by mixing Houttuynia cordata: water: yeast: brown sugar: microorganisms in a ratio of 5-20:100:2-4:4-6:0.01-0.5. At this time, the microorganism may be one or more types of microorganisms selected from the group consisting of EM bacteria, Bacillus bacteria, photosynthetic bacteria, lactic acid bacteria, and yeast bacteria, but is not limited thereto.

The composition for controlling ginkgo fruiting includes 3 to 4 parts by weight of fermented Houttuynia cordata, 3 to 4 parts by weight of vegetable amino acids, 0.0001 to 0.01 parts by weight of indole-3-acetic acid, and Ascofilm Nodosum seaweed, based on 100 parts by weight of the composition for controlling ginkgo tree fruiting. It may contain 3 to 4 parts by weight of extract and the balance water. If less than 3 parts by weight of Houttuynia cordata fermented product is used, the fruiting efficiency is very low, and if it is used in excess of 4 parts by weight, the possibility of inhibition of ginkgo biloba physiology increases, which is undesirable. If less than 3 parts by weight of vegetable amino acids, less than 0.0001 parts by weight of indole-3-acetic acid, or less than 3 parts by weight of Ascofilm Nodosum seaweed extract are used, the efficiency of controlling ginkgo fruiting is reduced, which is not desirable, and the vegetable amino acids are 4. If more than 0.01 parts by weight of indole-3-acetic acid is used, or more than 3 parts by weight of Ascofilm Nodo Island seaweed extract is used, there is no significant change in the ginkgo fruiting control efficiency, making it uneconomical.

The composition for controlling ginkgo fruiting may further include a fermented product of Ginkgo biloba, and the fermented product can be prepared by fermenting each drug individually and mixing them, or by fermenting the mixed drugs together.

Akebia quinata is also called Akebia quinata. The fruit is a long oval-shaped berry that ripens to purple-brown in October. In oriental medicine, the roots and stems are effective in anti-inflammatory, diuretic, and analgesic effects, so they are used medicinally.

The composition for controlling ginkgo fruiting may further include gibberellic acid 4+7 (GA ₄₊₇ ). GA ₄₊₇ is a type of gibberellin, a plant hormone related to stem elongation, germination, dormancy, flowering and growth of flowers, and aging of leaves and fruits, and is a mixed compound of gibberellic acid 4 and gibberellic acid 7.

The composition for controlling ginkgo fruiting is Dictyota . dichotoma ) extract may be further included. Dictyota dichotoma ) is a marine algae that grows abundantly with several individuals tangled together in the subtidal zone of the southern coast, including the eastern coast and Jeju Island, at a depth of 1 to 5 m, and contains various active substances and nutrients.

The composition for controlling ginkgo fruiting may contain trace elements as additional ingredients. In this case, 0.0005 to 0.1 parts by weight of trace elements may be added to the composition for controlling ginkgo tree fruiting, based on 100 parts by weight. The trace elements include zinc (Zn), iron (Fe), manganese (Mn), boron (B), molybdenum (Mo), and copper (Cu). Preferably, zinc (Zn), manganese (Mn), boron (B), and molybdenum (Mo) can be used.

In addition, the composition for controlling ginkgo fruiting may further include one or more adjuvants selected from the group consisting of acidity regulators, preservatives, and surfactants.

Potassium bicarbonate can be used as the acidity regulator, and as a preservative, isothiazolone or a mixture of isothiazolone, for example, KATHON® CG/ICP preservative or Legend ^® (LEGEND ^® ) MK preservative (Rom & Haas Corporation (Rohm and HaasCorporation) or PROXEL™ BR preservatives (Avecia Corporation) can be used. Tween-20 and Tween-80 can be used as surfactants. Acidity regulators and surfactants help promote the absorption of fertilizer ingredients and protect cells, thereby promoting plant growth and individual protection, and controlling the pH to maintain an appropriate pH. In addition, preservatives allow compositions containing natural ingredients to maintain their activity without spoiling even when stored for a long period of time.

The present invention provides a method for controlling ginkgo fruiting, characterized in that the composition for controlling ginkgo tree fruiting is diluted in water at a certain ratio and subjected to irrigation treatment.

The dilution factor of the composition for controlling ginkgo fruiting can be diluted 100 to 10,000 times. The dilution factor is determined considering the environmental impact, the control effect of ginkgo tree fruiting, the amount of precipitation in the treatment area, etc., and can be diluted preferably 500 to 2000 times, and more preferably 1000 times.

The irrigation treatment of the present invention is a method of spraying a chemical solution by dripping a chemical solution into the soil. The composition for controlling ginkgo fruiting of the present invention preferably supplies the chemical solution to the soil near the roots of the ginkgo tree in the form of drip irrigation, using a drip hose. refers to a method that is connected to a container containing a diluted composition for controlling ginkgo fruiting and flows into the soil at a constant rate so that it can be continuously absorbed by the roots of the ginkgo tree.

The method for controlling ginkgo fruiting of the present invention may be to dilute the composition for controlling ginkgo tree fruiting and subject the ginkgo tree to irrigation for one month from before flowering to after flowering.

The composition for controlling ginkgo fruiting of the present invention and the method for controlling fruiting of ginkgo trees using the same reduce the fruiting of ginkgo trees by 20% to 30% without damaging the plant, causing toxicity to insect vectors, or affecting the environment, thereby preventing the loss of ginkgo fruit in the fall. Inconvenience can be improved and the cost of handling it can be significantly reduced.

Figure 1 is a photograph showing a female ginkgo tree being irrigated with a diluted composition for controlling ginkgo fruiting according to the present invention.
Figure 2 is a graph showing daily precipitation, average temperature, minimum temperature, and maximum temperature in the experimental areas Daegu (A) and Sangju (B) during the irrigation period of the composition for controlling ginkgo fruiting according to the present invention.
Figure 3 is a photograph showing a representative state of ginkgo physiological disorders that occurred during the ginkgo tree fruiting control experiment of the present invention.
Figure 4 is a diagram showing the Y-tube olfactometer and experimental principles for confirming the repellent or attractive effect of the composition for controlling ginkgo fruiting according to the present invention on insect vectors.
Figure 5 is an experiment confirming the contact toxicity of the composition for controlling ginkgo fruiting according to the present invention to insect vectors.
Figure 6 is an experiment confirming the feeding toxicity of the composition for controlling ginkgo fruiting according to the present invention to insect vectors.

In the process of developing an efficient fertilizer composition for red fruits while maintaining healthy plants, the inventors of the present invention invented a fertilizer composition for improving fruit quality and red fruits containing fermented products of mixed herbal medicines, seaweed extracts, and amino acids, and received a patent for this. (Patent No. 101590548). The fertilizer composition for red fruits was effective for ripening and red fruits of apples and pears, but when applied to ginkgo trees, there was a problem in that only about 25 to 35% of the fruit yield was induced to drop. Accordingly, in the process of researching a fruiting control composition that is effective in controlling the fruiting of ginkgo trees, an effective ginkgo tree was developed that can eliminate up to 65-75% of the fruiting volume of female ginkgo trees without causing physiological disorders of the ginkgo tree and is harmless with a 0% insect vector fatality rate. A tree fruiting control composition was developed and the present invention was completed.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms, and the content introduced here is provided to sufficiently convey the spirit of the present invention.

<Manufacture of composition for controlling ginkgo fruiting>

herbal medicine fermented product manufacturing

In a prior patent (Patent No. 101590548), the present inventor has disclosed a fertilizer composition for red fruit containing fermented products of Houttuynia cordata, Pasam, Shiho, Yangmyeong root, Eureum and oak trees, seaweed extracts, and amino acids. Among these, Houttuynia cordata is one of the key ingredients that shows effectiveness. The components contained in Houttuynia cordata were decomposed through fermentation to obtain active ingredients and were used to control the fruiting of ginkgo biloba fruits. In addition, the effect of ginkgo fruiting control on fermented herbal medicines with the addition of Houttuynia cordata and Houttuynia cordata, which is highly effective, was confirmed.

After mixing 60kg of Houttuynia cordata or 60kg of Houttuynia cordata, 50kg of brown sugar, and 30kg of yeast in 1000 L of water, 1kg of yeast was added. Instead of yeast, Bacillus, photosynthetic, lactic acid, or EM bacteria can be used. The mixture was fermented at 15°C to 30°C for more than 1 month, and then stored in a place without direct sunlight at about 5°C to 20°C for about 1 month to prepare fermented Houttuynia cordata or Houttuynia cordata.

Preparation of composition for controlling ginkgo fruiting

Fermented Houttuynia cordata, fermented Houttuynia cordata, fermented Houttuynia cordata, vegetable amino acids (Citymax Ultra AminoMax), and Ascophyllum produced above. Nodosum ) extract (Boliseco, China), Chamnetum extract (Para Jeju, Korea), indole-3-acetic acid and gibberellic acid 4+7, GA ₄₊ A composition for controlling ginkgo fruiting containing ₇ ) was prepared as shown in Table 1 below . The composition ratio in Table 1 below is expressed as a weight ratio based on 100 parts by weight of the composition for controlling ginkgo fruiting, and the remainder was made up of 100 parts by weight using water.

division fermented herbal medicine growth regulator seaweed extract vegetable amino acids Houttuynia cordata eucalyptus tree IAA GA ₄₊₇ Ascophyllum Nodosum Chamnet Batangmal Comparative Example 1 - - - - 3 - 3 Comparative Example 2 - - 0.01 - - - 3 Comparative Example 3 3 - - - - - 3 Comparative Example 4 - - 0.01 - 3 - 3 Example 1 3 - 0.01 - 3 - 3 Example 2 6 - 0.01 - 3 - 3 Example 3 3 - 0.001 - 3 - 3 Example 4 3 - 0.0001 - 6 - 3 Example 5 3 3 0.01 0.01 3 3 3

<Field application experiment of composition for controlling ginkgo fruiting>

Comparative examples and examples prepared as shown in Table 1 above were applied to female ginkgo trees to confirm the fruiting control effect. The experiment was conducted through irrigation treatment for about 2 months from before flowering to after flowering. The experiment was conducted in three locations: Buk-gu, Daegu (Kyungpook National University Daegu Campus), Dalseo-gu, Daegu (Daegu Yeongnam High School), and Sangju, Gyeongbuk (Kyungpook National University Sangju Campus). Random female trees placed within the area were selected. An experiment was conducted. For the selection of female trees, the female ginkgo trees resulting from the fall of 2019 were tested in advance and the female trees subject to the experiment were marked.

In the irrigation method, the composition was diluted 1,000 times, placed in a 20-liter sap supply plastic bag (Miseong General Materials, Korea), and installed to continuously administer it into the soil toward the roots of the ginkgo tree through a pipe as shown in Figure 1. The rate of water discharged from the tube was set to 100 ml/h and administered at a rate of about 2 L to 2.5 L/day, and a dilution of the composition for fruition control of about 20 L was administered for a total of 10 days based on the weight of the composition in the fluid supply plastic bag. set. The daily precipitation, average temperature, minimum temperature, and maximum temperature in the experimental areas Daegu (A) and Sangju (B) during the irrigation period after the start of the experiment are shown in Figure 2.

After the start of treatment, the fruiting rate and physiological disorders were evaluated for about three and a half months and are shown in Table 2. The fruiting rate was calculated by recording the number of fruits attached to the stem of the female tree at weekly intervals for one month after treatment and at monthly intervals after one month and comparing them with the fruits of the untreated control group. Physiological disorders are classified based on the overall condition of the ginkgo tree, including leaf withering, browning of leaf color, fruit color, abnormal shape, etc.: 1: no physiological disorder, 2: slightly occurs, 3: moderately occurs, 4: occurs a lot, 5 : It was evaluated as occurring very frequently.

division Dilution ratio Fruiting rate (%) Menstrual disorders (1~5) Processing start date Survey date Control (water) - 100 One April 9 July 21st Comparative Example 1 1,000 90~100 One April 9 July 21st Comparative Example 2 1,000 85~95 One April 9 July 21st Comparative Example 3 1000 70~80 One April 9 July 21st Comparative Example 4 1000 80~90 One April 9 July 21st Example 1 1000 25~35 One April 9 July 21st Example 2 1000 30~40 2 April 9 July 21st Example 3 1000 30~40 2 April 9 July 21st Example 4 1000 35~45 One April 9 July 21st Example 5 1000 25~35 One April 14 July 21st

As shown in Table 2, treatment with fermented Houttuynia cordata, plant growth regulator, or seaweed extract alone in Comparative Examples 1 to 3 did not sufficiently control ginkgo tree fruiting. Among these, treatment of fermented Houttuynia cordata alone (Comparative Example 3) showed a higher fruiting control effect than treatment with a growth regulator (Comparative Example 2), seaweed extract (Comparative Example 1) alone, or a mixture of growth regulator and seaweed extract (Comparative Example 4). It was confirmed that it represents.

In comparison, when fermented Houttuynia cordata, plant growth regulator, and seaweed extract were mixed, the fruiting rate was 20-40%, showing a remarkable fruiting control effect. Among these, Example 1 had the best fruiting control effect, and when the concentration of fermented Houttuynia cordata was increased, some physiological disorders occurred (2 points). A representative state of the physiological disorder observed at this time is shown in Figure 3.

In addition, when extracts of fermented eucalyptus tree, GA ₄₊₇ , and ginseng were added, a fruiting rate of less than 30% was observed without any physiological disorders.

<Experiment on insect vector effects of compositions for controlling ginkgo fruiting>

Y-tube olfactometer Analysis of behavioral changes in bees using

The impact of the composition for controlling ginkgo fruiting according to the present invention on the environment was confirmed. First, it was confirmed whether the composition for controlling ginkgo fruiting according to the present invention has an attracting or repelling effect on insect vectors. A Y-tube olfactometer was manufactured as shown in Figure 4, and a Teflon PTEF tube was connected to the branched glass tube and inserted into each independent glass bottle (1L). Each glass bottle was again connected to a glass bottle containing water (humidifier), and the glass bottle containing water (humidifier) was connected to a compressed air tank, and then air was flowed at a pressure of 300ml/min/arm. Afterwards, the experimental group glass bottle (Treatment jar) was treated with 20 μl of the composition of Example 1 and Whatman filter paper dried for 3 minutes, and the control jar was treated with 20 μl of water and Whatman filter paper was dried for 3 minutes. Filter paper was administered. Afterwards, worker bees (n=50) were placed at the entrance of the Y tube to confirm the attraction or repulsion effect of the composition.

As a result of the experiment, there were 21 bees in the treatment jar of the experimental group, 22 bees in the control jar, and 8 worker bees that were not selected. It was confirmed that the composition of the present invention did not show any special attraction or repulsion effect.

Confirmation of contact toxicity effects

It was confirmed whether the composition for controlling ginkgo fruiting according to the present invention exhibits contact toxicity to insect vectors. The composition of Example 1 was diluted in water 100, 300, and 1000 times as shown in Table 3, and then sprayed directly on bees in the rearing hive (about 300 bees per hive board) using a sprayer as shown in Figure 5, and incubated for 1 hour. After observation, bee behavior and dead individuals were confirmed.

Dilution ratio number of dead people behavioral disorder Control (water) - normal 100 times - normal 300 times - normal 1000 times - normal

As a result, as shown in Table 3, the composition for controlling ginkgo fruiting according to the present invention did not show any special contact disorder to vector insects. Even after observing the hive for more than a month, there was no unusual behavior of bees or an abnormal increase in the number of dead bees.

feeding Check for toxic effects

It was confirmed whether the composition for controlling ginkgo fruiting of the present invention exhibits feeding toxicity to insect vectors. The composition of Example 1 was diluted 100, 300, and 1000 times as above in sugar water (150 g of sugar, 100 ml of water) with a specific gravity of 1.27, then spread on gauze cotton and fed to each of 10 bees as shown in Figure 6. Afterwards, bee behavior and dead individuals were checked for a day. As a result, no abnormal behavior or death was observed in the bees of each experimental group, confirming that the composition of the present invention is safe for feeding against insect vectors.

Claims (10)

i) Houttuynia cordata fermented product;
ii) vegetable amino acids;
iii) indole-3-acetic acid (IAA); and
iv) Ascophyllum Nodosum ( Ascophyllum Nodosum ) A composition for controlling ginkgo fruiting, characterized in that it contains a seaweed extract. According to paragraph 1,
The composition for controlling ginkgo fruiting includes 3 to 4 parts by weight of fermented Houttuynia cordata, 3 to 4 parts by weight of vegetable amino acids, 0.0001 to 0.01 parts by weight of indole-3-acetic acid, and Ascofilm Nodosum, based on 100 parts by weight of the composition for controlling ginkgo tree fruiting. A composition for controlling ginkgo fruiting, comprising 3 to 4 parts by weight of seaweed extract and the balance water. According to paragraph 1,
The Houttuynia cordata fermented product is a composition for controlling ginkgo fruiting, characterized in that it is fermented by mixing Houttuynia cordata: water: yeast: brown sugar: microorganisms in a ratio of 5-20:100:2-4:4-6:0.01-0.5. According to paragraph 1,
A composition for controlling ginkgo fruiting, characterized in that the composition for controlling fruiting of ginkgo biloba further comprises a fermented product of ginkgo biloba. According to paragraph 1,
The composition for controlling ginkgo fruiting is characterized in that it further comprises gibberellic acid 4+7 (GA ₄₊₇ ). According to paragraph 1,
A composition for controlling ginkgo fruiting, characterized in that the composition for controlling fruiting of ginkgo biloba further comprises an extract of the ginseng root. According to paragraph 1,
The composition for controlling ginkgo fruiting further contains one or more trace elements selected from the group consisting of zinc (Zn), iron (Fe), manganese (Mn), boron (B), molybdenum (Mo), and copper (Cu). A composition for controlling ginkgo fruiting, characterized in that: According to paragraph 1,
The composition for controlling ginkgo fruiting is characterized in that it further comprises one or more adjuvants selected from the group consisting of acidity regulators, preservatives, and surfactants. A method for controlling ginkgo fruiting, characterized in that the composition for controlling ginkgo fruiting according to any one of claims 1 to 8 is diluted in water and subjected to irrigation treatment. According to clause 9,
The irrigation treatment is a ginkgo tree fruiting control method characterized in that the irrigation treatment is performed for one month from before flowering of the ginkgo tree to after flowering.