KR20240054958A - Aav 제조 방법 - Google Patents

Aav 제조 방법 Download PDF

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Publication number
KR20240054958A
KR20240054958A KR1020247000732A KR20247000732A KR20240054958A KR 20240054958 A KR20240054958 A KR 20240054958A KR 1020247000732 A KR1020247000732 A KR 1020247000732A KR 20247000732 A KR20247000732 A KR 20247000732A KR 20240054958 A KR20240054958 A KR 20240054958A
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KR
South Korea
Prior art keywords
hours
aav
glutamine
added
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247000732A
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English (en)
Korean (ko)
Inventor
플로리안 디지오파
애나 발린하스
바스티안 리위스
Original Assignee
메이라지티엑스 유케이 Ii 리미티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 메이라지티엑스 유케이 Ii 리미티드 filed Critical 메이라지티엑스 유케이 Ii 리미티드
Publication of KR20240054958A publication Critical patent/KR20240054958A/ko
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020247000732A 2021-06-17 2022-06-17 Aav 제조 방법 Pending KR20240054958A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163211877P 2021-06-17 2021-06-17
US63/211,877 2021-06-17
PCT/IB2022/000347 WO2022263930A2 (en) 2021-06-17 2022-06-17 Aav manufacturing methods

Publications (1)

Publication Number Publication Date
KR20240054958A true KR20240054958A (ko) 2024-04-26

Family

ID=82850181

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247000732A Pending KR20240054958A (ko) 2021-06-17 2022-06-17 Aav 제조 방법

Country Status (11)

Country Link
US (1) US20240279682A1 (https=)
EP (1) EP4355887A2 (https=)
JP (1) JP2024523891A (https=)
KR (1) KR20240054958A (https=)
CN (1) CN117730154A (https=)
AU (1) AU2022292164A1 (https=)
BR (1) BR112023026523A2 (https=)
CA (1) CA3222723A1 (https=)
IL (1) IL309351A (https=)
MX (1) MX2023015291A (https=)
WO (1) WO2022263930A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4713466A1 (en) * 2023-05-18 2026-03-25 Solid Biosciences Inc. Single plasmid system for aav production
WO2025063664A1 (ko) * 2023-09-18 2025-03-27 삼성바이오에피스 주식회사 재조합 aav 생산을 위한 단일 벡터 시스템

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017112948A1 (en) * 2015-12-24 2017-06-29 University Of Florida Research Foundation, Inc. Improved aav production using suspension adapted cells
MA44546B1 (fr) 2016-06-15 2021-03-31 Univ California Virus adéno-associés variants et procédés d'utilisation
KR20210093862A (ko) * 2018-09-21 2021-07-28 나이트스타엑스 리미티드 유전자 요법 벡터를 제작하기 위한 조성물 및 방법
CN109666697B (zh) * 2019-01-30 2020-03-10 广州派真生物技术有限公司 一种提高腺相关病毒产量的生产工艺
JP2023503850A (ja) * 2019-11-14 2023-02-01 バイオマリン ファーマシューティカル インコーポレイテッド 肝臓特異的遺伝子療法ベクターによる遺伝性血管浮腫の治療

Also Published As

Publication number Publication date
CN117730154A (zh) 2024-03-19
CA3222723A1 (en) 2022-12-22
JP2024523891A (ja) 2024-07-02
AU2022292164A1 (en) 2024-02-01
WO2022263930A3 (en) 2023-01-26
MX2023015291A (es) 2024-03-25
BR112023026523A2 (pt) 2024-03-05
WO2022263930A2 (en) 2022-12-22
EP4355887A2 (en) 2024-04-24
IL309351A (en) 2024-02-01
US20240279682A1 (en) 2024-08-22

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Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20240108

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20250613

Comment text: Request for Examination of Application