BR112023026523A2 - Métodos para fabricar aav - Google Patents

Métodos para fabricar aav

Info

Publication number
BR112023026523A2
BR112023026523A2 BR112023026523A BR112023026523A BR112023026523A2 BR 112023026523 A2 BR112023026523 A2 BR 112023026523A2 BR 112023026523 A BR112023026523 A BR 112023026523A BR 112023026523 A BR112023026523 A BR 112023026523A BR 112023026523 A2 BR112023026523 A2 BR 112023026523A2
Authority
BR
Brazil
Prior art keywords
cells
polynucleotide sequence
aav
methods
culture medium
Prior art date
Application number
BR112023026523A
Other languages
English (en)
Portuguese (pt)
Inventor
Ana Valinhas
Bastiaan Leewis
Florian Dziopa
Original Assignee
Meiragtx Uk Ii Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meiragtx Uk Ii Ltd filed Critical Meiragtx Uk Ii Ltd
Publication of BR112023026523A2 publication Critical patent/BR112023026523A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151Methods of production or purification of viral material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151Methods of production or purification of viral material
    • C12N2750/14152Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
BR112023026523A 2021-06-17 2022-06-17 Métodos para fabricar aav BR112023026523A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163211877P 2021-06-17 2021-06-17
PCT/IB2022/000347 WO2022263930A2 (en) 2021-06-17 2022-06-17 Aav manufacturing methods

Publications (1)

Publication Number Publication Date
BR112023026523A2 true BR112023026523A2 (pt) 2024-03-05

Family

ID=82850181

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023026523A BR112023026523A2 (pt) 2021-06-17 2022-06-17 Métodos para fabricar aav

Country Status (11)

Country Link
US (1) US20240279682A1 (https=)
EP (1) EP4355887A2 (https=)
JP (1) JP2024523891A (https=)
KR (1) KR20240054958A (https=)
CN (1) CN117730154A (https=)
AU (1) AU2022292164A1 (https=)
BR (1) BR112023026523A2 (https=)
CA (1) CA3222723A1 (https=)
IL (1) IL309351A (https=)
MX (1) MX2023015291A (https=)
WO (1) WO2022263930A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4713466A1 (en) * 2023-05-18 2026-03-25 Solid Biosciences Inc. Single plasmid system for aav production
WO2025063664A1 (ko) * 2023-09-18 2025-03-27 삼성바이오에피스 주식회사 재조합 aav 생산을 위한 단일 벡터 시스템

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017112948A1 (en) * 2015-12-24 2017-06-29 University Of Florida Research Foundation, Inc. Improved aav production using suspension adapted cells
MA44546B1 (fr) 2016-06-15 2021-03-31 Univ California Virus adéno-associés variants et procédés d'utilisation
KR20210093862A (ko) * 2018-09-21 2021-07-28 나이트스타엑스 리미티드 유전자 요법 벡터를 제작하기 위한 조성물 및 방법
CN109666697B (zh) * 2019-01-30 2020-03-10 广州派真生物技术有限公司 一种提高腺相关病毒产量的生产工艺
JP2023503850A (ja) * 2019-11-14 2023-02-01 バイオマリン ファーマシューティカル インコーポレイテッド 肝臓特異的遺伝子療法ベクターによる遺伝性血管浮腫の治療

Also Published As

Publication number Publication date
CN117730154A (zh) 2024-03-19
CA3222723A1 (en) 2022-12-22
KR20240054958A (ko) 2024-04-26
JP2024523891A (ja) 2024-07-02
AU2022292164A1 (en) 2024-02-01
WO2022263930A3 (en) 2023-01-26
MX2023015291A (es) 2024-03-25
WO2022263930A2 (en) 2022-12-22
EP4355887A2 (en) 2024-04-24
IL309351A (en) 2024-02-01
US20240279682A1 (en) 2024-08-22

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing