KR20240049218A - Soil pest insecticide composition containing natural plant extracts - Google Patents

Abstract

A preferred embodiment of the soil pest insecticidal composition containing the naturally derived plant extract of the present invention includes the steps of (a) mixing pulverized dried camphor leaves and branches with an ethanol solvent to produce an extract through pretreatment; (b) preparing a concentrated camphora extract (Camphora oil) by concentrating the extract obtained by separating the pulverized residue from the extract under reduced pressure in a reduced pressure concentration system; (c) Cinnamon oil, neem oil, lemon grass oil, eucalyptus oil, pyrethrum flower extract, and canola oil in the camphor tree concentrated extract. , Paraffin oil, complex extracts (Neem, Yangmyeong, Prickly pear, Matirne), Tea saponin, Rotenone, Jicama (Pachyrhizus erosus, Yam bean) seed extract or gourd. It consists of mixing two or more of (cake), neem-cake, starch, glucose, and diatomite; root-knot nematode ovules or It should have a control effect against larvae, etc.

Description

Soil pest insecticide composition containing natural plant extracts}

The present invention relates to a soil pest insecticidal composition containing naturally derived plant extracts, and more specifically, to a camphora concentrated extract (Camphora oil) obtained by pulverizing dried camphor leaves and branches, mixing them with an ethanol solvent, separating the extracted extract, and concentrating under reduced pressure. ), Cinnamon oil or Neem oil, Lemon grass oil, Eucalyptus oil, Pyrethrum flower extract, Canola oil, Paraffin oil ), complex extracts (Neem, Yangmyeongju, Prickly pear, Matirne), Tea tree extract (Tea saponin), Rotenone, Neem cake, Jicama (Pachyrhizus erosus, Yam bean) seed extract Or, by combining two or more ingredients such as cake, starch, glucose, and diatomite, it contains natural plant extracts that are highly effective in controlling root-knot nematode ovules or larvae, which are major pests of crops. It relates to an insecticidal composition for soil pests.

Plant parasitic nematodes have a very wide range of host plants, and infected crops such as cucumbers, watermelons, melons, tomatoes, and eggplants, which are major domestic horticultural crops, die early or suffer serious damage to crop growth and yield reduction and quality.

Among various plant parasitic nematodes, root-knot nematodes are the most difficult to control and cause the greatest damage. They cause damage to the above-ground parts of plants or to the roots of plants while living in the soil.

Root-knot nematodes include the internationally known peanut root-knot nematode (M arenaria), sweet potato root-knot nematode (Meloidogyneincognita), Java root-knot nematode (M Javanica), and carrot root-knot nematode (M hapla), which occur in horticultural crops at home and abroad. As a representative species, in Korea, it causes damage to various crops such as cucurbit crops such as melons, melons, watermelons, and cucumbers, as well as vegetables, flowers, medicinal crops, ginseng, and tree seedlings. Currently, it parasitizes and causes damage to over 2,000 types of plants. It is known.

Root-knot nematodes directly damage the roots of crops or are vectored by soil pathogens and bacteria. Root-knot nematodes (second instars) in the soil last for 35 days (May to September) to 50 days (October to April). Because they repeatedly invade the roots at intervals, infected insects, egg sacs, and hatching larvae may create holes in the root system or, in severe cases, cause tissue disintegration, aggravating the damage.

Because horticultural crops are grown year-round in Korea, recently, the occurrence and damage of root-knot nematodes is not limited to any one period but is repeated throughout the year.

In addition, root-knot nematodes, in the repeated process of invading roots, etc., secondarily attach to soil pathogens or bacteria in the body or digestive tract of infecting insects (second instars) living in the soil, or feed on them and are transmitted into plant tissue, causing damage. becomes even more serious.

Damage from root-knot nematodes is very closely related to the growth, quantity and quality of crops. The first step in which root-knot nematodes affect crops is that the root-knot nematodes repeatedly invade through the roots of the crop, so infected crops must have intact roots. The absorption of water, which is a function of the plant, is impeded, and as a result, the above-ground leaves of the plant wilt, the growth is greatly affected, and the plant dies early, or the growth yield of the plant is reduced and the quality is seriously damaged.

Control methods for these root-knot nematodes include cultural control, physical control, and chemical control. Agronomic control includes freshwater, fallow, dry soil, deep plowing, fertilized soil, rice rotation, and resistant varieties. Physical control includes heat treatment (steam, dry heat, hot water immersion) and solar disinfection, and chemical control includes Poscia. Root-knot nematodes are controlled by spraying chemical agents such as jate (nematode charcoal).

To control soil pests that damage crops, soil disinfectants are used to suppress the decrease in crop yield due to the increase in the density of parasitic nematodes, mainly organophosphorus fosthiazate, cadusafos, and batsamide (active ingredient: dazomet). Nematicides such as nematicides are widely used.

Although these chemical synthetic pesticides have recently shown a clear tendency to be converted to low-toxicity pesticides, they exhibit at least grade 4 toxicity to the human body, and are substances that remain in the soil and flow into rivers with rainwater, showing grade 3 or higher toxicity to fish. In most cases, their use has had problems threatening the dietary safety of recent consumers.

The technology behind the present invention includes Patent Publication No. 2007-0035646, “Agricultural nematode control composition containing cinnamon aldehyde isolated and purified from cinnamon” (Patent Document 1). The above background technology proposes an agricultural nematode control agent containing the filtrate obtained by pulverizing cinnamon (Cinnamomum cassia), adding 5 times its weight of 70% hydrous alcohol, extracting it at 4-40°C for 12 hours, and then filtering it. However, the above background technology has not yet disclosed a use having a nematicidal effect on root-knot nematodes with the same configuration as the present invention.

Patent Publication No. 2007-0035646 “Agricultural nematode control composition containing cinnamon aldehyde isolated and purified from cinnamon”

The present invention is intended to solve the above problems, by controlling soil pests using plant extracts of natural origin, so that it is harmless to plants or the human body and has a control effect against soil pests, especially root-knot nematode ovaries or larvae. The purpose is to provide a soil pest insecticide composition containing naturally derived plant extracts.

The present invention includes the steps of (a) mixing pulverized dried camphor leaves and branches with an ethanol solvent to produce an extract through pretreatment; (b) preparing a concentrated camphora extract (Camphora oil) by concentrating the extract obtained by separating the pulverized residue from the extract under reduced pressure in a reduced pressure concentration system; (c) The concentrated camphor extract contains cinnamon oil, neem oil, lemon grass oil, eucalyptus oil, pyrethrum flower extract, and canola oil. ), paraffin oil, complex extracts (Neem, Yangmyeong, Prickly pear, Matirne), Tea saponin, Rotenone, Jicama (Pachyrhizus erosus, Yam bean) seed extract or Contains naturally derived plant extracts, comprising the step of mixing two or more of cake, neem-cake, starch, glucose, and diatomite. The purpose is to provide a method for manufacturing a soil pest insecticidal composition.

In addition, the ground product is mixed with an ethanol solvent, the temperature is set to 55 to 65°C in a shaking incubator, and the extract is generated by setting the temperature to 130 to 160 rpm and incubating for 23 to 25 hours. The aim is to provide a method for manufacturing a soil pest insecticidal composition containing naturally derived plant extracts, which is characterized in that:

In addition, in step (b), the heating bath temperature of the rotary evaporator is 40 to 60 ℃, and the circulation temperature is 8 to 11 ℃. The aim is to provide a soil pest insecticidal composition containing plant extracts.

Also, in step (b). Camphora oil is extracted and separated by mixing 10-20% by weight of extract with 80-90% by weight of solvent, and 85% of the primary extract is recovered based on 100% of the separated weight. The aim is to provide a soil pest insecticide composition containing naturally derived plant extracts, which is characterized by recovering the concentrated extract through a concentration system.

Additionally, in step (c), lemon grass oil, eucalyptus oil, Pyrethrum flower extract, canola oil, paraffin oil, and complex extracts (Neem, When mixing two of the seed extracts of Matirne, Tea saponin, Rotenone, and Jicama (Pachyrhizus erosus, Yam bean) with concentrated camphor extract, a surfactant is mixed and stirred first. The aim is to provide a soil pest insecticidal composition containing naturally derived plant extracts, which is made into a liquid by adding purified water or alcohol as an extender and stirring it a second time.

In addition, in step (c), camphor tree concentrated extract, jicama (Pachyrhizus erosus, Yam bean) seed extract, neem-cake, starch, glucose, and diatomite are added to the camphor tree concentrate. The object of the present invention is to provide a soil pest insecticide composition containing a naturally derived plant extract, which is mixed with an extract to form granules of a certain size.

In addition, in step (c), the jicama (Pachyrhizus erosus, Yam bean) seed extract is prepared by crushing the jicama seeds, adding the weight of hexane and the weight of the jicama seeds in a ratio of 20 times the weight ratio, and then leaving it at room temperature for 24 hours. The object is to provide a soil pest insecticidal composition containing naturally derived plant extracts, which are formed by extraction during the process.

In addition, the object is to provide a soil pest insecticidal composition containing a naturally derived plant extract, which is manufactured by any of the above methods.

The soil pest insecticidal composition containing naturally derived plant extracts of the present invention controls soil pests using naturally derived plant extracts, and is harmless to plant crops or the human body, but has a control effect against soil pests, especially root-knot nematode ovules or larvae. It has a very useful effect.

Below, the present invention will be described in detail with reference to the attached presented examples, but the presented examples are illustrative for a clear understanding of the present invention, and the present invention is not limited thereto.

Hereinafter, the technical configuration of the present invention will be described in detail according to preferred embodiments.

First, dried camphor leaves and branches are pulverized and mixed in an ethanol solvent to produce an extract through pretreatment (a). The extract is separated from the extract and concentrated under reduced pressure in a reduced pressure concentration system to produce a concentrated camphor extract (Camphora oil). Be prepared (b).

Camphor tree is an evergreen broad-leaved tree of the dicotyledonous plant Camphor order Camphoridae and is distributed in Korea (Jeju), Japan, Taiwan, China, Indonesia, and Sumatra. It is also called camphor tree or longevity tree, and grows well in deep, rich soil or shady places. It is about 20m high and 2m in diameter. The oil obtained by steam distillation of lumber, branches, leaves, and roots is called camphor, which is used as a raw material for making fragrances, insect repellent, and tonics.

Camphor leaves and branches are dried and ground to prepare, and the dried camphor leaves and branches are mixed with a solvent to produce an extract. The extraction conditions are that the ground product is mixed with an ethanol solvent and the temperature is adjusted in a shaking incubator. It is preferable to set the temperature to 55 to 65°C, set it to 130 to 160 rpm, and incubate for 23 to 25 hours to produce the extract. The conditions for the extraction experiment are as follows.

① Extraction conditions: 60℃, 24hr

② Extraction solvent: 94% ethanol (EtOH)

③ Extraction method

- Grind 200g of dry samples of dried camphor leaves and branches, place them in a 2L capped bottle, and fill with solvent to make the final volume 2L.

- Shake the ground dry sample and the solvent to mix well, and incubate in a shaking incubator at 60°C and 150rpm for 24 hours to produce the extract.

The extract in step (a) is separated from the extract and pulverized residue with gauze, and the separated extract is filtered with filter paper to prepare a concentrated camphora extract (Camphora oil).

At this time, the concentrated extract is concentrated using a rotary evaporator. The heating bath temperature of the rotary evaporator is 40 to 60°C, and the circulation temperature is 8. Concentrate at ~11°C.

In the reduced-pressure concentration system, the concentration process is stopped when the volume (vol.) of the reaction sample (sample) reaches about 1/10, and extraction is performed at the ratio below.

That is, extract and separate by mixing 10-20% by weight of extract with 80-90% by weight of solvent.

⇒ Recover about 85% of the primary extract

⇒ The first recovered extract is recovered as concentrated extract through a concentration system.

⇒ A yield of about 8.8% was confirmed.

Afterwards, the concentrated extract of camphor tree was mixed with cinnamon oil, neem oil, lemon grass oil, eucalyptus oil, pyrethrum flower extract, canola oil, Paraffin oil, complex extracts (Neem, Yangmyeong, Prickly Pear, Matirne), Tea saponin, Rotenone, Jicama (Pachyrhizus erosus, Yam bean) seed extract or gourd ( Mix two or more of Cake, Neem-cake, Starch, Glucose, and Diatomite to produce a soil pest insecticidal composition containing naturally derived plant extracts (c) .

In the camphor tree concentrated extract, cinnamon oil or neem oil, lemon grass oil, eucalyptus oil, pyrethrum flower extract, canola oil, and paraffin. Oil (Paraffin oil), complex extracts (Neem, Yangmyeongju, Prickly pear, Matirne), Tea saponin, Rotenone, Jicama (Pachyrhizus erosus, Yam bean) seed extract or cake ), Neem-cake, Starch, Glucose, and Diatomite can be mixed in various dosage forms such as liquid or granule.

In particular, cinnamon oil or neem oil, lemon grass oil, eucalyptus oil, Pyrethrum flower extract, canola oil, and paraffin oil. ), camphor tree concentrated extract containing at least two of the following: complex extracts (Neem, Yangmyeong, Prickly pear, Matirne), Tea tree extract (Tea saponin), Rotenone, and Jicama (Pachyrhizus erosus, Yam bean) seed extract. When mixing, the surfactant is mixed and stirred first, and purified water or alcohol is added as an extender and stirred secondly to prepare a liquid.

First, add vegetable oil (jicama extract, camphor oil, pyrethrum extract, cinnamon oil, neem oil, etc.) and surfactant according to the input ratio and stir sufficiently for about 24 hours. Add extender to the sufficiently stirred vegetable oil and surfactant composition. Add phosphorus purified water or alcohol and complete secondary stirring for at least 2 hours.

Additionally, complex extracts, etc. can be added and stirred for about 2 hours to complete the process.

In addition, concentrated camphor extract, tea saponin, jicama (Pachyrhizus erosus, Yam bean) seed extract or cake, neem cake, starch, glucose, and diatomaceous earth (Diatomite) can also be mixed with camphor tree concentrated extract to form granules of a certain size.

Prepare a mixed composition by mixing the above ingredients, adjust the size to a diameter of 3 to 5 mm or less using a production equipment ventilation machine, and prepare a dosage form according to the composition. Using a production equipment purifier (circular punch), mechanical pressure is 5 to 10 mm. A formulation according to the composition can be prepared under MPa (50-100 kgf/cm2) conditions.

* T80 (Polyoxyethylene sorbitan monooleate)

As shown in Table 1 above, it can be prepared in liquid or granular form by mixing the composition.

In particular, to increase the yield, jicama (Pachyrhizus erosus, Yam bean) seed extract or cake is formed by crushing jicama seeds, adding 20 times the amount of hexane as jicama seeds, and then extracting at room temperature for 24 hours. You can.

The following experiment was conducted to determine the extraction conditions and yield of jicama seeds.

Jicama Seed Supercritical Extraction

Jicama seeds were crushed and extracted at 50°C under different pressures (200-400 bar). The weight of each extract and the extracted meal was measured to confirm the yield. The results are shown in Table 2.

As shown in Table 2 above, the yield of jicama (Pachyrhizus erosus, Yam bean) seed extract or cake was 20.75% at 400 bar.

Jicama Seed Hexane Extraction

To increase the yield, the jicama seeds were crushed, 20 times the amount of hexane as the jicama seed was added, extracted at room temperature for 24 hours, and the weight of the extract and the extracted meal were measured to confirm the yield.

As shown in Table 3, the yield was 24%.

<Testing the insecticidal effect of insecticidal compositions against soil pest root-knot nematodes>

Method of separating root-knot nematode eggs and larvae

As shown in Figure 1, the zucchini roots infected with root knot nematodes and forming nodules were washed several times with water, cut into pieces about 1 cm in size, placed in a 1 L glass bottle, and then added with 0.5% sodium hypochlorite. Pour in enough to submerge the roots, close the lid, and shake with a shaker at 150-180 rpm for 1 minute. Then, place a double layer of 200 mesh sieve in a 500 mesh sieve, pour the vigorously shaken root solution into it, and quickly dissolve with sodium hypochlorite with tap water. After rinsing, it was collected in a beaker.

This separation process is repeated two or more times, and nematode eggs remain in the lower 500 mesh sieve, and only roots and other debris remain in the upper 200 mesh sieve. The nematode eggs isolated in this way were separated and used.

To hatch larvae from nematode eggs, spread two sheets of toilet paper over a sieve with a diameter of 10cm and a 50mesh scale.

After placing the body in a thin funnel filled with water, 2 to 3 days later, the eggs hatch and come down through the rubber tube of the funnel. These hatched larvae were collected and used in experiments.

Test of insecticidal effect on root-knot nematode larvae using insecticidal composition

As shown in Table 1, a soil pest insecticidal composition containing natural plant extracts in granular or liquid form was used to test the insecticidal effect on root-knot nematode larvae.

1. Liquid

For isolated second-stage root-knot nematode larvae, the mixed composition was diluted to 500-fold and 250-fold, respectively, including the standard amount (1,000-fold), and then observed on the 1st and 3rd days to investigate the insecticidal rate on the 3rd day.

As a result of the insecticidal effect test, 2% pyrethrine extract, 20% rotenone, 5% cinnamon oil, 5% lemon grass, 2% jicama seed extract, T80 5 It was confirmed that the insecticidal rate against root-knot nematode larvae was more than 65% at 250 and 500 times in the composition containing %.

2. Admission

For the isolated second stage root-knot nematode larvae, a soil mixed treatment agent was used, samples were treated at 9 mg/1 plate based on 2 kg/150 pyeong, and observed on the 1st, 3rd, and 5th days, and the insecticide rate was investigated on the 5th day.

As a result of an insecticidal effect test, a composition containing 40% tea saponin, 20% neem cake, 20% jicama meal, 10% diatomite, and 10% glucose was found to kill root-knot nematode larvae. It was confirmed that the insecticide rate was over 60%.

The insecticide rate was calculated using Equation 1.

As shown in Table 4, the insecticidal rate against root-knot nematode larvae, a major soil pest, was examined by combining the optimal composition according to the formulation. In liquid form, pyrethrine extract, cinnamon oil, and Remo were used. It showed significant insecticidal effects on lemon grass oil, rotenone, and jicama seed extract, and on the other hand, tea saponin, neem cake, and jicama bean were used. cake, diatomite, and glucose showed significant insecticidal effects.

The soil pest insecticidal composition containing naturally derived plant extracts of the present invention as described above controls soil pests using naturally derived plant extracts, and is harmless to plants or the human body, but is also harmful to soil pests, especially root-knot nematode ovules or larvae. It has a very useful effect in having a pest control effect.

Claims (1)

(a) mixing pulverized dried camphor leaves and branches with ethanol solvent to produce an extract through pretreatment;
(b) preparing a concentrated camphora extract (Camphora oil) by concentrating the extract obtained by separating the pulverized residue from the extract under reduced pressure in a reduced pressure concentration system;
(c) In the camphor tree concentrated extract,
In the soil pest insecticidal composition containing naturally derived plant extracts comprising the step of mixing two seed extracts: tea saponin and jicama (Pachyrhizus erosus, Yam bean),
In step (a), the pulverized product is mixed with an ethanol solvent, the temperature is set to 55-65°C in a shaking incubator, and the mixture is incubated for 23-25 hours by setting at 130-160 rpm. to produce an extract,
In step (b), the heating bath temperature of the rotary evaporator is 40 to 60 ℃, and the circulation temperature is 8 to 11 ℃,
In step (c), when mixing the two seed extracts, tea tree extract and jicama (Pachyrhizus erosus, Yam bean), with camphor tree concentrated extract, a surfactant is mixed and stirred first, and purified water is added as an extender and stirred second time. It is made in liquid form,
In step (c), jicama (Pachyrhizus erosus, Yam bean) seed extract is mixed with the camphor tree concentrated extract to form granules of a certain size,
In step (c), the jicama (Pachyrhizus erosus, Yam bean) seed extract is formed by grinding the jicama seeds, adding hexane at a ratio of 20 times the weight of the jicama seeds, and then extracting at room temperature for 24 hours. A soil pest insecticidal composition containing naturally derived plant extracts, characterized in that: