KR20240037594A - Composition for removing elderly smell comprising extract of Inula japonica - Google Patents

Abstract

The present invention relates to a composition for removing elderly odor containing Geumbulcho extract, which has the effect of removing elderly odor by removing nonenal, and has antioxidant and wrinkle-improving effects, making it a natural material that can be used in cosmetics, food, pharmaceuticals, etc. It can be applied to various fields.

Description

Composition for removing elderly smell comprising extract of Inula japonica}

The present invention relates to a composition for removing elderly odor containing Geumbulcho extract as an active ingredient.

Recently, as the elderly population has increased, the need and diversity of beauty and personal health care products for the silver generation are being demanded.

In old age, cis-9-hexadecenoic acid, a fatty acid contained in sebum, is oxidized or decomposed by skin bacteria to produce 2-Nonenal, which causes a characteristic senile appearance. An odor occurs. Nonenal is an unsaturated aldehyde substance, including 3-hexyl-2-propenal, 3-hexylacrolein, heptylideneacetaldehyde, and beta-hexyl. It is also called β-hexylacrolein, α-nonenyl aldehyde, nonylenic aldehyde, or trans-2-nonenal.

Nonenal is mainly produced in elderly people, but it also occurs in patients suffering from chronic diseases or having difficulty moving. As metabolism is not smooth, more waste accumulates on the skin, and as unsaturated fatty acids are broken down in these sebaceous glands, a lot of nonenal is produced, causing a musty smell. Therefore, there is a need to develop natural materials to deodorize elderly people by removing nonenal, the main cause of elderly people's odor.

Meanwhile, Inula japonica is a plant in the Asteraceae family, also called Daesim or Jeokjeokgeum, and is a wild plant distributed in East Asia, including Korea, Japan, and China. Geumbulcho circulates and releases congested energy and is used for phlegm, coughing, hiccups, belching, asthma, chronic bronchitis, acute pleurisy, and stuffy symptoms such as feeling like the chest is always full. It is also used for morning sickness, breast cancer, and breast cancer. The components of Geumbulcho flowers include Nepetin, patuletin 7-O-(6''-isobutyryl)glucoside, and patuletin 7. -O-[6''-(2-methylbutyryl)]glucoside (patuletin 7-O-[6''-(2-methylbutyryl)]glucoside), patuletin 7-O-(6''-iso Contains flavonoids such as patuletin 7-O-(6''-isovaleryl)-glucoside, patulitrin, patuletin, and luteolin, and contains poly It is known to play a major role in radical scavenging ability as it contains a large amount of phenol and flavonoid components.

Meanwhile, there has been no research on the effectiveness of Geumbulcho extract in removing odor in the elderly.

Korean Patent No. 10-1554537 Korean Patent No. 10-2205201

The purpose of the present invention is to provide a composition for removing odor in the elderly containing Inula japonica extract as an active ingredient.

The present invention provides a composition for removing odor in the elderly containing Inula japonica extract as an active ingredient.

The Geumbulcho extract is a flower extract of Geumbulcho.

The Geumbulcho extract may be extracted with water, C ₁ -C ₄ alcohol, or a mixed solvent thereof, preferably with ethanol, more preferably with 30% to 70% ethanol.

The causative agent of the senile odor may be any one or more selected from the group consisting of nonenal, octanal, nonanal, and decanal, and is preferably nonenal (trans-2-nonenal).

In addition, in another aspect, the present invention includes the steps of immersing Geumbulcho flowers in water, C ₁ to C ₄ alcohol, or a mixed solvent thereof; Filtering Geumbulcho flower extract to remove impurities; and concentrating the Geumbulcho flower extract from which impurities have been removed. It provides a method for producing a composition for removing odor of the elderly comprising Geumbulcho flower extract as an active ingredient.

In addition, in another aspect, the present invention provides a cosmetic composition for removing elderly odor containing Geumbulcho flower extract prepared by the above manufacturing method as an active ingredient.

The composition for removing geriatric odor according to the present invention has the effect of removing geriatric odor by removing nonenal, and in addition, it has antioxidant and wrinkle-improving effects, so it can be used in a variety of ways, such as cosmetics, health functional foods, and pharmaceuticals.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for removing odor in the elderly containing Inula japonica extract as an active ingredient.

It is preferable that the Geumbulcho extract is a Geumbulcho flower extract.

The Geumbulcho extract may be extracted with water, C ₁ to C ₄ alcohol, or a mixed solvent thereof, preferably with ethanol, and more preferably with 30% to 70% ethanol.

The causative agent of the senile odor may be any one or more selected from the group consisting of nonenal, octanal, nonanal, and decanal, and is preferably nonenal (trans-2-nonenal).

In addition, in another aspect, the present invention includes the steps of immersing Geumbulcho flowers in water, C ₁ to C ₄ alcohol, or a mixed solvent thereof; Filtering Geumbulcho flower extract to remove impurities; and concentrating the Geumbulcho flower extract from which impurities have been removed. It provides a method for producing a composition for removing odor of the elderly comprising Geumbulcho flower extract as an active ingredient.

In addition, in another aspect, the present invention provides a cosmetic composition for removing elderly odor containing Geumbulcho flower extract prepared by the above manufacturing method as an active ingredient.

The cosmetic composition of the present invention can be prepared in the form of a general emulsified formulation and solubilized formulation. Cosmetics in emulsified formulations include nourishing lotions, creams, and essences, and cosmetics in solubilized formulations include flexible lotions. In addition, in addition to cosmetics, it can be manufactured in the form of an adjuvant that can be applied topically or systemically in the field of dermatology by containing a dermatologically acceptable medium or base.

In addition, the formulation of the cosmetic composition in the present invention is not limited, but includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, and essence. , pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, spray, and powder.

In the cosmetic composition, in each formulation, other ingredients other than the active ingredient may be appropriately mixed depending on the type of formulation or purpose of use, etc., within the range that does not impair the purpose according to the present invention.

In addition, depending on the quality or function of the final product, fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, and interfaces are commonly used in the industry. Activators, water, ionic or non-ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, and commonly used in cosmetic compositions. It may additionally include auxiliaries commonly used in the field of cosmetics or dermatology, such as any other ingredients.

However, it is desirable to select the auxiliaries and their mixing ratio appropriately so as not to affect the desirable properties of the cosmetic composition according to the present invention.

The composition of the present invention can be applied to the production of not only cosmetics, but also food, medicine, and household goods.

Hereinafter, the present invention will be described in detail through the following examples and experimental examples. However, this is only presented to aid understanding of the present invention and the present invention is not limited thereto.

[Example 1] Preparation of 30% ethanol Geumbulcho flower extract

100 g of Geumbulcho flowers were immersed in 1 liter of 30% ethanol for 24 hours and then concentrated using an evaporator to prepare 30% ethanol Geumbulcho flower extract.

[Example 2] Preparation of 50% ethanol Geumbulcho flower extract

100 g of Geumbulcho flowers were immersed in 1 liter of 50% ethanol for 24 hours and then concentrated using an evaporator to prepare 50% ethanol Geumbulcho flower extract.

[Example 3] Preparation of 70% ethanol Geumbulcho flower extract

100 g of Geumbulcho flowers were immersed in 1 liter of 70% ethanol for 24 hours and then concentrated using an evaporator to prepare 70% ethanol Geumbulcho flower extract.

[Comparative Example] Preparation of 70% ethanol Changi extract

100 g of Changja was immersed in 1 liter of 70% ethanol for 24 hours and then concentrated using an evaporator to prepare a 70% ethanol Geumbulcho flower extract.

[Experimental Example 1] GC-MS component analysis

Component analysis of the Geumbulcho flower extracts prepared in Examples 1 to 3 was performed using a gas chromatograph-mass spectrometer (GC-MS).

Octane(%) Sulfurous acid(%) Eugenol(%) Benzofuranone(%) Example 1 59.7 40.5 - 18.9 Example 2 71.6 48.2 67.7 - Example 3 82.9 84.6 95.0 -

As shown in Table 1, the active ingredients Octane, Sulfurous acid, Eugenol, and Benzofuranone that exhibit antioxidant effects were confirmed in the flower extracts of Examples 1 to 3. It has been done. In Example 1, octane, sulfurous acid, and benzofuranone components were detected, and in Examples 2 and 3, octane, sulfurous acid, and eugenol components were detected. In Example 3, it was confirmed that it contained the most active ingredients as 82.9% of octane, 84.6% of sulfurous acid, and 95.0% of eugenol were detected.

[Experimental Example 2] Analysis of DPPH radical scavenging ability

The Geumbulcho flower extract prepared in Examples 1 to 3 was prepared at a concentration of 50 ㎕/㎖, and then 100 ㎕ of the diluted Geumbulcho flower extract was mixed with 900 ㎕ of 0.12 mM DPPH solution and left in the dark for 30 minutes. Absorbance was measured at 517 nm using a UV-vis spectrophotometer. Ascorbic acid was used as a control, and ascorbic acid was diluted to a concentration of 0.1 ㎕/㎖.

IC ₅₀ Ascorbic acid 2.07 Example 1 3.30 Example 2 2.90 Example 3 2.98

As shown in Table 2, as a result of the analysis of antioxidant efficacy according to DPPH radical scavenging ability, the 50% ethanol extract (Example 2) and the 70% ethanol extract (Example 3) had IC ₅₀ similar to that of ascorbic acid as a control. It was confirmed that the higher the value, the higher the antioxidant efficacy.

[Experimental Example 3] Analysis of efficacy in removing elderly odor

1 g of the Geumbulcho flower extract of Examples 1 to 3 and the Changi extract of Comparative Example 1 were each diluted in 100 ml of ethanol, then mixed with 100 ㎕ of Nonenal standard solution and dispersed for 30 minutes. 150 μl each of Fehling solutions A and B were added and reacted at 50°C for 30 minutes, and then the absorbance was measured at 300 nm using a UV-vis spectrophotometer.

Absorbance (300 nm) 2-Nonenal removal rate (%) control group 1.117 - Example 1 0.383 65.8 Example 2 0.369 66.9 Example 3 0.402 64.2 Comparative example 0.175 31.7

As shown in Table 3, as a result of the nonenal (trans-2-nonenal) Felling reaction, the nonenal removal rate of the comparative example Changja extract was 31.7%, while the Geumbulcho flower extract of Examples 1 to 3 of the present invention had a removal rate of about 65%. % of nonenal removal ability, it can be seen that the nonenal removal ability of Geumbulcho flower extract is excellent, and the efficacy of Geumbulcho flower extract in removing elderly odor according to ethanol extraction concentration was confirmed to be similar.

[Experimental Example 4] Collagenase inhibitory activity analysis

Collagenase is an enzyme that hydrolyzes collagen. Collagen is one of the main structural proteins that make up skin tissue, and plays a role in protecting cells, including maintaining their structure and providing strength. When collagen, which makes up most of the dermis layer within the skin, is broken down, skin elasticity decreases and skin aging, such as the formation of wrinkles and pores, is accelerated. The progression of skin aging can be suppressed by inhibiting the collagen decomposition reaction of collagenase. Collagenase cleaves 4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg into colored 4-Phenylazobenzyloxycarbonyl-Pro-Leu-OH and colorless Gly-Pro-D-Arg-OH. Since the orange-yellow colored acyl peptide is highly soluble in ethyl acetate, 4-Phenylazobenzyloxycarbonyl-Pro-Leu-OH can be extracted from the acidified mixture using ethyl acetate and quantified using a spectrophotometer. You can.

To evaluate the wrinkle-improving ability of the Geumbulcho flower extracts of Examples 1 to 3, collagenase inhibitory activity was analyzed, and ascorbic acid was used as a positive control.

First, 4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg, which is used as a substrate for measuring collagenase activity, was dissolved in collagenase buffer (pH 7.5) to create a 1.2 mg/ml substrate solution. Manufactured. An enzyme solution (0.4 mg/ml) was prepared by adding collagenase to TES buffer (50mM, pH 7.4) containing 0.35mM calcium chloride (CaCl ₂ ). After mixing 0.25 ml of substrate solution and 0.1 ml of sample solution, add 0.15 ml of collagenase (0.4 mg/ml) and react at room temperature for 20 minutes. When the reaction was completed, 0.25 ml of 20% citric acid was added to stop the reaction, and then 1.2 ml of ethyl acetate was added and stirred at 120 rpm for 10 minutes. After centrifugation, the supernatant was taken and the absorbance was measured at a wavelength of 320 nm. Collagenase inhibitory activity was expressed as the rate of decrease in absorbance of the sample solution added and not added.

Inhibition rate (%) = (1-absorbance of sample added/absorbance of no sample added) x 100

Collagenase activity inhibition rate (%) Ascorbic acid 99.7 Example 1 85.3 Example 2 75.1 Example 3 90.9

Referring to Table 4, as a result of analyzing the collagenase activity inhibition effect of Geumbulcho flower extract, the collagenase activity inhibition rate of ascorbic acid, a positive control, was found to be 99.7%, and the collagenase activity inhibition rate of Examples 1 to 3 was found to be 99.7%. The activity inhibition rate was confirmed to be slightly lower than that of ascorbic acid, but overall high. Among them, Example 3 showed a collagenase activity inhibition rate of over 90%, showing that the wrinkle improvement effect was excellent.

In summary, the composition containing Geumbulcho flower extract according to the present invention has excellent antioxidant, wrinkle improvement, and nonenal removal effects and is expected to be applicable to a variety of natural functional materials.

As described above, the present invention has been described with reference to the embodiments shown in the drawings, but this is only for illustrating the invention, and those skilled in the art may make various modifications or equivalents from the detailed description of the invention. It will be appreciated that one embodiment is possible. Therefore, the true scope of rights of the present invention must be determined by the technical spirit of the patent claims.

Claims (9)

Composition for removing odor from the elderly containing Inula japonica extract as an active ingredient
The composition for removing elderly odor according to claim 1, wherein the extract of Geumbulcho is a flower extract of Geumbulcho.
The composition for removing elderly odor according to claim 1, wherein the flower extract of Geumbulcho is extracted with water, C ₁ to C ₄ alcohol, or a mixed solvent thereof.
The composition for removing elderly odor according to claim 1, wherein the flower extract of Geumbulcho is extracted with ethanol.
The composition for removing elderly odor according to claim 4, wherein the ethanol is 30% to 70% ethanol.
The composition for removing odor of the elderly according to claim 1, wherein the substance causing the odor of the elderly is at least one selected from the group consisting of nonenal, octanal, nonanal, and decanal.
The composition for removing odor of the elderly according to claim 6, wherein the causative agent of the odor of the elderly is nonenal (trans-2-nonenal).
Immersing Geumbulcho flowers in water, C ₁ to C ₄ alcohol, or a mixed solvent thereof;
Filtering Geumbulcho flower extract to remove impurities; and
Method for producing a composition for removing elderly odor containing Geumbulcho flower extract as an active ingredient, comprising: concentrating the flower extract of Geumbulcho from which impurities have been removed;
A cosmetic composition for removing elderly odor containing Geumbulcho flower extract prepared by the manufacturing method according to paragraph 8 as an active ingredient.