KR20240019808A - 생물학적 샘플을 분석하기 위한 시스템 및 방법 - Google Patents

생물학적 샘플을 분석하기 위한 시스템 및 방법 Download PDF

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Publication number
KR20240019808A
KR20240019808A KR1020247000613A KR20247000613A KR20240019808A KR 20240019808 A KR20240019808 A KR 20240019808A KR 1020247000613 A KR1020247000613 A KR 1020247000613A KR 20247000613 A KR20247000613 A KR 20247000613A KR 20240019808 A KR20240019808 A KR 20240019808A
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South Korea
Prior art keywords
nucleic acid
molecules
subset
cdna
derivatives
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KR1020247000613A
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Korean (ko)
Inventor
피어 페데리코 게라르디니
타룬 쿠마르 쿠라나
알리 아가
일-쉬안 우
필리즈 고르페 야사르
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셀라노메, 인크.
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Publication of KR20240019808A publication Critical patent/KR20240019808A/ko
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020247000613A 2021-06-11 2022-06-10 생물학적 샘플을 분석하기 위한 시스템 및 방법 Pending KR20240019808A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163209544P 2021-06-11 2021-06-11
US63/209,544 2021-06-11
PCT/US2022/033116 WO2022261507A1 (en) 2021-06-11 2022-06-10 Systems and methods for analyzing biological samples

Publications (1)

Publication Number Publication Date
KR20240019808A true KR20240019808A (ko) 2024-02-14

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Family Applications (1)

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KR1020247000613A Pending KR20240019808A (ko) 2021-06-11 2022-06-10 생물학적 샘플을 분석하기 위한 시스템 및 방법

Country Status (11)

Country Link
US (1) US20240301465A1 (https=)
EP (1) EP4352286A4 (https=)
JP (1) JP2024520804A (https=)
KR (1) KR20240019808A (https=)
CN (1) CN117813424A (https=)
AU (1) AU2022287983A1 (https=)
BR (1) BR112023025731A2 (https=)
CA (1) CA3220725A1 (https=)
IL (1) IL308886A (https=)
MX (1) MX2023014290A (https=)
WO (1) WO2022261507A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム, インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
CN120112795A (zh) * 2022-07-19 2025-06-06 赛拉诺姆公司 使用可降解水凝胶的单细胞蛋白质组学
WO2024253995A1 (en) * 2023-06-09 2024-12-12 Illumina, Inc. Methods of sequencing dna using flow cells containing multiple surfaces
WO2025128916A1 (en) 2023-12-13 2025-06-19 Cellanome, Inc. Devices and methods for analyzing biological samples
WO2025229401A2 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for screening of biological components
WO2025231451A1 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for analysis of cells

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5331476B2 (ja) * 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
WO2014060483A1 (en) * 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015065924A2 (en) * 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
SG11201609053YA (en) * 2014-04-29 2016-11-29 Illumina Inc Multiplexed single cell gene expression analysis using template switch and tagmentation
US10570447B2 (en) * 2014-05-16 2020-02-25 Illumina, Inc. Nucleic acid synthesis techniques
EP3350732B1 (en) * 2015-09-15 2024-07-24 Takara Bio USA, Inc. Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same
FI3662083T3 (fi) * 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
WO2019203728A1 (en) * 2018-04-20 2019-10-24 National University Of Singapore Linearization and labeling of single-stranded dna for optical sequence analysis
EP3844304B1 (en) * 2018-08-28 2024-10-02 10X Genomics, Inc. Methods for generating spatially barcoded arrays
WO2020190509A1 (en) * 2019-03-15 2020-09-24 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
EP4263859A1 (en) * 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis

Also Published As

Publication number Publication date
AU2022287983A1 (en) 2023-12-14
CN117813424A (zh) 2024-04-02
US20240301465A1 (en) 2024-09-12
EP4352286A1 (en) 2024-04-17
WO2022261507A1 (en) 2022-12-15
JP2024520804A (ja) 2024-05-24
BR112023025731A2 (pt) 2024-02-27
EP4352286A4 (en) 2025-04-23
CA3220725A1 (en) 2022-12-15
IL308886A (en) 2024-01-01
MX2023014290A (es) 2024-02-27

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Patent event date: 20240108

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Patent event code: PA02012R01D

Patent event date: 20250610

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