CA3220725A1 - Systems and methods for analyzing biological samples - Google Patents

Systems and methods for analyzing biological samples Download PDF

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Publication number
CA3220725A1
CA3220725A1 CA3220725A CA3220725A CA3220725A1 CA 3220725 A1 CA3220725 A1 CA 3220725A1 CA 3220725 A CA3220725 A CA 3220725A CA 3220725 A CA3220725 A CA 3220725A CA 3220725 A1 CA3220725 A1 CA 3220725A1
Authority
CA
Canada
Prior art keywords
nucleic acid
molecules
subset
cdna
derivatives
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3220725A
Other languages
English (en)
French (fr)
Inventor
Pier Federico GHERARDINI
Tarun Kumar Khurana
Ali Agah
Yir-Shyuan WU
Filiz Gorpe YASAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellanome Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA3220725A1 publication Critical patent/CA3220725A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3220725A 2021-06-11 2022-06-10 Systems and methods for analyzing biological samples Pending CA3220725A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163209544P 2021-06-11 2021-06-11
US63/209,544 2021-06-11
PCT/US2022/033116 WO2022261507A1 (en) 2021-06-11 2022-06-10 Systems and methods for analyzing biological samples

Publications (1)

Publication Number Publication Date
CA3220725A1 true CA3220725A1 (en) 2022-12-15

Family

ID=84425364

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3220725A Pending CA3220725A1 (en) 2021-06-11 2022-06-10 Systems and methods for analyzing biological samples

Country Status (11)

Country Link
US (1) US20240301465A1 (https=)
EP (1) EP4352286A4 (https=)
JP (1) JP2024520804A (https=)
KR (1) KR20240019808A (https=)
CN (1) CN117813424A (https=)
AU (1) AU2022287983A1 (https=)
BR (1) BR112023025731A2 (https=)
CA (1) CA3220725A1 (https=)
IL (1) IL308886A (https=)
MX (1) MX2023014290A (https=)
WO (1) WO2022261507A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024253995A1 (en) * 2023-06-09 2024-12-12 Illumina, Inc. Methods of sequencing dna using flow cells containing multiple surfaces

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム, インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
CN120112795A (zh) * 2022-07-19 2025-06-06 赛拉诺姆公司 使用可降解水凝胶的单细胞蛋白质组学
WO2025128916A1 (en) 2023-12-13 2025-06-19 Cellanome, Inc. Devices and methods for analyzing biological samples
WO2025229401A2 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for screening of biological components
WO2025231451A1 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for analysis of cells

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5331476B2 (ja) * 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
WO2014060483A1 (en) * 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015065924A2 (en) * 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
SG11201609053YA (en) * 2014-04-29 2016-11-29 Illumina Inc Multiplexed single cell gene expression analysis using template switch and tagmentation
US10570447B2 (en) * 2014-05-16 2020-02-25 Illumina, Inc. Nucleic acid synthesis techniques
EP3350732B1 (en) * 2015-09-15 2024-07-24 Takara Bio USA, Inc. Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same
FI3662083T3 (fi) * 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
WO2019203728A1 (en) * 2018-04-20 2019-10-24 National University Of Singapore Linearization and labeling of single-stranded dna for optical sequence analysis
EP3844304B1 (en) * 2018-08-28 2024-10-02 10X Genomics, Inc. Methods for generating spatially barcoded arrays
WO2020190509A1 (en) * 2019-03-15 2020-09-24 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
EP4263859A1 (en) * 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024253995A1 (en) * 2023-06-09 2024-12-12 Illumina, Inc. Methods of sequencing dna using flow cells containing multiple surfaces

Also Published As

Publication number Publication date
AU2022287983A1 (en) 2023-12-14
CN117813424A (zh) 2024-04-02
US20240301465A1 (en) 2024-09-12
EP4352286A1 (en) 2024-04-17
WO2022261507A1 (en) 2022-12-15
JP2024520804A (ja) 2024-05-24
BR112023025731A2 (pt) 2024-02-27
KR20240019808A (ko) 2024-02-14
EP4352286A4 (en) 2025-04-23
IL308886A (en) 2024-01-01
MX2023014290A (es) 2024-02-27

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