CN117813424A - 用于分析生物样品的系统和方法 - Google Patents
用于分析生物样品的系统和方法 Download PDFInfo
- Publication number
- CN117813424A CN117813424A CN202280055747.XA CN202280055747A CN117813424A CN 117813424 A CN117813424 A CN 117813424A CN 202280055747 A CN202280055747 A CN 202280055747A CN 117813424 A CN117813424 A CN 117813424A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- molecules
- subset
- cdna
- derivatives
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163209544P | 2021-06-11 | 2021-06-11 | |
| US63/209,544 | 2021-06-11 | ||
| PCT/US2022/033116 WO2022261507A1 (en) | 2021-06-11 | 2022-06-10 | Systems and methods for analyzing biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117813424A true CN117813424A (zh) | 2024-04-02 |
Family
ID=84425364
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280055747.XA Pending CN117813424A (zh) | 2021-06-11 | 2022-06-10 | 用于分析生物样品的系统和方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20240301465A1 (https=) |
| EP (1) | EP4352286A4 (https=) |
| JP (1) | JP2024520804A (https=) |
| KR (1) | KR20240019808A (https=) |
| CN (1) | CN117813424A (https=) |
| AU (1) | AU2022287983A1 (https=) |
| BR (1) | BR112023025731A2 (https=) |
| CA (1) | CA3220725A1 (https=) |
| IL (1) | IL308886A (https=) |
| MX (1) | MX2023014290A (https=) |
| WO (1) | WO2022261507A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12576400B2 (en) | 2021-01-08 | 2026-03-17 | Cellanome, Inc. | Methods for incubating and analyzing a cell in a compartment of a fluidic device |
| JP2024502618A (ja) | 2021-01-08 | 2024-01-22 | セラノーム， インコーポレイテッド | 生体試料を分析するためのデバイスおよび方法 |
| CN120112795A (zh) * | 2022-07-19 | 2025-06-06 | 赛拉诺姆公司 | 使用可降解水凝胶的单细胞蛋白质组学 |
| WO2024253995A1 (en) * | 2023-06-09 | 2024-12-12 | Illumina, Inc. | Methods of sequencing dna using flow cells containing multiple surfaces |
| WO2025128916A1 (en) | 2023-12-13 | 2025-06-19 | Cellanome, Inc. | Devices and methods for analyzing biological samples |
| WO2025229401A2 (en) * | 2024-05-03 | 2025-11-06 | Cellanome, Inc. | Systems and methods for screening of biological components |
| WO2025231451A1 (en) * | 2024-05-03 | 2025-11-06 | Cellanome, Inc. | Systems and methods for analysis of cells |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5331476B2 (ja) * | 2005-06-15 | 2013-10-30 | カリダ・ジェノミックス・インコーポレイテッド | 遺伝子解析および化学解析用の単分子アレイ |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| WO2014060483A1 (en) * | 2012-10-17 | 2014-04-24 | Spatial Transcriptomics Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| WO2015065924A2 (en) * | 2013-10-28 | 2015-05-07 | Massachusetts Institute Of Technology | Hydrogel microstructures with immiscible fluid isolation for small reaction volumes |
| SG11201609053YA (en) * | 2014-04-29 | 2016-11-29 | Illumina Inc | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| US10570447B2 (en) * | 2014-05-16 | 2020-02-25 | Illumina, Inc. | Nucleic acid synthesis techniques |
| EP3350732B1 (en) * | 2015-09-15 | 2024-07-24 | Takara Bio USA, Inc. | Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same |
| FI3662083T3 (fi) * | 2017-08-01 | 2024-11-20 | Illumina Inc | Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä |
| WO2019203728A1 (en) * | 2018-04-20 | 2019-10-24 | National University Of Singapore | Linearization and labeling of single-stranded dna for optical sequence analysis |
| EP3844304B1 (en) * | 2018-08-28 | 2024-10-02 | 10X Genomics, Inc. | Methods for generating spatially barcoded arrays |
| WO2020190509A1 (en) * | 2019-03-15 | 2020-09-24 | 10X Genomics, Inc. | Methods for using spatial arrays for single cell sequencing |
| EP4263859A1 (en) * | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Single cell secretome analysis |
-
2022
- 2022-06-10 BR BR112023025731A patent/BR112023025731A2/pt unknown
- 2022-06-10 KR KR1020247000613A patent/KR20240019808A/ko active Pending
- 2022-06-10 WO PCT/US2022/033116 patent/WO2022261507A1/en not_active Ceased
- 2022-06-10 CN CN202280055747.XA patent/CN117813424A/zh active Pending
- 2022-06-10 MX MX2023014290A patent/MX2023014290A/es unknown
- 2022-06-10 EP EP22821163.7A patent/EP4352286A4/en active Pending
- 2022-06-10 AU AU2022287983A patent/AU2022287983A1/en active Pending
- 2022-06-10 IL IL308886A patent/IL308886A/en unknown
- 2022-06-10 CA CA3220725A patent/CA3220725A1/en active Pending
- 2022-06-10 JP JP2023575822A patent/JP2024520804A/ja active Pending
-
2023
- 2023-12-08 US US18/534,328 patent/US20240301465A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2022287983A1 (en) | 2023-12-14 |
| US20240301465A1 (en) | 2024-09-12 |
| EP4352286A1 (en) | 2024-04-17 |
| WO2022261507A1 (en) | 2022-12-15 |
| JP2024520804A (ja) | 2024-05-24 |
| BR112023025731A2 (pt) | 2024-02-27 |
| KR20240019808A (ko) | 2024-02-14 |
| EP4352286A4 (en) | 2025-04-23 |
| CA3220725A1 (en) | 2022-12-15 |
| IL308886A (en) | 2024-01-01 |
| MX2023014290A (es) | 2024-02-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |