JP2024520804A - 生体試料を分析するためのシステムおよび方法 - Google Patents

生体試料を分析するためのシステムおよび方法 Download PDF

Info

Publication number
JP2024520804A
JP2024520804A JP2023575822A JP2023575822A JP2024520804A JP 2024520804 A JP2024520804 A JP 2024520804A JP 2023575822 A JP2023575822 A JP 2023575822A JP 2023575822 A JP2023575822 A JP 2023575822A JP 2024520804 A JP2024520804 A JP 2024520804A
Authority
JP
Japan
Prior art keywords
nucleic acid
molecules
subset
cdna
derivatives
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023575822A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2022261507A5 (https=
JP2024520804A5 (https=
Inventor
ピエル フェデリコ ゲラルディーニ，
タルン クマール クラナ，
アリ アガー，
イル－シュアン ウー，
フィリズ ゴルぺ ヤサル，
Original Assignee
セラノーム， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セラノーム， インコーポレイテッド filed Critical セラノーム， インコーポレイテッド
Publication of JP2024520804A publication Critical patent/JP2024520804A/ja
Publication of JPWO2022261507A5 publication Critical patent/JPWO2022261507A5/ja
Publication of JP2024520804A5 publication Critical patent/JP2024520804A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2023575822A 2021-06-11 2022-06-10 生体試料を分析するためのシステムおよび方法 Pending JP2024520804A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163209544P 2021-06-11 2021-06-11
US63/209,544 2021-06-11
PCT/US2022/033116 WO2022261507A1 (en) 2021-06-11 2022-06-10 Systems and methods for analyzing biological samples

Publications (3)

Publication Number Publication Date
JP2024520804A true JP2024520804A (ja) 2024-05-24
JPWO2022261507A5 JPWO2022261507A5 (https=) 2025-06-18
JP2024520804A5 JP2024520804A5 (https=) 2025-06-18

Family

ID=84425364

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023575822A Pending JP2024520804A (ja) 2021-06-11 2022-06-10 生体試料を分析するためのシステムおよび方法

Country Status (11)

Country Link
US (1) US20240301465A1 (https=)
EP (1) EP4352286A4 (https=)
JP (1) JP2024520804A (https=)
KR (1) KR20240019808A (https=)
CN (1) CN117813424A (https=)
AU (1) AU2022287983A1 (https=)
BR (1) BR112023025731A2 (https=)
CA (1) CA3220725A1 (https=)
IL (1) IL308886A (https=)
MX (1) MX2023014290A (https=)
WO (1) WO2022261507A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム， インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
CN120112795A (zh) * 2022-07-19 2025-06-06 赛拉诺姆公司 使用可降解水凝胶的单细胞蛋白质组学
WO2024253995A1 (en) * 2023-06-09 2024-12-12 Illumina, Inc. Methods of sequencing dna using flow cells containing multiple surfaces
WO2025128916A1 (en) 2023-12-13 2025-06-19 Cellanome, Inc. Devices and methods for analyzing biological samples
WO2025229401A2 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for screening of biological components
WO2025231451A1 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for analysis of cells

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5331476B2 (ja) * 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
WO2014060483A1 (en) * 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015065924A2 (en) * 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
SG11201609053YA (en) * 2014-04-29 2016-11-29 Illumina Inc Multiplexed single cell gene expression analysis using template switch and tagmentation
US10570447B2 (en) * 2014-05-16 2020-02-25 Illumina, Inc. Nucleic acid synthesis techniques
EP3350732B1 (en) * 2015-09-15 2024-07-24 Takara Bio USA, Inc. Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same
FI3662083T3 (fi) * 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
WO2019203728A1 (en) * 2018-04-20 2019-10-24 National University Of Singapore Linearization and labeling of single-stranded dna for optical sequence analysis
EP3844304B1 (en) * 2018-08-28 2024-10-02 10X Genomics, Inc. Methods for generating spatially barcoded arrays
WO2020190509A1 (en) * 2019-03-15 2020-09-24 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
EP4263859A1 (en) * 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis

Also Published As

Publication number Publication date
AU2022287983A1 (en) 2023-12-14
CN117813424A (zh) 2024-04-02
US20240301465A1 (en) 2024-09-12
EP4352286A1 (en) 2024-04-17
WO2022261507A1 (en) 2022-12-15
BR112023025731A2 (pt) 2024-02-27
KR20240019808A (ko) 2024-02-14
EP4352286A4 (en) 2025-04-23
CA3220725A1 (en) 2022-12-15
IL308886A (en) 2024-01-01
MX2023014290A (es) 2024-02-27

Similar Documents

Publication Publication Date Title
US20240301465A1 (en) Systems and methods for analyzing biological samples
US11873530B1 (en) Systems and methods for metabolome analysis
US12606860B2 (en) Systems and methods for processing RNA from cells
US11155810B2 (en) Single cell analysis of transposase accessible chromatin
KR102852123B1 (ko) Dna 가공을 위한 중합체 비드의 조절
CN105492607B (zh) 用于样品处理的组合物和方法
CN107427808B (zh) 用于制备核酸测序文库的方法和系统以及用其制备的文库
CN115916972A (zh) 用于被固定样品的组合物和方法
CN114616341A (zh) 联接介导的核酸分析
CN113874521A (zh) 用于富集条形码的方法和系统
JP7773373B2 (ja) オンフローセル三次元ポリマー構造
CN119842876A (zh) 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备
US20250050301A1 (en) Spatial barcodes by hydrogel lithography
EP4496898A1 (en) Multiplex cellular assays for screening and quality assessment of engineered cells
WO2024145393A1 (en) Spatially barcoded surfaces
RU2822154C2 (ru) Модуляция полимерных гранул для обработки днк
CN117015615A (zh) 用于分析生物样品的装置和方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250610

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20250610