KR20240009012A - Composition for preventing hair loss or promoting hair growth comprising yeast extract cultured by applying oxidative stress - Google Patents

Abstract

The present disclosure discloses a composition for preventing hair loss or promoting hair growth comprising a yeast extract cultured by applying oxidative stress. The composition includes a Saccharomyces cerevisiae strain, a culture of the strain, a lysate of the strain, an extract of the strain, or an extract of the culture or lysate as an active ingredient. The composition is effective in preventing hair loss or promoting hair growth by promoting the growth of dermal papilla cells.

Description

Composition for preventing hair loss or promoting hair growth comprising yeast extract cultured by applying oxidative stress {COMPOSITION FOR PREVENTING HAIR LOSS OR PROMOTING HAIR GROWTH COMPRISING YEAST EXTRACT CULTURED BY APPLYING OXIDATIVE STRESS}

The present disclosure discloses a composition for preventing hair loss or promoting hair growth comprising a yeast extract cultured by applying oxidative stress.

The cause of hair loss has not yet been clearly identified, but it is known to be largely due to genetic factors and is also caused by poor blood circulation in the scalp, excessive action of male hormones, excessive sebum secretion, aging, and stress. Various types of hair loss prevention and hair growth agents are being developed according to these various causes, but the actual hair loss prevention and hair growth effects are minimal.

Meanwhile, as chemical and animal raw materials have recently been avoided, much research is being conducted to find eco-friendly materials. Microbial resources are classified as sustainable resources in that they can be reproduced, unlike oil and water, and have high potential for research and industrial application because they utilize the unique characteristics of microorganisms that have adapted to various environments. Accordingly, efforts are being made to develop compositions that exhibit excellent hair loss prevention and/or hair growth promotion effects by utilizing active ingredients derived from microorganisms.

KR 10-2018-0085667 A

In one aspect, the purpose of the present disclosure is to provide a composition for preventing hair loss or promoting hair growth.

In another aspect, the purpose of the present disclosure is to provide a method for producing the composition for preventing hair loss or promoting hair growth.

In one aspect, the present disclosure is a method for preventing hair loss or hair growth comprising a Saccharomyces cerevisiae strain, a culture of the strain, a lysate of the strain, an extract of the strain, or an extract of the culture or lysate as an active ingredient. A composition for promotion is provided.

In an exemplary embodiment, the Saccharomyces cerevisiae strain may be Saccharomyces cerevisiae strain JTL-L1.

In an exemplary embodiment, the Saccharomyces cerevisiae strain may be cultured under conditions where oxidative stress is applied.

In an exemplary embodiment, the conditions for applying the oxidative stress include hydrogen peroxide (H ₂ O ₂ ), superoxide ion (0 ₂ ^- ), hydroxyl radical (OH ^- ), and hypochlorous acid in the culture medium. (hypochlorous acid, HOCl) may be added.

In an exemplary embodiment, the conditions for applying the oxidative stress include adding 0.001 v/v% to 0.03 v/v% of at least one selected from the group consisting of hydrogen peroxide, superoxide ion, hydroxyl radical, and hypochlorous acid to the culture medium. It may have been added at different concentrations.

In an exemplary embodiment, the active ingredient may be an extract of Saccharomyces cerevisiae strain.

In an exemplary embodiment, the extract may be extracted with one or more extraction solvents selected from water and lower alcohols having 1 to 4 carbon atoms.

In an exemplary embodiment, the extract may be extracted by adding an extraction solvent at a concentration of 1 v/v% to 50 v/v%.

In one exemplary embodiment, the active ingredient may promote the growth of dermal papilla cells.

In one exemplary embodiment, the active ingredient may be included in an amount of 0.0025% by weight or more based on the total weight of the composition.

In another aspect, the present disclosure is a method of preparing the composition for preventing hair loss or promoting hair growth, comprising culturing a Saccharomyces cerevisiae strain by applying oxidative stress. A composition for preventing hair loss or promoting hair growth. Provides a method for manufacturing.

In one aspect, the technology disclosed in the present disclosure has the effect of providing a composition for preventing hair loss or promoting hair growth.

In another aspect, the technology disclosed in the present disclosure has the effect of providing a method for producing the composition for preventing hair loss or promoting hair growth.

Figure 1 shows the sequence of the yeast ITS region isolated and identified according to one example.
Figure 2 shows the results of culturing various yeast strains under oxidative stress conditions according to one embodiment.
Figure 3 shows the results of comparing the antioxidant activity of the yeast strain after culturing it under general culture conditions and oxidative stress culture conditions according to an example (*: p < 0.05, t-test, n=3 , Difference in antioxidant power as a result of oxidative stress culture compared to normal culture of JTL-L1).
Figure 4 shows the results confirming the dermal papilla cell proliferation efficacy of Saccharomyces cerevisiae strain JTL-L1 cultured under oxidative stress culture conditions according to an embodiment (*: p < 0.05, **: p < 0.005, ***: p < 0.001, t-test, n=3, survival rate of dermal papilla cells in the JTL-L1 extract treated group cultured under oxidative stress compared to the untreated group).
Figure 5 shows the results of comparing the dermal papilla cell proliferation efficacy of the yeast strain after culturing it under general culture conditions and oxidative stress culture conditions according to an embodiment (*: p < 0.05, t-test test, n =3, survival rate of dermal papilla cells compared to untreated group; and #: p < 0.05, t-test test, n=3, difference in survival rate of dermal papilla cells as a result of oxidative stress culture compared to normal culture of JTL-L1).

Hereinafter, the present disclosure will be described in detail.

In one aspect, the present disclosure is a method for preventing hair loss or hair growth comprising a Saccharomyces cerevisiae strain, a culture of the strain, a lysate of the strain, an extract of the strain, or an extract of the culture or lysate as an active ingredient. A composition for promotion is provided.

In another aspect, the present disclosure is a method of preparing the composition for preventing hair loss or promoting hair growth, comprising culturing a Saccharomyces cerevisiae strain by applying oxidative stress. A composition for preventing hair loss or promoting hair growth. Provides a method for manufacturing.

The hair cycle can be divided into three main stages known as the anagen phase, catagen phase, and telogen phase. During the growth phase, hair is formed as hair follicles grow deeper into the skin along with rapid proliferation of cells. The next phenomenon is the catagen phase, which is a transitional phase in which the cessation of cell division is noticeable. During this process, the hair follicles gradually degenerate and hair growth stops. In the next phase, the telogen phase, the degenerating hair follicle contains a germ containing densely packed dermal papilla cells. The initiation of new anagen events in the telogen phase is induced by rapid cell proliferation in the embryo, expansion of the dermal papilla, and synthesis of basement membrane elements. Generally, hair growth occurs in the anagen phase and is promoted by inducing the telogen phase to the growth phase and delaying the growth phase to the catagen phase.

As used herein, hair loss refers to the phenomenon of hair falling out from the scalp or hair becoming sparse or thin, hair loss prevention refers to preventing and suppressing the hair loss phenomenon described above, and hair growth promotion refers to the growth of new hair. It refers not only to the function of producing hair growth, or the function of promoting hair growth, but also the function of promoting the delay from the growth phase to the catagen phase and allowing existing hair to grow healthily.

Saccharomyces cerevisiae is one of the yeasts and has been used for making makgeolli, beer, wine, and baking since ancient times. It is a eukaryotic organism like humans and is a biological model often used in molecular biology or cell biology.

In an exemplary embodiment, the Saccharomyces cerevisiae strain may be Saccharomyces cerevisiae strain JTL-L1.

In an exemplary embodiment, the Saccharomyces cerevisiae strain may be cultured under conditions where oxidative stress is applied.

As used herein, oxidative stress refers to stress that may damage cells or tissues due to the presence of oxidative substances or active oxygen in the surrounding environment of cells.

In an exemplary embodiment, the conditions for applying the oxidative stress include hydrogen peroxide (H ₂ O ₂ ), superoxide ion (0 ₂ ^- ), hydroxyl radical (OH ^- ), and hypochlorous acid in the culture medium. (hypochlorous acid, HOCl) may be added.

In an exemplary embodiment, the condition for applying the oxidative stress may be adding hydrogen peroxide to the culture medium.

In an exemplary embodiment, the conditions for applying the oxidative stress include adding at least one selected from the group consisting of hydrogen peroxide, superoxide ion, hydroxyl radical, and hypochlorous acid to the culture medium at a concentration of 0.001% or more, 0.001 v/v% to 0.03 Add at a concentration of v/v%, a concentration of 0.005 v/v% to 0.03 v/v%, a concentration of 0.01 v/v% to 0.03 v/v%, or a concentration of 0.01 v/v% to 0.02 v/v%. It may have been done.

In an exemplary embodiment, the conditions for applying the oxidative stress include adding hydrogen peroxide to the culture medium at a concentration of 0.001% or more, a concentration of 0.001 v/v% to 0.03 v/v%, or 0.005 v/v% to 0.03 v/v%. It may be added at a concentration of 0.01 v/v% to 0.03 v/v%, or 0.01 v/v% to 0.02 v/v%.

In an exemplary embodiment, the active ingredient may be an extract of Saccharomyces cerevisiae strain.

In an exemplary embodiment, the extract may be extracted with one or more extraction solvents selected from water and lower alcohols having 1 to 4 carbon atoms.

In one exemplary embodiment, the extract may be an ethanol extract.

In an exemplary embodiment, the extract may be extracted by adding an extraction solvent at a concentration of 1 v/v% to 50 v/v%. In another exemplary embodiment, the extract contains at least 1 v/v%, at least 5 v/v%, at least 10 v/v%, at least 15 v/v%, or at least 20 v/v%, Concentration of 50 v/v% or less, 45 v/v% or less, 40 v/v% or less, 35 v/v% or less, 30 v/v% or less, 25 v/v% or less, or 20 v/v% or less It may have been extracted by adding .

In an exemplary embodiment, the extract may be extracted by adding an aqueous ethanol solution at a concentration of 1 v/v% to 50 v/v%. In another exemplary embodiment, the extract contains at least 1 v/v%, at least 5 v/v%, at least 10 v/v%, at least 15 v/v%, or at least 20 v/v% of an ethanol aqueous solution, Concentration of 50 v/v% or less, 45 v/v% or less, 40 v/v% or less, 35 v/v% or less, 30 v/v% or less, 25 v/v% or less, or 20 v/v% or less It may have been extracted by adding .

In one exemplary embodiment, the active ingredient may promote the growth of dermal papilla cells. Hair follicle dermal papilla cells play an important role in hair growth and cycle regulation. The composition according to the present disclosure provides the effect of preventing, improving, and/or treating hair loss and promoting hair growth or hair growth by promoting the expression of vascular endothelial growth factor in dermal papilla cells.

In an exemplary embodiment, the active ingredient may be included in an amount of 0.0001% by weight or more, 0.0025% by weight or more, or 0.0001 to 20% by weight based on the total weight of the composition.

In one exemplary embodiment, the composition may be a pharmaceutical composition for preventing or treating hair loss.

In addition to the active ingredients, the pharmaceutical composition may further contain pharmaceutical auxiliaries such as preservatives, stabilizers, hydrating agents or emulsification accelerators, salts and/or buffering agents for osmotic pressure adjustment, and other therapeutically useful substances, and may be used in a conventional manner. Accordingly, it can be formulated into various oral or parenteral dosage forms.

The oral administration agents include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, powders, powders, fine granules, granules, pellets, etc., and these formulations contain surfactants in addition to the active ingredients. , may contain diluents (e.g. lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (e.g. silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycol). . Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, and in some cases starch, agar, alginic acid or its sodium salt. It may contain pharmaceutical additives such as disintegrants, absorbents, colorants, flavoring agents, and sweeteners. The tablets can be prepared by conventional mixing, granulating or coating methods.

In addition, the parenteral dosage form may be a transdermal dosage form, for example, injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, patches, etc. It may be, but is not limited to this.

The determination of the dosage of the active ingredient is within the level of a person skilled in the art, and the daily dosage of the drug varies depending on various factors such as the degree of progression of the subject to be administered, time of onset, age, health condition, and complications, but is not recommended for adults. Based on, in one aspect, 1 μg/kg to 200 mg/kg of the composition, and in another aspect, 50 μg/kg to 50 mg/kg can be administered in divided doses 1 to 3 times a day, and the dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition may be an external preparation for the skin, and the external preparation for the skin is a general term that can include anything applied outside the skin, and various dosage forms of medicines may be included here.

In one exemplary embodiment, the composition may be a cosmetic composition for suppressing hair loss or promoting hair growth.

In addition to the active ingredients, the cosmetic composition may further include functional additives and components included in general cosmetic compositions. The functional additive may include ingredients selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohol, pigments, fragrances, and blood circulation agents. Examples include accelerators, cooling agents, restrictors, and purified water.

The formulation of the cosmetic composition is not particularly limited and can be appropriately selected depending on the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, pack, soap, cleansing. It may be manufactured in one or more formulations selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion, and body cleanser, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier component. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

In one exemplary embodiment, the composition may be a food composition for inhibiting hair loss or promoting hair growth.

The food composition may be in a liquid or solid form, for example, various foods, beverages, gums, teas, vitamin complexes, health supplements, etc., and may be used in the form of powders, granules, tablets, capsules, or beverages. You can. In addition to the active ingredients, the food composition of each formulation can be appropriately selected and mixed by a person skilled in the art according to the formulation or purpose of use, without difficulty, and a synergistic effect can occur when applied simultaneously with other raw materials.

There are no particular restrictions on the liquid ingredients that can be contained in addition to the active ingredients disclosed in this specification, and, like regular beverages, various flavoring agents or natural carbohydrates can be included as additional ingredients. Examples of the natural carbohydrates include monosaccharides, disaccharides such as glucose and fructose, polysaccharides such as maltose and sucrose, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. etc. As the above flavoring agent, natural flavoring agents (thaumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (e.g., saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate may be generally about 1 to 20 g, and in one aspect, about 5 to 12 g per 100 ml of the composition disclosed herein.

In one aspect, the food composition includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its It may include salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In another aspect, it may include pulp for the production of natural fruit juices and vegetable drinks. The above ingredients can be used independently or in combination. The ratio of the additive may vary, but is generally selected in the range of 0.001 to about 20 parts by weight per 100 parts by weight of the composition disclosed herein.

Hereinafter, the present disclosure will be described in more detail through examples. It will be apparent to those skilled in the art that these examples are solely for illustrating the present disclosure, and that the scope of the present disclosure should not be construed as limited by these examples.

Example 1. Isolation and identification of yeast

Saccharomyces cerevisiae was isolated from fermented product obtained in the process of recreating Gangsul, a traditional liquor from Jeju Island. Gangsul is a traditional liquor in the Jeju region. Unlike on the mainland, Gangsul is a semi-solid liquor made by mixing malt with rice cake made from millet and fermenting it without adding water. Currently, only the manufacturing method remains and is not for sale.

Jeju Island's traditional perilla perilla was washed several times under running water, dried with hot air (60°C, over 1 hour), ground, and sieved to prepare perilla powder. The prepared perilla powder was kneaded with boiled water to make round Omegi rice cakes, and then boiled until the rice cakes floated in water. Before the boiled omegi rice cake was completely cooled, it was mixed with yeast at a weight ratio of about 3:1, then transferred to a traditional Jeju Island pottery and fermented at 17-20 ℃.

According to the recipe of Gangsul, 1 g of the result of fermentation for 45 days was diluted and spread on YPD agar medium to obtain colonies of the strain, and subcultured several times until a single colony was identified. DNA was extracted from the single colony thus obtained, and the sequence of the ITS (Internal Transcribed Spacer) region was confirmed to confirm that the isolated strain was Saccharomyces cerevisiae . The sequence of the ITS region is shown in Figure 1. The identified strain was named Saccharomyces cerevisiae JTL-L1 and deposited on April 4, 2017 at the Korea Center for Microbial Conservation, a depository organization, under the deposit number KCCM12008P.

Example 2. Cultivation of yeast under oxidative stress conditions

Saccharomyces cerevisiae strain JTL-L1 isolated and identified in Example 1, a standard yeast strain isolated from wine ( S. cerevisiae KCTC17798), and a commercially available beer-making yeast strain ( S. cerevisiae safale US -05), a yeast strain ( S. cerevisiae APmicroorganisms library No.905, FKN-PDA1) that the company directly isolated from koji, and a yeast strain ( Galactomyces candidus KCCM60351) used in making sake under culture conditions with oxidative stress. The growth of yeast was compared by culturing. The yeast strain that the company directly isolated from the yeast was obtained by obtaining yeast powder from a traditional market in the Seongsan area of Jeju Island, diluting 1 g of yeast powder in PBS (phosphate buffer saline), and spreading it on PD agar (potato dextrose agar) medium. and subcultured until a single colony was identified. DNA was extracted from the single colony, and the sequence of the ITS region was confirmed to confirm that the isolated strain was Saccharomyces cerevisiae .

Specifically, according to the general method, each strain cultured in YPD medium (yeast extract 10 g/L, peptone 20 g/L, glucose 20 g/L) was subcultured to new YPD medium while adding 0.01% and 0.02% hydrogen peroxide. It was added in concentration (v/v). After culturing with shaking at 120 rpm at 25 to 30° C. for 24 hours, the growth of the strains was compared by measuring the absorbance of the culture at 600 nm.

As a result, as shown in Figure 2, the standard strain of Saccharomyces cerevisiae, the beer-making yeast strain, and the Galactomyces yeast strain showed little growth, and the JTL-L1 strain and yeast yeast according to the present disclosure Only the strain was able to maintain growth. Through this, it was confirmed that not all yeast or Saccharomyces cerevisiae can be cultured under conditions where oxidative stress is applied, and that there are differences in the degree to which oxidative stress is tolerated depending on the strain.

Example 3. Comparison of antioxidant efficacy

The antioxidant activity of the Saccharomyces cerevisiae JTL-L1 strain, which was confirmed to be capable of growing under oxidative stress conditions in Example 2, and the yeast strain isolated from yeast were compared and evaluated according to culture conditions.

First, the JTL-L1 strain and the nuruk yeast strain were mixed with 10 ml of medium containing hydrogen peroxide at a concentration of 0.02% in general YPD medium (yeast extract 10 g/L, peptone 20 g/L, glucose 20 g/L) and hydrogen peroxide. Each was inoculated into 10 ml of unadded medium and cultured with shaking at 120 rpm for 2 days at 25 to 30°C. The culture was centrifuged (4000 rpm, 10 minutes), the culture supernatant was discarded, and the yeast cells were harvested. The harvested cells were washed again in 10 ml of sterilized water, the washing liquid was removed by centrifugation, and 10 ml of a 20% (v/v) ethanol solution was poured into the remaining cells and extracted at 50°C for 2 hours. The ethanol extract of yeast was dried through reduced pressure concentration, diluted in sterilized water to the same concentration, and used in the experiment.

The antioxidant efficacy of extracts (10 mg/mL) of JTL-L1 strain and Nuruk yeast strain with and without oxidative stress was measured using the ABTS test method. The ABTS test method is a method of calculating antioxidant level by measuring the ability to remove ABTS radical cations induced by metmyoglobin and hydrogen peroxide. Trolox standard was prepared at an appropriate concentration, and myoglobin working solution, a reaction reagent, was prepared. 10 μL of sample or trolox standard was dispensed into a 96 well plate, and assay buffer was dispensed into a negative control well. After adding 20 μL of myoglobin working solution to each well, 100 μL of ABTS solution was dispensed per well and reacted at room temperature for 5 minutes. Absorbance was measured at 405 nm and compared with the trolox standard, and the antioxidant activity of each sample was expressed in Trolox equivalents (TE, μM).

As a result of checking whether there was any difference between the yeast strain cultured under conditions of applying oxidative stress by adding hydrogen peroxide as described above and the yeast strain cultured under normal culture conditions, as shown in Figure 3, the JTL-L1 strain was cultured under normal culture conditions. Antioxidant activity was significantly increased when cultured under oxidative stress conditions, whereas yeast yeast strains, like the JTL-L1 strain, can grow under oxidative stress conditions, but did not show an increase in antioxidant activity compared to normal culture conditions. . Therefore, it was confirmed that even though growth was possible under conditions of oxidative stress, there was a difference in antioxidant activity depending on the strain.

Example 4. Dermal papilla cell proliferation efficacy of Saccharomyces cerevisiae strain JTL-L1

In order to confirm the effect of the extract of Saccharomyces cerevisiae strain JTL-L1 cultured under conditions of applying oxidative stress obtained in Example 3 above on the growth of human dermal papilla cells, human dermal papilla cells were treated with the extract. After this, the cell viability was confirmed.

Specifically, human dermal papilla cells were grown in 96 wells at 2×10 ² cells/100 μL per well using DMEM (Dulbecco's Modified eagle media) medium containing 10% FBS (fetal bovine serum) and 1% antibiotics. It was dispensed onto a plate and cultured at 37°C for 72 hours under 5% CO ₂ humidified conditions. After 24 hours, the medium used for culture was removed from each well of the 96-well plate, washed twice by adding PBS (phosphate buffered saline), and then DMEM medium without FBS was added.

Thereafter, the yeast strain JTL-L1 extract was added to a 96-well plate at a concentration of 25 to 100 ppm, cultured for 72 hours, and cell viability (cell counting kit-8) was measured using CCK-8 reagent (cell counting kit-8). viability) was measured. The degree of proliferation was compared as a percentage based on untreated wells.

As a result, as shown in Figure 4, the extract of Saccharomyces cerevisiae strain JTL-L1 cultured under oxidative stress conditions significantly promoted the growth of dermal papilla cells to prevent hair loss and/or promote hair growth. It was confirmed that it was effective.

Example 5. Comparison of dermal papilla cell proliferation efficacy

Comparative evaluation of the dermal papilla cell proliferation efficacy of the Saccharomyces cerevisiae JTL-L1 strain, which was confirmed to be capable of growing under oxidative stress conditions in Example 2, and the yeast strain isolated from yeast, according to culture conditions. did.

First, the JTL-L1 strain and the nuruk yeast strain were mixed with 10 ml of medium containing hydrogen peroxide at a concentration of 0.02% in general YPD medium (yeast extract 10 g/L, peptone 20 g/L, glucose 20 g/L) and hydrogen peroxide. Each was inoculated into 10 ml of unadded medium and cultured with shaking at 120 rpm for 2 days at 25 to 30°C. The culture was centrifuged (4000 rpm, 10 minutes), the culture supernatant was discarded, and the yeast cells were harvested. The harvested cells were washed again in 10 ml of sterilized water, the washing liquid was removed by centrifugation, and 10 ml of a 20% (v/v) ethanol solution was poured into the remaining cells and extracted at 50°C for 2 hours. The ethanol extract of yeast was dried through reduced pressure concentration, diluted in sterilized water to the same concentration, and used in the experiment.

The efficacy of dermal papilla cell proliferation was evaluated in the same manner as in Example 4, but as a result of treating each yeast extract at a concentration of 100 ppm, the effect of inducing proliferation in dermal papilla cells was different even if the yeast extract was of the same type, and Saka In the case of Romayces cerevisiae strain JTL-L1, it was confirmed that the proliferation of dermal papilla cells could be further increased when cultured by applying oxidative stress compared to normal culture (see Figure 5).

A formulation example of a composition according to one aspect of the present disclosure is described below, but various other formulations can also be applied, and this is not intended to limit the present disclosure, but is only intended to provide a detailed description.

[Formulation Example 1] Soft capsule formulation

Cultivation under oxidative stress conditions in Example 3 50 mg of Saccharomyces cerevisiae strain JTL-L1 extract, 80 to 140 mg of L-carnitine, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of hydrogenated vegetable oil, 4 mg of yellow lead, and 6 mg of lecithin were mixed, and usually Soft capsules were prepared by filling 400 mg per capsule according to the method.

[Formulation Example 2] Tablets

Cultivation under oxidative stress conditions in Example 3: 50 mg of Saccharomyces cerevisiae strain JTL-L1 extract, 200 mg of galactooligosaccharide, 60 mg of lactose, and 140 mg of maltose were mixed and granulated using a fluidized bed dryer, followed by sugar ester. Tablets were prepared by adding 6 mg and compressing it with a tablet press.

[Formulation Example 3] Granules

Cultured under oxidative stress conditions in Example 3 50 mg of Saccharomyces cerevisiae strain JTL-L1 extract, 250 mg of anhydrous crystalline glucose, and 550 mg of starch were mixed, formed into granules using a fluidized bed granulator, and then filled into bags to form granules. Manufactured.

[Formulation example 4] Drink formulation

Culture under oxidative stress conditions in Example 3 50 mg of Saccharomyces cerevisiae strain JTL-L1 extract, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide were mixed, and then 300 ml of purified water was added, and 200 ml of each bottle was filled. After filling the bottle, it was sterilized at 130°C for 4 to 5 seconds to prepare a drinkable beverage.

[Formulation Example 5] Ointment

An ointment was prepared by a conventional method according to the composition shown in Table 1 below.

ingredient Content (% by weight) Saccharomyces cerevisiae strain JTL-L1 extract cultured under oxidative stress conditions in Example 3 2.00 glycerin 8.00 Butylene glycol 4.00 liquid paraffin 15.00 beta glucan 7.00 carbomer 0.10 Caprylic/Capric Triglyceride 3.00 squalane 1.00 Cetearyl Glucoside 1.50 Sorbitan Stearate 0.40 cetearyl alcohol 1.00 beeswax 4.00 Purified water remaining amount Sum 100.00

[Formulation Example 6] Cosmetic liquid formulation

A cosmetic liquid formulation was prepared by a conventional method according to the composition shown in Table 2 below.

ingredient Content (% by weight) Saccharomyces cerevisiae strain JTL-L1 extract cultured under oxidative stress conditions in Example 3 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 dark glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00 Purified water remaining amount Sum 100.00

[Formulation Example 7] Shampoo

Shampoo was prepared by a conventional method according to the composition shown in Table 3 below.

ingredient Content (% by weight) Saccharomyces cerevisiae strain JTL-L1 extract cultured under oxidative stress conditions in Example 3 2.00 Sodium lauryl sulfate 10.00 Cocamidopropyl Betaine 3.00 Carboxyl vinyl polymer 0.30 Polyquaternium-10 0.20 Cetyltrimethylammonium chloride 0.10 Purified water remaining amount Sum 100.00

[Formulation Example 8] Rinse

A rinse was prepared by a conventional method according to the composition shown in Table 4 below.

ingredient Content (% by weight) Saccharomyces cerevisiae strain JTL-L1 extract cultured under oxidative stress conditions in Example 3 2.00 cetyl alcohol 2.00 stearyl alcohol 2.50 Behenyl alcohol 0.50 silicone emulsion 0.40 cyclomethicone 1.00 Dimethyldistearylammonium chloride 0.10 Purified water remaining amount Sum 100.00

As described above, specific parts of the present disclosure have been described in detail, and it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present disclosure. something to do. Accordingly, the substantial scope of the present disclosure will be defined by the appended claims and their equivalents.

Korea Microbial Conservation Center KCCM12008P 20170404

Claims (11)

A composition for preventing hair loss or promoting hair growth comprising a Saccharomyces cerevisiae strain, a culture of the strain, a lysate of the strain, an extract of the strain, or an extract of the culture or lysate as an active ingredient.
According to clause 1,
The composition for preventing hair loss or promoting hair growth, wherein the Saccharomyces cerevisiae strain is Saccharomyces cerevisiae strain JTL-L1.
According to clause 1,
A composition for preventing hair loss or promoting hair growth, wherein the Saccharomyces cerevisiae strain is cultured under conditions under which oxidative stress is applied.
According to clause 3,
The conditions under which the oxidative stress was applied were hydrogen peroxide (H ₂ O ₂ ), superoxide ion (0 ₂ ^- ), hydroxyl radical (OH ^- ), and hypochlorous acid (HOCl) in the culture medium. A composition for preventing hair loss or promoting hair growth, to which one or more selected from the group consisting of is added.
According to clause 3,
The conditions for applying the oxidative stress include adding at least one selected from the group consisting of hydrogen peroxide, superoxide ion, hydroxyl radical, and hypochlorous acid to the culture medium at a concentration of 0.001 v/v% to 0.03 v/v%, A composition for preventing hair loss or promoting hair growth.
According to clause 1,
A composition for preventing hair loss or promoting hair growth, wherein the active ingredient is an extract of Saccharomyces cerevisiae strain.
According to clause 6,
A composition for preventing hair loss or promoting hair growth, wherein the extract is extracted with at least one extraction solvent selected from water and lower alcohols having 1 to 4 carbon atoms.
According to clause 6,
The extract is a composition for preventing hair loss or promoting hair growth, which is extracted by adding an extraction solvent at a concentration of 1 v/v% to 50 v/v%.
According to clause 1,
A composition for preventing hair loss or promoting hair growth, wherein the active ingredient promotes the growth of dermal papilla cells.
According to clause 1,
A composition for preventing hair loss or promoting hair growth, wherein the active ingredient is contained in an amount of 0.0025% by weight or more based on the total weight of the composition.
A method for producing the composition for preventing hair loss or promoting hair growth according to any one of claims 1 to 10,
A method of producing a composition for preventing hair loss or promoting hair growth, comprising culturing a Saccharomyces cerevisiae strain by applying oxidative stress.