KR20230173287A - A cosmetic composition comprising rucinol and natural extracts for skin-whitening - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, and provides a cosmetic composition containing rucinol and acacia extract as active ingredients.

Description

Cosmetic composition for skin whitening containing rucinol and natural extracts {A COSMETIC COMPOSITION COMPRISING RUCINOL AND NATURAL EXTRACTS FOR SKIN-WHITENING}

The present invention relates to a multifunctional cosmetic composition with less skin irritation and significantly improved skin whitening effect.

The skin is the most basic defense organ that protects the body from irritation in contact with the external environment, and is an important organ that expresses external beauty.

Because external beauty is not only a criterion for judgment, symbolizing youth and health, but also a competitive advantage that expresses confidence and affects work ability, the tendency to take care of one's skin is increasing in modern society.

As the industry develops, environmental pollution becomes more serious, and interest in anti-aging and whitening of the skin is increasing.

In particular, human skin constantly undergoes changes, the most representative changes being the decline in skin function and visual beauty due to aging.

Final skin appearance phenomena that occur as a result of skin aging include the formation of wrinkles, decreased skin elasticity, and formation of senile spots, with the formation of wrinkles being considered the most representative phenomenon.

Lipids, proteins, polysaccharides, and nucleic acids, which are the main components of skin, can be oxidized, destroying skin cells and tissues, and ultimately causing skin aging.

In particular, due to protein oxidation, the connective tissue of the skin, such as collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, may be cut and an excessive inflammatory response may occur. In further cases, mutations due to DNA mutation and a decrease in immune function may occur. , the cause of cancer may be promoted.

Therefore, it is important to protect cell membranes by scavenging reactive oxygen species generated during the body's metabolic process, ultraviolet irradiation, and inflammatory reactions, and to restore skin health by regenerating damaged cells through active metabolism. desirable.

Meanwhile, melanin refers to a black or brown pigment present in skin, hair, etc. Melanin exists in the skin and has the function of protecting the skin by blocking ultraviolet rays and maintaining the skin's body temperature.

Melanin is produced by converting tyrosine into dopa and dopaquinone through the action of tyrosinase present in pigment cells and passing through dopachrome.

Melanin protects the body from harmful skin elements such as ultraviolet rays and regulates hormone secretion in the body.

However, melanin is overproduced and can promote the formation of spots and freckles and skin aging, and can be involved in causing skin cancer, so research and development is being actively conducted to suppress the overproduction of melanin.

Recently, the cosmetics industry has been developing a number of products using natural products to reduce skin irritation caused by various chemicals.

Not only do natural ingredients have fewer side effects on the skin, but their development value as cosmetic raw materials is gradually increasing as consumers' response to cosmetics using natural ingredients has recently increased.

As such, the present inventors attempted to develop a cosmetic composition with excellent functionality using natural extracts in a situation where the need for developing cosmetic products derived from natural products with excellent biosafety and skin improvement effects is constantly emerging.

(Patent Document 1) Republic of Korea Patent No. 10-2014842

(Patent Document 2) Republic of Korea Patent No. 10-2336538

The present invention is intended to solve the above-mentioned problems, and the purpose of the present invention is to identify excellent synergistic activity through raw materials with proven skin safety, and thereby provide a cosmetic composition with excellent functionality and biosafety.

According to one aspect of the present invention, there is provided a cosmetic composition for skin whitening containing rucinol and Camagsari extract as active ingredients.

In one embodiment, the cosmetic composition may further include euri extract.

In one embodiment, the cosmetic composition may further include buckwheat flower extract.

In one embodiment, the cosmetic composition may further include one or more of Baekhyangaceae extract and Jilryeoja extract.

In one embodiment, the cosmetic composition may further include antioxidant properties.

In one embodiment, the cosmetic composition may further include a use for improving wrinkles or preventing photoaging.

In one embodiment, the cosmetic composition includes softening lotion, nourishing lotion, moisturizing cream, nourishing cream, massage cream, nourishing lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, It may be formulated with one or more selected from the group consisting of mask pack, spray, and powder.

According to another aspect of the present invention, there is provided an external composition for skin whitening containing rucinol and Camagsari extract as active ingredients.

According to the present invention, the cosmetic composition composition can minimize the content of chemical substances that can cause skin irritation compared to natural ingredients.

The skin whitening effect of the cosmetic composition can be significantly improved through the synergistic activity of previously known compounds and natural ingredients.

The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

The terms used in this specification are general terms that are currently widely used as much as possible while considering the function in the present invention, but this may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, etc. In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the relevant invention. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than simply the name of the term.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless clearly defined in the present application, should not be interpreted in an ideal or excessively formal sense. No.

The numerical range includes the values defined in the range above. Every maximum numerical limit given throughout this specification includes all lower numerical limits as if the lower numerical limit were explicitly written out. Every minimum numerical limit given throughout this specification includes every higher numerical limit as if such higher numerical limit was clearly written. All numerical limits given throughout this specification will include all better numerical ranges within the broader numerical range, as if the narrower numerical limits were clearly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited to the following examples.

One aspect of the present invention provides a cosmetic composition for skin whitening containing rucinol and a cauliflower extract as active ingredients, and another aspect of the present invention provides a cosmetic composition for skin whitening containing rucinol and a cauliflower extract as active ingredients. Provided is a whitening external composition.

The cosmetic composition can realize excellent skin functionality, including skin whitening, through the synergistic activity of various complex ingredients together with rucinol.

Rucinol (4-n-butyl-resorcinol) is a compound of the following formula (1) and is structurally similar to substances contained in fir trees, and has an excellent inhibitory effect on tyrosinase, an enzyme that causes skin pigmentation due to ultraviolet rays, etc. .

[Formula 1]

The Bidens tripartite is an annual herb of the dicotyledonous plant Campanula Asteraceae. It grows on field banks or near water, and is a plant that often causes damage in fallow rice fields or mountainous rice fields. The hook-like thorn hairs of the above-mentioned lily stick well to other objects, and the young leaves are used for food.

The cosmetic composition may further include euri extract.

The above-mentioned Clematis mandshurica is a deciduous vine that grows in the mountains and fields of various parts of Korea. The leaves are opposite each other and the small leaves are egg-shaped, but the end gradually narrows and the bottom is round or wedge-shaped. The fruits of the above ripen around September and are used for ornamental purposes, the young leaves are edible, and the roots are used for medicinal purposes.

The cosmetic composition may further include buckwheat flower extract.

The buckwheat ( Fagopyrum esculentum ) is an annual plant belonging to the family Gadulaceae and is taxonomically distinct from grains, but is a similar grain with similar characteristics to grains, and contains carbohydrates, proteins, essential amino acids such as lysine, arginine, and methionine, unsaturated fatty acids, and various minerals and vitamins. It contains.

The buckwheat protein is characterized by a high lysine content compared to other grains, with lysine at about 6.1% (w/w) and methionine at about 2.5% (w/w).

The buckwheat flowers bloom in August to September, and because they contain a lot of honey, they are a good source of honey for bees.

The cosmetic composition may further include one or more of Baekhyangaceae extract and Jilryeoberry extract.

The above-mentioned Passiflora edulis is also called Passiflora edulis , and is a perennial evergreen vine plant cultivated in subtropical and tropical regions. It belongs to the genus Passiflora and Passiflora, and about 500 species are distributed around the world. The edible part of the Baekhyang family is a fruit that weighs about 40 to 70 g and has a good flavor and aroma, so it is used raw or in making beverages, processed products, perfume, etc.

The Baekhyanggwa may be made of seeds, pulp, skin, or any combination thereof. Preferably, seeds, pulp, or a combination thereof may be used, but is not limited thereto.

The Tribulus terrestris is also called Baekjiryeo, and is made by drying the mature fruits of Nappasae, an annual or perennial herb belonging to the Tribulus terrestris family.

The above-mentioned plant is known to have certain coercive and diuretic effects, and the fruit of Namgasae, an annual herb, is used for eye diseases.

Meanwhile, the extract can be prepared by immersing and heating crushed natural raw materials in an extraction solvent using an extraction method known in the art.

The extraction solvent is selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane, and 1,3-butylene glycol. It may be one or more solvents selected, preferably ethanol at a concentration (v/v) of 65 to 75%, and two or more different solvents may be mixed or used sequentially, but are not limited thereto.

The extraction ratio of the active ingredients contained in the raw materials may vary depending on the polarity of the solvent, and since ethanol has excellent selectivity in extracting biologically active substances from natural raw materials, the optimal skin improvement effect is realized by the ethanol extraction. It can be.

In particular, water and ethanol have different polarities, so the active ingredients extracted according to each polarity may differ from each other, so the concentration of the ethanol can be appropriately controlled to achieve the optimal skin improvement effect.

The cosmetic composition may further include antioxidant properties.

The term “anti-oxidant” refers to the action of suppressing oxidation. The human body maintains a balance of pro-oxidation and inhibitory substances, but due to various factors, this balance is lost and oxidation is promoted. When tilted in one direction, oxidative stress is induced in the body, which can cause cell damage and pathological diseases.

Reactive oxygen species (ROS), which are the direct cause of the oxidative stress, are chemically unstable and highly reactive, so they can easily react with various biological materials such as DNA, proteins, lipids, and carbohydrates, and can destroy macromolecules in vivo. It attacks cells and tissues, causing irreversible damage or causing mutations, cytotoxicity, and cancer, and can also be a direct cause of aging.

By removing or reducing the reactive oxygen species, an antioxidant effect can be obtained to prevent aging and maintain health.

The cosmetic composition may further include uses for improving wrinkles or preventing photoaging.

The “wrinkle improvement” refers to the phenomenon in which elastic fibers composed of elastin exist together with collagen, and skin elasticity is maintained or improved when the elastin and collagen are sufficiently present. In addition, the term “wrinkle improvement” refers to the phenomenon of suppressing or inhibiting the formation of wrinkles on the skin or relieving already formed wrinkles.

The “anti-photoaging” may be for blocking ultraviolet rays or preventing skin aging induced by UVB.

The “photoaging” is an aging phenomenon caused by ultraviolet rays, an external environmental factor. The ultraviolet rays activate proteolytic enzymes and cause chain breaks and abnormal cross-linking of matrix proteins, resulting in damage to biological components, and repetition of the above mechanism can result in apparent skin aging.

The cosmetic composition can effectively improve skin aging induced by ultraviolet rays, unlike intrinsic aging caused by genetic factors regardless of the external environment.

The cosmetic composition includes softening lotion, nourishing lotion, moisturizing cream, nourishing cream, massage cream, nourishing lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, mask pack, spray, and powder. It may be formulated with one or more selected from the group consisting of, but is not limited thereto.

In addition, the composition for external skin application may be formulated with one or more selected from the group consisting of creams, gels, patches, sprays, ointments, warning agents, lotions, liniment agents, pasta agents, and cataplasma agents, but is limited thereto. no.

The cosmetic composition can be formulated by conventional methods. In formulating the cosmetic composition, for example, the content disclosed in the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995) may be referred to, and the formulation of the skin external preparation In this regard, reference may be made to the content disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA.

Specifically, the cosmetic composition can be prepared in a general emulsified formulation and solubilized formulation. For example, lotion such as softening lotion or nourishing lotion; Emulsions such as facial lotion and body lotion; Creams such as nutritional cream, moisture cream, eye cream, etc.; essence; cosmetic ointment; spray; gel; pack; sunscreen; makeup base; Foundation, such as liquid type, solid type, or spray type; powder; Make-up removers such as cleansing creams, cleansing lotions, and cleansing oils; Alternatively, it may be formulated as a cleansing agent such as cleansing foam, soap, or body wash, but is not limited to this.

In addition to the above essential ingredients, other ingredients may be appropriately mixed in the cosmetic composition and skin external composition in each formulation, depending on the type of formulation or purpose of use, within the range that does not impair the purpose of the present invention.

The cosmetic composition and external skin preparation composition may contain commonly acceptable carriers, such as oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc., may be appropriately mixed. It is not limited to this.

The acceptable carrier may vary depending on the formulation. For example, when formulated into ointments, pastes, creams or gels, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or these are used as carrier ingredients. Mixtures may be used.

When the cosmetic compositions and external skin compositions are formulated as powders or sprays, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or mixtures thereof may be used as carrier ingredients, and in the case of sprays, It may further include propellants such as chlorofluorohydrocarbon, propane, butane or dimethyl ether.

When the cosmetic compositions and compositions for topical skin application are formulated as a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, and benzyl benzoate. , propylene glycol, 1,3-butyl glycol oil can be used, in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan. can be used

When the cosmetic composition and external skin composition are formulated as a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester. The same suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the cosmetic composition and skin external composition are formulated as soap, the carrier ingredients include alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oil, and glycerol. , sugar, etc. may be used.

The cosmetic composition and skin external composition may contain fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, and foaming agents commonly used in the industry depending on the quality or function of the final product. agent), fragrance, surfactant, water, ionic or non-ionic emulsifier, filler, metal ion sequestering agent, chelating agent, preservative, blocking agent, wetting agent, essential oil, dye, pigment, hydrophilic or lipophilic activator, cosmetics It may additionally contain auxiliaries commonly used in the fields of cosmetology or dermatology, such as any other commonly used ingredients.

However, the auxiliaries and their mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

The present invention will be described in further detail through the following examples, but it is obvious that the present invention is not limited by the following examples.

Examples and Comparative Examples

In order to verify the stability and functionality of the rucinol and natural composite components of the present invention, samples were mixed as shown in Tables 1 and 2 below, and examples and comparative examples were set.

For each sample, powdered products were obtained from the market and used.

[Content (parts by weight)] division Example One 2 3 4 5 6 Rucinol 10 10 10 10 10 10 Camantharis Extract 60 30 20 15 15 12 Euari Extract - 30 20 15 15 12 Buckwheat flower extract - - 20 15 15 12 Baekhyang Fruit Extract - - - 15 - 12 Tired of Extract - - - - 15 12

[Content (parts by weight)] division Comparative example One 2 3 4 5 6 7 8 9 10 Rucinol 70 - - - - - 10 10 10 - Camantharis Extract - 70 - - - - - - - 14 Euari Extract - - 70 - - - 60 30 15 14 Buckwheat flower extract - - - 70 - - - 30 15 14 Baekhyang Fruit Extract - - - - 70 - - - 15 14 Tired of Extract - - - - - 70 - - 15 14

Experimental Example 1: Skin safety test

To verify the skin safety of the samples of Examples and Comparative Examples, MTT assay experiments on fibroblasts (HDF) were performed.

The samples of Examples and Comparative Examples were suspended in purified water at concentrations of 50, 100, 200, and 400 μg/mL, respectively, and then cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using the MTT {3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF) were dispensed into each 96-well plate at a concentration of 1×10 ⁴ cells/well and cultured at 37°C and 5% CO ₂ for 48 hours.

The culture medium was removed, and the samples were cultured in medium treated at each concentration for 48 hours, then the medium was removed and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved at 5 mg/mL in PBS, 50 μL was added, and cultured at 37°C and 5% CO ₂ for 48 hours.

100 L of DMSO (Dimethyl sulfoxide) was added per well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was confirmed at any concentration of each sample. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental Example 2: Evaluation of melanin production inhibition activity

To evaluate the effect of the samples of Examples and Comparative Examples on inhibiting melanin production, B16F10, a melanocyte, was cultured.

B16F10, a melanocyte, was dispensed into each well of a 6-well plate to make 8 x 10 ⁴ cells, and then cultured in a cell incubator at 5% CO ₂ and 37°C for 18 hours.

After 18 hours, the medium of B16F10 cells was removed, and the samples of Examples and Comparative Examples were treated with the same concentration and cultured in a cell incubator under 5% CO ₂ and 37°C conditions for 2 days.

After 2 days, cells were recovered from the plate using a scraper and centrifuged for 1 minute to obtain pellet-shaped cells.

1N NaOH was added to the obtained cells and reacted at 95°C for 10 minutes to dissolve melanin.

After 10 minutes, the amount of melanin produced was measured at a wavelength of 475 nm using a microplate reader, and the amount of protein was measured at a wavelength of 595 nm using a BCA assay.

Each experimental group was quantified as “absolute amount of melanin / absolute amount of protein”, and relative quantification was performed between the experimental group and the control group.

α-MSH was used as a melanogenesis inducer, and arbutin was used as a positive control.

division Melanin relative value (%) control group 100 Positive control group 58 Example 1 52 Example 2 49 Example 3 46 Example 4 43 Example 5 44 Example 6 41 Comparative Example 1 64 Comparative Example 2 82 Comparative Example 3 81 Comparative Example 4 75 Comparative Example 5 77 Comparative Example 6 78 Comparative Example 7 64 Comparative Example 8 62 Comparative Example 9 59 Comparative Example 10 71

Referring to Table 3, the sample of Example 1 had excellent melanin inhibitory activity compared to Comparative Examples 1 to 6, and in the case of Examples 2 to 6 to which additional samples were applied simultaneously, the melanin inhibitory activity was further improved.

In particular, the samples of Comparative Examples 7 to 9, which did not contain both rucinol and Camantharis extract, had relatively poor melanin inhibitory activity, so the results suggest a synergistic effect due to the combination of the rucinol and Camantharis extract.

Experimental Example 3: Evaluation of tyrosinase inhibitory activity

In order to evaluate the effect of the samples of Examples and Comparative Examples on inhibiting melanin production, B16F10, a melanocyte, was cultured.

B16F10, a melanocyte, was dispensed into each well of a 6-well plate to make 8 x 10 ⁴ cells, and then cultured in a cell incubator at 5% CO ₂ and 37°C for 18 hours.

After 18 hours, the medium of B16F10 cells was removed, and the samples of Examples and Comparative Examples were treated with the same concentration and cultured in a cell incubator under 5% CO ₂ and 37°C conditions for 2 days.

After 2 days, cells were recovered from the plate using a scraper and centrifuged for 1 minute to obtain pellet-shaped cells.

The obtained cells were lysed using RIPA lysis buffer.

L-DOPA and cell lysate were added to a 96-well plate and reacted at 37°C for 1 hour. Afterwards, the absorbance was measured at 450 nm.

division Tyrosinase activity relative value (%) control group 100 Positive control group 36 Example 1 38 Example 2 35 Example 3 34 Example 4 32 Example 5 33 Example 6 27 Comparative Example 1 49 Comparative Example 2 61 Comparative Example 3 60 Comparative Example 4 56 Comparative Example 5 57 Comparative Example 6 58 Comparative Example 7 47 Comparative Example 8 46 Comparative Example 9 44 Comparative Example 10 51

Referring to Table 4, the sample of Example 1 had excellent skin whitening activity compared to Comparative Examples 1 to 6, and in the case of Examples 2 to 6 where additional samples were applied simultaneously, the skin whitening effect was further improved.

In particular, the samples of Comparative Examples 7 to 9, which did not contain both rucinol and Camantharis extract, had relatively poor skin whitening effects, so the above results suggest synergistic activity due to the combination of rucinol and Gamagsari extract.

Experimental Example 4: Evaluation of skin brightness improvement activity

The efficacy of improving skin brightness was confirmed after applying to the skin using the samples of Examples and Comparative Examples.

Essence-type cosmetics containing the samples of the above examples and comparative examples were prepared, and 80 subjects were divided into 16 groups and applied to each group, and changes in skin brightness were confirmed.

After washing the face before taking a bath, the formulated cosmetic was applied and used daily for 3 weeks. Skin brightness was confirmed using LAB measurement equipment (Konica Minolta CR-300 Chroma Meter).

Next, the difference (L*) in skin color between the measurement time after application of each test substance and the application start time was calculated according to Equation 1 below, and the results are shown in Table 5 below.

The control group was formulated excluding the samples of Examples and Comparative Examples, and arbutin was added to the positive control group.

The whitening effect is determined by comparing the L* of the sample application area and the control area. If the L* value is around 2, whitening of the deposited pigment is evident, and if it is about 1.5 or more, it can be judged to have a whitening effect.

[Equation 1]

ΔL = L value at the time of measurement after application - L value at the start of application

division Brightness change after 3 weeks (ΔL) control group - Positive control group 2.7 Example 1 2.3 Example 2 2.5 Example 3 2.6 Example 4 2.8 Example 5 2.7 Example 6 2.9 Comparative Example 1 1.8 Comparative Example 2 1.6 Comparative Example 3 1.7 Comparative Example 4 1.5 Comparative Example 5 1.4 Comparative Example 6 1.6 Comparative Example 7 1.8 Comparative Example 8 1.7 Comparative Example 9 2.1 Comparative Example 10 2.0

Referring to Table 5, when the sample of Preparation Example was applied directly to the skin, skin brightness was significantly improved after 3 weeks, and a synergistic effect was realized when the sample of Example 1 was applied simultaneously with the samples of Examples 2 to 4. .

Experimental Example 5: Evaluation of free radical scavenging activity

The extracts of Examples and Comparative Examples were suspended in purified water to evaluate free radical scavenging activity.

The DPPH measurement method is a method of measuring the absorbance at 540 nm to determine the extent to which an inhibitor is decolorized by scavenging the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical).

Vitamin C was used as a control, and the same experiment was repeated three times to ensure accuracy of the experiment, and the results are shown in Table 6 below.

[Concentration that eliminates 50% of radical DPPH] division S.C. ₅₀ (μg/mL) Example 1 130.9 Example 2 126.3 Example 3 121.8 Example 4 115.9 Example 5 114.3 Example 6 109.5 Comparative Example 1 162.6 Comparative Example 2 178.2 Comparative Example 3 180.1 Comparative Example 4 178.4 Comparative Example 5 175.8 Comparative Example 6 177.2 Comparative Example 7 158.9 Comparative Example 8 152.5 Comparative Example 9 148.6 Comparative Example 10 168.7 Control group (vitamin C) 128.2

The lower the value of SC ₅₀ , the higher the scavenging activity of DPPH free radicals. Referring to Table 6, the samples of the Examples had significantly better antioxidant effect than the Comparative Examples.

In particular, Example 1, which simultaneously contains rucinol and acacia extract, was significantly superior in free radical scavenging activity compared to Comparative Examples 7 to 9, which lacked some of the extracts, and the results showed a synergistic effect due to the combination of rucinol and acacia extract. suggests.

Experimental Example 6: Test of cell protection effect according to ultraviolet irradiation

The cytoprotective effect of the samples of the examples against UVB was measured.

Fibroblasts at _a ^{concentration of} 1.0

The culture medium was removed and washed twice with PBS to remove any remaining medium. The samples of the examples were replaced with PBS adjusted according to concentration and irradiated with UVB (30mJ/cm ² ) for 5 minutes.

After removing the PBS and removing the remaining sample, the culture was continued for another 24 hours.

MTT was dissolved in PBS at 5 mg/mL, 50L was added, and cultured at 37°C and 5% CO ₂ for 4 hours.

100 μL of dimethyl sulfoxide solution was added to each well to lyse the stained cells, and then the absorbance was measured at 570 nm using a microplate reader (Table 7).

division Relative cells viability(% of control) Example 1 80.5 Example 2 82.5 Example 3 84.0 Example 4 84.6 Example 5 87.9 Example 6 89.6 Comparative Example 1 68.5 Comparative Example 2 51.3 Comparative Example 3 49.6 Comparative Example 4 43.4 Comparative Example 5 52.2 Comparative Example 6 53.6 Comparative Example 7 70.1 Comparative Example 8 69.4 Comparative Example 9 71.8 Comparative Example 10 55.6 Negative control group (no UV irradiation) 100

Referring to Table 7, the sample of the example containing both rucinol and the extract of Violet fern effectively recovered cells damaged by ultraviolet rays compared to Comparative Examples 7 to 9, which lacked some of the components. The results show that the This suggests a synergistic effect.

Experimental Example 7: Evaluation of skin wrinkle improvement activity

To evaluate the effect of improving skin wrinkles, an experiment was conducted as follows.

80 adult women over 30 years of age were divided into 16 groups of 5 people each. After washing their face every morning and evening for 4 weeks, the test subjects applied cosmetics formulated with the samples of Examples and Comparative Examples evenly to the face and allowed to be fully absorbed. .

At this time, the samples of Examples and Comparative Examples were all formulated with the same ingredients.

ANTERA 3D (Miravex, Ireland) was applied to evaluate wrinkle improvement, and instrument measurements were made before using the test substance, after 2 weeks of use, and after 4 weeks of use.

The same tester measured the left eye corner of all test subjects, and for reproducibility of measurements, the same area was measured by overlapping with the image measured before using the test substance.

The captured images were matched using ANTERA pro software, a dedicated software for ANTERA 3D, and the matched measurement area was used for analysis.

The measurement value was analyzed by using the measurement variable, the Indentation index, to analyze the Wrinkles small value, which represents wrinkles on the skin.

The change in Wrinkles small value of the test subject after 4 weeks according to the use of cosmetics formulated with the samples of Examples and Comparative Examples is shown (Table 8). The better the wrinkle improvement effect, the greater the change in Wrinkles small value.

division Wrinkles small average value Example 1 1.36 Example 2 1.41 Example 3 1.43 Example 4 1.50 Example 5 1.52 Example 6 1.58 Comparative Example 1 1.19 Comparative Example 2 1.11 Comparative Example 3 1.09 Comparative Example 4 1.09 Comparative Example 5 1.12 Comparative Example 6 1.10 Comparative Example 7 1.21 Comparative Example 8 1.23 Comparative Example 9 1.22 Comparative Example 10 1.16

Referring to Table 8, the cosmetic composition formulated with the samples of Examples containing both rucinol and Gamagsari extract had a significantly excellent skin wrinkle improvement effect, especially the skin wrinkle improvement effect of Examples 2 to 6 in which additional samples were applied simultaneously. has been further improved.

The description of the present invention described above is for illustrative purposes, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. will be. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. For example, each component described as unitary may be implemented in a distributed manner, and similarly, components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the patent claims described below, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.

Claims (8)

A cosmetic composition for skin whitening containing rucinol and acacia extract as active ingredients. According to paragraph 1,
A cosmetic composition further comprising an extract of Euari. According to paragraph 2,
A cosmetic composition further comprising buckwheat flower extract. According to paragraph 3,
A cosmetic composition further comprising one or more of Baekhyang acetate extract and Achyranthesia extract. According to any one of claims 1 to 4,
A cosmetic composition further comprising an antioxidant use. According to any one of claims 1 to 4,
A cosmetic composition further comprising a use for improving wrinkles or preventing photoaging. According to any one of claims 1 to 4,
A group consisting of softening lotion, nourishing lotion, moisturizing cream, nourishing cream, massage cream, nourishing lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, mask pack, spray and powder. A cosmetic composition formulated with one or more selected substances. An external composition for skin whitening containing rucinol and acacia extract as active ingredients.