KR20230162685A - 아실 CoA 화합물 환원 활성을 갖는 재조합 폴리펩티드 - Google Patents

아실 CoA 화합물 환원 활성을 갖는 재조합 폴리펩티드 Download PDF

Info

Publication number
KR20230162685A
KR20230162685A KR1020237037160A KR20237037160A KR20230162685A KR 20230162685 A KR20230162685 A KR 20230162685A KR 1020237037160 A KR1020237037160 A KR 1020237037160A KR 20237037160 A KR20237037160 A KR 20237037160A KR 20230162685 A KR20230162685 A KR 20230162685A
Authority
KR
South Korea
Prior art keywords
amino acid
substituted
acid residue
phenylalanine
alanine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020237037160A
Other languages
English (en)
Korean (ko)
Inventor
고지 이사카
료 미야타케
사야카 가타야마
Original Assignee
아사히 가세이 가부시키가이샤
아사히 가세이 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 아사히 가세이 가부시키가이샤, 아사히 가세이 가부시키가이샤 filed Critical 아사히 가세이 가부시키가이샤
Publication of KR20230162685A publication Critical patent/KR20230162685A/ko
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1096Transferases (2.) transferring nitrogenous groups (2.6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/001Amines; Imines
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/005Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/24Preparation of oxygen-containing organic compounds containing a carbonyl group
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42Hydroxy-carboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01024Succinate-semialdehyde dehydrogenase (NAD+) (1.2.1.24)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01076Succinate-semialdehyde dehydrogenase (acetylating) (1.2.1.76)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01079Succinate-semialdehyde dehydrogenase (NADP+) (1.2.1.79)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145Clostridium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185Escherichia
    • C12R2001/19Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020237037160A 2021-03-30 2022-03-17 아실 CoA 화합물 환원 활성을 갖는 재조합 폴리펩티드 Pending KR20230162685A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021057313 2021-03-30
JPJP-P-2021-057313 2021-03-30
PCT/JP2022/012463 WO2022209994A1 (ja) 2021-03-30 2022-03-17 アシルCoA化合物還元活性を有する組換えポリペプチド

Publications (1)

Publication Number Publication Date
KR20230162685A true KR20230162685A (ko) 2023-11-28

Family

ID=83456173

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237037160A Pending KR20230162685A (ko) 2021-03-30 2022-03-17 아실 CoA 화합물 환원 활성을 갖는 재조합 폴리펩티드

Country Status (6)

Country Link
US (1) US20240158764A1 (https=)
EP (1) EP4317438A1 (https=)
JP (1) JPWO2022209994A1 (https=)
KR (1) KR20230162685A (https=)
CN (1) CN117083388A (https=)
WO (1) WO2022209994A1 (https=)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011512868A (ja) 2008-03-11 2011-04-28 ディーエスエム アイピー アセッツ ビー.ブイ. アジピン酸(エステルまたはチオエステル)の合成
JP2012188407A (ja) 2011-03-14 2012-10-04 Mitsubishi Chemicals Corp 精製ペンタメチレンジアミンの製造方法及びポリアミド樹脂の製造方法
JP2012525856A (ja) 2009-05-07 2012-10-25 ゲノマチカ, インク. アジペート、ヘキサメチレンジアミン、及び6−アミノカプロン酸の生合成のための微生物及び方法
JP2016501031A (ja) 2012-12-21 2016-01-18 エボニック デグサ ゲーエムベーハーEvonik Degussa GmbH カルボン酸又はジカルボン酸又はそれらのモノエステルからのアミン及びジアミンの製造
JP2016533162A (ja) 2013-09-17 2016-10-27 ズィモケム インコーポレイテッドZymochem Inc. 再生可能資源から化合物を生成するための高収量経路
JP2016538870A (ja) 2013-12-05 2016-12-15 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 脂肪アミンの微生物生産方法
JP2017533734A (ja) 2014-11-14 2017-11-16 インビスタ テクノロジーズ エス.アー.エール.エル. 6−炭素モノマーを産生するための方法および材料
JP2018046843A (ja) 2009-06-04 2018-03-29 ゲノマチカ, インク. 1,4−ブタンジオールの生成のための微生物体及び関連する方法
WO2019159831A1 (ja) 2018-02-15 2019-08-22 国立大学法人神戸大学 組換え宿主細胞及びd-ブタントリオールの新規製造方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY153590A (en) * 2008-12-12 2015-02-26 Celexion Llc Biological synthesis of difunctional alkanes from alpha ketoacids
WO2010126670A2 (en) * 2009-03-27 2010-11-04 Gojo Industries, Inc. Compositions and methods for screening and using compounds antagonizing spore-surface interactions
CA3297132A1 (en) * 2014-12-23 2026-03-02 Genomatica, Inc. Method of producing & processing diamines

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011512868A (ja) 2008-03-11 2011-04-28 ディーエスエム アイピー アセッツ ビー.ブイ. アジピン酸(エステルまたはチオエステル)の合成
JP2012525856A (ja) 2009-05-07 2012-10-25 ゲノマチカ, インク. アジペート、ヘキサメチレンジアミン、及び6−アミノカプロン酸の生合成のための微生物及び方法
JP2018046843A (ja) 2009-06-04 2018-03-29 ゲノマチカ, インク. 1,4−ブタンジオールの生成のための微生物体及び関連する方法
JP2012188407A (ja) 2011-03-14 2012-10-04 Mitsubishi Chemicals Corp 精製ペンタメチレンジアミンの製造方法及びポリアミド樹脂の製造方法
JP2016501031A (ja) 2012-12-21 2016-01-18 エボニック デグサ ゲーエムベーハーEvonik Degussa GmbH カルボン酸又はジカルボン酸又はそれらのモノエステルからのアミン及びジアミンの製造
JP2016533162A (ja) 2013-09-17 2016-10-27 ズィモケム インコーポレイテッドZymochem Inc. 再生可能資源から化合物を生成するための高収量経路
JP2016538870A (ja) 2013-12-05 2016-12-15 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 脂肪アミンの微生物生産方法
JP2017533734A (ja) 2014-11-14 2017-11-16 インビスタ テクノロジーズ エス.アー.エール.エル. 6−炭素モノマーを産生するための方法および材料
WO2019159831A1 (ja) 2018-02-15 2019-08-22 国立大学法人神戸大学 組換え宿主細胞及びd-ブタントリオールの新規製造方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Cheong, et. al. Energy-and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions, Nature biothechnology, 2016
Turk et. al. Metabolic Engineering toward Sustainable Production of Nylon-6, ACS Synth. Biol. 2016, 5, pp.65-73
Yu Zhou, et. al. Biosynthesis of adipic acid by a highly efficient induction-free system in Escherichia Coli, Journal of Biotechnology, Elsevier, 2020, 8, pp.314-315
Yu, et.al. Direct biosynthesis of adipic acid from a synthetic pathway in recombinant Escherichia coli, Biotechinology and Bioengineering, Wiley Periodicals, Inc., 2014, vol.111, pp.2580.

Also Published As

Publication number Publication date
US20240158764A1 (en) 2024-05-16
EP4317438A1 (en) 2024-02-07
CN117083388A (zh) 2023-11-17
JPWO2022209994A1 (https=) 2022-10-06
WO2022209994A1 (ja) 2022-10-06

Similar Documents

Publication Publication Date Title
Maier et al. Molecular characterization of the 56-kDa CYP153 from Acinetobacter sp. EB104
JP2022023169A (ja) 1,4-ブタンジオールおよびその前駆体の生合成のための組成物および方法
Chen et al. Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus
JP2015142577A (ja) 3−ヒドロキシアルカン酸の酵素的脱炭酸によるアルケンの製造法
JP2014506466A (ja) イソ酪酸を製造するための細胞及び方法
Liang et al. Production of 3-hydroxypropionate using a novel malonyl-CoA-mediated biosynthetic pathway in genetically engineered E. coli strain
CN113151201B (zh) 高热稳定性高活性异丁香酚单加氧酶突变体及其应用
KR20150022889A (ko) 생합성 경로, 재조합 세포 및 방법
Fedorchuk et al. Site-directed mutagenesis and stability of the carboxylic acid reductase MAB4714 from Mycobacterium abscessus
US20220348974A1 (en) Biotin synthases for efficient production of biotin
EP3090043A1 (en) 3-hydroxyisovalerate (hiv) synthase variants
US10227617B2 (en) Sequestration of carbon dioxide with hydrogen to useful products
US20240228986A1 (en) Engineered cells, enzymes, and methods for producing cannabinoids
KR20230162685A (ko) 아실 CoA 화합물 환원 활성을 갖는 재조합 폴리펩티드
CN110607335B (zh) 一种烟酰胺腺嘌呤二核苷酸类化合物生物合成方法
CN112368378B (zh) 3-甲基巴豆酸脱羧酶(mdc)变体
US20250019671A1 (en) Recombinant polypeptide having carboxylic acid reducing activity
Zhou et al. Exploring, cloning, and characterization of aryl-alcohol dehydrogenase for β-Phenylethanol production in Paocai
Lee et al. Cloning of srfA operon from Bacillus subtilis C9 and its expression in E. coli
Duan et al. Cloning, expression, characterization and application of atcA, atcB and atcC from Pseudomonas sp. for the production of l-cysteine
Chung et al. Cloning, expression, and characterization of P450 monooxygenase CYP102H1 from Nocardia farcinica
CN116286696B (zh) 一种l-泛解酸内酯脱氢酶及其共表达工程菌在合成d-泛解酸内酯中的应用
JP2020036576A (ja) コエンザイムA(CoA)によるフィードバック阻害を受けないパントテン酸キナーゼ
US7901923B2 (en) Microorganism of Enterobacteriacae genus harboring genes associated with L-carnitine biosynthesis and method of producing L-carnitine using the microorganism
Reshetnikov et al. Alteration of cofactor specificity of the acrylyl-CoA reductase from Escherichia coli

Legal Events

Date Code Title Description
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

D21 Rejection of application intended

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D21-EXM-PE0902 (AS PROVIDED BY THE NATIONAL OFFICE)

PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902