KR20230159871A - 세포 생사 판정 방법, 세포 생사 판정 장치 및 세포 생사 판정 시스템 - Google Patents

세포 생사 판정 방법, 세포 생사 판정 장치 및 세포 생사 판정 시스템 Download PDF

Info

Publication number
KR20230159871A
KR20230159871A KR1020237036082A KR20237036082A KR20230159871A KR 20230159871 A KR20230159871 A KR 20230159871A KR 1020237036082 A KR1020237036082 A KR 1020237036082A KR 20237036082 A KR20237036082 A KR 20237036082A KR 20230159871 A KR20230159871 A KR 20230159871A
Authority
KR
South Korea
Prior art keywords
cell
life
image
death
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020237036082A
Other languages
English (en)
Korean (ko)
Inventor
쇼타 가메이
다카시 모리모토
도모유키 시모다
Original Assignee
후지필름 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 후지필름 가부시키가이샤 filed Critical 후지필름 가부시키가이샤
Publication of KR20230159871A publication Critical patent/KR20230159871A/ko
Pending legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/4833Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • G01N15/1433Signal processing using image recognition
    • G01N15/1463
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • G06T7/0012Biomedical image inspection
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • G06T7/0012Biomedical image inspection
    • G06T7/0014Biomedical image inspection using an image reference approach
    • G06T7/0016Biomedical image inspection using an image reference approach involving temporal comparison
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/60Analysis of geometric attributes
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/60Analysis of geometric attributes
    • G06T7/62Analysis of geometric attributes of area, perimeter, diameter or volume
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/02Identification, exchange or storage of information
    • B01L2300/025Displaying results or values with integrated means
    • B01L2300/027Digital display, e.g. LCD, LED
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0654Lenses; Optical fibres
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012Red blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • G01N2015/144Imaging characterised by its optical setup
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • G01N2015/1452Adjustment of focus; Alignment
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493Particle size
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497Particle shape
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/10Image acquisition modality
    • G06T2207/10016Video; Image sequence
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/10Image acquisition modality
    • G06T2207/10056Microscopic image
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/20Special algorithmic details
    • G06T2207/20081Training; Learning
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/20Special algorithmic details
    • G06T2207/20084Artificial neural networks [ANN]
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/30Subject of image; Context of image processing
    • G06T2207/30004Biomedical image processing
    • G06T2207/30024Cell structures in vitro; Tissue sections in vitro

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Hematology (AREA)
  • Signal Processing (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Geometry (AREA)
  • Optics & Photonics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Quality & Reliability (AREA)
  • Urology & Nephrology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Medical Informatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
KR1020237036082A 2021-04-28 2022-04-13 세포 생사 판정 방법, 세포 생사 판정 장치 및 세포 생사 판정 시스템 Pending KR20230159871A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021076026 2021-04-28
JPJP-P-2021-076026 2021-04-28
PCT/JP2022/017730 WO2022230672A1 (ja) 2021-04-28 2022-04-13 細胞生死判定方法、細胞生死判定装置及び細胞生死判定システム

Publications (1)

Publication Number Publication Date
KR20230159871A true KR20230159871A (ko) 2023-11-22

Family

ID=83848131

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237036082A Pending KR20230159871A (ko) 2021-04-28 2022-04-13 세포 생사 판정 방법, 세포 생사 판정 장치 및 세포 생사 판정 시스템

Country Status (8)

Country Link
US (1) US12399103B2 (https=)
EP (1) EP4317970A4 (https=)
JP (1) JPWO2022230672A1 (https=)
KR (1) KR20230159871A (https=)
CN (1) CN117203525A (https=)
AU (1) AU2022263866B2 (https=)
TW (1) TWI901881B (https=)
WO (1) WO2022230672A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022230673A1 (ja) * 2021-04-28 2022-11-03 富士フイルム株式会社 細胞培養装置及び生細胞濃度調整方法
JP2026017219A (ja) * 2024-07-23 2026-02-04 富士フイルム株式会社 観察装置及び観察方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05184579A (ja) * 1992-01-10 1993-07-27 Fujitsu Ltd 光学的バイオアッセイ方法及び装置
DK200801722A (en) * 2008-12-05 2010-06-06 Unisensor As Optical sectioning of a sample and detection of particles in a sample
US9001200B2 (en) 2010-01-12 2015-04-07 Bio-Rad Laboratories, Inc. Cell characterization using multiple focus planes
JP6284024B2 (ja) * 2014-04-28 2018-02-28 横河電機株式会社 細胞生死判定システム、細胞生死判定方法
GB2556436B (en) * 2015-03-06 2022-01-26 Micromass Ltd Cell population analysis
JP6609967B2 (ja) * 2015-03-31 2019-11-27 株式会社ニコン 判定装置、判定システム、判定プログラム、及び細胞の製造方法
JP6660712B2 (ja) 2015-11-10 2020-03-11 株式会社Screenホールディングス 分類器構成方法および細胞の生死判定方法
JP7309570B2 (ja) 2019-11-05 2023-07-18 株式会社豊田自動織機 内燃機関の制御装置

Also Published As

Publication number Publication date
WO2022230672A1 (ja) 2022-11-03
EP4317970A1 (en) 2024-02-07
JPWO2022230672A1 (https=) 2022-11-03
AU2022263866B2 (en) 2025-08-07
TW202248634A (zh) 2022-12-16
EP4317970A4 (en) 2024-09-11
AU2022263866A1 (en) 2023-11-09
CN117203525A (zh) 2023-12-08
US20240044773A1 (en) 2024-02-08
US12399103B2 (en) 2025-08-26
TWI901881B (zh) 2025-10-21

Similar Documents

Publication Publication Date Title
JP6479676B2 (ja) 流体サンプル中の粒子の分類のためのシステム及び方法
JP5775068B2 (ja) 細胞観察装置および細胞観察方法
JP5745919B2 (ja) 細胞解析方法、細胞解析装置、および細胞解析プログラム
US12399103B2 (en) Cell life-and-death determination method, cell life-and-death determination device, and cell life-and-death determination system
JP2018517209A (ja) 画像分析システムおよび関連する方法
CN112881267B (zh) 细胞解析方法、装置、系统及程序、以及训练的人工智能算法的生成方法、装置及程序
CN102576406A (zh) 小型自动化细胞计数器
JPWO2011132587A1 (ja) 細胞観察装置および細胞観察方法
JPWO2011004568A1 (ja) 受精卵観察の画像処理方法、画像処理プログラム及び画像処理装置、並びに受精卵の製造方法
WO2022137243A1 (en) Optical technique for analyzing insects, shrimp and fish
JP2012039929A (ja) 受精卵観察の画像処理方法、画像処理プログラム及び画像処理装置、並びに受精卵の製造方法
US8940498B2 (en) Biological assays for the characterization of cells using single-cell tracking and uses thereof
Frangos et al. Deep learning image analysis for continuous single-cell imaging of dynamic processes in Plasmodium falciparum-infected erythrocytes
Yang et al. Heterogeneities of zebrafish vasculature development studied by a high throughput light-sheet flow imaging system
HK40104555A (en) Cell life/death determining method, cell life/death determining device, and cell life/death determining system
KR20230158117A (ko) 세포 배양 장치 및 생세포 농도 조정 방법
CN118715549A (zh) 具有基于光设置的细胞分类的生物流体分析仪
Famiglietti Wide-field cone bipolar cells and the blue-ON pathway to color-coded ganglion cells in rabbit retina
DE10361073A1 (de) Verfahren und Vorrichtung zur Aufnahme mikroskopischer Bilder
HK40104556A (en) Cell culture system and method for adjusting viable cell concentration
CN112508951A (zh) 用于确定内质网表型的方法及产品和用于药物筛选的方法
Grimes Image processing and analysis methods in quantitative endothelial cell biology
Réda Zellag et al. CentTracker: a trainable, machine learning-based tool for large-scale analyses of C. elegans germline stem cell mitosis
Margraves From Nearfield Nanoparticle Tracking To Intracellular Vesicle Tracking
Weng Integrating Coherent Anti-Stokes Raman Scattering Imaging and Deep Learning Analytics for High Precision, Real Time, Label Free Cancer Diagnosis

Legal Events

Date Code Title Description
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

P22-X000 Classification modified

St.27 status event code: A-2-2-P10-P22-nap-X000

D21 Rejection of application intended

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D21-EXM-PE0902 (AS PROVIDED BY THE NATIONAL OFFICE)

PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

E13 Pre-grant limitation requested

Free format text: ST27 STATUS EVENT CODE: A-2-3-E10-E13-LIM-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000