KR20230151125A - Composition for improving stress, sleeping induction and improving sleep disturbance comprising rhaponticum uniflorum - Google Patents

Abstract

The present invention relates to a composition for relieving stress, inducing sleep, and improving sleep, comprising extract of Cuckoo root. More specifically, it contains cuckoo extract, which can improve sleep caused by stress by reducing the increase in cortisol in the blood, and promotes the secretion of sleep hormones to induce sleep and improve sleep disorders. , to provide a composition for inducing sleep and improving sleep disorders.

Description

Composition for relieving stress, inducing sleep, and improving sleep disorders containing cuckoo extract {COMPOSITION FOR IMPROVING STRESS, SLEEPING INDUCTION AND IMPROVING SLEEP DISTURBANCE COMPRISING RHAPONTICUM UNIFLORUM}

The present invention relates to a composition for relieving stress, inducing sleep, and improving sleep, comprising extract of Cuckoo root. More specifically, it contains cuckoo extract, which can improve sleep caused by stress by reducing the increase in cortisol in the blood, and promotes the secretion of sleep hormones to induce sleep and improve sleep disorders. , relates to a composition for inducing sleep and improving sleep disorders.

Sleep can be defined from several perspectives, but is mainly defined as a state of immobility in which the ability to perceive and respond to the external environment is reversible, repetitive, and normally suspended. Sleep is achieved by brain activity, but is also deeply linked to physiological changes in other body parts.

Sleep accounts for 1/3 of human lifespan, and is the most fundamental and essential physiological phenomenon, and is a very important factor in maintaining health and mental stability. Chronic lack of sleep and disorders have negative effects on overall physical and mental health, including cardiovascular disease, high blood pressure, memory, learning, metabolic control, weight, immunity, cancer resistance, diabetes, safety accidents, and mood.

Sleep disorder is a very common disease that more than 20% of the population has experienced or is suffering from. Sleep disorder refers to a condition in which a person is unable to get healthy sleep, is unable to remain awake during the day despite getting enough sleep, or has difficulty sleeping or waking up due to a disturbed sleep rhythm.

Sleep disorders can cause a variety of personal and social problems, and can cause learning disabilities, decreased efficiency, traffic accidents, safety accidents, emotional disorders, social adjustment disorders, and dissatisfaction in marital life. Mental problems can also be a major cause, caused by excessive stress, anxiety, tension, and fear. Additionally, if sleep disorders are not properly treated, existing medical, neurological, or psychiatric diseases may worsen or recovery may be delayed, and serious illnesses such as myocardial infarction and stroke may result.

To treat sleep disorders, chemical drugs are used to induce sleep and maintain a sleep state by reversibly suppressing the central nervous system.

Sleeping pills are generally effective in the short-term treatment of insomnia, but because they have various side effects such as drug dependence and cognitive decline, non-pharmacological treatment is recommended in the initial stage, and in severe cases, prescribing sleeping pills is a common treatment for insomnia. Treatment drugs include benzodiazepine-based drugs, non-benzodiazepine-based drugs, benzodiazepine receptor agonists, and sedative antidepressants. However, these drugs have side effects such as the formation of tolerance and dependence when used for a long period of time, as well as anxiety, Symptoms similar to excitement and withdrawal may occur, which not only makes daily life difficult but also becomes a risk factor for other diseases such as cognitive impairment and depression.

Therefore, there is a need to develop a composition that can treat anxiety and insomnia with fewer side effects.

Furthermore, in addition to drug treatment, there is an increasing demand for compositions that improve sleep disorders and enable a good night's sleep without side effects in daily life. In particular, a good night's sleep affects the hormonal balance of the human body and the resulting stress, so it can induce a good night's sleep. Research on compositions that can be used is also continuing.

In particular, stress is emerging as a very important social problem because excessive stress occurs in modern society.

The causes of stress and their physical changes are diverse, but what they have in common is that it increases the function of the pituitary-adrenal system (HPA), which is the sympathetic nervous system, causing hormone secretion, resulting in hyperfunction of the adrenal glands and decreased function of the spleen. It is known that all symptoms caused by stress appear. When stress is added, the weight of the spleen decreases significantly due to a decline in immune function, and the function of the adrenal glands increases due to adrenocorticotropic hormone, which increases the weight, reduces lipids, and promotes the secretion of cortisol. The amount of cortisol tends to increase with age and stress, which also contributes to obesity. Additionally, when stress is added, the adrenal gland secretes hormones that increase cholesterol, blood sugar, and lactate dehydrogenase (LDH) in the blood.

No special drugs have been developed to prevent stress, and chemically synthesized drugs such as tranquilizers, anti-anxiety drugs, and sleeping pills are mainly used to relieve the symptoms of mental and physical diseases caused by stress. However, these drugs are habit-forming. As there are problems with various side effects when taken for a long period of time, various attempts have been made recently to develop functional foods aimed at relieving stress using herbal medicinal ingredients.

Therefore, interest in compositions that relieve stress, improve sleep disorders without side effects, and enable a good night's sleep is increasing.

In particular, the need for alternatives to sleeping pills is increasing for long-term users and those who are restricted from taking them, and the demand for health functional foods to improve sleep is increasing.

KR 10-2020-0125727 A

The purpose of the present invention is to provide a composition for relieving stress, inducing sleep, and improving sleep disorders, which can improve sleep deteriorated by stress by reducing the increase in cortisol in the blood, including a cuckoo extract.

Another object of the present invention is to provide a composition for relieving stress, inducing sleep, and improving sleep disorders, which can improve sleep deteriorated by stress and improve sleep induction and sleep disorders by promoting the secretion of sleep hormones, including a cuckoo extract. It is provided.

Another object of the present invention is to provide a food composition and a pharmaceutical composition for relieving stress, inducing sleep, and improving sleep disorders, which contain natural extracts as active ingredients and do not cause side effects upon long-term administration and administration.

In order to achieve the above object, a composition for relieving stress, inducing sleep, and improving sleep disorders according to an embodiment of the present invention contains Rhaponticum uniflorum extract as an active ingredient.

The composition has the effect of improving sleep deteriorated by stress by reducing the increase in cortisol in the blood.

The composition secretes a hormone selected from the group consisting of tryptophan, serotonin, and melatonin.

The composition further includes Youngia japonica (L.) DC.) extract and Commelina communis (L.) extract.

The composition prevents stress-related diseases selected from the group consisting of depression, insomnia, dyspnea sleep disorder, circadian rhythm sleep disorder, somnambulism, obesity, and high blood pressure.

A food composition according to another embodiment of the present invention is prepared including a composition for relieving stress, inducing sleep, and improving sleep disorders.

A pharmaceutical composition according to another embodiment of the present invention is prepared including the composition for relieving stress, inducing sleep, and improving sleep disorders.

Hereinafter, the present invention will be described in more detail.

In the present invention, “sleep disorder” refers to an inability to get enough sleep or a good night's sleep, which interferes with daily life, such as feeling chronic fatigue, feeling drowsy during the day, or having a headache. This includes cases where you cannot fall asleep easily or when you do fall asleep, you fall asleep lightly and do not get a good night's sleep.

Additionally, it can include insomnia, hypersomnia, circadian rhythm disorder, and abnormal behavior while sleeping.

In the present invention, “sound sleep” refers to a state of deep sleep without tossing and turning for 7 to 8 hours, a sleep so deep that one does not dream or cannot remember how much sleep one has had.

In the present invention, "blood circulation-related diseases" refers to a variety of diseases caused by metabolic problems or caused by blood clots, examples of which are selected from the group consisting of hyperlipidemia, hypertension, arteriosclerosis, myocardial infarction, stroke, and cerebral infarction. It may be one or more diseases.

A composition for relieving stress, inducing sleep, and improving sleep disorders according to an embodiment of the present invention contains Rhaponticum uniflorum extract as an active ingredient.

The extract of Rhaponticum uniflorum is a perennial plant belonging to the Asteraceae family, and is also called Rhaponticum uniflorum or Rhaponticum uniflorum. It grows wild in dry grasslands on the slopes below the mountainside in Korea, and is called ‘cuckoo’ because it is a flower that blooms when it hears the cuckoo’s cry. Cuckoo's flowers are large and beautiful and have high ornamental value, so they are mainly used as plantings in flower beds. The flowers are edible, so they are used for frying or decorating food plates, and are known to have antipyretic and detoxifying effects.

The composition has the effect of improving sleep deteriorated by stress by reducing the increase in cortisol in the blood.

Cortisol is a stress hormone secreted from the adrenal cortex of the kidney. It is secreted in the process of enabling the body to produce maximum energy in response to stimuli such as external stress, and plays a role such as increasing blood pressure and glucose levels. Perform.

Generally, when a threatening situation such as stress occurs, the body produces energy to combat the threat. Among the body's nervous systems, the sympathetic nervous system begins to activate and the adrenal gland produces epinephrine, norepinephrine, Steroid-type hormones are secreted.

Cortisol is a steroid hormone secreted from the adrenal cortex and is also called glucocorticoid because it affects glucose metabolism. The paraventricular nucleus (PVN) of the hypothalamus substantially regulates the secretion of cortisol. The paraventricular nucleus of the hypothalamus secretes CRF (corticotropin-releasing factor), which stimulates the pituitary gland to secrete ACTH (adrenocorticotrophic hormone). This hormone travels through the blood to the adrenal cortex and increases the secretion of cortisol into the blood.

The secreted cortisol releases more blood to each organ of the body to help the body fight against external stimuli such as stress. As a result, pulse and breathing increase. It also tenses the muscles, clarifies the mind and sensitizes the senses to make accurate and quick judgment of the situation. It also concentrates glucose, the body's source of energy, so that it can be delivered directly to the brain. However, the problem is that when excessive stress or chronic stress occurs, the blood concentration of cortisol increases and as a result, appetite increases, leading to fat accumulation. Additionally, blood pressure rises, increasing the risk of high blood pressure, and damage to muscle tissue may also occur. Anxiety and nervousness may continue, and along with weight gain, symptoms such as chronic fatigue, chronic headaches, and insomnia may appear.

Therefore, the composition for relieving stress, inducing sleep, and improving sleep disorders of the present invention has the effect of improving sleep deteriorated by stress by lowering the cortisol content in the blood increased by stress.

The composition secretes a hormone selected from the group consisting of tryptophan, serotonin, and melatonin.

The mechanism for resting through sleep is achieved through the influence of hormones. During the day, serotonin is produced in the body, and at night, melotonin is produced, allowing sleep and rest. If the human body is in a normal state, the surrounding environment becomes dark and melotonin is produced to induce sleep.

Tryptophan is an essential amino acid that must be consumed externally because most animals, including humans, cannot synthesize it on their own. Therefore, the most important role of tryptophan is that it is used as a raw material for various proteins in vivo. In particular, tryptophan is used as a precursor for serotonin, a signaling substance, melatonin, a sleep hormone, vitamin B3, NAD, alkaloids, and auxin, a plant growth hormone. Therefore, it is known that consuming meat or chocolate containing a lot of tryptophan is effective in treating depression and alcoholism.

Serotonin is a hormone that calms the aroused state and maintains composure. In situations that require stress or concentration, stress hormones such as noradrenaline and cortisol are secreted, and serotonin is what controls these and creates a sense of calm.

Additionally, serotonin is the precursor to melatonin, which is naturally secreted in a dark environment and induces sleep. In other words, if serotonin is lacking, it is difficult to produce melatonin, making it difficult to get a good night's sleep.

Therefore, the composition for relieving stress, inducing sleep, and improving sleep disorders of the present invention promotes the secretion of hormones of tryptophan, serotonin, and melatonin, and exhibits the effects of inducing sleep and improving sleep disorders.

The composition prevents stress-related diseases selected from the group consisting of depression, insomnia, dyspnea sleep disorder, circadian rhythm sleep disorder, somnambulism, obesity, and high blood pressure.

The above-mentioned depression is a disease that causes various cognitive and psychosomatic symptoms with low motivation and depression as the main symptoms, leading to a decline in daily functions. It is a serious disease that causes changes in emotions, thoughts, physical condition, and behavior. Environmental factors surrounding nature can have an impact, and these environmental factors are situations that are difficult to deal with in life, such as losing a loved one, financial problems, and strong stress.

The above-mentioned insomnia refers to a sleeping disorder that makes it difficult to fall asleep and a sleep maintenance disorder that causes one to fall asleep but wake up frequently or too early. It occurs in people with irregular sleeping times or habits and experiences environmental changes and psychological stress. As the symptoms worsen.

The above-mentioned somnambulism is a pathological condition in which a person wakes up convulsively during sleep, acts in a fairly orderly manner, and then falls back to sleep, and is also called dream wandering, somnambulism, divorce disease, and sleep disorder. When I come back to my senses, I cannot remember what I said or did last night. Sleepwalking can also be caused by fever, systemic illness, alcohol consumption, sleep deprivation, or mental stress that affects the nervous system. Hormonal changes that occur during adolescence, menstruation, and pregnancy can also cause sleepwalking. Sleepwalking symptoms often appear during times of great physical and mental stress.

The above-mentioned hypertension is a condition in which arterial blood pressure is chronically high, and refers to cases in which systolic blood pressure is more than 140 mmHg or diastolic blood pressure is more than 90 mmHg in adults over 18 years of age. It can also be caused by obesity, etc.

In addition, when you are stressed, the secretion of blood pressure-raising substances (adrenaline) increases in the body, which increases blood pressure. Stress can cause obesity by drinking or overeating, or lack of sleep and increased smoking can cause blood pressure to rise.

In particular, the P. lily extract of the present invention inhibits the formation of blood clots and has the ability to inhibit platelet aggregation, so it is effective in preventing, improving, or treating diseases related to blood circulation.

Blood circulation-related diseases, which include various diseases, include an increase in lipid components such as cholesterol in the blood, which causes poor blood flow, hyperlipidemia that develops when lipid components adhere to the arterial walls, chronic inflammatory reactions, and narrowing of the inner walls of arteries, causing blood vessels to This hardening causes arteriosclerosis, and in the long term, the blood clots generated from this block the coronary arteries and cerebral blood vessels, causing myocardial infarction, stroke, and cerebral infarction, and the drugs currently in use are hepatotoxic when used for a long period of time. It is known to have side effects such as muscle toxicity.

The composition for relieving stress, inducing sleep, and improving sleep disorders further includes Youngia japonica (L.) DC.) extract and Commelina communis extract.

The above-mentioned barley (Youngia japonica (L.) DC.) is a biennial herb belonging to the Asteraceae family, and is also called poribangi, hwangmae, and gourd lily. It is native to Korea and grows anywhere, including in fields, field banks, and roadsides. They are about 15 to 100 centimeters tall and have soft fur all over their bodies. The leaves that grow from the roots spread radially, are pinnate, and are deeply split. It is about 8 to 25 centimeters long and 2 to 6 centimeters wide. There are no leaves or no more than four leaves growing from the stem. Flowers bloom in corymb-shaped panicles at the ends of the stems from May to June. Yellow snowshoes about 8 millimeters in diameter bloom. The fruit is a flat achene and the cap hairs are white.

The chicken's intestine grass (Commelina communis) is an annual herb of the chicken's intestine grass family, and is also called chicken crab and chicken's bottom wash. The stem extends horizontally and is divided into many branches. From June to September, a single blue flower blooms inside the somewhat semi-circular folded bracts, and these flowers are said to wither after a day. Meanwhile, two of the petals are large and blue, while the other one is small and white. There are two complete stamens and they are long, and the other 4 are futile. The fruit opens and the flower diameter is about 2cm. Cultivated species include those with flower diameters of about 4cm and those with large leaves. It mainly grows in fields or roadsides and is distributed throughout Korea. The leaves and stems are edible, and the flowers are used as a dye.

The composition for relieving stress, inducing sleep, and improving sleep disorders of the present invention has an equal or increased effect of reducing the increase in cortisol, a stress hormone, when the composition for relieving stress, inducing sleep, and improving sleep disorders further includes the above-mentioned barley extract and chicken columbine extract in the cuckoo extract. The older you get, the more effective it is in promoting the secretion of tryptophan, serotonin, and melatonin, which can greatly improve the quality of sleep.

Preferably, the composition for relieving stress, improving sleep disorders, and maintaining sound sleep may further include Origanum onites (Pot Marjoram) flower extract and Achillea millefolium extract.

The pot marjoram flower contains Terpinen-4-ol (31.6%), linalyl acetate (7.4%), α-Terpinene (7.1%), cymene (5.3%), and linalool (3.3%), and helps relieve nervous tension and relieve anxiety. It is known to have psychological effects such as stress relief and physiological effects such as blood circulation, pain relief, anti-spasmodics, antiviral, stimulation of vitality, sweating, and nerve calming.

Achillea millefolium or yarrow (common yarrow) is an angiosperm belonging to the Asteraceae family and grows naturally in temperate regions of the northern hemisphere of Asia, Europe, and North America. Although it has been introduced as feed for livestock in areas such as New Zealand and Australia, it is also treated as a weed in those regions, often even in areas where it is native. In New Mexico and southern Colorado, it is called plumajillo, a Spanish word meaning small feather, because of the shape and texture of the leaves. In ancient times, it was called herbal militaris (army herb) because it was used to stop blood flow in wounds.

More preferably, the composition for relieving stress, inducing sleep, and improving sleep disorders of the present invention contains 40 to 60 parts by weight of barley extract, 40 to 60 parts by weight of chicken root extract, and 20 to 30 parts by weight of pot marjoram flower extract, based on 100 parts by weight of cuckoo root. parts and 20 to 30 parts by weight of yarrow.

If it is within the above range, the stress relief, sleep disorder improvement, and sound sleep maintenance effects of the composition for stress relief, sleep disorder improvement, and sound sleep maintenance can be increased, which can greatly improve the quality of sleep. It can be provided as a composition for improving sleep disorders.

In addition, as it is composed of a complex extract, compared to the case where only the cuckoo extract is used as a mixture, the effect of reducing the increase in cortisol, a stress hormone, is equal or increased, and the effect of tryptophan and serotonin in older people is increased. And by promoting the secretion of the hormone melatonin, it is possible to provide a composition for relieving stress, inducing sleep, and improving sleep, which can greatly improve the quality of sleep.

In particular, it has an excellent ability to inhibit platelet aggregation, so it can be used for the prevention or treatment of many blood circulation-related diseases, including thrombosis.

The extract is extracted using water as an extraction solvent. Specifically, to prepare the extract, there are steps of washing the natural product; Drying after washing; Grinding the natural product after drying; Leaching the pulverized material using an extraction solvent; A natural extract can be obtained by including the step of leaching and drying the sample.

The method of preparing the extract may be a conventional extraction method in the art, such as ultrasonic extraction, leaching, and reflux extraction.

The extraction solvent can be used in an amount of 2 to 50 times, more specifically, 2 to 20 times the weight of the sample. For extraction, the sample may be left in the extraction solvent for 1 to 72 hours, more specifically, 24 to 48 hours. After obtaining the extract or fraction, additional methods such as concentration or freeze-drying can be used.

A food composition according to another embodiment of the present invention is prepared including the composition for relieving stress, inducing sleep, and improving sleep disorders.

As a specific example, the food composition can be used to manufacture processed food that can enhance the effects of relieving stress, inducing sleep, and improving sleep disorders. These processed foods include, for example, snacks, beverages, alcoholic beverages, fermented foods, canned foods, processed milk foods, processed meat foods, noodles, etc. Confectionery includes biscuits, pies, cakes, bread, candy, jellies, gum, cereals, etc. Beverages include drinking water, carbonated beverages, functional ionic beverages, functional ionic beverages, juices (e.g., apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), sikhye, etc. Alcoholic beverages include sake, whiskey, soju, beer, liquor, and fruit wine. Fermented foods include soy sauce, soybean paste, and red pepper paste. Canned food includes canned seafood (e.g., canned tuna, mackerel, saury, canned conch, etc.), canned livestock products (canned beef, pork, chicken, turkey, etc.), and canned agricultural products (canned corn, peaches, canned file apple, etc.). Milk processed foods include cheese, butter, yogurt, etc. Processed meat products include pork cutlet, beef cutlet, chicken cutlet, and sausage. Includes sweet and sour pork, nuggets, neobiani, etc. Includes noodles such as raw noodles in sealed packages. In addition, the composition can be used in retort foods, soups, etc.

A pharmaceutical composition according to another embodiment of the present invention is prepared including the composition for relieving stress, inducing sleep, and improving sleep disorders.

The pharmaceutical composition may further include one selected from the group consisting of pharmaceutically acceptable carriers, excipients, and diluents. Specifically, the pharmaceutical composition may be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injection solutions, respectively, according to conventional methods. You can. In the present invention, carriers, excipients, and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, and calcium phosphate. , calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations include the extract and its fractions with at least one excipient such as starch, calcium carbonate, It is prepared by mixing sucrose, lactose, and gelatin. In addition to simple excipients, lubricants such as magnesium styrate and talc are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, and syrups, and may contain various excipients such as wetting agents, sweeteners, fragrances, and preservatives in addition to the commonly used simple diluents such as water and liquid paraffin. there is. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurin, glycerogeratin, etc. can be used.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount, and the term "pharmaceutically effective amount" in the present invention refers to the amount used to treat or prevent a disease with a reasonable benefit/risk ratio applicable to medical treatment or prevention. It refers to a sufficient amount, and the effective dose level is determined by the severity of the disease, the activity of the drug, the patient's age, weight, health, gender, the patient's sensitivity to the drug, the administration time, route of administration, and excretion rate of the composition of the present invention used. Factors including the duration of time, drugs used in combination or concurrently with the compositions of the present invention used, and other factors well known in the medical field. The pharmaceutical composition of the present invention can be administered alone or in combination with a known immunotherapeutic agent. It is important to consider all of the above factors and administer the amount that will achieve the maximum effect with the minimum amount without side effects.

The present invention can provide a composition for relieving stress, inducing sleep, and improving sleep disorders, which can improve sleep deteriorated by stress by reducing the increase in cortisol in the blood, including a cuckoo extract.

The present invention can provide a composition for relieving stress, inducing sleep, and improving sleep disorders, which can improve sleep deteriorated by stress and promote secretion of sleep hormones, including cuckoo extract, to induce sleep and improve sleep disorders. there is.

Another object of the present invention is to provide a food composition and a pharmaceutical composition for relieving stress, inducing sleep, and improving sleep disorders without side effects by long-term administration and administration, including natural extracts as active ingredients.

Figure 1 is an experimental result showing the increase rate of serotonin in a composition according to an embodiment of the present invention.
Figure 2 is an experimental result showing the increase rate of melatonin in a composition according to an embodiment of the present invention.
Figure 3 is an experimental result showing the blood clot formation inhibition ability of a composition according to an embodiment of the present invention.
Figure 4 is an experimental result showing the platelet aggregation inhibition ability of a composition according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of natural extract]

1. Preparation of Cuckoo extract

First, the cuckoo sticks were thoroughly washed under running water and then completely dried naturally. The dried kukkupchae was pulverized in a blender, refluxed and extracted at 80°C for 3 hours, and the filtered liquid was concentrated under reduced pressure at 50°C. Each of the above concentrates was freeze-dried to prepare cuckoo extract (RE).

2. Preparation of other extracts

Barley root extract (YE) and chicken root extract (CE) were prepared in the same manner as the cuckoo extract (RE).

3. Preparation of herbal extract

Port marjoram flower extract (OE) and yarrow extract (AE) were prepared in the same manner as the cuckoo root extract (RE), barley extract (YE), and chicken root extract (CE).

4. Preparation of compositions for relieving stress, inducing sleep, and improving sleep disorders

The prepared above-prepared cuckoo extract (RE), barley extract (YE), chicken extract (CE), port marjoram flower extract (OE), and yarrow extract (AE) were mixed in the content range as shown in [Table 1] below to relieve stress. It was manufactured as a composition for alleviating, inducing sleep, and improving sleep disorders.

SC1 SC2 SC3 SC4 SC5 SC6 SC7 SC8 SC9 SC10 R.E. 100 100 100 100 100 100 100 100 100 100 YE - 30 40 50 60 70 60 60 60 60 C.E. - 30 40 50 60 70 60 60 60 60 O.E. - - - - - - 10 20 30 40 A.E. - - - - - - 10 20 30 40

(Unit: parts by weight)

[Experimental Example 1: Human body safety experiment]

In order to test the human safety of the composition for relieving stress, inducing sleep, and improving sleep disorders of the present invention, the compositions SC1 to SC10 for relieving stress, inducing sleep, and improving sleep disorders prepared in Preparation Example 1 were tested as follows. A test was performed.

toxicity test

In the rat repeated administration toxicity test model, differences in toxicity and side effects were confirmed when administering the compositions SC1 to SC10.

6-week-old male and female rats of the SD strain were divided into 10 rats per group (5 male and female) into administration groups for each composition, and each drug was dissolved in 0.5% MC solution (prepared for use) and then administered 1 time at the same time every morning. Single oral administration was repeated for 13 weeks. A single dose was administered in an amount of 3.75 mg/kg to 5 mg/kg. Afterwards, mortality, general symptoms, weight change, and feed and water intake were observed.

No deaths occurred in any group administered the compositions SC1 to SC10 for inducing stress sleep and improving sleep disorders of the present invention within the experimental period. In light of the above experimental results, it was confirmed that the toxicity of the composition for relieving stress, improving sleep disorders, and inducing deep sleep of the present invention was not a problem.

[Experimental Example 2: Stress Relief Effect Experiment]

Samples and experimental animals

The compositions SC1 to SC10 of the present invention prepared in Preparation Example 1 were used, and 5-week-old male rats (Wistar) and 6-week-old male mice (Hairless) were used as experimental animals. In animal experiments, the control group was raised with AIN-93G as a basic diet, and the experimental group was raised with AIN-93G mixed with each experimental sample.

It consisted of a non-stress positive control group, a stress group, and a stress group administered SC1 to SC10, and each group used 10 mice. The experimental device used a restraint cage as restraint stress, and the experimental diet was a modified diet based on the AIN-93G composition, and the animals were reared for 4 weeks using an ad libitum feeding method. For restraint stress, animals were reared on an experimental diet and restrained using a restraint cage for 3 hours before sacrifice. After anesthesia, they were sacrificed and samples (serum or plasma, etc.) were collected.

The amount of cortisol in the blood was measured as a biomarker to evaluate the stress relieving effect.

The results are shown in Table 2 below.

Non-stress positive control stress SC1 SC2 SC3 SC4 SC5 SC6 SC7 SC8 SC9 SC10 amount of cortisol 5.24±2.21 39.21±4.22 24.52±1.45 21.26±8.10 18.31±5.51 13.21±5.21 10.33±2.13 20.26±8.10 9.12±2.02 8.67±2.15 8.64±4.25 9.84±6.48

(Unit: ㎍/㎗)

According to Table 2, in the stress group administered SC2 to SC6, which is a mixture of cuckoo extract (RE), barley extract (YE), and chicken extract (CE), the amount of cortisol increase due to stress was slightly reduced compared to the non-stress positive control group. confirmed.

In particular, in the case of SC7 to SF10, which further contain pot marjoram flower extract (OE) and yarrow extract (AE), it can be seen that the synergistic effect of mixing each extract produces a synergistic effect beyond a simple combination. .

[Experimental Example 3: Sleep inducing effect experiment]

sleep inducing effect

The same samples and experimental animals as in the above experimental example were used, and the experimental animals were starved without food for 24 hours and then 42 mg/kg of pentobarbital, a sleep inducer, was intraperitoneally administered. Sleep was determined at the time when the turning reflex disappeared, animals that did not sleep within 15 minutes were excluded from the test, and the waking time was determined by the time when the animal returned to turning.

When the compositions SC1 to SC10 of the present invention were administered, the effect on the sleep induction time and sleep maintenance time induced by pentobarbital was observed, and the control group was administered nothing other than pentobarbital (p<0.01).

control group SC1 SC2 SC3 SC4 SC5 SC6 SC7 SC8 SC9 SC10 Sleep induction time (minutes) 4.8±0.3 3.6±0.3 3.1±0.5 3.0±0.4 2.8±0.7 2.4±0.4 2.9±0.3 2.2±0.4 2.1±0.4 2.0±0.1 2.2±0.2 Sleep duration (minutes) 98.1±8.1 126.1±8.3 133.4±8.6 134.8±9.0 138.6±3.6 153.1±9.7 137.9±6.3 158.8±7.4 159.6±2.7 162.1±10.2 160.1±1.3

(Unit: minutes)

As shown in Table 3, in the case of the compositions SC1 to SC10 of the present invention, it was confirmed that the sleep induction time was shortened and the sleep maintenance time was significantly increased compared to the control group.

In particular, in the case of SC7 to SF10, which further contains pot marjoram extract (OE) and yarrow extract (AE) in addition to cuckoo extract (RE), barley extract (YE), and chicken extract (CE), sleep induction time It was confirmed that this was further shortened and the sleep maintenance time further increased.

Sleep hormone secretion effect

The same samples and experimental animals as in the above experimental example were used, and the overall average weight of the experimental animals was 34.545 ± 0.08 g, and there was no significant difference in body weight between each group. To confirm the sleep hormone secretion effect of the compositions SC1 to SC10 of the present invention, water was administered as a control group, and the concentrations of serotonin and melatonin in serum were measured.

The results are shown in Figures 1 and 2 below, and the compositions SC1 to SC10 of the present invention showed an increased rate of serotonin and melatonin concentration compared to the control group.

[Experimental Example 4: Effect on blood circulation-related diseases]

Evaluation of anti-thrombosis efficacy

The same samples and experimental animals as in the above experimental example were used, and after dissolving carrageenan in physiological saline, about 40 ㎕ was injected between the toes of the right hind leg of the experimental animal, and 24 hours, 48 hours, and 72 hours were administered. Afterwards, the length of tail thrombosis was measured with a digital vernier caliper (Mitutoyo, Japan).

The compositions SC1 to SC10 of the present invention were orally administered daily starting 3 days before the carrageenan injection, and on the day of thrombus induction, they were orally administered 1 hour before the carrageenan injection. Thereafter, they were orally administered at 23 hours, 47 hours, or 71 hours after the carrageenan injection. .

The tail thrombus length 72 hours after carrageenan injection was measured and shown in Figure 3 below.

As a result, as can be seen in Figure 3, the average length of tail blood clots observed 72 hours after injecting only carrageenan into experimental animals was 2.80 cm, but when the compositions SC1 to SC10 of the present invention were injected, tail blood clots were formed. It was much shorter than that.

In particular, in the case of SC7 to SF10, which further contains pot marjoram extract (OE) and yarrow extract (AE) in addition to cuckoo extract (RE), barley extract (YE), and chicken extract (CE), the average length is about 1.10 cm ( p<0.01), it was confirmed that it had the best antithrombotic effect.

Platelet aggregation inhibitory effect

After anesthetizing the experimental animal whose tail blood clot length was measured, arterial blood inside the abdominal cavity was slowly collected, the blood was placed in a tube containing 3.2% sodium citrate anticoagulant, and the collected blood was incubated at 1,000 rpm for 10 minutes. It was centrifuged for a while. The separated platelet rich plasma (PRP) was again diluted with platelet poor plasma obtained by centrifugation at 3,000 rpm for 10 minutes, and the platelet count of 4.0×10 ⁸ was used as the standard.

Platelet aggregation inhibitory activity is an optical method of confirming platelet aggregation through PRP, measuring changes in electrical resistance and light source due to platelet aggregation. 245 ㎕ of blood was placed in a cuvette and reacted at 37°C for 10 minutes using a platelet aggregator 700-2 (Chrono-Log. Havertown, PA), and then 2.5 ㎕ each of samples SC1 to SC10 of the compositions of the present invention were added. was added and reacted.

Thereafter, after checking the baseline at regular time intervals, 10 μg/ml of collagen, a substance that promotes platelet aggregation, was added and the mixture was stirred at 1,200 rpm and measured at 37°C for up to 10 minutes.

As a result, as can be seen in Figure 4 below, when comparing the platelet aggregation rate of 100 when reacting only platelets and collagen as a control, it was found that the extract (RE), barley extract (YE), and chicken extract ( A complex extract containing CE), in particular, SC7 to SF10, further comprising port marjoram extract (OE) and yarrow extract (AE) in addition to cuckoo extract (RE), barley extract (YE), and rhododendron extract (CE) It was confirmed that it had excellent platelet aggregation inhibition ability compared to the control group.

[Experimental Example 5: Evaluation of palatability]

A palatability evaluation was conducted on SC1 to SC10. After making SC1 to SC10 into tea, it was provided to 20 adult men and women, and they were asked to evaluate the taste and aroma.

Evaluation scores were requested from 1 to 10 points for each item, and the evaluation results were converted into average scores.

The higher the number, the higher the preference for the index.

SF1 SF2 SF3 SF4 SF5 SF6 SF7 SF8 SF9 SF10 taste 4.5 5.5 6 6 6.5 4.5 7.0 7.0 8.0 7.5 incense 4 4.5 5 6 6.5 5 7.0 7.5 8.0 7.0 Comprehensive preference (average) 4 5 5.5 6 6.5 5 7.0 7.5 8.0 7.5

(Unit: index)

Referring to Table 4 above, it was confirmed that in SC1, preference decreased due to the original taste and aroma of cuckoo chae. However, when using a mixture of other natural extracts of SC2 and SC6, it was confirmed that the preference was excellent.

In particular, SC7 and SC10, which further contain herbal extracts, were confirmed to have relatively excellent taste and aroma, confirming that they can be provided as food compositions with high preference.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. falls within the scope of rights.

Claims (7)

Contains Rhaponticum uniflorum extract as an active ingredient.
Composition for relieving stress, inducing sleep, and improving sleep disorders. According to clause 1,
The composition has the effect of improving sleep deteriorated by stress by reducing the increase in cortisol in the blood.
Composition for relieving stress, inducing sleep, and improving sleep disorders. According to clause 1,
The composition secretes a hormone selected from the group consisting of tryptophan, serotonin, and melatonin.
Composition for relieving stress, inducing sleep, and improving sleep disorders. According to clause 1,
The composition further includes Youngia japonica (L.) DC.) extract and Commelina communis (L.) extract.
Composition for relieving stress, inducing sleep, and improving sleep disorders. According to clause 1,
The composition prevents stress-related diseases selected from the group consisting of depression, insomnia, dyspnea sleep disorder, circadian rhythm sleep disorder, somnambulism, obesity, and high blood pressure.
Composition for relieving stress, inducing sleep, and improving sleep disorders. Comprising a composition for relieving stress, inducing sleep, and improving sleep disorders according to any one of claims 1 to 5.
Food composition. Comprising a composition for relieving stress, inducing sleep, and improving sleep disorders according to any one of claims 1 to 5.
Pharmaceutical composition.