KR20220018649A - Composition for improving stress, sleep disorder and maintaining deep sleep comprising lactic acid bacteria - Google Patents

Abstract

The present invention relates to a composition for relieving stress, alleviating sleep disorders, and maintaining deep sleep, including lactic acid bacteria. More specifically, the present invention provides a composition for relieving stress, alleviating sleep disorders, and maintaining deep sleep which can reduce the increase in cortisol, a stress hormone by including lactic acid bacteria, promote sleep hormone secretion to reduce waking time, and thus can alleviate sleep disorders and maintain deep sleep.

Description

Composition for relieving stress, improving sleep disorders, and maintaining good sleep, including lactic acid bacteria

The present invention relates to a composition for relieving stress, improving sleep disorders, and maintaining good sleep, including lactic acid bacteria. More specifically, it can reduce the increase of cortisol, a stress hormone, including lactic acid bacteria, and promote sleep hormone secretion to shorten the waking time, relieve stress that can maintain sleep disorders and good sleep, improve sleep disorders, and It relates to a composition for maintaining good sleep.

Sleep can be defined in many ways, but it is mainly defined as a state of rest in which the ability to perceive and respond to the external environment is reversible, repetitive, and normally stationary. Although sleep is driven by brain activity, it is closely related to physiological changes in other parts of the body.

Sleep accounts for 1/3 of human lifespan, and it is the most fundamental and essential physiological phenomenon, and it is a very important factor in maintaining health and mental stability. Chronic sleep deprivation and disability negatively affects overall physical and mental health, including cardiovascular disease, high blood pressure, memory, learning, metabolic control, weight, immunity, cancer resistance, diabetes, safety accidents and mood.

Sleep disturbance is a very common disease that has been experienced or suffered by more than 20% of the population. Sleep disturbance can cause a variety of personal and social problems, and cause learning disabilities, decreased efficiency, traffic accidents, safety accidents, emotional disorders, social adjustment disorders, and marital dissatisfaction. In addition, if sleep disorders are not properly treated, medical, neurological, and psychiatric diseases already suffered may worsen or recovery may be delayed, and serious diseases such as myocardial infarction and stroke may result.

Chemical immunity that reversibly suppresses the central nervous system to induce sleep and maintain sleep states is used to treat sleep disorders.

Sleeping pills are generally effective for the short-term treatment of insomnia, but non-pharmacological treatment is recommended at the initial stage due to various side effects such as drug dependence and cognitive decline. As therapeutic drugs, benzodiazepine drugs, non-benzodiazepine drugs, benzodiazepine receptor agonists, and antidepressants with sedative action are used. Symptoms similar to arousal and withdrawal may appear, which is a risk factor for not only difficulty in daily life but also other diseases such as cognitive impairment and depression.

Therefore, there is a demand for the development of a composition capable of treating anxiety and insomnia with fewer side effects.

Furthermore, in addition to drug treatment, there is an increasing demand for a composition that can improve sleep disorders and take a good night's sleep without side effects in daily life. Research on possible compositions is also ongoing.

In particular, since stress is excessively generated in modern society, stress is emerging as a very important social problem.

The causes of stress and changes in the body are diverse, but in common, the function of the pituitary-adrenal system (HPA), which is the sympathetic nervous system, is enhanced to induce hormone secretion, resulting in hyperactivity of the adrenal glands and hypofunction of the spleen. It is known that all symptoms caused by stress appear. When stress is added, the weight of the spleen is significantly reduced due to the decline in immune function, and the adrenal function is enhanced by adrenocorticotropic hormone to increase the weight, decrease lipids, and promote the secretion of cortisol. The amount of cortisol tends to increase with age and stress, which also contributes to obesity. In addition, when stress is added, the adrenal glands secrete hormones to increase cholesterol, blood sugar, lactate dehydrogenase (LDH), and the like in the blood.

No special drugs have been developed to prevent stress, and chemically synthesized drugs such as nerve stabilizers, anti-anxiety drugs, and sleeping pills are mainly used for the purpose of alleviating the symptoms of mental and physical diseases caused by stress. There is a problem that various side effects follow when taken for a long time, and recently, various attempts have been made to develop functional foods for the purpose of relieving stress using herbal ingredients.

Therefore, there is an increasing interest in a composition that relieves stress and improves sleep disorders without side effects and allows you to take a good night's sleep.

KR 10-2013-0090936 A

It is an object of the present invention to provide a composition for relieving stress, improving sleep disorders, and maintaining a good sleep, including lactic acid bacteria.

It is an object of the present invention to provide a composition for relieving stress, improving sleep disorders, and maintaining good sleep, which exhibits a stress relieving effect by reducing an increase in cortisol, a stress hormone, including lactic acid bacteria.

Another object of the present invention is to promote the secretion of sleep hormones, including lactic acid bacteria, to provide a composition for relieving stress, improving sleep disorders and maintaining good sleep, which can maintain sleep disorders and good sleep.

In order to achieve the above object, the composition for relieving stress, improving sleep disorders, and maintaining good sleep according to an embodiment of the present invention includes: lactic acid bacteria; and a natural extract, wherein the natural extract is selected from the group consisting of a thistle (Cirsium japonicum) extract, a dandelion (Taraxacum platycarpum) extract, and mixtures thereof.

The lactic acid bacteria are Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus sakei (Lactobacillus sakei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus vulgaris (Lactobacillus bulgaricus), Lactobacillus casei, Lactobacillus (Lactobacillus casei), Lactobacillus Acidophilus (Lactobacillus acidophilus), Lactobacillus reuteri (Lactobacillus reuteri), Lactobacillus fermentum (Lactobacillus fermentum), Lactobacillus salivarius (Lactobacillus salivarius), Lactobacillus gasseri (Lactobacillus gasseri), Lactobacillus helveticus, Lactobacillus paracasei, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium Animalis (Bifidobacterium animalis), Lactococcus lactis (Lactococcus lactis), Streptococcus thermophilus (Streptococcus thermophilus), Bacillus coagulans (Bacillus coagulans), Pediococcus acidilactici (Pediococcus acidilactici), entero It is selected from the group consisting of interococcus faecium, Enterococcus faecalis, and mixtures thereof.

The composition shows a stress relief effect by reducing the increase in cortisol.

The composition secretes a hormone selected from the group consisting of tryptophan, serotonin, and melatonin, thereby improving sleep disorders and maintaining good sleep.

The composition is to prevent a stress-related disease selected from the group consisting of depression, insomnia, dyspnea sleep disorder, circadian rhythm sleep disorder, and sleepwalking.

The food composition according to another embodiment of the present invention is prepared by including the composition for relieving stress, improving sleep disorders, and maintaining good sleep.

The pharmaceutical composition according to another embodiment of the present invention is prepared by including a composition for relieving stress, improving sleep disorders, and maintaining good sleep.

Hereinafter, the present invention will be described in more detail.

In the present invention, "sleep disorder" means not getting enough sleep or a good night's sleep, so that it interferes with daily life, such as chronic fatigue, drowsiness during the day, or headache. It includes all cases where it is difficult to fall asleep or fall asleep lightly even when falling asleep and unable to get a good night's sleep. In addition, insomnia, hypersomnia, sleep waking time (circadian rhythm, daily rhythm) disorders, and may include all of the abnormal behavior during sleep.

In the present invention, "deep sleep" refers to a state of deep sleep without tossing and turning for 7 to 8 hours, not dreaming, or taking a deep sleep so that you do not remember how much you slept.

A composition for relieving stress, improving sleep disorders and maintaining a good sleep according to an embodiment of the present invention includes: lactic acid bacteria; and a natural extract, wherein the natural extract is selected from the group consisting of a thistle (Cirsium japonicum) extract, a dandelion (Taraxacum platycarpum) extract, and mixtures thereof.

The lactic acid bacteria (LAB) are bacteria that produce a large amount of lactic acid by fermenting sugar. It is a microorganism with important meaning. The major lactic acid bacteria species used around the world are Lactobacillus genus, and functions such as maintenance of normal intestinal flora, immune enhancement, and atopic dermatitis treatment efficacy have been reported. In particular, Lactobacillus plantarum is a lactic acid bacterium belonging to the genus Lactobacillus, which is mainly isolated from kimchi, milk, cheese, and butter, and is one of the most widely distributed lactic acid bacteria in nature. In addition, Lactobacillus plantarum is a bacteria that mainly grows when kimchi is fermented and has a sour taste. It has strong acid resistance and bile resistance, has antibacterial activity against Fusarium and Pseudomonas, and has antioxidant and anti-inflammatory properties. It is a very useful strain because it has an effect.

The composition for relieving stress, improving sleep disorders, and maintaining a good sleep of the present invention is Lactobacillus plantarum, Lactobacillus sakei, Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus vulgaris (Lactobacillus) , Lactobacillus casei (Lactobacillus casei), Lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus reuteri (Lactobacillus reuteri), Lactobacillus fermentum (Lactobacillus fermentum), Lactobacillus salivarius, Lactobacillus salivarius (Lactobacillus salivarius) Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus paracasei, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium animalis, Lactococcus lactis, Streptococcus thermophilus, Bacillus coagulans, Pedio It may include lactic acid bacteria selected from the group consisting of Pediococcus acidilactici, Enterococcus faecium, Enterococcus faecalis, and mixtures thereof.

Preferably, the lactic acid bacteria are Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus sakei (Lactobacillus sakei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus acidophilus (Lactobacillus acidophilus), Bacillus coagulans), Pediococcus acidilactici, Enterococcus faecium, Enterococcus faecalis, and mixtures thereof.

Lactobacillus plantarum (Lactobacillus plantarum) is a bacterium of the genus Lactobacillus commonly found in many fermented foods and anaerobic plant materials, and is mainly classified and used in kimchi. Lactobacillus plantarum (Lactobacillus plantarum) is a gram-positive bacteria, facultative anaerobes that can grow under both aerobic and anaerobic conditions, does not form spores, is not motile, and has a cell form of bacilli.

Lactobacillus plantarum (Lactobacillus plantarum) is a representative lactic acid bacteria classified in kimchi, and in addition, Leuconostoc mesenteroides, Leuconostoc dextranicum, Lactobacillus brevis , Lactobacillus plantarum (Lactobacillus plantarum), and Pediococcus pentosacus (Pediococcus pentosacues) and the like.

When the kimchi lactic acid bacteria or a culture supernatant thereof is used as a food additive, the kimchi-derived lactic acid bacteria may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prophylactic, health or therapeutic treatment).

The Lactobacillus sakei (Lactobacillus sakei) is a bacterial species of the genus Lactobacillus, which functionally belongs to a heterogeneous fermentative Lactobacillus species.

Lactobacillus rhamnosus was originally a bacterium thought to be a subspecies of L. casei, but genetic studies have revealed that it is itself a species. It has high intestinal fixability and is stable to changes in acidity, and is most often used in research on the efficacy of probiotics.

Lactobacillus acidophilus (Lactobacillus acidophilus) is a species of gram-positive bacteria of the genus Lactobacillus. It grows well in acids with a pH of 5 or less, is used to treat ulcerative colitis, and has the ability to kill Staphylococcus aureus. this is excellent

The Bacillus coagulans (Bacillus coagulans) is a bacterial species that forms lactic acid, can alleviate general indigestion, and is mainly used for the treatment of diarrhea caused by rotavirus infection in children.

The Pediococcus acidilactici is a species of Gram-positive cocci and is commonly found in fermented vegetables, fermented dairy products and meat. It is a homogeneous fermenting bacterium that can grow over a wide range of pH, temperature and osmotic pressure.

Enterococcus faecium is a gram-positive, alpha-hemolytic or non-hemolytic bacterium of the genus Enterococcus, which, although symbiotic in the intestinal tract of animals, can also cause neonatal meningitis and endocarditis. It is one of the most common multidrug-resistant bacteria that are resistant to two or more antibiotics.

Enterococcus faecalis, previously classified as part of group D of the Streptococcus family, is a gram-positive, symbiotic bacterium that inhabits the gastrointestinal tract of humans and other mammals. Like other species of the genus Enterococcus, E. faecalis is also found in healthy people, but the naturally high levels of antibiotic resistance found in E. faecalis can cause life-threatening infections through nosocomial (hospital) infections. . E. faecalis is commonly found in teeth treated with root canal (root canal) treatment with a rate of 30-90% of the carrier rate.

Preferably, the lactic acid bacteria contained in the composition for relieving stress, improving sleep disorders and inducing deep sleep is based on 100 parts by weight of Lactobacillus plantarum, 80 parts by weight of Lactobacillus sakeai, 80 parts by weight of Lactobacillus rhamnosus, 80 parts by weight of Lactobacillus acido. It will include 80 parts by weight of fillers, 80 parts by weight of Bacillus coaglance, 80 parts by weight of Pediococcus acidilactisi, 80 parts by weight of Enterococcus faecium and 80 parts by weight of Enterococcus faecium.

The thistle (Cirsium japonicum) is a perennial herb covered with hairs, branching slightly from the upper part and growing upright, and a large one reaches about 1 m. There are large and small thorns on the edge of the leaf. Purple or red flowers bloom one by one at the end of a branch in midsummer. Each flower contains hundreds of small, barrel-shaped flowers. The fruit has hairs, and when it matures, it is blown by the wind to propagate. The young leaves are used as herbs, and the whole is dried and used for medicinal purposes. Similar plants include large thistle, fin thistle, and Korean thistle.

The dandelion (Taraxacum platycarpum) is a dicotyledonous perennial plant of the order Asteraceae of the order Asteraceae, a plant belonging to the dicotyledonous family of the angiosperm phylum, and is grown in a sunny place in a field. There is no stem, and the leaves are clustered at the root and spread laterally. The leaves are in the shape of an upside down lancet, 6-15cm long and 1.2-5cm wide, in the shape of a pinnate deeply indented, with serrated edges and some hairs.

The composition for relieving stress, improving sleep disorders, and maintaining good sleep exhibits a stress relieving effect by reducing an increase in cortisol.

Cortisol is a stress hormone secreted by the adrenal cortex of the kidneys. It is secreted in the process of allowing the body to generate maximum energy in response to stimuli such as external stress, and plays the same role as raising blood pressure and glucose levels. do.

In general, when a threatening situation such as stress comes, the body produces energy to counter the threat, and the sympathetic nervous system of the body's nervous system starts to work, and the adrenal gland releases epinephrine, norepinephrine, and Steroid hormones are secreted.

Cortisol is a steroid hormone secreted by the adrenal cortex and is also called a glucocorticoid because it affects glucose metabolism. The paraventricular nucleus (PVN) of the hypothalamus actually controls the secretion of cortisol. The subthalamic nucleus of the hypothalamus secretes corticotropin-releasing factor (CRF), which stimulates the pituitary gland to secrete adrenocorticotrophic hormone (ACTH). This hormone travels through the blood to the adrenal cortex and increases the secretion of cortisol into the blood.

The released cortisol causes more blood to be released into each organ in the body to help the body fight against external stimuli such as stress. As a result, pulse and respiration increase. It also sharpens the mind and sharpens the senses in order to tense the muscles and make accurate and quick situational judgments. It also concentrates glucose, the energy source of our body, so that it can be delivered directly to the brain. However, the problem is that when you are stressed or chronically stressed, the blood level of cortisol increases and as a result, appetite increases, resulting in the accumulation of fat. In addition, blood pressure rises, which increases the risk of hypertension, which can also cause muscle tissue damage. Anxiety and agitation may lead, and symptoms such as chronic fatigue, chronic headache, and insomnia may appear along with weight gain.

Therefore, in the case of the stress relief effect of the composition for relieving stress, improving sleep disorders and maintaining good sleep of the present invention, a natural product without toxicity and side effects to the human body is used to reduce the increase in cortisol, a stress hormone.

The composition secretes a hormone selected from the group consisting of tryptophan, serotonin, and melatonin, thereby improving sleep disorders and maintaining good sleep.

The mechanism of resting through sleep is made by the influence of hormones. During the day, serotonin is produced in the body and melotonin is produced at night, which causes sleep and rest. If the human body is in a normal state, the surrounding environment darkens and melotonin is produced to induce sleep.

The tryptophan (tryptophan) is an essential amino acid that must be ingested from the outside because most animals, including humans, cannot synthesize by themselves. Therefore, the most important role of tryptophan is that it is used as a raw material for various proteins in vivo. In particular, tryptophan is used as a precursor to serotonin, a signaling substance, melatonin, a sleep hormone, vitamin B3, and NAD, alkaloids, and auxin, a plant growth hormone. Therefore, eating meat or chocolate containing a lot of tryptophan is effective in treating depression and is known to be effective in alcoholism.

The serotonin (Serotonin) is a hormone that calms an aroused state and maintains composure. Stress hormones such as noradrenaline and cortisol are secreted in situations that require stress or concentration, and it is serotonin that controls them and makes them calm.

In addition, serotonin is a precursor to melatonin that is naturally secreted in a dark environment to induce sleep. In other words, when serotonin is insufficient, it is difficult to get a good night's sleep because it is difficult to produce melatonin.

Accordingly, the composition for relieving stress, improving sleep disorders and maintaining good sleep of the present invention promotes the secretion of hormones of tryptophan, serotonin, and melatonin, and exhibits the effect of improving sleep disorders and maintaining good sleep.

The composition is to prevent a stress-related disease selected from the group consisting of depression, insomnia, dyspnea sleep disorder, circadian rhythm sleep disorder, and sleepwalking.

The depression is a disease that causes a decrease in daily functions by causing various cognitive and psychosomatic symptoms with a decrease in motivation and depression as the main symptoms, and is a serious disease that causes changes in emotions, thoughts, physical conditions, and behaviors. Environmental factors surrounding nature can have an impact, and these environmental factors are difficult situations in life, such as losing a loved one, economic problems, and strong stress.

The above insomnia refers to a sleep disorder in which it is difficult to fall asleep and a sleep maintenance disorder in which a person falls asleep but frequently wakes up during sleep or wakes up too early. and the symptoms worsen.

The sleepwalking is a pathological symptom in which a person wakes up paroxysmal during sleep and falls back to sleep after performing a fairly orderly behavior, and is also called mongjung wandering, mongjung paradox, divorce disease, sleepgyeongbyeong, and the like. When I come back to my senses, I can't remember the last night's words and actions. Sleepwalking can also be caused by heat that affects the nervous system, systemic illness, alcohol consumption, sleep deprivation, or mental stress. Hormonal changes that occur during adolescence, menstruation, and pregnancy can also cause sleepwalking. Sleepwalking symptoms often appear during periods of high physical and mental stress.

Preferably, the composition for relieving stress, improving sleep disorders, and maintaining good sleep includes 40 to 60 parts by weight of a milk thistle extract and 40 to 60 parts by weight of a dandelion extract based on 100 parts by weight of lactic acid bacteria.

According to the above range, equal or increase the effect that can reduce the increase in cortisol, a stress hormone, tryptophan (tryptophan), serotonin (Serotonin) and melatonin (melatonin) to promote hormone secretion of older age, It is effective in greatly improving the quality of sleep.

More preferably, the composition for relieving stress, improving sleep disorders, and maintaining a deep sleep may further include a New Green extract, a wheatgrass extract and a honeysuckle extract.

The New green (New green) is a kind of broccoli of the cruciferous family, also called Toscano (Toscano). Broccoli mainly eats flower buds and flower stalks, but New Green is leaf broccoli that eats leaves. The leaves are blackish green, and the front side is convex. It is relatively easy to cultivate as it has strong resistance to cold, moisture, and disease. When the leaves grow to the size of a palm, they are harvested by plucking them one by one. It should be noted that after sowing in early spring and after a certain period of time below 7℃, the overtaking phenomenon may occur, which may decrease the value of the product.

The wheat sprout refers to young shoots of wheat, and is a vegetable grown by sprouting wheat seeds. It grows by laying soil on the bed, planting whole wheat soaked in water, and then watering it just like when growing bean sprouts. Before germination, place it in a shaded place, and when germination occurs, move it to a sunny and well-ventilated place. After a week or so, when the buds have grown to about 15 cm, cut them and use them. When harvesting, the root is cut and the stem is cut, and after cutting, the shoot grows again, so it can be eaten several times. Wheat sprouts are often consumed as juice by extracting the green juice with a juicer.

The Lonicera japonica stem is reddish-brown, and is wound up to the right, and young branches have many yellowish-brown hairs and are hollow. The leaves are opposite, long oval, acute, round-bottomed. There are hairs on the petiole, and the hairs on the leaves disappear or remain only on the back side. The edges are flat, but the leaves on the root side may have dents. Two lip-shaped white flowers bloom from the axil of the leaf, and the calyx has no hair, and the end of the corolla is split into 5 parts, and one of them is deeply split and dried back. There are 5 stamens and 1 pistil. The flowering period is from June to July. Round fruits ripen to black in September-October. Because the color of the flower changes from white to yellow, it is also called '金銀花'. It is a semi-evergreen broad-leaved vine that grows wild in mountains and fields across the country. It grows well on the edge of mountains and forests, but it grows well wherever there is sunlight and has strong pollution resistance. In the central region, the leaves fall, but in the southern region, the leaves do not fall and they spend the winter as it is.

More preferably, the composition for relieving stress, improving sleep disorders and maintaining a good sleep of the present invention is based on 100 parts by weight of lactic acid bacteria, 40 to 60 parts by weight of thistle extract, 40 to 60 parts by weight of dandelion extract, 20 to 30 parts by weight of New Green extract, wheat sprout 20 to 30 parts by weight of the extract and 20 to 30 parts by weight of the extract of Hemp extract.

According to the above range, it is possible to increase the stress relief, sleep disorder improvement and sound sleep maintenance effect of the composition for relieving stress, improving sleep disorder and maintaining good sleep, thereby relieving stress that can greatly improve the quality of sleep, improving sleep disorder And it can be provided as a composition for maintaining a good sleep.

In addition, according to the composition of the complex extract, compared to the case of using only lactic acid bacteria, thistle extract, and dandelion extract as a mixture, the effect that can reduce the increase in cortisol, a stress hormone, is equal or increased, and tryptophan at an older age , serotonin (Serotonin) and melatonin (melatonin) by promoting the hormone secretion, it is possible to provide a composition for relieving stress, improving sleep disorders, and maintaining a good sleep that can greatly improve the quality of sleep.

The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.

Specifically, washing the natural product to prepare the extract; drying after washing; pulverizing the natural product after drying; leaching the pulverized product using an organic solvent; drying the sample after leaching; leaching with water; And including the step of leaching, it is possible to obtain a natural extract.

The natural extract extracted using the organic solvent may further comprise the step of performing fractionation using an organic solvent.

The method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances have been removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract extracted by sequentially applying the solvents to the sample.

The reflux extraction method is water, based on 100 mL of an alcohol having 1 to 6 carbon atoms, 10 to 30 g of a pulverized product of a natural product, a reflux time of 1 to 3 hours, and 50 to 100% alcohol or water having 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol having 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of a pulverized product of a natural product, a reflux time of 1 to 2 hours, and 70 to 90% alcohol or water having 1 to 4 carbon atoms. .

The leaching method is carried out at 15 to 30° C. for 24 to 72 hours, and water or 50 to 100% alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, the process is carried out at 20 to 25° C. for 30 to 54 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

In the ultrasonic extraction method, the reaction is performed at 30 to 50° C. for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% alcohol having 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50° C. for 1 to 2.5 hours, and the extraction solvent is water or 70 to 80% alcohol having 1 to 6 carbon atoms.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for extraction in the extraction solvent, and more specifically, for 24 to 48 hours.

After extraction, the extract may be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used for fractionation is any one or more selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride and mixtures thereof, preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or freeze-drying may be additionally used.

The food composition according to another embodiment of the present invention is prepared by including the composition for relieving stress, improving sleep disorders, and maintaining good sleep.

As a specific example, by using the food composition, it is possible to prepare a processed food capable of relieving stress, improving sleep disorders, and enhancing the effect of maintaining a good sleep. Such processed foods include, for example, sweets, beverages, alcoholic beverages, fermented foods, canned foods, milk products, processed meat foods, noodles, and the like. Confectionery includes biscuits, pies, cakes, breads, candies, jellies, gums, cereals, and the like. The beverage includes drinking water, carbonated beverage, functional ionized beverage, functional ionized beverage, juice (eg, apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), sikhye, and the like. Alcoholic beverages include sake, whiskey, shochu, beer, Western liquor, fruit wine, and the like. Fermented foods include soy sauce, soybean paste, red pepper paste, and the like. Canned foods include canned seafood (eg, canned tuna, mackerel, saury, conch, etc.), canned livestock products (canned beef, pork, chicken, turkey, etc.), and canned agricultural products (canned corn, peaches, file apples, etc.). Milk products include cheese, butter, yogurt, and the like. Processed meat products include pork cutlet, beef cutlet, chicken cutlet, and sausage. Includes sweet and sour pork, nuggets, and breadcrumbs. Noodles such as sealed packaged raw noodles are included. In addition to this, the composition may be used in retort foods, soups, and the like.

The pharmaceutical composition according to another embodiment of the present invention is prepared by including a composition for relieving stress, improving sleep disorders, and maintaining good sleep.

The pharmaceutical composition may further include one selected from the group consisting of pharmaceutically acceptable carriers, excipients and diluents. Specifically, the pharmaceutical composition is each formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories and sterile injection solutions according to conventional methods to be used. can In the present invention, carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate , calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the extract and its fractions, for example, starch, calcium carbonate, It is prepared by mixing sucrose or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to simple diluents such as water and liquid paraffin, which are commonly used for suspensions, solutions, emulsions, and syrups. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

The pharmaceutical composition of the present invention may be administered in a pharmaceutically effective amount, the term "pharmaceutically effective amount" of the present invention means to treat or prevent a disease at a reasonable benefit/risk ratio applicable to medical treatment or prevention. It means a sufficient amount, and the effective dose level is the severity of the disease, the drug activity, the patient's age, weight, health, sex, the patient's sensitivity to the drug, the time of administration of the composition of the present invention used, the route of administration and the rate of excretion. The duration may be determined according to factors including drugs used in combination or concomitant with the composition of the present invention used and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered alone or in combination with a known immunotherapeutic agent. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects.

The present invention may provide a composition for relieving stress, improving sleep disorders, and maintaining a good sleep, including lactic acid bacteria.

The present invention may provide a composition for relieving stress, improving sleep disorders, and maintaining good sleep, which exhibits a stress relieving effect by reducing an increase in cortisol, a stress hormone, including lactic acid bacteria.

The present invention can provide a composition for relieving stress, improving sleep disorders, and maintaining a good night's sleep, which can improve sleep disorders and significantly improve the quality of sleep by shortening the waking time to get a good night's sleep.

1 is an experimental result showing the increase rate of serotonin in a composition for stress relief, sleep disorder improvement, and deep sleep induction according to an embodiment of the present invention.
2 is an experimental result showing the increase rate of melatonin in the composition for stress relief, sleep disorder improvement, and deep sleep induction according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of natural extract]

1. Preparation of thistle extract

First, the thistle was thoroughly washed under running water and then completely air-dried. The dried thistle was pulverized with a blender and leached for 48 hours at room temperature using 70% ethanol, respectively, and then the sample was filtered to prepare a thistle extract (CE).

2. Preparation of other extracts

Dandelion extract (TE), New green extract (NE), wheat sprout extract (WE), and Honeysuckle extract (LE) were prepared in the same manner as the thistle extract (CE).

3. Production of lactic acid bacteria

Lactobacillus plantarum, Lactobacillus sakeai, Lactobacillus rhamnosus, Lactobacillus acidophilus, Bacillus coaglance, Pediococcus aciddilactisi, Lactobacillus acidophilus, Lactobacillus acidophilus, Enterococcus faecium and Enterococcus fagalis were given, and with respect to 100 parts by weight of Lactobacillus plantarum, 80 parts by weight of Lactobacillus sakeai, 80 parts by weight of Lactobacillus rhamnosus, 80 parts by weight of Lactobacillus acidophilus Part, Bacillus coagulans 80 parts by weight, Pediococcus acidilactisi 80 parts by weight, Enterococcus faecium 80 parts by weight, and Enterococcus fagalis 80 parts by weight were mixed to prepare lactic acid bacteria (LB).

4. Preparation of a composition for relieving stress, improving sleep disorders and inducing deep sleep

Mix the prepared mixed lactic acid bacteria (LE), milk thistle extract (CE), dandelion extract (TE), new green extract (NE), wheat sprout extract (WE) and honeysuckle extract (LE) in the content range as shown in [Table 1] below Thus, it was prepared as a composition for relieving stress, improving sleep disorders, and inducing deep sleep.

SC1 SC2 SC3 SC4 SC5 SC6 SC7 SC8 SC9 SC10 LE 100 100 100 100 100 100 100 100 100 100 CE - 30 40 50 60 70 60 60 60 60 TE - 30 40 50 60 70 60 60 60 60 NE - - - - - - 10 20 30 40 WE - - - - - - 10 20 30 40 LE - - - - - - 10 20 30 40

(Unit: parts by weight)

[Experimental Example 1: Human stability test]

In order to test the safety of the human body for the composition for stress relief, sleep disorder improvement, and deep sleep induction of the present invention, the following safety tests were performed on the compositions SC1 to SC10 for stress relief, sleep disorder improvement and deep sleep induction prepared in Preparation Example 1 was performed.

Toxicity test

In the rat repeated administration toxicity test model, differences in toxicity and side effects were confirmed when the compositions SC1 to SC10 were administered.

Six-week-old male and female SD rats were divided into 10 rats per group (5 males and females each) and classified into groups administered with each composition, and each drug was dissolved in 0.5% MC solution (prepared for Yongsi), and then 1 Dosing twice orally was repeated for 13 weeks. A single dose was administered in an amount of 3.75 mg/kg to 5 mg/kg. Thereafter, mortality, general symptoms, weight change, and feed and water intake were observed.

No deaths occurred in all groups administered with the compositions SC1 to SC10 for stress relief and fatigue recovery of the present invention within the experimental period. In light of the above experimental results, it was confirmed that the toxicity of the composition for stress relief, sleep disorder improvement, and deep sleep induction of the present invention is not a problem.

[Experimental Example 2: Stress Relief Effect Experiment]

Samples and laboratory animals

Compositions SC1 to SC10 of the present invention prepared in Preparation Example 1 were used, and as experimental animals, a 5-week-old male Rat (Wistar) and a 6-week-old mouse (Hairless) male were used. In the animal experiment, the control group was bred with AIN-93G as a basic diet, and in the case of the experimental group, each test sample was mixed with AIN-93G.

It consisted of a non-stress positive control group, a stress group, and a stress group administered with SC1 to SC10, and each group used 10 mice. As the experimental equipment, cages were used as restraint stress, and the experimental diet was a modified diet based on the composition of AIN-93G, and was bred for 4 weeks in a free feeding method. For restraint stress, after breeding with an experimental diet, they were restrained using a restraint cage for 3 hours before sacrifice, and samples (serum or plasma, etc.) were collected after sacrificing after anesthesia.

As a biomarker for evaluating the effect of stress relief, the amount of cortisol in the blood was measured.

The results are shown in Table 2 below.

amount of cortisol non-stress positive control 5.24±2.21 stress group 39.21±4.22 SC1 24.52±1.45 SC2 21.26±8.10 SC3 18.31±5.51 SC4 13.21±5.21 SC5 10.33±2.13 SC6 20.26±8.10 SC7 9.12±2.03 SC8 8.67±2.28 SC9 8.66±4.26 SC10 9.84±6.48

(Unit: ㎍/㎗)

According to Table 2, it was confirmed that the stress group administered with a mixture of lactic acid bacteria, thistle extract, and dandelion extract SC2 to SC6 had a slightly reduced amount of cortisol increase due to stress compared to the non-stress positive control group.

In particular, it can be seen that, in the case of SC7 to SF10, which further includes a New Green extract, a wheatgrass extract and a honeysuckle extract, a synergistic effect more than a simple combination appears due to the synergistic action by mixing each extract.

[Experimental Example 3: Sleep disorder improvement and sleep induction effect experiment]

Sleep inducing effect

The same samples and experimental animals as in the above experimental example were used, and after the animals were fasted without feeding for 24 hours, pentobarbital 42 mg/kg, a sleep inducer, was administered intraperitoneally. Sleep was defined as the time at which the clove reflex disappeared, animals that did not fall asleep within 15 minutes were excluded from the test, and the waking time was defined as the time the animal returned to the clove.

When the compositions SC1 to SC10 of the present invention were administered, the effects on pentobarbital-induced sleep induction time and sleep maintenance time were observed, and the control group did not administer anything except pentobarbital (p<0.01).

Sleep induction time (minutes) Sleep duration (min) control 4.8±0.3 98.1±8.1 SC1 3.6±0.3 126.1±8.3 SC2 3.1±0.5 133.4±8.6 SC3 3.0±0.4 134.8±9.0 SC4 2.8±0.5 137.6±3.6 SC5 2.4±0.3 153.1±11.7 SC6 2.9±0.2 136.9±6.3 SC7 2.2±0.4 158.8±7.4 SC8 2.1±0.4 159.6±2.7 SC9 2.0±0.5 162.1±10.2 SC10 2.2±0.2 160.1±1.3

As shown in Table 3, in the case of the compositions SC1 to SC10 of the present invention, it was confirmed that the sleep induction time was shortened compared to the control group, and the sleep maintenance time was significantly increased.

In particular, it was confirmed that, in the case of SC7 to SF10, which further includes a New Green extract, a wheatgrass extract and a honeysuckle extract, the sleep induction time is further shortened and the sleep maintenance time is further increased.

sleep hormone secretion

The same samples and experimental animals were used as in the above experimental example, and the average body weight of the experimental animals was 34.545 ± 0.08 g, and there was no significant difference in body weight between each group. In order to confirm the sleeping hormone secretion effect of the compositions SC1 to SC10 of the present invention, water was administered as a control, and the concentrations of serotonin and melatonin in the serum were measured.

The results are shown in FIGS. 1 and 2 below, and the compositions SC1 to SC10 of the present invention confirmed the increase rates of serotonin and melatonin concentrations compared to the control group.

[Experimental Example 4: Evaluation of palatability]

Preference evaluation was performed for the SC1 to SC10. After preparing SC1 to SC10 as tea, and providing it to 20 adult men and women, evaluation of taste and aroma was requested.

The evaluation score was requested to be evaluated on a scale of 1 to 10 points for each item, and the evaluation result was converted into an average score.

In the index, the higher the number, the higher the degree of preference.

SF1 SF2 SF3 SF4 SF5 SF6 SF7 SF8 SF9 SF10 taste 4.5 5.5 6 6 6.5 4.5 7.0 7.0 8.0 7.5 incense 4 4.5 5 6 6.5 5 7.0 7.5 8.0 7.0 Overall preference (average) 4 5 5.5 6 6.5 5 7.0 7.5 8.0 7.5

(Unit: Index)

Referring to Table 4, it was confirmed that in SC1, the taste and aroma of the mixed lactic acid bacteria decreased due to the taste. However, when using a mixture of other natural extracts of SC2 and SC6, it was confirmed that the palatability is excellent.

In particular, in the case of SC7 and SC10, it was confirmed that they exhibit relatively excellent taste and aroma, and it was confirmed that they could be provided as a food composition with high preference.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

Lactobacillus; and
Contains natural extracts,
The natural extract is selected from the group consisting of thistle (Cirsium japonicum) extract, dandelion (Taraxacum platycarpum) extract, and mixtures thereof.
A composition for relieving stress, improving sleep disorders and maintaining good sleep. The method of claim 1,
The lactic acid bacteria are Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus sakei (Lactobacillus sakei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactobacillus vulgaris (Lactobacillus caseilus), Lactobacillus (Lactobacillus casei), Lactobacillus Acidophilus (Lactobacillus acidophilus), Lactobacillus reuteri (Lactobacillus reuteri), Lactobacillus fermentum (Lactobacillus fermentum), Lactobacillus salivarius (Lactobacillus salivarius), Lactobacillus gasseri (Lactobacillus gasseri), Lactobacillus helveticus, Lactobacillus paracasei, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium Animalis (Bifidobacterium animalis), Lactococcus lactis (Lactococcus lactis), Streptococcus thermophilus (Streptococcus thermophilus), Bacillus coagulans (Bacillus coagulans), Pediococcus acidilactici (Pediococcus acidilactici), Cus faecium (nterococcus faecium), Enterococcus faecalis (Enterococcus faecalis) and selected from the group consisting of mixtures thereof
A composition for relieving stress, improving sleep disorders and maintaining good sleep. The method of claim 1,
The composition shows a stress relieving effect by reducing an increase in cortisol
A composition for relieving stress, improving sleep disorders and maintaining good sleep. The method of claim 1,
The composition secretes a hormone selected from the group consisting of tryptophan, serotonin, and melatonin, thereby improving sleep disorders and maintaining good sleep.
A composition for relieving stress, improving sleep disorders and maintaining good sleep. The method of claim 1,
The composition is for preventing stress-related diseases selected from the group consisting of depression, insomnia, dyspnea sleep disorder, circadian rhythm sleep disorder, and sleepwalking.
A composition for relieving stress, improving sleep disorders and maintaining good sleep. According to any one of claims 1 to 5, comprising a composition for relieving stress, improving sleep disorders, and maintaining a good sleep
food composition. According to any one of claims 1 to 5, comprising a composition for relieving stress, improving sleep disorders, and maintaining a deep sleep according to any one of claims 1 to 5
pharmaceutical composition.

Images