KR20230121076A - Dna 샘플의 시퀀스에 있어서의 어댑터 결합 효율을평가하는 방법 - Google Patents
Dna 샘플의 시퀀스에 있어서의 어댑터 결합 효율을평가하는 방법 Download PDFInfo
- Publication number
- KR20230121076A KR20230121076A KR1020237021963A KR20237021963A KR20230121076A KR 20230121076 A KR20230121076 A KR 20230121076A KR 1020237021963 A KR1020237021963 A KR 1020237021963A KR 20237021963 A KR20237021963 A KR 20237021963A KR 20230121076 A KR20230121076 A KR 20230121076A
- Authority
- KR
- South Korea
- Prior art keywords
- adapter
- dna
- double
- binding
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000009739 binding Methods 0.000 title claims abstract description 65
- 230000027455 binding Effects 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 31
- 108020004414 DNA Proteins 0.000 claims abstract description 112
- 238000006243 chemical reaction Methods 0.000 claims abstract description 53
- 102000053602 DNA Human genes 0.000 claims abstract description 44
- 238000001962 electrophoresis Methods 0.000 claims abstract description 15
- 239000011541 reaction mixture Substances 0.000 claims abstract description 7
- 239000007795 chemical reaction product Substances 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 108091008146 restriction endonucleases Proteins 0.000 claims description 5
- 238000013508 migration Methods 0.000 claims description 4
- 230000005012 migration Effects 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000007481 next generation sequencing Methods 0.000 claims 1
- 238000011002 quantification Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 abstract description 8
- 206010028980 Neoplasm Diseases 0.000 description 13
- 201000011510 cancer Diseases 0.000 description 13
- 238000012163 sequencing technique Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 238000011156 evaluation Methods 0.000 description 7
- 238000011528 liquid biopsy Methods 0.000 description 6
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000030609 dephosphorylation Effects 0.000 description 2
- 238000006209 dephosphorylation reaction Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000000865 phosphorylative effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/125—Electrophoretic separation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020207319 | 2020-12-15 | ||
| JPJP-P-2020-207319 | 2020-12-15 | ||
| PCT/JP2021/046211 WO2022131285A1 (ja) | 2020-12-15 | 2021-12-15 | Ｄｎａサンプルのシーケンスにおけるアダプター結合効率を評価する方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20230121076A true KR20230121076A (ko) | 2023-08-17 |
Family
ID=82057603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020237021963A Pending KR20230121076A (ko) | 2020-12-15 | 2021-12-15 | Dna 샘플의 시퀀스에 있어서의 어댑터 결합 효율을평가하는 방법 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240102089A1 (https=) |
| EP (1) | EP4265721A4 (https=) |
| JP (1) | JPWO2022131285A1 (https=) |
| KR (1) | KR20230121076A (https=) |
| CN (1) | CN116615537A (https=) |
| AU (1) | AU2021401369A1 (https=) |
| CA (1) | CA3201748A1 (https=) |
| WO (1) | WO2022131285A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025117738A1 (en) * | 2023-11-28 | 2025-06-05 | Illumina, Inc. | Methods of improving unique molecular index ligation efficiency |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019504624A (ja) | 2016-01-29 | 2019-02-21 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 核酸配列決定のための新規アダプターおよび使用法 |
| JP6685324B2 (ja) | 2015-04-28 | 2020-04-22 | イラミーナ インコーポレーテッド | 特異的分子インデックス（ｕｍｉ）を有する冗長リードを用いたシーケンシングｄｎａ断片におけるエラーの抑制 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110382709A (zh) * | 2016-09-12 | 2019-10-25 | 维尔道应用技术大学 | 可转化的衔接子 |
| EP3885445B1 (en) * | 2017-04-14 | 2023-08-23 | Guardant Health, Inc. | Methods of attaching adapters to sample nucleic acids |
| US11447818B2 (en) * | 2017-09-15 | 2022-09-20 | Illumina, Inc. | Universal short adapters with variable length non-random unique molecular identifiers |
| AU2019207900B2 (en) * | 2018-01-12 | 2025-07-10 | Claret Bioscience, Llc | Methods and compositions for analyzing nucleic acid |
| EP4245861B1 (en) * | 2018-05-08 | 2025-02-26 | MGI Tech Co., Ltd. | Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly |
-
2021
- 2021-12-15 KR KR1020237021963A patent/KR20230121076A/ko active Pending
- 2021-12-15 AU AU2021401369A patent/AU2021401369A1/en not_active Abandoned
- 2021-12-15 CA CA3201748A patent/CA3201748A1/en active Pending
- 2021-12-15 CN CN202180083883.5A patent/CN116615537A/zh active Pending
- 2021-12-15 US US18/267,732 patent/US20240102089A1/en active Pending
- 2021-12-15 WO PCT/JP2021/046211 patent/WO2022131285A1/ja not_active Ceased
- 2021-12-15 JP JP2022570032A patent/JPWO2022131285A1/ja active Pending
- 2021-12-15 EP EP21906647.9A patent/EP4265721A4/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6685324B2 (ja) | 2015-04-28 | 2020-04-22 | イラミーナ インコーポレーテッド | 特異的分子インデックス（ｕｍｉ）を有する冗長リードを用いたシーケンシングｄｎａ断片におけるエラーの抑制 |
| JP2019504624A (ja) | 2016-01-29 | 2019-02-21 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 核酸配列決定のための新規アダプターおよび使用法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4265721A1 (en) | 2023-10-25 |
| CN116615537A (zh) | 2023-08-18 |
| CA3201748A1 (en) | 2022-06-23 |
| JPWO2022131285A1 (https=) | 2022-06-23 |
| AU2021401369A1 (en) | 2023-07-27 |
| US20240102089A1 (en) | 2024-03-28 |
| EP4265721A4 (en) | 2024-11-13 |
| WO2022131285A1 (ja) | 2022-06-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20230629 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20240920 Comment text: Request for Examination of Application |