CA3201748A1 - Method for evaluating adapter ligation efficiency in sequencing of dna sample - Google Patents
Method for evaluating adapter ligation efficiency in sequencing of dna sampleInfo
- Publication number
- CA3201748A1 CA3201748A1 CA3201748A CA3201748A CA3201748A1 CA 3201748 A1 CA3201748 A1 CA 3201748A1 CA 3201748 A CA3201748 A CA 3201748A CA 3201748 A CA3201748 A CA 3201748A CA 3201748 A1 CA3201748 A1 CA 3201748A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- adapter
- ligation
- double
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/125—Electrophoretic separation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020-207319 | 2020-12-15 | ||
| JP2020207319 | 2020-12-15 | ||
| PCT/JP2021/046211 WO2022131285A1 (ja) | 2020-12-15 | 2021-12-15 | Ｄｎａサンプルのシーケンスにおけるアダプター結合効率を評価する方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3201748A1 true CA3201748A1 (en) | 2022-06-23 |
Family
ID=82057603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3201748A Pending CA3201748A1 (en) | 2020-12-15 | 2021-12-15 | Method for evaluating adapter ligation efficiency in sequencing of dna sample |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240102089A1 (https=) |
| EP (1) | EP4265721A4 (https=) |
| JP (1) | JPWO2022131285A1 (https=) |
| KR (1) | KR20230121076A (https=) |
| CN (1) | CN116615537A (https=) |
| AU (1) | AU2021401369A1 (https=) |
| CA (1) | CA3201748A1 (https=) |
| WO (1) | WO2022131285A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025117738A1 (en) * | 2023-11-28 | 2025-06-05 | Illumina, Inc. | Methods of improving unique molecular index ligation efficiency |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10844428B2 (en) | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
| ES2924487T3 (es) | 2016-01-29 | 2022-10-07 | Hoffmann La Roche | Un adaptador de conformación en Y novedoso para secuenciación de ácidos nucleicos y procedimiento de uso |
| CN110382709A (zh) * | 2016-09-12 | 2019-10-25 | 维尔道应用技术大学 | 可转化的衔接子 |
| EP3885445B1 (en) * | 2017-04-14 | 2023-08-23 | Guardant Health, Inc. | Methods of attaching adapters to sample nucleic acids |
| US11447818B2 (en) * | 2017-09-15 | 2022-09-20 | Illumina, Inc. | Universal short adapters with variable length non-random unique molecular identifiers |
| AU2019207900B2 (en) * | 2018-01-12 | 2025-07-10 | Claret Bioscience, Llc | Methods and compositions for analyzing nucleic acid |
| EP4245861B1 (en) * | 2018-05-08 | 2025-02-26 | MGI Tech Co., Ltd. | Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly |
-
2021
- 2021-12-15 KR KR1020237021963A patent/KR20230121076A/ko active Pending
- 2021-12-15 AU AU2021401369A patent/AU2021401369A1/en not_active Abandoned
- 2021-12-15 CA CA3201748A patent/CA3201748A1/en active Pending
- 2021-12-15 CN CN202180083883.5A patent/CN116615537A/zh active Pending
- 2021-12-15 US US18/267,732 patent/US20240102089A1/en active Pending
- 2021-12-15 WO PCT/JP2021/046211 patent/WO2022131285A1/ja not_active Ceased
- 2021-12-15 JP JP2022570032A patent/JPWO2022131285A1/ja active Pending
- 2021-12-15 EP EP21906647.9A patent/EP4265721A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| KR20230121076A (ko) | 2023-08-17 |
| EP4265721A1 (en) | 2023-10-25 |
| CN116615537A (zh) | 2023-08-18 |
| JPWO2022131285A1 (https=) | 2022-06-23 |
| AU2021401369A1 (en) | 2023-07-27 |
| US20240102089A1 (en) | 2024-03-28 |
| EP4265721A4 (en) | 2024-11-13 |
| WO2022131285A1 (ja) | 2022-06-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD Year of fee payment: 3 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20240924 |
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| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT Effective date: 20240925 |