CN116615537A - 评价dna样品的测序中的适配体结合效率的方法 - Google Patents

评价dna样品的测序中的适配体结合效率的方法 Download PDF

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Publication number
CN116615537A
CN116615537A CN202180083883.5A CN202180083883A CN116615537A CN 116615537 A CN116615537 A CN 116615537A CN 202180083883 A CN202180083883 A CN 202180083883A CN 116615537 A CN116615537 A CN 116615537A
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China
Prior art keywords
dna
aptamer
reaction
sequencing
binding
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Pending
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CN202180083883.5A
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English (en)
Chinese (zh)
Inventor
田中正史
猪子英俊
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GENODIVE PHARMA Inc
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GENODIVE PHARMA Inc
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Publication of CN116615537A publication Critical patent/CN116615537A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/125—Electrophoretic separation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180083883.5A 2020-12-15 2021-12-15 评价dna样品的测序中的适配体结合效率的方法 Pending CN116615537A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-207319 2020-12-15
JP2020207319 2020-12-15
PCT/JP2021/046211 WO2022131285A1 (ja) 2020-12-15 2021-12-15 Ｄｎａサンプルのシーケンスにおけるアダプター結合効率を評価する方法

Publications (1)

Publication Number Publication Date
CN116615537A true CN116615537A (zh) 2023-08-18

Family

ID=82057603

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180083883.5A Pending CN116615537A (zh) 2020-12-15 2021-12-15 评价dna样品的测序中的适配体结合效率的方法

Country Status (8)

Country Link
US (1) US20240102089A1 (https=)
EP (1) EP4265721A4 (https=)
JP (1) JPWO2022131285A1 (https=)
KR (1) KR20230121076A (https=)
CN (1) CN116615537A (https=)
AU (1) AU2021401369A1 (https=)
CA (1) CA3201748A1 (https=)
WO (1) WO2022131285A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025117738A1 (en) * 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
ES2924487T3 (es) 2016-01-29 2022-10-07 Hoffmann La Roche Un adaptador de conformación en Y novedoso para secuenciación de ácidos nucleicos y procedimiento de uso
CN110382709A (zh) * 2016-09-12 2019-10-25 维尔道应用技术大学 可转化的衔接子
EP3885445B1 (en) * 2017-04-14 2023-08-23 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
US11447818B2 (en) * 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
AU2019207900B2 (en) * 2018-01-12 2025-07-10 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
EP4245861B1 (en) * 2018-05-08 2025-02-26 MGI Tech Co., Ltd. Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly

Also Published As

Publication number Publication date
KR20230121076A (ko) 2023-08-17
EP4265721A1 (en) 2023-10-25
CA3201748A1 (en) 2022-06-23
JPWO2022131285A1 (https=) 2022-06-23
AU2021401369A1 (en) 2023-07-27
US20240102089A1 (en) 2024-03-28
EP4265721A4 (en) 2024-11-13
WO2022131285A1 (ja) 2022-06-23

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