KR20230081979A - Composition for anti-inflammation comprising gold nanoparticle-peanut sprout extract as an active ingredient - Google Patents

Abstract

The present invention relates to an anti-inflammatory composition containing gold nano-peanut sprout extract as an active ingredient. The gold nano-peanut sprout extract according to the present invention exhibits effects of inhibiting NO production, inhibiting IL-6 production, inhibiting TNF-α production, and inhibiting ROS production, and thus has an anti-inflammatory effect. Therefore, it is expected to be usefully used as a pharmaceutical composition, food composition, or cosmetic composition having the above physiological activity.

Description

Composition for anti-inflammation comprising gold nanoparticle-peanut sprout extract as an active ingredient

It relates to an anti-inflammatory composition comprising a gold nano-peanut sprout extract as an active ingredient.

Skin, as a physical barrier that protects the body against various environmental factors, plays an important role in protecting internal organs from physical and chemical stimuli such as ultraviolet rays and maintaining homeostasis. The skin is composed of the epidermis, dermis, and subcutaneous fat. In particular, the epidermis of the skin is mostly composed of keratinocytes, which form keratin and produce various cytokines to respond to immune and inflammatory responses. known to be involved. When dermal keratinocytes are exposed to environmental and physiological factors, an inflammatory response occurs and various inflammatory cytokines and chemokines are secreted, which reduces the proliferation rate of keratinocytes and speeds up the recovery of damaged skin. Decrease. Therefore, proliferation of skin keratinocytes plays an important role in inflammatory skin diseases, and is closely related to skin diseases such as skin aging, atopic dermatitis, and psoriasis.

Inflammation is an essential response for the body to protect itself, but if not controlled it can lead to various diseases such as rheumatoid arthritis, atherosclerosis, and asthma. Macrophages, which play an important role in regulating innate and adaptive immune responses, remove pathogens by phagocytosis when pathogens pass through the epithelial barrier. Macrophages produce pro-inflammatory cytokines (TNF-α interleukin (IL)-6), chemokines (IL-8 and monocyte chemoattractant protein (MCP)-1), and inflammatory enzymes (iNOS2 (inducible It is involved in the immune response by producing inflammatory mediators including nitric oxide synthase) and cyclooxygenase (COX)-2). Lipopolysaccharide (LPS) is a substance that causes oxidative stress and is a kind of constituent material present in the cell wall of Gram-negative bacteria. It is known that it causes the secretion of cytokines due to the activation of the signal transduction process of macrophages or monocytes and induces the activity of the hypothalamus and adrenal cortex to generate NO. In particular, LPS amplifies the body's inflammatory response, and when macrophages are stimulated with LPS, a large amount of NO produced by the expression of iNOS serves as a mediator of the inflammatory response. Therefore, controlling inflammation and maintaining an appropriate level of the immune response are very important to prevent these diseases.

Peanuts are a representative high-fat, high-protein health food. It is native to the South American continent centered on Brazil, and it is estimated that it was introduced to Korea around 1780 (Jeongjo’s 4th year), and it is believed that full-scale cultivation took place after the flowering period. Peanut is a natural health food that is three times higher in lipid than general soybeans and rich in various nutrients such as 13 kinds of vitamins and 26 kinds of minerals. In the United States, about 56% of peanut consumption is used to make peanut butter, and the rest is roasted and used for snacks or confectionery. In Europe, peanuts are widely used for milking. In Korea, it is processed in the form of roasted peanuts, which are mainly roasted and eaten, and is widely used as a side dish or snack, and is used in various forms such as ingredients for confectionery and baking, favorite food, and health food for patients. Peanut sprouts refer to crops harvested within an appropriate period, for example, between 4 and 12 days after germination of peanuts using a culture medium.

Until now, there has been no research on gold nano-peanut sprout extract. Therefore, by verifying the anti-inflammatory effect of the gold nano-peanut sprout extract, it is intended to confirm its applicability as a food composition, pharmaceutical composition, and cosmetic composition.

Korean Patent Publication No. 10-2021-0073251

The present inventors confirmed the anti-inflammatory effect of the gold nano-peanut sprout extract, and based on this, the present invention was completed.

Accordingly, an object of the present invention is to provide an anti-inflammatory composition comprising gold nano-peanut sprout extract as an active ingredient.

In addition, another object of the present invention is to provide a pharmaceutical composition for preventing or treating inflammatory diseases, comprising a gold nano-peanut sprout extract as an active ingredient.

In addition, another object of the present invention is to provide a cosmetic composition for preventing or improving inflammation, comprising a gold nano-peanut sprout extract as an active ingredient.

In addition, another object of the present invention is to provide a health functional food composition for preventing or improving inflammation, including gold nano-peanut sprout extract as an active ingredient.

In addition, another object of the present invention is a method for producing a gold nano-peanut sprout extract comprising the step of mixing a peanut sprout extract and a gold salt,

The concentration of the gold salt is 0.1 to 10 mM; and

The concentration of the peanut sprout extract is to provide a method for preparing a gold nano-peanut sprout extract, characterized in that 0.1 to 10mg / mL.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned can be clearly understood by those skilled in the art from the description below. There will be.

The terms used herein are used for descriptive purposes only and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

Hereinafter, the present invention will be described in more detail.

According to one aspect of the present invention, there is provided an anti-inflammatory composition comprising gold nano-peanut sprout extract as an active ingredient.

In the present invention, "peanuts ( Arachis hypogaea )" is an annual herbaceous plant belonging to the leguminous family. It is three times higher in lipid than general legumes and is a natural health food rich in various nutrients such as 13 kinds of vitamins and 26 kinds of minerals.

In the present invention, "peanut sprout (peanut sprout)" refers to crops sprouted by germinating peanuts. The peanut sprout extract may be characterized as being a peanut sprout whole extract, but is not limited thereto. The peanut sprouts can be used without limitation, such as directly grown or commercially available.

In the present invention, the gold nano-peanut sprout extract is characterized in that it is prepared by a manufacturing method comprising mixing the peanut sprout extract and gold salt, but is not limited thereto.

In the present invention, "extract" refers to an extract obtained by the extraction treatment of the peanut sprout, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, It includes the extract itself and extracts of all formulations that can be formed using the extract.

In the present invention, the extraction method of the peanut sprout extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include vacuum low temperature extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, solvent extraction, etc., which are alone It may be performed or a combination of two or more methods may be performed. According to one embodiment of the present invention, a vacuum low-temperature extraction method was used, but is not limited thereto.

In the present invention, the type of extraction solvent used to extract the peanut sprout extract is not particularly limited, and according to a conventional method known in the art for extracting an extract from natural products, that is, conventional temperature and pressure It can be extracted using conventional solvents under the conditions of For example, the peanut sprout extract is water, C ₁ to C ₄ lower alcohol, n-hexane, ethyl acetate, acetone, acetonitrile, butyl acetate, 1,3-butylene glycol, methylene chloride, and a mixture thereof It may be extracted with one solvent selected from the group consisting of solvents, and according to one embodiment of the present invention, it may be water, but is not limited thereto.

In the present invention, the prepared extract may then be filtered or concentrated or dried to remove the solvent, and both filtration, concentration and drying may be performed. For example, filtration may use filter paper or a vacuum filter, and concentration may use a vacuum vacuum concentrator or vacuum rotary evaporator, but is not limited thereto. In addition, drying may be performed by vacuum drying, vacuum drying, boiling drying, spray drying, freeze drying, etc., but is not limited thereto.

In the present invention, the number of extractions may be carried out one or more times, but as the extraction continues, the yield of the active ingredient significantly decreases, so it may not be economical to repeat the extraction five times or more. Accordingly, the number of extractions is preferably 1 to 5 times, but is not limited thereto.

In the present invention, the gold nano-peanut sprout extract is 0.1 to 500 ug / ml, 0.1 to 400 ug / ml, 0.1 to 300 ug / ml, 1 to 500 ug / ml, 1 to 400 ug / ml, 1 to 300 ug/ml, 5 to 500 ug/ml, 5 to 400 ug/ml, 5 to 300 ug/ml, 10 to 500 ug/ml, 10 to 400 ug/ml, 10 to 300 ug/ml, 50 to 500 It may be included in the composition at a concentration of ug/ml, 50 to 400 ug/ml, 50 to 300 ug/ml, or 50 to 200 ug/ml, but is not limited thereto.

In the present invention, the gold nano-peanut sprout extract may be characterized in that it satisfies one or more of the following characteristics, but is not limited thereto: (a) suppression of NO production; (b) inhibition of IL-6 production; (c) inhibition of TNF-α production; and (d) inhibition of ROS production.

In addition, the present invention provides a pharmaceutical composition for preventing or treating inflammatory diseases, comprising a gold nano-peanut sprout extract as an active ingredient.

In the present invention, “anti-inflammatory” refers to an action of suppressing or reducing inflammation, and may be used to include prevention, improvement, or therapeutic activity of inflammatory diseases. The inflammatory disease may be selected from the group consisting of inflammatory bowel disease, inflammatory collagen vascular disease, glomerulonephritis, inflammatory skin disease, sarcoidosis, retinitis, gastritis, hepatitis, enteritis, arthritis, pneumonia, pancreatitis, sepsis, cystitis, nephritis, and neuritis. It may, but is not limited thereto.

In the present invention, the inflammatory skin disease may be one or more selected from the group consisting of atopic dermatitis, contact dermatitis, eczema, and psoriasis, but is not limited thereto.

The pharmaceutical composition according to the present invention may further include suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions. The excipient may be, for example, one or more selected from the group consisting of a diluent, a binder, a disintegrant, a lubricant, an adsorbent, a moisturizer, a film-coating material, and a controlled release additive.

The pharmaceutical compositions according to the present invention are powders, granules, sustained-release granules, enteric granules, solutions, eye drops, elsilic agents, emulsions, suspensions, spirits, troches, perfumes, and limonadese, respectively, according to conventional methods. , tablets, sustained-release tablets, enteric tablets, sublingual tablets, hard capsules, soft capsules, sustained-release capsules, enteric capsules, pills, tinctures, soft extracts, dry extracts, fluid extracts, injections, capsules, perfusate, It can be formulated and used in the form of external preparations such as warning agents, lotions, pasta agents, sprays, inhalants, patches, sterile injection solutions, or aerosols, and the external agents are creams, gels, patches, sprays, ointments, and warning agents. , lotion, liniment, pasta, or cataplasma may have formulations such as the like.

Carriers, excipients and diluents that may be included in the pharmaceutical composition according to the present invention include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants.

Corn starch, potato starch, wheat starch, lactose, sucrose, glucose, fructose, di-mannitol, precipitated calcium carbonate, synthetic aluminum silicate, phosphoric acid as additives for tablets, powders, granules, capsules, pills, and troches according to the present invention Calcium monohydrogen, calcium sulfate, sodium chloride, sodium bicarbonate, purified lanolin, microcrystalline cellulose, dextrin, sodium alginate, methylcellulose, sodium carboxymethylcellulose, kaolin, urea, colloidal silica gel, hydroxypropyl starch, hydroxypropylmethyl Excipients such as cellulose (HPMC) 1928, HPMC 2208, HPMC 2906, HPMC 2910, propylene glycol, casein, calcium lactate, Primogel; Gelatin, gum arabic, ethanol, agar powder, cellulose phthalate acetate, carboxymethyl cellulose, calcium carboxymethyl cellulose, glucose, purified water, sodium caseinate, glycerin, stearic acid, sodium carboxymethyl cellulose, sodium methyl cellulose, methyl cellulose, microcrystalline cellulose, dextrin Binders such as hydroxycellulose, hydroxypropyl starch, hydroxymethylcellulose, purified shellac, starch arc, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinyl alcohol, and polyvinylpyrrolidone may be used, Hydroxypropyl Methyl Cellulose, Corn Starch, Agar Powder, Methyl Cellulose, Bentonite, Hydroxypropyl Starch, Sodium Carboxymethyl Cellulose, Sodium Alginate, Calcium Carboxymethyl Cellulose, Calcium Citrate, Sodium Lauryl Sulfate, Silicic Anhydride, 1-Hydroxy Propyl cellulose, dextran, ion exchange resin, polyvinyl acetate, formaldehyde-treated casein and gelatin, alginic acid, amylose, guar gum, sodium bicarbonate, polyvinylpyrrolidone, calcium phosphate, gelled starch, gum arabic, disintegrants such as amylopectin, pectin, sodium polyphosphate, ethyl cellulose, white sugar, magnesium aluminum silicate, di-sorbitol solution, and light anhydrous silicic acid; Calcium stearate, magnesium stearate, stearic acid, hydrogenated vegetable oil, talc, lycopod, kaolin, petrolatum, sodium stearate, cacao butter, sodium salicylate, magnesium salicylate, polyethylene glycol 4000, polyethylene glycol 6000, liquid paraffin, hydrogenated soybean oil (Lubri wax), aluminum stearate, zinc stearate, sodium lauryl sulfate, magnesium oxide, macrogol, synthetic aluminum silicate, silicic anhydride, higher fatty acid, higher alcohol, silicone oil, paraffin oil, polyethylene glycol fatty acid ether, starch, A lubricant such as sodium chloride, sodium acetate, sodium oleate, dl-leucine, light anhydrous silicic acid; may be used.

Additives for the liquid formulation according to the present invention include water, dilute hydrochloric acid, dilute sulfuric acid, sodium citrate, sucrose monostearate, polyoxyethylene sorbitol fatty acid esters (tween esters), polyoxyethylene monoalkyl ethers, lanolin ethers, Lanolin esters, acetic acid, hydrochloric acid, aqueous ammonia, ammonium carbonate, potassium hydroxide, sodium hydroxide, prolamine, polyvinylpyrrolidone, ethyl cellulose, sodium carboxymethyl cellulose, and the like may be used.

In the syrup according to the present invention, a solution of white sugar, other sugars, or a sweetener may be used, and aromatics, coloring agents, preservatives, stabilizers, suspending agents, emulsifiers, thickeners, etc. may be used as necessary.

Purified water may be used in the emulsion according to the present invention, and emulsifiers, preservatives, stabilizers, fragrances, etc. may be used as needed.

Suspension agents according to the present invention include acacia, tragacantha, methylcellulose, carboxymethylcellulose, sodium carboxymethylcellulose, microcrystalline cellulose, sodium alginate, hydroxypropylmethylcellulose (HPMC), HPMC 1828, HPMC 2906, HPMC 2910, etc. Agents may be used, and surfactants, preservatives, stabilizers, colorants, and fragrances may be used as needed.

Injections according to the present invention include distilled water for injection, 0.9% sodium chloride injection, IV injection, dextrose injection, dextrose + sodium chloride injection, PEG, lactated IV injection, ethanol, propylene glycol, non-volatile oil-sesame oil , solvents such as cottonseed oil, peanut oil, soybean oil, corn oil, ethyl oleate, isopropyl myristate, and benzene benzoate; solubilizing agents such as sodium benzoate, sodium salicylate, sodium acetate, urea, urethane, monoethylacetamide, butazolidine, propylene glycol, twins, nijuntinamide, hexamine, and dimethylacetamide; buffers such as weak acids and their salts (acetic acid and sodium acetate), weak bases and their salts (ammonia and ammonium acetate), organic compounds, proteins, albumin, peptone, and gums; tonicity agents such as sodium chloride; Stabilizers such as sodium bisulfite (NaHSO ₃ ), carbon dioxide gas, sodium metabisulfite (Na ₂ S ₂ O ₅ ), sodium sulfite (Na ₂ SO ₃ ), nitrogen gas (N ₂ ), and ethylenediaminetetraacetic acid; Sulfating agents such as sodium bisulfide 0.1%, sodium formaldehyde sulfoxylate, thiourea, ethylenediamine disodium tetraacetate, acetone sodium bisulfite; analgesics such as benzyl alcohol, chlorobutanol, procaine hydrochloride, glucose, and calcium gluconate; Suspending agents such as Siemesis sodium, sodium alginate, Tween 80, aluminum monostearate may be included.

The suppository according to the present invention includes cacao butter, lanolin, witapsol, polyethylene glycol, glycerogelatin, methylcellulose, carboxymethylcellulose, a mixture of stearic acid and oleic acid, subanal, cottonseed oil, peanut oil, palm oil, cacao butter + Cholesterol, Lecithin, Lannet Wax, Glycerol Monostearate, Tween or Span, Imhausen, Monolen (Propylene Glycol Monostearate), Glycerin, Adeps Solidus, Buytyrum Tego-G -G), Cebes Pharma 16, Hexalide Base 95, Cotomar, Hydroxycote SP, S-70-XXA, S-70-XX75 (S-70-XX95), Hyde Hydrokote 25, Hydrokote 711, Idropostal, Massa estrarium (A, AS, B, C, D, E, I, T), Massa-MF, Masupol, Masupol-15, Neosupostal-N, Paramound-B, Suposiro (OSI, OSIX, A, B, C, D, H, L), suppository type IV (AB, B, A, BC, BBG, E, BGF, C, D, 299), Supostal (N, Es), Wecobi (W, R, S, M, Fs), testosterone triglyceride base (TG-95, MA, 57) and The same mechanism can be used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, for example, starch, calcium carbonate, sucrose, etc. ) or by mixing lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used.

Liquid preparations for oral administration include suspensions, solutions for oral administration, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. there is. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is the type of patient's disease, severity, activity of the drug, It may be determined according to factors including sensitivity to the drug, administration time, route of administration and excretion rate, duration of treatment, drugs used concurrently, and other factors well known in the medical field.

The pharmaceutical composition according to the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered single or multiple times. Considering all of the above factors, it is important to administer an amount that can obtain the maximum effect with the minimum amount without side effects, which can be easily determined by a person skilled in the art to which the present invention belongs.

The pharmaceutical composition of the present invention can be administered to a subject by various routes. All modes of administration can be envisaged, eg oral administration, subcutaneous injection, intraperitoneal administration, intravenous injection, intramuscular injection, paraspinal space (intrathecal) injection, sublingual administration, buccal administration, intrarectal insertion, vaginal It can be administered by intraoral insertion, ocular administration, otic administration, nasal administration, inhalation, spraying through the mouth or nose, dermal administration, transdermal administration, and the like.

The pharmaceutical composition of the present invention is determined according to the type of drug as an active ingredient together with various related factors such as the disease to be treated, the route of administration, the age, sex, weight and severity of the disease of the patient.

In the present invention, "individual" means a subject in need of treatment of a disease, and more specifically, a human or non-human primate, mouse, rat, dog, cat, horse, cow, etc. of mammals.

In the present invention, "administration" means providing a given composition of the present invention to a subject by any suitable method.

In the present invention, “prevention” refers to any action that suppresses or delays the onset of a desired disease, and “treatment” means that the desired disease and its resulting metabolic abnormality are improved or improved by administration of the pharmaceutical composition according to the present invention. All actions that are advantageously altered are meant, and "improvement" means any action that reduces the parameters related to the target disease, for example, the severity of symptoms, by administration of the composition according to the present invention.

In addition, the present invention provides a cosmetic composition for preventing or improving inflammation, comprising a gold nano-peanut sprout extract as an active ingredient.

The formulation of the cosmetic composition according to the present invention is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, mist, moisture cream, hand cream, hand lotion, foundation, It may be in the form of essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, cleansing oil, cleansing balm, body lotion, or body cleanser.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, and sphingolipids.

Any water-soluble vitamin can be used as long as it can be incorporated into cosmetics, but examples include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamin H. salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium salt of ascorbic acid-2-phosphate, etc.) included Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method, or a chemical synthesis method.

Any useful vitamin can be used as long as it can be formulated into cosmetics, but examples thereof include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol), and their derivatives (palmitic acid). Ascorbine, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) Included in the useful vitamins used in the invention. The useful vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial culture, enzymatic or chemical synthesis.

Any polymer peptide may be used as long as it can be incorporated into cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymer peptides can be purified and obtained by conventional methods such as a purification method from a culture medium of microorganisms, an enzyme method, or a chemical synthesis method, or can be used after being purified from natural products such as pig or cow dermis or silkworm silk fibers.

The polymeric polysaccharide may be any material as long as it can be incorporated into cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or salts thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

Any sphingolipid can be used as long as it can be incorporated into cosmetics, and examples thereof include ceramide, phytosphingosine, and sphingoglycolipid. Sphingolipids can be purified by conventional methods or obtained by chemical synthesis from mammals, fish, shellfish, yeast, or plants.

In the cosmetic composition of the present invention, in addition to the above essential ingredients, other ingredients normally formulated in cosmetics may be blended as necessary.

Other ingredients that may be added include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, A blood circulation accelerator, a cooling agent, an antiperspirant, purified water, etc. are mentioned.

Examples of the oil and fat component include ester oils, hydrocarbon oils, silicone oils, fluorine oils, animal fats and vegetable oils.

Examples of ester oils include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelislitol tetra2-ethylhexanoate , Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostea laurate Lil, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate Aryl, 2-ethyl stearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di(caprylic, capric acid) propylene glycol, propylene glycol dicaprylate, dicap Neopentyl glycol prinate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , Octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, dimalate Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, Cholesteryl Hydroxystearate, Cholesteryl Oleate, Dihydrocholesteryl Oleate, Physteryl Isostearate, Physteryl Oleate, Isocetyl 12-stealoylhydroxystearate, 12-stealoyylhydro and esters such as stearyl oxystearate and isostearyl 12-stealoylhydroxystearate.

Examples of hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and vaseline.

Examples of silicone oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealoxysiloxane copolymer, alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

Examples of fluorine-based fats and oils include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, algae oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil , cottonseed oil, palm oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, moonshine colostrum, marker damian nut oil, meadowsweet oil, egg yolk oil, beef tallow, horse oil, mink oil, orange raffia oil, jojoba oil , animal or plant fats and oils such as candelilla wax, carnaba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizer include water-soluble low-molecular moisturizers, fat-soluble molecular moisturizers, water-soluble polymers, and oil-soluble polymers.

Examples of water-soluble low-molecular moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactate, etc. are mentioned.

Cholesterol, cholesterol ester, etc. are mentioned as a fat-soluble low-molecular moisturizer.

Examples of water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, and the like. can

Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high molecular silicone.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE·POP (polyoxyethylene·polyoxypropylene) copolymer, POE·POP alkyl ether, polyether modified silicone, lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkyl amide sulfates, alkyl phosphates, POE alkyl phosphates, and alkyl amide phosphates. , alkyloylalkyl taurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinic acid salts, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. .

As the cationic surfactant, alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, chloride Benzalkonium, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salt, etc. are mentioned.

Amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. An amphoteric surfactant etc. are mentioned.

As organic and inorganic pigments, silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenolic resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, organic pigments such as silk powder, cellulose, CI pigment yellow and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts, such as zinc sodium cetylrate, zinc laurylrate, and calcium laurylrate; polyvalent metal salts of acyl amino acids such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; polyvalent metal salts of amide sulfonic acids such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hardened beef fatty acid acylarginine, etc. -acyl basic amino acids; N-acyl polypeptides such as N-lauroylglycylglycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, tetrafluoroethylene, and the like.

Examples of UV absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, and benzyl cinnamate. , paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl-diisopropylcinnamic acid ester mixture, uro Cannic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone , tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1 ,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Bactericides include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincophylthione, benzalkonium chloride, photosensitizer So No. 301, mononitroguacol sodium, undecyrenic acid, etc. are mentioned.

Examples of antioxidants include butylhydroxyanisole, propyl gallic acid, and elisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, and sodium monohydrogenphosphate.

Examples of alcohol include higher alcohols such as cetyl alcohol.

In addition, the blending components that may be added are not limited to these, and any of the above ingredients can be blended within a range not impairing the objects and effects of the present invention, but 0.01 to 5% by weight percent or 0.01 to 3% based on the total weight. Can be formulated in % weight percentage.

When the formulation of the present invention is a lotion, paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component can be used

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

In addition, it provides a health functional food composition for preventing or improving inflammation, including gold nano-peanut sprout extract as an active ingredient.

In the present invention, "food" means a natural product or processed product containing one or more nutrients, preferably means a state that can be eaten directly through a certain degree of processing process, and usually means As, it means to include all health functional foods, beverages, food additives and beverage additives.

In the present invention, the "functional food" is the same term as food for special health use (FoSHU), medicine processed to efficiently display bioregulatory functions in addition to nutritional supply, It means a food with high medical effect, and can be manufactured into tablets, capsules, pills, granules, powders, liquids, flakes, pastes, syrups, gels, jellies, bars, or film formulations. Here, "function" means obtaining useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions.

When the gold nano-peanut sprout extract of the present invention is used as a food additive, it can be added as it is or used together with other foods or food ingredients, and can be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the gold nano-peanut sprout extract of the present invention may be added in an amount of 15% by weight or less, or 10% by weight or less based on the raw material. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

There is no particular limitation on the type of food. Examples of foods to which the above substances can be added include meat, sausages, bread, chocolates, candies, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice creams, various soups, beverages, tea, drinks, There are alcoholic beverages and vitamin complexes, and includes all health functional foods in a conventional sense.

The health beverage composition according to the present invention may contain various flavoring agents or natural carbohydrates as additional components, like conventional beverages. The aforementioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrins and cyclodextrins, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01-0.20 g, or about 0.04-0.10 g per 100 mL of the composition of the present invention.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, A carbonation agent used in carbonated beverages and the like may be contained. In addition, the composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not critical, but is generally selected in the range of 0.01-0.20 parts by weight per 100 parts by weight of the composition of the present invention.

The health functional food may have an advantage of having a more excellent effect by ingesting it in the form of inner beauty food. The inner beauty refers to food referred to as 'eating cosmetics or beauty food', which absorbs various ingredients good for the skin into the body and changes the skin constitution to a healthy one. You can select and consume the inner beauty food that suits your individual skin condition and lifestyle. For example, when cosmetics containing the cosmetic composition and inner beauty food containing the gold nano-peanut sprout extract are mixed, the effect is significantly higher than that of using only cosmetics or drugs, resulting in a more effective anti-inflammatory effect. may have advantages.

In addition, the present invention is a gold nano-peanut sprout extract manufacturing method comprising the step of mixing the peanut sprout extract and gold salt,

The concentration of the gold salt is 0.1 to 10 mM; and

The concentration of the peanut sprout extract is characterized in that 0.1 to 10mg / mL, gold nano-provides a method for producing peanut sprout extract.

In the present invention, the gold salt is 0.1 to 10mM, 0.1 to 8mM, 0.1 to 6mM, 0.1 to 4mM, 0.5 to 10mM, 0.5 to 8mM, 0.5 to 6mM, 0.5 to 4mM, 1 to 10mM, 1 to 8mM, 1 to 6 mM, 1 to 4 mM, 1 to 3 mM, or 2 mM gold nano-concentration may be included in the preparation of peanut sprout extract, but is not limited thereto.

In the present invention, the peanut sprout extract is 0.1 to 10mg / mL, 0.1 to 8mg / mL, 0.1 to 6mg / mL, 0.1 to 4mg / mL, 0.5 to 10mg / mL, 0.5 to 8mg / mL, 0.5 to 6mg / mL Gold nano-peanut sprout extract at a concentration of 0.5 to 4 mg/mL, 1 to 10 mg/mL, 1 to 8 mg/mL, 1 to 6 mg/mL, 1 to 4 mg/mL, 1 to 3 mg/mL, or 2 mg/mL It may be included in manufacturing, but is not limited thereto.

In the present invention, the mixing temperature may be 50 to 100 ℃, 50 to 90 ℃, 50 to 80 ℃, 60 to 100 ℃, 60 to 90 ℃, 60 to 80 ℃, 65 to 75 ℃, or 70 ℃ , but not limited thereto.

In the present invention, the mixing time may be 5 to 50 minutes, 5 to 40 minutes, 5 to 30 minutes, 10 to 50 minutes, 10 to 40 minutes, 10 to 30 minutes, 15 to 25 minutes, or 20 minutes, Not limited to this.

In the present invention, the gold nano-peanut sprout extract prepared by the above preparation method may be for anti-inflammatory use, but is not limited thereto.

The gold nano-peanut sprout extract according to the present invention exhibits effects of inhibiting NO production, IL-6 production, TNF-α production, and ROS production, and thus has an anti-inflammatory effect. Therefore, it is expected that it can be usefully used as a pharmaceutical composition, food composition, or cosmetic composition having the above physiological activity.

1 shows the results of confirming the optimal gold salt concentration (FIG. 1a), concentration of peanut sprout extract (FIG. 1b), temperature (FIG. 1c), and time (FIG. 1d) in preparing gold nano-peanut sprout extract. it is shown
Figure 2 shows the results of confirming the cytotoxicity according to the concentration of peanut sprout extract and gold nano-peanut sprout extract in RAW264.7 cells.
Figure 3 shows the results confirming the NO (nitric oxide) inhibitory effect according to the concentration of peanut sprout extract and gold nano-peanut sprout extract in RAW264.7 cells.
Figure 4 shows the result of confirming the IL-6 inhibitory effect according to the concentration of gold nano-peanut sprout extract in RAW264.7 cells.
Figure 5 shows the results of confirming the TNF-α inhibitory effect according to the concentration of gold nano-peanut sprout extract in RAW264.7 cells.
Figure 6 shows the results of confirming the ROS inhibitory effect according to the concentration of gold nano-peanut sprout extract in RAW264.7 cells.

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are provided to more easily understand the present invention, and the content of the present invention is not limited by the following examples.

[ Example ]

Example One. Peanut Sprout extract and gold nano - Peanut Sprout Extract preparation method

The extract was extracted from peanut sprout outpost using a vacuum low-temperature extraction process. When the external temperature is set to 120 ° C with a double jacket as a heat medium in dried peanut sprouts, the temperature is set so that the internal temperature of the reduced pressure is extracted at 60 ° C, and the vacuum is set to cool and condense the evaporated water vapor in the internal vacuum state to obtain an extract As a process, a peanut sprout extract having excellent physiological activity of a small molecule was secured. In the course of the experiment, water was used as the extraction solvent.

Optimal conditions were established using the following method to prepare gold nano-peanut sprout extract by combining gold nanoparticles with peanut sprout extract. First, gold salt (Sigma-Aldrich Gold(III) chloride trihydrate (HAuCl ₄ 3H ₂ O), CAS number 16961-25-4) was added at various concentrations of 0.5 to 3 mM to 1 mg/mL peanut sprout extract. and treated at room temperature for 20 minutes. When the gold nanoparticles are made, the absorbance is measured at 300 to 800 nm because the maximum absorption wavelength is shown at 400 to 600 nm. The results are shown in Figure 1a.

As shown in FIG. 1A, the prepared gold nano-peanut sprout extract exhibited a maximum absorbance value at 546 nm, and in particular, the highest absorbance value at a concentration of 2 mM gold salt.

Based on this, peanut sprout extract was added at a concentration of 1 to 5 mg/mL to a concentration of 2 mM gold salt and treated at room temperature for 20 minutes. The results are shown in Figure 1b.

As shown in Figure 1b, the highest absorbance value was shown at 2 mg/mL of peanut sprout extract. Therefore, it means that gold nanoparticles are best produced at a concentration of 2 mg/mL of peanut sprout extract and 2 mM of gold salt.

Thereafter, the absorbance was measured by setting the treatment time to 10 minutes, 20 minutes, and 30 minutes at 60 ° C, 70 ° C, 80 ° C, and 90 ° C in order to set the optimal temperature and optimal time. The results are shown in Figures 1c and 1d.

As shown in FIGS. 1c and 1d, the highest absorbance value was exhibited at 70° C., and the highest absorbance value was obtained at 20 minutes of treatment time.

Thereafter, centrifugation was performed at 13,000 rpm at 4° C. for 20 minutes to separate pure gold nano-peanut sprout extracts made under optimal conditions. Thereafter, the supernatant was removed and the pellet was washed with distilled water, followed by centrifugation in the same manner. This was repeated twice. 1mL of distilled water was added to the recovered pellet to dissolve it, and stored at 4℃ for use in future experiments.

Through the above results, it was confirmed that adding 2 mM gold salt to 2 mg/mL peanut sprout extract and treating it at 70° C. for 20 minutes is the optimal gold nano-method for preparing peanut sprout extract.

Example 2. gold nano - Peanut Sprout Confirmation of the anti-inflammatory effect of the extract

2.1. RAW264 .7 Confirmation of cytotoxicity to cells

The cytotoxicity of the samples of peanut sprout extract (PS extract) and gold nano-peanut sprout extract (PS AuNPs) against macrophage cell line Raw 264.7 cells was confirmed. Specifically, Raw 264.7 cells were dispensed in a 96 well culture plate at a concentration of 3×10 ⁵ cells/mL, and cultured for 12 hours to allow the cells to adhere to the plate. Thereafter, LPS 1 μg / mL and peanut sprout extract or gold nano-peanut sprout extract were simultaneously treated at various concentrations (3.125 to 100 μg / mL) and cultured for 24 hours, and then the supernatant was removed to measure cytotoxicity. Then, 100 μL of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) reagent was added and reacted for 180 minutes in a 37°C, 5% CO ₂ incubator and absorbance at 570 nm was measured. The results are shown in Figure 2.

As shown in Figure 2, it was confirmed that the peanut sprout extract and the gold nano-peanut sprout extract did not exhibit direct cytotoxicity at all concentrations.

2.2. gold nano - Peanut Sprout Nitric oxide (NO) in the extract ability to produce Confirmation of inhibitory effect

The ability of the gold nano-peanut sprout extract to produce NO, an inflammatory response mediator, was confirmed for macrophage cell line Raw 264.7 cells. Specifically, Raw 264.7 cells were dispensed in a 96-well culture plate at a concentration of 3×10 ⁵ cells/mL and cultured for 12 hours to attach the cells to the plate. LPS 1 μg/mL and gold nano-peanut sprout extract or peanut sprout extract were simultaneously treated at various concentrations (3.125 to 100 μg/mL) and cultured for 24 hours. After the end of the culture, 50 μL of the cell culture supernatant was recovered by centrifugation at 900 rpm for 5 minutes. Then, 50 μL of Griess Reagent was added and reacted for 30 minutes in a 37° C., 5% CO ₂ incubator, and the content of induced secreted NO in the culture supernatant was measured at 570 nm. The results are shown in Figure 3.

As shown in Figure 3, the peanut sprout extract did not inhibit the NO production ability. On the other hand, since the gold nano-peanut sprout extract inhibits NO production in a concentration-dependent manner, it can be confirmed that the gold nano-peanut sprout extract has an excellent anti-inflammatory effect.

2.3. gold nano - Peanut Sprout Confirmation of the IL-6 inhibitory effect of the extract

The ability of the gold nano-peanut sprout extract to produce IL-6, an anti-inflammatory cytokine, was confirmed. Specifically, Raw 264.7 cells were dispensed in a 96 well culture plate at a concentration of 3×10 ⁵ cells/mL and cultured for 12 hours to allow the cells to adhere to the plate. Then, 100 μg/mL of peanut sprout extract and gold nano-peanut sprout extract were treated with various concentrations (25 to 100 μg/mL) and incubated for 1 hour. Thereafter, 20 μL of TNF-α/IFN-γ was treated and cultured for 24 hours. After the end of the culture, centrifugation was performed at 900 rpm for 5 minutes, and 100 μL of the cell culture supernatant diluted 5 times was recovered. Then, the IL-6 ELISA kit was measured according to the instructions of the manufacturer (BD bioscience). The results are shown in FIG. 4 .

As shown in FIG. 4, the gold nano-peanut sprout extract decreased the production of IL-6 in a concentration-dependent manner, and in particular, at a concentration of 100 μg/mL, inhibition was superior to that of dexamethasone (DE), a positive control (PC). As the effect was shown, the excellent anti-inflammatory effect of the gold nano-peanut sprout extract was confirmed.

2.4. gold nano - Peanut Sprout of the extract TNF -α inhibitory effect confirmed

The TNF-α inhibitory effect of the gold nano-peanut sprout extract was confirmed. Specifically, the secretion level of TNF-α was estimated using an ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. RAW 264.7 macrophages (1 x 10 ⁴ cells/well) were seeded in a 96-well plate, treated with gold nano-peanut sprout extract, and then stimulated with 1 μg/ml LPS for 24 hours. Thereafter, the supernatant was collected and measured with a commercially available ELIZA kit to determine the level of these cytokines. The results are shown in FIG. 5 .

As shown in FIG. 5, the gold nano-peanut sprout extract inhibited TNF-α in a concentration-dependent manner, and in particular, at a concentration of 100 μg/mL, it showed a superior inhibitory effect than the positive control (PC), DE. , It confirmed the excellent anti-inflammatory effect of gold nano-peanut sprout extract.

2.5. gold nano - Peanut Sprout of the extract ROS Confirmation of inhibitory effect

The ROS inhibitory effect of the gold nano-peanut sprout extract was confirmed. Specifically, after treatment with the gold nano-peanut sprout extract for 24 hours, the cells were incubated with oxidative stress and superoxide detection reagents at 37° C. for 30 minutes. Cellular ROS/(Mito-SOX) Detection Assay Kit (Abcam, Cambridge, UK) with oxidative stress (green) and superoxide detection reagents was used to detect intracellular reactive oxygen species (ROS) release and mitochondrial superoxide (Mito-SOX) SOX, Abcam, Cambridge, UK (red) was detected. Fluorescence was measured using LSM 510 and 510 META laser scanning microscopes (Leica, Wetzlar, Germany). The results are shown in FIG. 6 .

As shown in FIG. 6, the gold nano-peanut sprout extract inhibited ROS in a concentration-dependent manner, and in particular, at a concentration of 100 μg/mL, it showed a better inhibitory effect than the positive control (PC), DE. It confirmed the excellent anti-inflammatory effect of the nano-peanut sprout extract.

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (12)

An anti-inflammatory composition comprising gold nano-peanut sprout extract as an active ingredient.
According to claim 1,
The composition, characterized in that the extract satisfies any one or more of the following characteristics:
(a) inhibition of NO production;
(b) inhibition of IL-6 production;
(c) inhibition of TNF-α production; and
(d) inhibition of ROS production.
A pharmaceutical composition for preventing or treating inflammatory diseases, comprising gold nano-peanut sprout extract as an active ingredient.
According to claim 3,
The inflammatory disease is selected from the group consisting of inflammatory bowel disease, inflammatory collagen vascular disease, glomerulonephritis, inflammatory skin disease, sarcoidosis, retinitis, gastritis, hepatitis, enteritis, arthritis, pneumonia, pancreatitis, sepsis, cystitis, nephritis, and neuritis. Characterized by the composition.
According to claim 4,
The inflammatory skin disease is characterized in that at least one selected from the group consisting of atopic dermatitis, contact dermatitis, eczema, and psoriasis, composition.
According to claim 3,
The peanut sprout extract is characterized in that extracted by a vacuum cold extraction method, composition.
A cosmetic composition for preventing or improving inflammation, comprising gold nano-peanut sprout extract as an active ingredient.
A health functional food composition for preventing or improving inflammation, comprising gold nano-peanut sprout extract as an active ingredient.
A method for preparing a gold nano-peanut sprout extract comprising mixing peanut sprout extract and gold salt,
The concentration of the gold salt is 0.1 to 10 mM; and
Characterized in that the concentration of the peanut sprout extract is 0.1 to 10mg / mL, gold nano-peanut sprout extract manufacturing method.
According to claim 9,
The mixing temperature is characterized in that 50 to 100 ℃, manufacturing method.
According to claim 9,
Characterized in that the mixing time is 5 to 50 minutes, the manufacturing method.
According to claim 9,
The gold nano-peanut sprout extract is characterized in that for anti-inflammatory, manufacturing method.

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