KR20230090109A - Cosmetic composition for anti-oxidation, anti-inflammation, pore tightening, or anti-wrinkle comprising extract of Delphinium grandiflorum L. as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for anti-oxidation, anti-inflammation, pore-tightening, or wrinkle prevention and improvement containing an extract of Delphinium grandiflorum L. as an active ingredient. The extract of Delphinium grandiflorum L. according to the present invention has an antioxidant effect by inhibiting DPPH radicals, ABTS radicals, and TBARs and increasing PF, and has an anti-inflammatory effect by inhibiting hyaluronidase. In addition, the extract of Delphinium grandiflorum L. has a wrinkle improvement effect by inhibiting elastase or collagenase and has an astringent effect to show a pore-tightening effect, thereby being expected to be usefully used for a pharmaceutical composition, a food composition, or a cosmetic composition having the above physiological activity.

Description

Cosmetic composition for anti-oxidation, anti-inflammation, pore tightening, or anti-wrinkle for anti-oxidation, anti-inflammation, pore reduction, or wrinkle prevention and improvement containing delphinium grandiflorum L. extract as an active ingredient extract of Delphinium grandiflorum L. as an active ingredient}

It relates to a cosmetic composition for anti-oxidation, anti-inflammatory, pore reduction, or wrinkle prevention and improvement containing Delphinium grandiflorum L. extract as an active ingredient.

With the development of society and technology, the problem of food has been solved and the treatment of diseases has become possible, so the average life span of humans has increased. As a result, as we entered the age of 100, the concept of ‘well-being’, that is, how to live better, was born, and interest in the cosmetics industry for the purpose of self-satisfaction or anti-aging increased. This degree of interest has evolved into ‘functional cosmetics’ that include specific intentions or purposes in relation to health, beyond simply looking pretty. As the functional cosmetics market expands, research on functional materials through various natural products, especially plants, has increased. Plants produce secondary metabolites called phytochemicals to protect themselves from predators, which can act as substances that help suppress cell damage and improve immune function in humans. For example, there are phenolic compounds, polyphenols and the like. Phenolic compounds include a wide range of substances that have an aromatic ring with one or more hydroxyl substituents. Since these natural materials of plants are excellent in safety, they have been spotlighted as functional materials and many studies have been conducted so far.

Fundamentally, the purpose of functional cosmetics is to prevent aging of the skin. Since aging is caused by oxidation of the skin and reduction of skin constituents, functional cosmetic materials increase skin constituents such as hyaluronic acid and collagen or inhibit melanin synthesis, resulting in skin elasticity. It should be able to give restoration and whitening effect. In addition, as an antioxidant, it prevents the progression of oxidation to prevent aging of the skin. Antioxidation is to prevent cell oxidation by removing free radicals (ROS) that are harmful to the human body. Active oxygen is a conversion of the remaining oxygen after entering the body and reacting, and attacks normal cells in the body, causing various diseases and aging. There is a defense system that protects against such damage in the human body, and SOD (superoxide dismutase), an antioxidant enzyme present in the body, is involved in this defense system. Since the ability of the enzyme decreases with increasing age, antioxidants must be ingested from the outside. Therefore, the development and research of new natural antioxidants having excellent antioxidant activity are continuously required and have been actively conducted.

Antioxidants are especially high in colored foods such as fruits and vegetables with strong colors. There are flavonoids as antioxidants present in plants. Flavonoids are heterocyclic compounds that exist in various natural products and have various physiological activities such as anti-cancer, anti-inflammatory, anti-viral, and anti-bacterial properties. Flavonoids derived from natural products have high stability due to long clinical experience and structure, and research on drugs such as whitening cosmetic compositions and malignant melanoma treatments through tyrosinase inhibitory effect and pigmentation inhibition has been reported.

Delphinium grandiflorum is a perennial herbaceous wildflower that can be found in meadows or foothills of 2,900m high mountains in the genus Delphinium of Ranunculaceae . Flowers are an important honey resource for bumblebees and hummingbirds, and are also distributed in the northern regions of Korea, Manchuria and Heilongjiang in China. The flowering period is about July 17 on average in summer, and in July-August, 3-15 flowers (bisexual) bloom at the end of the stem in a raceme, and the flowers are purple to indigo blue because they contain delphinidin pigment. The stem reaches 30~60cm, has an upright shape, and has short, curved, soft hairs. Two petals are sky blue, hairless, and the upper part is round, and the stamen is distorted spatula-shaped sky blue. Five calyx pieces are like petals, and there is a nectar bag at the top, and petals are contained in it. The fruit is a bone stone, 1.4-1.9cm long, straight, with short hairs on the surface, ripening in September-October. It has been confirmed through the National Institute of Biological Resources that records have been used for medicinal purposes such as stomach ailments, convulsions, and paralysis since ancient times. Until now, studies on swallowtails have been conducted on the inhibitory effect of bone loss due to osteoporosis related to cell proliferation and cell differentiation. etc. have been reported, but additional studies related to physiological activity are needed to confirm the functionality using only delphinium flowers.

Therefore, the present inventors pay attention to the physiological activity of the delphinium extract having blue pigment, and verify the antioxidation, anti-inflammatory, pore reduction, wrinkle prevention and improvement effects to confirm the applicability as a functional cosmetic material.

Korean Registered Patent No. 10-0767974

The present inventors confirmed the antioxidant, anti-inflammatory, pore reduction, wrinkle prevention and improvement effects of the delphinium extract, and based on this, the present invention was completed.

Accordingly, an object of the present invention is for antioxidant, containing an extract of Delphinium grandiflorum L. as an active ingredient; for anti-inflammatory use; for pore reduction; Or to provide a cosmetic composition for preventing and improving wrinkles.

Another object of the present invention is a delphinium ( Delphinium grandiflorum L. ) For antioxidant, containing an extract as an active ingredient; for anti-inflammatory use; for pore reduction; Or to provide a food composition for preventing and improving wrinkles.

Another object of the present invention is to provide a pharmaceutical composition for antioxidant or anti-inflammatory, containing an extract of Delphinium grandiflorum L. as an active ingredient.

Another object of the present invention is to provide a quasi-drug composition for anti-oxidation or anti-inflammatory, containing Delphinium grandiflorum L. as an active ingredient.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned can be clearly understood by those skilled in the art from the description below. There will be.

In order to achieve the object of the present invention, the present invention is a delphinium ( Delphinium grandiflorum L. ) For antioxidant containing an extract as an active ingredient; for anti-inflammatory use; for pore reduction; Or it provides a cosmetic composition for preventing and improving wrinkles.

In addition, the present invention is a delphinium ( Delphinium grandiflorum L. ) For antioxidant, containing an extract as an active ingredient; for anti-inflammatory use; for pore reduction; Or it provides a food composition for preventing and improving wrinkles.

In addition, the present invention provides a pharmaceutical composition for antioxidant or anti-inflammatory, containing an extract of Delphinium grandiflorum L. as an active ingredient.

In addition, the present invention provides a quasi-drug composition for anti-oxidation or anti-inflammatory, containing Delphinium grandiflorum L. extract as an active ingredient.

In one embodiment of the present invention, the delphinium extract may be characterized in that it is a delphinium flower extract, but is not limited thereto.

In another embodiment of the present invention, the delphinium extract is DPPH (1,1-diphenyl-2-picrylhydrazyl) radical inhibition, ABTS (2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) radical inhibition, TBARs (Thiobarbituric acid reactive substances) inhibition or PF (Antioxidant protection factor) may be characterized by having an increasing activity, but is not limited thereto.

In another embodiment of the present invention, the delphinium extract may be characterized as satisfying one or more of the following characteristics, but is not limited thereto:

(a) inhibit hyaluronidase;

(b) exhibits astringent action; and

(c) inhibition of elastase or collagenase.

In another embodiment of the present invention, the delphinium extract is water, C ₁ to C ₄ lower alcohol, n-hexane, ethyl acetate, acetone, acetonitrile, butyl acetate, 1,3-butylene glycol, methylene chloride, And it may be characterized in that extracted with a solvent selected from the group consisting of a mixed solvent thereof, but is not limited thereto.

In addition, the present invention provides a method for preventing or improving anti-oxidation, anti-inflammatory, pore-reducing, or wrinkles, comprising administering to a subject a composition containing a delphinium extract as an active ingredient.

In addition, the present invention provides a cosmetic method for antioxidation, anti-inflammatory, pore reduction, or prevention or improvement of wrinkles, which includes administering to a subject a composition containing a delphinium extract as an active ingredient.

In addition, the present invention provides antioxidant, anti-inflammatory, pore-reducing, or anti-wrinkle or improving uses of a composition containing a delphinium extract as an active ingredient.

In addition, the present invention provides a use of a composition containing the delphinium extract as an active ingredient for producing cosmetics used for antioxidation, anti-inflammatory, pore reduction, or prevention or improvement of wrinkles.

In addition, the present invention provides the use of the delphinium extract for producing a medicament for use in antioxidation or anti-inflammatory.

The delphinium extract according to the present invention has an antioxidant effect by inhibiting DPPH radicals, ABTS radicals, and TBARs and increasing PF, and exhibits anti-inflammatory effects by inhibiting hyaluronidase. In addition, the delphinium extract has an anti-wrinkle effect by inhibiting elastase or collagenase, and a pore-reducing effect by exhibiting an astringent effect. A pharmaceutical composition, food composition, or cosmetic composition having the above physiological activity It is hoped that it can be usefully used.

1 shows the phenol content according to the ethanol solvent concentration in the delphinium extract according to one embodiment of the present invention.
Figure 2a shows the results of confirming the DPPH radical scavenging activity of hot water and 70% ethanol extracts of delphinium according to an embodiment of the present invention.
Figure 2b shows the result of confirming the ABTS radical inhibitory activity of hot water and 70% ethanol extract of delphinium according to one embodiment of the present invention.
Figure 2c shows the results of measuring the PF of hot water and 70% ethanol extract of delphinium according to an embodiment of the present invention.
Figure 2d shows the results of confirming the TBARs inhibitory activity of hot water and 70% ethanol extract of delphinium according to an embodiment of the present invention.
Figure 3 shows the results of confirming the elastase inhibitory activity of hot water and 70% ethanol extract of delphinium according to one embodiment of the present invention.
Figure 4 shows the results of confirming the collagenase inhibitory activity of hot water and 70% ethanol extract of delphinium according to an embodiment of the present invention.
5 shows the result of confirming the astringent effect of hot water and 70% ethanol extract according to one embodiment of the present invention.
Figure 6 shows the results of confirming the hyaluronidase inhibitory activity of hot water and 70% ethanol extract of delphinium according to one embodiment of the present invention.

In one experimental example of the present invention, as a result of confirming the phenol content by distilled water and ethanol solvent concentration in the delphinium extract, it was confirmed that the phenol content was the highest in the extract extracted with 70% ethanol (see Experimental Example 1).

In another experimental example of the present invention, it was confirmed that the delphinium extract had an antioxidant effect by exhibiting DPPH radical scavenging activity, ABTS radical inhibitory activity, PF antioxidant activity, and TBARs inhibitory activity (see Experimental Example 2).

In another experimental example of the present invention, it was confirmed that the delphinium extract exhibited elastase inhibitory activity and collagenase inhibitory activity to have an anti-wrinkle effect (see Experimental Examples 3 and 4).

In another experimental example of the present invention, it was confirmed that the delphinium extract exhibited an astringent effect and had a pore-reducing effect (see Experimental Example 5).

In another experimental example of the present invention, it was confirmed that the delphinium extract exhibited hyaluronidase inhibitory activity and thus had an anti-inflammatory effect (see Experimental Example 6).

Therefore, the delphinium extract of the present invention exhibits DPPH radical scavenging activity, ABTS radical inhibitory activity, PF antioxidant activity, and TBARs inhibitory activity, elastase inhibitory activity, and collagenase inhibitory activity, astringent effect, and hyaluronida Since it exhibits inhibitory activity, it is expected that it can be used for antioxidant, anti-inflammatory, wrinkle improvement, and pore reduction purposes.

Hereinafter, the present invention will be described in detail.

The present invention is a delphinium ( Delphinium grandiflorum L. ) For antioxidant, containing an extract as an active ingredient; for anti-inflammatory use; for pore reduction; Or it provides a cosmetic composition for preventing and improving wrinkles.

In the present invention, " delphinium grandiflorum L. " is a perennial herbaceous wildflower that can be found in meadows or foothills of 2,900 m mountain range in Ranunculaceae genus Delphinium . Around July-August, flowers form a raceme at the end of the stem, and 3-15 flowers (bisexual) bloom, and because they contain delphinidin pigment, the flowers are purple to indigo blue. The delphinium extract may be a delphinium flower, petals, leaves, stems, fruits, rhizomes (rhizome), roots, or whole plant extracts including all of them, and according to an embodiment of the present invention, delphinium flower extract It can, but is not limited thereto. The swallowtail can be used without limitation, such as directly cultivated or commercially available.

In the present invention, "extract" refers to an extract obtained by the extraction treatment of the delphinium, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, It includes the extract itself and extracts of all formulations that can be formed using the extract. According to one embodiment of the present invention, the delphinium extract may be in the form of a dried product, but is not limited thereto.

In the present invention, the extraction method of the delphinium extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, reflux extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, solvent extraction, and the like, which are performed alone or in combination with two types. The above methods may be used in combination.

In the present invention, the type of extraction solvent used to extract the delphinium extract is not particularly limited, and according to a conventional method known in the art for extracting an extract from natural products, that is, conventional temperature and pressure It can be extracted using conventional solvents under the conditions of For example, the delphinium extract is water, C ₁ to C ₄ lower alcohol, n -hexane, ethyl acetate, acetone, acetonitrile, butyl acetate, 1,3-butylene glycol, methylene chloride, and mixtures thereof It may be extracted with one solvent selected from the group consisting of solvents, and according to an embodiment of the present invention, extraction may be performed using water or ethanol as a solvent, but is not limited thereto.

In the present invention, when the ethanol is used as a solvent to extract the delphinium, the concentration of ethanol is 10% to 100%, 10% to 90%, 10% to 80%, 20% to 100%, 20% to 90%, 20% to 80%, 30% to 100%, 30% to 90%, 30% to 80%, 40% to 100%, 40% to 90%, 40% to 80%, 50% to 100% , 50% to 90%, 50% to 80%, 60% to 100%, 60% to 90%, 60% to 80%, or 70%, but not limited thereto.

In the present invention, the prepared extract may then be filtered or concentrated or dried to remove the solvent, and both filtration, concentration and drying may be performed. For example, filtration may use filter paper or a vacuum filter, concentration may use a vacuum vacuum concentrator or vacuum rotary evaporator, and drying may be performed by vacuum drying, vacuum drying, boiling drying, spray drying, freeze drying, and the like. However, it is not limited thereto.

In the present invention, the number of extractions may be carried out one or more times, but as the extraction continues, the yield of the active ingredient significantly decreases, so it may not be economical to repeat the extraction five times or more. Accordingly, the number of extractions is preferably 1 to 5 times, and more preferably 2 to 5 repeated extractions, but is not limited thereto.

In the present invention, the delphinium extract may satisfy one or more of the following characteristics, but is not limited thereto:

(a) inhibit hyaluronidase;

(b) exhibits astringent action; and

(c) inhibition of elastase or collagenase.

In the present invention, "antioxidation" means inhibiting free radicals and reactive oxygen species that can cause oxidative damage to biomolecules, cross-link proteins, mutate DNA, and peroxidize lipids.

In the present invention, "preventing and improving wrinkles" means suppressing or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles already created.

The formulation of the cosmetic composition according to the present invention is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, mist, moisture cream, hand cream, hand lotion, foundation, It may be in the form of essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, cleansing oil, cleansing balm, body lotion, or body cleanser.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, and sphingolipids.

Any water-soluble vitamin can be used as long as it can be incorporated into cosmetics, but examples include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamin H. salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium salt of ascorbic acid-2-phosphate, etc.) included Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method, or a chemical synthesis method.

Any useful vitamin can be used as long as it can be formulated into cosmetics, but examples thereof include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol), and their derivatives (palmitic acid). Ascorbine, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) Included in the useful vitamins used in the invention. The useful vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial culture, enzymatic or chemical synthesis.

Any polymer peptide may be used as long as it can be incorporated into cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymer peptides can be purified and obtained by conventional methods such as a purification method from a culture medium of microorganisms, an enzyme method, or a chemical synthesis method, or can be used after being purified from natural products such as pig or cow dermis or silkworm silk fibers.

The polymeric polysaccharide may be any material as long as it can be incorporated into cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or salts thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

Any sphingolipid can be used as long as it can be incorporated into cosmetics, and examples thereof include ceramide, phytosphingosine, and sphingoglycolipid. Sphingolipids can be purified by conventional methods or obtained by chemical synthesis from mammals, fish, shellfish, yeast, or plants.

In the cosmetic composition of the present invention, in addition to the above essential ingredients, other ingredients normally formulated in cosmetics may be blended as necessary.

Other ingredients that may be added include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, A blood circulation accelerator, a cooling agent, an antiperspirant, purified water, etc. are mentioned.

Examples of the oil and fat component include ester oils, hydrocarbon oils, silicone oils, fluorine oils, animal fats and vegetable oils.

Examples of ester oils include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelislitol tetra2-ethylhexanoate , Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostea laurate Lil, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate Aryl, 2-ethyl stearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di(caprylic, capric acid) propylene glycol, propylene glycol dicaprylate, dicap Neopentyl glycol prinate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , Octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, dimalate Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, Cholesteryl Hydroxystearate, Cholesteryl Oleate, Dihydrocholesteryl Oleate, Physteryl Isostearate, Physteryl Oleate, Isocetyl 12-stealoylhydroxystearate, 12-stealoyylhydro and esters such as stearyl oxystearate and isostearyl 12-stealoylhydroxystearate.

Examples of hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and vaseline.

Examples of silicone oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealoxysiloxane copolymer, alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

Examples of fluorine-based fats and oils include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, algae oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil , cottonseed oil, palm oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, moonshine colostrum, marker damian nut oil, meadowsweet oil, egg yolk oil, beef tallow, horse oil, mink oil, orange raffia oil, jojoba oil , animal or plant fats and oils such as candelilla wax, carnaba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizer include water-soluble low-molecular moisturizers, fat-soluble molecular moisturizers, water-soluble polymers, and oil-soluble polymers.

Examples of water-soluble low-molecular moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactate, etc. are mentioned.

Cholesterol, cholesterol ester, etc. are mentioned as a fat-soluble low-molecular moisturizer.

Examples of water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, and the like. can

Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high molecular silicone.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE·POP (polyoxyethylene·polyoxypropylene) copolymer, POE·POP alkyl ether, polyether modified silicone, lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkyl amide sulfates, alkyl phosphates, POE alkyl phosphates, and alkyl amide phosphates. , alkyloylalkyl taurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinic acid salts, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. .

As the cationic surfactant, alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, chloride Benzalkonium, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salt, etc. are mentioned.

Amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. An amphoteric surfactant etc. are mentioned.

As organic and inorganic pigments, silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenolic resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, organic pigments such as silk powder, cellulose, CI pigment yellow and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts, such as zinc sodium cetylrate, zinc laurylrate, and calcium laurylrate; polyvalent metal salts of acyl amino acids such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; polyvalent metal salts of amide sulfonic acids such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hardened beef fatty acid acylarginine, etc. -acyl basic amino acids; N-acyl polypeptides such as N-lauroylglycylglycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, tetrafluoroethylene, and the like.

Examples of UV absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, and benzyl cinnamate. , paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl-diisopropylcinnamic acid ester mixture, uro Cannic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone , tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1 ,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Bactericides include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincophylthione, benzalkonium chloride, photosensitizer So No. 301, mononitroguacol sodium, undecyrenic acid, etc. are mentioned.

Examples of antioxidants include butylhydroxyanisole, propyl gallic acid, and elisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, and sodium monohydrogenphosphate.

Examples of alcohol include higher alcohols such as cetyl alcohol.

In addition, the blending components that may be added are not limited to these, and any of the above ingredients can be blended within a range not impairing the objects and effects of the present invention, but 0.01 to 5% by weight percent or 0.01 to 3% based on the total weight. Can be formulated in % weight percentage.

When the formulation of the present invention is a lotion, paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component can be used

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

As another aspect of the present invention, the present invention is a delphinium ( Delphinium grandiflorum L. ) For antioxidant, comprising an extract as an active ingredient; for anti-inflammatory use; for pore reduction; Or it provides a food composition for preventing and improving wrinkles.

In the present invention, "food" means a natural product or processed product containing one or more nutrients, preferably means a state that can be eaten directly through a certain degree of processing process, and usually means As, it means to include all health functional foods, beverages, food additives and beverage additives.

In the present invention, the food composition may be a health functional food composition, but is not limited thereto.

In the present invention, the "functional food" is the same term as food for special health use (FoSHU), medicine processed to efficiently display bioregulatory functions in addition to nutritional supply, It means a food with high medical effect, and can be manufactured into tablets, capsules, pills, granules, powders, liquids, flakes, pastes, syrups, gels, jellies, bars, or film formulations. Here, "functionality" means obtaining useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions.

When the delphinium extract of the present invention is used as a food additive, it can be added as it is or used together with other foods or food ingredients, and can be used appropriately according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the delphinium extract of the present invention may be added in an amount of 15% by weight or less, or 10% by weight or less based on the raw material. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

There is no particular limitation on the type of food. Examples of foods to which the above substances can be added include meat, sausages, bread, chocolates, candies, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice creams, various soups, beverages, tea, drinks, There are alcoholic beverages and vitamin complexes, and includes all health functional foods in a conventional sense.

The health beverage composition according to the present invention may contain various flavoring agents or natural carbohydrates as additional components, like conventional beverages. The aforementioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrins and cyclodextrins, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01-0.20 g, or about 0.04-0.10 g per 100 mL of the composition of the present invention.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, A carbonation agent used in carbonated beverages and the like may be contained. In addition, the composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not critical, but is generally selected in the range of 0.01-0.20 parts by weight per 100 parts by weight of the composition of the present invention.

The health functional food may have an advantage of having a more excellent effect by ingesting it in the form of inner beauty food. The inner beauty refers to food referred to as 'eating cosmetics or beauty food', which absorbs various ingredients good for the skin into the body and changes the skin constitution to a healthy one. You can select and consume the inner beauty food that suits your individual skin condition and lifestyle. For example, when cosmetics containing the cosmetic composition and inner beauty food containing the delphinium extract are mixed, the effect is significantly higher than using only cosmetics or drugs, resulting in more effective antioxidant, anti-inflammatory, pore reduction, and wrinkles. It may have the advantage of seeing a preventive or ameliorative effect.

In addition, the present invention provides a pharmaceutical composition for antioxidant or anti-inflammatory, containing an extract of Delphinium grandiflorum L. as an active ingredient.

In the present invention, “anti-inflammatory” refers to an action of suppressing or reducing inflammation, and may be used to include prevention, improvement, or therapeutic activity of inflammatory diseases. The inflammatory diseases include asthma, allergic and non-allergic rhinitis, gastritis, enteritis, ulcerative gastritis, nephritis, hepatitis, chronic obstructive pulmonary disease, pulmonary fibrosis, irritable bowel syndrome, inflammatory pain, migraine, headache, back pain, fibrosis myalgia, myofascial disease, viral infection (e.g. hepatitis C infection), bacterial infection, fungal infection, burn, surgical or dental injury, arthritis, rheumatoid arthritis, ankylosing spondylitis, Hodgkin's disease, pancreatitis, conjunctivitis, It may be one or more selected from the group consisting of iritis, scleritis, uveitis, dermatitis (including atopic dermatitis), eczema, and multiple sclerosis, but is not limited thereto.

The pharmaceutical composition according to the present invention may further include suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions. The excipient may be, for example, one or more selected from the group consisting of a diluent, a binder, a disintegrant, a lubricant, an adsorbent, a moisturizer, a film-coating material, and a controlled release additive.

The pharmaceutical compositions according to the present invention are powders, granules, sustained-release granules, enteric granules, solutions, eye drops, elsilic agents, emulsions, suspensions, spirits, troches, perfumes, and limonadese, respectively, according to conventional methods. , tablets, sustained-release tablets, enteric tablets, sublingual tablets, hard capsules, soft capsules, sustained-release capsules, enteric capsules, pills, tinctures, soft extracts, dry extracts, fluid extracts, injections, capsules, perfusate, It can be formulated and used in the form of an external agent such as a warning agent, lotion, pasta agent, spray, inhalant, patch, sterile injection solution, or aerosol, and the external agent is a cream, gel, patch, spray, ointment, warning agent , lotion, liniment, pasta, or cataplasma may have formulations such as the like.

Carriers, excipients and diluents that may be included in the pharmaceutical composition according to the present invention include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants.

Corn starch, potato starch, wheat starch, lactose, sucrose, glucose, fructose, di-mannitol, precipitated calcium carbonate, synthetic aluminum silicate, phosphoric acid as additives for tablets, powders, granules, capsules, pills, and troches according to the present invention Calcium monohydrogen, calcium sulfate, sodium chloride, sodium bicarbonate, purified lanolin, microcrystalline cellulose, dextrin, sodium alginate, methylcellulose, sodium carboxymethylcellulose, kaolin, urea, colloidal silica gel, hydroxypropyl starch, hydroxypropylmethyl excipients such as cellulose (HPMC), HPMC 1928, HPMC 2208, HPMC 2906, HPMC 2910, propylene glycol, casein, calcium lactate, and Primogel; Gelatin, gum arabic, ethanol, agar powder, cellulose phthalate acetate, carboxymethyl cellulose, calcium carboxymethyl cellulose, glucose, purified water, sodium caseinate, glycerin, stearic acid, sodium carboxymethyl cellulose, sodium methyl cellulose, methyl cellulose, microcrystalline cellulose, dextrin Binders such as hydroxycellulose, hydroxypropyl starch, hydroxymethylcellulose, purified shellac, starch arc, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinyl alcohol, and polyvinylpyrrolidone may be used, Hydroxypropyl Methyl Cellulose, Corn Starch, Agar Powder, Methyl Cellulose, Bentonite, Hydroxypropyl Starch, Sodium Carboxymethyl Cellulose, Sodium Alginate, Calcium Carboxymethyl Cellulose, Calcium Citrate, Sodium Lauryl Sulfate, Silicic Anhydride, 1-Hydroxy Propyl cellulose, dextran, ion exchange resin, polyvinyl acetate, formaldehyde-treated casein and gelatin, alginic acid, amylose, guar gum, sodium bicarbonate, polyvinylpyrrolidone, calcium phosphate, gelled starch, gum arabic, disintegrants such as amylopectin, pectin, sodium polyphosphate, ethyl cellulose, white sugar, magnesium aluminum silicate, di-sorbitol solution, and light anhydrous silicic acid; Calcium stearate, magnesium stearate, stearic acid, hydrogenated vegetable oil, talc, lycopod, kaolin, petrolatum, sodium stearate, cacao butter, sodium salicylate, magnesium salicylate, polyethylene glycol (PEG) 4000, PEG 6000, liquid paraffin, hydrogen Added soybean oil (Lubri wax), aluminum stearate, zinc stearate, sodium lauryl sulfate, magnesium oxide, macrogol, synthetic aluminum silicate, silicic anhydride, higher fatty acid, higher alcohol, silicone oil, paraffin oil, polyethylene glycol fatty acid ether, Lubricants such as starch, sodium chloride, sodium acetate, sodium oleate, dl-leucine, and light anhydrous silicic acid may be used.

Additives for the liquid formulation according to the present invention include water, dilute hydrochloric acid, dilute sulfuric acid, sodium citrate, sucrose monostearate, polyoxyethylene sorbitol fatty acid esters (tween esters), polyoxyethylene monoalkyl ethers, lanolin ethers, Lanolin esters, acetic acid, hydrochloric acid, aqueous ammonia, ammonium carbonate, potassium hydroxide, sodium hydroxide, prolamine, polyvinylpyrrolidone, ethyl cellulose, sodium carboxymethyl cellulose, and the like may be used.

In the syrup according to the present invention, a solution of white sugar, other sugars, or a sweetener may be used, and aromatics, coloring agents, preservatives, stabilizers, suspending agents, emulsifiers, thickeners, etc. may be used as necessary.

Purified water may be used in the emulsion according to the present invention, and emulsifiers, preservatives, stabilizers, fragrances, etc. may be used as needed.

Suspension agents according to the present invention include acacia, tragacantha, methylcellulose, carboxymethylcellulose, sodium carboxymethylcellulose, microcrystalline cellulose, sodium alginate, hydroxypropylmethylcellulose (HPMC), HPMC 1828, HPMC 2906, HPMC 2910, etc. Agents may be used, and surfactants, preservatives, stabilizers, colorants, and fragrances may be used as needed.

Injections according to the present invention include distilled water for injection, 0.9% sodium chloride injection, IV injection, dextrose injection, dextrose + sodium chloride injection, PEG, lactated IV injection, ethanol, propylene glycol, non-volatile oil-sesame oil , solvents such as cottonseed oil, peanut oil, soybean oil, corn oil, ethyl oleate, isopropyl myristate, and benzene benzoate; solubilizing agents such as sodium benzoate, sodium salicylate, sodium acetate, urea, urethane, monoethylacetamide, butazolidine, propylene glycol, twins, nijuntinamide, hexamine, and dimethylacetamide; buffers such as weak acids and their salts (acetic acid and sodium acetate), weak bases and their salts (ammonia and ammonium acetate), organic compounds, proteins, albumin, peptone, and gums; tonicity agents such as sodium chloride; Stabilizers such as sodium bisulfite (NaHSO ₃ ) carbon dioxide gas, sodium metabisulfite (Na ₂ S ₂ O ₅ ), sodium sulfite (Na ₂ SO ₃ ), nitrogen gas (N ₂ ), ethylenediaminetetraacetic acid; Sulfating agents such as sodium bisulfide 0.1%, sodium formaldehyde sulfoxylate, thiourea, ethylenediamine disodium tetraacetate, acetone sodium bisulfite; analgesics such as benzyl alcohol, chlorobutanol, procaine hydrochloride, glucose, and calcium gluconate; Suspending agents such as Siemesis sodium, sodium alginate, Tween 80, aluminum monostearate may be included.

The suppository according to the present invention includes cacao butter, lanolin, witapsol, polyethylene glycol, glycerogelatin, methylcellulose, carboxymethylcellulose, a mixture of stearic acid and oleic acid, subanal, cottonseed oil, peanut oil, palm oil, cacao butter + Cholesterol, Lecithin, Lannet Wax, Glycerol Monostearate, Tween or Span, Imhausen, Monolen (Propylene Glycol Monostearate), Glycerin, Adeps Solidus, Buytyrum Tego-G -G), Cebes Pharma 16, Hexalide Base 95, Cotomar, Hydroxycote SP, S-70-XXA, S-70-XX75 (S-70-XX95), Hyde Hydrokote 25, Hydrokote 711, Idropostal, Massa estrarium (A, AS, B, C, D, E, I, T), Massa-MF, Masupol, Masupol-15, Neosupostal-N, Paramound-B, Suposiro (OSI, OSIX, A, B, C, D, H, L), suppository type IV (AB, B, A, BC, BBG, E, BGF, C, D, 299), Supostal (N, Es), Wecobi (W, R, S, M, Fs), testosterone triglyceride base (TG-95, MA, 57) and The same mechanism can be used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, for example, starch, calcium carbonate, sucrose, etc. ) or by mixing lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used.

Liquid preparations for oral administration include suspensions, solutions for oral administration, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. there is. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is the type of patient's disease, severity, activity of the drug, It may be determined according to factors including sensitivity to the drug, administration time, route of administration and excretion rate, duration of treatment, drugs used concurrently, and other factors well known in the medical field.

The pharmaceutical composition according to the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered single or multiple times. Considering all of the above factors, it is important to administer an amount that can obtain the maximum effect with the minimum amount without side effects, which can be easily determined by a person skilled in the art to which the present invention belongs.

The pharmaceutical composition of the present invention can be administered to a subject by various routes. All modes of administration can be envisaged, eg oral administration, subcutaneous injection, intraperitoneal administration, intravenous injection, intramuscular injection, paraspinal space (intrathecal) injection, sublingual administration, buccal administration, intrarectal insertion, vaginal It can be administered by intraoral insertion, ocular administration, otic administration, nasal administration, inhalation, spraying through the mouth or nose, dermal administration, transdermal administration, and the like.

The pharmaceutical composition of the present invention is determined according to the type of drug as an active ingredient together with various related factors such as the disease to be treated, the route of administration, the age, sex, weight and severity of the disease of the patient.

In addition, the present invention provides a quasi-drug composition for anti-oxidation or anti-inflammatory, containing Delphinium grandiflorum L. extract as an active ingredient.

In the present invention, "quasi-drugs" refers to items that have a milder effect than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, mitigating, treating or preventing human or animal diseases, for example, according to the Pharmaceutical Affairs Act of the Republic of Korea. According to the definition, quasi-drugs are items excluding items used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans and animals, and products with minor or no direct action on the human body.

In addition, the quasi-drugs may include external skin preparations and personal hygiene products. For example, it may be a disinfectant cleanser, shower foam, gargreen, wet tissue, detergent soap, hand wash, body cleanser, soap, mask, cream, lotion, essence, spray, or ointment, but is not limited thereto.

When the quasi-drug composition according to the present invention is used as a quasi-drug additive, the composition may be added as it is or used together with other quasi-drugs or quasi-drug ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use.

The quasi-drug composition of the present invention may be prepared in the form of, for example, a general emulsified formulation and a solubilized formulation. For example, it may have formulations such as emulsions, creams, ointments, sprays, oil gels, gels, oils, aerosols, and smokers such as lotions, but it may be used without limitation as long as it exhibits the pest control inducing effect of the present invention. . In addition, the quasi-drug composition is appropriately mixed as necessary with oil, water, surfactant, moisturizer, lower alcohol having 1 to 4 carbon atoms, thickener, chelating agent, colorant, preservative or flavoring agent, which are generally formulated in quasi-drug compositions, in each dosage form and can be used.

As another aspect of the present invention, the present invention provides an antioxidative, anti-inflammatory, pore-reducing, or A method for preventing or improving wrinkles is provided.

As another aspect of the present invention, the present invention provides a cosmetic method for antioxidation, anti-inflammatory, pore reduction, or prevention or improvement of wrinkles, comprising administering to a subject a composition containing an extract of a delphinium extract as an active ingredient. .

As another aspect of the present invention, the present invention provides antioxidant, anti-inflammatory, pore reduction, or wrinkle prevention or improvement uses of a composition containing a delphinium extract as an active ingredient.

As another aspect of the present invention, the present invention provides a use of a composition comprising a delphinium extract as an active ingredient for producing a cosmetic product used for antioxidation, anti-inflammatory, pore reduction, or prevention or improvement of wrinkles.

As another aspect of the present invention, the present invention provides the use of a delphinium extract for producing a medicament used for antioxidant or anti-inflammatory purposes.

In the present invention, "prevention" means any action that suppresses or delays the onset of a desired disease, and "treatment" means that a desired disease and its metabolic abnormalities are prevented by administration of the pharmaceutical composition according to the present invention. It means any action that is ameliorated or changed beneficially, and "improvement" means any action that reduces a parameter related to a target disease, for example, the severity of a symptom, by administration of the composition according to the present invention.

In the present invention, "individual" means a subject in need of treatment of a disease, and more specifically, a human or non-human primate, mouse, rat, dog, cat, horse, and It means mammals such as cattle.

In the context of the present invention, "administration" means providing a given composition of the present invention to a subject by any suitable method.

Hereinafter, preferred embodiments and experimental examples are presented to aid understanding of the present invention. However, the following examples and experimental examples are only provided to more easily understand the present invention, and the contents of the present invention are not limited by the following examples.

[Example]

Example 1. Delphinium sample preparation

Delphinium grandiflorum L. was purchased from Donga Flower Materials (Busan, South Korea), separated from stems and flowers, dried in a 50℃ dry oven (FO600M, Jeiotech, Daejeon, Korea), and dried flowers It was pulverized with 40 mesh and powdered to be used as a sample.

Example 2. Preparation of delphinium extract

For the experiment, distillation water (D.W) and ethanol were used as extraction solvents. For hot water extract, 0.5 g of delphinium flower powder was immersed in 100 mL of distilled water, heated until the extract volume reached 50 mL, cooled, and shaken. It was placed in a shaking incubator (24 hr/200 rpm) and stirred and extracted. In the case of ethanol extract, 0.5 g of delphinium flower powder was added to 50 mL of ethanol prepared at different concentrations (10 μg/mg to 100 μg/mg), followed by stirring and extraction in a shaking incubator (24 hr/200 rpm). Each extract was prepared using Whatman No. After filtering with 1 filter paper (Whatman inc., piscataway, New jersey, USA), the extract was used as an extract sample after concentration by concentration as necessary.

Example 3. Quantification of total phenolic compounds (TPC)

TPC content was measured according to the Folin-Denis method (Journal of Biological Chemistry, 12(2), 239-243.). 1 mL of 100% ethanol and 5 mL of distilled water were added to 1 mL of the delphinium sample extract, 0.5 mL of 1 N Folin-ciocalteu reagent was added, vortexing was performed, and the mixture was allowed to stand for 5 minutes. Then, after adding 1 mL of 5% Na ₂ CO ₃ and reacting in the dark for 1 hour, absorbance was measured using a UV-vis photospectrometer (Optizen 3220 UV, Merasys Co. Ltd., Seoul, Korea) at a wavelength of 725 nm. did The total phenol content was quantified by converting the amount in comparison with a standard curve obtained by measuring gallic acid prepared by concentration.

Example 4. Measurement of DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity

DPPH radical scavenging activity was measured according to the method of Blios (Bios MS (1958), Nature 181: 11 99-1200). In the reaction zone, 3 mL of 100 μM DPPH was added to each 1.0 mL of hot water and ethanol sample extract prepared by concentration (25 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL), and in the control group, distilled water 1 was added instead of the sample. mL was added and vortexed. Thereafter, after dark reaction at room temperature for 15 minutes, absorbance was measured at a wavelength of 517 nm using a UV-vis photospectrometer (Optizen 3220 UV, Merasys Co. Ltd., Seoul, Korea). As the positive control, ascorbic acid was used. On the other hand, DPPH radical scavenging activity (%) was calculated by Equation 1 below.

[Equation 1]

DPPH radical scavenging activity (%) = (1-absorbance of reactive group / absorbance of control group) × 100.

Example 5. Measurement of ABTS (2,2'-azino-bis (3-ethylbenzothiazoline 6-sulphonic acid) radical inhibitory activity

ABTS radical inhibitory activity was measured according to the method of Pellegrini et al. (Pellegrini N, Re R, Yang M, Rice-Evans C (1998), Methods Enzymology 299: 379-389). Specifically, after mixing 50 mL of 7 mM ABTS and 0.88 mL of 140 mM K ₂ S ₂ O ₈ and reacting in the dark for about 15 hours or more to form radicals, they were mixed with 100% ethanol in a ratio of about 1:88 and at 734 nm An ABTS solution adjusted so that the absorbance value of the control was about 0.7±0.02 was used. In the reaction group, 0.2 mL of the delphinium extract sample and 4 mL of the ABTS solution were mixed, and in the control group, 200 μL of distilled water was added instead of the sample, vortexed, reacted for 2 minutes, and absorbance was measured at a wavelength of 734 nm. Ascorbic acid was used as a positive control, and ABTS radical inhibitory activity (%) was calculated by the following formula 2.

[Equation 2]

ABTS radical inhibitory activity (%) = (1-absorbance of reactive group/absorbance of control group) × 100.

Example 6. PF (Antioxidant protection factor) measurement

Antioxidant protection factor (PF) was measured according to the method of Andarwulan and Shetty (Andarwulan N, Shetty K (1999). J Agric Food Chem, 47: 1776-1780). Specifically, in order to prepare a beta-carotene (β-carotene) solution, 0.05 g of beta-carotene and 50 ml of chloroform were mixed, placed in a flask, and then wrapped with silver foil to concentrate. Then, 0.02 mL of linoleic acid, 0.184 mL of Tween 40, and 50 mL of H ₂ O ₂ were added to prepare a beta-carotene emulsion. Thereafter, 5 mL of the emulsion was added to 0.1 mL of the delphinium extract sample, vortexed, reacted in a water bath set at 50 ° C. for 30 minutes, blocked from light, cooled in ice water for 5 minutes, and absorbance was measured at a wavelength of 470 nm. BHT was used as a positive control group, and the PF value was calculated by Equation 3 below.

[Equation 3]

PF = absorbance of reaction/absorbance of control.

Example 7. Measurement of Thiobarbituric acid reactive substances (TBARs) inhibitory activity

TBARs activity inhibition was measured according to the method of Buege and Aust (Buege JA, Aust SD (1978). Methods Enzymol 52: 302-310). Specifically, 100 mL each of 5% linoleic acid and 1% tween 40 was added and mixed with a hand mixer to prepare an emulsion. Then, 0.8 mL of the emulsion was added to 0.2 mL of the delphinium extract sample and reacted in a water bath at 50 °C for 16 hours or more. A stock solution was prepared by adding 4 mL of 15% trichloroacetic acid (TCA), 0.375% thiobarbituric acid (TBA), and 4 mL of 0.25 M HCl, and then adding 4 mL of TBA reagent prepared by mixing 2% BHT. Thereafter, they were mixed well and heated in a water bath at 100° C. for 15 minutes, then it was confirmed that the color of the control group had changed to pink, and then cooled in ice water for 10 minutes. Then, after centrifugation at 2,000 rpm for 20 minutes in a centrifuge, precipitate was allowed to stand for 10 minutes, and absorbance was measured at a wavelength of 532 nm. BHT was used as a positive control, and the TBARs activity inhibitory activity (%) was calculated by Equation 4 below.

[Equation 4]

TBARs inhibitory activity (%) = (1-absorbance of reactive group / absorbance of control group) × 100.

Example 8. Measurement of elastase inhibitory effect

Elastase inhibitory effect was measured according to the method of Kraunsoe et al. (Kraunsoe JA, Claridge TD, Lowe G (1996). Biochemistry 35: 9090-9096). Specifically, substrate 0.8 mM N-succinyl-(Ala)3-p-nitroanilide (Sigma Aldrich Co., St Louis, MO, USA) dissolved in 0.2 M Tris-HCl buffer (pH 8.0) in 0.1 mL of delphinium extract sample. 0.1 mL was added, and 1 mL of buffer solution (pH 8.0) was added. Then, 0.1 mL of 0.3125 U/mL pig pancreatic elastase (Porcine pancreatic elastase, Sigma Aldrich Co.) dissolved in buffer was added, vortexed, and reacted in a water bath at 37° C. for 20 minutes. After taking it out of the water bath and cooling it in ice water for 5 minutes, the absorbance was measured at a wavelength of 410 nm. EGCG ((-)-epigallocatechin-3-o-gallate) was used as a positive control, and the elastase inhibitory activity (%) was calculated by the following formula 5.

[Equation 5]

Elastase inhibitory activity (%) = (1-absorbance of reactive group / absorbance of control group) × 100.

Example 9. Collagenase inhibitory effect measurement

The collagenase inhibitory effect was measured according to the method of Wunsch-Heindrich (Wunsch E, Heidrich HG (1963). Hoppe-Seyler's Z Physiol Chem 333: 149-151). 0.25 mL of 0.4 mM 4-phenylazobenzyloxycarbonyl-pro-Leu-Gly-Pro-D-Arg dissolved in a buffer solution (pH 7.5) prepared by adding 4 mM CaCl2 to 0.1 M tris-HCl to 0.1 mL of the delphinium extract sample. put in Then, 0.15 mL of buffer (pH 7.5) was added. After that, 0.15 mL of 100 U/mL collagenase from clostridium histolyticum diluted in buffer was added. Then, after reacting in a water bath at 25 ° C. for 20 minutes, 0.5 mL of 6% citric acid as a termination reagent was added, and 2 mL of ethyl acetate was added and vortexed, followed by vortexing at a wavelength of 320 nm. The absorbance was measured at. EGCG was used as a positive control, and collagenase inhibitory activity (%) was calculated by the following formula 6.

[Equation 6]

Collagenase inhibitory activity (%) = (1-absorbance of reaction group / absorbance of control group) × 100.

Example 10. Convergence Effect Activity Measurement

Astringent effect was measured according to the method of Lee JT, Jeong YS, and An BJ (The Korea Journal of Herbology, 17(2), 51-51). Specifically, hemoglobin, a blood protein similar to skin protein, was used as a substrate. After putting 0.7 mL of delphinium extract sample and 0.35 mL of 0.4% hemoglobin solution (Sigma Aldrich Co., St Louis, MO, USA) dissolved in 0.2 M sodium phosphate buffer (pH 7.5) into the e-Tube, 0.35 mL of buffer (pH 7.5) was added. After vortexing, centrifugation was performed at 13,500 rpm for 5 minutes using a centrifuge (FO600M, Jeiotech, Daejeon, Korea) to settle coagulated blood proteins and absorbance was measured at a wavelength of 576 nm. Tannic acid was used as a positive control, and the astringent effect (%) was calculated by Equation 7 below.

[Equation 7]

Convergence effect (%) = (1-absorbance of reaction group/absorbance of control group)×100.

Example 11. Measurement of hyaluronidase inhibitory effect

Hyaluronidase (HAase) inhibitory effect was measured according to Dorfman and Ott (Dorfman A, Ott ML (1948), J Biol Chem 172: 367-375). Specifically, 0.25 mL of delphinium extract sample by concentration (50, 100, 200, 400 μg/mL phenolics concentration) and 0.25 mL of HAase (1,000 U/mL) dissolved in 20 mM sodium phosphate buffer (pH 6.9) After mixing mL, it was reacted for 5 minutes in a 38 ° C water bath. Then, 0.25 mL of the substrate hyaluronic acid (4 mg/mL) dissolved in 0.3 M phosphate buffer (pH 5.3) was added, and reacted again at 38° C. for 45 minutes. Thereafter, 2.5 mL of an albumin solution dissolved in 0.04 M acetate buffer (pH 3.7) as a termination reagent was added. Then, it was allowed to stand for 5 minutes and transmittance (%) was measured at 600 nm. As a control, 0.25 mL of distilled water was added instead of the sample and reacted. Hyaluronidase inhibitory activity (%) was calculated by Equation 9 below.

[Equation 9]

Hyaluronidase inhibitory activity (%) = (1-permeability of reaction group / transmittance of control group) × 100.

Example 12. Statistical analysis

The experimental results of this study were expressed as mean and standard error. In addition, to verify the significance of the average value of the results using SPSS software 25 (SPSS Inc., Chicago, IL, USA), one-way ANOVA was performed during the average analysis, and post-hoc was performed using Duncan's method. The test was performed and mutual comparisons were made at the p<0.05 level.

[Experimental Example]

Experimental Example 1. Confirmation of phenol (TPC) content of delphinium extract

In this study, in order to quantify total phenolic compounds (TPC) prior to conducting antioxidant experiments of delphinium flower extracts, TPC of extracts using distilled water and ethanol by concentration (10% to 100% ethanol) as a solvent compared. The results are shown in Figure 1.

As shown in FIG. 1, the phenol content per 1 g of the 70% ethanol extract was the highest at 13.38 mg, and the hot water extract was the second highest at 13.37 mg per 1 g. According to the above results, distilled water and 70% ethanol were used as solvents in subsequent experiments.

Experimental Example 2. Confirmation of antioxidant effect of delphinium extract

For antioxidant experiments using the delphinium extract, the extract was tested at four phenolic concentrations of 25, 50, 75 and 100 μg/mL.

2-1. Confirmation of DPPH radical scavenging activity

The DPPH radical is used as a measure of the antioxidant effect according to the degree of discoloration from dark purple by reduction by aromatic amines due to the electron donating ability of antioxidants. Accordingly, the DPPH radical scavenging activity of the delphinium flower extract was confirmed. The results are shown in Figure 2a.

As shown in FIG. 2a, in the case of the hot water extract, the scavenging activity was 86.04, 87.92, 87.92, and 90.07% at TPC concentrations of 25, 50, 75, and 100 μg/mL, respectively, and the 70% ethanol extract showed 93.38, 93.38, and 90.07%, respectively. Clearing activities of 98.46, 99.81 and 100.00% were exhibited.

This means that the DPPH radical scavenging activity significantly increased in proportion to the TPC concentration of the delphinium flower extract. In addition, BHT used as a positive control was 88.69, 94.90, 95.59, and 95.39% at the same concentration, which means that the delphinium extract has excellent antioxidant activity against DPPH radicals.

2-2. Confirmation of ABTS radical inhibition activity

In the measurement of ABTS radical inhibitory activity, the ABTS radical generated by the reaction with potassium persulfate is removed by the antioxidant properties of the phenolic compound contained in the extract, and the blue-green color characteristic of the radical is pale due to the radical inhibitory activity of the phenolic compound. It is a method for measuring antioxidant activity using green discoloration. Accordingly, the ABTS radical inhibitory activity of the delphinium flower extract was confirmed. The results are shown in Figure 2b.

As shown in FIG. 2b, at TPC concentrations of 25, 50, 75, and 100 μg/mL, the hot water extract exhibited antioxidant activity of 39.44, 74.20, 94.34, and 99.54%, respectively, and the 70% ethanol extract showed antioxidant activity of 41.16, 67.88, and 95.38, respectively. , and showed antioxidant activity of 100.20%. On the other hand, ascorbic acid used as a positive control also showed antioxidant activities of 29.23, 67.43, 99.33, and 98.85% when the experiment was conducted at the same concentration. This means that the antioxidant activity of the delphinium flower extract against the ABTS radical is excellent.

2-3. Confirmation of PF antioxidant activity

PF is an antioxidant effect measurement method using beta-carotene linoleate system. Free radicals generated in the process of rancidity of unsaturated fatty acids oxidize beta-carotene to decolorize beta-carotene. . The antioxidant effect of the delphinium flower extract was confirmed by measuring PF using this principle. The results are shown in Figure 2c.

As shown in FIG. 2c, the PF activity was 1.17, 1.22, 1.25, and 1.26 at 25, 50, 75, and 100 μg/mL TPC concentrations of the hot water extract, and 1.13 at the same concentration of the 70% ethanol extract, respectively. , PF activity of 1.17, 1.22, and 1.27. BHT, a positive control, showed PF activities of 1.10, 1.07, 1.24, 1.25, and 1.27 at the same concentrations. Both the hot water extract and the 70% ethanol extract showed a significant increase in PF activity in a concentration-dependent manner.

2-4. Confirmation of TBARs inhibitory activity

The TBARs inhibitory activity of the delphinium flower extract was confirmed. The results are shown in Figure 2d.

As shown in FIG. 2d, the hot water extract showed antioxidant activities of 10.65, 18.24, 22.31, and 24.05% at 25, 50, 75, and 100 μg/mL TPC concentrations, respectively, and in the case of 70% ethanol extract, respectively, 11.81, 29.61, and 38.07 , and showed antioxidant activity of 69.55%. It was confirmed that the antioxidant activity of all extracts increased in a concentration-dependent manner, and it was confirmed that the 70% ethanol extract had a better TBARs effect than the hot water extract.

From the above results, it can be seen that the delphinium flower extract exhibits high activity against water-soluble and fat-soluble antioxidants and contains a large amount of water-soluble and fat-soluble antioxidant components.

Experimental Example 3. Confirmation of elastase inhibitory activity of delphinium extract

Four phenols at 50, 100, 200 and 400 μg/mL inhibit elastase, an enzyme that reduces skin elasticity and creates wrinkles by decomposing elastin, which maintains skin elasticity in the human body. Concentrations of the delphinium hot water extract and 70% ethanol extract were confirmed after treatment. The results are shown in Figure 3.

As shown in Figure 3, the elastase inhibitory effect was not shown in the hot water extract, and the inhibitory activity of 7.52, 22.46, 26.91, and 41.21%, respectively, at TPC concentrations of 50, 100, 200, and 400 μg/mL of the 70% ethanol extract. EGCG, a positive control group, exhibited 30.52, 48.52, 76.00, and 100.00% inhibitory activity at TPC concentrations of 50, 100, 200, and 400 μg/mL. Therefore, it was confirmed that the 70% ethanol extract of the delphinium flower had an excellent elastase inhibitory effect.

Experimental Example 4. Confirmation of Collagenase Inhibitory Activity of Delphinium Extract

Collagen is a connective tissue constituting the dermis layer of the skin together with elastin. The activity of collagenase increases due to ultraviolet light or natural aging, which is the cause of photoaging, and collagen is also decomposed accordingly, which can cause wrinkles. In order to confirm whether the delphinium flower extract inhibits this mechanism, the collagenase inhibitory activity was confirmed. The results are shown in FIG. 4 .

As shown in FIG. 4, the hot water extract did not show collagenase inhibitory effect, and the inhibitory activity of 15.84, 29.57, 68.64, and 82.58% at TPC concentrations of 50, 100, 200, and 400 μg/mL of the 70% ethanol extract, respectively. It was confirmed that it increased in a concentration dependent manner. On the other hand, EGCG, a positive control, showed inhibitory activities of 0.00, 19.56, 30.66, and 41.06% at the same TPC concentrations of 25, 50, 75, and 100 μg/mL. That is, the 70% ethanol extract of delphinium flower exhibited an inhibitory activity nearly twice that of EGCG. This means that the collagenase inhibitory activity of the 70% ethanol extract of the delphinium flower is very good.

From the above results, it was found that the delphinium extract of the present invention exhibits elastase and collagenase inhibitory activity, thereby exhibiting a skin wrinkle improvement effect.

Experimental Example 5. Confirmation of astringent effect of delphinium extract

Astringent basically has the meaning of convergence that wrinkles shrink, and Astringent activity is known to have the effect of reducing the permeability of the cell membrane by making the surface tissue dense. Accordingly, the astringent activity was measured to confirm the astringent effect of the delphinium flower extract. The results are shown in FIG. 5 .

As shown in FIG. 5, the hot water extract did not show any effect, but in the case of the 70% ethanol extract, the activity was 40.93 and 44.82% at 200 and 400 μg/mL TPC concentrations, respectively. In the case of tannic acid, it was confirmed that the activity was 25.19 and 96.69% at 200 and 400 μg/mL TPC concentrations, respectively. In addition, at a TPC concentration of 200 μg/mL or less, the 70% ethanol extract of the swallowtail flower exhibited a better astringent effect than the control tannic acid, indicating that the pore reduction effect was excellent even at a low concentration. Therefore, it was confirmed that the astringent effect of the 70% ethanol extract of the delphinium flower was excellent.

From the above results, it can be seen that the delphinium extract has an excellent astringent effect and exhibits a pore-reducing effect.

Experimental Example 6. Confirmation of hyaluronidase inhibitory activity of delphinium extract

Hyaluronic acid is a high-molecular polysaccharide in which glucuronic acid and glucosamine are continuously linked. Hyaluronic acid in the form of a polymer suppresses inflammation by inhibiting the phagocytic activity of macrophages, and small molecules form inflammation during wound healing. Therefore, in order to confirm the anti-inflammatory effect of the delphinium extract, the inhibitory activity of hyaluronidase, an enzyme related to inflammation, was tested at four phenol concentrations of 50, 100, 200 and 400 μg/mL. It was confirmed after treatment with hot water extract and 70% ethanol extract. The results are shown in FIG. 6 .

As shown in FIG. 6, the hot water extract exhibited inhibitory activities of 21.48, 27.33, 31.06, and 24.52% at TPC concentrations of 50, 100, 200, and 400 μg/mL, respectively, and the 70% ethanol extract exhibited inhibitory activities of 11.71, 12.68, and 12.68, respectively. It showed inhibitory activity of 11.92 and 16.81%. PDTC, a positive control, showed inhibitory activities of 6.18, 14.92, 24.69, and 48.09% at TPC concentrations of 50, 100, 200, and 400 μg/mL. The hot water extract showed the highest inhibitory effect at a TPC concentration of 200 μg/mL, and showed a superior effect than the 70% ethanol extract. The above results indicate that both the delphinium flower hot-water extract and the 70% ethanol extract have an excellent effect of inhibiting the activity of hyaluronidase, a major enzyme involved in the inflammatory response.

In addition, it is known that high-molecular hyaluronic acid has a moisturizing effect of increasing and maintaining the moisture content of the skin. Through the hyaluronidase inhibitory activity of the delphinium extract, it can be used as a cosmetic material having anti-inflammatory and moisturizing effects. Applicability was confirmed.

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (14)

Containing delphinium grandiflorum L. extract as an active ingredient, for antioxidant; for anti-inflammatory use; for pore reduction; Or a cosmetic composition for preventing and improving wrinkles.
According to claim 1,
The cosmetic composition, characterized in that the delphinium extract is a delphinium flower extract.
According to claim 1,
The delphinium extract inhibits DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical inhibition, and TBARs (Thiobarbituric acid reactive substances) inhibition , or PF (Antioxidant protection factor) characterized in that it has an increasing activity, a cosmetic composition.
According to claim 1,
The cosmetic composition, characterized in that the delphinium extract satisfies at least one of the following characteristics:
(a) inhibit hyaluronidase;
(b) exhibits astringent action; and
(c) inhibition of elastase or collagenase.
According to claim 1,
The delphinium extract is a group consisting of water, C ₁ to C ₄ lower alcohol, n-hexane, ethyl acetate, acetone, acetonitrile, butyl acetate, 1,3-butylene glycol, methylene chloride, and mixed solvents thereof Characterized in that extracted with a solvent selected from, a cosmetic composition.
Containing delphinium grandiflorum L. extract as an active ingredient, for antioxidant; for anti-inflammatory use; for pore reduction; Or a food composition for preventing and improving wrinkles.
According to claim 6,
The food composition, characterized in that the delphinium extract is a delphinium flower extract.
According to claim 6,
The delphinium extract inhibits DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical inhibition, and TBARs (Thiobarbituric acid reactive substances) inhibition , or PF (Antioxidant protection factor) characterized in that it has an increasing activity, food composition.
According to claim 6,
The food composition, characterized in that the delphinium extract satisfies one or more of the following characteristics:
(a) inhibit hyaluronidase;
(b) exhibits astringent action; and
(c) inhibition of elastase or collagenase.
According to claim 6,
The delphinium extract is a group consisting of water, C ₁ to C ₄ lower alcohol, n-hexane, ethyl acetate, acetone, acetonitrile, butyl acetate, 1,3-butylene glycol, methylene chloride, and mixed solvents thereof Characterized in that extracted with a solvent selected from, a food composition.
A swallowtail ( Delphinium grandiflorum L. ) Containing an extract as an active ingredient, for antioxidant, or anti-inflammatory pharmaceutical composition.
According to claim 11,
The delphinium extract inhibits DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical inhibition, and TBARs (Thiobarbituric acid reactive substances) inhibition , Or PF (Antioxidant protection factor) characterized in that it has an increasing activity, a pharmaceutical composition.
According to claim 11,
The pharmaceutical composition, characterized in that the delphinium extract inhibits hyaluronidase (hyaluronidase).
Delphinium grandiflorum L. Containing an extract as an active ingredient, anti-oxidation or anti-inflammatory quasi-drug composition.

Images