KR20230077349A - Cosmetic composition for improving skin wrinkle comprising mixed extracts of arctium lappa seed, morinda citrifolia, euphoria longana and saururus chinensis as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition having skin wrinkle improvement and elasticity enhancing effects, containing mixed extracts of Woobangja, Noni, Longan, and Sambaekcho as active ingredients, and more particularly, the mixed extract according to the present invention contains each component alone. It relates to a cosmetic composition having an excellent effect by improving skin elasticity and improving wrinkles compared to the case where the present invention is used.

Description

COSMETIC COMPOSITION FOR IMPROVING SKIN WRINKLE COMPRISING MIXED EXTRACTS OF ARCTIUM LAPPA SEED, MORINDA CITRIFOLIA, EUPHORIA LONGANA AND SAURURUS CHINENSIS AS ACTIVE INGREDIENT}

The present invention relates to a cosmetic composition having an effect of improving skin wrinkles and enhancing skin elasticity, containing a mixed extract of Woobangja, Noni, Longan, and Sambaekcho as an active ingredient. More specifically, the cosmetic composition according to the present invention has excellent skin elasticity enhancement and wrinkle improvement effects compared to the case of using each component alone, and can be usefully used as a cosmetic composition that is safe and has excellent effects on the skin.

As interest in skin beauty as well as health increases worldwide, interest in aging is increasing in order to live a healthy old age. Skin aging is classified into intrinsic aging and extrinsic aging. Endogenous aging refers to natural aging that progresses over time, and extrinsic aging refers to aging that is progressed by environmental factors such as ultraviolet rays, cigarette smoke, and fine dust. In particular, exposure to ultraviolet rays is a major cause of extrinsic aging and is also called photoaging. Photoaging accounts for 80% of skin aging, and photoaged skin is characterized by wrinkles, loss of elasticity, dryness, rough appearance and pigmentation.

Ultraviolet rays are broadly classified into UVA (320-400 nm) and UVB (280-320 nm). In skin exposed to UVB, singlet oxygen ( ¹ O ₂ ), superoxide anion radical (O ₂ ^- ), hydrogen peroxide (H ₂ O ₂ ), hydroxyl radical ( OH), alkoxyl radical (OR) and peroxidation Various types of reactive oxygen species (ROS) such as radicals (· OOR) are generated. Reactive oxygen species are highly reactive molecules involved in various reactions such as intracellular damage and molecular signals. They not only initiate protein oxidation, DNA damage, and lipid peroxidation reactions, but also stimulate the secretion of matrix metalloproteinases (MMPs) to form extracellular matrix such as collagen or gelatin. It can induce decomposition and accelerate skin aging.

MMPs are divided into collagenase, gelatinase, and stromelysin according to their structural and functional properties. MMP-1 is known as collagenase Ⅰ, and type Ⅰ and Ⅲ collagen are used as substrates. MMP-3 is stromelysin Ⅰ, which uses type 4 collagen as a substrate to break down collagen into fragments. MMP-2 is a gelatinase that also plays a major role in skin aging by degrading the substrates constituting the skin dermis. Therefore, the development of a material that suppresses the expression of MMPs that decompose collagen and elastin, which are matrix substances constituting the skin, by suppressing the generation of excess active oxygen generated in the skin is evaluated as very important in functional cosmetics research.

A number of patents on cosmetic compositions containing plant extracts having a skin wrinkle improvement effect have been published. Korean Patent Registration No. 10-1574248 discloses a cosmetic composition for improving skin wrinkles containing an extract of Reynoutria sachalinensis as an active ingredient. In addition, Korean Patent Registration No. 10-1693931 discloses a cosmetic composition for improving wrinkles containing an extract of Scytosiphon lomentaria as an active ingredient.

The present invention, among several natural plant extracts, has excellent elasticity improvement ability compared to the prior art, and when extracts of Woobangja, Noni, Longan, and Sambaekcho are mixed while looking for a substance helpful in relieving skin wrinkles, skin elasticity enhancement and wrinkles It was confirmed that it can help improve.

Therefore, an object of the present invention is to provide a cosmetic composition for enhancing skin elasticity and improving wrinkles containing a mixture of extracts of Raisinia extract, Noni extract, Longan extract and Sambaekcho extract as active ingredients.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin wrinkles containing a mixed extract of Euphorbia extract, Noni extract, Longan extract and Sambaekcho extract as an active ingredient.

Preferably, the cosmetic composition has an effect of improving skin elasticity.

Also preferably, the Astragalus extract, Noni extract, Longan extract, and Sambaekcho extract may be contained in a weight ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10, and more preferably 1: 1: It is preferable to contain it in a weight ratio of 1:1.

Also preferably, the mixed extract is included in an amount of 0.01 to 30% by weight based on the total cosmetic composition.

Preferably, the cosmetic composition may be any one selected from the group consisting of nutrient lotion, nutrient essence and nutrient cream.

The cosmetic composition containing the mixed extract of Eubangja, Noni, Longan, and Sambaekcho extracts according to the present invention not only inhibits the activity of elastase, but also has an excellent anti-wrinkle effect because of its excellent inhibitory effect on MMP-1 production. In addition, the composition of the present invention containing a mixed extract of Eubangja, Noni, Longan, and Sambaekcho extracts is effective in improving skin elasticity, so it can be usefully used as a cosmetic for improving skin elasticity and wrinkles.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meanings commonly understood by those of ordinary skill in the art related to the present invention. In addition, although preferred methods or samples are described in this specification, those similar or equivalent thereto are also included in the scope of the present invention.

The term "about" means a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length of 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, quantity, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprise" and "comprising" include a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that steps or groups of components are not excluded.

The present invention provides a cosmetic composition for improving skin wrinkles containing a mixture of extracts of Euphorbia japonica extract, noni extract, longan extract, and triticale extract as an active ingredient.

Arctium lappa is the dried mature seed of burdock belonging to the Asteraceae family. Burdock is 50~150cm high, straight roots grow up to 30~60cm, and a stem comes out from the end. The outer surface is dark green, but white downy hairs grow densely on the back side, and there are tooth-shaped sawtooth edges. Flowers bloom in July-August, and are black purplish, and douhwa hangs in corymbose at the end of branches. The guns are round, the guns are needle-shaped, and the ends are like hooks. Flowers are tubular, seeds are black, and hairs are brown. The fruit is an achene and ripens in September. Burdock root is known to contain inulin and some palmitic acid. In Europe, it is used as a diuretic and diaphoretic, and the seeds are used as a diuretic when there is swelling, and as an antidote for sore throat and poisonous insects. The seed of burdock is called Ubangja and is also used as a medicinal material that has the effect of communicating wind heat, making it swollen, disposing of waste, sprouting papules, relieving swelling, and detoxifying.

In addition, Noni ( Morinda citrifolia ) is a medicinal plant native to Morocca Islands, Indonesia. From about 6,000 years ago, fruits and leaves have been used for food or medicine, and roots and stems have been used for dyeing. In Indonesia, it is called Menkd, Pache, Diva, etc., and as a medicine effective for all diseases, Jamura is still used as a traditional medicine.

In addition, longan ( Euphoria longana ) is a plant that grows in the tropics as an aphid plant. Leaves are alternate, thick and long oval, 15-45 cm long, with flat edges. Flowers are yellowish white in April, 3-6 cm in diameter, fragrant, and about 1,000 flowers hang on a panicle.

The three baekcho ( Saururus chinensis ) is a perennial herb that grows in some areas of Jeju Island and Jirisan Mountain. The growth environment is well-ventilated in a humid valley, and grows in a semi-shaded place with high air humidity. The height is 50-100 cm, the leaves are 5-15 cm long, 0.3-0.8 cm wide, long oval, and grow alternately. The surface of the leaf is light green and the back side is light white. When the flower blooms, 2-3 upper leaves turn white and have 5-7 veins, the tip is sharp and the edge is flat. The root is white, digs into the soil, and grows while extending to the side. The flower is white, droops downward, then the end part rises upward, faces the leaf, is 10 to 15 cm long, and has curly hairs. The fruit hangs in a round shape on a flower bud in September-October. It is used for ornamental purposes, and leaves including flowers and stems and roots are used as medicine.

In addition, according to one embodiment of the present invention, the present invention provides a cosmetic composition for enhancing skin elasticity containing a mixed extract of chinensis extract, noni extract, longan extract, and triticale extract as an active ingredient.

Since the extracts of Eupinia extract, Noni extract, Longan extract, and Sambaekcho extract alone have collagen production and collagenase inhibitory effects, they can be used alone as cosmetic products for improving wrinkles. However, since it has a more excellent collagen production effect and collagenase inhibitory effect when Astragalus extract, Noni extract, Longan extract and Sambaekcho extract are mixed, it is preferable to mix them together.

Preferably, the Eubangia extract, Noni extract, Longan extract and Sambaekcho extract may be contained in a weight ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10, more preferably 1: 1: 1: It is preferable to contain it in a weight ratio of 1. However, the content ratio may be mixed in various weight ratios depending on the characteristics of the product.

Each of the above extracts may be prepared according to a conventional method known in the art, that is, under conventional conditions of temperature and pressure, using a conventional solvent, but preferably (a) water and 1 to 4 carbon atoms. Anhydrous or hydrous lower alcohols (e.g. methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and a solvent extraction method using a solvent selected from the group consisting of mixtures thereof, (b) supercritical extraction method using carbon dioxide under reduced pressure and high temperature, or (c) ultrasonic extraction method. In the present invention, a solvent extraction method using an extraction solvent is preferably used.

In the present invention, the extract is a variety of extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (eg, methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol , It can be obtained using one or more extraction solvents selected from the group consisting of glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, and mixtures thereof, preferably ethanol, 70% (v/v) ethanol or water, most preferably 70% (v/v) ethanol. On the other hand, it is obvious to those skilled in the art that the extract of the present invention can be obtained using not only the above extraction solvent, but also other extraction solvents exhibiting substantially the same effect.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also an extract subjected to conventional purification and fermentation processes. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using an ultrafiltration membrane with a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity) Active fractions obtained through various additional purification and extraction methods, such as separation by a method for separation according to sex) or extracts obtained by natural conditions or fermentation products using various microorganisms, are also included in the extract of the present invention.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredients as active ingredients, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. adjuvants, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , It may be formulated into oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed with water. As specific examples, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream. , cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Hereinafter, the present invention will be described in more detail through non-limiting examples. However, the following examples are intended to illustrate the present invention, and the scope of the present invention is not to be construed as being limited by the following examples.

Preparation Example 1: Preparation of chinensis extract

After washing 100 g of Brussels sprouts with purified water, drying and crushing into small pieces, 70% lower alcohol in an amount of 1 to 10 times its dry weight is added thereto. After extracting at 15-35° C. for 5 days, the mixture was filtered with a 300 mesh filter cloth, again filtered with a Whatman No. 5 filter paper, and then dried with a rotary vacuum evaporator to obtain an extract having a dry weight of 28.7 g.

Preparation Example 2: Preparation of noni extract

After washing with purified water and crushing dried noni fruit, 100g was put into 1.2 ℓ of water, extracted at 4-40℃ for 5 days, filtered with a 300 mesh filter cloth, left at a low temperature of 3-5℃ for 5 days, and then aged with burnt filter paper. Filtered. This filtrate was dried with a rotary vacuum evaporator at 65°C to obtain a noni extract having a dry weight of 25.13 g.

Preparation Example 3: Preparation of longan extract

100g of Longan fruit washed with purified water and dried was put into 1.2L of water, extracted by heating at 70-90℃ for 5 hours in an extractor equipped with a cooling condenser, filtered through a 300 mesh filter cloth, and 7-10 days at 5-10℃. After aging by standing, it was filtered with Whatman No. 5 filter paper. This filtrate was dried with a rotary evaporator at 65°C to obtain a dry weight of 11.8 g.

Preparation Example 4: Preparation of Sambaekcho extract

After finely chopping the dried triticale leaves, put 100 g of dry weight in a flask, cool with 1 L of purified water, extract using a high-pressure sterilizer (121 ° C, 15 minutes, 1.5 atm), and filter to remove the residue. was manufactured.

Preparation Examples 5 to 15: Mixed extract preparation of Eubangia extract, Noni extract, Longan extract and Sambaekcho extract

Extracts of Woobangja, Noni, Longan, and Sambaekcho were mixed in a weight ratio as shown in Table 1 below to obtain a mixed extract. Specifically, after dissolving each of the extracts obtained in Preparation Examples 1 to 4 in tertiary distilled water at a concentration of 20 mg/ml, a mixed extract was prepared by mixing the aqueous extracts so that the weight ratio of each extract was in Table 1.

manufacturing example right side noni longan three hundred seconds 5 50 50 0 0 6 50 0 50 0 7 50 0 0 50 8 0 50 50 0 9 0 50 0 50 10 0 0 50 50 11 33.3 33.3 33.3 0 12 33.3 33.3 0 33.3 13 33.3 0 33.3 33.3 14 0 33.3 33.3 33.3 15 25 25 25 25

Preparation Examples 16 to 18: Preparation of cosmetics containing mixed extracts

When the mixed extract of the present invention was applied directly to human skin, cosmetics of Preparation Examples 16 to 18 were prepared with the composition shown in Table 2 in order to confirm whether they had excellent effects.

Raw material name (% by weight) Preparation Example 16 Preparation Example 17 Preparation Example 18 Comparative Preparation Example 1 Preparation Example 11 10 - - - Production Example 14 - 10 - - Production Example 15 - - 10 - glycerin 2.8 2.8 2.8 2.8 Polyglyceryl-3 Methylglucose Distearate 1.6 1.6 1.6 1.6 PEG-4 Olivate 0.4 0.4 0.4 0.4 cetearyl alcohol 0.2 0.2 0.2 0.2 squalane 4.5 4.5 4.5 4.5 Caprylic/Capric Triglyceride 4.2 4.2 4.2 4.2 dimethicone 1.3 1.3 1.3 1.3 Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer 1.0 1.0 1.0 1.0 incense a very small amount a very small amount a very small amount a very small amount Distilled water balance balance balance balance

Experimental Example 1: Cytotoxicity evaluation

To confirm cytotoxicity, fibroblast NHDF (normal human dermal fibroblast) was cultured in DMEM medium supplemented with 10% FBS (fetal bovine serum), and inoculated in a 96 well plate at a cell concentration of 3×10 ⁴ cells/well. 37 ℃, 5% CO ₂ It was cultured for 24 hours in an incubator. After culturing, the medium was removed and the concentration of the extract prepared in the above preparation was diluted in DMSO (dimethyl sulfoxide) to a concentration of 1.0%. After culturing for 24 hours, MTT (3-[4,5-dimethylthiazol -2yl] -2,5-diphenyltetrazolium boromide, Sigma, USA) solution was added to each well by 100 μl (3 mg/ml) and further incubated for 4 hours. Thereafter, the supernatant was removed, 150 μl of DMSO was added, shaken for 30 minutes to dissolve the generated formazan, and absorbance was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max190). Cell viability was calculated according to the formula below, and the results are shown in Table 3 below.

test substance Cell viability (%) control group 100 Preparation Example 1 99.6 Preparation Example 2 99.6 Preparation Example 3 99.8 Production Example 4 99.5 Preparation Example 5 99.5 Preparation Example 6 99.8 Preparation Example 7 99.4 Preparation Example 8 98.4 Preparation Example 9 98.4 Production Example 10 99.2 Preparation Example 11 99.5 Production Example 12 99.4 Preparation Example 13 98.9 Production Example 14 99.0 Production Example 15 99.6

Referring to Table 3, when all samples applied with extracts of Woobangja, Noni, Longan, or Sambaekcho, or a mixture extract thereof, showed a cell viability of 95% or more when treated with NHDF, the cytotoxicity of human skin-derived fibroblasts was affected. , and it was confirmed that there is no problem with safety.

Experimental Example 2: MMP-1 production inhibitory effect

Human skin-derived NHDF cells were inoculated into a 48-well plate (Nunc, Denmark) to be 1×10 ⁶ cells per well, cultured for 24 hours in a DMEM medium and 37° C., 5% CO ₂ incubator, and then prepared in Preparation Example 1 to In a serum-free DMEM medium to which the final concentration of the extract of No. 15 and these mixed extracts was added to be 1.0%, and as a control, cultured for 48 hours in a serum-free DMEM medium that did not contain extracts of Raisinia extract, Noni extract, Longan extract, and Sambaekcho extract. did After culturing, the supernatant of each well was collected and the amount (ng/ml) of newly synthesized MMP-1 was measured using an MMP-1 assay kit, and the inhibition rate of MMP-1 production was calculated according to Equation 2 below, The results are shown in Table 4. At this time, TGF-β (10 ng/ml, Roche, USA) was used as a positive control for the inhibition of MMP-1 production.

test substance MMP-1 production inhibition rate (%) TGF-β (10 ng/ml) 72.1 Preparation Example 1 70.2 Preparation Example 2 72.8 Preparation Example 3 70.6 Production Example 4 71.1 Preparation Example 5 76.1 Preparation Example 6 75.5 Preparation Example 7 73.2 Preparation Example 8 77.6 Production Example 9 76.2 Production Example 10 75.3 Preparation Example 11 82.5 Production Example 12 72.7 Preparation Example 13 73.8 Production Example 14 81.9 Production Example 15 86.1

Referring to Table 4, when comparing the extracts of Woobangja, Noni, Longan, or Sambaekcho and their mixed extracts in Preparation Examples of the present invention, Preparation Examples 11, 14, and 15, which are mixed extracts, produce excellent MMP-1 in human fibroblasts. inhibition rate was shown. In particular, Preparation Example 15 showed the best activity. Here, MMP-1 is a collagen degrading enzyme, and is a protein whose expression is increased by stress caused by various external stimuli (ultraviolet rays). When exposed to ultraviolet rays, its synthesis increases in skin cells, and it dissolves and decomposes skin matrix proteins, and external stimuli including ultraviolet rays further increase MMP-1 enzyme and synthesis, thereby accelerating aging. The suppression of the production of this MMP-1 enzyme means that it is effective in improving skin wrinkles.

Experimental Example 3: Procollagen type 1 production increasing effect

Collagen is a structural protein mainly found in fibrous tissues such as tendons, ligaments and skin, and type 1 accounts for about 90% of the total collagen. In the skin, it is responsible for wrinkle improvement, elasticity, and skin damage recovery. The present inventors inoculated NHDF cells into a 24-well plate and cultured them in a 37°C, 5% CO ₂ incubator for 24 hours in order to confirm the effect of increasing the production of procollagen type 1 in fibroblasts. Then, the extract prepared in Preparation Example was treated so that the sample concentration was 1.0%, and then cultured for 24 hours. Thereafter, the medium of the cultured cells was collected and the amount of procollagen type 1 was measured using the Procollagen type I cpeptide EIA kit (TAKARA, Japan). On the other hand, the cells attached to the bottom were washed with PBS and then dissolved with 1N NaOH to measure the total amount of protein, and the amount of pre-collagen production per certain protein was calculated.

The results are shown in Table 5 below.

test substance Procollagen production/total protein Preparation Example 1 5.9 Preparation Example 2 6.3 Preparation Example 3 5.9 Production Example 4 6.1 Preparation Example 5 6.6 Preparation Example 6 6.4 Preparation Example 7 6.4 Preparation Example 8 6.8 Preparation Example 9 6.6 Production Example 10 6.4 Preparation Example 11 7.4 Production Example 12 6.2 Preparation Example 13 6.3 Production Example 14 7.3 Production Example 15 7.8

Referring to Table 5, when comparing the extracts of Woobangja, Noni, Longan, or Sambaekcho of the present invention and their mixed extracts, Preparation Examples 11, 14, and 15, which are mixed extracts, produced procollagen type 1 in human fibroblasts was found to increase effectively. In particular, Preparation Example 15 showed the best activity.

Experimental Example 4: Skin wrinkle improvement effect

The skin wrinkle improvement effect of the cosmetic composition containing the mixed extract of Eubangia extract, Noni extract, Longan extract and Sambaekcho extract of the present invention was measured on 40 healthy women aged 30 to 50 years old. First, it is divided into four groups, Preparation Example 16 in Group A, Preparation Example 17 in Group B, Preparation Example 18 in Group C, and Comparative Preparation Example 1 in Group D are applied to the face twice a day (morning and evening) 3 applied for months. After 3 months, the degree of wrinkle improvement was evaluated through image analysis of wrinkles.

For the evaluation of wrinkles by image analysis, a replica is taken under the eyes before the experiment begins, and a replica is taken from the same area under the eyes immediately after the experiment is finished, and the density of wrinkles is analyzed by two-dimensional analysis of wrinkles through image analysis. was measured. The wrinkle density measurement results by image analysis are shown in Table 6 below as a reduction rate for wrinkle density compared to before use.

sample Wrinkle density reduction rate (%) Preparation Example 16 35.5 Preparation Example 17 33.8 Preparation Example 18 50.1 Comparative Preparation Example 1 (Control) 15.6

From Table 6, in the case of Preparation Example 18 containing the mixed extract of Woobangja, Noni, Longan and Sambaekcho extracts, it was found that the skin wrinkle improvement effect was very excellent compared to the control group.

Experimental Example 5: Skin elasticity improvement effect

In order to test the effect of improving elasticity in real human skin, the following test was performed. 40 women between the ages of 30 and 50 were divided into 4 groups of 10 each and applied to the face twice daily in the morning and evening for 8 weeks. The skin elasticity improvement effect was measured, and the results are shown in Table 7 below. The result values in Table 7 mean the properties of skin elasticity (viscoelasticity) of the skin elasticity tester. In the case of Preparation Example 18, it was confirmed that the skin elasticity effect was significantly improved compared to the control group.

sample Skin elasticity improvement rate (%) Preparation Example 16 10.5 Preparation Example 17 10.2 Preparation Example 18 17.0 Comparative Preparation Example 1 (Control) 2.4

Experimental Example 6: Primary skin irritation evaluation (patch test)

During this human application test period, the test subject visited a total of three times (before the start of the test, on the day of patch removal, and after 30 minutes after removing the patch) to determine whether or not skin irritation was present. At the first visit, the test subject performed a skin patch test on the subject's back using a Finn chamber (100 × 10, EPITEST, Finland). For 20 test subjects, samples were applied between the shoulder blades and the waistline centering on the back spine using a pin chamber.

After wiping the test site with 70% ethanol and drying it, 20 μl of each sample was dropped into the pin chamber, and then placed on the test site and fixed. It was applied to the skin for 24 hours, and after removing the patch, the test site was marked with a marking pen, and after 30 minutes, erythema and edema of the test site were observed using a magnifying glass (8MC-150, DAZOR, USA).

The skin reaction was determined according to the regulations (Table 8) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated by Equation 2 below, and the results are shown in Table 9.

- unresponsive 0 . faint mild erythema 0.5 ± Mild erythema with well-defined borders One + Distinct erythema, papules and vesicles 2 ++ severe erythema, bulla 6 +++ crust formation 12

The degree of skin irritation in Experimental Example 1 was shown according to the evaluation criteria as shown in Table 2 above, and the skin irritation rate was obtained by the calculation formula below and shown in Table 3.

[Equation 3]

For skin irritation, if the irritation rate is 0.0, it is non-irritating, if it is 0.1~1.0, it is not irritating, if it is 1.0~1.7, it is mild irritation, if it is 1.7~2.5, it is medium stimulation, and if it is 2.5 or more, it is judged as strong irritation.

sample skin irritation rate stimulation Preparation Example 16 0.00 non-irritating Preparation Example 17 0.00 non-irritating Preparation Example 18 0.00 non-irritating Comparative Preparation Example 1 (Control) 0.00 non-irritating

Looking at Table 9, it was confirmed that the cosmetic composition containing the mixed extract of the present invention did not cause skin irritation and had excellent skin stability.

Claims (5)

A cosmetic composition for improving skin wrinkles, comprising a mixture of extracts of bellflower extract, noni extract, longan extract, and sambaekcho extract as an active ingredient. According to claim 1,
The mixed extract is a cosmetic composition for improving skin wrinkles, characterized in that the weight ratio of bellflower extract, noni extract, longan extract and triticale extract is mixed in a weight ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10. According to claim 1,
The mixed extract is a cosmetic composition for improving skin wrinkles, characterized in that contained 0.01 to 30% by weight based on the total weight of the cosmetic composition. According to claim 1,
The cosmetic composition is a cosmetic composition for improving skin wrinkles, characterized in that it has a skin elasticity improvement effect. According to claim 1,
The cosmetic composition is any one selected from the group consisting of nutrient lotion, nutrient essence and nutrient cream, a cosmetic composition for improving skin wrinkles.