KR20230072268A - Cosmetic composition comprising yuja seed oil - Google Patents

Abstract

The present invention relates to a cosmetic composition containing citron seed oil, and more specifically, to a cosmetic composition containing citron seed oil extracted by a cold-pressing method and thus exhibiting excellent effects in at least one of anti-inflammatory, anti-allergic and moisturizing effects.

Description

Cosmetic composition containing citron seed oil {COSMETIC COMPOSITION COMPRISING YUJA SEED OIL}

The present invention relates to a cosmetic composition containing citron seed oil, and more specifically, to a cosmetic composition containing citron seed oil extracted by a cold-pressing method and thus exhibiting excellent effects in at least one of anti-inflammatory, anti-allergic and moisturizing effects.

External stimuli such as chemical irritants, environmental pollution, and ultraviolet rays cause inflammatory allergic diseases such as acne, atopic dermatitis, melasma, wrinkles, etc., and various functions such as immunity and skin elasticity are reduced.

When drugs such as anti-inflammatory drugs and antihistamines are used to solve these problems, symptoms may be temporarily improved, but various side effects occur, making it difficult to take them for a long time. Therefore, methods that can utilize natural products instead of drugs are being developed.

Citron contains an excessive amount of ingredients such as vitamin C and citric acid, so it is known to be effective in enhancing immunity, skin whitening, antioxidant, and anti-atopy. However, citron has a problem in that it is difficult to process due to its thick skin and many seeds, and most of the citron seeds are not efficiently used, such as being discarded.

The present invention is to solve the above problems, and an object of the present invention is to provide at least one effect of anti-inflammatory, anti-allergic and moisturizing by including citron seed oil in a cosmetic composition.

The technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the description below.

According to an embodiment of the present invention, a cosmetic composition containing citron seed oil can be provided.

In addition, the citron seed oil may be included in an amount of 0.01% to 10% by weight based on the total weight of the composition.

In addition, the citron seed oil can be extracted by cold pressing.

In addition, the cosmetic composition may have excellent at least one effect of anti-inflammatory, anti-allergic and moisturizing.

In addition, the cosmetic composition may be any one formulation selected from the group consisting of skin, lotion, serum, essence, mask pack, sunscreen, BB cream, nutrient cream, moisture cream, massage cream, and cleansing cream.

According to another embodiment of the present invention, a method for extracting citron seed oil is provided. The method of the present invention comprises the steps of separating and collecting seeds from citron fruits; Drying the seeds at a temperature of 55 to 60 ° C. so that the moisture content of the seeds is 20% by weight or less; Grinding the seed to prepare a powder; and extracting the powder into oil using a hydraulic oil pump.

The cosmetic composition according to the present invention is effective in anti-inflammation and anti-allergy by including citron seed oil, and has an effect of improving moisturizing power.

In addition, since the cosmetic composition according to the present invention is prepared using citron seeds separated from yuzu, it has the advantage of efficiently utilizing citron seeds that have been previously discarded.

In order to more fully understand the drawings cited in the detailed description of the present invention, a brief description of each drawing is provided.
1 is a view related to cell viability experiments for macrophages of citron seed oil according to Preparation Examples 1 to 9 of the present invention.
2 is a diagram related to cell viability experiments for mast cells of citron seed oil according to Preparation Examples 1 to 9 of the present invention.
Figure 3 is a view related to the anti-inflammatory effect test of citron seed oil according to Preparation Examples 1 to 3 of the present invention.
Figure 4 is a view related to the anti-allergic effect test of citron seed oil according to Preparation Examples 1 to 3 of the present invention.
Figure 5 is a view related to the moisture content during the moisturizing effect test of the cosmetic composition according to Example 2 and Comparative Example 2 of the present invention.
Figure 6 is a view related to the moisture improvement rate during the moisturizing effect test of the cosmetic composition according to Example 2 and Comparative Example 2 of the present invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is one well known and commonly used in the art. In addition, in describing an embodiment of the present invention, if it is determined that a detailed description of a related known configuration or function hinders understanding of the embodiment of the present invention, the detailed description will be omitted. In addition, embodiments of the present invention will be described below, but the technical idea of the present invention is not limited or limited thereto and can be modified and implemented in various ways by those skilled in the art.

The cosmetic composition of the present invention may contain citron seed oil.

Citron seed oil is composed of unsaturated fatty acids such as oleic acid and linoleic acid, which can provide excellent moisturizing effects, and is composed of components such as vitamin C and citric acid, which play a role in providing anti-inflammatory and anti-allergic effects. can

According to embodiments, citron seed oil may be included in an amount of 0.01% to 10% by weight, preferably 1 to 5% by weight, based on the total weight of the composition. When citron seed oil is contained in less than 0.01% by weight or more than 10% by weight, anti-inflammatory, anti-allergic or moisturizing effects may be reduced.

Depending on the embodiment, citron seed oil extracted by cold pressing may be used. When a cosmetic composition is prepared using citron seed oil extracted by the cold-pressing method, the content of fatty acids increases compared to citron seed oil extracted by the hot-pressing method, and the fatty acids promote moisture penetration into the skin to moisturize the skin. effect can be maximized. However, the extraction method of citron seed oil is not limited thereto, and all known methods for extracting oil may be applied.

The present invention uses the seeds of the citron, rather than the rind or pulp of the citron used conventionally, and has the advantage of efficiently utilizing the citron seeds that have been previously discarded.

Depending on the embodiment, the cosmetic composition may be oil-in-water (O/W) or water-in-oil (W/O), but all formulations are possible, and may include oil and water phases.

The oil phase can control the overall viscosity, spreadability, and adhesion of the cosmetic composition.

According to an embodiment, the oil phase may include citron seed oil, and may further include at least one of PEG-100 Stearate and Glyceryl Stearate. However, it is not limited thereto.

The aqueous phase can dissolve water-soluble components and impart a moisturizing effect and a refreshing feeling to the cosmetic composition.

Depending on the embodiment, the aqueous phase may include at least one of purified water, glycerine, carbomer, and sclerotium gum. However, it is not limited thereto.

Depending on the embodiment, the cosmetic composition may exhibit excellent at least one effect of anti-inflammatory, anti-allergic and moisturizing. This is because the active ingredients of citron seeds, including citron seed oil, can be efficiently obtained.

According to the embodiment, the cosmetic composition may be formulated into any one formulation selected from the group consisting of skin, lotion, serum, essence, mask pack, sunscreen, BB cream, nourishing cream, moisture cream, massage cream, and cleansing cream. there is. Preferably, it may be formulated into a formulation for skin care. However, it is not limited thereto.

According to the embodiment, the cosmetic composition is a conventional cosmetic such as a conditioning agent, powder, thickener, pigment, film former, nutrient, volatile solubilizer, antioxidant, preservative, and fragrance within the range of not impairing the effects of the present invention. Other active ingredients contained in the composition may be further included. However, it is not limited thereto.

Citron seed oil of the present invention can be extracted through the following method. First, seeds are separated and collected from citron fruits, and the seeds may be dried at a temperature of 55 to 60° C. so that the moisture content of the seeds is 20% by weight or less. Thereafter, the seeds are pulverized to produce a powder, and then the powder can be extracted into oil using a hydraulic oil press. When a cosmetic composition is prepared using citron seed oil extracted by such a cold-pressing method, at least one of anti-inflammatory, anti-allergic and moisturizing effects can be maximized. However, it is not limited thereto, and all known methods of extracting oil may be applied.

[Experimental example]

Hereinafter, examples for explaining the present invention in more detail are shown as follows, but the present invention is not limited thereto.

Component analysis of citron seed oil

(1) Preparation of Example 1 and Comparative Example 1

Examples and comparative examples of citron seed oil according to the present invention were prepared. The specific manufacturing method is as follows.

Citron seeds separated from citron fruits were washed and dried, and then milked at 65° C. with a low-temperature press to prepare citron seed oil of Example 1. Comparative Example 1 is citron seed oil prepared by milking washed and dried citron seeds at 200 to 300° C. with a high-temperature press.

(2) Experimental Example 1: Component analysis of citron seed oil

In order to analyze the components of citron seed oil according to Example 1 and Comparative Example 1, 2 mL of N-Hexane and 2 mL of catalyst solution were mixed with 50 μL of citron seed oil, followed by 10 hours in freezing conditions. left unattended. Thereafter, the supernatant was transferred to a vial for analysis, and component analysis was performed on citron seed oil.

As a result of the experiment, in the case of Example 1, a total of five fatty acids were measured, and linoleic acid, oleic acid, stearic acid, and palmitic acid were measured as major fatty acids. Specifically, 32.2 ± 0.72% of linoleic acid, 40.8 ± 0.51% of oleic acid, 7.30 ± 9.33% of stearic acid, and 16.2 ± 0.21% of palmitic acid were measured, and it was confirmed that 23.50% of saturated fatty acids and 74.00% of unsaturated fatty acids were included. there was.

On the other hand, in the case of Comparative Example 1, linoleic acid 29.33 ± 2.39%, oleic acid 24.14 ± 2.28% and palmitic acid 14.20 ± 2.20% were measured, and the main fatty acids were included as 67.67% with respect to the total fatty acids. This is a 22.5% decrease in the content of main fatty acids compared to Example 1, and it was confirmed that the content of fatty acids contained in Comparative Example 1, in which citron seed oil was extracted by the hot pressing method, was smaller than that of Example 1.

Effect confirmation experiment of citron seed oil

(1) Production of Production Examples 1 to 9

Preparation examples of citron seed oil according to the present invention were prepared according to the concentrations shown in Table 1 below. The specific manufacturing method is as follows.

After washing 500 g of citron seeds collected by separating only the seeds from the citron fruits, the seeds were dried in an air dry condition at 60° C. to adjust the moisture content to within the range of 20% by weight, and then the seeds were pulverized to prepare a powder. Thereafter, the powder was put into a hydraulic press to extract seed oil, and filtered through a filter to prepare citron seed oil.

manufacturing example Citron seed oil concentration (%) manufacturing example Citron seed oil concentration (%) Preparation Example 1 0.05 Preparation Example 5 10 Preparation Example 2 0.01 Preparation Example 6 5 Preparation Example 3 0.005 Preparation Example 7 One Production Example 4 0.001 Preparation Example 8 0.5 Preparation Example 9 0.1

(2) Experimental Example 2: Confirmation of cell viability of citron seed oil

cells/well로, 24 well plate에 비만세포(RBL-2H3)를 2 ×

cells/well로 분주하고 24시간동안 배양한 후, 유자씨 오일을 첨가하여 24시간동안 처리하였다. 이후, 최종농도가 5mg/mL이 되도록 MTT 용액을 넣고 37℃, 5% CO²배양기에서 3시간동안 반응시킨 뒤, microplate reader를 이용하여 570nm에서의 흡광도를 측정하여 세포생존율을 측정하였으며, 그 결과를 도 1 및 도 2에 나타내었다. 구체적으로, 도 1은 대식세포에 대한 유자씨 오일의 세포생존율의 결과이며, 도 2는 비만세포에 대한 유자씨 오일의 세포생존율의 결과이다.In order to measure the cell viability of the citron seed oil according to Preparation Examples 1 to 9, macrophages (RAW 264.7) were 1 ×

cells/well, mast cells (RBL-2H3) were plated in 24 well plates by 2 ×

After dividing into cells/well and incubating for 24 hours, citron seed oil was added and treated for 24 hours. Thereafter, MTT solution was added to a final concentration of 5 mg/mL, reacted for 3 hours in a 37°C, 5% CO² incubator, and cell viability was measured by measuring absorbance at 570 nm using a microplate reader. 1 and 2 are shown. Specifically, FIG. 1 is a result of cell viability of citron seed oil on macrophages, and FIG. 2 is a result of cell viability of citron seed oil on mast cells.

As a result of the experiment, in the case of Preparation Examples 1 to 4, it was confirmed that the cytotoxicity was not affected as cell viability of 90% or more was measured for both macrophages and mast cells.

On the other hand, in the case of Preparation Examples 5 to 9, it was confirmed to exhibit cytotoxicity as low cell viability was measured for both macrophages and mast cells.

(3) Experimental Example 3: Confirmation of anti-inflammatory effect of citron seed oil

cells/well에 리포 다당류(Lipopolysaccharide, LPS)를 1μg/mL 농도로 처리하고, 유자씨 오일을 첨가하여 24시간동안 배양하였다. 이후, 배양 상층액 100μL와 griess reagent 100μL을 혼합하고 540nm에서 흡광도를 측정하여 염증 유발자인 산화질소(Nitric Oxide, NO)의 생성량을 측정하였으며, 그 결과를 도 3에 나타내었다.Macrophages (RAW 264.7) 1 ×

Cells/well were treated with lipopolysaccharide (LPS) at a concentration of 1 μg/mL, and citron seed oil was added and cultured for 24 hours. Thereafter, 100 μL of the culture supernatant and 100 μL of the griess reagent were mixed and the absorbance was measured at 540 nm to measure the production of nitric oxide (Nitric Oxide, NO), an inflammation inducer, and the results are shown in FIG. 3 .

As a result of the experiment, in the case of Preparation Examples 1 to 3, it was confirmed that the anti-inflammatory effect occurred by suppressing the production of NO, which is an inflammation inducer, by 53%, 12% and 5%, respectively, and inhibiting the production of NO in a concentration-dependent manner confirmed.

(4) Experimental Example 4: Confirmation of anti-allergic effect of citron seed oil

cells/well로 분주하고 37℃, 5% CO²조건에서 24시간동안 반응시킨 후, 인산완충생리식염수(Phosphate buffered saline, PBS)를 이용하여 세척하였다. 이후, anti-DNP-IgE 50ng/mL를 처리하여 24시간동안 배양하고, MEM 배지로 2회 세척하고 37℃에서 20분간 배양한 뒤, 유자씨 오일과 1μg/mL 농도의 DNP-BSA 20μL을 첨가하였다. 그후, 세포가 과립을 형성하도록 37℃에서 1시간동안 반응시켜 생성된 상측액 25μL에 0.1M Citrate buffer(with 10mM p-nitropheny-N-acetyl-β-D-glucosaminide, pH 4.5) 50μL을 첨가하여 37℃에서 1시간동안 반응시켰다. 마지막으로, microplate reader를 이용하여 405nm에서 흡광도를 측정하여 알러지반응이 나타날 때 분비되는 물질인 β-hexosaminidase의 방출 억제력을 측정하였으며, 그 결과를 도 4에 나타내었다.In order to confirm the anti-allergic effect of citron seed oil according to Preparation Examples 1 to 3, mast cells (RBL-2H3) were cultured in 24 well plates in 2 ×

Cells/well were dispensed and reacted for 24 hours at 37°C and 5% CO², followed by washing with phosphate buffered saline (PBS). Thereafter, treated with 50ng/mL of anti-DNP-IgE and cultured for 24 hours, washed twice with MEM medium, incubated at 37°C for 20 minutes, and then added with citron seed oil and 20μL of DNP-BSA at a concentration of 1μg/mL. did Then, 50 μL of 0.1M Citrate buffer (with 10 mM p-nitropheny-N-acetyl-β-D-glucosaminide, pH 4.5) was added to 25 μL of the supernatant produced by reacting the cells at 37° C. for 1 hour to form granules. Reacted at 37°C for 1 hour. Finally, the absorbance was measured at 405 nm using a microplate reader to measure the release inhibition of β-hexosaminidase, a substance secreted when an allergic reaction occurs, and the results are shown in FIG. 4 .

As a result of the experiment, in the case of Preparation Examples 1 to 3, it was confirmed that the anti-allergic effect occurred as the release inhibition of β-hexosaminidas, a substance secreted when an allergic reaction appeared, was measured at 26%, 12% and 2%, respectively. , it was confirmed that the release of β-hexosaminidas was inhibited in a concentration-dependent manner.

Effect confirmation test of cosmetic composition containing citron seed oil

(1) Preparation of Example 2 and Comparative Example 2

Examples and comparative examples of cosmetic compositions according to the present invention were prepared using the components listed in Table 2 below. The unit of component content in Table 2 is % by weight.

Example 2 is a composition containing citron seed oil, and Comparative Example 2 is a composition in which caprylic/capric triglyceride is added instead of citron seed oil in Example 2.

role ingredient Example 2 Comparative Example 2 Prime Minister Purified water balance skrelotium gum 1.0 1.0 carbomer 0.5 0.5 glycerin 7.0 7.0 pay-per-view citron seed oil 5.0 - PEG-100 Stearate 0.5 0.5 glyceryl stearate 0.5 0.5 Caprylic/Capric Triglyceride - 5.0 total 100 100

The specific manufacturing method is as follows.

After mixing the water phase part using an agi mixer, warming the oil phase part to about 75 ℃, and then putting the oil phase part into the water phase part and using a homo mixer at about 3,000 rpm for 5 minutes at about 75 ℃ Pre-emulsified while stirring. Thereafter, the emulsification was stirred at about 75° C. for 5 minutes at about 6,000 rpm, and cooled to about 35° C. to prepare the cosmetic composition of Example 2. Comparative Example 2 was prepared in the same process by adding caprylic/capric triglyceride instead of citron seed oil in the above process.

(2) Experimental Example 5: Confirmation of moisturizing effect of citron seed oil

In order to evaluate the effect of moisturizing with respect to the cosmetic composition according to Example 2 and Comparative Example 2, the moisture content and moisture improvement rate were measured.

The experiment method is as follows. The samples of Examples and Comparative Examples were applied to 20 women in their 20s to 60s by dividing the face in half centering on the nose, and measuring the moisture content of the skin surface before use, 1 week and 2 weeks after use using a corneometer. After measuring 5 times, the average value of the three values excluding the maximum and minimum values of the measured values was obtained, and the results are shown in FIG. 5 .

Moisture improvement rate means the degree of moisture improvement after 1 week and 2 weeks of use compared to before product use, and was derived according to the equation below, and the results are shown in FIG. 6.

Moisture improvement rate (%) = (measured value after product use - measured value before product use) / measured value before product use × 100

As a result of the experiment, in the case of Example 2, in the first week after using the composition, the skin moisture improvement rate was measured slightly lower than before use, but in the second week, it was confirmed that the skin moisture improvement rate increased by 3.90% compared to before use.

On the other hand, in the case of Comparative Example 2, the skin moisture improvement rate increased compared to before use in the first week after using the composition, but in the second week, the skin moisture improvement rate increased by 3.01% compared to before use, and the moisturizing effect was improved compared to Example 2. found to be degraded.

As above, specific parts of the content of the present invention have been described in detail, and for those skilled in the art, these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. It will be clear. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

In the cosmetic composition,
A cosmetic composition comprising citron seed oil. According to claim 1,
The citron seed oil is characterized in that contained in 0.01% to 10% by weight relative to the total weight of the composition, cosmetic composition. According to claim 1,
The citron seed oil is characterized in that extracted by a cold pressing method, a cosmetic composition. According to claim 1,
The cosmetic composition, characterized in that at least one of anti-inflammatory, anti-allergic and moisturizing effect is excellent, cosmetic composition. According to claim 1,
Characterized in that the cosmetic composition is any one formulation selected from the group consisting of skin, lotion, serum, essence, mask pack, sunscreen, BB cream, nutrient cream, moisture cream, massage cream, and cleansing cream, the cosmetic composition . Separating and collecting seeds from citron fruits;
Drying the seeds at a temperature of 55 to 60 ° C. so that the moisture content of the seeds is 20% by weight or less;
Grinding the seed to prepare a powder; and
Extracting the powder into oil using a hydraulic oil press.

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