KR20230067172A - Cosmetic composition for skin regeneration and anti-wrinkle comprising the essential oil derived from Kerria japonica f. pleniflora (Witte) Rehder Flower as an active ingredient - Google Patents

Abstract

The present invention identifies the components of essential oil extracted from the flower of Kerria japonica f. pleniflora (Witte) Rehder, which induces proliferation of epidermal keratinocytes and type I and IV collagen synthesis, The present invention was completed by confirming that the sprout out growth of the in vitro artificial skin model was induced in a concentration-dependent manner, inducing skin tissue regeneration, and exhibiting anti-wrinkle activity through enhancement of skin elasticity. Therefore, it may be used as a cosmetic composition or an external composition for skin.

Description

Cosmetic composition for skin regeneration and anti-wrinkle comprising the essential oil derived from Kerria japonica f. pleniflora (Witte) Rehder Flower as an active ingredient}

The present invention relates to a composition for skin regeneration and wrinkle improvement comprising Kerria japonica f . pleniflora (Witte) Rehder Flower essential oil as an active ingredient. More specifically, it relates to a cosmetic composition for skin regeneration and wrinkle improvement containing, as an active ingredient, essential oil extracted from flowers of Jukdanhwa by organic solvent extraction.

Skin is the outermost tissue of the human body and is composed of epidermis, dermis, and hypodermis. Among them, 95% of the epidermal layer is composed of keratinocytes, and depending on the degree of differentiation of keratinocytes, the basal layer, the prickle layer, the spinous layer, the granular layer, and the stratum corneum ( horny layer, cornified layer, stratum corneum). Keratinocytes efficiently defend against bacteria and chemicals penetrating into the skin and secrete various cytokines to participate in inflammatory and immune responses of the skin. In addition, migration and proliferation of keratinocytes induce wound healing, skin regeneration, and collagen synthesis to play an important role in enhancing skin elasticity and forming a basement membrane. There are more than 20 types of collagen according to the alpha chain, and type I, III, IV, V, VI and VII collagen are distributed in human skin. Mainly, type I collagen constitutes more than 85% of the dermal layer of the skin, and type IV collagen is the main structural protein of the basement membrane. It is known that the synthesis of type I and IV collagen enhances skin elasticity and improves basement membrane formation, thereby regulating the adhesion and proliferation of skin cells. In addition, keratinocytes produce basement membrane components while secreting collagen, cytokines, and the like, and migrate to the damaged skin area while cell proliferation proceeds. Therefore, proliferation and migration of keratinocytes and induction of collagen biosynthesis not only regenerate skin tissue through re-epithelialization of skin tissue, but also improve skin elasticity to improve wrinkles and help maintain healthy skin.

"Essential oil" derived from natural products is a general term for aromatic oils (fragrant and volatile substances obtained from plant leaves, stems, fruits, flowers, roots, etc.), and is classified in various ways according to extracts, extraction methods, uses and purposes. Essential oils derived from natural products are classified into essential oils, absolutes, and concretes according to extraction methods. The aromatic components of essential oils derived from natural products have effects such as soothing and relieving tension through the skin, circulatory system, and olfactory system, and have therapeutic effects such as antibacterial action, immunity enhancement, and anti-allergic action. It is also used as a raw material for food flavoring and cosmetics.

On the other hand, Jukdanhwa ( Kerria japonica f. pleniflora (Witte) Rehder) is a variety of yellow plum belonging to the genus Kerria and is characterized by double flowers. Jukdanhwa has strong solar resistance, so it grows well regardless of the sun and shade, and has strong resistance to cold and pollution, but it is difficult to grow in places with frequent sea breezes due to its weak salt resistance. Jukdanhwa is a bush-type shrub that grows well regardless of the sun or shade, and has been widely used as a landscaping tree for a long time because of its long flowering period and the beautiful appearance of dark yellow flowers covering the entire bush. The branches and stems of Jukdanhwa are always green, and the flowers are double flowers, blooming yellow in April-May, and blooming one at each end of the side branches. The place of origin is China and Japan, and in Korea, it grows naturally in the south of the central region. In oriental medicine, the flowers, stems, and leaves of Jukdanhwa are all used as a herbal medicine called 'Chedanghwa'. am.

Accordingly, the present inventors found that the essential oil derived from the flower of Jukdanhwa extracted using an organic solvent extraction method induces the proliferation of keratinocytes and the synthesis of type I and IV collagen, and sprouts out growth of a keratinocyte-based in vitro artificial skin model. The present invention was completed by confirming that it promotes.

10-2009-0059106

In order to solve the above problems, the present invention extracts essential oil from Kerria japonica f . To confirm, to provide a cosmetic composition for skin regeneration or wrinkle improvement.

In order to solve the above problems, the present invention provides a cosmetic composition for skin regeneration or wrinkle improvement containing essential oil extracted from Kerria japonica f. pleniflora (Witte) Rehder as an active ingredient.

In one embodiment of the present invention, the essential oil may be characterized in that it is extracted from bamboo flowers, but is not limited thereto.

In an embodiment of the present invention, the flower of Kerria japonica f. pleniflora (Witte) Rehder may not be dried.

In one embodiment of the present invention, the essential oil is extracted with hexane (n-hexane) and concentrated under reduced pressure to obtain solid concrete, and the solid concrete is extracted with ethyl alcohol ) to remove insoluble wax and concentrating it under reduced pressure to obtain essential oil from which the solvent has been removed, but is not limited thereto.

In one embodiment of the present invention, the essential oil may be characterized in that it contains a volatile organic compound, but is not limited thereto.

In one embodiment of the present invention, the volatile organic compound is palmitic acid, ethyl palmitate, Eicosane, Heneicosane, linoleic acid, linolenic acid (Linolenic acid), Ethyl linoleate, Ethyl linolenate, Docosane, Tricosane, Tetracosane, Pentacosane, Tetradecane (Tetradecane), Squalene, 1-Nonadecene, δ-Tocopherol, 22,23-Dihydrobrassicasterol, Clionasterol ), β-amyrin (β-Amyrin) and α- amyrin (α-Amyrin) may be characterized in that it includes any one or more selected from the group consisting of, but is not limited thereto.

In one embodiment of the present invention, the essential oil may be characterized in that it is included in the range of 1 to 250 μg / mL, preferably 10 to 150 μg / mL, more preferably 50 to 150 μg / mL It may be 100 μg/mL, but is not limited thereto.

In one embodiment of the present invention, the composition may promote proliferation and migration of epidermal keratinocytes, but is not limited thereto.

In one embodiment of the present invention, the composition may promote the synthesis of collagen type I or collagen type IV of epidermal keratinocytes, but is not limited thereto.

In one embodiment of the present invention, the composition may be characterized in that it induces a growth promoting effect of epidermal keratinocyte sprouting (sprouting), but is not limited thereto.

In addition, the present invention provides a composition for external application for skin for wound treatment comprising, as an active ingredient, an essential oil extracted from Kerria japonica f. pleniflora (Witte) Rehder) flowers.

The present invention identifies the components of essential oil extracted from the flower of Kerria japonica f. pleniflora (Witte) Rehder, which induces proliferation of epidermal keratinocytes and type I and IV collagen synthesis, It induces sprout out growth of the base in vitro artificial skin model in a concentration-dependent manner, induces skin tissue regeneration, and has an effect of improving wrinkles through enhancement of skin elasticity. Therefore, it can be variously applied as a cosmetic composition or an external preparation for the skin.

1 is a flow chart showing a process of extracting essential oil, essential oil or absolute extracted from bamboo flowers in one embodiment of the present invention.
Figure 2 is a diagram showing the toxicity of essential oils extracted from Jukdanhwa flowers to epidermal keratinocytes in one embodiment of the present invention.
3 is a diagram showing the effect of the essential oil extracted from the flower of Jukdanhwa on the proliferation of epidermal keratinocytes in one embodiment of the present invention.
4 is a diagram showing the effect of essential oil extracted from a flower of Jukdanhwa on sprout out growth of an epidermal keratinocyte-based in vitro artificial skin model in one embodiment of the present invention.
Figure 5a is a diagram showing the effect of essential oil extracted from Jukdanhwa flower on type I collagen synthesis in one embodiment of the present invention.
Figure 5b is a diagram showing the effect of essential oil extracted from jukdanhwa flowers on type IV collagen synthesis in one embodiment of the present invention.

Hereinafter, the present invention will be described in detail as an embodiment of the present invention with reference to the accompanying drawings. However, the following implementation examples are presented as examples of the present invention, and if it is determined that a detailed description of a well-known technology or configuration may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. , the present invention is not limited thereby. Various modifications and applications of the present invention are possible within the description of the claims described later and equivalent scopes interpreted therefrom.

In addition, the terminology used in this specification is a term used to appropriately express a preferred embodiment of the present invention, which may vary according to the intention of a user or operator or customs in the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the contents throughout this specification. Throughout the specification, when a certain component is said to "include", it means that it may further include other components without excluding other components unless otherwise stated.

Throughout this specification, '%' used to indicate the concentration of a particular substance is solid/solid (w/w) %, solid/liquid (w/v) %, and Liquid/liquid is (v/v) %.

In one aspect, it relates to a cosmetic composition for skin regeneration or wrinkle improvement comprising essential oil extracted from Kerria japonica f. pleniflora (Witte) Rehder flower as an active ingredient.

In one embodiment, the essential oil extracted from the flowers of Kerria japonica f. pleniflora (Witte) Rehder of the present invention is extracted with hexane (n-hexane) and then concentrated under reduced pressure to form solid concrete. It can be prepared by including the step of obtaining, the step of dissolving the solid concrete in ethyl alcohol to remove insoluble wax, and the step of concentrating it under reduced pressure to obtain essential oil from which the solvent is removed.

In one embodiment, the method for obtaining the concrete and the method for obtaining the essential oil of the present invention may include all methods that can be modified by those skilled in the art.

In one embodiment, the essential oil extracted from the flower of Kerria japonica f. pleniflora (Witte) Rehder of the present invention is palmitic acid, ethyl palmitate, Eicosane, Heneicosane, Linoleic acid, Linolenic acid, Ethyl linoleate, Ethyl linolenate, Docosane, Tricosane, Tetracosane (Tetracosane), Pentacosane, Tetradecane, Squalene, 1-Nonadecene, δ-Tocopherol, 22,23-Dihydrobrasica Contains at least one volatile organic compound component selected from the group consisting of sterol (22,23-Dihydrobrassicasterol), clionasterol, β-Amyrin and α-Amyrin can do.

In one embodiment, the flower of Kerria japonica f. pleniflora (Witte) Rehder) may be a bud just before flowering, and may be a flower that is not dried.

The term “essential oil” used in the present invention is a general term for aromatic oils (volatile substances) extracted and separated from plants (plant leaves, stems, fruits, flowers, roots, etc.). Essential oil derived from natural products is classified into essential oil, absolute, or concrete depending on the extraction method.

As used herein, the term "extract" means an active ingredient isolated from a natural product, that is, a material showing a desired activity. The extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes any form formulated using the dry powder or the extract thereof. In addition, the extract includes a fractionated extract that has undergone the extraction process. The extraction method of the extract is not particularly limited, and may be extracted by, for example, stirring extraction, shaking extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction. As the extraction solvent, a polar solvent such as water or a C ₁ -C ₄ lower alcohol, a non-polar solvent such as hexane, chloroform, dichloromethane or ethyl acetate, or a mixture of two or more thereof may be used.

The cosmetic composition of the present invention may be prepared by including a cosmetically effective amount of the essential oil extracted from the Kerria japonica f. pleniflora (Witte) Rehder flower of the present invention described above and a cosmetically acceptable carrier. there is.

As used herein, the term "cosmetically effective amount" means an amount sufficient to achieve the skin regeneration effect through the proliferation of epidermal keratinocytes of the composition of the present invention described above.

The appearance of the cosmetic composition contains a cosmetic or dermatologically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solids, anhydrous pasty products, emulsions obtained by dispersing an oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) and It may be provided in the form of a non-ionic follicular dispersant, or in the form of a cream, toner, lotion, powder, ointment, spray or conceal stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its dosage form, for example, softening lotion, astringent lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, eye essence, cleansing It can be formulated into cosmetics such as cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fibers, vegetable fibers, wax, paraffin, starch, tracanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. this can be used

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally chlorofluorohydro propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate , fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives, or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention can be applied to skin, lotion, cream, essence, pack, foundation, color cosmetics, sunscreen, two-way cake, face powder, compact, makeup base, skin cover, eye shadow, lipstick, lip gloss, lip fix, eyebrow pencil , It can be applied to cosmetics such as lotion and detergents such as shampoo and soap.

The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the essential oil extracted from the flower of Kerria japonica f. pleniflora (Witte) Rehder. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extracts.

In addition to the above functional additives, the cosmetic composition of the present invention may further contain components included in general cosmetic compositions as needed. Ingredients other than those included include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators, cooling agents, antiperspirants, purified water and the like.

In one aspect, the present invention relates to a composition for external application for skin for wound treatment comprising, as an active ingredient, an essential oil extracted from Kerria japonica f. pleniflora (Witte) Rehder) flowers.

In one embodiment, the composition for external application for skin of the present invention may be used for epidermal keratinocyte proliferation, regeneration, and collagen formation, which is used for skin elasticity, regeneration, and/or Or it may be useful for wound healing.

In one embodiment, the composition for external application for skin of the present invention may use essential oil extracted from Kerria japonica f. It is more preferable to include it at a concentration of because it has the best skin regeneration and wound healing effect.

Advantages and characteristics of the present invention, and methods for achieving them will become clear with reference to examples or experimental examples described later in detail. Hereinafter, the present invention will be described in detail by examples or experimental examples. However, these examples or experimental examples are intended to specifically explain the present invention, and the scope of the present invention is not limited to these examples.

<Example 1> Preparation of bamboo flower essential oil

Referring to the process shown in Figure 1, essential oil (essential oil) was prepared from Jukdanhwa flowers. Jukdanhwa flowers were collected from Haenam, Jeollanam-do (Hanaro Farm), and extracted using an organic solvent extraction method without drying them. Specifically, jukdanhwa flowers were extracted with hexane (n-hexane), and the precipitate thus obtained was evaporated and concentrated by a rotary evaporation to obtain solid concrete. Then, after dissolving the concrete by adding ethyl alcohol, insoluble wax was removed through a vacuum cooling filtration, and the solvent was removed again using a rotary evaporation to finally Jukdanhwa flower essential oil was prepared. Then, the prepared jukdanhwa flower essential oil was stored at a low temperature of -80 ℃ until used in the experiment.

<Example 2> Component Analysis of Jukdanhwa Flower Essential Oil

Component analysis of the Jukdanhwa flower essential oil prepared in Example 1 was requested to KBSI (Korea Basic Science Institute, Seoul Center). For analysis, a gas chromatography/mass spectrometer (6890N GC/5975i MS, Agilent, USA) was used, and the column of GC/MS (Gas chromatography-mass spectrometry) was DB5-MS (30 m × 50 μm, 0.25 μm) was used, and the carrier gas was analyzed by flowing at 1 mL/min using helium. The temperature of the oven was maintained at 40 ° C - 2 ° C / min - 230 ° C - 5 ° C / min - 300 ° C (5 minutes), and the temperature of the injection port was analyzed at 280 ° C. In addition, the temperature of the interface was analyzed at 300 ° C, the temperature of the ion source was 230 ° C, and the temperature of the analyzer was 150 ° C, and the mass range was from 40 to 800 m / z. analyzed. Identification of the essential oil components of Jukdanhwa flower was confirmed by matching the retention indices (RI) of the volatile component with the retention time of the standard substance or by comparing the mass spectrum of the volatile substance with the mass spectrum of the Wiley 7Nist 05 library. RI was determined under the same conditions as the sample using C6 to C24 (n-alkane).

As a result, it was confirmed that it is composed of a total of 20 components as shown in Table 1 below. The main components are Tricosane 34.21%, α-Amyrin 14.97%, Pentacosane 9.45%, Squalene 6.30%, 1-Nonadecene 4.25% , Palmitic acid 3.63%, β-Amyrin 3.31%, Clionasterol 3.26%, Tetracosane 3.24%, Heneicosane 3.13%, Docosane 2.95%, Ethyl linolenate 2.07%, δ-Tocopherol 1.86%, Linolenic acid 1.75%, Linoleic acid 1.72%, Ethyl It was confirmed that linoleate 1.12%, Tetradecane 0.96%, Eicosane 0.67%, Ethyl palmitate 0.59%, and 22,23-Dihydrobrassicasterol 0.56% in order of high content (Table 1).

<Example 3> Measurement of the effect of Jukdanhwa flower essential oil on the survival rate of keratinocytes

Human skin-derived epidermal keratinocytes (HaCat) were cultured using DMEM (Dulbeco's Modified Eagle's Medium) medium containing 10% fetal bovine serum and used in the present invention. Epidermal keratinocytes were injected into a 96 well microtiter plate at a concentration of 5×10 ³ cells/100 μL/well per well, and adhered in a 37° C., 5% CO ₂ incubator. 100 μL of the Jukdan flower essential oil prepared in Example 1 was cultured in each well at a concentration of 1 to 250 μg / mL, and as a negative control group, the Jukdan flower essential oil was untreated and cultured. After 24 hours, Ez-cytox was treated in each well and reacted for 30 minutes in an incubator. After measuring the absorbance at 450 nm using an ELISA plate reader (Synerge2, BioTek, USA), converting it into a percentage, the survival rate result for epidermal keratinocytes of Jukdanhwa flower essential oil was secured.

As a result, as shown in FIG. 2, no significant cytotoxicity was observed at concentrations of 1 to 150 μg/mL of Jukdanhwa flower essential oil. However, it was confirmed to have cytotoxicity at a concentration of 250 μg/mL. Therefore, it was confirmed that the concentration of Jukdanhwa flower essential oil according to the present invention is preferably 150 μg / mL or less, and it was applied to subsequent experiments.

<Example 4> Effect of Jukdanhwa flower essential oil on proliferation of epidermal keratinocytes

Human skin-derived epidermal keratinocytes (HaCaT) were cultured using DMEM medium containing 10% fetal bovine serum, and used in the present invention. Epidermal keratinocytes were injected into a 96 well microtiter plate at a concentration of 3×10 ³ cells/100 μL/well per well, and adhered in a 37° C., 5% CO ₂ incubator. Jukdanhwa flower essential oil was cultured by treating 100 μL in each well at a concentration of 0.1 to 100 μg/mL, and the negative control group was untreated with Jukdanhwa flower essential oil, and the positive control group was conventionally used for wound healing or skin regeneration. Epidermal growth factor (EGF, 50 ng/mL) was treated and cultured for 48 hours. Ez-cytox was treated in each well and reacted for 30 minutes in an incubator. After measuring the absorbance at 450 nm using an ELISA plate reader (Synerge2, BioTek, USA), it was converted into a percentage.

As a result, as shown in FIG. 3, it was found that Jukdanhwa flower essential oil induces the proliferation of epidermal keratinocytes in a concentration-dependent manner. The proliferation of epidermal keratinocytes by the positive control group was induced by 159.85±4.79%, and the proliferation induction rate of 122.44±5.00% was shown at the concentration of 10 μg/mL of Jukdanhwa flower essential oil, and at the concentration of 50 μg/mL, 223.37 It showed the maximum proliferation induction rate of ±3.74%.

<Example 5> Effect of Jukdanhwa flower essential oil on sprout out growth of epidermal keratinocyte-based in vitro artificial skin model

After mixing Type I collagen and epidermal keratinocytes to 2.5×10 ⁷ cells/mL, they were spotted on a 24 well plate and dried to form an artificial skin tissue. Jukdanhwa flower essential oil was treated by concentration, the negative control group did not use Jukdanhwa flower essential oil, and the positive control group was epidermal growth factor (EGF, 50), which is used for conventional wound healing or skin regeneration. ng/mL) was used. Negative or positive control was treated, and cultured in a 37°C, 5% CO ₂ incubator for 48 hours. The culture medium was removed, cells and tissues were fixed and stained using Diff-Quik solution, and the results were obtained by photographing the stained cells and tissues.

As a result, FIG. 4 shows that the essential oil of the flower extract extracted in Example 1 of the present invention induces sprout out growth of the epidermal keratinocyte-based in vitro artificial skin model in a concentration-dependent manner. The positive control group induced 705.92±42.71% out growth, and the Jukdanhwa flower essential oil showed the maximum outgrowth induction rate of 609.27±31.26% at a concentration of 50 μg/mL.

<Example 6> Effect of Jukdanhwa Flower Essential Oil on Type I or IV Collagen Synthesis and Secretion of Epidermal Keratinocytes

A 96 well microtiter plate was coated with monoclonal anti-collagen I or IV for 12 hours after injection. After blocking, each sample was reacted by treating 100 μL per well. The sample was treated with the cell culture medium of the jukdanhwa flower essential oil treatment group or the untreated group. Thereafter, polyclonal anti-collagen I or IV was treated in each well and reacted for 90 minutes, and then streptavidin-POD was treated in each well and reacted for 60 minutes. After injecting the ELISA pico chemiluminescent substrate into each well, the luminescence was measured using an ELISA plate reader, and the results of type I or IV collagen synthesis and secretion of epidermal keratinocytes of Jukdanhwa flower essential oil were obtained.

As a result, as shown in FIG. 5, the essential oil of the flower of Jukdanhwa significantly promoted the synthesis and secretion of type I and IV collagen of epidermal keratinocytes at concentrations of 50 μg/mL and 100 μg/mL. In particular, it was confirmed that the synthesis of type I (160.24±12.45%) and IV (225.07±18.35%) was induced to the maximum at the concentration of 100 μg/mL of essential oil of Jukdanhwa flower.

So far, the present invention has been looked at mainly with its preferred embodiments. Those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from a descriptive point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent scope will be construed as being included in the present invention.

Claims (10)

A cosmetic composition for skin regeneration or wrinkle improvement comprising essential oil extracted from Kerria japonica f. pleniflora (Witte) Rehder as an active ingredient. According to claim 1,
The essential oil is a cosmetic composition for skin regeneration or wrinkle improvement that is extracted from bamboo flowers. According to claim 1,
The essential oil is obtained by extracting bamboo flowers with n-hexane and then concentrating under reduced pressure to obtain solid concrete, and dissolving the solid concrete in ethyl alcohol to remove insoluble wax A cosmetic composition for skin regeneration or wrinkle improvement, which is prepared by including the steps of obtaining an essential oil from which the solvent is removed by concentrating it under reduced pressure. According to claim 1,
The essential oil is a cosmetic composition for skin regeneration or wrinkle improvement comprising a volatile organic compound. According to claim 4,
The volatile organic compound is palmitic acid, ethyl palmitate, Eicosane, Heneicosane, linoleic acid, linolenic acid, ethyl linoleate ( Ethyl linoleate), Ethyl linolenate, Docosane, Tricosane, Tetracosane, Pentacosane, Tetradecane, Squalene, 1-Nonadecene, δ-Tocopherol, 22,23-Dihydrobrassicasterol, Clionasterol, β-Amyrin (β- Amyrin) and α- amyrin (α-Amyrin) that contains any one or more selected from the group consisting of, a cosmetic composition for skin regeneration or wrinkle improvement. According to claim 1,
The essential oil is contained in the range of 1 to 250 μg / mL, a cosmetic composition for skin regeneration or wrinkle improvement. According to claim 1,
The composition promotes the proliferation and migration of epidermal keratinocytes, a cosmetic composition for skin regeneration or wrinkle improvement. According to claim 1,
The composition promotes the synthesis of collagen type I or collagen type IV of epidermal keratinocytes, a cosmetic composition for skin regeneration or wrinkle improvement. According to claim 1,
The composition induces a growth promoting effect of epidermal keratinocyte sprouts (sprouting), a cosmetic composition for skin regeneration or wrinkle improvement. A composition for external application for skin for wound treatment comprising essential oil extracted from Kerria japonica f. pleniflora (Witte) Rehder flower as an active ingredient.

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