KR20180051821A - Composition for skin regeneration containing extracted from erigeron annuus (l.) pers flower - Google Patents

Abstract

The present invention relates to a skin regeneration composition comprising extracted essential oil extracted from an Erigeron annuus (L.) Pers. flower, and to a cosmetic material. The essential oil induces proliferation and migration of epidermal keratinocytes, thereby providing an excellent skin regeneration effect. The essential oil is extracted by steam distillation. The essential oil includes at least one component selected from the group consisting of myrcene, cis-β-ocimene, β-elemene, β-caryophyllene, E-β-farnesene, germacrene D, benzene, and 1-(-1-cycolopropylethenyl)-4-methoxy)-.

Description

TECHNICAL FIELD The present invention relates to a composition for skin regeneration comprising essential oil extracted from an apterous flower. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for skin regeneration,

The present invention relates to a composition for skin regeneration, a cosmetic composition, and a composition for external application for skin, which comprises an essential oil extracted from Erigeron annuus (L.) Pers flower as an active ingredient.

The skin is located at the outermost part of the human body and directly touches the external environment, protecting the human body from external damage, and preventing excessive evaporation of moisture and heat in the body. The skin consists of three layers of epidermis, dermis and subcutaneous tissue, and most of the outermost epidermis consists of keratinocytes. The keratinocytes of the epidermis form proliferation, migration and differentiation from the basal layer to form keratin to protect the skin and produce various cytokines, which are involved in the inflammatory and immune responses. In addition, proliferation and migration of keratinocyte cells not only provide wound healing and skin regeneration but also induce collagen synthesis, thereby enhancing skin elasticity and basement membrane formation, thereby controlling adhesion and proliferation of skin cells, Enhancement of the barrier function to selectively transmit the substance and interaction of the epidermis with the dermis. As a result, migration and proliferation of skin epidermal keratinocytes promote skin renewal and wound healing as well as skin elasticity and maintain healthy skin.

On the other hand, Erigeron annuus (L.) Pers.) Is a biennial plant belonging to Asteraceae. It is native to North America and distributed worldwide. In Korea, Erigeron annuus (L.) Pers. It is a plant. It grows up to 30-100 cm in height and branches off from the upper part, and has a thick hairs throughout. The flower blooms in June-July, running on the tip of the branch and the end of the main stem, white with a diameter of about 2 cm, contains a large amount of aromatic compounds and has a unique scent. In the private sector, it has been known to have medicinal effects such as gastroenteritis, indigestion, gastroenteritis due to gastroenteritis, diarrhea, hypoglycemic effect, hematuria, malaria, and anti-inflammation.

However, it is still widely used in the fields of Korea, and it can be easily harvested and harvested in large quantities. It has a unique flavor and is highly industrially useful value. However, it is very useful as α-amyenone, α-amyrin and β-amyrin In addition to pharmacological and physiological studies, the study of essential oil components contained in apricot flowers has been lacking.

Essential oil derived from natural products is a volatile chemical substance that shows pharmacological effects on the human body among the secondary metabolites of plants, and is composed of various organic compounds such as alcohols, aldehydes, esters and ketones. The unique fragrance ingredients and therapeutic ingredients of essential oils are effective in relieving stress through the skin, circulatory system and posterior circulation, recovering defenses and relaxing muscles, as well as antimicrobial action, immunity enhancement and antiallergic action. And is effective in beauty such as prevention of aging. This effect of essential oils continues to be modern, and various studies are required not only for pharmaceuticals and foods but also for cosmetics.

Korean Patent Publication No. 2008-0023827

In order to solve the above problems, the present invention has been completed by confirming the components of the essential oil extracted from Angelica keiskei koidz., Confirming its skin elasticity, skin regeneration and wound healing efficacy.

In order to achieve the above object, the present invention provides a skin regeneration composition comprising an essential oil extracted from Erigeron annuus (L.) Pers flower as an active ingredient.

In one embodiment of the present invention, the essential oil may be extracted by steam distillation and may be prepared by removing the aqueous solution component from the distillate extracted by steam distillation.

In one embodiment of the present invention, the reptiles may be non-dried.

In one embodiment of the present invention, the essential oil is selected from the group consisting of Myrcene, cis-β-Ocimene, β-Elemene, β-Caryophyllene, E- (1-cyclohexylmethyl) benzene, β-Farnesene, Germacrene D, Benzene, 1 - (1-cycolopropylethenyl) -4-methoxy) -.

In one embodiment of the present invention, the essential oil may be contained in the range of 0.0001 to 10 μg / ml, for example, in the range of 0.1 to 1 μg / ml.

In one embodiment of the present invention, the essential oil may induce proliferation and migration of epidermal keratinocytes. Specifically, the essential oil may induce one or more phosphorylation or signal transduction processes selected from the group consisting of epinephrine keratinocyte serine / threonine kinase (Akt) and extracellular signal regulated kinase (ERK1 / 2).

Further, the present invention provides a cosmetic composition comprising an essential oil extracted from Angelica keiskei koidz. As an active ingredient.

In addition, the present invention provides an external composition for skin comprising an essential oil extracted from Angelica keiskei koidz. As an active ingredient.

The present invention discloses an essential oil component extracted from an apterous flower, demonstrates skin elasticity improvement, skin regeneration and wound healing efficacy, and exhibits activity of inducing proliferation and / or migration of epidermal keratinocytes, / Threonine kinase (Akt) and extracellular signal-regulated kinase (ERK1 / 2) to regulate cell proliferation and thus have excellent effects on skin regeneration. In addition, the essential oil extracted from Angelica keiskei according to the present invention exhibits an improvement in skin elasticity and a wound healing effect, and can be applied variously as a cosmetic preparation, an external preparation for skin and the like.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart illustrating a process for extracting essential oils from an amphipathic flower in an embodiment of the present invention. FIG.
FIG. 2 is a graph showing the result of analysis of the essential oil components from the amphipods in an embodiment of the present invention. FIG.
FIG. 3 is a graph showing an effect of essential oil of migratory flower on migration of keratinocytes in an embodiment of the present invention.
FIG. 4 is a graph showing the effect of essential oil of the immature flower of the present invention on proliferation of keratinocytes in an embodiment of the present invention.
FIG. 5 is a graph showing the effect of the essential oil (A) on the phosphorylation of the epidermal keratinocyte subcellular / threonine protein kinase (Akt) and the extracellular signal regulated kinase (ERK1 / 2) (B) a graph depicting the results on the phosphorylation of serine / threonine protein kinase (Akt) in epidermal keratinocyte, and (C) a graph showing the phosphorylation of epidermal keratinocyte (ERK1 / 2) And the results are quantified.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, detailed description of known techniques well known to those skilled in the art may be omitted. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. Also, terminologies used herein are terms used to properly represent preferred embodiments of the present invention, which may vary depending on the user, intent of the operator, or custom in the field to which the present invention belongs.

Therefore, the definition of these terms should be based on the contents throughout this specification. Throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements as well, without excluding other elements unless specifically stated otherwise.

The present invention relates to a skin regeneration composition, a cosmetic composition and a composition for external application for skin, which comprises an essential oil extracted from Erigeron annuus (L.) Persia flower as an active ingredient

The above-mentioned essential oils are preferably obtained from non-dried apricot flowers, and can be prepared according to a steam distillation process. The steam distillation method can be produced in a manner commonly used in the art, for example, by a steam distillation method or a steam distillation-extraction method simultaneously. The water vapor distillation method can be used to produce anchovy essential oil by distillation using a steam distillation method after washing the anchovy according to the process shown in FIG. 1, cooling it at a low temperature, separating and purifying it, .

Specifically, the essential oil extracted from the Japanese apricot flowers of the present invention is washed and dried, followed by pulverization. The pulverized product is treated with an anhydrous or hydrous alcohol having 1 to 4 carbon atoms, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol , Ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform, and hexane, followed by cooling or warming, followed by extraction at least once. For example, a first extraction using one or more of an anhydrous and a lower alcohol having 1 to 4 carbon atoms, a polar solvent containing acetone and butylene glycol, and then successively extraction with ethyl acetate, diethyl acetate, diethyl ether , And a low-polarity solvent including benzene, chloroform, and hexane. Further, the extract obtained in the above may be further concentrated in a distillation apparatus to a suitable temperature and concentrated.

The extraction method may be carried out for about 12 to 96 hours in the case of cold beating, or by aging at a temperature of 4 to 25 DEG C for 1 to 20 days in an anhydrous or hydrolyzed alcohol having 1 to 4 carbon atoms, ethyl acetate or diethyl ether as a solvent, The active ingredient can be extracted. In the case of warming, it is preferable that the heating is carried out at a temperature close to the reflux temperature of the solvent for about 5 to 24 hours although it depends on the kind and temperature of the extraction solvent.

The essential oil extracted from the petioles according to the present invention contains various physiologically active ingredients, and more specifically, it contains myrcene, cis-β-Ocimene, β-Elemene, At least one component selected from the group consisting of β-Caryophyllene, E-β-Farnesene, Germacrene D, Benzene and 1- (-1-cycolopropylethenyl) -4-methoxy - But it is not limited thereto (Fig. 2).

The composition of the present invention is not particularly limited and may be contained in an amount within the range of 0.0001 to 10 占 퐂 / ml, preferably 0.1 to 1 占 퐂 / ml, . That is, the skin epidermal keratinocytes showed a high cell survival rate at a concentration within the range of 0.0001 ~ 10 / / ㎖ of the above-mentioned apricot essential oil, and it was found to have high safety without any danger such as skin disease or side effects due to the apricot flower essential oil But is cell-friendly, and is preferable as a raw material for cosmetics or medicines for skin elasticity enhancement and regeneration and wound healing, and external preparation for skin.

In addition, it is possible to induce the proliferation and / or migration of epidermal keratinocytes. In other words, as can be seen from the examples and experiments to be described later, the migratory flower essential oil according to the present invention induces the migration of epidermal keratinocytes in a concentration-dependent manner. In particular, at the concentration of 0.1, 1 ㎍ / Thereby effectively inducing cell migration (Experimental Example 1 and Fig. 3). In addition, the growth of epidermal keratinocytes was induced in a concentration-dependent manner, and the proliferation of epidermal keratinocytes was induced at a concentration of 0.01, 0.1, and 1 / / ㎖, respectively Example 2 and Fig. 4). In addition, the apricot essential oil may induce at least one phosphorylation process or signal transduction process selected from the group consisting of epinephrine keratinocyte serine / threonine kinase (Akt) and extracellular signal regulated kinase (ERK1 / 2) . That is, as can be seen from the examples and experiments to be described later, the angora essential oil according to the present invention is useful for the phosphorylation of epinephrine keratinocyte serine / threonine kinase (Akt) and extracellular signal regulated kinase (ERK1 / 2) Induced phosphorylation of serine / threonine kinase (Akt) and extracellular signal regulated kinase (ERK1 / 2) at a concentration of 1 / / ml (see Experimental Example 3 and FIG. 5).

As described above, according to the present invention, it is possible to obtain the skin elasticity and the skin elasticity of the epidermal keratinocyte, which is obtained by the steam distillation method against the amphipods, by the skin aging, the wrinkles, , Regeneration, and / or wound healing.

Accordingly, the present invention may be a composition for skin regeneration, a composition for external application for skin, or a cosmetic composition, which comprises an essential oil of Angora extracted from an amphipathic flower as an active ingredient. Such a composition includes both medicines and quasi-drugs, and can be variously used for skin elasticity enhancement, skin regeneration, and / or skin wound healing.

The composition for skin regeneration, which comprises an essential oil of Angora extract extracted from an amphipathic flower of the present invention as an active ingredient, can be used as a pharmaceutical composition and contains a pharmaceutically effective amount of an apterous flower essential oil alone Or one or more pharmaceutically acceptable carriers, excipients or diluents.

The "pharmaceutically effective amount" as used herein refers to an amount sufficient for the physiologically active component to be administered to an animal or a human to exhibit a desired physiological or pharmacological activity. However, the pharmaceutically effective amount may be appropriately changed depending on the severity of symptoms, the age, body weight, health condition, sex, administration route and treatment period of the patient.

The term "pharmaceutically acceptable" as used herein means physiologically acceptable and does not generally cause an allergic reaction such as gastrointestinal disorder, dizziness, or the like when administered to a human. Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. Further, it may further include a filler, an anticoagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent and an antiseptic agent.

The compositions of the present invention may also be formulated using methods known in the art so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal, and may be formulated as a variety of compositions for oral or parenteral administration . ≪ / RTI > The formulations may be in the form of powders, granules, tablets, emulsions, syrups, aerosols, soft or hard gelatine capsules, sterile injectable solutions, sterile powders, ointments, lotions, creams,

The composition according to the present invention may be administered through various routes including oral, transdermal, subcutaneous, intravenous or muscular, and the dose of the active ingredient may be varied depending on various factors such as route of administration, age, sex, And the like. In addition, the composition of the present invention may be administered in combination with a known compound capable of raising the desired effect.

Furthermore, the composition according to the present invention can be used not only as a pharmaceutical composition as described above, but also as a health functional food. For example, it can be easily used as a main ingredient, additives, food additives, functional foods or beverages of food for the purpose of skin elasticity and regeneration effect.

The term " food " means a natural product or a processed product containing one or more nutrients, preferably a state of being able to be eaten directly through a certain degree of processing, , Food additives, functional foods and beverages.

Foods to which the food composition can be added include, for example, various foods, beverages, gums, tea, vitamin complexes, and functional foods. In addition, it is also possible to use special nutritive foods (eg crude oil, milk, infant food, etc.), meat products, fish products, tofu, jelly, noodles (eg ramen, (Such as fermented milk, cheese, etc.), processed foods, kimchi, pickled foods (various kinds of kimchi, etc.), sauces, confectionery (eg, snacks), candies, chocolate, gums, ice cream, But not limited to, beverages (e.g., fruit drinks, vegetable beverages, beverages, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.). The food, beverage or food additive may be prepared by a conventional production method.

The term " functional food " or " health functional food " refers to a food group imparted with added value to function or express the function of the food to a specific purpose by physical, biochemical or biotechnological techniques, Defensive rhythm control, prevention and restoration of disease, and the like, and it can be a health functional food. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.

The health supplements may be in the form of powders, granules, tablets, capsules or drinks, although not limited thereto.

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are for illustrating the present invention specifically, and the scope of the present invention is not limited to these examples.

[ Example ]

Example  1: Essential  Manufacture of oil

According to the process shown in Fig. 1, Angora essential oil was prepared.

Amanthoptera was harvested from June to July in Hosseo University (Asan, Korea). 18.6 kg of raw vertebra was obtained by steam distillation to obtain about 12 L of apricot distillate, cooled at a low temperature, separated and purified, and the aqueous solution component To obtain 20 mL of essential oil. Then, the obtained amber flower essential oil was stored at a low temperature of 4 캜.

Example  2. Angora flower Essential  Analysis of components of oil

The composition analysis of the petiole oil essential oil prepared in Example 1 was commissioned by KBSI (korea basic science institute, seoul center). The GC / MS column was DB5-MS (30 m x 250 m, 0.25 m), and the carrier gas (" carrier gas) was analyzed by flowing it at 1 mL / min using helium. The temperature of the oven was maintained at 40 ° C - 2 ° C / min - 230 ° C - 5 ° C / min - 300 ° C (5 minutes) and the injection temperature was analyzed at 280 ° C. The temperature of the interface was 300 ℃, the temperature of the ion source was 230 ℃, the temperature of the analyzer was 150 ℃ and the mass range was 40-800 m / z.

Identification of the essential oil component of the petiole was confirmed by comparing the retention indices (RI) of the volatile components with the lag time of the reference material or by comparing the mass spectrum of the volatiles with the mass spectra of Wiley 7Nist 05 library. RI was determined under the same conditions as the samples using C6-C24 (nalkane). As a result, it was confirmed that it consisted of 7 components in total, and showed higher contents in the order of Germacrene D, Benzene, 1- (1-cyclopropylethenyl) -4-methoxy- and β-Elemene (Fig. 2).

Experimental Example  1. Angora flower Essential  Effect of oil on keratinocyte migration

In order to confirm the effect on the epidermal keratinocyte migration using the apricot flower essential oil obtained in Example 1, the following experiment was conducted.

Human skin-derived epidermal keratinocytes (HaCat) were cultured in DMEM medium containing 10% fetal bovine serum. The lower chamber was treated with 0.0001 ~ 10 μg / mL apricot essential oil at a concentration of 0.0001 ~ 10 μg / mL in a 48 well Boyden Microchemotaxis Chamber. The poly-carbonate membrane . Then, keratinocytes were treated with 1 × 10 ⁶ cells / mL per well in the upper chamber. The chamber was incubated at 37 ° C in a 5% CO ₂ incubator for 3 hours, and the membrane was fixed and stained with Diff-Quik solution. The number of cells migrated through the membrane was counted and converted to a percentage to obtain the results of skin epidermal keratinocyte migration of the apricot essential oil. The negative control group did not use the apricot essential oil, and the positive control group was the epithermal growth factor (EGF 5 ng / mL) used for conventional wound healing and / or skin regeneration.

As a result, as shown in FIG. 3, the migration effect of keratinocyte cells of anchovy essential oil obtained by the examples of the present invention was shown, and it was 334.4 ± 13.80% at a concentration of 0.1 μg / mL of anchovy essential oil And showed the maximum migration rate of 363.1 ± 14.80% at 1 μg / mL concentration, indicating that the apricot flower essential oil effectively induced migration of keratinocytes.

Experimental Example  2. Angora flower Essential  Effect of oil on keratinocyte proliferation

In order to confirm the effect on the epidermal keratinocyte proliferation using the apricot flower essential oil obtained in Example 1, the following experiment was conducted.

Human skin-derived epidermal keratinocytes (HaCat) were cultured in DMEM medium containing 10% fetal bovine serum. Epidermal keratinocytes were injected into 96-well microtiter plates at a density of 2 × 10 ³ cells / 100 μL / well per well and adhered in a 37, 5% CO ₂ incubator. 100 μL of each of the wells was treated with 0.0001 ~ 10 μg / mL of the apricot essential oil and cultured for 48 hours.

EZ-cytox and DEME were mixed at a ratio of 1: 1, treated with 20 μL of each well, and reacted for 30 minutes in an incubator. The absorbance at 450 nm was measured using an ELISA plate reader (Synerge2, BioTek, USA), and the results were obtained as percentage of skin epidermal keratinocyte proliferation of the apricot essential oil. The negative control group did not use the apricot essential oil, and the positive control group used epithermal growth factor (EGF 50 ng / mL), which is used for conventional wound healing and / or skin regeneration.

As a result, as shown in Fig. 4, the apricot essential oil showed concentration-dependent induction of epidermal keratinocyte proliferation. In particular, it showed 126.5 ± 4.998% proliferation induction rate at the concentration of 0.1 μg / mL of the apricot essential oil and 153.1 ± 2.967% at the concentration of 1 μg / mL. Proliferation is effectively induced.

Experimental Example  3. Angora flower Essential  Oil Within keratinocyte  Effect on Signal Transduction

In the phosphorylation process of serine / threonine kinase (Akt) and extracellular signal-regulated kinase (ERK1 / 2), which are the main signal transduction processes that regulate the proliferation and migration of epidermal keratinocytes using the petiole oil essential oil obtained in Example 1 (PAkt, Akt, P-ERK1 / 2, and ERK1 / 2) were used to measure the effect of the immune-blocking essential oil. The negative control group did not use the apricot essential oil, and the positive control group used epithermal growth factor (EGF 50 ng / mL), which is used for conventional wound healing and / or skin regeneration.

As a result, as shown in FIG. 5, the apricot essential oil induced phosphorylation of epinephrine keratinocyte serine / threonine kinase (Akt) and extracellular signal regulated kinase (ERK1 / 2) / mL, induction of intracellular serine threonine kinase (Akt) phosphorylation was 229.5 ± 4.522%, and that of extracellular signal regulated kinase (ERK1 / 2) was 335.6 ± 16.90%.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

A composition for regenerating skin comprising an essential oil extracted from Erigeron annuus (L.) Pers flower as an active ingredient. The method according to claim 1,
Wherein the essential oil is extracted by a steam distillation method. The method according to claim 1,
The essential oils are selected from the group consisting of Myrcene, cis-β-Ocimene, β-Elemene, β-Caryophyllene, E-β-Farnesene, Wherein the composition comprises at least one member selected from the group consisting of Germacrene D, Benzene, 1 - (- 1-cycolopropylethenyl) -4-methoxy) -. 4. The method according to any one of claims 1 to 3,
Wherein the essential oil is contained in a range of 0.0001 to 10 μg / mL. 5. The method of claim 4,
Wherein the essential oil is contained in a range of 0.1 to 1 μg / mL. 4. The method according to any one of claims 1 to 3,
Wherein the essential oil induces proliferation and migration of epidermal keratinocytes. The method according to claim 6,
Wherein the essential oil induces one or more phosphorylation or signal transduction processes selected from the group consisting of epinephrine keratinocyte serine / threonine kinase (Akt) and extracellular signal regulated kinase (ERK1 / 2). A cosmetic composition comprising an essential oil extracted from an apterous flower as an active ingredient. A composition for external application for skin, comprising essential oil extracted from an amphipathic flower as an active ingredient.

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