KR20230059308A - Hypoallergenic oral perfume composition based on seaweed complex and seawater - Google Patents

Abstract

The present invention relates to a hypoallergenic oral perfume composition made with a seaweed complex and seawater as main ingredients, wherein the composition is made by a method comprising: a natural material preparation step; a preprocessing step; a step for preparing a hot water extract; and a preprocessing step for the preparation of the complex and removal of fishy smell of the seaweed.

Description

Hypoallergenic oral perfume composition based on seaweed complex and seawater}

The present invention relates to a hypoallergenic oral perfume composition using marine materials or seaweed and seawater.

Oral disease status: Oral disease is a chronic disease that affects about 90% of the elderly population over 60 years of age. Oral-related diseases are also diversifying and emerging as one of the serious social problems. 60% of people suffer from dental caries and periodontal disease.

Currently, hypoallergenic gum care oral health products for the senior generation are limited to gum disease treatments based on vegetable herbal extracts and products to which Zefiacorbic acid, tocopherol acetate, carbazochrome, and lysozyme hydrochloride are added. Oral care products related to periodontal disease, including , are mixed with anti-inflammatory substances, and development of a fundamental treatment to prevent the destruction of gums and alveolar bone is insufficient. It can be used as a treatment for gum disease by utilizing components of alginic acid, fucoidan, and laminarin extracted from brown algae. Research on whether to do so is in progress.

Current status of oral care products: Chlorhexidine, the main component of oral sprays, attacks bacterial cell membranes, causing leakage or precipitation of cell components, and also binds to salivary mucin to reduce salivary pellicle formation, thereby interfering with plaque colonization. It is effective in controlling oral harmful bacteria by binding to bacteria and preventing bacteria from adsorbing to the tooth surface. However, chlorhexidine has side effects such as bitter taste, discoloration of the tongue and teeth, and burning sensation of mucous membranes. In addition, the problem of excessive use of these chemicals causes confusion not only in the microflora in the oral cavity but also in the intestinal microflora, leading to side effects such as infection by other bacteria, vomiting and diarrhea, causing distrust among consumers.

Corona and intensifying bad breath: Interest in personal hygiene management and self-care has increased due to the 'post-corona', and in particular, consumers of all ages have increased interest in oral care and bad breath management along with health-oriented. In fact, it can be seen that sales of mouthwash (halitosis remover, chewing gum, candy, etc.) have increased explosively since the outbreak of Corona. In addition, since the mask worn at all times after Corona reduces the use of muscles around the mouth, inhibits self-purification in the mouth and reduces saliva secretion, the bacterial index in the mouth inevitably increases, and along with this, a large amount of harmful gases that cause bad breath It is happening.

Demand for oral care products continues to increase as the wearing time of masks increases after 'Post Corona', and since masks are a part of daily life, there is a desire and tendency to solve them in an efficient way in a short time, and there is a tendency to be sensitive to disgusting odors from the mouth. In the case of millennials and generation Z, interest in bad breath management is rapidly increasing, and they tend to pursue convenient oral care products as they are the convenience-oriented generation.

Overall efficacy of seaweeds: Seaweeds have unique components and functions not found in terrestrial plants, but the technology to extract and utilize them has not been developed, so the use of high value-added products is insignificant. and development of functional research technologies such as improvement of cirrhosis, sarcopenia suppression, and immunity improvement/ Development of various high-tech extraction processes for useful substances derived from seaweeds, discovery of more diverse functional materials derived from seaweeds, and high added value of raw materials are absolutely required./ Seaweed products are currently the largest consumption market among marine organisms in Europe, where seaweed materials have been mainly used as raw materials for pharmaceutical and hormone preparation industries.

○ Seaweeds, unlike land plants, are rich in functional polysaccharides such as fucoidan and alginic acid and unique physiologically active substances, so they have high potential value as future marine health care materials. There is a need to develop various high value-added food and medicine materials such as general food, individually approved health functional food, natural product new drug, functional cosmetics, and functional feed.

On the other hand, seaweed is a fungus that is exposed to strong ultraviolet rays and contains a large amount of components with high antioxidant and anti-inflammatory activities such as polyphenols, so it can withstand such harsh environments. Therefore, seaweeds containing a large amount of components having antioxidant and anti-inflammatory activities are highly likely to induce a high inhibitory effect on metabolic diseases in the human body, and such effective seaweed extracts are highly likely to be applied to the food and drug industry.

○ Functional food field: In the field of functional health functional food, synergistic effects with notified raw materials are created by utilizing seaweed raw materials that have not been used as functional food raw materials to date in accordance with the limitations of developing health functional foods using existing notified raw materials in the field of functional health functional foods. Possibility of new product launch and commercialization through

○ Cosmetics field: Acting as new vitality in areas where market expansion is limited due to the limitations of existing functional cosmetic raw materials, it is possible to launch new products by applying mutual synergistic effects with announced functional cosmetic raw materials and material marketing emphasizing the clean image of the ocean.

○ Functional material field: Possibility to enter the pet field and livestock feed field in addition to the food field and cosmetics field through the development of food preservatives and antibacterial active materials / The unique characteristics of seaweed raw materials (flavor, texture, Applicability as an industrial product reflecting off-flavors, etc.) / Creation of a new industry applied as a material applicable to architecture and art activities in addition to the biological and bio industries

○ The expansion of the seaweed market is attributed to the increase in global demand for natural or non-animal food ingredients with relatively few allergens.

○ Seaweed is evaluated as a healthy food ingredient that can replace eggs or dairy fat in the food sector such as bakery, beverage, and dessert (KMI Monthly Trend, June issue, 2017 Korea Maritime Institute)

○ In addition, seaweed's main useful ingredients, such as omega-3 fatty acids, carrageenan, alginate, agar, and beta-carotene, are highly marketable, and the seaweed market is expected to expand due to increased demand for functional foods and pharmaceutical materials in the future.

○ Analyzing the seaweed industry in Europe, the industries processing carotenoids, cosmetics, fertilizers, etc., and the food and feed industries are the largest, followed by the production of agar, alginic acid, and carrageenan, and aquaculture.

Seaweed-derived physiologically active substances

type bioactive substance uses Raw material polysaccharide carrageenan Gel formation in the meat processing industry macroalgae agar
(Individually approved functional raw materials) gel formation, etc. red algae fucan/fucoidan Functional Food Supplements cell membrane of brown algae fatty acid omega 3 fatty acids functional food most marine life phenolic
compound,
etc
pigment prolotanin Functional food, active additives Brown algae (shellfish, etc.) carotenoids;
beta carotene, lutein Natural pigment, functional enhancer, color for farmed salmon Seaweeds (Dunalielasalina, Sarcinamaxima, Chlorelaprotothecoides, Chlorela vulgaris and Haematococus pluvialis) chlorophyll natural pigment green algae vitamin,
mineral Fat-soluble and water-soluble vitamins, iron, iodine, manganese dioxide, zinc For manufacturing food, health functional food and medicine most seaweed marine enzymes Protein hydrolysis and food processing, enzyme synthesis, fibrinolysis etc Seenol/Cypolynol Ecklonia cava extract/ Ecklonia cava extract antioxidant Ecklonia-Individually approved functional ingredient Complex extracts such as seaweed (Xanthigen) Individually approved functional raw materials Seaweed

Necessity of research: It is judged that product development by domestic and foreign manufacturers will become more active in order to meet the above social changes and consumer demands, and it is predicted that the demand for related products will further expand. In particular, consumers such as civil servants, bank tellers, taxi drivers, courier drivers, A/S drivers, and service workers who directly face customers need small-sized and easy-to-carry bad breath care products, and the related market will expand. Prospect. The development of a convenient portable oral spray suitable for these needs is expected to increase sales volume and provide scientific data for product development for children and the silver generation. In addition, the market forecast is uncertain due to the weak connection between the market for natural raw materials related to oral health products and the market for applied products.

Republic of Korea Patent No. 10-1632910

The present invention provides a hypoallergenic, functional oral perfume using seaweed (Ecklonia cava, kelp, agar-agar, seaweed, seaweed, seaweed, seaweed, seaweed, gompkin, rhubarb, fusiformis, cockerel cap) and herbs, and a method for manufacturing the same aims to do

In order to achieve the above object, the hypoallergenic oral perfume composition using the seaweed complex and seawater as the main material of the present invention contains 12 kinds of seaweeds such as Ecklonia cava, kelp, agar, seaweed, seaweed, seaweed, shell, broad shell, and gomica. , Oral containing rhubarb, hijiki, and alpine capsicum as the seaweed material of the oral perfume composition, fragrant material made of sage (Salvia officinalis), and purified seawater containing minerals and bay salt (containing 4 degrees). Natural material preparation step to be used as a perfume composition component; After immersing 500g of raw seaweed in 25L of hot water at 40℃, shaking it manually from top to bottom for 1 hour, pouring it into a pot containing 5L of purified water and treating it with boiling water at 80℃ for 10 minutes, A pretreatment step for removing heavy metals from seaweed to remove heavy metals such as arsenic and cadmium by washing with cold purified water; After cutting the seaweed from which heavy metals were removed into small pieces, 200g of dry weight was dropped into 10 times the volume of purified water, extracted at 70℃ for 12 hours, and the extract was first filtered through 5 layers of gauze to remove the seaweed control pieces. Afterwards, a pure seaweed extract from which even minute foreign matter has been removed is prepared, and the liquid extract is placed in a 500mL square stainless steel container, subjected to low-temperature treatment at -20 ° C for 10 hours, and ultra-low temperature treatment at -70 ° C (Deepfreezer) for 12 hours, - Freeze-drying in a 70° C. Freeze dryer for 48 hours to prepare a heat export product from several kinds of seaweed; After freeze-drying, weigh 2g each of 12 types of seaweed extract frozen powder stored at low temperature at 4℃, then refreeze-dry in -70℃ Freeze dryer for 12 hours, and then use a mini mortar to make the freeze-dried extract a homogeneous powder. After crushing, fixing a beaker with a capacity of 300mL in a sonication bath at 50 ° C, warming 200mL of purified seawater in the beaker, and then adding 12 kinds of seaweed frozen powders sequentially at 30-minute intervals, each lyophilized powder While mixing, it is homogenized to prevent agglomeration, and freeze-dried powder of 12 kinds of seaweed-derived extracts is obtained. Gompi => rhubarb => halibut => hickory cap, and after completing the sonication mixing process, add seawater so that the final volume of the seaweed complex is 300mL, and then the seaweed mixture is placed on a hot plate at 40℃. After raising the beaker and dropping the magnetic bar on the bottom of the beaker, add 1mL of highly concentrated lime fermented liquid, stir at a speed of 30rpm for 10 hours, and process for 24 hours at 4℃ refrigeration to remove fine bubbles and finalize the physical properties of the seaweed complex. It is characterized in that it consists of preparing a complex using seaweed extract powder stabilized with and a pretreatment step for removing seaweed fishy smell.

According to the present invention, a hypoallergenic oral perfume manufacturing method with improved functionality using various seaweeds (Ecklonia cava, kelp, agar-agar, seaweed, seaweed, seaweed, shellfish, wide shell, gomkin, rhubarb, hijiki, and pine cap) as main materials to provide.

Looking at the research results on the physiological activity and oral health efficacy of seaweed used as an oral perfume material in this study,

*Green algae: Rich in Vt A and Vt C, antioxidant and anti-aging polyphenols suppress various bacteria, prevent periodontitis to protect gums, and have an effect of removing bad breath by chlorophyll contained in green algae.

* Ecklonia: Seanol, an extract of marine plant Ecklonia cava, was the first in Korea to be certified as a New Dietary Ingredient (NDI) by the FDA and successfully industrialized, and Botamedi, a domestic company, is developing and selling it as a super antioxidant.

Oral health efficacy of seaweed

type Physiological activity and oral health efficacy note Fucoidan derived from brown algae Inhibiting the growth of harmful oral bacteria Seanol derived from Ecklonia Excellent antioxidant activity (prevents premature aging of the gums) seaweed Antioxidant - Prevents periodontitis (gum care) / Chlorophyll - Removes bad breath mother and child class Anti-inflammatory activity (improvement of gum inflammation)

In the case of seaweed, various useful activities such as antithrombotic, antioxidant, anticancer, nitrite and heavy metal removal activity, prevention of hyperlipidemia and hypertension, antidiabetic, antimutation, inhibition of melanin formation, elastase inhibition and anti-inflammatory activity of polysaccharides such as fucoidans have been reported. Recently, studies on the antibacterial activity of seaweed are increasing. So far, the antibacterial activity of seaweeds has been tested against fish disease bacteria, anticariogenic bacteria, Staphylococcus aureus and E. have been reported in a limited manner.

Oral health effects of seaweeds (Ecklonia cava, kelp, agar-agar, seaweed, seaweed, seaweed, shellfish, seaweed, gompkin, rhubarb, fusiformis, and alpine caps): According to the research results of Kim et al. (Korean J Microbiol Biotechnol 40, 144-151, 2012), for E. coli, broad-leaved shell, shell, kelp, egg-shaped cap, cap-ban, pair-leaved cap, Yamada cap-ban, Japanese apricot, and flat-green algae are excellent. Antibacterial activity was shown, and among them, the growth inhibitory ring showed the largest size at 19.1 mm in plaque. , grape matsutake seaweed, leaf algae, and hole algae exhibited antibacterial activity, and among them, shells and hoeer caps showed strong antibacterial activity over 15.4 mm. However, the antibacterial activity against E. coli was not consistent. As a result of testing the antibacterial activity targeting typhimurium, antibacterial activity was shown in broadleaf, shellfish, eggshell, gambler, radiation patterned seaweed, flat seaweed and leaf seaweed, and in the case of leaf seaweed, very strong growth inhibition (20.3 mm) was shown. . For S. aureus, the extracts of Hijiki, Broadsword, Shell, Alsole caps, Caps caps, Caps capsae, Caps capsae, Caps. . For L. monocytogenes, seaweed, Ecklonia cava, gompkin, rhubarb, hijiki, broadtail, shellfish, kelp, earthworm, Yamada cap, ring hawk, seaweed, small bead coral end, black bead, grape cluster seaweed, deaf, flat seaweed, 19 species of seaweeds, including green leaf and green algae, showed antibacterial activity with growth inhibitory rings of 7.5 mm or more. Overall, plaque and broadleaf showed excellent antibacterial activity against various bacteria. 1. Meanwhile, for C. albicans, the causative bacterium of candidiasis skin infection, antifungal activity was shown in shellfish, broadtail, gomica, rhubarb, Ecklonia cava, fusiformis, alpine capsicum, and Yamada capsicum. was weak with a size of 7.0~7.5 mm. Against S. cerevisiae used in baking and brewing, antifungal activity was found only in shells and broadtails, and no seaweed showed antifungal activity against A. niger. Overall, brown and green algae extracts showed better antibacterial activity than red algae, and antifungal activity was especially shown in broad and shell.

Below, the dental care efficacy of hot-water extracts of Ecklonia cava, kelp, seaweed, and shellfish was analyzed, and Gamlon, kelp, agar-agar, seaweed, seaweed, seaweed, shell, wide shell, gompkin, rhubarb, hibiscus, and cockle hat were the main ingredients. The manufacturing method of the composed oral perfume composition and the composition will be described in detail.

1. Selection of natural materials to be used as components of oral perfume composition

- Seaweed material: 12 kinds of seaweed (economy cava, kelp, agar-agar, seaweed, seaweed, seaweed, scallop, wide scallop, gomica that are effective in inhibiting oral harmful bacteria, preventing dental caries, removing bad breath, improving stomatitis, and improving dry mouth. , rhubarb, fusiformis, and alpine caps) were used as seaweed materials for the oral perfume composition.

- Fragrance material (fragrance component): Sage (Salvia officinalis) was used as a representative fragrance material for oral perfume composition. It is effective in suppressing aging and relieving inflammation, and because of its antibacterial action, it is an effective herb for combating harmful microorganisms in the oral cavity.

- Refined sea water: When manufacturing oral perfume, seawater containing various minerals and sea salt (containing 4%) is used instead of purified water, so it is effective in preventing oral diseases through gum disease prevention and periodontal soft tissue convergence. .

2. Pretreatment for heavy metal removal from seaweed

After immersing 500g of raw seaweed in 25L of hot water at 40℃, shaking it manually from top to bottom for 1 hour, pouring it into a pot containing 5L of purified water and treating it with boiling water at 80℃ for 10 minutes, Heavy metals such as arsenic and cadmium were removed by washing with cold purified water.

3. Preparation of hot water extracts from several species of seaweed

After cutting the seaweed (Ecklonia cava, kelp, agar-agar, seaweed, seaweed, seaweed, seaweed, seaweed, gompkin, rhubarb, fusiformis, and agar cap) into small pieces, 200g of dry weight is purified by 10 times the capacity. After dropping in, extract at 70 ° C. for 12 hours. At this time, shaking was performed for 5 minutes periodically every 30 minutes.

The extract was first filtered through 5 layers of gauze to remove the seaweed control pieces, and then a pure seaweed extract from which even fine foreign substances were removed was prepared using Whatman filter paper No 1. The liquid extract was placed in a 500mL square stainless steel container, treated at -20℃ for 10 hours, ultra-low temperature treated at -70℃ (Deepfreezer) for 12 hours, and then freeze-dried in a -70℃ Freeze dryer for 48 hours to obtain pure seaweed extract powder. was prepared.

4. Preparation of complex using seaweed extract powder and pretreatment to remove fishy smell of seaweed

After freeze-drying, 12 types of seaweed extracts and frozen powders (Ecklonia cava, kelp, agar-agar, seaweed, seaweed, seaweed, shell, broadtail, gompkin, rhubarb, hijab, and pine caps), which are stored at a low temperature of 4℃, are weighed 2g each. After lyophilization in -70 ℃ Freeze dryer for 12 hours, the lyophilized extract was pulverized in a mini mortar to make a homogeneous powder. After fixing a beaker with a capacity of 300mL in a sonication bath at 50℃ and warming it by pouring 200mL of purified seawater into the beaker, 12 kinds of seaweed frozen powders were added sequentially at 30-minute intervals while each lyophilized powder was mixed. It was homogenized to prevent agglomeration.

The order of mixing the lyophilized powders of 12 kinds of seaweed-derived extracts is as follows. Gamtae => Kelp => Agar => Seaweed => Seaweed => Seaweed => Pile => Broadsword => Gompi => Rhubarb => Hijiki => Alsongi Hatban

After completing the sonication mixing process as described above, add seawater so that the final volume of the seaweed complex is 300mL, place the beaker containing the seaweed mixture on a hot plate at 40℃, drop the magnetic bar on the bottom of the beaker, and place the highly concentrated lime After adding 1 mL of the fermentation broth, stirring at a speed of 30 rpm for 10 hours, and processing for 24 hours in a 4°C refrigerated state to remove fine bubbles and finally stabilize the physical properties of the seaweed complex. The highly concentrated lime fermented liquid added in this process removes and offsets the fishy smell peculiar to the seaweed complex, thereby increasing the palatability of the oral perfume composition.

5. Analysis of the inhibitory ability of the seaweed complex against oral harmful bacteria (dental carious Streptococcus sobrinus)

< Inhibiting dental caries bacteria of Ecklonia cava extract >

<Seaweed complex inhibits dental caries bacteria>

<Inhibiting activity of cariogenic bacteria of kelp extract>

6. Verification of prevention of premature aging of gums and mitigation of inflammation through analysis of antioxidant activity of seaweed complex

< SOD-like activity: b- L, a- seaweed complex, c- kelp >

7. Verification of stomatitis and gingivitis prevention effect through analysis of anti-inflammatory activity of seaweed complex

(seaweed) (pae) (Ecklonia cava) (seaweed complex)

<Effect of seaweed extract on 4 inflammatory genes in RAW 264.7 cells treated with LPS>

8. Establishment of oral mist composition components and optimal mixing conditions

Ingredients used as antibacterial agents, sweeteners, fragrances, preservatives, pH regulators, and humectants were investigated for the constituents of the oral perfume composition, and the amount of addition was determined according to the chemical characteristics of each material type. In addition, differences in physical properties such as color, fragrance, and transparency were analyzed according to the amount of major natural materials (seawater, seaweed complex, herbs) and basic ingredients added.

In particular, the ‘Seaweed Complex’, which is composed of 12 kinds of seaweed materials, has antimicrobial activity, antioxidant activity, moisturizing function, and anti-inflammatory activity, so it is very useful as a hypoallergenic oral care product material. Herbs and herbal medicines, which are widely used as mouthwash or oral spray materials, have strong irritants, so seaweed extracts are used as a second material that can alleviate them, and seaweed complexes are very suitable as hypoallergenic materials.

Ingredients and efficacy of oral perfume composition

Blending purpose Name of raw material Mixing ratio (%) note solvent purified seawater 83.84 natural surfactant coconut oil 0.10 humectant D-sorbitol 10.00 (Agricultural) Glycerin 5.00 plaque removal, whitening baking soda 0.2 sweetener xylitol 0.100 Enzymatically Treated Stevia 0.050 pH regulator Citric acid (hydrate) 0.005 Adjust the pH of the solution to about 5.5 sodium citrate 0.30 natural food preservative (citrus) 0.10 coloring agent Blue No. 1 0.00014 use food coloring fragrance component sage spice 0.20 Use of edible flavoring agent natural emulsifier Olive Oil Wax 0.30 1.5 times the amount of mint flavor seaweed complex Ecklonia extract

1.0

antibacterial, antioxidant
promote salivation
Prevention of periodontal disease
dental caries prevention
bad breath improvement, antibacterial, antioxidant
Gums anti-aging effect Kelp Extract Agar-Agar Extract seaweed extract seaweed extract seaweed extract plaque extract broadleaf extract Gom bark extract rhubarb extract fusiformis extract egg yolk hat Sum 100.00

9. Design of portable mini spray bottle

Spray function by vacuum pressure/ Spray angle of 45 degrees/ Structure that does not leak when laid down/ Attached strip of material that adheres to the floor so that it does not fall when standing up

Claims (1)

12 kinds of seaweeds such as Ecklonia cava, kelp, agar-agar, seaweed, seaweed, seaweed, seaweed, seaweed, gomica, rhubarb, fusiformis, and cockerel cap are provided as seaweed materials for oral perfume composition, and Sage (Salvia officinalis) Preparing a natural material to be used as a component of an oral perfume composition having a fragrant material made of and having purified seawater containing minerals and bay salt (containing 4 degrees);
After immersing 500g of raw seaweed in 25L of hot water at 40℃, shaking it manually from top to bottom for 1 hour, pouring it into a pot containing 5L of purified water and treating it with boiling water at 80℃ for 10 minutes, A pretreatment step for removing heavy metals from seaweed to remove heavy metals such as arsenic and cadmium by washing with cold purified water;
After cutting the seaweed from which heavy metals were removed into small pieces, 200g of dry weight was dropped into 10 times the volume of purified water, extracted at 70℃ for 12 hours, and the extract was first filtered through 5 layers of gauze to remove the seaweed control pieces. Afterwards, a pure seaweed extract from which even minute foreign matter has been removed is prepared, and the liquid extract is placed in a 500mL square stainless steel container, subjected to low-temperature treatment at -20 ° C for 10 hours, and ultra-low temperature treatment at -70 ° C (Deepfreezer) for 12 hours, - Freeze-drying in a 70° C. Freeze dryer for 48 hours to prepare a heat export product from several kinds of seaweed;
After freeze-drying, weigh 2g each of 12 types of seaweed extract frozen powder stored at low temperature at 4℃, then refreeze-dry in -70℃ Freeze dryer for 12 hours, and then use a mini mortar to make the freeze-dried extract a homogeneous powder. After crushing, fixing a beaker with a capacity of 300mL in a sonication bath at 50 ° C, warming 200mL of purified seawater in the beaker, and then adding 12 kinds of seaweed frozen powders sequentially at 30-minute intervals, each lyophilized powder While mixing, it is homogenized to prevent agglomeration, and freeze-dried powder of 12 kinds of seaweed-derived extracts is obtained. Gompi => rhubarb => halibut => hickory cap, and after completing the sonication mixing process, add seawater so that the final volume of the seaweed complex is 300mL, and then the seaweed mixture is placed on a hot plate at 40℃. After raising the beaker and dropping the magnetic bar on the bottom of the beaker, add 1mL of highly concentrated lime fermented liquid, stir at a speed of 30rpm for 10 hours, and process for 24 hours at 4℃ refrigeration to remove fine bubbles and finalize the physical properties of the seaweed complex. A hypoallergenic oral perfume composition with a seaweed complex and seawater as the main materials, characterized in that it consists of preparing a complex using seaweed extract powder stabilized with and a pretreatment step for removing the fishy smell of seaweed.