KR20230036751A - Ultrasonic extract of fermentation liquid for skin health - Google Patents

Abstract

The present invention relates to an ultrasonic extract of a fermented liquid for skin health promotion, and a composition containing the same as an active ingredient. The composition containing a Phyllanthus emblica extract of the present invention as an active ingredient exhibits radical scavenging activity, antibacterial activity against acne-causing bacteria, an effect of reduction in inflammation-induced cytokine production, and simultaneously, an effect of reduction in the induction of skin damage.

Description

Ultrasonic extract of fermented liquid for skin health promotion {ULTRASONIC EXTRACT OF FERMENTATION LIQUID FOR SKIN HEALTH}

The present invention relates to an ultrasonic extract of a fermented liquid for promoting skin health and a composition containing the same as an active ingredient, and more specifically, an extract containing Amalaki ( Phyllanthus emblica ) as an active ingredient to treat acne and scars, pores, blemishes, It relates to a cosmetic, pharmaceutical composition or food composition for improving pigmentation, redness, pimples or skin diseases.

Skin health is important for maintaining a long healthy life, and is also an expression of beauty and youth, and there are a variety of products and treatment methods available to treat and/or prevent a variety of skin conditions.

However, many of these treatments use harsh prescriptions or procedures that have side effects, and mild treatments are not effective for everyone.

Examples of common skin conditions include acne or atopic dermatitis, which are caused by the action of hormones and other impurities in the skin's sweat glands and hair follicles. Regarding these diseases, about 85% of people are affected to some extent during their lifetime. Acne and atopic dermatitis are inflammatory diseases caused by blockage of the sebaceous glands and hair follicles, which can be caused by certain bacterial infections. Sebum provides a source of nutrients for the bacteria, creating an ideal environment for them to multiply and create comedones or "pimples."

Comedones are typically treated with creams or ointments containing active drug substances such as sebum secretion inhibitors, keratinization inhibitors, antibacterial agents and/or anti-inflammatory agents. However, many of these drug substances exhibit side effects. For example, mild inflammatory inflammation is commonly treated with over-the-counter topical medications, including benzoyl peroxide, salicylic acid, or retinoids. Benzoyl peroxide can leave an opaque or whitish residue on the skin and can be very drying to the skin. Also, benzoyl peroxide can bleach clothing and linens that come in contact with the treatment area. Moreover, many countries around the world, through government regulations, exclude or limit the use of benzoyl peroxide due to its potential toxicity. Alternatives to benzoyl peroxide are known, but these alternatives also present certain side effects. For example, estrogen and estrogen-like compounds such as ethinyl estradiol may sometimes be prescribed. These drugs are generally effective as sebum secretion suppressants, but may also cause nausea, headache, weight gain, It has been linked to mood swings, depression and other side effects.

In addition, some acne or atopy treatments contain antibacterial ingredients including chlorhexidine gluconate and benzalkonium chloride. These antimicrobials can cause skin irritation and cracking of the skin. In addition, antimicrobials can lead to resistant strains developing in wild-type fauna.

In addition, therapeutic agents such as salicylic acid and its salts are widely used to exfoliate dead skin cells and open and drain pores. Therapeutic agents have limitations, and at high concentrations at highly acidic pH, they can cause severe irritation. In addition, the treatment showed limited effect in directly reducing inflammation.

Accordingly, there is a need for safe and effective cosmetic treatment methods capable of treating and/or preventing a wide range of skin conditions including, for example, scars, pores, blemishes, pigmentation, redness, pimples and acne.

On the other hand, Amalaki ( Phyllanthus emblica ) is a small to medium-sized deciduous tree that is native to and cultivated throughout India. Its stem is curved, its gray soft bark peels off, its light green leaves are feathery and lemony, and its flowers are greenish-yellow. The fruit is ball-shaped, and when ripe, it is light yellow and shiny. In winter, it is beautiful greenish yellow in six pieces with a diameter of 1.5 to 3 cm.

Amalaki fruits contain 20 times more vitamin C than oranges. They are very heat stable, so even when exposed to high temperatures for a long time, the vitamin is almost not destroyed as when freshly harvested from the tree, even in dried fruits that are close to a year old. , It is believed that the durability and heat resistance of vitamin C in amalaki originate from the tannin component contained together. In addition, since this tannin turns black when combined with iron and salt, it is also used for manufacturing ink and hair dye (Korean Patent Publication No. 2017-0116486).

The fruits, leaves, seeds, roots, peels, and flowers of this plant are all used for medicinal purposes. The taste is initially bitter, but later sweet. good night. However, nothing has been disclosed or disclosed about amalaki ( Phyllanthus emblica ) in relation to acne and scars, pores, blemishes, pigmentation, redness, pimples, or skin disease improvement.

Accordingly, while the present inventors were researching a composition for skin health, Amalaki ( Phyllanthus emblica ), Geranium ( Geranium ), and Googul ( Commiphora myrrha ) from a mixture using acne and scars, pores, blemishes, pigmentation, redness, The present invention was completed by confirming that pimples or skin diseases could be improved.

An object of the present invention is to provide a composition for improving acne and scars, pores, blemishes, pigmentation, redness, pimples or skin diseases using an ultrasonic extract of a fermentation broth containing an extract containing Phyllanthus emblica as an active ingredient. there is.

The above and other objects of the present invention can all be achieved by the present invention described below.

In order to achieve the above object, the present invention

Amalaki ( Phyllanthus emblica ), Geranium ( Geranium ) and Googul ( Commiphora myrrha ) in the solid phase dry matter under a polar protic solvent Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus plantarum ( Lactobacillus plantarum ), lactose Bacillus bulgaricus ( Lactobacillus bulgaricus ), Enterococcus faecium ( Enterococcus faecium ), Bifidobacterium longum ( Bifidobacterium longum ) and Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) It provides a skin health promoting composition containing, as an active ingredient, an ultrasonic extract of a fermentation broth obtained by inoculating one or more fermented strains selected from.

The ultrasonic extraction may be performed for 10 to 180 minutes at a frequency of 25 to 75 kHz at 20 to 70 °C.

The polar protic solvent may include at least one selected from water, chloroform, ethanol, methanol, ethyl acetate, nucleic acid, and diethyl ether in an amount of 5 to 20 times the dry weight of the solid phase.

The amalaki ( Phyllanthus emblica ), geranium ( Geranium ), and guggul ( Commiphora myrrha ) may satisfy a weight ratio of 1: 0.75 to 4: 0.75 to 4 in amalaki (a): geranium (b): guggul (c).

The ultrasonic extract may be extracted for 2 to 3 hours at 60 to 70 ° C. under reflux.

The skin health can provide an effect of improving acne, scars, pores, blemishes, pigmentation, redness, pimples or skin diseases.

According to the present invention, whitening and blemish effect using ultrasonic extract of amalaki-derived ultrasonic extract as an active ingredient, which simultaneously expresses radical scavenging activity, antibacterial activity against acne-causing bacteria, reduction of inflammation-induced cytokine production, and reduction of skin damage. A composition for preventing and treating age spots, freckles, melasma, and keratin can be provided.

1 is a graph comparing DPPH and ABTS radical scavenging activities for four types of samples (PGC-4) obtained in Example 1 and samples (PGC-1 to PGC-3) obtained in Comparative Examples 1 to 3 .
Figure 2 is a graph comparing the cytotoxicity of four types of samples (PGC-4) obtained in Example 1 and samples (PGC-1 to PGC-3) obtained in Comparative Examples 1 to 3.
Figure 3 is a graph comparing the LPS pro-inflammatory cytokine evaluation results for four types of samples (PGC-4) obtained in Example 1 and samples (PGC-1 to PGC-3) obtained in Comparative Examples 1 to 3 am.

Hereinafter, the present invention will be described in more detail to aid understanding of the present invention.

Terms or words used in this specification and claims should not be construed as being limited to their usual or dictionary meanings, and given that the inventors may appropriately define the concept of terms in order to best describe the invention. Therefore, it should be interpreted as meaning and concept consistent with the technical spirit of the present invention.

As confirmed in the following Examples and Experimental Examples, the present invention prepares a solid phase dried product using Phyllanthus emblica , Geranium and Commiphora myrrha , and polar protons therein. When the extract is prepared by ultrasonicating the fermented liquid obtained by inoculating the fermented strain using a solvent, radical scavenging activity, antibacterial activity against acne-causing bacteria, reduction of inflammation-induced cytokine production, and reduction of skin damage induction are simultaneously expressed. By doing so, it is possible to provide a composition for preventing and treating whitening, blemish removal, age spots, freckles, melasma, and keratin.

The hot water ultrasonic extract, ethanol ultrasonic extract, or fermented hot water ultrasonic extract did not show radical scavenging activity, antibacterial activity against acne-causing bacteria, reduction of inflammation-induced cytokine production, and reduction of skin damage induction at the same time. Although not shown in the examples, Amalaki ( Phyllanthus emblica ) content, geranium ( Geranium ) content, and guggul ( Commiphora myrrha ) 1: 0.75 ~ 4: even if the weight ratio of 0.75 ~ 4 is not satisfied, the radical Clearing activity, antibacterial activity against acne-causing bacteria, inflammation-inducing cytokine production reduction effect, and skin damage induction reduction effect were not shown at the same time or the effect was reduced.

The composition of the present invention that simultaneously realizes the radical scavenging activity, antibacterial activity against acne-causing bacteria, reducing the amount of inflammatory cytokine production, and reducing the induction of skin damage is provided based on these experimental results, and promotes skin health according to the present invention. The composition is obtained by mixing Phyllanthus emblica with Geranium and Commiphora myrrha in a weight ratio of 1:0.75 to 4:0.75 to 4, and then adding a polar protic solvent to the obtained solid phase product. It is characterized in that it contains the ultrasonic extract of the fermentation broth obtained by inoculating the fermentation strain using the active ingredient.

The skin health promoting composition of the present invention is preferably a solid obtained after mixing amalaki ( Phyllanthus emblica ) with Geranium and Commiphora myrrha at a weight ratio of 1:0.75-4:0.75-4 phase product) as an active ingredient.

In the present description, unless otherwise specified, an active ingredient means a component that exhibits the desired activity alone or that can exhibit activity in combination with a carrier that is not active by itself.

In the present description, unless otherwise specified, the extract refers to an extract obtained by inoculating a fermenting strain using a polar protic solvent from the solid material itself to be extracted, or a fraction obtained by fractionating the extract. In consideration of polarity, degree of extraction, and degree of preservation, methods such as cooling, reflux, heating, ultrasonic radiation, supercritical extraction, ultrahigh pressure extraction, fermentation, and hydrolysis may be applied. In the case of a fractionated extract, the fraction or crude extract obtained by suspending the extract in a specific solvent, mixing with a solvent of different polarity, and standing still is adsorbed on a column filled with silica gel, etc. It means including the fraction obtained by.

In the present disclosure, the shape of the solid material is not particularly limited, and may be in the form of pills, granules, or powder.

The solid material may be obtained in the form of a solid material after thoroughly washing Amalaki ( Phyllanthus emblica ), Geranium ( Geranium ) and Guggul ( Commiphora myrrha ). After cleaning, it can be dried by any method such as natural drying (shade drying) or hot air drying to remove some of the moisture, and then crushed and then used for ultrasonic treatment.

In the present invention, unless otherwise specified, geranium and guggul means obtained by extracting from various organs or parts (eg leaves, branches, flowers, roots, stems, fruits, etc.), preferably from each leaf or fruit. means the obtained extract.

Geranium ( Pelargonium graveolens ) is a perennial plant belonging to the family Graveolaceae, originating from Egypt, Morocco, Algeria, and China. The stem is 30-50 cm high and fleshy. there is The flower blooms in summer and has a stalk at the end of a long peduncle and runs in an umbel. Before the flower blooms, the flower bud droops downward and then opens upward, and the color of the flower varies depending on the variety. There are 5 sepals and petals, 10 stamens, and 1 pistil with 5 ovaries.

The main ingredients are geraniol, citronellol, linalol, citronellyl formate, and metone. Known effects include emotional mood elevation, depression, anxiety, behavior disorder and stress relief, skin convergence, and sebum secretion normalization. It is effective in relieving inflammation, removing dry eczema, acne, removing acne, and relieving wrinkles, and has a tonic and cleansing effect on the digestive system, such as treating oral ulcers, diarrhea, and gastroenteritis. In the circulatory system, it removes waste products, relieves fluid retention, and regulates blood pressure. In the respiratory system, it relieves oral infections through the antibacterial action of cold viruses. Gynecologically, it stimulates the adrenal cortex and regulates hormones to relieve menstrual disorders, premenstrual syndrome, menopausal symptoms, vaginitis, infertility, and breast swelling. (Aromatherapy Handbook p101, 2002, both sides)

In addition, guggul is a medicinal herb of the olive family and can be used for medicinal purposes. In oriental medicine, it is known to have efficacies such as the effect of releasing congestion due to stagnation of energy, the effect of removing acid and blood, and the effect of eradicating swelling and eliminating pain. It is used for the treatment of wounds caused by injury to muscles and bones with blades, injuries from falling or bumping, overall pain in the chest and stomach, and sprains between organs and bones due to qi and blood being blocked by venomous snakes. .

In addition, it can be used in the form of pulverized material or in a dried state after steaming in stages, and when used in a dried state after steaming in stages, it is possible to provide the effect of increasing the usefulness of storage and active ingredients.

Any method, such as diluting the dry matter of the solid phase with a polar protic solvent before fermentation, followed by stirring at 50 to 85 ° C. for 24 hours or less, may be provided.

The polar protic solvent may be, for example, water or alcohol having 1 to 3 carbon atoms, and it is preferable to use water in consideration of simple treatment of the solvent.

The polar protic solvent may be 5 to 20 times, specifically 5 to 15 times, based on the dry weight of the solid phase. In this case, the subsequent fermentation process can be effectively performed.

For example, the fermentation strain is Lactobacillus acidophilus , Lactobacillus plantarum , Lactobacillus bulgaricus , Enterococcus faecium , Bifidobacterium longum ( Bifidobacterium longum ) and Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) It may be one or more selected from.

The fermentation strain may be a mixture of Lactobacillus acidophilus (KCCM 32870), Lactobacillus plantarum (KCCM 11322) and Lactobacillus bulgaricus (KCCM 40266 ).

In the present description, the fermenting strain is added at 10 ⁴ to 10 ⁷ CFG/g, specifically 10 ⁵ to 10 ⁶ CFG/g.

The incubation temperature is 27 to 37 ° C. for normal microbial culture conditions, and the pH is 5 to 7 for aerobic or usually anaerobic conditions.

In the present invention, the fermenting strain is obtained by inoculating and culturing lactic acid bacteria in a medium containing carbon sources such as lactobacillus activated by Phyllanthus emblica extract, specifically Amalaki water (including hot water) extract and sugar before the inoculation. can also be used Although not presented in the following experimental examples, the present inventors believe that fermentation will proceed to the depths of the dry matter (ground material) in the solid phase when using lactic acid bacteria activated with amalaki water (including hot water) extract. Lactic acid bacteria activated by the Amalaki water (including hot water) extract can be obtained by preparing a medium containing the Amalaki water extract and a carbon source and inoculating the lactic acid bacteria therein, wherein the carbon source is any carbon source known in the art, For example, oligosaccharides, lactose, glucose, fructose, sucrose, sugars, dextrose, starch, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose and mixtures thereof This may be used, and may be in the range of 1 to 10% (w / v), which is the amount added to the medium, and the medium may further include a nitrogen source or trace elements in the range of 0.1 to 3% (w / v), as a nitrogen source may include ammonia, ammonium salt, urea, amino acids, soybean meal, soybean protein, yeast extract, broth, etc., and trace elements include K, Mg, Na, Co, Mo, Ca, Mn, Zn, Cu, Fe, P, S etc. are mentioned. In addition, the culture may be performed at 25 to 40 °C or 35 to 37 °C for 12 hours to 2 days.

In the present invention, the culturing after the inoculation may be performed at 15 to 45 °C, 25 to 40 °C or 25 to 35 °C. If the culture temperature is lower than the above range, the fermentation rate may be slowed down or unwanted by-products may be produced, and even if the culture temperature is higher than the above range, the fermentation rate may be slowed down or unwanted fermentation by-products may be produced as the fermentation microorganisms die. . The culturing time may be about 1 to 7 days, or about 2 to 5 days.

After the fermentation, the supernatant may be extracted by filtration and centrifugation according to any known method, and sonication may be performed.

The ultrasonic treatment is a treatment method using energy generated by ultrasonic vibration, and ultrasonic waves can destroy the insoluble solvent contained in the sample, and the high local temperature generated at this time greatly increases the kinetic energy of reactant particles located around it. Therefore, sufficient energy required for the reaction is obtained, and high pressure is induced by the impact effect of ultrasonic energy to increase the mixing effect of the substances contained in the sample and the solvent, thereby increasing the extraction efficiency.

The ultrasonic extraction may be carried out for 10 to 180 minutes at a frequency of 25 to 75 kHz at 20 to 70 ° C, for example. In this case, it is possible to provide an increase in effective substance extraction efficiency. Specifically, the ultrasonic extraction may be performed for 30 to 90 minutes at a frequency of 25 to 35 to 60 kHz at 35 to 60 °C.

Regarding the extraction solvent used in the ultrasonic extraction method, in the case of the present invention, since the extraction solvent sufficient for inoculation of the fermenting strain was used, there is no need to use it separately.

Then, in the present description, reflux extraction of the sonicated liquid with ethanol is appropriate for optimally extracting active ingredients.

The ethanol reflux extraction is known to be highly efficient because it can remove impurities and extract active ingredients with high purity, but the properties of active ingredients can be changed depending on the temperature and pressure conditions of extraction and the extraction solvent.

For example, when the ultrasonic extract is extracted under hot water reflux conditions, as shown in Comparative Example 1 or Comparative Example 3 described later, the concentration of sugar in the extract is increased due to a decrease in the solubility of the active ingredient. In this extraction method, it is possible to adjust the content of free fatty acids and the content of active ingredients by adjusting the fractionation time. There is a way to lower the degree of concentration by mixing the sections.

The free fatty acid content of raw materials varies depending on the country of origin or cropping, and since raw materials with low acid values are usually formed at high prices, they are less competitive as raw materials. In addition, since there is no raw material without free fatty acids, free fatty acids are concentrated in the extract concentration section, so even if a product is made by setting a standard for using raw materials with a low acid value, it is easy to meet the acid value standard while satisfying the active ingredient content at a certain concentration or higher. It's not work. Therefore, in order to have industrial stability in the process of making a high-concentration fraction using an ultrasonic extract, a technique for removing free fatty acids from the reflux-extracted fraction is required.

According to the present invention, the reflux extraction is performed under reflux at 20 to 70 ° C., or 35 to 60 ° C. for 18 hours or less, or 1 to 12 hours to provide an extract with a maximized content of active ingredients and a reduced content of free fatty acids. It can be extracted repeatedly. The number of repetitions of extraction may be 5 times or less, for example, 2 to 3 times.

A purification process that removes the above-mentioned free fatty acids without affecting the efficacy without loss of active ingredients is required. For this purpose, a method of filtering using a fine filtration membrane and then concentrating under reduced pressure to remove free fatty acids is preferred, and more excellent efficacy It is more preferable to use a filtration membrane of 1.0 μm or less, preferably 0.45 μm or less, for providing the effect.

An effective extract can be obtained through the above-described purification process.

In the present description, the effective extract includes a concentrated liquid extract or solid extract from which the extraction solvent introduced prior to ultrasonic extraction is removed by freeze drying, vacuum drying, hot air drying, spray drying, vacuum concentration, or the like.

In the present disclosure, the form of the resulting extract is not particularly limited and may be in the form of a powder or other extract, including pills, granules, and powders.

Extract manufacturing method according to the present description, for example, Amalaki ( Phyllanthus emblica ) And, geranium ( Geranium ) And guggul ( Commiphora myrrha ) Cleanly washed and then inoculated with a fermenting strain in the obtained solid or dried product; and ultrasonically extracting the fermentation broth.

In the present description, the amalaki ( Phyllanthus emblica ), geranium ( Geranium ), and guggul ( Commiphora myrrha ) can be obtained in the form of solids after being washed thoroughly. After cleaning, some of the moisture is removed by drying in an arbitrary method such as natural drying (shade drying) or hot air drying (in the form of pulverized material so that the moisture content of the solid material is 10% (w / w) or less) 10 to 10 It may be crushed to 50 mesh, specifically 30 to 50 mesh to increase the surface area and then used for ultrasonic pretreatment.

If necessary, it may be pulverized after drying at 95° C. or less, or 50 to 90° C. for 36 hours or more, or 48 hours or more.

The solid may be subjected to a fermentation extraction step prior to ultrasonic extraction.

There is no particular limitation on the solids used in this description, and the fermentation and extraction step is for removing harmful substances such as microorganisms, residual pesticides, and preservatives on the surface of the solids or their dried products (also referred to as 'solid dried materials'). treatment, treatment to increase the contact area of ultrasonic waves, and the like.

In the case of the fermentation extraction, it is preferable to increase the treatment efficiency by supplying a polar protic solvent to the dried solid phase. One or more selected species may be used. At this time, the polar protic solvent may be included in an amount of 5 to 20 times the weight of the solid dry material.

In the present invention, the fermenting strain is a mixture of Lactobacillus acidophilus (KCCM 32870), Lactobacillus plantarum (KCCM 11322) and Lactobacillus bulgaricus (KCCM 40266 ) used in the following examples. Preferred to provide a composition for promoting skin health, In addition, Enterococcus faecium ( Enterococcus faecium ), Bifidobacterium longum ( Bifidobacterium longum ) and Leuconostoc mesenteroides and the like can also be used as an alternative or combined.

In the present invention, the culturing after the inoculation may be performed at 15 to 45 ° C. If the culture temperature is lower than the above range, the fermentation rate may be slowed down or unwanted by-products may be produced, and even if the culture temperature is higher than the above range, the fermentation rate may be slowed down or unwanted fermentation by-products may be produced as the fermentation microorganisms die. . The culturing time may be about 1 to 7 days, or about 2 to 5 days.

The ultrasonic treatment is preferably carried out for 10 to 180 minutes at a frequency of 25 to 75 kHz, for example, to increase treatment efficiency.

Ultrasonic extraction followed by reflux extraction under reflux can be performed. At this time, extraction may be repeated for 12 hours or less at 20 to 70 ° C., for 1 to 6 hours as a specific example. The number of repetitions of extraction may be 5 times or less, for example, 2 to 3 times.

The reflux extract may be purified and concentrated under reduced pressure as described above to remove the extraction solvent provided during reflux extraction and separate an effective extract.

In addition to the active ingredient, the composition of the present invention may be prepared by including a skin whitening component, a skin wrinkle improvement component, a skin elasticity improvement component, a UV protection component, a skin moisturizing component, etc. known in the art in order to reinforce or add skin-related activities. can

Examples of the skin whitening component include arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol, oil-soluble licorice extract, and the like.

Examples of the skin wrinkle improvement component include retinol, retinyl palmitate, adenosine, and polyethoxylated amide.

As the ultraviolet protection component, complexes such as drometrizol, drometrizoletrisiloxane, digaloyltrioleate, dimethicodiethylbenzalmalonate, diethylhexylbutamidotriazone, pine bark extract, phosphatidylserine, finger and complex extracts such as root extract powder, red ginseng and cornus officinalis.

As the moisturizing ingredients, AP collagen enzyme decomposition peptide, collagen peptide, N-acetylglucosamine, konjac potato extract, complex extracts such as dandelion, rice bran extract, corn embryo extract, low molecular weight collagen peptide, weed extract powder, phosphatidylserine, hyaluronic acid, etc. can be heard

For other skin whitening ingredients, wrinkle improvement ingredients, UV protection ingredients, and skin moisturizing ingredients, the Functional Cosmetics Code according to the Cosmetics Act (“Functional Cosmetics Standards and Test Methods” in the Ministry of Food and Drug Safety), and the Health Functional Food Code of the Act on Health Functional Foods (The Ministry of Food and Drug Safety Notice "Health functional food standards and standards" can be referred to. These ingredients may be included in the composition of the present invention one or more together with the active ingredient.

The composition of the present invention may contain the active ingredient in any amount (effective amount) as long as it can exhibit antioxidant effects, whitening effects, keratin exfoliation effects, etc. depending on the product type, product formulation, etc., and the effective amount is the total weight of the composition. It can be determined within the range of 0.001 to 15 wt% based on.

In the present invention, the effective amount, unless otherwise specified, when the composition of the present invention is administered to a mammal, preferably a human, during the administration period suggested by a medical expert, etc., has an antioxidant effect, a whitening effect, and a keratin exfoliation effect. It refers to the amount of the active ingredient included in the composition of the present invention, which can exhibit the intended medical/cosmetic effect, etc. Such an effective amount can be determined empirically within the ordinary skill of the skilled artisan.

In a specific aspect, the composition of the present invention can be identified as a cosmetic composition.

Even when the composition of the present invention is identified as a cosmetic composition, the cosmetic composition may be classified as an arbitrary product in terms of its use and law, and specifically, it may be a functional cosmetic having a purpose such as skin whitening, a non-functional general cosmetic, etc. .

In product form, it may take any product form, specifically, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax It can be formulated as a foundation, spray, and the like. In a specific product form, it may be a softening lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder formulation.

The cosmetic composition of the present invention may include, in addition to the active ingredient, ingredients commonly used in cosmetic compositions, for example, stabilizers, solubilizers, surfactants, vitamins, auxiliary agents such as pigments and fragrances, and carriers.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, fatty acid esters of sorbitan, and the like can be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar, and the like can be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention may be prepared according to a method for preparing a cosmetic composition conventionally performed in the art, except for including an active ingredient exhibiting skin wrinkle improvement activity, skin elasticity improvement activity, and the like.

In another specific aspect, the composition of the present invention can be regarded as a food composition.

The food composition of the present invention can be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, and ionic beverages, processed oils such as milk and yogurt, chewing gum, rice cakes, traditional Korean snacks, bread, snacks, noodles, etc. It can be manufactured into health functional food preparations such as food, tablet, capsule, pill, granule, liquid, powder, flake, paste, syrup, gel, jelly, bar, etc.

In addition, the food composition of the present invention may take any product classification as long as it conforms to the enforcement regulations at the time of manufacture / distribution in functional classification. For example, it may be a health functional food according to the Health Functional Food Act, or soybean milk, fermented beverages, special purpose food, etc. according to each food type in the Food Code (MFDS notification, food standards and specifications) of the Food Sanitation Act.

The food composition of the present invention may include food additives in addition to the active ingredient. Food additives are generally understood as substances that are added to, mixed with, or infiltrated into food in manufacturing, processing, or preserving food, and since they are taken daily and for a long period of time with food, their safety must be guaranteed. According to the Food Additive Code (Ministry of Food and Drug Safety Notice, Food Additive Standards and Specifications) under the Food Sanitation Act, safety-guaranteed food additives are classified into chemical synthetic products, natural additives, and mixed formulations and are limitedly regulated.

These food additives can be classified into sweeteners, flavors, preservatives, emulsifiers, acidulants, thickeners, etc. in terms of functionality.

Sweeteners can be used in amounts that give the food a moderately sweet taste, and can be natural or synthetic. Preferably, a natural sweetener is used, and examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavors may be used to improve taste or aroma, and both natural and synthetic flavors may be used. Preferably, it is the case of using a natural one. In case of using natural ones, in addition to flavor, the purpose of enhancing nutrition can also be combined. As a natural flavoring agent, it may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, etc., or obtained from green tea leaves, roundworms, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. In addition, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo, etc. can also be used. Natural flavors can be liquid concentrates or solid extracts.

Synthetic flavors may also be used, and synthetic flavors may include esters, alcohols, aldehydes, terpenes, and the like.

As preservatives, sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc. may be used, and as emulsifiers, acacia gum, carboxymethylcellulose, xanthan gum , pectin, and the like.

As the acidulant, acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like may be used. Acidulants may be added to the food composition to have an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to improving taste.

A suspending agent, a sedimentation agent, a gel forming agent, a swelling agent, and the like may be used as the thickening agent.

The food composition of the present invention, in addition to the above-mentioned food additives, may include physiologically active substances or minerals known in the art for the purpose of supplementing or reinforcing functionality and nutrition and ensuring stability as food additives.

Examples of such physiologically active substances include catechins contained in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoylthiamine, and the like. Examples of minerals include calcium preparations such as calcium citrate and magnesium stearate. magnesium preparations such as the like, iron preparations such as iron citrate, chromium chloride, potassium iodine, selenium, germanium, vanadium, zinc, and the like.

The food composition of the present invention may include the above-mentioned food additives in an appropriate amount to achieve the purpose of addition according to the type of product. Regarding other food additives that may be included in the food composition of the present invention, reference may be made to the Food Codex or Food Additives Codex.

In a specific embodiment, the composition of the present invention can be identified as a pharmaceutical composition.

The pharmaceutical composition of the present invention may be formulated into an oral formulation or a parenteral formulation according to the route of administration by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient.

In the present invention, pharmaceutically acceptable means that, unless otherwise specified, it does not inhibit the activity of the active ingredient and does not have toxicity more than is adaptable to the subject of application (prescription).

When the pharmaceutical composition of the present invention is prepared as an oral dosage form, powder, granule, tablet, pill, dragee, capsule, liquid, gel, syrup, suspension, wafer according to a method known in the art together with a suitable carrier. It can be prepared in formulations such as

Suitable pharmaceutically acceptable carriers include, for example, sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol, starches such as corn starch, potato starch, and wheat starch, cellulose, methylcellulose, and ethyl Cellulose, sodium carboxymethylcellulose, cellulose such as hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol , vegetable oil, and the like. If necessary, diluents and/or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants may be included in the formulation.

When the pharmaceutical composition of the present invention is prepared as a parenteral formulation, it may be formulated in the form of an injection, transdermal administration, nasal inhalation, and suppository along with a suitable carrier according to a method known in the art.

When formulated as an injection, suitable carriers include sterile water, ethanol, polyols such as glycerol or propylene glycol, or mixtures thereof, preferably Ringer's solution, PBS (phosphate buffered saline) containing triethanolamine or sterilization for injection water, isotonic solutions such as 5% dextrose, and the like can be used.

When formulated as a transdermal formulation, it may be formulated in the form of an ointment, cream, lotion, gel, external agent, pasta, liniment, or air roll.

In the case of nasal inhalation, it may be formulated in the form of an aerosol spray using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, or the like.

Bases for suppositories include witepsol, tween 61, polyethylene glycols, cacao fat, laurin fat, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, and sorbitan fatty acid esters. can be used

Formulation of pharmaceutical compositions is known in the art, and may be specifically referred to in Remington's Pharmaceutical Sciences (19th ed., 1995). These documents are considered as part of this specification.

The preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.001 mg/kg to 10 g/kg per day, preferably 0.001 mg/kg to 10 g/kg per day, depending on the patient's condition, body weight, sex, age, severity of the patient, and route of administration. It may be in the range of 1 g/kg. Administration can be done once a day or divided into several times. These dosages should not be construed as limiting the scope of the present invention in any respect.

The cosmetic composition, food composition, and pharmaceutical composition of the present invention may be prepared according to the preparation method of the composition commonly performed in the art, except for including active ingredients known in the art.

In describing the skin health promoting composition of the present disclosure, other conditions or equipment not explicitly described may be appropriately selected within the range commonly practiced in the art, and are not particularly limited.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Example]

<Example 1> Preparation of Fermented Ultrasonic Amalaki Extract

(1) Amalaki ( Phyllanthus emblica ) fruits, geranium ( Geranium ) leaves and guggul ( Commiphora myrrha ) fruits were thoroughly washed, dried at 60 ° C. for 48 hours, and pulverized to a size of 40 mesh or less. The pulverized raw materials were mixed at a constant weight (100 g: 100 g: 100 g).

30 _ ^_ After fermentation for 3 days at ° C., the supernatant was obtained by centrifugation.

(3) The obtained fermentation broth was treated at 50° C. at a frequency of 50 kHz for 2 hours using a sonicator.

(4) Then, 70% ethanol 10 times the weight of the ultrasonic extract was added and reflux extraction was performed at 60 ° C. for 6 hours three times.

(5) The obtained extract was filtered through a 0.45 μm membrane filter and then ethanol was removed using a vacuum concentrator to prepare a fermented ultrasonic amalaki extract (sample PGC-4).

<Comparative Example>

<Comparative Example 1> Preparation of Hot Water Ultrasonic Amalaki Extract

(1) Amalaki ( Phyllanthus emblica ) fruits, geranium ( Geranium ) leaves and guggul ( Commiphora myrrha ) fruits were thoroughly washed, dried at 60 ° C. for 48 hours, and pulverized to a size of 40 mesh or less. The pulverized raw materials were mixed at a constant weight (100 g: 100 g: 100 g).

(2') Water 10 times the total weight of the mixed solid dry matter was added.

(3') Then, it was treated for 2 hours at a frequency of 50 kHz at 50 °C using a sonicator.

(4') Subsequently, reflux extraction was performed at 60 °C for 6 hours by repeating three times.

(5) The obtained extract was filtered through a 0.45 μm membrane filter and then ethanol was removed using a vacuum concentrator to prepare a hot water ultrasonic amalaki extract (sample PGC-1).

<Comparative Example 2> Preparation of Ethanol Ultrasonic Amalaki Extract

(1) Amalaki ( Phyllanthus emblica ) fruits, geranium ( Geranium ) leaves and guggul ( Commiphora myrrha ) fruits were thoroughly washed, dried at 60 ° C. for 48 hours, and pulverized to a size of 40 mesh or less. The pulverized raw materials were mixed at a constant weight (100 g: 100 g: 100 g).

(2") 70% ethanol 10 times the total weight of the mixed solid dry matter was added.

(3') It was treated for 2 hours at a frequency of 50 kHz at 50 °C using a sonicator.

(4') Then, reflux extraction was performed three times at 60 °C for 6 hours.

(5) The obtained extract was filtered through a 0.45 μm membrane filter, and ethanol was removed using a vacuum concentrator to prepare an ethanol ultrasonic amalaki extract (sample PGC-2).

<Comparative Example 3> Preparation of fermented hot water ultrasonic amalaki extract

(1) Amalaki ( Phyllanthus emblica ) fruits, geranium ( Geranium ) leaves and guggul ( Commiphora myrrha ) fruits were thoroughly washed, dried at 60 ° C. for 48 hours, and pulverized to a size of 40 mesh or less. The pulverized raw materials were mixed at a constant weight (100 g: 100 g: 100 g).

30 _ ^_ After fermentation for 3 days at ° C., the supernatant was obtained by centrifugation.

(3) The obtained fermentation broth was treated at 50° C. at a frequency of 50 kHz for 2 hours using a sonicator.

(4”) Then, reflux extraction was performed by adding water 10 times the weight of the ultrasonic extract and repeating it three times for 6 hours at 60 ° C.

(5) The obtained extract was filtered through a 0.45 μm membrane filter and then ethanol was removed using a vacuum concentrator to prepare a fermented hot water ultrasonic amalaki extract (sample PGC-3).

<Experimental example>

<Experimental Example 1> Antioxidant activity test

ABTS method and DPPH method radical scavenging ability tests were performed to evaluate the radical scavenging ability of the samples by antioxidant tests on the samples obtained in Example 1 and the samples (PGC-1 to 4) obtained in Comparative Examples 1 to 3.

(1-1) ABTS radical scavenging activity

Each of the samples (PGC-1 to PGC-4) is diluted in water to prepare test solutions with concentrations of 100 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL. 7 mM ABTS and 2.45 mM potassium persulfate are mixed and reacted at room temperature for 12 hours in a dark room to form ABTS cations. Thereafter, ethanol was added to adjust the absorbance value at 734 nm to be 0.70 ± 0.02.

100 μl of the test solution and 100 μl of the prepared ABTS solution were added to a 96-well plate, reacted at room temperature for 7 minutes, and measured at 734 nm. Compared with the blank test solution, the ABTS clearance rate was obtained as a percentage (%) as follows and is shown in FIG. 1 below.

ABTS radical scavenging ability (%) = [Control - (Sample - Blank)]/Control Х 100

(However, Control: absorbance of ABTS reagent, Sample: absorbance of Sample + ABTS reagent, Blank: absorbance of Sample + Blank)

(1-2) DPPH radical scavenging activity

Each of the samples (PGC-1 to PGC-4) is diluted in water to prepare test solutions with concentrations of 100 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL. 100 μl of the test solution and 100 μl of 0.2 mM DPPH were put in a 96-well plate, and after 30 minutes, the absorbance was measured at 517 nm using a microplate reader. Compared to the blank test solution, the DPPH radical scavenging rate was obtained in percentage (%) as follows and is shown in FIG. 1 below.

DPPH radical scavenging ability (%) = [Control - (Sample - Blank)]/Control Х 100

(Control: Absorbance of DPPH reagent, Sample: Absorbance of Sample + DPPH reagent, Blank: Absorbance of Sample + Blank)

As shown in FIG. 1, as a result of ABTS radical analysis, ascorbic acid as a control group was found to be the highest at 99.5% at 1000 μg/mL, followed by 83.5% at 1000 μg/mL in the sample (PGC-4) according to Example 1 It was confirmed as high, followed by 1000 μg / mL in the case of the sample according to Comparative Example 3 (PGC-3), the sample according to Comparative Example 2 (PGC-2), and the sample according to Comparative Example 1 (PGC-1) 73.4%, 70.9%, and 64.5%, respectively.

In addition, as a result of the DPPH radical analysis, the same tendency as the above-mentioned ABTS analysis result was shown. Specifically, the sample according to Example 1 (PGC-4), the sample according to Comparative Example 3 (PGC-3), the sample according to Comparative Example 2 (PGC-2), and the sample according to Comparative Example 1 (PGC-1 ) showed 88.7%, 84.5%, 70.9%, and 64.5% at 1000 μg/mL, respectively. At this time, ascorbic acid as a control was analyzed at 99.4% at 1000 μg/mL.

<Experimental Example 2> Antibacterial activity test

As an antibacterial test on the samples obtained in Example 1 and the samples (PGC-1 to 4) obtained in Comparative Examples 1 to 3, the antibacterial activity test of acne-causing bacteria was performed by the paper disc method.

The acne-causing bacteria were Propionibacterium acnes (KCTC 3314), which was pre-sold from the Center for Biological Resources (KCTC), cultured in Reinforced Closetridial broth medium at 37°C, and used in experiments.

The cultured acne-causing bacterium Propionibacterium acnes (KCTC 3314) was smeared at 1 Х 10 ⁶ CFU/mL on a solid medium (agar 20 g/L) in an amount of 100 μl using a sterile cotton swab. Test materials (PGC-1~4) were diluted in water to prepare a concentration of 500 μg/mL, and 20 μl per disc was slowly absorbed into paper discs (diameter 6mm, Roshi Kaisha., Tokyo, Japan), followed by drying. The solvent was then volatilized. The paper disc absorbed each test substance was placed in close contact with the plate medium on which the strain was smeared, and cultured to compare the antibacterial activity by measuring the clear zone (mm) generated around the disc. The results are shown in Table 1 below. was

strains Size of clear zone (diameter, mm) PGC-1 PGC-2 PGC-3 PGC-4 Propionibacterium acnes 5.8±0.6 5.4±0.9 11.8±1.3 14.6±1.6

As shown in Table 1, as a result of analyzing the antibacterial activity of acne-causing bacteria, it was confirmed that the clear zone of the sample (PGC-4) according to Example 1 was the largest at 14.6 ± 1.6 mm, followed by the sample according to Comparative Example 3 ( PGC-3), the sample according to Comparative Example 2 (PGC-2), and the sample according to Comparative Example 1 (PGC-1), respectively, in the order of 11.8 ± 0.6 mm, 5.4 ± 0.9 mm, and 5.8 ± 1.3 mm, respectively. showed up

<Experimental Example 3> Safety and inflammation testing

(3-1) Raw 264.7 cell culture

Mouse macrophage cell line, RAW 264.7 cell, was cultured in 100 IU/mL penicillin, 100 IU/mL streptomycin, and 10% fetal bovine serum DMEM (Dulbecco's modified Eagle's medium) at 37 °C in a 5% carbon dioxide incubator.

(2) Cytotoxicity evaluation

MTS analysis was performed to confirm the cytotoxicity of the samples obtained in Example 1 and the samples (PGC-1 to 4) obtained in Comparative Examples 1 to 3.

MTS assay, a cytotoxicity test, was used as a safety test for the test substances (PGC-1~4). Quantification was determined by modifying the Mosmann method.

Specifically, RAW 264.7 cells were dispensed at 1Х10 ⁴ cell/ml and incubated for 24 hours, then the test substance (PGC-1 to 4) was diluted to a concentration of 100 to 1,000 μg/ml, added, and cultured for another 24 hours. Then, 20 μl of the MTS reagent was added and incubated for 2 hours, and the absorbance was measured at 570 nm using a microplate reader, and the cell viability was calculated by the formula below, and the results are shown in FIG. 2 below.

Cell viability (%) = [(Exp. - Blank)/Control] Х 100

(Exp: absorbance of extract containing cells, Blank: absorbance of extract without cells, Control: absorbance of distilled water containing cells)

As shown in FIG. 2 below, as a result of the cytotoxicity of the test substances (PGC-1 to 4) in RAW 264.7 cells, it was confirmed that the cell viability was 95% or more at all concentrations. Therefore, it was confirmed that the test substances (PGC-1 to 4) were not cytotoxic.

(3) LPS inflammation-inducing cytokine evaluation

It was confirmed whether or not the cytokine produced was reduced by comparing the experimental substances (PGC-1 to 4) with the control group inducing the production of inflammatory cytokine (TNF-α) in RAW 246.7 cells through LPS treatment. Cytokines are signaling substances secreted when macrophage cells are activated by external antigens.

Specifically, in order to evaluate the production of inflammation-related cytokine (TNF-α) in the cell culture medium, RAW 264.7 cells were dispensed in a 96-well plate at a concentration of 1x10 ⁴ cells/well, and then incubated at 37 °C in a 5% carbon dioxide incubator for 12 Cells were completely adhered while incubated for a period of time. Thereafter, the test substances (PGC-1 to 4) at concentrations of 0, 100, 250, and 500 μg/mL were treated, and 1 μg/mL of LPS, an inflammation-inducing substance, was treated and cultured again for 24 hours. Then, TNF-α was quantified using Mouse TNF-α quantikine ELISA kit (R&D systems®, USA), and is shown in FIG. 3 below.

As shown in Figure 3, as a result of comparing the amount of TNF-α produced after culturing the control group treated with LPS in Raw cell 246.7 cells and the test substance (PGC-1 to 4) for 24 hours, the TNF-α concentration of the control group treated only with LPS was analyzed to be the highest. It was confirmed that the concentration of TNF-α decreased as the amount of the test substance (PGC-1 to 4) increased.

That is, the TNF-α concentration decreased the most at the 500 μg/ml concentration, and was confirmed as the lowest concentration at 396.4±21.2 pg/ml in the sample (PGC-4) obtained in Example 1, followed by Comparative Example 3 460.2±33.1 μg/ml, 518.2±38.7 μg/ml, respectively, in the order of the sample obtained from (PGC-3), the sample obtained from Comparative Example 2 (PGC-2), and the sample obtained from Comparative Example 1 (PGC-1). ml, 543.8±14.7 μg/ml.

<Experimental Example 4> skin damage test

An experiment was performed to confirm the erythema index and melanin index through a skin damage induction test on the samples obtained in Example 1 and the samples obtained in Comparative Examples 1 to 3.

For reference, when the skin is exposed to ultraviolet rays, the skin is damaged by erythema and edema, so the degree of skin damage can be confirmed through erythema index evaluation. The concentration of the test substance was set to 500 μg/mL for the cytotoxicity and cytokine evaluation of the cell experiment, and the inhibition of cell damage was confirmed by evaluating the erythema index of the test substance compared to the control group.

(1) Skin damage induction animal model

A 6-week-old female SKH-1 hairless mouse is purchased, supplied with sufficient water and solid food, and managed by adapting to the laboratory environment (temperature 22±2°C, humidity 55±5%, 12-hour light/dark cycle) for one week.

The skin damage model is a sunlamp emitting 302 nm wavelength, which irradiates the back of the mouse with a light intensity of 120 mJ/cm ² equally for 30 minutes at intervals of 3 times a week for 4 weeks at intervals of every other day to induce skin barrier damage. did For the control group, 100 μL of saline solution was applied after UV irradiation at intervals of once a day, 6 days a week for 4 weeks. The experimental group applied the test substances (PGC-1 to 4) after UV irradiation at 100 μL each time at intervals of once a day, 6 days a week for 4 weeks, respectively.

(2) Erythema index evaluation

The experimental group and the control group of the skin damage induction animal model obtained were measured for erythema index once a week during the experimental period with Multiprobe MPA 5, and are shown in Table 2 below.

Days Control PGC-1 PGC-2 PGC-3 PGC-4 7 291.6±23.6 189.4±21.6 189.8±11.8 172.1±13.6 158.3±12.4 14 335.6±24.0 285.6±18.3 270.9±10.6 250.1±13.0 205.3±14.6 21 354.2±26.9 268.9±19.3 252.5±18.4 209.5±15.7 185.2±11.1 28 381.3±28.7 252.9±21.7 232.4±17.8 195.7±16.2 162.1±10.2

*Control ascorbic acid

As shown in Table 2, as a result of evaluating the skin erythema index for 28 days, the erythema index of the control group to which saline was applied was analyzed to be the highest.

All of the experimental groups had lower erythema index (381.3 ± 28.7) than the control group, and among them, the erythema index of the sample (PGC-4) obtained in Example 1 was analyzed to be the lowest at 162.1 ± 10.2. The erythema indices of the sample (PGC-3), the sample obtained in Comparative Example 2 (PGC-2), and the sample obtained in Comparative Example 1 (PGC-1) were found to be 195.7±16.2, 232.4±17.8, and 252.9±21.7, respectively. From this, it was analyzed to show a tendency similar to the LPS inflammation-inducing cytokine test results confirmed in FIG. 3 below.

(3) Evaluation of melanin index

The melanin index of the experimental group and the control group of the skin damage-induced animal model was measured once a week during the experimental period with Multiprobe MPA 5. Pigmentation of the skin occurs when a large amount of melanin pigment is produced to protect the skin when overexposed to ultraviolet rays, and spots, freckles, and blemishes occur due to the aggregation of melanin pigments. By evaluating the melanin index compared to the control group, inhibition of skin damage by ultraviolet rays was confirmed and shown in Table 3 below.

Days Control PGC-1 PGC-2 PGC-3 PGC-4 7 114.2±20.3 79.1±13.2 75.6±13.2 66.1±12.0 64.1±11.0 14 126.7±23.3 108.8±19.8 90.8±18.8 82.4±17.4 75.8±14.3 21 135.9±24.6 107.3±18.7 90.4±17.5 79.8±14.5 72.6±12.9 28 144.2±26.5 95.4±17.6 88.2±16.1 79.2±13.6 71.2±11.3

*Control ascorbic acid

As shown in Table 3, as a result of evaluating the melanin index for 28 days, the melanin index of the control group to which saline was applied was analyzed to be the highest. All of the experimental groups had a lower melanin index (144.2±17.0) than the control group, and among them, the melanin index of fermented ethanol ultrasonic malaki mixed extract (PGC-4) was analyzed to be the lowest at 71.2±11.3. The melanin indices of fermented hot water ultrasonic amalaki mixed extract (PGC-3), ethanol ultrasonic amalaki mixed extract (PGC-2), and hot water ultrasonic amalaki mixed extract (PGC-1) were 79.2±13.1, 88.2±12.8, and 95.4, respectively. It was confirmed as ± 15.1, which was analyzed to be similar to the results of the LPS inflammation-inducing cytokine experiment in the cell experiment.

<Experimental Example 5> long-term weight evaluation test

In order to confirm the toxicity of the sample (PGC-4) obtained in Example 1 and the samples (PGC-1 to 3) obtained in Comparative Examples 1 to 3, the weight of the spleen and thymus of the mouse after the experiment was measured was measured. and compared. The weight was measured as the absolute weight of the organs measured as they are and the relative weight of the organs measured by the weight ratio of the body weight.

Specifically, in order to confirm the safety of the test substances (PGC-1 to 4), after the skin damage induction animal model experiment, each experimental group and control group were anesthetized with ether, and the spleen and thymus were extracted, washed with physiological saline, and dried with a filter paper. Remove. Thereafter, the absolute weight of the spleen and thymus of the experimental group and the control group and the relative weight of the spleen and thymus were obtained from the body weight, and are shown in Table 4 below.

Control PGC-1 PGC-2 PGC-3 PGC-4 Spleen Absolute weight (g) 0.12±0.04 0.13±0.04 0.11±0.04 0.12±0.03 0.12±0.103 Relative weight (%) 0.41±0.09 0.41±0.09 0.40±0.09 0.42±0.10 0.40±0.08 Thymus Absolute weight (g) 0.04±0.01 0.04±0.01 0.04±0.01 0.04±0.01 0.04±0.01 Relative weight (%) 0.14±0.03 0.15±0.02 0.16±0.02 0.14±0.04 0.15±0.04

*Control ascorbic acid

As shown in Table 4, as a result of measuring the organ weight, it was confirmed that there was no difference between the absolute weight and the relative weight of the spleen and thymus of the control group and the test materials (PGC-1 to 4).

That is, as confirmed in the MTS assay of the cell experiment, it can be seen that there is no toxicity in the experimental group in the animal experiment.

In conclusion, Amalaki ( Phyllanthus emblica ), Geranium ( Geranium ), and Googul ( Commiphora myrrha ) When the ultrasonic extract of the fermentation broth obtained by inoculating the fermented strain into the solid dried material using the active ingredient is included, the obtained composition is a radical scavenger Active, antibacterial activity against acne-causing bacteria, inflammation-inducing cytokine production reduction effect, and skin damage induction reduction effect are simultaneously expressed to improve acne, scars, pores, blemishes, pigmentation, redness, pimples, or skin diseases. It was confirmed that it is suitable for

Claims (6)

Amalaki ( Phyllanthus emblica ), Geranium ( Geranium ) and Googul ( Commiphora myrrha ) in solid phase dry matter under polar protic solvents Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus plantarum ( Lactobacillus plantarum ), lactose Bacillus bulgaricus ( Lactobacillus bulgaricus ), Enterococcus faecium ( Enterococcus faecium ), Bifidobacterium longum ( Bifidobacterium longum ) and Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) A skin health promoting composition comprising, as an active ingredient, an ultrasonic extract of a fermentation broth obtained by inoculating one or more fermented strains selected from the group consisting of: According to claim 1,
The ultrasonic extraction is extracted for 10 to 180 minutes at a frequency of 25 to 75 kHz at 20 to 70 ° C. skin health promoting composition. According to claim 1,
The polar protic solvent comprises at least one selected from water, chloroform, ethanol, methanol, ethyl acetate, nucleic acid and diethyl ether in an amount of 5 to 20 times the dry weight of the solid phase skin health promoting composition. According to claim 1,
The amalaki ( Phyllanthus emblica ), geranium ( Geranium ), and guggul ( Commiphora myrrha ) are amalaki (a): geranium (b): guggul (c) which satisfies the weight ratio of 1: 0.75 to 4: 0.75 to 4 Skin health promoting composition. According to claim 1,
The ultrasonic extract is a skin health promoting composition extracted for 2 to 3 hours at 60 to 70 ° C. under reflux. According to claims 1 to 5,
The skin health is a skin health promoting composition that provides an effect of improving acne and scars, pores, blemishes, pigmentation, redness, pimples or skin diseases.

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