KR20230028363A - 개변된 유전 암호표를 갖는 번역계 - Google Patents

개변된 유전 암호표를 갖는 번역계 Download PDF

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Publication number
KR20230028363A
KR20230028363A KR1020237000660A KR20237000660A KR20230028363A KR 20230028363 A KR20230028363 A KR 20230028363A KR 1020237000660 A KR1020237000660 A KR 1020237000660A KR 20237000660 A KR20237000660 A KR 20237000660A KR 20230028363 A KR20230028363 A KR 20230028363A
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South Korea
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trna
codon
amino acid
anticodon
translation system
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Korean (ko)
Inventor
가오리 니시무라
다카아키 다니구치
쇼지로 시노하라
마나 가고타니
미키 미사이즈
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추가이 세이야쿠 가부시키가이샤
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Publication of KR20230028363A publication Critical patent/KR20230028363A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020237000660A 2020-06-25 2021-06-25 개변된 유전 암호표를 갖는 번역계 Pending KR20230028363A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2020-109576 2020-06-25
JP2020109576 2020-06-25
PCT/JP2021/024075 WO2021261577A1 (ja) 2020-06-25 2021-06-25 改変された遺伝暗号表を有する翻訳系

Publications (1)

Publication Number Publication Date
KR20230028363A true KR20230028363A (ko) 2023-02-28

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ID=79281398

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237000660A Pending KR20230028363A (ko) 2020-06-25 2021-06-25 개변된 유전 암호표를 갖는 번역계

Country Status (7)

Country Link
US (1) US20240052340A1 (https=)
EP (1) EP4166675A4 (https=)
JP (1) JPWO2021261577A1 (https=)
KR (1) KR20230028363A (https=)
CN (1) CN115667539A (https=)
TW (1) TW202208623A (https=)
WO (1) WO2021261577A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
KR20250007021A (ko) 2017-06-09 2025-01-13 추가이 세이야쿠 가부시키가이샤 N-치환 아미노산을 포함하는 펩타이드의 합성 방법
EP3878836B1 (en) 2018-11-07 2025-07-23 Chugai Seiyaku Kabushiki Kaisha O-substituted serine derivative production method
EP3939962A4 (en) 2019-03-15 2022-12-28 Chugai Seiyaku Kabushiki Kaisha PROCESS FOR PREPARING AN AROMATIC AMINO ACID DERIVATIVE
BR112022007535A2 (pt) 2019-11-07 2022-07-12 Chugai Pharmaceutical Co Ltd Composto peptídico cíclico tendo ação inibitória de kras
JP7823181B2 (ja) 2022-05-06 2026-03-03 中外製薬株式会社 Ｈｒａｓおよびｎｒａｓに対して選択的なｋｒａｓ阻害作用を有する環状化合物
JPWO2024128195A1 (https=) * 2022-12-12 2024-06-20

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016154675A1 (en) 2015-03-27 2016-10-06 The University Of Queensland Platform for non-natural amino acid incorporation into proteins

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
WO2006135096A1 (ja) 2005-06-14 2006-12-21 Gifu University 部位特異的にタンパク質にチロシンアナログを導入する方法
WO2007061136A1 (ja) 2005-11-24 2007-05-31 Riken 非天然型アミノ酸を組み込んだタンパク質の製造方法
WO2007066627A1 (ja) 2005-12-06 2007-06-14 The University Of Tokyo 多目的アシル化触媒とその用途
AU2007224019A1 (en) 2006-03-03 2007-09-13 California Institute Of Technology Site-specific incorporation of amino acids into molecules
US9274130B2 (en) 2006-05-31 2016-03-01 Lpath, Inc. Prevention and treatment of pain using antibodies to lysophosphatidic acid
WO2008001947A1 (en) 2006-06-28 2008-01-03 Riken MUTANT SepRS, AND METHOD FOR SITE-SPECIFIC INTRODUCTION OF PHOSPHOSERINE INTO PROTEIN BY USING THE SAME
US20120077224A1 (en) 2009-06-05 2012-03-29 The Salk Institute For Biological Studies unnatural amino acid incorporation in eukaryotic cells
JP5725467B2 (ja) 2010-08-27 2015-05-27 国立大学法人 東京大学 新規人工翻訳合成系
JP5818237B2 (ja) 2010-09-09 2015-11-18 国立大学法人 東京大学 Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法
EP2647720B1 (en) 2010-12-03 2019-06-19 The University of Tokyo Peptide library production method, peptide library, and screening method
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
JP6754997B2 (ja) 2013-08-26 2020-09-16 国立大学法人 東京大学 大環状ペプチド、その製造方法、及び大環状ペプチドライブラリを用いるスクリーニング方法
WO2015120287A2 (en) 2014-02-06 2015-08-13 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2018052002A1 (ja) 2016-09-13 2018-03-22 第一三共株式会社 Ｔｈｒｏｍｂｏｓｐｏｎｄｉｎ １結合ペプチド
JP7187323B2 (ja) * 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
CN110869544B (zh) 2017-06-09 2024-03-08 中外制药株式会社 膜透过性高的环状肽化合物及包含其的文库
WO2019193416A1 (en) * 2018-04-03 2019-10-10 The University Of Queensland Oligonucleotide-mediated sense codon reassignment

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016154675A1 (en) 2015-03-27 2016-10-06 The University Of Queensland Platform for non-natural amino acid incorporation into proteins

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Cui et al., J Am Chem Soc. 2015; 137(13): 4404-4413.
Frenkel-Morgenstern et al., Mol Syst Biol. 2012; 8: 572.
Iwane et al., Nat Chem. 2016; 8(4): 317-325.
Mukai et al., Nucleic Acids Res. 2015; 43(16): 8111-8122.
Shimizu et al., Nat Biotechnol. 2001; 19(8): 751-755.

Also Published As

Publication number Publication date
EP4166675A1 (en) 2023-04-19
CN115667539A (zh) 2023-01-31
US20240052340A1 (en) 2024-02-15
EP4166675A4 (en) 2025-06-11
JPWO2021261577A1 (https=) 2021-12-30
TW202208623A (zh) 2022-03-01
WO2021261577A1 (ja) 2021-12-30

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