CN115667539A - 具有改变的遗传密码表的翻译系统 - Google Patents

具有改变的遗传密码表的翻译系统 Download PDF

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Publication number
CN115667539A
CN115667539A CN202180036193.4A CN202180036193A CN115667539A CN 115667539 A CN115667539 A CN 115667539A CN 202180036193 A CN202180036193 A CN 202180036193A CN 115667539 A CN115667539 A CN 115667539A
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China
Prior art keywords
trna
codon
translation system
amino acid
represented
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CN202180036193.4A
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English (en)
Chinese (zh)
Inventor
西村香绪梨
谷口贵昭
筱原正次郎
笼谷万奈
美细津美树
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Chugai Pharmaceutical Co Ltd
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Chugai Pharmaceutical Co Ltd
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Publication of CN115667539A publication Critical patent/CN115667539A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202180036193.4A 2020-06-25 2021-06-25 具有改变的遗传密码表的翻译系统 Pending CN115667539A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-109576 2020-06-25
JP2020109576 2020-06-25
PCT/JP2021/024075 WO2021261577A1 (ja) 2020-06-25 2021-06-25 改変された遺伝暗号表を有する翻訳系

Publications (1)

Publication Number Publication Date
CN115667539A true CN115667539A (zh) 2023-01-31

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CN202180036193.4A Pending CN115667539A (zh) 2020-06-25 2021-06-25 具有改变的遗传密码表的翻译系统

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Country Link
US (1) US20240052340A1 (https=)
EP (1) EP4166675A4 (https=)
JP (1) JPWO2021261577A1 (https=)
KR (1) KR20230028363A (https=)
CN (1) CN115667539A (https=)
TW (1) TW202208623A (https=)
WO (1) WO2021261577A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
EP4166664A1 (en) 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
JP7187323B2 (ja) 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
KR20250007021A (ko) 2017-06-09 2025-01-13 추가이 세이야쿠 가부시키가이샤 N-치환 아미노산을 포함하는 펩타이드의 합성 방법
EP3878836B1 (en) 2018-11-07 2025-07-23 Chugai Seiyaku Kabushiki Kaisha O-substituted serine derivative production method
EP3939962A4 (en) 2019-03-15 2022-12-28 Chugai Seiyaku Kabushiki Kaisha PROCESS FOR PREPARING AN AROMATIC AMINO ACID DERIVATIVE
BR112022007535A2 (pt) 2019-11-07 2022-07-12 Chugai Pharmaceutical Co Ltd Composto peptídico cíclico tendo ação inibitória de kras
JP7823181B2 (ja) 2022-05-06 2026-03-03 中外製薬株式会社 Ｈｒａｓおよびｎｒａｓに対して選択的なｋｒａｓ阻害作用を有する環状化合物
JPWO2024128195A1 (https=) * 2022-12-12 2024-06-20

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103189522A (zh) * 2010-08-27 2013-07-03 国立大学法人东京大学 新型人工翻译合成系统
JP2018509172A (ja) * 2015-03-27 2018-04-05 ザ ユニバーシティ オブ クィーンズランド タンパク質への非天然アミノ酸組込みのためのプラットフォーム

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WO2006135096A1 (ja) 2005-06-14 2006-12-21 Gifu University 部位特異的にタンパク質にチロシンアナログを導入する方法
WO2007061136A1 (ja) 2005-11-24 2007-05-31 Riken 非天然型アミノ酸を組み込んだタンパク質の製造方法
WO2007066627A1 (ja) 2005-12-06 2007-06-14 The University Of Tokyo 多目的アシル化触媒とその用途
AU2007224019A1 (en) 2006-03-03 2007-09-13 California Institute Of Technology Site-specific incorporation of amino acids into molecules
US9274130B2 (en) 2006-05-31 2016-03-01 Lpath, Inc. Prevention and treatment of pain using antibodies to lysophosphatidic acid
WO2008001947A1 (en) 2006-06-28 2008-01-03 Riken MUTANT SepRS, AND METHOD FOR SITE-SPECIFIC INTRODUCTION OF PHOSPHOSERINE INTO PROTEIN BY USING THE SAME
US20120077224A1 (en) 2009-06-05 2012-03-29 The Salk Institute For Biological Studies unnatural amino acid incorporation in eukaryotic cells
JP5818237B2 (ja) 2010-09-09 2015-11-18 国立大学法人 東京大学 Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法
EP2647720B1 (en) 2010-12-03 2019-06-19 The University of Tokyo Peptide library production method, peptide library, and screening method
TW202446781A (zh) 2011-12-28 2024-12-01 日商中外製藥股份有限公司 胜肽化合物之製造方法
JP6754997B2 (ja) 2013-08-26 2020-09-16 国立大学法人 東京大学 大環状ペプチド、その製造方法、及び大環状ペプチドライブラリを用いるスクリーニング方法
WO2015120287A2 (en) 2014-02-06 2015-08-13 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2018052002A1 (ja) 2016-09-13 2018-03-22 第一三共株式会社 Ｔｈｒｏｍｂｏｓｐｏｎｄｉｎ １結合ペプチド
JP7187323B2 (ja) * 2017-01-31 2022-12-12 中外製薬株式会社 無細胞翻訳系におけるペプチドの合成方法
CN110869544B (zh) 2017-06-09 2024-03-08 中外制药株式会社 膜透过性高的环状肽化合物及包含其的文库
WO2019193416A1 (en) * 2018-04-03 2019-10-10 The University Of Queensland Oligonucleotide-mediated sense codon reassignment

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN103189522A (zh) * 2010-08-27 2013-07-03 国立大学法人东京大学 新型人工翻译合成系统
JP2018509172A (ja) * 2015-03-27 2018-04-05 ザ ユニバーシティ オブ クィーンズランド タンパク質への非天然アミノ酸組込みのためのプラットフォーム

Also Published As

Publication number Publication date
EP4166675A1 (en) 2023-04-19
US20240052340A1 (en) 2024-02-15
KR20230028363A (ko) 2023-02-28
EP4166675A4 (en) 2025-06-11
JPWO2021261577A1 (https=) 2021-12-30
TW202208623A (zh) 2022-03-01
WO2021261577A1 (ja) 2021-12-30

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