KR20230015533A - Abrasive composition for toothpaste comprising enzyme treated pear ground and toothpaste comprising the same - Google Patents

Abstract

The present invention relates to a method for preparing an abrasive composition for toothpaste and a toothpaste composition prepared thereby, wherein the method comprises the steps of: (a) thermally treating crushed pear liquid and filtering the same to remove the pear juice and drying a by-product to obtain ground pear powder; (b) grinding the ground pear powder to screen the ground pear powder such that the ground pear powder has a size where the ground pear powder passes through a 30-mesh net and can't pass through a 60-mesh net, thereby obtaining the screened ground pear powder; (c) making a reaction of the screened ground pear powder with alcohol to obtain a precipitate; and (d) treating the precipitate with a composite enzyme including cellulase and hemicellulose and removing a supernatant from the resulting product subjected to treatment with the composite enzyme to dry the precipitate with hot air. Accordingly, the toothpaste composition has remarkably excellent effects in proper abrasion of teeth, prevention and removal of plaque and tartar, and inhibition of gingivitis, compared to conventional commercial toothpaste or toothpaste where a ground pear ingredient is simply introduced and a natural material is used so that the toothpaste composition is non-toxic to a human body.

Description

A toothpaste abrasive composition comprising a pear enzyme-treated material and a toothpaste composition comprising the same

The present invention relates to a toothpaste abrasive composition comprising an enzyme-treated basil and a toothpaste composition comprising the same, and more particularly, to a human toothpaste abrasive comprising an enzyme-treated basil containing stone cells and a human toothpaste comprising the same It's about the composition.

Recently, the plantation area in Korea has expanded in a very short period of time due to the conversion to other crops with relatively low economic feasibility after import liberalization, and has become an important fruit species that accounts for more than 16% of the total fruit tree cultivation area. Oriental pears have recently been exported to Europe, the largest import and export country of pears, and the characteristics of oriental pears, which have a lot of moisture in the flesh and can be consumed raw, have begun to be known. In particular, pears with a lot of hard stone cells composed of lignin and cellulose are known to be poor because of the strong texture of the fruit. In Western pears, many studies on stone cells have been conducted, such as the composition of stone cells, the time of formation, and the density of stone cells in the pulp. However, studies on southern oriental pears, which are mainly cultivated in Korea, are insufficient.

Stone cells are also called reinforcing cells or sclerenchyma cells, and have shorter, thicker, irregular, and very hard secondary cell walls than fibrous cells. Stone cells are found in fruit tissues, especially in the pulp of the genus Pyrus. Stone cells are a type of lignification in which lignin is deposited on cell walls in tissues, and it is known that they serve as a framework for fixing cell walls so that they do not change in shape by strengthening cell walls.

The main components of stone cells in pear fruits were studied by Ranadive and Haard in western pears a long time ago. It is known to contain 18 to 35% of lignin, which is not found in the primary cell wall. In addition, the main units of lignin are vanillin and syringaldehyde.

On the other hand, toothpaste is a kind of tooth cleaner for preventing or removing food remnants, bacterial film, plaque, calculus, etc. attached to teeth, and may contain ingredients that prevent tooth decay or bad breath by delivering ingredients effective in preventing tooth decay. Toothpaste includes a liquid type with low viscosity, a paste type with high viscosity, and a powder type, but the paste type is mainly used. On the other hand, the components of toothpaste are abrasives that physically remove debris attached to teeth, surfactants that help effectively remove debris that is firmly attached to teeth, excipients that help maintain water and shape to have appropriate viscosity, A moisturizer that prevents hardening is the main component, and additives such as fragrance, sweetener, preservative, colorant, etc. may be included so that there is no objection during brushing.

The role of the toothpaste abrasive is to physically remove debris on the tooth surface, but the abrasive degree of abrasive is important because it can abrade tooth enamel or dentin. Representative abrasives include monocalcium hydrogen phosphate, precipitated calcium carbonate, silica, and insoluble sodium metaphosphate. These abrasives have a problem in that tooth enamel may be worn due to their hardness being higher than that of apatite carbonate, a component of tooth enamel.

On the other hand, in order to solve the above problems, toothpaste compositions containing natural products have been developed. Conventionally developed oral compositions such as toothpaste have been disclosed that include plant extracts such as green tea extract, moth extract, horsetail extract, and myrrh extract in walnut shell powder, which are effective in removing plaque and preventing gum disease. , In the case of walnut shell powder particles, the surface is very rough, so the enamel of teeth can be worn, and since most of the walnut shell powder is imported, there is a problem in that expensive costs are required when used for polishing purposes.

In addition, a toothpaste composition comprising vegetable abrasives obtained by freeze-drying a component including fibers of leaves, stems, and fruit skins of plants and then pulverizing the fiber powder with a starch solution or polymer and coating is disclosed, but such Since the polishing effect cannot be greatly improved with only vegetable abrasives, there is a problem in that abrasives composed of chemical components such as silica, potassium carbonate, sodium bicarbonate, calcium monohydrogen phosphate, and alumina must be included.

Korean Patent Publication No. 10-2010-003429 Korean Patent Publication No. 10-2004-0054317

The object of the present invention is that the effects such as proper polishing of teeth, prevention and removal of plaque and calculus, and inhibition of gingivitis are significantly superior to conventional commercial toothpaste or toothpaste in which simple ingredients are introduced, and are harmless to the human body by using natural materials. It is to provide a toothpaste abrasive containing an enzyme-treated product of pear containing stucco cells and a toothpaste composition containing the same.

Another object of the present invention is a functional component using pear peel without using complicated processes or harmful chemicals, screening pear material by particle size, and performing alcohol treatment and enzyme treatment to achieve tooth polishing effect, gingivitis, and plaque. And a method for producing a toothpaste abrasive containing an enzyme-treated product of pear bark containing stone cells that is significantly superior to conventional commercial toothpaste or toothpaste in which the tartar inhibitor component is simply introduced, and a method for producing a toothpaste composition containing the same. there is

According to one aspect of the present invention,

(a) heat-treating the pear pulverized solution, filtering to remove the pear juice, and drying the by-product to obtain pear pear powder;

(B) pulverizing the pear powder and then passing through a 30 mesh mesh and screening to a size that does not pass through a 60 mesh mesh to obtain a sorted pear shell powder;

(c) reacting the selected pear powder with alcohol to obtain a precipitate; and

(d) treating the precipitate with a complex enzyme containing cellulase and hemicellulase, removing the supernatant from the complex enzyme-treated product and drying the precipitate with hot air; A method for preparing an abrasive composition is provided.

In step (a), the high-pressure heat treatment may be performed at 100 to 140 °C.

In step (c), the alcohol may be a lower alcohol having 1 to 4 carbon atoms.

The alcohol may be an aqueous solution of ethanol at a concentration of 40 to 50%.

In step (d), the complex enzyme may be added in an amount of 0.01 to 0.15 wt% of solids.

In step (d), the complex enzyme may be used by mixing cellulase and hemicellulase at a weight ratio of 1:0.5 to 0.5:1.

In step (d), the enzymatic treatment may be performed at 35 to 40° C. for 8 to 20 hours.

In step (d), the hot air drying may be performed at 50 to 70° C. for 10 to 20 hours.

An abrasive composition for human use prepared according to the method for preparing an abrasive composition for human use is provided.

The abrasive composition for human use may have a total dietary fiber content of 75 to 85% (w/v).

The human abrasive composition may have an insoluble dietary fiber content of 65 to 80% (w/v).

The stone cell content may be 85 to 95% based on the total weight of the insoluble dietary fiber.

The human abrasive composition may have a water absorption capacity of 1.2 to 2 ml/g based on the passage of 1 to 24 hours.

According to another aspect of the present invention,

A human toothpaste composition comprising the human abrasive composition prepared according to the method for preparing the human abrasive composition is provided.

The human toothpaste composition may have a sugar content of 30 to 34 brix.

The human abrasive composition may be included in a state of additional grinding to 500 to 800 mesh.

The human toothpaste composition may include 1 to 7 wt% of the human abrasive composition based on the total weight.

The human dentifrice composition may be for removing or inhibiting gingivitis, plaque or calculus.

The toothpaste abrasive material containing the enzymatically treated pear shell containing stone cells and the toothpaste composition containing the same of the present invention have effects such as proper polishing of teeth, prevention and removal of plaque and calculus, and inhibition of gingivitis, such as conventional commercial toothpaste or pear shell It is remarkably superior to toothpaste that simply introduces ingredients, and has an effect that is harmless to the human body by using natural ingredients.

A method for producing a toothpaste abrasive containing an enzyme-treated product of pears containing stone cells and a method for producing a toothpaste composition containing the same of the present invention is to prepare functional ingredients using pears without using complex processes or harmful chemicals, By simply selecting materials by particle size and performing alcohol treatment and enzyme treatment, the tooth polishing effect, gingivitis, plaque and calculus inhibitory effect are significantly superior to conventional commercial toothpaste or toothpaste simply introducing pear extract components.

1 is a photograph of functional toothpastes for humans according to Examples 1 to 3 and Comparative Examples 1 and 2.
2 is a stereomicrograph after staining of a pear shell powder sample according to Experimental Example 1.
Figure 3 is a standard curve according to the dyed photograph and color difference value according to the stone cell concentration for examining stone cell purity in Experimental Example 1.
4 shows a polishing process using beagle teeth according to Experimental Example 4.
5 is a 3D-OP surface shape photograph of a tooth before and after using the toothpaste according to Experimental Example 4;
6 is a photograph of performing scaling and brushing according to Experimental Example 5.
7 is a photograph of fecal evaluation criteria according to Experimental Example 5.
8 is a result of measuring the gingival index (GI), plaque index (PI), and calculus index (CI) of an untreated control group without brushing according to Experimental Example 5.
Figure 9 is a gingival index (GI), plaque index (PI), calculus index (CI) in the teeth of experimental beagles treated with the toothpaste containing walnut shells according to Experimental Example 5 and the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3 ) is the result of measuring
10 is a photograph showing the dental condition of a beagle after 4 weeks of brushing using the untreated, walnut shell-containing toothpaste according to Experimental Example 5 and the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3.
11 is a fecal evaluation result according to Experimental Example 5.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, it should be understood that this is not intended to limit the present invention to specific embodiments, and includes all transformations, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

Terms used in this application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Hereinafter, a method for producing a toothpaste abrasive containing the enzyme-treated pear shell of the present invention will be described.

First, the pear pulverized liquid is heat-treated, filtered to remove pear juice, and the by-product is dried to obtain pear pear powder (step a).

The heat treatment is preferably performed at 100 to 140 °C, more preferably at 110 to 130 °C, and even more preferably at 115 to 125 °C.

The filtration is preferably performed 2 to 3 times using a non-woven fabric to remove pear juice.

Thereafter, the pear powder is pulverized and then passed through a 30 mesh mesh and screened to a size that does not pass through a 60 mesh mesh to obtain a sorted pear shell powder (step b).

The selected pear powder is preferably selected to a size that passes through a 30 mesh mesh and does not pass through a 60 mesh mesh, more preferably a size that passes through a 30 mesh mesh and does not pass through a 50 mesh mesh, even more preferably Preferably, it can be used by selecting a size that passes through a 30 mesh network and does not pass through a 50 mesh network. In the case of selecting the size of such particles, the density of stone cells can be increased.

In the present invention, the mesh unit was based on KS A 5101 (a unit representing particle size by a standard sieve), and the mesh and micrometer (μm) unit conversion table according to this is shown in Table 1 below.

Next, the selected pear powder is reacted with alcohol to obtain a precipitate (step c).

In step (c), the alcohol is preferably a lower alcohol having 1 to 4 carbon atoms, more preferably a 40 to 50% aqueous ethanol solution, and even more preferably a 43 to 47% concentration It may be an aqueous solution of ethanol.

The reaction with the alcohol is preferably carried out while stirring at a speed of 100 to 140 rpm at 20 to 30 ° C., and more preferably reacted while stirring at a speed of 110 to 130 rpm at 23 to 27 ° C. to accelerate the reaction.

Finally, after treating the precipitate with a complex enzyme containing cellulase and hemicellulase, the supernatant is removed from the resultant product treated with the complex enzyme, and the precipitate is dried with hot air (step d).

The complex enzyme is preferably a mixture of cellulase and hemicellulase at a weight ratio of 1:0.5 to 0.5:1, more preferably 1:0.7 to 0.7:1, and even more preferably 1 : 0.8 to 0.8: 1 may be mixed. In the case of using the complex enzyme mixed in the above range, the polishing effect, gingivitis, plaque and calculus inhibitory effect can be improved.

The reaction with the complex enzyme may be performed by adding a buffer solution.

As the buffer solution, sodium acetate, sodium phosphate, 2-ethanesulfonic acid (PIPES), water, etc. may be used, and sodium acetate may be preferably used.

The reaction with the complex enzyme is preferably carried out at 35 to 40° C. for 10 to 20 hours. In this case, the activity of the enzyme is the best, and when the abrasive, which is an enzyme reaction product, is used in toothpaste, the abrasive effect, gingivitis, plaque and calculus inhibitory effect can be increased.

The obtained enzyme-treated material is centrifuged to obtain a precipitate, which can be used as a functional abrasive composition for human toothpaste.

The present invention provides a toothpaste abrasive composition prepared according to the method for preparing the toothpaste abrasive composition.

The toothpaste abrasive composition may have a total dietary fiber content of 75 to 85% (w/v). Here, total dietary fiber means the content including insoluble dietary fiber and soluble dietary fiber.

In addition, the toothpaste abrasive composition may have an insoluble dietary fiber content of 65 to 80% (w/v), preferably 70 to 75% (w/v). Here, the water-soluble dietary fiber content is the total dietary fiber content minus the insoluble dietary fiber content, and since water-soluble dietary fiber has high water absorption, it may reduce formulation stability when used as a toothpaste component, so a formulation with a low water-soluble dietary fiber content It can be advantageous to improve stability.

In addition, the content of stone cells in the total amount of insoluble dietary fiber may be 85 to 95%, preferably 90 to 93%. Here, stone cells can be defined as components containing lignocellulose and ligne. When the stone cell content is within the above range, polishing performance can be remarkably improved when used as an abrasive composition for toothpaste.

The high stone cell content in the total amount of insoluble dietary fiber in the toothpaste abrasive composition of the present invention is because cellulose, hemicellulose, etc. other than stone cells are removed by the complex enzyme treatment.

The toothpaste abrasive composition may have a water absorption capacity of 1.2 to 2 ml/g, preferably 1.4 to 1.6 ml/g, based on the lapse of 1 to 24 hours. This low water absorption capacity seems to be due to the high purity of stone cells, and there is an effect of preventing formulation stability deterioration due to water absorption during toothpaste manufacturing.

The present invention provides a toothpaste composition comprising the toothpaste abrasive.

The toothpaste composition preferably has a sugar content of 30 to 34 brix, more preferably 31 to 33 brix. If it is less than 30 brix, the degree of acceptability may decrease, and if it exceeds 34 brix, the effect of preventing caries may decrease.

The toothpaste abrasive is preferably applied to toothpaste by first grinding to 100 to 200 mesh and finely grinding to 500 to 800 mesh. Grinding with a mesh of 800 mesh or more may lower the abrasive power, and if the mesh is less than 500 mesh, the abrasive power is high and may damage gums or teeth.

The dentifrice composition preferably contains 1 to 7 wt% of the toothpaste abrasive based on the total weight, more preferably 2 to 6 wt%. If it is included in less than 1wt%, the abrasive effect, gingivitis, plaque and calculus inhibitory effect may be reduced, and if it exceeds 7wt%, the tooth surface may be damaged or the gums may be irritated or damaged.

The dentifrice composition may be selected from cream, powder, paste, gel and liquid types.

The dentifrice composition can be used for removing or inhibiting gingivitis, plaque or calculus.

The toothpaste composition of the present invention may include a cleaning agent together with the toothpaste abrasive.

Conventional surfactants may be used as the cleaning agent. These detergents prevent foreign substances separated from the tooth surface during brushing from adhering to the tooth surface again, and function to ensure that they are completely discharged from the mouth during brushing. These detergents include alkyl sulfates, olefin sulfonates, monoglyceride sulfonates, etc. Anionic surfactants such as polyoxyethylene sorbitan fatty acid ester, polyoxyethylene castor oil, glycerin fatty acid ester, nonionic surfactants such as alkylolamide, and amphoteric surfactants such as Pluronic F-108. can

All of these detergents are listed by way of example, and in addition to these, various kinds of detergents having surface activity can be used as long as they are not harmful to the human body.

In addition, an excipient may be used to increase the weight or form a paste, etc., and an excipient means an orally acceptable carrier.

The toothpaste composition may further include xylitol, a fluorine compound, or a caries preventing component composed of a mixture of two or more thereof in an amount of 0.1 to 5% by weight based on the total weight of the toothpaste composition. The xylitol is obtained from a natural substance such as a birch tree extract, and is known to be effective in preventing tooth decay by suppressing the propagation of bacteria while having a sweet taste but not containing sugar. The xylitol can also be used as a sweetener. Fluorine compounds have also been known to be effective in preventing tooth decay for a long time, and are known to those skilled in the art to easily purchase and use them.

In addition, the toothpaste composition of the present invention may further include other wetting agents, binders, foaming agents, sweeteners, flavoring agents, coloring agents, or auxiliary components consisting of a mixture of two or more of them in an amount of 0.1 to 50% by weight based on the total weight of the toothpaste composition. there is. These wetting agents, foaming agents, sweetening agents, flavoring agents, and coloring agents are also things that can be understood as known enough to be easily purchased and used by those skilled in the art of toothpaste manufacturing.

In addition, titanium dioxide or the like may be used to improve whitening. If the auxiliary components are included in less than 0.1% by weight based on the total weight of the toothpaste composition, there may be a problem in that it is difficult to form an appropriate toothpaste by these auxiliary components, and conversely, even if it exceeds 50% by weight, other There may be a problem that causes a lack of content of ingredients.

On the other hand, a humectant is an essential base component for making toothpaste formulations, and prevents toothpaste from drying and solidifying when exposed to air, provides gloss to the surface of toothpaste, and also serves to give a sweetening effect when brushing teeth depending on the type. In addition, the binder serves to prevent separation between the solid powder component and the liquid component. Any type of water-soluble polymer can be used, and commonly used ingredients include sodium carboxymethylcellulose synthesized from cellulose of trees, carrageenan extracted from seaweed, and xanthan gum obtained from metabolism of microorganisms. . In addition, the foaming agent enhances the feeling of use of the product, helps in cleaning, and quickly disperses and penetrates other active ingredients, and reduces interfacial tension to easily remove foreign substances in the oral cavity. As the foaming agent, sodium lauryl sulfate, which is an anionic surfactant, polyoxyethylene-polyoxypropylene copolymer (poloxamer), polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, and the like may be used.

In addition to the above components, spices, sweeteners, colorings, etc. may be used to improve the feeling of use, and since spices used for this purpose are common, it is essential to use edible spices. Saccharin sodium and stevioside are used as the sweetening agent, and food coloring is preferably used as the colorant.

The present invention provides a method for producing a toothpaste composition including a method for producing a toothpaste abrasive.

Examples of the present invention will be described below, but the scope of the present invention is not limited thereto.

[Example]

Preparation Example 1: Preparation of pear shell powder

100 g of the pear pulverized liquid was heated at 120 ° C. for 3 hours under high pressure and filtered twice through a non-woven fabric to prepare pear juice, and then pear pear powder, a remaining by-product, was obtained, and the lightness was measured as 60.2 - 65.4.

Preparation Example 2: Selected pear shell powder

After pulverizing the pear powder prepared in Preparation Example 1 with a blender, only the 30 to 60 mesh size in which stone cells are dense was selected to prepare a selected pear powder.

Preparation Example 3: Enzyme-treated abrasive powder

The selected pear powder prepared according to Preparation Example 2 was subjected to ethanol treatment and enzyme treatment.

Specifically, in the ethanol treatment, 10 g of the sample was reacted at 120 rpm at 25 ° C. for 1 hour in a 45% ethanol aqueous solution contained in a 1 L Erlenmeyer flask to dissolve stone cells and dietary fiber. Thereafter, centrifugation was performed at 8,000 rpm for 10 minutes, and the supernatant was removed at 4°C. Thereafter, it was washed twice with water, and residual ethanol components were removed by centrifugation.

Next, the ethanol-treated selected pear powder was subjected to enzyme treatment. Enzyme treatment is performed by adding 0.1% by weight of a complex enzyme in which cellulase and hemicellulase are mixed at a weight ratio of 1:1, and then using sodium acetate buffer (0.01M, pH 5.0). ) was added in 450 ml and reacted for 15 hours at a speed of 80 rpm at 37 ° C. After centrifugation, the supernatant was completely removed and dried at 60 ° C. for 15 hours.

Example 1: Preparation of functional toothpaste containing pear shell powder

With the composition shown in Table 2 below, the pear shell powder of Preparation Example 1 was first grinded to 160 mesh, and then finely ground to 800 mesh using an ACM (air classified machines) device, and added as a functional component to toothpaste, and the functional component is a commercial toothpaste. Instead of 5 wt% of cellulose gum, 5 wt% of pear shell powder was included.

Example 2: Preparation of functional toothpaste containing selected pear powder

A functional toothpaste was prepared under the same conditions as in Example 1, except that the selected pear powder of Preparation Example 2 was included instead of the pear powder of Preparation Example 1.

Example 3: Preparation of functional toothpaste containing enzyme-treated abrasive powder

A functional toothpaste was prepared under the same conditions as in Example 1, except that the enzyme-treated abrasive powder of Preparation Example 3 was included instead of the pear shell powder of Preparation Example 1.

Comparative Example 1: Commercial general toothpaste

A commercial toothpaste was prepared, and a toothpaste having the same components as the toothpaste of Example 1 was prepared, except that the functional component was not included.

Comparative Example 2: Commercial Walnut Shell Toothpaste

A commercial toothpaste was prepared, and a toothpaste having the same ingredients as the toothpaste of Example 1 except for containing walnut shell powder as an abrasive component.

Blending purpose ingredient name plain toothpaste
(g/ 100g) functional toothpaste
(g/ 100g) chief ingredient Precipitated Calcium Carbonate 30 25 chief ingredient abrasive component 0 5 chief ingredient amino acid caproic acid 0.01 0.01 chief ingredient calcium monohydrogen phosphate 0.01 0.01 chief ingredient Allantoin Chlorohydroxyaluminum 0.01 0.01 mechanism disorbitol solution 33.00 33.00 mechanism propylene glycol 5.10 5.10 mechanism concentrated glycerin 8.25 8.25 mechanism Polyethylene glycol 1500 0.48 0.48 viscosity modifier Sodium carboxymethylcellulose 0.72 0.72 foaming agent sodium lauryl sulfate 2.44 2.44 weight loss xanthan gum 2.49 2.49 antiseptic sodium benzoate 0.18 0.18 sweetener green tea extract 0.01 0.01 sweetener Saccharine Sodium 0.01 0.01 sweetener xylitol 0.01 0.01 sweetener chitosan 0.01 0.01 sweetener propolis extract 0.01 0.01 sweetener aloe extract 0.01 0.01 sweetener silk powder 0.25 0.25 flavoring agent DL-menthol Appropriate amount Appropriate amount flavoring agent peppermint Appropriate amount Appropriate amount solvent Purified water Appropriate amount Appropriate amount

Examples 1 to 3, and Comparative Examples 1, 2 pictures of the functional toothpaste prepared respectively according to Figure 1 are shown.

[Experimental Example]

Experimental Example 1: stone cell purity analysis

Dietary fiber was analyzed according to the method of Prosky et al. (1988). For the content of insoluble dietary fiber (IDF), 1 g of the sample was turbid in 50 ml of phosphate buffer (pH 6.0), heat-resistant α-amylase at 95 ° C, and protease at 60 ° C. and amyloglucosidase were sequentially treated with enzymes. After filtering the enzyme-treated sample in constant weight diatomaceous earth, the filtered residue was washed with 75% ethanol, 95% ethanol and acetone, and dried for 12 hours, and the value was measured excluding protein and ash content. In addition, for soluble dietary fiber (SDF), 95% ethanol at 60 ° C was added in a ratio of 1: 4 to the filtrate filtered through diatomaceous earth, reacted for 1 hour, and then filtered again to measure the dry weight of the remaining residue. .

The total dietary fiber (TDF) content was calculated as the sum of the insoluble and soluble dietary fiber content of the residue.

Stone cells can be defined as those containing lignocellulose and lignin, and a staining method that can be distinguished from insoluble dietary fibers composed of simple cellulose or hemicellulose is required. Kwon et al. (2016). According to the method, Wiesner staining solution was composed of 2% phloroglucinol, 20% ethanol, and 20% hydrochloric acid (HCl), and was left for about 2 hours after preparation.

2% Phloroglucinol-HCl staining solution was added to the pear powder of Preparation Example 1, the selected pear powder of Preparation Example 2, and the enzymatically treated abrasive powder sample of Preparation Example 3, and then stained at room temperature for 1 hour at 100 rpm and then stained 2.5 times under a stereomicroscope. The enlarged observation results are shown in FIG. 2 . According to this, stone cells (lignocellulose + lignin) are red, and other insoluble dietary fibers, cellulose and hemicellulose, are brown, so stone cells can be distinguished.

On the other hand, for the quantification of stone cells, the redness value (a-value) was measured using a colorimeter, and the stone cell content contained in the insoluble dietary fiber powder was quantified using a standard curve to which the concentration and redness value of stone cells were applied. .

For reference, the standard curve to which the dyed photo and the redness value according to the stone cell concentration are applied is shown in FIG. 3, and the color difference value for each component content is shown in Table 3 below. Here, the stone cell concentration can be measured according to the a value.

Concentration (mg/mL) L a b c 0 2.47±0.04 0.08±0.03 -1.09±0.08 1.09±0.09 One 4.66±0.02 2.95±0.06 -1.49±0.06 3.31±0.08 3 5.35±0.06 3.92±0.06 -1.65±0.05 4.25±0.07 5 8.03±0.05 7.97±0.09 -1.15±0.04 8.05±0.09 10 6.91±0.07 10.79±0.20 -0.93±0.09 10.83±0.21 20 7.02±0.16 16.23±0.01 -0.77±0.01 16.25±0.40 30 7.57±0.06 17.93±0.05 0.50±0.05 17.93±0.31 40 6.69±0.06 18.69±0.02 0.39±0.02 18.69±0.19 50 7.49±0.06 19.36±0.05 0.59±0.05 19.37±0.27 70 7.31±0.06 19.39±0.05 0.25±0.05 19.39±0.11 100 8.51±0.02 20.00±0.02 0.73±0.02 20.01±0.01

The results measured accordingly are shown in Table 4 below.

Kinds total dietary fiber
(wt%) Insoluble fiber content
(%) Stone cell content in insoluble dietary fiber
(%) Water absorption capacity (㎖/g) 1 h 3h 24h Preparation Example 1 64.38b 16.21c 25.20c 16.55a 17.93a 19.18a Preparation Example 2 69.74b 46.41b 66.67b 2.91bc 2.80bc 3.26b Preparation Example 3 78.08a 72.21a 92.26a 1.36e 1.430d 1.54d

According to this, the enzyme-treated abrasive powder of Preparation Example 3 had a high stone cell purity of 92%, and the pear shell powder of Preparation Example 1 had a relatively low stone cell content and dietary fiber accounted for most of it at 75%. When used as a toothpaste, the water absorption capacity that causes formulation instability was calculated according to Equation 1 below.

[Equation 1]

Water absorption capacity (SC) (㎖ / g) = (V _wet -V _dry ) / W _dry

Here, V _wet represents the volume of the hydrated sample, V _dry represents the volume of the dry sample, and W _wet represents the mass of the dry sample.

According to this, the pear shell powder of Preparation Example 1 having a relatively high water-soluble dietary fiber ratio and a low stone cell content had a very high water absorption capacity of 19 ml / g, and the selected pear shell powder of Preparation Example 2 had 3.2 ml / g, Preparation Example The enzyme-treated abrasive powder of 3 showed very low water absorption even after 24 hours at 1.5 ml/g. As can be seen in the microscopic image, it seems to be due to the high purity of the stone cells that are red. If the water absorption capacity is low, it may have high formulation stability.

Experimental Example 2: Quality analysis of functional toothpaste

As the quality characteristics of the functional toothpastes prepared according to Examples 1 to 3 and Comparative Examples 1 and 2, pH, color, sugar, and acidity were measured.

Chromaticity was measured by partially modifying the method of Hur and Kim (2018). L (Lightness), a (Redness), b (yellowness), and ΔE (Total color difference) were measured using a color difference meter (Handheld color spectrophotometer 45/0 NS 800, Shenzhen 3nh Technology Co., Ltd, Shenzhen, China). . The chromaticity values of the standard whiteboard were L=96.39, a=-0.43, and b=1.33, and were calculated according to Equation 2 below.

[Equation 2]

ΔE=[(LL ₀ )2-(aa ₀ )2-(bb ₀ )2]1/2

Sugar content, pH, and acidity were measured after dissolving 1 g of the sample in distilled water at 10% (v/v). Sugar content was measured with a refractometer (Digital hand-held PAL-1, ATAGO Co., Ltd, Tokyo, Japan) for the dissolved sample. The pH of the dissolved sample was measured using a pH meter (Benchtop pH Meter, Mettler Toledo, Columbus, Ohio, USA). Acidity was titrated with 0.1 N NaOH after adding two or three drops of 1% (v/v) phenolphthalein (Junsei chemical Co., Ltd, Tokyo, Japan) to the dissolved sample. When shaking, the indicator lasted for 20 to 30 seconds in a bright red color as the end point, and after measuring the NaOH consumption, the total acidity of the sample was converted into citric acid.

The results of analyzing the quality characteristics of the functional toothpastes of Examples 1 to 3 and Comparative Examples 1 and 2 measured as described above are shown in Table 5 below.

division pH acidity
(%) Sugar content
(°brix) chromaticity L* a* b* ΔE* Comparative Example 1 7.46b 0.57bc 27.33 86.33a -0.45e 2.04e 10.03e Comparative Example 2 7.61a 0.74ab 27.67 79.43b 1.04c 6.34a 16.14d Example 1 7.13c 0.80a 32.50 65.24c 0.16d 2.55d 31.12c Example 2 7.23c 0.43c 26.67 52.55d 1.45a 4.07c 43.72b Example 3 7.42b 0.59bc 32.33 48.31e 1.33b 4.75b 47.92a

According to this, all of the toothpastes prepared according to Comparative Examples 1 and 2 and Examples 1 to 3 satisfied the pH range of 7 to 8 suitable for human toothpaste. In particular, the acidity of the toothpaste of Example 3 is similar to that of the general toothpaste of Comparative Example 1, and the sugar content is the highest at 32 ° brix, so it is expected that the preference will be good. The L value, which is brightness in the chromaticity of the sample, was 86.3 for the general toothpaste of Comparative Example 1, 79.4 for the walnut shell toothpaste of Comparative Example 2, 52.5 for the toothpaste of Example 2, 48.3 for the toothpaste of Example 3, and 48.3 for the toothpaste of Example 1. At 65.2, the toothpaste of Example 3 was the lowest. The yellowness b value was the highest in the walnut shell toothpaste of Comparative Example 2 at 6.34, and the higher the content of stone cells, the higher the yellow color. The ΔE value, which means the color difference between total brightness and saturation, is also 10.0 for the regular toothpaste of Comparative Example 1, 16.1 for the walnut shell toothpaste of Comparative Example 2, 31.1 for the toothpaste of Example 1, 43.7 for the toothpaste of Example 2, and 43.7 for the toothpaste of Example 3. Toothpaste was the highest at 47.9.

Experimental Example 3: Formulation stability analysis of functional toothpaste

For the formulation stability analysis of the functional dentifrices of Examples 1 to 3 and Comparative Examples 1 and 2, adhesion, moisture content, specific gravity, and water absorption were measured.

Gumminess was measured using a texture meter (XforceP, Zwick Roell Co., Germany), and the measurement condition was to attach a probe with a diameter of 15 mm at a start position speed of 5 mm/s, once The compression ratio was set to 70% at the time of measurement and 20% at the time of measurement when measuring twice.

Moisture content (%) was measured according to the loss on drying test method. 1 g of sample was precisely weighed, dried at 105 ° C. for 4 hours, left in a desiccator to cool, and then calculated according to Equation 3 below.

[Equation 3]

Moisture content (%) = {1-weight after drying (g)/weight of sample (g)} × 100

Density was calculated according to Equation 4 below by putting the sample into a container of known capacity and measuring its weight.

[Equation 4]

Density (g/mL) = sample weight (g)/container (mL)

Swelling Capacity is measured by mixing 1 g of sample with 20 ml of water (1:20, w/v) and then leaving it at 4 ° C. It was measured according to Equation 1 above.

The formulation stability measurement results according to this are shown in Table 6 below.

toothpaste stickiness
(g. sec) Moisture rate
(%) density
(g/ml) Water absorption capacity (㎖/g) 1 h 3h 24h Comparative Example 1 210.3d 30.63b 1.45b 1.03d 1.07d 1.2d Comparative Example 2 293.3b 29.05bc 1.44a 1.43b 1.52b 1.63bc Example 1 531.1a 59.15a 1.32a 2.77a 3.86a 4.58a Example 2 304.0b 29.29bc 1.43a 1.45b 1.55b 1.80b Example 3 275.5c 28.39c 1.44a 1.27c 1.28c 1.42cd

According to this, the toothpaste of Example 1 containing a lot of cellulose had a high adhesiveness of 531.1, and the toothpaste of Example 3, which had a high content of stone cells, had a lower adhesiveness than the walnut shell toothpaste of Comparative Example 2, indicating higher marketability. see. The moisture content was also 59.1% in the toothpaste of Example 1 and 28 to 30% in the other toothpastes due to the high content of cellulose, and the toothpaste of Example 3 showed a relatively low moisture content of 28%. Density showed little difference between the samples, and the water absorption capacity related to the layer separation of the toothpaste and the expansion of the formulation was 1.6 for the walnut shell toothpaste of Comparative Example 1 and 1.4 for the toothpaste of Example 3, compared to 1.2 for the general toothpaste of Comparative Example 1. Among the functional toothpastes containing the abrasive composition, it showed the lowest water absorption capacity. The toothpaste of Example 3, which showed overall low adhesiveness, moisture content, and water absorption, exhibited high formulation stability similar to that of the general toothpaste of Comparative Example 1, which did not contain abrasives.

Experimental Example 4: Analysis of polishing effect of human functional toothpaste

In order to investigate the polishing effect of the toothpastes prepared according to Examples 1 to 3 and Comparative Example 1 (negative control) and Comparative Example 2 (positive control), the teeth of a 12-month-old beagle were extracted through a 3D-OP machine. Teeth and parts with similar roughness were selected and the polishing effect analysis experiment was performed.

To measure the abrasiveness of the toothpastes, first, 10 g of the sample toothpaste was applied thinly with a spatula on the satin non-woven fabric of Semi-Automatic Grinders/Polishers (Met Prep 3™ PH-4, Allied High Tech Products, Rancho Domingues, CA, USA). After marking the tooth area to be tested by 3x3cm, brushing was performed 3,000 times with a reciprocating motion of about 50 rows per minute. 3D Optical Profiler, NV-E1000; Nano System Co., Daejeon, Korea), the degree of change in roughness after fixing with rubber clay was investigated In addition, the polishing effect was determined through the arithmetic mean roughness (Ra) within the reference area. Before and after toothpaste samples were compared and measured. A photograph of the surface shape is shown in FIG. 5 .

toothpaste before polishing after polishing asperity
Ra(AB) polishing effect
(%) Ra(A) Average Ra(B) Average Comparative Example 1 4.97, 5.09, 5.08 5.05 4.78, 4.53, 4.65 4.65 0.4 7.92e Comparative Example 2 7.61, 7.51, 7.56 7.56 6.18, 5.98, 6.03 6.06 1.5 19.84c Example 1 4.01, 4.28, 4.53 4.27 3.91, 3.98, 3.83 3.91 0.36 8.43d Example 2 4.47, 4.41, 4.36 4.41 2.90, 2.98, 3.06 2.98 1.43 32.43b Example 3 4.91, 4.59, 4.77 4.76 2.68, 2.52, 2.36 2.52 2.24 47.06a

According to this, the polishing effect was 7.92% for the general toothpaste of Comparative Example 1, 19.8% for the walnut shell toothpaste of Comparative Example 2, 8.4% for the toothpaste of Example 1, 32% for the toothpaste of Example 2, and 32% for the toothpaste of Example 3. appeared at 47%. That is, the toothpaste of Example 3 having high stone cell purity had a higher abrasive effect than general toothpaste or toothpaste containing walnut shells.

Experimental Example 5: Analysis of calculus and plaque production inhibitory ability of functional toothpaste

A total of 12 experimental beagles were acclimatized in the laboratory for one week, then anesthetized to perform scaling to remove calculus, and left untreated for 4 weeks. Thereafter, 6 animals brushed their teeth twice a day for 4 weeks using commercial toothpaste (toothpaste containing 5% walnut shells), and 6 animals mixed toothpaste with the enzyme-treated abrasive powder (5%) of Preparation Example 3 and general toothpaste (Bubac C.E.T. Periaid Dental brush) was used to investigate the degree of gingival inflammation, plaque, calculus, and fecal condition, and a photograph of the scaling and brushing was shown in FIG. 6.

The remaining calculus content was qualitatively evaluated through the staining method. the irradiated area Measurements were taken at 3 sites per tooth (distal buccal, mesibuccal, buccal) of the left and right maxillary third incisors, maxillary and mandibular canines, maxillary and mandibular 3rd and 4th premolars, and maxillary and mandibular first molars. At week 0, gingivitis, plaque, and calculus evaluation progress evaluation was performed, and gingivitis, plaque and calculus removal (scale & polish) were performed under anesthesia, and fecal evaluation was performed twice a week. Gingivitis, plaque, and calculus were evaluated at weeks 2 and 4 and fecal evaluation twice a week. Brushing is performed once/day for 4 weeks, and the clinical indices evaluated at this time are the Gingival Index (GI), Plaque Index (PI), and Calculus Index (CI), and the evaluation criteria are as follows. The data obtained in all experiments were verified for significance with the Multiple Mann-Whitney test, and these values were expressed as mean ± SD values, and significance was determined as significant when the p value was less than 0.05 did

* Evaluation of gingival inflammation (GI)

The gingival inflammation status was scored using the Silness and Loe Cingival Index in the following way.

0: normal teeth

1: mild inflammation; Minor color change, mildly inflamed gingiva that is slightly swollen but does not bleed with mild irritation

2: moderate inflammation; Inflamed gingiva that is red, swollen and bleeds with minor irritation

3: severe inflammation; Gingival inflammation that has progressed to the point where marked redness, swelling, and ulceration appear and spontaneous bleeding occurs.

Gingival Inflammation Index (GI) = sum of total scores / total number of gums examined

Verdict: 0.1-1.0 (mild), 1.1-2.0 (moderate), 2.1-3.0 (severe)

* Plaque evaluation (CI)

Supragingival plaque coverage and plaque thickness were evaluated using the Turesky-Quigley-Hein Plaque index. Each tooth surface was stained with a red colorant (Erythrosin, sultan, USA) and scored in the following way. Scores are according to Table 8 below.

Plaque index (PI) = sum of (coverage * thickness) / total number of tooth surfaces examined

* Tartar Assessment (PI)

Supragingival calculus coverage and calculus thickness were scored using the Warrick and Gorrel method as follows. Scores are according to Table 9.

Gingival Inflammation Index (GI) = sum of total scores / total number of gums examined

Verdict: 0.1-1.0 (mild), 1.1-2.0 (moderate), 2.1-3.0 (severe)

* Fecal evaluation

Fecal evaluation was performed twice a week according to the Waltham Faeces Scoring system. The Waltham Faeces Scoring system evaluation criteria, which are stool evaluation criteria, are shown in FIG. 7 .

The results of measuring the gingival index (GI), plaque index (PI), and calculus index (CI) of the untreated control group without brushing are shown in FIG. 8 .

According to this, the gingival index (GI) was measured as 0.37 at the 0th week, 1.08 at the 2nd week, and 1.23 at the 4th week. Plaque index (PI) was measured as 0 at week 0, 2.96 at week 2, and 5.53 at week 4. In addition, the calculus index (CI) was measured as 0 at week 0, 1.94 at week 2, and 3.00 at week 4. In other words, compared to the 0th week, the gingival inflammation index, plaque index, and calculus index significantly increased in the 2nd and 4th weeks in the untreated control group.

On the other hand, the gingival index (GI), plaque index (PI), and calculus index (CI) were measured in the teeth of the experimental beagle treated with the toothpaste containing 5% walnut shell and the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3. The results are shown in FIG. 9 .

According to this, the gingival index (GI) at 2 weeks was measured as 1.08 in the control group, 0.65 in the group brushing with the toothpaste of Example 3, and 0.83 in the group using the commercial toothpaste containing walnut shells. In addition, the untreated control group at 4 weeks was measured at 1.23, the group brushing using the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3 was measured at 0.61, and the group using the toothpaste containing walnut shells was measured at 1.02. Compared to the untreated control group, the walnut shell-containing toothpaste decreased the gingivitis value only at the 2nd week, and the gingivitis value of the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3 was significantly reduced by 23% and 39.9% at the 2nd and 4th weeks, respectively. was found to significantly decrease.

In addition, plaque index (PI) was measured at 2.96 in the untreated control group at 2 weeks, 3.70 in the group brushing using the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3, and 3.70 in the group using the toothpaste containing walnut shells. It measured 3.36. In addition, the 4th week untreated control group was measured at 5.53, the group using the toothpaste of Example 3 was measured at 4.12, and the group using the toothpaste containing walnut shell was measured at 4.67. That is, the group using the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3 tended to have a slightly lower plaque index (PI) at 4 weeks compared to the group using the toothpaste containing walnut shells.

Calculus index (CI) was measured at 1.94 in the untreated control group at 2 weeks, 0.80 in the group brushing using the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3, and 1.00 in the group using the toothpaste containing walnut shells. has been measured In addition, the 4th week untreated control group was measured at 3.00, the calculus index of the group brushing using the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3 was 1.77, and the group using the toothpaste containing walnut shells was measured at 2.53. . That is, compared to the untreated control group, the group using the dentifrice containing the enzyme-treated abrasive powder of Preparation Example 3 showed a significant decrease in calculus index (CI) in both the 2nd and 4th weeks. As a result, at 4 weeks, when using the toothpaste containing the walnut shell-containing toothpaste and the enzyme-treated abrasive powder of Preparation Example 3, the gingivitis level was reduced by 23% or 39.9%, and the plaque index was 15.6% and 25.5%, respectively, compared to untreated. , the calculus index increased significantly by 15.7% and 41.0%, respectively, and compared to the toothpaste containing walnut shells, the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3 of the present invention was found to be particularly effective in inhibiting gingivitis and calculus.

On the other hand, a photograph showing the condition of the beagle's teeth after 4 weeks of brushing using the untreated, walnut shell-containing toothpaste and the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3 is shown in FIG. 10 . When compared with the naked eye, when the functional toothpaste of Example 3 of the present invention was applied, the condition of the teeth seemed as clean as possible.

In addition, the results of evaluating feces according to the Waltham Faeces Scoring system are shown in FIG. 11 . There was no significant difference between the groups brushed with the control group, the toothpaste containing the enzyme-treated abrasive powder of Preparation Example 3, and the toothpaste containing walnut shells.

Experimental Example 6: Sensory evaluation

10 healthy adult men and women without periodontal disease (average age for men: 33.8 years old, average age for women: 37.0 years old) were sufficiently educated on the purpose and evaluation method of the experiment, followed by Examples 1 to 3 and Comparative Examples The toothpaste prepared according to 1 and 2 was evaluated as follows. First, the subjects were taught how to brush their teeth correctly, and then toothpaste was distributed to each subject, and brushing was used in the same way as usual for 2 weeks, 3 times a day for 2 weeks, and then they were asked to evaluate the feeling of use with the control group. At this time, the sensory test scores were evaluated on a 9-point scale (9 points-very good, 7 points-excellent, 5 points-average, 3 points-less than average, 1 point-no effect) for the improvement in tooth cleanliness, respectively. The results of calculating the average value for each item for the evaluation are shown in Table 10 below.

toothpaste Exterior spreadability A feeling of freshness in the mouth Degree of bad breath removal Sustainability of coolness overall preference Comparative Example 1 7.25a 7.00ab 7.88 7.50 7.25 7.10ab Comparative Example 2 7.25a 6.75ab 7.13 6.75 7.13 7.13ab Example 1 5.88b 6.25b 6.88 6.63 6.88 5.25c Example 2 6.63ab 7.00ab 7.38 7.25 7.00 7.00ab Example 3 7.13ab 7.05ab 7.25 7.50 7.38 7.82a

According to this, it was confirmed that the toothpaste of Examples 1 to 3 exhibited higher satisfaction than the general toothpaste of Comparative Example 1 or the walnut shell-containing toothpaste of Comparative Example 2. In particular, the toothpaste of Example 3 received a score of 7.0 or higher in terms of applicability, freshness in the mouth, degree of bad breath removal, and persistence of freshness compared to commercially available toothpastes, indicating a higher preference than general products.

Although the embodiments of the present invention have been described above, those skilled in the art can add, change, delete, or add components within the scope not departing from the spirit of the present invention described in the claims. The present invention can be variously modified and changed by the like, and this will also be said to be included within the scope of the present invention.

Claims (18)

(a) heat-treating the pear pulverized solution, filtering to remove the pear juice, and drying the by-product to obtain pear pear powder;
(B) pulverizing the pear powder and then passing through a 30 mesh mesh and screening to a size that does not pass through a 60 mesh mesh to obtain a sorted pear shell powder;
(c) reacting the selected pear powder with alcohol to obtain a precipitate; and
(d) treating the precipitate with a complex enzyme containing cellulase and hemicellulase, removing the supernatant from the complex enzyme-treated product and drying the precipitate with hot air; A method for preparing an abrasive composition. According to claim 1,
In step (a), the high-pressure heat treatment is a method for producing an abrasive composition for human use, characterized in that carried out at 100 to 140 ℃. According to claim 1,
In step (c), the alcohol is a lower alcohol having 1 to 4 carbon atoms. According to claim 7,
The method for producing an abrasive composition for human use, characterized in that the alcohol is an aqueous solution of ethanol at a concentration of 40 to 50%. According to claim 7,
In step (d), the complex enzyme is added in an amount of 0.01 to 0.15 wt% of solids. According to claim 7,
In step (d), the complex enzyme is a method for producing an abrasive composition for human use, characterized in that cellulase and hemicellulase are mixed in a weight ratio of 1:0.5 to 0.5:1. According to claim 1,
In step (d), the enzyme treatment is performed at 35 to 40 ° C. for 8 to 20 hours. According to claim 1,
In step (d), the hot air drying is performed at 50 to 70 ° C. for 10 to 20 hours. An abrasive composition for human use prepared according to any one of claims 1 to 8. According to claim 9,
The human abrasive composition is characterized in that the total dietary fiber content is 75 to 85% (w / v). According to claim 9,
The abrasive composition for human use, characterized in that the insoluble dietary fiber content is 65 to 80% (w / v). According to claim 11,
An abrasive composition for human use, characterized in that the stone cell content is 85 to 95% based on the total weight of the insoluble dietary fiber. According to claim 11,
The human abrasive composition is characterized in that the water absorption capacity is 1.2 to 2㎖ / g based on the passage of 1 to 24 hours. A toothpaste composition for human use comprising the human abrasive composition prepared according to any one of claims 1 to 8. According to claim 14,
The human toothpaste composition is a human toothpaste composition, characterized in that the sugar content is 30 to 34 brix. According to claim 14,
The human abrasive composition is a human toothpaste composition, characterized in that it is included in a state of additional grinding to 500 to 800 mesh. According to claim 14,
The human toothpaste composition comprises 1 to 7 wt% of the human abrasive composition based on the total weight. According to claim 14,
The human toothpaste composition is a human toothpaste composition, characterized in that for removing or inhibiting gingivitis, plaque or calculus.

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