KR20230012554A - 결찰 결합된 pcr의 방법 - Google Patents

결찰 결합된 pcr의 방법 Download PDF

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Publication number
KR20230012554A
KR20230012554A KR1020227043950A KR20227043950A KR20230012554A KR 20230012554 A KR20230012554 A KR 20230012554A KR 1020227043950 A KR1020227043950 A KR 1020227043950A KR 20227043950 A KR20227043950 A KR 20227043950A KR 20230012554 A KR20230012554 A KR 20230012554A
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South Korea
Prior art keywords
bases
strand
oligonucleotide
temperature
indexing
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KR1020227043950A
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English (en)
Korean (ko)
Inventor
블라디미르 마카로브
조단 로세피구라
비타 라한
로버트 디. 스테드펠드
Original Assignee
인티그레이티드 디엔에이 테크놀로지스 아이엔씨.
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Publication of KR20230012554A publication Critical patent/KR20230012554A/ko
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/149—Concentration of an enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020227043950A 2020-05-15 2021-05-17 결찰 결합된 pcr의 방법 Pending KR20230012554A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063025738P 2020-05-15 2020-05-15
US63/025,738 2020-05-15
PCT/US2021/032824 WO2021232023A2 (en) 2020-05-15 2021-05-17 Methods for ligation-coupled-pcr

Publications (1)

Publication Number Publication Date
KR20230012554A true KR20230012554A (ko) 2023-01-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020227043950A Pending KR20230012554A (ko) 2020-05-15 2021-05-17 결찰 결합된 pcr의 방법

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Country Link
US (1) US20230257805A1 (https=)
EP (1) EP4150106A4 (https=)
JP (1) JP2023525880A (https=)
KR (1) KR20230012554A (https=)
CN (1) CN116249775A (https=)
AU (1) AU2021270596A1 (https=)
CA (1) CA3178211A1 (https=)
WO (1) WO2021232023A2 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11104941B2 (en) * 2018-09-28 2021-08-31 Bioo Scientific Corporation 5′ adapter comprising an internal 5′-5′ linkage
ES3036514T3 (en) * 2020-01-16 2025-09-19 Dnae Diagnostics Ltd Compositions, kits and methods for isolating target polynucleotides
CN113999893B (zh) * 2021-11-09 2022-11-01 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法
US20230323341A1 (en) * 2022-03-25 2023-10-12 Bioo Scientific Corporation Methods, compositions, and kits for inhibiting formation of adapter dimers
WO2025079564A1 (ja) * 2023-10-13 2025-04-17 アークレイ株式会社 タンパク質試料の調製方法、核酸のライゲーション方法、ｎｇｓライブラリの調製方法、及びキット

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014031954A1 (en) * 2012-08-23 2014-02-27 Tufts University Homopolymer mediated nucleic acid amplification
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
EP3114231B1 (en) 2014-03-03 2019-01-02 Swift Biosciences, Inc. Enhanced adaptor ligation
CN110012671B (zh) 2016-09-06 2024-05-28 合成Dna技术公司 Ngs文库浓度的标准化
CN110832084B (zh) * 2017-04-17 2025-06-20 吉复生物科技有限公司 用于制备核酸文库的方法、组合物和试剂盒
CN111868260B (zh) * 2017-08-07 2025-02-21 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
US11447818B2 (en) * 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019157034A1 (en) * 2018-02-07 2019-08-15 Nugen Technologies, Inc. Library preparation
CA3100739A1 (en) * 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
DK3810774T3 (da) * 2018-06-04 2023-12-11 Illumina Inc Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
SG11202100765UA (en) * 2018-08-15 2021-03-30 Illumina Inc Compositions and methods for improving library enrichment
CN111041026B (zh) * 2019-12-26 2022-03-11 北京优迅医学检验实验室有限公司 一种高通量测序用核酸接头和文库构建方法

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Publication number Publication date
US20230257805A1 (en) 2023-08-17
WO2021232023A3 (en) 2021-12-23
WO2021232023A2 (en) 2021-11-18
EP4150106A4 (en) 2024-07-10
JP2023525880A (ja) 2023-06-19
CN116249775A (zh) 2023-06-09
AU2021270596A1 (en) 2022-12-15
CA3178211A1 (en) 2021-11-18
EP4150106A2 (en) 2023-03-22

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