CN116249775A - 连接偶联pcr的方法 - Google Patents

连接偶联pcr的方法 Download PDF

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Publication number
CN116249775A
CN116249775A CN202180060917.9A CN202180060917A CN116249775A CN 116249775 A CN116249775 A CN 116249775A CN 202180060917 A CN202180060917 A CN 202180060917A CN 116249775 A CN116249775 A CN 116249775A
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bases
strand
temperature
oligonucleotide
index
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CN202180060917.9A
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English (en)
Chinese (zh)
Inventor
V·马卡洛夫
J·罗斯费格拉
B·拉罕
R·D·斯塔德菲尔德
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Integrated DNA Technologies Inc
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Integrated DNA Technologies Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/149—Concentration of an enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202180060917.9A 2020-05-15 2021-05-17 连接偶联pcr的方法 Pending CN116249775A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063025738P 2020-05-15 2020-05-15
US63/025,738 2020-05-15
PCT/US2021/032824 WO2021232023A2 (en) 2020-05-15 2021-05-17 Methods for ligation-coupled-pcr

Publications (1)

Publication Number Publication Date
CN116249775A true CN116249775A (zh) 2023-06-09

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Family Applications (1)

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CN202180060917.9A Pending CN116249775A (zh) 2020-05-15 2021-05-17 连接偶联pcr的方法

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US (1) US20230257805A1 (https=)
EP (1) EP4150106A4 (https=)
JP (1) JP2023525880A (https=)
KR (1) KR20230012554A (https=)
CN (1) CN116249775A (https=)
AU (1) AU2021270596A1 (https=)
CA (1) CA3178211A1 (https=)
WO (1) WO2021232023A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112996921A (zh) * 2018-09-28 2021-06-18 Bioo科技公司 包含内部5’-5’连接的5’衔接子

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES3036514T3 (en) * 2020-01-16 2025-09-19 Dnae Diagnostics Ltd Compositions, kits and methods for isolating target polynucleotides
CN113999893B (zh) * 2021-11-09 2022-11-01 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法
US20230323341A1 (en) * 2022-03-25 2023-10-12 Bioo Scientific Corporation Methods, compositions, and kits for inhibiting formation of adapter dimers
WO2025079564A1 (ja) * 2023-10-13 2025-04-17 アークレイ株式会社 タンパク質試料の調製方法、核酸のライゲーション方法、ｎｇｓライブラリの調製方法、及びキット

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150203887A1 (en) * 2012-08-23 2015-07-23 Tufts University Homopolymer mediated nucleic acid amplification
US20190256924A1 (en) * 2017-08-07 2019-08-22 The Johns Hopkins University Methods and materials for assessing and treating cancer
CN110832084A (zh) * 2017-04-17 2020-02-21 吉复生物科技有限公司 用于制备核酸文库的方法、组合物和试剂盒

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
EP3114231B1 (en) 2014-03-03 2019-01-02 Swift Biosciences, Inc. Enhanced adaptor ligation
CN110012671B (zh) 2016-09-06 2024-05-28 合成Dna技术公司 Ngs文库浓度的标准化
US11447818B2 (en) * 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019157034A1 (en) * 2018-02-07 2019-08-15 Nugen Technologies, Inc. Library preparation
CA3100739A1 (en) * 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
DK3810774T3 (da) * 2018-06-04 2023-12-11 Illumina Inc Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
SG11202100765UA (en) * 2018-08-15 2021-03-30 Illumina Inc Compositions and methods for improving library enrichment
CN111041026B (zh) * 2019-12-26 2022-03-11 北京优迅医学检验实验室有限公司 一种高通量测序用核酸接头和文库构建方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150203887A1 (en) * 2012-08-23 2015-07-23 Tufts University Homopolymer mediated nucleic acid amplification
CN110832084A (zh) * 2017-04-17 2020-02-21 吉复生物科技有限公司 用于制备核酸文库的方法、组合物和试剂盒
US20190256924A1 (en) * 2017-08-07 2019-08-22 The Johns Hopkins University Methods and materials for assessing and treating cancer

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112996921A (zh) * 2018-09-28 2021-06-18 Bioo科技公司 包含内部5’-5’连接的5’衔接子
CN112996921B (zh) * 2018-09-28 2024-05-03 Bioo科技公司 包含内部5’-5’连接的5’衔接子

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Publication number Publication date
KR20230012554A (ko) 2023-01-26
US20230257805A1 (en) 2023-08-17
WO2021232023A3 (en) 2021-12-23
WO2021232023A2 (en) 2021-11-18
EP4150106A4 (en) 2024-07-10
JP2023525880A (ja) 2023-06-19
AU2021270596A1 (en) 2022-12-15
CA3178211A1 (en) 2021-11-18
EP4150106A2 (en) 2023-03-22

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