CA3178211A1 - Methods for ligation-coupled-pcr - Google Patents

Methods for ligation-coupled-pcr

Info

Publication number
CA3178211A1
CA3178211A1 CA3178211A CA3178211A CA3178211A1 CA 3178211 A1 CA3178211 A1 CA 3178211A1 CA 3178211 A CA3178211 A CA 3178211A CA 3178211 A CA3178211 A CA 3178211A CA 3178211 A1 CA3178211 A1 CA 3178211A1
Authority
CA
Canada
Prior art keywords
bases
strand
oligonucleotide
temperature
double
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3178211A
Other languages
English (en)
French (fr)
Inventor
Vladimir Makarov
Jordan ROSEFIGURA
Bita LAHANN
Robert D. STEDTFELD
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integrated DNA Technologies Inc
Original Assignee
Swift Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Swift Biosciences Inc filed Critical Swift Biosciences Inc
Publication of CA3178211A1 publication Critical patent/CA3178211A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/149—Concentration of an enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3178211A 2020-05-15 2021-05-17 Methods for ligation-coupled-pcr Pending CA3178211A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063025738P 2020-05-15 2020-05-15
US63/025,738 2020-05-15
PCT/US2021/032824 WO2021232023A2 (en) 2020-05-15 2021-05-17 Methods for ligation-coupled-pcr

Publications (1)

Publication Number Publication Date
CA3178211A1 true CA3178211A1 (en) 2021-11-18

Family

ID=78525119

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3178211A Pending CA3178211A1 (en) 2020-05-15 2021-05-17 Methods for ligation-coupled-pcr

Country Status (8)

Country Link
US (1) US20230257805A1 (https=)
EP (1) EP4150106A4 (https=)
JP (1) JP2023525880A (https=)
KR (1) KR20230012554A (https=)
CN (1) CN116249775A (https=)
AU (1) AU2021270596A1 (https=)
CA (1) CA3178211A1 (https=)
WO (1) WO2021232023A2 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11104941B2 (en) * 2018-09-28 2021-08-31 Bioo Scientific Corporation 5′ adapter comprising an internal 5′-5′ linkage
ES3036514T3 (en) * 2020-01-16 2025-09-19 Dnae Diagnostics Ltd Compositions, kits and methods for isolating target polynucleotides
CN113999893B (zh) * 2021-11-09 2022-11-01 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法
US20230323341A1 (en) * 2022-03-25 2023-10-12 Bioo Scientific Corporation Methods, compositions, and kits for inhibiting formation of adapter dimers
WO2025079564A1 (ja) * 2023-10-13 2025-04-17 アークレイ株式会社 タンパク質試料の調製方法、核酸のライゲーション方法、ｎｇｓライブラリの調製方法、及びキット

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014031954A1 (en) * 2012-08-23 2014-02-27 Tufts University Homopolymer mediated nucleic acid amplification
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
EP3114231B1 (en) 2014-03-03 2019-01-02 Swift Biosciences, Inc. Enhanced adaptor ligation
CN110012671B (zh) 2016-09-06 2024-05-28 合成Dna技术公司 Ngs文库浓度的标准化
CN110832084B (zh) * 2017-04-17 2025-06-20 吉复生物科技有限公司 用于制备核酸文库的方法、组合物和试剂盒
CN111868260B (zh) * 2017-08-07 2025-02-21 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
US11447818B2 (en) * 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019157034A1 (en) * 2018-02-07 2019-08-15 Nugen Technologies, Inc. Library preparation
CA3100739A1 (en) * 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
DK3810774T3 (da) * 2018-06-04 2023-12-11 Illumina Inc Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
SG11202100765UA (en) * 2018-08-15 2021-03-30 Illumina Inc Compositions and methods for improving library enrichment
CN111041026B (zh) * 2019-12-26 2022-03-11 北京优迅医学检验实验室有限公司 一种高通量测序用核酸接头和文库构建方法

Also Published As

Publication number Publication date
KR20230012554A (ko) 2023-01-26
US20230257805A1 (en) 2023-08-17
WO2021232023A3 (en) 2021-12-23
WO2021232023A2 (en) 2021-11-18
EP4150106A4 (en) 2024-07-10
JP2023525880A (ja) 2023-06-19
CN116249775A (zh) 2023-06-09
AU2021270596A1 (en) 2022-12-15
EP4150106A2 (en) 2023-03-22

Similar Documents

Publication Publication Date Title
US20220162688A1 (en) Enhanced Adaptor Ligation
US20230257805A1 (en) Methods for ligation-coupled-pcr
US20210381042A1 (en) Methods for Adding Adapters to Nucleic Acids and Compositions for Practicing the Same
AU2018260633C1 (en) Compositions and methods for improving sample identification in indexed nucleic acid libraries
US9902994B2 (en) Method for retaining even coverage of short insert libraries
CA2948951C (en) Synthesis of double-stranded nucleic acids
US20120238738A1 (en) Oligonucleotide Adapters: Compositions and Methods of Use
KR102119431B1 (ko) 5' 보호 의존형 증폭
JP2023507529A (ja) ペアド－エンドシークエンシングのための制御された鎖置換
EP3110969A1 (en) Nucleic acid sample preparation
JP2023525880A5 (https=)
CN111542532B (zh) 酶法合成寡核苷酸的方法和体系
EP4010493B1 (en) Methods for the multiplexed isothermal amplification of nucleic acid sequences
EP4347872B1 (en) Oligo-modified nucleotide analogues for nucleic acid preparation
CN107002290B9 (zh) 样品制备方法
WO2026047131A1 (en) Templates and methods for nucleic acid synthesis
US20210262024A1 (en) Polynucleotide duplex probe molecule
JP2024528094A (ja) インデックスを付したスプリントオリゴを使用したライゲーションによる長い核酸のアセンブリー
HK40075118B (en) Methods for the multiplexed isothermal amplification of nucleic acid sequences
HK40075118A (en) Methods for the multiplexed isothermal amplification of nucleic acid sequences

Legal Events

Date Code Title Description
MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 4TH ANNIV.) - STANDARD

Year of fee payment: 4

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250428

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250428

D11 Substantive examination requested

Free format text: ST27 STATUS EVENT CODE: A-1-1-D10-D11-D117 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: REQUEST FOR EXAMINATION RECEIVED

Effective date: 20250515

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P11-P101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - VOLUNTARY AMENDMENT

Effective date: 20250515

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250515

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250516

D00 Search and/or examination requested or commenced

Free format text: ST27 STATUS EVENT CODE: A-1-1-D10-D00-D118 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: REQUEST FOR EXAMINATION REQUIREMENTS DETERMINED COMPLIANT

Effective date: 20250806

D11 Substantive examination requested

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D11-D155 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: ALL REQUIREMENTS FOR EXAMINATION DETERMINED COMPLIANT

Effective date: 20250806

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20250806

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20250806

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20250806

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD

Year of fee payment: 5

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260421

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260421