KR20220121544A - Composition for anti-microbial and anti-wrinkle comprising extract of in vitro cultured plantlet of Black currant having antioxidant activity as effective component - Google Patents

Abstract

The present invention relates to an antibacterial and skin-wrinkle reduction composition containing an extract of in-vitro cultured Ribes nigrum seedlings having antioxidant activity as an active ingredient. The extract of in-vitro cultured Ribes nigrum seedlings of the present invention has antioxidant activity and exhibits excellent antibacterial activity against Staphlyococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans and an effect of suppressing MMP-1 protein expression. Accordingly, the extract can be usefully applied as a composition for exhibiting antibacterial and skin wrinkle reduction activity.

Description

Composition for anti-microbial and anti-wrinkle comprising extract of in vitro cultured plantlet of Black currant having antioxidant activity as effective component

The present invention relates to an antibacterial and anti-wrinkle composition comprising, as an active ingredient, an extract of in-flight cultured black currant seedlings having antioxidant activity.

Photoaging refers to the phenomenon of wrinkles on the skin caused by ultraviolet rays, and the skin becomes rough, loses elasticity, becomes dry, and pigmentation such as freckles, blemishes, and blemishes occurs, and the capillaries of the skin increase and the skin becomes thinner. may be red It is known that skin wrinkles due to photoaging are more easily formed because the content of collagen, which is a major component of the skin, decreases. Collagen degrading enzymes (MMPs; matrix metalloproteinases) have a major influence on the reduction of collagen, which is involved in the decomposition of extracellular matrix and basement membrane. MMP enzyme activity is increased by ultraviolet rays, and by inhibiting it, research results have been reported that increase in skin thickness and reduce wrinkles induced by ultraviolet rays.

On the other hand, Gram-positive cocci present on the skin surface cause eczema, which is a key symptom of dermatitis, typically Staphylococcus aureus . In addition, it is known that fungi present in normal skin as well as bacteria affect the occurrence of dermatitis. When the skin barrier is damaged by scratching the skin due to dermatitis, the fungus can penetrate the skin and trigger an immune response, which can act as an exacerbation factor for dermatitis. Candida is a genus of fungi of the subphylum Imperfecta. It is a single-celled spherical or oval-shaped, present in the mouth and skin of the human body or animal. It is harmless to the human body under normal conditions. When this is weakened, it reproduces abnormally in the body, causing candidiasis. Areas with a high frequency of infection are mucous membranes such as the mouth and genitals, which cause itching or pain. Steroids, anti-inflammatory drugs, and antibacterial and antibiotics are used as drugs to treat it, but these drugs are highly toxic and have various side effects when used for a long time.

On the other hand, black currant (Black currant, Ribes nigrum ) is a deciduous broad-leaved shrub native to central northern Europe and the Himalayas, and is a plant that grows to about 1 to 2 m in height. The leaves of black currant are about 3-5 cm in length and width, are split into 3-5 parts and have irregular serrations, have no hair on the surface of the leaves, and spots are scattered on the back, and the leaves and stems are fragrant. The flowers of black currant are about 4-6mm in diameter and have a green algae red color, and hang on raceme 5-10cm long. The fruits of black currant ripen in July-August, and the diameter is about 1cm, and the surface is shiny and has a dark blue color close to black when ripe. Blackcurrants have been used as an edible and nutritious food for centuries. In Northern Europe, black currant has been consumed as an edible fruit since ancient times. It has been used as a medicinal herb since the 16th century in the Middle Ages, and herbalists have been using blackcurrant to treat bladder stones, and it is known that blackcurrant has been used as a syrup for colds and respiratory diseases.

On the other hand, Korean Patent No. 2184823 discloses 'Antioxidant composition comprising blackcurrant, lingonberry and sea buckthorn extract', and Korean Patent No. 1678301 discloses 'Thrombosis prevention containing blackcurrant fruit extract as an active ingredient' Or therapeutic pharmaceutical composition and health functional food' is disclosed, but there is no description about the 'antibacterial and skin wrinkle-improving composition containing the extract of blackcurrant in-flight cultured seedlings having antioxidant activity as an active ingredient' of the present invention.

The present invention was derived from the above needs, and the present inventors confirmed the excellent antioxidant effect of the in-flight cultured blackcurrant extract, Staphlyococcus aureus , Escherichia coli , Pseudomonas aeruginosa , and Candida albicans ( Candida albicans ) By confirming the antibacterial activity and the inhibitory effect on the expression of MMP-1 protein, the present invention was completed.

In order to solve the above problems, the present invention provides a cosmetic composition for antibacterial and anti-wrinkle improvement containing blackcurrant in-flight seedling extract as an active ingredient.

In addition, the present invention provides a health functional food composition for antibacterial and anti-wrinkle improvement containing blackcurrant in-flight seedling extract as an active ingredient.

Blackcurrant in-flight seedling extract of the present invention has antioxidant activity, Staphlyococcus aureus ), Escherichia coli , Pseudomonas aeruginosa ) and Candida albicans ) and MMP-1 Since the protein expression inhibitory effect is excellent, it is expected to be very useful as a composition for antibacterial and skin wrinkle improvement.

1 is a result confirming the toxicity of the extract of blackcurrant in-flight cultured seedlings in human fibroblast cell line CCD-986SK cells.
Figure 2 is the result of confirming the DPPH free radical scavenging ability of the blackcurrant in-flight seedling extract. Vit. C is vitamin C used as a positive control. Different letters a~e in the drawing mean that there is a significant difference from each other, and p<0.05.
3 is a result of confirming the ABTS free radical scavenging ability of the in-flight cultured seedling extract of blackcurrant. Vit. C is vitamin C used as a positive control. Different letters a to f in the drawing mean that there is a significant difference from each other, and p<0.05.
4 is a result of confirming the expression level of MMP-1 according to the black currant in-flight seedling extract treatment. The control was a control group that was not treated with anything, and adenosine was used as a positive control as adenosine, known as a wrinkle-improving ingredient. Different letters a to d in the drawing mean that there is a significant difference from each other, and p<0.05.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for antibacterial and anti-wrinkle improvement containing blackcurrant in-flight seedling extract as an active ingredient.

The blackcurrant in-flight cultured seedlings are preferably those received from Chonnam National University of Science and Technology, a joint research institute (Korea Patent No. 1836619), but are not limited thereto.

The extraction solvent of the blackcurrant in-flight seedling extract of the present invention is preferably a C ₁ to C ₄ lower alcohol, water, or a mixture thereof, and more preferably ethanol, but is not limited thereto.

All conventional methods known in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction, may be used for the extraction method of the blackcurrant in-flight seedling extract of the present invention. The extraction solvent is preferably extracted by adding 1 to 20 times the weight of the in-flight cultured blackcurrant seedlings, and more preferably adding 5 to 15 times, but is not limited thereto. The extraction temperature is preferably 20 to 60 °C, more preferably 30 to 50 °C, but is not limited thereto. In addition, the extraction time is preferably 4 to 8 hours, more preferably 5 to 7 hours, but is not limited thereto.

In addition, the blackcurrant in-flight seedling extract is an extract obtained by extraction treatment; dilutions or concentrates of extracts; a dried product obtained by drying the extract; modifiers; Or it may include any one of the purified product.

The blackcurrant in-flight seedling extract of the present invention has excellent antioxidant activity, and has antibacterial activity against Staphlyococcus aureus , Escherichia coli , Pseudomonas aeruginosa , and Candida albicans , The effect of inhibiting the expression of MMP-1 protein is excellent.

The cosmetic composition for antibacterial and skin wrinkle improvement of the present invention is a cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent , milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, nourishing essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser It may have any one selected formulation, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily determined by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

In addition, the present invention provides a health functional food composition for antibacterial and anti-wrinkle improvement containing blackcurrant in-flight seedling extract as an active ingredient.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient may be used appropriately depending on the purpose of its use (prevention or improvement). In general, in the production of food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of the health functional food. Examples of foods to which the health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, vitamin complexes, etc., and includes all health foods in the ordinary sense.

In addition, the health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention may include ingredients that are commonly added. Examples include proteins, carbohydrates, fats, nutrients and seasonings. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrin, cyclodextrin, etc.) or sugar alcohols (eg, , xylitol, sorbitol, erythritol, etc.). As the flavoring agent, natural flavoring agents (eg, taumatine, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonation agent and the like used in beverages. The ratio of these added ingredients is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

Hereinafter, the present invention will be described in more detail using examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited thereto.

Preparation Example 1. Preparation of blackcurrant in-flight seedling extract

(1) Preparation of in-flight cultured blackcurrant seedlings

Young eyes of branches that did not undergo lignification were collected from blackcurrant seedlings, disinfected using a surfactant, alcohol, and sodium hypochlorite, and then washed with sterile water. Thereafter, the sterilized young eyes in a shoot-forming medium containing 20-50 g of sucrose and 2-5 g of phyta gel per 1 liter of MS (Murashigae and Skoog) basic medium, at 22-27 ° C. at 2000-3000 lux for 15-17 hours Inducing shoot formation by culturing for 14-21 days while irradiating light. After the shoots are formed, the nodular shoots cut into nodes are transferred to the seedlings with 1~5mg of BAP per 1 liter of MS basic medium and irradiated with light at 22~27℃ at 2000~3000lux for 15~17 hours. The growth of node seedlings was induced by culturing for ~35 days. The proliferated node seedlings were cultured for 14 to 21 days while irradiating light for 15 to 17 hours at 2000 to 3000 lux at 22 to 27 ° C in a node seedling growth medium containing 0.1 to 3 mg of BAP per 1 liter of MS basal medium, followed by culturing for 14 to 21 days in height and diameter. were grown, and in a rooting medium containing 1 to 3 mg of one selected from IBA (Indole butyric acid), NAA (1-Naphtalene acetic acid) and IAA (Indole acetic acid) per 1 liter of MS basal medium, 2000~ at 22~27℃ Rooted by culturing for 13 to 15 days while irradiating light at 3000 lux for 15 to 17 hours. Then, the rooted seedlings were acclimatized to the soil to prepare blackcurrant in-flight cultured seedlings.

(2) Preparation of black currant in-flight seedling extract

70% (v/v) ethanol was added 10 times to the blackcurrant in-flight cultured seedlings prepared by the above method, extracted at 40° C. for 6 hours, filtered with filter paper, concentrated and freeze-dried, and used for subsequent experiments.

Example 1. Cytotoxicity analysis of blackcurrant in-flight seedling extract

The human fibroblast line CCD-986SK (ATCC, CRL-1947 ^TM ) was cultured in Dulbecco Modified Eagle Medium (DMEM) medium containing 10% Heat Inactivated Fetal Bovine Serum (HIFBS) and 1% penicillin-streptomycin. cultured in CCD-986SK cells were seeded in a 96-well plate at 1×10 ⁴ cells/well and cultured at 37° C., 5% CO ₂ for 24 hours in a cell incubator for stabilization, followed by 5, 10, 25, 50 and 100 μg/ml 100 μl of blackcurrant in-flight seedling extract was added to each well and cultured for 24 hours. After removing the culture medium, 5 mg/ml of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added to each well and incubated for 2 hours, then the supernatant was removed and DMSO (dimethyl sulfoxide) was dispensed by 100 μl to dissolve all crystals generated in each well, absorbance was measured at 540 nm using a microplate reader (Multiskan Go, Thermo Scientific, USA), and cell viability was analyzed using the following formula. did.

Cell viability (%) = [absorbance of sample treatment group / absorbance of control group] X 100

As a result, it was confirmed that 5, 10, 25, 50 and 100 μg/ml of in vitro cultured blackcurrant extracts did not show cytotoxicity in the human fibroblast cell line CCD-986SK ( FIG. 1 ).

Example 2. Analysis of antioxidant activity of blackcurrant in-flight seedling extract

(1) Measurement of flavonoid content

Dissolve 1 mg of freeze-dried blackcurrant in-flight seedling extract in 1 ml of methanol, add 10 ml of diethylene glycol, mix well, add 1 ml of 1N NaOH, and react at 37° C. for 1 hour, then 420 nm absorbance was measured. At this time, quercetin was used as a standard material, and the total flavonoid content was calculated from the standard curve obtained after analysis in the same manner as the sample.

As a result, it was confirmed that the total flavonoid content in the blackcurrant in-flight seedling extract was 46.50±3.91 mg/g.

(2) Measurement of polyphenol content

It was prepared by dissolving 1 mg of freeze-dried blackcurrant in-flight seedling extract in 1 ml of methanol and diluting 10 times. 2 ml of a 2-fold diluted phenol reagent (Folin-Ciocalteu's phenol reagent) was added to 2 ml of the diluent, mixed, and left for 3 minutes, then 2 ml of a 10% Na ₂ CO ₃ solution was added and reacted for 1 hour, Absorbance was measured at 750 nm. At this time, gallic acid was used as the standard material, and the total polyphenol content was calculated from the standard curve obtained after analysis in the same manner as the sample.

As a result, it was confirmed that the total polyphenol content in the blackcurrant in-flight seedling extract was 41.35±0.07 mg/g.

(3) Measurement of DPPH free radical scavenging ability

Blackcurrant in-flight seedling extracts were prepared at concentrations of 25, 50, 100, 200 and 500 μg/ml, and DPPH (1,1-diphenyl-2-picrylhydrazyl) was dissolved in ethanol to prepare a 0.1 mM DPPH solution. Blackcurrant incubated seedling extract of each concentration and 0.1 mM DPPH solution were mixed 1:1, treated in a 96-well plate, reacted at 37°C for 30 minutes, absorbance was measured at 517 nm, and DPPH free radicals were calculated using the following formula. The scavenging ability was calculated.

DPPH free radical scavenging ability (%) = ｛1-(A-B/C)×100

A: Absorbance after reacting the sample with DPPH

B: Absorbance after reacting the sample with ethanol

C: Absorbance (blank) after reacting ethanol and DPPH

As a result, it was confirmed that the DPPH free radical scavenging activity of the blackcurrant in-flight seedling extract increased in a concentration-dependent manner (FIG. 2).

(4) ABTS free radical scavenging activity measurement

Blackcurrant in-flight seedling extracts were prepared at concentrations of 25, 50, 100, 200 and 500㎍/㎖, 7mM ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) and 2.4 An ABTS solution was prepared by mixing mM potassium persulfate in a 1:1 ratio. Blackcurrant incubated seedling extract of each concentration and the ABTS solution were mixed 1:1, treated in a 96-well plate, reacted at room temperature for 30 minutes, absorbance was measured at 415 nm, and ABTS free radical scavenging ability was evaluated using the following formula calculated.

ABTS radical scavenging ability (%) = [(A-B)/A]×100

A: control absorbance

B: absorbance of sample group

As a result, it was confirmed that the ABTS free radical scavenging ability of the blackcurrant in-flight seedling extract increased in a concentration-dependent manner (FIG. 3).

Example 3. Analysis of antibacterial activity of blackcurrant in-flight seedling extract

(1) Measurement of antibacterial activity by disc-diffusion method

Staphlyococcus aureus ( ATCC 6538), E. coli ( Escherichia coli , ATCC 9839) or Pseudomonas aeruginosa ( ATCC 9027) was inoculated in TSB (tryptic soy broth) medium and pre-incubated at 30-35° C. for 18 hours. After culturing, it was prepared by plating on a solid medium.

Blackcurrant in-flight seedling extract was dripped at 3 mg/disk and 6 mg/disc on a sterilized paper disk, and Staphylococcus aureus ( S. aureus ), E. coli ( E. coli ) or Pseudomonas aeruginosa ( P. aeruginosa ) was smeared above. It was placed on a solid medium and cultured for 24 hours in an incubator at 30-35° C., and then the diameter of the growth-stopping ring (clear zone) was measured to confirm the antibacterial activity. 1,2-hexanediol (1,2-Hexanediol) and caprylyl glycol, known as preservatives, were prepared at 10 mg/disc and used as a control.

As a result, it was found that the blackcurrant in-flight seedling extract had antibacterial activity against Staphylococcus aureus ( S. aureus ), E. coli or P. aeruginosa ( Tables 1, 2 and 3). .

For S. aureus of blackcurrant in-flight seedling extract Determination of antibacterial activity sample Diameter of growth-stopping ring (mm) S. aureus Blackcurrant in-flight seedling extract 3mg/disc 0.5 Blackcurrant in-flight seedling extract 6mg/disc 0.5 1,2-Hexanediol 0.1 Caprylyl glycol 3.0

E. coli of blackcurrant in-flight seedling extract Determination of antibacterial activity sample Diameter of growth-stopping ring (mm) E. coli Blackcurrant in-flight seedling extract 3mg/disc - Blackcurrant in-flight seedling extract 6mg/disc 0.2 1,2-Hexanediol 1.5 Caprylyl glycol 5.0

For P. aeruginosa of blackcurrant in-flight seedling extract Determination of antibacterial activity sample Diameter of growth-stopping ring (mm) P. aeruginosa Blackcurrant in-flight seedling extract 3mg/disc 1.0 Blackcurrant in-flight seedling extract 6mg/disc 1.5 1,2-Hexanediol 0.5 Caprylyl glycol 1.0

(2) Analysis of antibacterial activity by measuring the minimum inhibitory concentration (MIC)

Staphlyococcus aureus ( ATCC 6538), Escherichia coli ( ATCC 9839) or Pseudomonas aeruginosa ( ATCC 9027) was inoculated in TSB (tryptic soy broth) medium, Candida albicans ( Candida albicans , ATCC 10231) was inoculated in PDB (potato dextrose broth) medium and pre-cultured for 18 hours in an incubator at 30-35 ° C. Staphylococcus aureus ( S. aureus ), Escherichia coli ( E. coli ) or Pseudomonas aeruginosa ( P. aeruginosa ) was diluted in sterile physiological saline, and Candida albicans ( C. albicans ) was sterilized by adding Tween 80 at a rate of 0.05%. It was diluted in saline to prepare a bacterial solution of 10 ⁶ /ml, respectively.

The in-flight cultured seedling extract of blackcurrant was diluted two-fold and prepared in 10 steps, each inoculated dropwise with the prepared bacterial solution, cultured at 25~32℃ for 1~3 days, and then the growth of the strain was observed with the naked eye. The minimum inhibitory concentration (MIC) for each bacteria was determined.

As a result, the minimum inhibitory concentration against Staphylococcus aureus ( S. aureus ) and Pseudomonas aeruginosa ( P. aeruginosa ) of the blackcurrant in-flight seedling extract was 0.5%, and Escherichia coli ( E. coli ) and Candida albicans ( C. albicans ) It was confirmed that the minimum inhibitory concentration was 2.0% (Table 4).

Minimum inhibitory concentration (MIC) measurement of blackcurrant in-flight seedling extract strain Minimum inhibitory concentration (%) S. aureus 0.5 E. coli 2.0 P. aeruginosa 0.5 C. albicans 2.0

Example 4. Analysis of skin wrinkle improvement activity of blackcurrant in-flight seedling extract

Matrix metalloproteinase-1 (MMP-1) is an enzyme that degrades collagen protein expressed in the skin. When the expression level of MMP-1 increases, collagen in the dermal layer of the skin is decomposed and wrinkles are generated. Therefore, after irradiating the human fibroblast line CCD-986SK with UV light, each sample was treated and the expression level of MMP-1 was measured to analyze the skin wrinkle improvement activity.

CCD-986SK cells were seeded on a 60mm plate at 4×10 ⁵ cells/plate and cultured at 37°C, 5% CO ₂ in an incubator for 24 hours, then the medium was removed, PBS was added, and UVB (UVB, 312 nm) 10mJ was applied. investigated. Then, 25, 50 and 100㎍ / ㎖ of blackcurrant in-flight seedling extracts were treated and cultured for 24 hours, the cell culture was recovered and the protein was quantified using a BCA protein content measurement kit (Thermo scientific PIERCE, USA). After that, the expression level of MMP-1 was measured using the MMP-1 ELISA kit (sigma).

As a result, it was confirmed that the MMP-1 expression level of the blackcurrant in-flight seedling extract treatment group was decreased compared to the control group that was not treated with anything. In particular, it was confirmed that 50 and 100 μg/ml of in-flight cultured black currant seedling extract had a superior effect on inhibiting MMP-1 expression than adenosine, which is known as a wrinkle-improving component ( FIG. 4 ).

Through the above results, it was found that the extract of blackcurrant in-flight seedlings had skin wrinkle improvement activity by reducing the expression of MMP-1.

[Statistics processing]

All data of the present invention were expressed as mean ± standard error, and statistical processing of the data was analyzed using SPSS (Statistical Package for Social Science, Chicago, IL, USA). One-way ANOVA was performed between groups, and the significance of each section was determined by Duncan test, and the significance of the mean values was verified at a confidence level of p<0.05.

Claims (6)

A cosmetic composition for antibacterial and anti-wrinkle improvement containing blackcurrant in-flight seedling extract as an active ingredient. The cosmetic composition for antibacterial and skin wrinkle improvement according to claim 1, wherein the extraction solvent of the blackcurrant in-flight seedling extract is C ₁ to C ₄ lower alcohol, water, or a mixture thereof. [Claim 2] The cosmetic composition for antibacterial and skin wrinkle improvement according to claim 1, wherein the cosmetic composition has antioxidant activity. According to claim 1, wherein the antibacterial is Staphlyococcus aureus , Escherichia coli , Pseudomonas aeruginosa , and Candida albicans ( Candida albicans ) Antibacterial and skin wrinkle improvement, characterized in that the antibacterial activity. cosmetic composition for The cosmetic composition for antibacterial and skin wrinkle improvement according to claim 1, wherein the cosmetic composition reduces the expression level of MMP-1 protein. A health functional food composition for antibacterial and anti-wrinkle improvement containing blackcurrant in-flight seedling extract as an active ingredient.

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