KR20220114630A - 복수의 생물학적 액체 샘플에서 관심 분석물을 검출 및/또는 정량하는 방법 - Google Patents

복수의 생물학적 액체 샘플에서 관심 분석물을 검출 및/또는 정량하는 방법 Download PDF

Info

Publication number
KR20220114630A
KR20220114630A KR1020227024601A KR20227024601A KR20220114630A KR 20220114630 A KR20220114630 A KR 20220114630A KR 1020227024601 A KR1020227024601 A KR 1020227024601A KR 20227024601 A KR20227024601 A KR 20227024601A KR 20220114630 A KR20220114630 A KR 20220114630A
Authority
KR
South Korea
Prior art keywords
microparticles
analyte
subset
sample
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020227024601A
Other languages
English (en)
Korean (ko)
Inventor
오이겐 에어만트라우트
카트린 슈타인메트쩨어
슈테판 후볼트
토마스 엘링어
올리버 레무트
Original Assignee
블링크 아게
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 블링크 아게 filed Critical 블링크 아게
Publication of KR20220114630A publication Critical patent/KR20220114630A/ko
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
KR1020227024601A 2019-12-16 2020-12-15 복수의 생물학적 액체 샘플에서 관심 분석물을 검출 및/또는 정량하는 방법 Pending KR20220114630A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19216592.6 2019-12-16
EP19216592.6A EP3839508A1 (en) 2019-12-16 2019-12-16 A method of detecting and/or quantitating an analyte of interest in a plurality of biological liquid samples
PCT/EP2020/086194 WO2021122579A1 (en) 2019-12-16 2020-12-15 A method of detecting and/or quantitating an analyte of interest in a plurality of biological liquid samples

Publications (1)

Publication Number Publication Date
KR20220114630A true KR20220114630A (ko) 2022-08-17

Family

ID=68917648

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020227024601A Pending KR20220114630A (ko) 2019-12-16 2020-12-15 복수의 생물학적 액체 샘플에서 관심 분석물을 검출 및/또는 정량하는 방법

Country Status (13)

Country Link
US (1) US20230063987A1 (https=)
EP (2) EP3839508A1 (https=)
JP (1) JP2023506261A (https=)
KR (1) KR20220114630A (https=)
CN (1) CN115151819A (https=)
AU (1) AU2020403853A1 (https=)
BR (1) BR112022011676A2 (https=)
CA (1) CA3161939A1 (https=)
DK (1) DK4078181T3 (https=)
ES (1) ES2980821T3 (https=)
FI (1) FI4078181T3 (https=)
IL (1) IL293851B2 (https=)
WO (1) WO2021122579A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2022205377A1 (en) 2021-01-08 2023-07-20 Cellanome, Inc. Devices and methods for analyzing biological samples
WO2022150659A1 (en) * 2021-01-08 2022-07-14 Cellanome, Inc. Devices and methods for analyzing biological samples
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
EP4177352A1 (en) 2021-11-05 2023-05-10 Blink AG A microsphere comprising a first material and a second material
EP4252907A1 (en) 2022-03-30 2023-10-04 Blink AG A method for arranging microspheres in a non-aqueous liquid phase
EP4438737A1 (en) * 2023-03-31 2024-10-02 Blink AG A method of quantifying a concentration of an analyte in a sample
EP4732949A1 (en) * 2023-06-22 2026-04-29 Tantti Laboratory Inc. Micro-droplet production device and method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1470873A (zh) * 2002-07-22 2004-01-28 � 赵 Dna固相扩增与流式检测分析技术与试剂盒
EP3136103B1 (en) * 2007-10-05 2018-08-29 Affymetrix, Inc. Highly multiplexed assays
CN114471756B (zh) * 2013-11-18 2024-04-16 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
EP3343223A1 (en) * 2016-12-30 2018-07-04 Blink AG A prefabricated microparticle for performing a digital detection of an analyte

Also Published As

Publication number Publication date
FI4078181T3 (fi) 2024-05-07
EP4078181A1 (en) 2022-10-26
WO2021122579A1 (en) 2021-06-24
BR112022011676A2 (pt) 2022-10-11
ES2980821T3 (es) 2024-10-03
CN115151819A (zh) 2022-10-04
EP4078181B1 (en) 2024-03-20
US20230063987A1 (en) 2023-03-02
IL293851A (en) 2022-08-01
JP2023506261A (ja) 2023-02-15
IL293851B2 (en) 2024-03-01
CA3161939A1 (en) 2022-06-14
EP3839508A1 (en) 2021-06-23
DK4078181T3 (da) 2024-05-13
IL293851B1 (en) 2023-11-01
AU2020403853A1 (en) 2022-07-07

Similar Documents

Publication Publication Date Title
KR20220114630A (ko) 복수의 생물학적 액체 샘플에서 관심 분석물을 검출 및/또는 정량하는 방법
US20240093291A1 (en) Spatial genomics with single cell resolution
CA3012680C (en) Isotachophoresis for purification of nucleic acids
US20240033739A1 (en) Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
US11814619B2 (en) Compositions, methods, and systems for single cell barcoding and sequencing
JP7843040B2 (ja) 製作済み微粒子およびその前駆体のライブラリー
JP2023506261A5 (https=)
WO2022256612A1 (en) Compositions, methods, and systems for single cell barcoding and sequencing
US20240254549A1 (en) Partitioning cells for high throughput single-cell sequencing
US20230142197A1 (en) Cation exchange for sperm-associated dna purification
JP2025542198A (ja) 分析物を単離するための方法、システム、および組成物
US20250051834A1 (en) Single-Cell Epigenomic Profiling Using Droplet Fluidics and Hydrogels
WO2026006284A1 (en) Passive reactions consumables
WO2025136701A1 (en) Passive encapsulated workflows
CN120981581A (zh) 一种定量样品中分析物的浓度的方法

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

D13-X000 Search requested

St.27 status event code: A-1-2-D10-D13-srh-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11 Administrative time limit extension requested

Free format text: ST27 STATUS EVENT CODE: U-3-3-T10-T11-OTH-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

D22 Grant of ip right intended

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D22-EXM-PE0701 (AS PROVIDED BY THE NATIONAL OFFICE)

PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701