KR20220109542A - Small particles water by the ultravacuum microwave process - Google Patents

Abstract

The present invention relates to micro-clustered water in which the frequency of water is 50 Hz or less. The micro-clustered water has various physiological activities even though the cluster of water is small, thereby being used in various fields such as health functional food, cosmetics, and medicine.

Description

Small particle count by ultra-vacuum microwave method {SMALL PARTICLES WATER BY THE ULTRAVACUUM MICROWAVE PROCESS}

The present invention relates to the number of small particles by the ultra-vacuum microwave method and the utilization of the number of small particles.

Water occupies 70% of the earth's surface and more than 70% of the human body. Drinking water reaches the blood within 1 minute, and can reach every part of the body in 30 minutes. There is no ingredient that directly affects our body as much as water. So, recently, there is a strong perception that water is good water that goes beyond the passive dimension of pure water from which contaminants and toxic substances have been removed, actively maintains the health of the body and enables healing from diseases.

A water molecule is bonded with two hydrogen atoms and one oxygen atom, but it does not exist as a single molecule, and several to tens of molecules exist as a mass through hydrogen bonds. This mass of water molecules is called a water cluster. Usually, a water cluster is made up of 15-20 water molecules, so it is difficult to pass through the cell membrane, so absorption is slow in our body. Water of microclusters, in which 5-6 water molecules are agglomerated, that is, water of small particles (also called the number of small particles) is quickly absorbed by our body.

The water cluster can be measured as a frequency (Hz) of a signal detected using NMR (nuclear magnetic resonance). That is, it can be said that the lower the frequency (Hz), the closer to the micro cluster (the number of small particles). It is known that cells in our body absorb 60Hz small particle water best. If you drink ionized water after sweating or a hangover, the reason the body absorbs it well is because the cluster of ionized water is low. If you eat watermelon when it's hot in summer, it absorbs well and feels cool because the water cluster in the watermelon is about 75Hz. Generally, the water cluster in vegetables and fruits is 70-80 Hz. Therefore, many researchers have made a lot of effort to make the water cluster number less than 60 Hz.

Republic of Korea Patent Registration No. 10-1578687

An object of the present invention is to provide a number of small particles having various physicochemical or physiological properties while the number of clusters is 50 Hz or less using the ultra-vacuum microwave method, and a food, cosmetic, or pharmaceutical composition using the same.

In one aspect, the present invention provides the number of small particles as the number of small particles, wherein the number of particles of water is 50 Hz or less.

In an exemplary embodiment, the number of small particles may be extracted by ultra-vacuum microwave extraction method.

In an exemplary embodiment, the number of small particles may be extracted from vegetables or fruits.

In one aspect, the present invention provides a composition for activating AMP-dependent protein kinase (AMPK) comprising the number of small particles.

In another aspect, the present invention provides an antioxidant composition comprising the small number of particles.

In another aspect, the present invention provides a composition for inhibiting rust formation comprising the small number of particles.

In another aspect, the present invention provides a composition for plant growth comprising the number of small particles.

In one aspect, the present invention provides a food, cosmetic or pharmaceutical composition comprising the small number of particles.

In one aspect, the present invention can provide a small particle number with a low particle number.

In one aspect, the number of small particles according to an embodiment of the present invention can significantly activate AMPK, which functions as a sensor to control energy metabolism by detecting that the body needs and does not need energy in the body. The efficacy of AMPK as an agonist is very significant as it can be used as a basis for the development of all metabolic-related products such as diabetes, hypertension, obesity, and exercise.

In one aspect, the number of small particles according to an embodiment of the present invention is excellent in antioxidant activity. This antioxidant action is very effective in removing free radicals that are the cause of various diseases and the main cause of aging.

In one aspect, the number of small particles according to an embodiment of the present invention is excellent in the effect of inhibiting rust.

In one aspect, the number of small particles according to an embodiment of the present invention can promote the growth of vegetables and fruits such as sprouted barley, radish, red pepper, spinach, Chinese cabbage, green onion, tomato, melon, etc. can

1 is a result of measuring the frequency (Hz) of the number of small particles according to an embodiment of the present invention.
2 is a photograph showing an apparatus for producing small particles by an ultra-vacuum microwave method in one aspect of the present invention.
3 is a graph showing the antioxidant capacity of the number of small particles according to an embodiment of the present invention.
4 is a Western blotting result showing the AMPK activity of the number of small particles according to an embodiment of the present invention.
5 is a photograph showing the rust formation inhibitory ability of the number of small particles according to an embodiment of the present invention.
6 is a photograph showing barley sprouts grown using the number of small particles according to an embodiment of the present invention.
7 is a photograph showing radish grown using the number of small particles according to an embodiment of the present invention.

Hereinafter, prior to being described in detail through preferred embodiments of the present invention, terms or words used in the present specification and claims should not be construed as being limited to conventional or dictionary meanings, and meanings consistent with the technical spirit of the present invention. and should be interpreted as a concept.

Throughout this specification, when a part "includes" a component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

As used throughout this specification, the terms "about," "substantially," and the like are used in a sense at or close to the numerical value when the manufacturing and material tolerances inherent in the stated meaning are presented, and are used in the understanding of the present invention. It is used to prevent an unconscionable infringer from using the disclosure in which exact or absolute figures are mentioned to help the user.

One aspect The present invention provides the number of small particles extracted by the ultra-vacuum microwave extraction method. This ultra-vacuum microwave extraction method is described in detail in Korean Patent Application No. 10-2015-0035001.

A water molecule has a structure of H ₂ O that is bonded with two hydrogen atoms and one oxygen atom, but does not exist as a single molecule, but about 3 to 4 to dozens of molecules exist as a mass through hydrogen bonds. The mass is called a cluster of water. Such a water cluster may be measured as a frequency (Hz) of a signal detected using a nuclear magnetic resonance (NMR). In the present specification, the number of small particles refers to water having a small water cluster, that is, water having a small frequency (Hz) measured using a nuclear magnetic resonance device.

In one aspect, the number of small particles according to an embodiment of the present invention may be 50 Hz or less of the number of particles of water. Specifically, the number of small particles is the number of particles of water 50 Hz or less, 49 Hz or less, 48 Hz or less, 47 Hz or less, 46 Hz or less, 45 Hz or less, 44 Hz or less, 43 Hz or less, 42 Hz or less, 41 Hz or less, or 40 Hz or less, 1 Hz or more, 2 Hz or more, 3 Hz or more, 4 Hz or more, 5 Hz or more, 6 Hz or more, 7 Hz or more, 8 Hz or more, 9 Hz or more, 10 Hz or more, 11 Hz or more, 12 Hz or more, 13 Hz or more, 14 Hz or more, 15 Hz or more, 16 Hz or more, 17 Hz or more, 18 Hz or more, 19 Hz or more, 20 Hz or more.

In one aspect, the number of small particles according to an embodiment of the present invention may be extracted from vegetables or fruits, but is not necessarily limited thereto. For example, the number of small particles may be extracted from mistletoe, barley sprout, aronia, and the like. In addition, the number of small particles may be extracted from food containing water.

In one aspect, the number of small particles according to an embodiment of the present invention may activate AMP-activated protein kinase (AMPK). Therefore, the present invention can provide a composition for activating AMP-activated protein kinase (AMPK) comprising the small number of particles as an active ingredient. AMPK is closely related to most of the metabolism occurring in the body as well as the physiological activities of many biological organs, and is also an important regulator of metabolic activity. In animals, including humans, AMPK is involved in maintaining blood sugar homeostasis and appetite, and is closely related to physiological changes caused by exercise.

In one aspect, the number of small particles according to an embodiment of the present invention may have an antioxidant action. Therefore, the present invention can provide an antioxidant composition comprising the number of small particles as an active ingredient.

As used herein, "rust" refers to oxides, hydroxides, carbonates, etc. generated on the surface of a metal by the action of oxygen, carbon dioxide gas, moisture, etc. in the atmosphere. For example, the main components of rust generated in steel are ferrous hydroxide, ferric hydroxide, iron carbonate, etc., and since they are absorbent, once eroded, rust generation proceeds rapidly. Also, rusting is promoted by ionization.

In one aspect, the number of small particles according to an embodiment of the present invention can suppress rust formation. Therefore, the present invention can provide a composition for inhibiting rust formation comprising the number of small particles as an active ingredient. The composition for inhibiting rust formation may include, for example, water-based paint dilution water, automatic yarn washing water, water for cement mixing, and the like, but is not limited thereto.

In one aspect, the number of small particles according to an embodiment of the present invention can promote plant growth. Accordingly, the present invention can provide a composition for plant growth comprising the number of small particles as an active ingredient. The plant may include vegetables such as sprouted barley, radish, red pepper, spinach, Chinese cabbage, green onion, tomato, melon, and the like, fruits and the like.

In addition, the composition for plant growth of the present invention can increase the productivity of plants, and can be used as a fertilizer or the like. In particular, when the number of small particles of the same type is used, for example, in the case of bean sprouts, the number of small particles extracted from bean sprouts, in the case of tomatoes, the number of small particles extracted from tomatoes, and in the case of radish, the number of small particles extracted from radish is used. can be maximized.

In one aspect, the present invention may provide a food composition comprising the number of small particles. The formulation of the food composition is not particularly limited, but, for example, may be formulated in tablets, granules, pills, powders, liquids such as drinks, caramel, gels, bars, tea bags, and the like. In addition to the active ingredient, the food composition of each formulation can be appropriately selected and formulated by those skilled in the art without difficulty depending on the formulation or purpose of use in addition to the active ingredient, and when applied simultaneously with other raw materials, a synergistic effect may occur.

The food composition according to an embodiment of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, colorants and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the food compositions according to an embodiment may include natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not so important, but is generally included in the range of 0 to about 50 parts by weight per 100 parts by weight of the composition according to an embodiment.

In one aspect, the present invention may provide a cosmetic composition comprising the small number of particles. The cosmetic composition is, for example, softening lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, eye cream, eye essence, essence, cleansing cream, cleansing lotion, cleansing foam, cleansing water, pack, powder, body lotion , body cream, body essence, body cleaner, hair dye, shampoo, conditioner, hair dressing, hair conditioner, ointment, gel, cream, patch, spray, powder, and skin adhesion type, but are not limited thereto.

In addition, in each formulation, other components other than the above essential components can be appropriately selected and formulated by those skilled in the art without difficulty depending on the type or purpose of use of other external preparations.

The cosmetic composition may be provided in any formulation suitable for topical application. For example, it may be provided in the form of a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a microneedle, a foam, or an aerosol composition. have. Compositions of such formulations can be prepared according to conventional methods in the art.

The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the compounds of the present specification. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract. The cosmetic composition according to an embodiment of the present invention may include other ingredients that can preferably give a synergistic effect to the main effect within a range that does not impair the main effect. In addition, the cosmetic composition according to an embodiment of the present invention may further include a moisturizer, an emollient, a surfactant, a UV absorber, a preservative, a disinfectant, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cooling agent or a limiting agent. have. The blending amount of the component can be easily selected by those skilled in the art within the range that does not impair the purpose and effect of the present specification, and the blending amount may be 0.001 to 10 wt%, specifically 0.01 to 3 wt%, based on the total weight of the composition have.

In one aspect, the present invention may provide a pharmaceutical composition comprising the number of small particles. The pharmaceutical composition may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously or the like. Formulations for oral administration may be tablets, pills, soft and hard capsules, granules, powders, fine granules, liquids, emulsions, or pellets, but are limited thereto it is not Formulations for parenteral administration may be solutions, suspensions, emulsions, gels, injections, drops, suppositories, patches, or sprays, but is not limited thereto. The formulations can be easily prepared according to conventional methods in the art, and surfactants, excipients, wetting agents, emulsification accelerators, suspending agents, salts or buffers for osmotic pressure control, coloring agents, spices, stabilizers, preservatives, preservatives or Other commercially available adjuvants may be further included.

Hereinafter, the present invention will be described in more detail through examples and the like. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

[Example] Preparation of small particle number

1 kg of Korean mistletoe leaves, noni fruit, sprouted barley, ginseng, and aronia fruit were each put in the ultra-vacuum microwave device shown in FIG. did. At this time, the temperature used was 30 degrees and the extraction time was 5 hours.

The frequency (Hz) of the number of small particles thus prepared was measured and shown in Table 1 and FIG. 1 below. Specifically, the Hz of the number of small particles was measured using a nuclear magnetic resonance spectrometer (NMR Spectrometer) at the Seobu Center, Seoul, Korea Research Institute for Basic Science. The O17-NMR half width of water was measured using an NMR-500 instrument.

extract raw material Frequency ( ¹⁷ O NMR linewidth) Example 1 mistletoe 45.411 Hz Example 2 noni fruit 47.260 Hz Example 3 sprout barley 44.649 Hz Example 4 aronia 48.279 Hz Comparative Example 1 Ginseng 60.259 Hz

As can be seen in Table 1, it can be seen that all of the small particle numbers of Examples 1 to 4 extracted from mistletoe, noni fruit, sprouted barley, and aronia excluding ginseng exhibited 50 Hz or less.

[Test Example 1] Measurement of antioxidant power of small particles

The antioxidant power of the small particles of Examples 2 to 4 and Comparative Example 1 was measured using the DPPH method. Specifically, the degree of reduction of 1,1-diphenyl-2-picryl hydrazyl (DPPH·), a stable free radical, by reaction with the sample was measured with a spectrophotometer (517 nm), and the antioxidant activity was converted and shown in FIG. . Vitamin C (0.1 mM) was used as a control.

As can be seen from Figure 2, compared to vitamin C used as a control, Example 2 (noni) was 95%, Example 4 (aronia) was 75%, Example 3 (barley sprout) was 74%, Comparative Example 1 ( ginseng) showed 32%, confirming that it has strong antioxidant power.

[Test Example 2] Efficacy evaluation of the number of small particles on AMPK expression

에서 12시간 이상 반응시킨 후 씻어주었다. 그 후 이차항체로 goat anti-mouse IgG HRP conjugate 항체 (Cell Signaling Technology, MA, USA)를 1:5,000의 농도로 희석하여 1시간 반응하였다. 마지막으로 Enhanced chemiluminescent(ECL) 용액(Thermo Fisher Scientific, MA, USA)을 이용하여 X-ray film에 노출한 후 TM-300E 현상기(Taeahn machinery, Incheon, Korea) 로 현상하였다. 이렇게 하여 얻은 결과는 도 3에 나타내었다(도 3의 NT는 처리하지 않은 대조군, UA는 ursolic acid 50uM).The degree of activation of AMP-dependent protein kinase (AMPK) in the number of small particles was measured using Western blotting. 5 × 10 ⁴ C2C12 cells were seeded in a 6 well plate (Nunc, USA) and cultured in a growth medium until 80-90% confluency was achieved, then changed to a differentiation medium to differentiate into muscle cells, and then used for the experiment. . The fermented product was treated at concentrations of 50 μg/mL and 100 μg/mL for 2 hours, and then the cells were recovered using a lysis buffer. , USA) was used for protein quantification. 30 μg/well of each quantified sample was loaded on a gel, and SDS-PAGE electrophoresis was performed at a voltage of 145 V for 1 hour and 20 minutes, and the gel was transferred to a Nitrocellulose filter at 200 mA for 2 hours. After blocking with 5% skim milk (Wako, USA) for 30 minutes, phospho-AMPKα (Thr172) antibody and B-actin antibody purchased from Cell Signaling Technology (MA, USA) were diluted to a concentration of 1:1,000, respectively.

After reacting for more than 12 hours, it was washed. Then, as a secondary antibody, goat anti-mouse IgG HRP conjugate antibody (Cell Signaling Technology, MA, USA) was diluted to a concentration of 1:5,000 and reacted for 1 hour. Finally, after exposure to the X-ray film using an enhanced chemiluminescent (ECL) solution (Thermo Fisher Scientific, MA, USA), it was developed with a TM-300E developer (Taeahn machinery, Incheon, Korea). The results obtained in this way are shown in FIG. 3 (NT is an untreated control in FIG. 3, UA is ursolic acid 50uM).

As can be confirmed from FIG. 3 , it was confirmed that Examples 1 to 3 had comparable activation ability when compared with UA (ursolic acid 50uM), which is known as the strongest agonist of AMPK.

[Test Example 3] Measurement of rust formation inhibitory ability of small particles

After immersing the clip in Example 1 and tap water, it was left at 25° C. for 2 weeks, and the results are shown in FIG. 4 . As can be seen from Figure 4, in the case of Example 1, rust formation was significantly suppressed, so rust could not be observed, whereas in the case of tap water, it was found that rust was clearly formed with the naked eye.

[Test Example 4] Measurement of plant growth promoting effect

After sowing barley seeds in the same area in the same amount, tap water, purified water, and the number of small particles (0.1%) of Example 3 were side-fertilized 2-3 times a day for 6 days. Barley sprouts grown after 6 days are shown in FIG. 5 . As can be seen from FIG. 5 , it was found that the growth was remarkable in Example 3 enough to be easily distinguished with the naked eye, and as a result of measuring the weight of the actually grown barley sprouts, tap water and purified water in Example 3 to 35% or more.

After filling a 2L pot with general soil and seedling medium, radish seeds were sown. The radishes so sown were fertilized with irrigation three times for 30 days with the same amount of the general water and the small particle number stock solution of Example 3. The radishes grown after 30 days are shown in FIG. 6 , and their weights were measured and shown in Table 2.

extract raw material Frequency ( ¹⁷ O NMR linewidth) Example 1 mistletoe 45.411 Hz Example 2 noni fruit 47.260 Hz Example 3 sprout barley 44.649 Hz Example 4 aronia 48.279 Hz Comparative Example 1 Ginseng 60.259 Hz

Referring to FIG. 6, although they were not easily distinguished in appearance, as shown in Table 2, the weight of the radish increased by 26% or more compared to the general product in Example 3. It is determined that this is because the yeol radish grown in Example 3 has a dense tissue.

Claims (10)

As the number of small particles,
The number of small particles is the number of small particles of 50 Hz or less of water. According to claim 1,
The number of small particles is characterized in that extracted by the ultra-vacuum microwave extraction method, the number of small particles. 3. The method of claim 2,
The number of small particles is extracted from vegetables or fruits, the number of small particles. A composition for activating an AMP-dependent protein kinase (AMPK) comprising the number of small particles of any one of claims 1 to 3. An antioxidant composition comprising the number of small particles of any one of claims 1 to 3. A composition for inhibiting rust formation comprising the small number of particles according to any one of claims 1 to 3. A composition for plant growth comprising the number of small particles of any one of claims 1 to 3. A food composition comprising the number of small particles of any one of claims 1 to 3. A cosmetic composition comprising the small number of particles of any one of claims 1 to 3. A pharmaceutical composition comprising the number of small particles of any one of claims 1 to 3.

Images