KR20220083345A - A Composition For Preventing Hair Loss Comprising Gaetbangpung Extract Using Hexane Or Fraction Thereof - Google Patents

Abstract

The present invention provides a composition for preventing hair loss or for improving hair growth, hair growth, or hair comprising the hexane extract of Gaetbangpung or a fraction thereof as an active ingredient. The composition for preventing hair loss or improving hair growth, hair growth, or hair of the present invention promotes the proliferation of human dermal papilla cells and has excellent beta-catenin activation efficacy, so it can exhibit effects such as preventing hair loss or improving hair growth, hair growth, and hair.

Description

A composition for preventing hair loss comprising a hexane extract or a fraction thereof of Gaetbangpung {A Composition For Preventing Hair Loss Comprising Gaetbangpung Extract Using Hexane Or Fraction Thereof}

The present invention relates to a composition for preventing hair loss comprising a hexane extract of Gaetbangpung or a fraction thereof.

For modern people, human hair is recognized for its importance in terms of aesthetics and sexuality, in addition to protecting the head and scalp. It greatly affects the hair follicles, leading to hair loss as well as hair loss.

The exact cause of hair loss is not yet known, but in general, degeneration of scalp cells due to aging including the influence of genetic factors and hormones, lack of nutrients supplied to the hair roots, and oxygen supply due to excessive excretion of dead skin cells/sebum from scalp cells Lack of skin care, stress, obesity, peroxide, reduced scalp function due to bacteria, etc., and clogging of pores.

There are several differences between male and female hair loss. In the case of androgenetic alopecia, more than 95% are caused by excessive secretion of male hormones based on genetic factors. It is converted to a substance called dihydro testosterone (DHT, Dihydro testosterone) by a specific enzyme, and when the concentration of DHT in the body is relatively high, the shrinkage of the hair follicle is promoted and hair loss proceeds quickly. Although DHT acts as a major cause in female alopecia, fortunately, female hormones are secreted in women, and it is known that DHT relatively inhibits the destructive action of hair follicles.

Currently, numerous hair loss prevention-related products such as hair loss prevention agents, hair growth promoters, and hair loss treatment agents are on the market, and representative hair loss prevention agents include Propecia and Minoxidil approved by the US FDA. However, since hair loss prevention and hair loss treatment by chemical drugs can cause side effects due to long-term use, various compositions for preventing hair loss using natural products are being developed to improve the side effects caused by these chemical drugs. Consumer response to the material is also increasing.

As an example of a cosmetic composition for preventing hair loss using a natural product, Patent Document 1 is for preventing itching and hair loss containing 12 herbal medicines of ginseng, sauerkraut, arborvitae, astragalus, ginseng, dandelion, licorice, doin, angelica, ginkgo leaf, health and safflower Disclosed is a cosmetic composition. However, in the case of a composition for preventing hair loss using these various herbal materials, it is limited in that it has not been able to identify a material particularly effective for preventing hair loss.

On the other hand, Gaetbangpung (Glehnia littoralis Fr. Schm.) is a perennial herb belonging to the Umbelliferae family, and is a medicinal plant distributed in Japan, Manchuria, and China. Its roots are called northern ginseng, empty wind, or liberation wind. It has been traditionally used as an expectorant and digestive medicine.

The present inventors confirmed that the Gaetbangpung extract using hexane as an extraction solvent promotes the proliferation of human dermal papilla cells, thereby preventing hair loss or improving hair growth, hair growth, and wool, and completed the present invention.

Korean Patent Registration No. 10-0640220

An object of the present invention is to provide a composition for preventing hair loss or improving hair growth, hair growth, and hair using a natural product.

One aspect of the present invention provides a composition for preventing hair loss comprising a hexane extract of Gaetbangpung or a fraction thereof as an active ingredient.

Another aspect of the present invention provides a composition for improving hair growth, hair growth, or hair comprising the hexane extract of Gaetbangpung or a fraction thereof as an active ingredient.

Since the composition for preventing hair loss of the present invention promotes the proliferation of human dermal papilla cells and has excellent beta-catenin activation efficacy, it can exhibit effects such as preventing hair loss or improving hair growth, hair growth, and hair growth.

1 shows the proliferation efficacy of human dermal papilla cells of Gaetbangpung extract for each solvent.
Figure 2 shows the effect of improving beta-catenin expression of Gaetbangpung extract for each solvent.

Hereinafter, the present invention will be described in more detail to help the understanding of the present invention.

The terms or words used in the present specification and claims should not be construed as being limited to ordinary or dictionary meanings, and the inventor may properly define the concept of the term in order to best describe his invention. Based on the principle that there is, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

One aspect of the present invention provides a composition for preventing hair loss comprising a hexane extract of Gaetbangpung or a fraction thereof as an active ingredient.

In the present invention, 'hair loss' refers to a state in which there is no hair in a region where hair should normally exist, and specifically refers to the loss of hair (thick and dark hair) of the scalp.

In the present invention, 'hair' may be used to include all parts of the body having hair roots and follicles, hair and eyelashes, eyebrows, beard, armpits, pubic hair, and hair follicles throughout the body.

Another aspect of the present invention provides a composition for improving hair growth, hair growth, or hair comprising the hexane extract of Gaetbangpung or a fraction thereof as an active ingredient.

In the present invention, 'hair growth' refers to hair growth in hair follicles.

In the present invention, 'hair growth' and 'wool' refer to lengthening of hair (ie, hair growth), and may be used in a similar sense to 'hair growth'.

In the present invention, the term 'extract' refers to a material extracted from Gaetbangpung by any method, and is used in the sense of including, without limitation, the extracted extract, the concentrate obtainable therefrom, and the dry matter and powder of the concentrate. The extract may be obtained by extraction from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as cultivated or commercially available ones. In addition, in the present invention, the extract includes a fraction obtained by additionally fractionating the extract.

In the present invention, when extracting from a raw material to obtain an extract, it is characterized in that the extract is obtained by a solvent extraction method using hexane as an extraction solvent.

In the present invention, hexane is a transparent, colorless liquid, and is widely used as an organic solvent because of its stable bonding and non-polarity. In addition, hexane is also used as the name of normal hexane (n-hexane).

The extraction of the Gaetbangpung may be performed at 0-100 °C, preferably at 10-50 °C, more preferably at 10-35 °C. In the case of the extract obtained under the above conditions, hair loss prevention or hair growth, hair growth, or wool effect may be more excellent.

In addition, the extraction of Gaetbangpung may be performed at 10-500 bar, preferably at 100-500 bar, more preferably at 300-500 bar. In the case of the extract obtained under the above conditions, hair loss prevention or hair growth, hair growth, or wool effect may be more excellent.

In addition, the extraction of Gaetbangpung may be performed for 12 to 48 hours, preferably 15 to 30 hours, more preferably 18 to 24 hours. In the case of the extract obtained under the above conditions, hair loss prevention or hair growth, hair growth, or wool effect may be more excellent.

Gaetbangpung extract of the present invention may be a dried product obtained by drying the extract obtained by the above extraction at 10 to 60 ℃, preferably at 15 to 50 ℃, more preferably at 20 to 40 ℃.

In order to obtain a specific active ingredient having a high anti-hair loss effect from the Gaetbangpung hexane extract, a fraction obtained by additionally performing a conventional fractionation process may be used. For example, a fraction obtained by passing an extract obtained according to the above extraction through an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity) The active fractions obtained through various purification methods additionally carried out such as separation are also included in the extract of the present invention. By separating the extract in which several components are mixed according to the properties of the active ingredient through concentration gradient column chromatography, etc., it is possible to prepare a specific active fraction having a higher anti-hair loss effect.

The chromatography may be, for example, at least one selected from the group consisting of column chromatography, high performance liquid chromatography (HPLC), ion exchange chromatography, affinity chromatography, and size exclusion chromatography.

The column chromatography is performed by column chromatography using a filler selected from the group consisting of silica gel, Sephadex, LH-20, ODS gel, RP-18, polyamide, Toyopearl and XAD resin to separate the active fraction and purification, and column chromatography may be performed several times by selecting an appropriate filler as needed, but is not limited thereto. In using the chromatography, the elution solvent, elution rate, and elution time may be applied to a solvent, rate, or time generally used in the art.

In addition, in order to obtain a specific active ingredient having a high anti-hair loss effect, a fraction obtained by fractionating the hexane extract of Gaetbangpung with an organic solvent may be used. The organic solvent is alcohol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene, acetone, acetonitrile, and mixtures thereof. It may be any one selected from the group consisting of, and preferably, the organic solvent may include at least one selected from the group consisting of butanol and methylene chloride, but is not limited thereto.

The composition for preventing hair loss or hair growth, hair growth, or hair improvement of the present invention may further include a solvent such as DMSO in addition to the Gaetbangpung hexane extract or a fraction thereof.

The active ingredient of the composition for preventing hair loss, the hexane extract of Gaetbangpung or a fraction thereof, may be included in the composition at a concentration of 2 to 25 μg/ml, or 6.25 to 25 μg/ml, or 10 to 18 μg/ml.

The composition for preventing hair loss or the hair growth, hair growth, or hair improvement composition of the present invention may be a cosmetic composition for preventing hair loss or improving hair growth, hair growth, or hair, and the cosmetic composition of the present invention comprises the Gaetbangpung extract or a fraction thereof. Based on the total weight, 0.0001 to 10% by weight, or 0.001 to 5% by weight, or 0.01 to 3% by weight, or 0.1 to 2% by weight, or 0.5 to 1.5% by weight may be included.

The cosmetic composition of the present invention may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form, for example, but not limited thereto, may include, but are not limited to, the form of lotion, cream, lotion, bathing agent, and the like.

Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited, and examples thereof include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin, and the like.

When the composition of the present invention is prepared as a cosmetic composition, the composition of the present invention may include not only the Gaetbangpung hexane extract or a fraction thereof, but also ingredients commonly used in cosmetic compositions, for example, antioxidants, stabilizers, solubilizers , conventional adjuvants such as vitamins, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

The cosmetic composition of the present invention may be used alone or in overlapping application, or may be used in overlapping application with other cosmetic compositions other than the present invention. In addition, the cosmetic composition of the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

The composition for preventing hair loss or hair growth, hair growth, or hair improvement composition of the present invention may be a quasi-drug composition for preventing hair loss or improving hair growth, hair growth, or hair, and the quasi-drug composition of the present invention is a composition comprising the Gaetbangpung extract or a fraction thereof. Based on the total weight, 0.0001 to 10% by weight, or 0.001 to 5% by weight, or 0.01 to 3% by weight, or 0.1 to 2% by weight, or 0.5 to 1.5% by weight may be included.

When the Gaetbangpung extract or a fraction thereof of the present invention is used as an active ingredient in a quasi-drug composition, the extract exhibiting the effect of preventing hair loss or improving hair growth, hair growth, or hair may be added as it is, or used together with other quasi-drugs or quasi-drug ingredients, It may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use.

The quasi-drug composition of the present invention is not particularly limited in its formulation, and it can be formulated in various forms in the form of quasi-drugs known in the art to exhibit an effect of preventing hair loss or improving hair growth, hair growth, or hair. The formulated quasi-drug may be an ointment, a cream, a gel, a liquid, an emulsion, a patch, or a spray, but is not limited thereto. Specifically, hair tonic, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair conditioner, hair shampoo, hair conditioner, hair pack, hair treatment, eyebrow hair growth agent, eyelash hair growth agent, eyelash nutrition agent, pet shampoo, pet There are animal rinses, hand sanitizers, detergents, soaps, disinfectants, wet wipes, masks, ointments, patches, or filter fillers, and includes all quasi-drugs in the ordinary sense.

In addition, in each formulation, the quasi-drug composition for preventing hair loss or improving hair growth, hair growth, or hair may be formulated by selecting other ingredients arbitrarily according to the formulation or purpose of use of other quasi-drugs. The mixing amount of the active ingredient may be suitably determined depending on the intended use, and may include, for example, conventional auxiliary agents such as thickeners, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers.

Hereinafter, the present invention will be described in detail by way of preparation examples and examples.

However, the following Preparation Examples and Examples are only for illustrating the present invention, and the content of the present invention is not limited by the following Preparation Examples and Examples.

Example

Preparation Example 1 - Preparation of Gaetbangpung Extract

To 10 g of Glehnia littoralis powder, hexane, methylene chloride, ethyl acetate, butanol, and water were added at 10 times the weight, respectively, and extracted at 25° C. for 24 hours. , water) extracts were obtained. The obtained Gaetbangpung extracts for each solvent were concentrated under reduced pressure and dried to obtain about 3.51 g of each Gaetbangpung extract powder.

Experimental Example 1

Gaetbangpung extract obtained by extraction with each solvent (hexane, methylene chloride, ethyl acetate, butanol, water) in Preparation Example 1 for each concentration (1.5625, 3.125, 6.25, 12.5, 25, 50, 100 μg/ml) By treatment with human dermal papilla cells, the cell proliferation efficacy was evaluated.

Cell proliferation efficacy was measured by the MTT reduction (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction method) method, When the extract was treated, the degree of cell proliferation was calculated as a percentage and shown in Table 1 and FIG. 1 below.

density
(μg/ml) DPC cell proliferation Hex MC EtOAc BuOH DW 0 102.9537 104.3054 101.602 101.8273 97.32163 1.5625 95.96994 94.61825 93.49184 93.49184 95.06881 3.125 111.9649 102.5031 90.3379 92.59072 96.19522 6.25 121.652 111.5144 112.4155 102.7284 110.8385 12.5 143.5043 116.02 105.657 109.9374 113.5419 25 111.2891 104.7559 100.9261 99.57444 91.46431 50 68.03503 103.8548 92.14015 93.9424 91.68959 100 35.59448 75.01875 104.3054 103.6295 95.29409

From the above results, it can be confirmed that the Gaetbangpung extract using hexane has a significantly superior proliferative effect on dermal papilla cells at a concentration of 1.5625 to 25 μg/ml, particularly 12.5 μg/ml, compared to the Gaetbangpung extract using other solvents.

Therefore, since the Gaetbangpung extract using hexane of the present invention has excellent cell proliferation efficacy, it can also be expected that it is excellent in preventing hair loss or improving hair growth, hair growth, or hair.

Experimental Example 2

After treatment with DHT (dihydrotestosterone) causing hair loss in all groups except for the untreated group, Gaetbangpung extract obtained by extraction with each solvent (hexane, methylene chloride, ethyl acetate, butanol, water) in Preparation Example 1 Treated with 12.5 ㎍ / ㎖, and treated with 100 μM minoxidil as a positive control, the beta-catenin expression level was evaluated. Beta-catenin is a protein that plays an important role in hair growth as a representative signal transduction material that decreases during the hair loss process.

The beta-catenin expression level was measured using Biovision's beta-catenin ELISA Kit according to the kit's protocol, and the beta-catenin expression level when the extract was treated by concentration compared to the negative control that was not treated with the extract was calculated as a percentage. It is shown in Table 2 and FIG. 2 below.

unprocessed DHT (100 nM) DHT (100 nM)
+
Minoxidil (100 μM) DHT (100nM) + Gaetbangpung extract (12.5 μg/ml) Hex MC DW EtOAc BuOH Beta-catenin expression level (%) 100 85.7143 115.7143 117.1429 95.7143 101.4286 98.5714 95.7143

From the above results, it can be seen that in the case of Gaetbangpung extract using hexane, beta-catenin is much more expressed than in Gaetbangpung extract using other solvents. In addition, in the case of the minoxidil-treated group as a positive control group, the expression of beta-catenin is significantly increased compared to the negative control group. can check that

Therefore, the Gaetbangpung extract using hexane of the present invention is excellent in the activating effect of beta-catenin, so it can also be expected that it is excellent in preventing hair loss or improving hair growth, hair growth, or hair.

Preparation Example 2 - Preparation of a cosmetic composition comprising Gaetbangpung hexane extract

2-1. Preparation of Essence

Essence was prepared according to the content (parts by weight) shown in Table 3 below using the Gaetbangpung extract using hexane of Preparation Example 1.

Raw material Content (parts by weight) triethanolamine 0.25 Carboxyvinyl Polymer 0.22 glycerin 4 1,3-butylene glycol 2 Gaetbangpung Hexane Extract 1.5 beeswax 0.5 cetostearyl alcohol One Glyceryl Monostearate One squalene 4 Purified water remaining amount Sum 100

2-2. Manufacture of softened lotion

Softening lotion containing Gaetbangpung extract using hexane of Preparation Example 1 as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDIT 0.05 allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric Acid 0.01 sodium citrate 0.02 Glyceres-26 1.00 arbutin 2.00 PEG-40 Hydrogenated Castor Oil 1.00 ethanol 30.00 Gaetbangpung Hexane Extract 0.5 coloring agent a very small amount flavoring agent a very small amount Purified water remaining amount Sum 100

2-3. Preparation of nourishing cream

Nutrient cream containing Gaetbangpung extract using hexane of Preparation Example 1 as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic Acid 2.0 Polysorbate 60 1.5 Lipophilic Glyceryl Stearate 2.0 Sorbitan sesquioleate 1.5 cetearyl alcohol 3.0 mineral oil 4.0 squalene 3.8 Gaetbangpung Hexane Extract 1.5 vegetable oil 1.8 dimethicone 0.4 Dipotassium glycyrrhizate a very small amount allantoin a very small amount Sodium Hyaluronate a very small amount tocopheryl acetate appropriate amount triethanolamine appropriate amount flavoring agent appropriate amount Purified water remaining amount Sum 100

2-4. manufacture of lotions

A lotion containing the Gaetbangpung extract using hexane of Preparation Example 1 as an active ingredient was prepared as shown in Table 6 below.

Raw material Content (parts by weight) cetostearyl alcohol 1.6 stearic acid 1.4 lipophilic monostearate glycerin 1.8 PEG-100 Stearate 2.6 Sorbital Sesquioleate 0.6 squalene 4.8 macadaia oil 2 jojoba oil 2 tocopherol acetate 0.4 Methylpolysiloxane 0.2 tocopherol acetate 0.4 1,3-butylene glycol 4 Xanthan Gum 0.1 glycerin 4 d-pandenol 0.15 Gaetbangpung Hexane Extract 1.0 allantoin 0.1 Carbomer (2% aq. Sol) 4 triethanolamine 0.15 ethanol 3 Purified water remaining amount Sum 100

Preparation Example 3. Preparation of shampoo for preventing hair loss comprising Gaetbangpung hexane extract

A shampoo for preventing hair loss including the Gaetbangpung extract using hexane of Preparation Example 1 was prepared according to the composition of Table 7 below.

Raw material Content (parts by weight) Gaetbangpung Hexane Extract 5 Disodium EDIT 0.03 Polyquaternium-10 0.1 Ammonium Lauryl Sulfate 40.5 Ammonium Laureth Sulfate 15.5 Cocamidopropyl Betaine 6 propylene glycol 2 Sodium Xylenesulfonate 2 Glycol Distearate 3 Cocamide A. One Lauramide A. One salicylic acid 0.13 Simethicone 3.0 antiseptic appropriate amount flavoring agent appropriate amount Purified water remaining amount Sum 100.0

Claims (9)

A composition for preventing hair loss comprising the hexane extract of Gaetbangpung or a fraction thereof as an active ingredient. The method according to claim 1,
Extraction of Gaetbangpung composition is carried out at 0 ~ 100 ℃. The method according to claim 1,
Extraction of Gaetbangpung composition is carried out at 10 ~ 500bar. The method according to claim 1,
The composition of Gaetbangpung extract is carried out for 12 to 48 hours. A composition for improving hair growth, hair growth, or hair comprising the hexane extract of Gaetbangpung or a fraction thereof as an active ingredient. 6. The method of claim 5,
Extraction of Gaetbangpung composition is carried out at 0 ~ 100 ℃. 6. The method of claim 5,
Extraction of Gaetbangpung composition is carried out at 10 ~ 500bar. 6. The method of claim 5,
The composition of Gaetbangpung extract is carried out for 12 to 48 hours. 9. The method according to any one of claims 1 to 8,
A composition that is a cosmetic composition or a quasi-drug composition.

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