KR102301603B1 - Composition Improving Wrinkles Of Skin Comprising Ginseng Leaf Extract - Google Patents

Abstract

The present invention comprises the steps of obtaining an alcohol extract of ginseng leaves; and removing the ethyl acetate fraction from the alcohol extract; provides a cosmetic composition for improving skin wrinkles, comprising a ginseng leaf extract obtained by a manufacturing method comprising a. The cosmetic composition of the present invention has excellent collagen synthesis ability, low cytotoxicity, and excellent formulation stability, so that it can be used for improving skin wrinkles, improving skin elasticity, or regenerating skin.

Description

Composition for improving skin wrinkles comprising ginseng leaf extract {Composition Improving Wrinkles Of Skin Comprising Ginseng Leaf Extract}

The present invention relates to a composition for skin wrinkle improvement comprising a ginseng leaf extract and a method for preparing the same.

As the skin ages, the aging phenomenon intensifies. Skin exposed to sunlight for a long period of time When exposed, changes occur in the epidermis and dermis tissue, and wrinkles increase, the skin thickens and loses elasticity, and becomes rough and spots. Since such skin aging can be delayed by promoting the synthesis of collagen, research to develop a material that is safe for the body while enhancing the synthesis of collagen is being actively conducted.

Collagen is a major component of the extracellular matrix and is a major matrix protein produced by fibroblasts of the skin. In addition, it is an important protein accounting for about 30% of the total weight of biological proteins and has a strong triple helix structure. Collagen forms most of the organic matter of the skin, tendons, bones and teeth, and the content is particularly high in bones and skin. It exists as a fibrous inclusion body in most other body structures.

The main functions of collagen are known as mechanical firmness of the skin, resistance of connective tissue and tissue bonding, support of cell adhesion, and induction of cell division and differentiation (in the case of growth of an organism or wound healing). In addition, in recent years, extensive research on skin aging has been made, revealing the important function of collagen in the skin. For example, collagen decreases with age and photoaging by UV irradiation, which is known to be closely related to the formation of wrinkles in the skin. In addition, collagen plays an important role in wound healing, and promotes the synthesis of collagen in the damaged epithelium, so that the wound can be repaired quickly and without scarring. As described above, when collagen metabolism is activated by promoting collagen synthesis, the components of the dermal matrix are increased, so that the effects of wrinkle improvement, elasticity enhancement, skin strengthening and wound healing can be expected.

In order to obtain this effect, conventionally, products containing collagen in cosmetics have been released to take advantage of the skin wrinkle improvement effect of collagen. However, these cosmetics apply collagen to the skin surface. Since an improvement action cannot be expected, it cannot be said that it is an essential skin function improvement.

Therefore, interest in substances capable of promoting collagen synthesis in the skin itself has increased, and conventionally, as substances capable of promoting collagen synthesis, retinoic acid, transforming growth factor (TGF), chlorella extract, and the like have been used. is known However, retinoic acid is unstable and there is a limitation in the amount of use due to stability problems such as irritation and redness when applied to the skin, and the effect of chlorella extract and the like is insignificant. Therefore, there is a demand for the development of a new collagen synthesis promoter that is safe for the living body and has a higher effect than the existing collagen synthesis promoter.

In order to suppress such skin wrinkles or to alleviate the progression rate, cosmetics or skin protectants containing various skin protecting ingredients such as retinol, retinoid, vitamin C, flavonoids, and tocopherol have been developed, but such cosmetics or skin protectants In the final formulation, the potency is lowered, the efficacy is mild, and the skin irritation is severe. Therefore, there is still a need for the development of a substance that can improve skin aging by reducing skin wrinkles.

Ginseng leaves are known to have as high a ginsenoside content as ginseng roots, but research on overall ginseng leaf extracts, including specific components, extraction methods and efficacy for extracting them, is insufficient. The present inventors have found that an extract having excellent collagen synthesis ability can be obtained by efficiently removing stability-inhibiting substances including pigment components such as chlorophyll contained in large amounts in ginseng leaves, and completed the present invention.

Korean Patent Publication No. 2004-0026148

An object of the present invention is to provide a cosmetic composition comprising a ginseng leaf extract that can be used for improving skin wrinkles, improving skin elasticity, or regenerating skin due to its excellent collagen synthesis ability.

An object of the present invention is to provide a method for preparing the ginseng leaf extract.

One aspect of the present invention comprises the steps of obtaining an alcohol extract of ginseng leaves; and removing the ethyl acetate fraction from the alcohol extract; provides a cosmetic composition for improving skin wrinkles, comprising a ginseng leaf extract obtained by a manufacturing method comprising a.

One aspect of the present invention comprises the steps of obtaining an alcohol extract of ginseng leaves; And removing the ethyl acetate fraction from the alcohol extract; provides a cosmetic composition for improving skin elasticity comprising a ginseng leaf extract obtained by a manufacturing method comprising a.

One aspect of the present invention comprises the steps of obtaining an alcohol extract of ginseng leaves; And removing the ethyl acetate fraction from the alcohol extract; provides a cosmetic composition for skin regeneration comprising a ginseng leaf extract obtained by a manufacturing method comprising a.

One aspect of the present invention comprises the steps of obtaining an alcohol extract of ginseng leaves; and removing the ethyl acetate fraction from the alcohol extract.

The cosmetic composition of the present invention has excellent collagen synthesis ability, low cytotoxicity, and excellent formulation stability, so that it can be used for improving skin wrinkles, improving skin elasticity, or regenerating skin.

1 shows the increase rate of procollagen type I synthesis according to each treatment concentration of the extracts of Example 1 and Comparative Examples 1 to 6 as an embodiment of the present invention.
2 is a photograph confirming whether a precipitate is formed after centrifugation is performed on the extracts of Example 1 and Comparative Example 4 as an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail to help the understanding of the present invention.

The terms or words used in the present specification and claims should not be construed as being limited to their ordinary or dictionary meanings, and the inventor may properly define the concept of the term in order to best describe his invention. It should be interpreted as meaning and concept consistent with the technical idea of the present invention based on the principle that there is.

1. Preparation of ginseng leaf extract

One aspect of the present invention comprises the steps of obtaining an alcohol extract of ginseng leaves; and removing the ethyl acetate fraction from the alcohol extract.

In the present invention, the term 'ginseng leaf' may be used to include all of ginseng sprouts, ginseng leaves, and ginseng stems. Accordingly, the ginseng leaves may include at least one selected from the group consisting of ginseng sprouts, ginseng leaves and ginseng stems.

In the present invention, the term 'extract' refers to a material extracted from a raw material by any method, and is used in the meaning of including, without limitation, the extracted extract, the concentrate obtainable therefrom, the dried product and the powder of the concentrate.

The extract may be obtained by extraction from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as cultivated or commercially available ones.

When the extract is obtained by extracting from the raw material, as the extraction method, all conventional known extraction methods such as solvent extraction, ultrasonic extraction, filtration, and reflux extraction may be used, and preferably prepared by using a solvent extraction method or reflux extraction method can do. The extraction process may be repeated several times, and thereafter, a step such as concentration or freeze-drying may be additionally performed. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrate, and after freeze-drying the concentrate, a high concentration extract powder can be prepared by using a grinder. The extract also includes a fraction obtained by further fractionating the extract.

In the present invention, the alcohol used for alcohol extraction of the ginseng leaves may be ethanol or an aqueous ethanol solution. In addition, the ethanol aqueous solution is 5% (v / v) to 99% (v / v) ethanol aqueous solution, or 5% (v / v) to 90% (v / v) ethanol aqueous solution, or 25% (v / v) ) to 85% (v/v) aqueous ethanol solution, or 30% (v/v) to 80% (v/v) aqueous ethanol solution, or 60% (v/v) to 80% (v/v) aqueous ethanol solution However, the present invention is not limited thereto.

The alcohol may be added 1 to 20 times by weight relative to the ginseng leaves, preferably 3 to 18 times, and more preferably 5 to 15 times, but is not limited thereto. If the alcohol is added below the lower limit, that is, in an amount smaller than that of ginseng leaves by weight, the active ingredient to be extracted from the ginseng leaves may not be sufficiently extracted.

Extraction may be carried out at 0 °C to 100 °C, preferably at 10 °C to 50 °C, more preferably at 10 °C to 35 °C, but is not limited thereto. The extraction may be carried out for 12 hours to 36 hours, preferably for 15 hours to 30 hours, and more preferably for 20 hours to 25 hours, but is not limited thereto. The extraction may be performed 1 to 8 times, preferably 1 to 6 times, and more preferably 1 to 5 times, but is not limited thereto, and the extract obtained in each extraction It may be a single extract or a mixed extract of extracts obtained in each extraction.

Ginseng leaves, especially ginseng leaves, contain a large amount of substances that inhibit the stability of the extract, including pigments such as chlorophyll. In the present invention, all solvents are removed from the alcohol extract of the ginseng leaves to obtain a concentrate, After dissolving in purified water, ethyl acetate is added thereto to perform solvent fractionation, whereby stability-inhibiting substances including chlorophyll dissolved in ethyl acetate can be efficiently removed.

The ethyl acetate fraction may be prepared by a conventional method, such as using an ultrafiltration membrane having a constant molecular weight cut-off value or various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), and in particular, not limited

For example, the step of removing the ethyl acetate fraction may include: a) removing a solvent containing alcohol from the alcohol extract to obtain a concentrate; b) dissolving the concentrate in purified water; c) mixing ethyl acetate with the solution in a weight ratio of 20:1 to 2:1; and d) separating only the water layer from the result obtained in step c) to obtain a water fraction.

The weight ratio of the alcohol extract and ethyl acetate may be 20:1 to 2:1, or 15:1 to 3:1, or 10:1 to 4:1. By performing ethyl acetate fractionation by using the alcohol extract and ethyl acetate in the above weight ratio, substances that inhibit the stability of the extract can be selectively removed, and the content of the active ingredient in the extract can be further increased. Specifically, in the preparation of a cosmetic composition having skin wrinkle improvement, skin elasticity improvement or skin regeneration effect, only substances that inhibit the stability of the extract are effectively removed by adjusting the weight ratio of the alcohol extract and ethyl acetate as described above, and the skin wrinkle improvement , it is possible to increase the ratio of active ingredients having an effect of improving skin elasticity or regenerating the skin.

In addition, for example, the concentrate and purified water in step b) may form a solution by dissolving approximately the same amount of purified water as the solvent removed in step a) after administration.

2. Cosmetic composition comprising ginseng leaf extract

One aspect of the present invention comprises the steps of obtaining an alcohol extract of ginseng leaves; And removing the ethyl acetate fraction from the alcohol extract; provides a cosmetic composition comprising a ginseng leaf extract obtained by a manufacturing method comprising a.

In the present invention, the 'cosmetic composition' may be a composition used to improve skin wrinkles, improve skin elasticity, or regenerate skin.

The preparation method of the ginseng leaf extract is the same as that described in "1. Preparation of the ginseng leaf extract", so the description will be omitted.

The cosmetic composition of the present invention comprises 0.0001 to 10% by weight, or 0.001 to 5% by weight, or 0.01 to 3% by weight, or 0.1 to 2% by weight, or 0.5 to 1.5% by weight of the ginseng leaf extract based on the total weight of the cosmetic composition can do.

The cosmetic composition of the present invention may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form, for example, but not limited thereto, may include, but are not limited to, the form of a lotion, cream, lotion, bathing agent, and the like.

Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited, and examples thereof include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin, and the like.

The cosmetic composition of the present invention may include not only ginseng leaf extract, but also ingredients commonly used in cosmetic compositions, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. may include

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

The cosmetic composition of the present invention may be used alone or in overlapping application, or may be used in overlapping application with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent skin regeneration effect according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in detail by way of Preparation Examples and Examples.

However, the following Preparation Examples and Examples are only for illustrating the present invention, and the content of the present invention is not limited by the following Preparation Examples and Examples.

Example 1

1-1. Ethanol extraction of ginseng leaf stem

To 100 g of ginseng leaf stem powder (Korea Ginseng Corporation), a 70% (v/v) aqueous solution of ethanol was added so as to be 10 times larger than the original, extracted three times at room temperature for 8 hours, and then concentrated under reduced pressure to remove the ethanol in the extract to about 100 g An ethanol aqueous solution extract was obtained.

1-2. Removal of ethyl acetate fraction from ethanol extract

The extract was first filtered through a 400 mesh filter and then centrifuged at 6,000 rpm. After centrifugation, the solid was removed, and the remaining supernatant was second filtered with a 1um filter. After the filtrate was completely concentrated with a vacuum concentrator, about 3.2 g of the ginseng leaf stem concentrate obtained was dissolved in 96.8 g of purified water to prepare 100 g of a diluted ginseng leaf stem concentrate. 10 g of ethyl acetate was added to the diluted ginseng leaf stem concentrate so that the weight ratio of the diluted ginseng leaf stem concentrate and ethyl acetate was 10:1, and only the water layer was separated from the mixture and concentrated under reduced pressure to obtain the water fraction of Example 1. did.

Comparative Examples 1 to 4

During ethanol extraction of ginseng leaf stems, water, ethanol 30% (v/v) aqueous solution, and ethanol 50% (v/v) aqueous solution were respectively treated to the ginseng leaf stem powder to obtain an extract, and then the ethyl acetate fraction removal step Concentrate of the water extract (Comparative Example 1), ethanol 30% (v/v) aqueous solution extract (Comparative Example 2), ethanol 50% in the same manner as in Example 1, except that (v/v) a concentrate of an aqueous solution extract (Comparative Example 3) and a concentrate of an ethanol 70% (v/v) aqueous solution extract (Comparative Example 4) were prepared.

Comparative Example 5

1-1. Water extraction of ginseng leaf stem

Water was added to 100 g of ginseng leaf stem powder (Korea Ginseng Corporation) so as to be 10 times larger than the original, and extracted three times for 8 hours at room temperature to obtain a water extract.

1-2. Removal of ethyl acetate fraction from water extract

In the same manner as applied in Example 1, the ethyl acetate fraction was removed from the water extract to obtain a water fraction of Comparative Example 5.

Comparative Example 6

An ethanol extract of ginseng leaf stem was obtained in the same manner as in Example 1, and then, when the ethyl acetate fraction was removed from the ethanol extract, 3.2 g of the ginseng leaf stem concentrate was dissolved in 96.8 g of purified water to dilute the ginseng leaf stem concentrate. 100 g was prepared, and 100 g of ethyl acetate was added to the diluted ginseng leaf stem concentrate so that the weight ratio of the diluted ginseng leaf stem concentrate and ethyl acetate was 1:1, and only the water layer was recovered from the mixture, but the same fractionation process was followed. A water fraction of Comparative Example 6 was obtained in the same manner as in Example 1, except that it was carried out twice in total.

Experimental Example 1

Skin fibroblasts (ATCC, USA) ^{were aliquoted at 1x10 5 in} a 24-well plate, and 10% bovine serum was added to a DMEM:F12 mixed medium, and then cultured at 37° C., 5% CO _{2 in an} incubator. After 24 hours, Examples and Comparative Examples were treated at a concentration of 10 μg/ml or 100 μg/ml, respectively, and as a positive control, L-ascrobic acid was treated at a concentration of 100 μg/ml. After 24 hours of sample treatment, only the supernatant was separated and the increase or decrease of procollagen in the supernatant was observed using a procollagen type (I) ELISA kit (Takara, Japan).

The increase (%) of procollagen type I synthesis compared to the untreated group was calculated and is shown in Table 1 and FIG. 1 .

Throughput
(μg/ml) compared to the untreated group
Increased amount of procollagen type I synthesis (%) Example 1
(Ethanol 70% aqueous solution extraction + EA fraction removal. Extract: EA weight ratio = 10:1) 10 6.54 100 26.16 Comparative Example 1
(water extraction) 10 -3.86 100 -5.44 Comparative Example 2
(Ethanol 30% aqueous solution extraction) 10 0.67 100 -3.70 Comparative Example 3
(Ethanol 50% aqueous solution extraction) 10 3.20 100 2.70 Comparative Example 4
(Extraction of 70% ethanol solution) 10 -4.54 100 3.95 Comparative Example 5
(water extraction + EA fraction removal) 10 2.00 100 1.10 Comparative Example 6
(Ethanol 70% aqueous solution extraction + EA fraction removal. Extract: EA weight ratio = 1:1, fractionation process proceeded twice) 10 -1.05 100 1.37

As a result of confirming the collagen synthesis ability of Comparative Examples 1 to 6 and Example 1, it can be seen that the collagen synthesis ability of Examples is significantly improved compared to Comparative Examples.

In particular, as in Comparative Example 6, it can be seen that the increase in procollagen synthesis is not high even when the fractionation is repeated twice by applying almost the same weight ratio of the extract concentrate diluent and ethyl acetate. This is that, in the present invention, ethyl acetate is used for the purpose of removing stability-inhibiting substances including chlorophyll. When fractionation is repeatedly performed by adding more than a certain weight ratio to the dilution of the extract concentrate, ginsenosides contained in the extract, etc. It is believed that this is because the active ingredient is also removed.

In addition, after taking 100 ml of each of the extracts of Example 1 and Comparative Example 4, centrifugation was performed under 3000 rpm and 3 minutes conditions, the solvent was removed, and the result of separating only the precipitate was photographed and shown in FIG. 2 . From the above results, the extract of Example 1 (FIG. 2A) did not form a precipitate, whereas in Comparative Example 4 (FIG. 2B), a precipitate was formed. Therefore, according to Example 1, it can be confirmed that the stability-inhibiting substances including chlorophyll are removed and the stability of the raw material is improved, whereas in Comparative Example 4, this was not the case.

<Preparation Example 1> Preparation of a cosmetic composition

<1-1> Preparation of essence

Using the fraction of Example 1, an essence was prepared according to the content (parts by weight) shown in Table 2 below.

Furtherance Content (parts by weight) triethanolamine 0.25 Carboxyvinyl Polymer 0.22 glycerin 4 butylene glycol 2 Fraction of Example 1 1.5 beeswax 0.5 cetostearyl alcohol One Glyceryl Monostearate One squalene 4 Purified water appropriate amount

<1-2> Manufacture of softened lotion

Softening lotion containing the fraction of Example 1 as an active ingredient was prepared as shown in Table 3 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDIT 0.05 allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric Acid 0.01 sodium citrate 0.02 Glyceres-26 1.00 arbutin 2.00 PEG-40 Hydrogenated Castor Oil 1.00 ethanol 30.00 Fraction of Example 1 0.5 coloring agent a very small amount flavoring agent a very small amount Purified water remaining amount

<1-3> Preparation of nourishing cream

Nutrient cream containing the fraction of Example 1 as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic Acid 2.0 Polysorbate 60 1.5 Lipophilic Glyceryl Stearate 2.0 Sorbitan sesquioleate 1.5 cetearyl alcohol 3.0 mineral oil 4.0 squalene 3.8 Fraction of Example 1 1.5 vegetable oil 1.8 dimethicone 0.4 Dipotassium glycyrrhizate a very small amount allantoin a very small amount Sodium Hyaluronate a very small amount Tocopheryl Acetate appropriate amount triethanolamine appropriate amount flavoring agent appropriate amount Purified water remaining amount

<1-4> Preparation of lotion

A lotion containing the fractions of Example 1 as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) cetostearyl alcohol 1.6 stearic acid 1.4 lipophilic monostearate glycerin 1.8 PEG-100 Stearate 2.6 Sorbital Sesquioleate 0.6 squalene 4.8 macadaia oil 2 jojoba oil 2 tocopherol acetate 0.4 Methylpolysiloxane 0.2 tocopherol acetate 0.4 1,3-butylene glycol 4 Xanthan Gum 0.1 glycerin 4 d-pandenol 0.15 Fraction of Example 1 1.0 allantoin 0.1 Carbomer (2% aq. Sol) 4 triethanolamine 0.15 ethanol 3 Purified water appropriate amount

Claims (8)

A cosmetic composition for skin wrinkle improvement, comprising a water-soluble fraction of ginseng leaves obtained by mixing and fractionating an aqueous alcohol solution extract of ginseng leaves and ethyl acetate in a weight ratio of 10:1 and then separating the water layer. A cosmetic composition for improving skin elasticity, comprising a water-soluble fraction of ginseng leaves obtained by mixing and fractionating an aqueous alcohol solution extract of ginseng leaves and ethyl acetate in a weight ratio of 10:1, and then separating the water layer. A cosmetic composition for skin regeneration comprising a water-soluble fraction of ginseng leaves obtained by mixing and fractionating an aqueous alcohol solution extract of ginseng leaves and ethyl acetate in a weight ratio of 10:1 and then separating the water layer. 4. The method according to any one of claims 1 to 3,
The alcohol is ethanol or an aqueous ethanol solution,
The ethanol aqueous solution is 5% (v / v) to 99% (v / v) ethanol aqueous solution cosmetic composition. delete obtaining an alcohol aqueous solution extract of ginseng leaves; and
A method for producing a water-soluble fraction of ginseng leaves, comprising the step of mixing the alcohol aqueous solution extract and ethyl acetate in a weight ratio of 10:1 and then separating the water layer. 7. The method of claim 6,
The alcohol is ethanol or an aqueous ethanol solution,
The ethanol aqueous solution is 5% (v / v) to 99% (v / v) ethanol aqueous solution. delete

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