KR20220046804A - Cleaning composition that can be used simultaneously as a hair shampoo and body wash - Google Patents

Abstract

The present invention relates to a human body cleansing composition and, more specifically, to a cleansing composition capable of being used as hair shampoo and body wash by including an onion extract as an active ingredient. The onion extract of the present invention has excellent antioxidant, anti-inflammatory and skin regeneration effects and is a substance extracted from an edible natural product, thereby being very safe to the human body. Accordingly, the composition of the present invention including the onion extract as an active ingredient can be used in a product such as hair shampoo and body wash. Specifically, the present invention is advantageous in that the optimized amount that can indicate stability in pH levels and viscosity when the onion extract is applied, as a functional component, to a human body cleansing composition, is identified, and a specific mixing ratio for each component of a composition that has excellent effects in the evaluation of washing power, foaming power and formulation suitability is primally identified.

Description

A cleaning composition that can be used simultaneously as a hair shampoo and body wash {Cleaning composition that can be used simultaneously as a hair shampoo and body wash}

The present invention relates to a human body cleansing composition, and more particularly, to a cleansing composition comprising onion extract as an active ingredient, which can be used simultaneously as a body wash along with a hair shampoo.

As the economy develops and living standards improve, the desire for well-being or healing is increasing. Accordingly, the desire for skin care has also increased, and the demand for human body cleansing products with excellent functional effects such as moisturizing and whitening or aromatherapy effects as well as cleansing power is increasing.

Human body cleansing agents include surfactants, cleaning aids, and moisturizing agents, and proper mixing of these ingredients provides moisturizing, cleansing and foaming power to the product. Sulfate-type surfactants have been used as main surfactants for reasons such as excellent cleaning power, abundant foaming power, and low price. However, recently, skin irritation, human toxicity, environmental safety, etc. have become a problem.

The skin, hands, and scalp are the body organs that are in close contact with the external environment within the human body, and are very important organs that have biochemical and physical functions to protect the inside of the human body.

When these skin, hands, and scalp generate active oxygen in the body, the active oxygen attacks the cell membrane, destroying the cells, and further aging occurs in the skin, hands, and scalp that form wrinkles. When aging occurs in the hand, it becomes cracked, rough, and wrinkled, and even blood leaks through the cracked area. In addition, when aging occurs on the scalp, it may cause hair loss.

Therefore, it always keeps the skin, hands and scalp in a moist state, facilitates blood circulation, removes active oxygen generated in the body, protects cell membranes, and further delays the aging of the skin, hands and scalp through anti-inflammatory action. Of course, already damaged cells need to be reactivated by active metabolism to proliferate the cells.

Therefore, recently, a composition for human body cleansing containing a functional ingredient with little irritation to the skin has been developed, and as such functional ingredient, research on extracts from natural materials is being actively conducted.

On the other hand, it is speculated that onions are native to Western Asia or the Mediterranean coast, but it is not certain as no wild species have been found yet. The scales are somewhat different depending on the variety, but they have a flat, round or round shape, and have a thin membranous (translucent like thin paper) purplish-brown skin on the outside, and the scales on the inside are thick, layered, and have a spicy taste. it flies The leaves are hollow, cylindrical, dark green, dry when flowers bloom, and the lower part is made of thick scaly pieces. Varieties are divided into round and flat scaly stems, and red, yellow, and white scaly skins. Also, according to taste, it is divided into sweet onion and spicy onion. There are four types of onion varieties that are generally edible: yellow onion, red onion, white onion, and small onion. The scales of onions are mainly eaten for food, and the characteristic odor from the stems is due to compounds such as propyl disulfide and allyl sulfide. It physiologically promotes the secretion of digestive juices and has effects such as excitement, sweating, and diuresis. In addition, as the scales contain various vitamins and minerals such as calcium and phosphoric acid, they have the effect of removing harmful substances from the blood. The scales are often used in salads, soups, and meat dishes, and are used as spices in various dishes. The leaves contain 5,000 IU of vitamin A, 45 mg of vitamin C, 80 mg of calcium, 24 mg of magnesium, and 220 mg of potassium in 100 g.

Under this background, the present inventors have confirmed that the onion extract has excellent antioxidant, anti-inflammatory, and skin regeneration effects, and tried to develop an all-in-one hair & body wash cleaning composition that can clean the whole body using this as a main ingredient. As a result, the pH and viscosity stability of the detergent composition for each added content of onion extract that imparts functionality were checked to derive the optimal amount added. In addition, the present invention was completed by confirming through an experiment that the effect was excellent in the evaluation of cleansing power, foaming power, and formulation aptitude at a specific mixing ratio for each component in the preparation of a human body cleansing composition containing onion extract.

Korean Patent Publication No. 10-2017-0063365

Accordingly, it is an object of the present invention to provide an all-in-one human body cleansing composition capable of whole-body skin cleansing comprising onion extract as a functional main ingredient.

In order to achieve the object of the present invention as described above,

The present invention provides a human body cleansing composition comprising an onion extract as an active ingredient.

In one embodiment of the present invention, the onion extract is minced by mixing onion, onion peel, and onion root in a ratio of 7:2:1, and then hot water extraction is performed by adding 8 to 10 times water to the minced mixture, followed by filtration. It can be prepared through the step of freezing and drying.

In one embodiment of the present invention, the onion extract may have a total polyphenol content of 932.62±24.39 mg/100g, a total flavonoid content of 566.67±41.67 mg/100g, and a quercetin content of 63.78±1.96 mg/100g. .

In one embodiment of the present invention, the onion extract may have antioxidant activity, anti-inflammatory activity and skin regeneration activity.

In one embodiment of the present invention, the onion extract may be included in the composition in an amount of 0.1 to 0.5% by weight.

In one embodiment of the present invention, the extract may be included in an amount of 0.1 to 10% by weight relative to the total composition.

In one embodiment of the present invention, the human body cleansing composition is in addition to onion extract, glycerin, betaine, xanthan gum, panthenol, polyquaternium-51, polylysine, caprylic glycol, ethylhexylglycerin, butylene glycol Cole, Decyl Glucoside, Lauryl Glucoside, Coco-Glucoside, Sodium Laureth Sulfate, Sodium Cocoyl Glutamate, PEG-120 Methyl Glucose Dioleate, Apricot Seed Oil, Jojoba Seed Oil, Evening Primrose Oil, Tea Tree Leaf Oil, Orange Flower oil, sentient geranium oil, marjoram flower oil, chamomile flower oil, cypress oil, lavender oil, orange peel oil and jasmine flower oil may be further included.

In one embodiment of the present invention, the human body cleansing composition is 0.5% by weight of onion extract, 3% by weight of glycerin, 1% by weight of betaine, 0.5% by weight of xanthan gum, 0.5% by weight of panthenol, 0.5% by weight of polyquaternium-51 , Polylysine 0.25% by weight, caprylic glycol 0.25% by weight, ethylhexylglycerin 0.25% by weight, butylene glycol 0.25% by weight, decylglucoside 8% by weight, lauryl glucoside 8% by weight, coco-glucoside 8% by weight , sodium laureth sulfate 5% by weight, sodium cocoyl glutamate 5% by weight, PEG-120 methylglucose dioleate 2.5% by weight, apricot seed oil 0.338% by weight, jojoba seed oil 0.279% by weight, evening primrose oil 0.2490% by weight, Tea tree leaf oil 0.1000% by weight, orange flower oil 0.0075% by weight, sentient geranium oil 0.0075% by weight, marjoram flower oil 0.0075% by weight, chamomile flower oil 0.0045% by weight, cypress oil 0.0020% by weight, lavender oil 0.0020% by weight, It may contain 0.0020% by weight of orange peel oil and 0.0010% by weight of jasmine flower oil.

In one embodiment of the present invention, the composition can be used simultaneously as a hair shampoo and body wash.

The onion extract of the present invention has excellent antioxidant, anti-inflammatory, and skin regeneration effects, as well as a material extracted from edible natural food and has very safe properties for the human body. The composition of the present invention containing it as an active ingredient is a hair shampoo and products such as body wash. In particular, when the onion extract as a functional ingredient is applied to the human body cleansing composition, the optimal amount of addition that can show pH and viscosity stability is identified, and the specific mixing ratio for each component of the composition, which is effective in evaluation of detergency, foaming power, and formulation aptitude, is determined. There is an advantage identified for the first time.

1 is an analysis of the quercetin content of onion extract using HPLC.
Figure 2 shows the DPPH scavenging ability according to the treatment for each concentration of the onion extract of the present invention.
Figure 3 shows the survival rate of Raw264.7 cells according to the treatment by concentration of the onion extract of the present invention.
Figure 4 shows the NO production inhibitory ability according to the treatment for each concentration of the onion extract of the present invention.
Figure 5 shows the inhibitory ability of inflammatory cytokines according to the treatment by concentration of the onion extract of the present invention (5a: TNF-a, 5b: IL-6).
Figure 6 shows the survival rate of HDF (Human dermal fibroblast) cells according to the treatment by concentration of the onion extract of the present invention.
7 shows the skin dermal cell regeneration ability according to the treatment by concentration of the onion extract of the present invention.
8 shows the change in stability over time for each amount of onion extract added in the detergent composition prepared by varying the amount of onion extract added according to the present invention.
Figure 9 shows the change in pH and viscosity according to the amount of onion extract added in the detergent composition prepared by varying the amount of the onion extract of the present invention (9a: pH change, 9b: viscosity change).
10 is an evaluation of the foaming power for each experiment (Experiments 7 to 11) in which the mixing ratio of the components of the all-in-one hair & body wash cleaning composition of the present invention is changed.

The present invention provides a human body cleansing composition comprising an onion extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

‘Onion (Alliumcepa)’ is a perennial plant belonging to the family Liliaceae, and with a long cultivation history, it is used as food, spice, and medicine with a unique taste and aroma. 100g of onion contains 90g of water, about 7.2g of carbohydrate, 1.1g of protein, 10~20mg of vitamin C, calcium, phosphorus, and iron. Onion skin contains 50.4% carbohydrate, 29.24% fiber, 1.48% fat, 4.99% ash. etc. are included.

Onions are known for their antioxidant, blood lipid lowering, antihypertensive, anti-inflammatory, antibacterial, antitumor, and blood sugar lowering properties. The main physiologically active substances contained in onions include organosulfur compounds and flavonoid compounds. In particular, onions contain quercetin, an antioxidant, very high compared to other vegetables and fruits. Quercetin is a plant-derived flavonoid phenolic compound. It is not only a strong anti-oxidant, but also has anti-arteriosclerosis, antibacterial action, cholesterol lowering, anticancer, antiviral and antiallergic activity, and almost no toxicity. known not to appear.

As a suitable solvent for extracting the onion extract of the present invention, any pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used, but is not limited thereto, for example, purified water (water ), methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), alcohols having 1 to 4 carbon atoms, including butanol (butanol), acetone (acetone), ether (ether), benzene (benzene) ), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), hexane (hexane) and various solvents such as cyclohexane (cyclohexane) can be used alone or in mixture. Preferably, water is used.

As the extraction method, any one of methods such as hot water extraction, cold extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method. There is no limitation on the method for preparing the extract of the present invention, and any known method may be used.

For example, the extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying the primary extract extracted by the hot water extraction or solvent extraction method described above. In addition, the first extract is added using various chromatography methods such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, ion exchange chromatography, etc. It is also possible to obtain a purified fraction.

Therefore, in the present invention, the extract is a concept including all extracts, fractions, and purified products obtained in each step of extraction, fractionation or purification, and their dilutions, concentrates, or dried products.

In one embodiment of the present invention, the onion extract is minced by mixing onion, onion peel, and onion root in a ratio of 7:2:1, adding 8 to 10 times water to the minced mixture, hot water extraction, filtration, and freezing It can be prepared through a drying step.

The onion extract of the present invention prepared by the above method has a total polyphenol content of 932.62±24.39 mg/100g, a total flavonoid content of 566.67±41.67 mg/100g, and a quercetin content of 63.78±1.96 mg/100g. .

The onion extract of the present invention prepared by the above method has excellent antioxidant activity, anti-inflammatory activity and skin regeneration activity.

The onion extract of the present invention may be included in the composition in an amount of 0.1 to 0.5% by weight, preferably 0.5% by weight.

In the following examples of the present invention, the pH and viscosity stability of the detergent composition for each addition amount of the onion extract that imparts functionality was checked to derive the optimal amount added, and as a result, the onion extract of the present invention was added to the detergent composition by 0.75 wt% It was found that the stability and pH of the composition decreased sharply when added.

Accordingly, in the present invention, while maximizing the functional effect of the onion extract, the optimal amount added was set to 0.5% by weight in consideration of the pH change.

In one embodiment of the present invention, the human body cleansing composition of the present invention is an active ingredient in addition to onion extract, in addition to glycerin, betaine, xanthan gum, panthenol, polyquaternium-51, polylysine, caprylic glycol, ethylhexyl Glycerin, Butylene Glycol, Decyl Glucoside, Lauryl Glucoside, Coco-Glucoside, Sodium Laureth Sulfate, Sodium Cocoyl Glutamate, PEG-120 Methyl Glucose Dioleate, Apricot Seed Oil, Jojoba Seed Oil, Evening Primrose Oil, Tea Tree leaf oil, orange flower oil, sentient geranium oil, marjoram flower oil, chamomile flower oil, cypress fruit oil, lavender oil, orange peel oil and jasmine flower oil may be further included.

Specifically, the human body cleansing composition of the present invention is 0.5% by weight of onion extract, 3% by weight of glycerin, 1% by weight of betaine, 0.5% by weight of xanthan gum, 0.5% by weight of panthenol, 0.5% by weight of polyquaternium-51, polylysine 0.25% by weight, caprylic glycol 0.25% by weight, ethylhexylglycerin 0.25% by weight, butylene glycol 0.25% by weight, decylglucoside 8% by weight, laurylglucoside 8% by weight, coco-glucoside 8% by weight, sodium laur Less sulfate 5% by weight, sodium cocoyl glutamate 5% by weight, PEG-120 methylglucose dioleate 2.5% by weight, apricot seed oil 0.338% by weight, jojoba seed oil 0.279% by weight, evening primrose oil 0.2490% by weight, tea tree leaf Oil 0.1000% by weight, orange flower oil 0.0075% by weight, sentient geranium oil 0.0075% by weight, marjoram flower oil 0.0075% by weight, chamomile flower oil 0.0045% by weight, cypress oil 0.0020% by weight, lavender oil 0.0020% by weight, orange peel oil It may include 0.0020% by weight and 0.0010% by weight of jasmine flower oil.

When the human body cleansing composition of the present invention is prepared with the components and composition ratio (mixing ratio) as described above, it has a very excellent effect in cleaning power, foaming power, and formulation aptitude, and it has been confirmed through experiments that there is almost no skin irritation.

Therefore, the composition of the present invention composed of the above components and mixing ratio has excellent cleaning power and no skin irritation, so it can be usefully used as an all-in-one hair & body wash cleaning composition that can clean the whole body skin.

Hereinafter, the present invention will be described in more detail through examples. These Examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these Examples.

<Example 1>

Onion Extract Preparation

To prepare the onion extract used in this test, onion, onion peel, and root were mixed at a ratio of about 7:2:1 and cut into appropriate sizes of 1 cm or less. 10 kg of the mixture was added with 10 times constant (or purified water, 100 kg), hot water extraction was performed in boiling water of 95° C. or higher for 4 hours, and filtered using a 55 μm bag filter. After that, it was finally prepared as a freeze-dried product using a freeze-drying machine (Ilshinbiobase Co., Ltd, Gyeonggi, Korea) and then used in the experiment.

<Example 2>

Analysis of functional components of onion extract

In order to find out the functional components of the onion extract prepared in <Example 1>, the total polyphenol content, the total flavonoid content, and the quercetin content of the onion extract were analyzed in this experiment.

<2-1> Total polyphenol content

In this experiment, the total polyphenol content contained in the onion extract prepared in <Example 1> was measured. For reference, polyphenolics are natural pigments widely present in nature, mainly in free, ester, or combined form in vacuoles and cell membranes (Naczk et al., 2003). , is known to have several physiological and pharmacological effects, such as anti-diabetic, antioxidant and anti-aging (Nakatani et al., 1990).

The total phenol content was measured by modifying the color development method (Singleton and Rossi 1965) using Folin-Cioaclteu's phenol reagent. The extract was dissolved in distilled water to prepare a sample of 200 ppm, 200 μL of it was taken, and 100 μL of 50% Folin-Cioaclteu’s phenol reagent was added. After reacting them at room temperature for 30 minutes, absorbance was measured at 750 nm, and the above process was repeated three times. The measured value was presented as the amount of gallic acid (mg GAE/g DW) contained in 1 g of extract using a gallic acid standard quantification curve.

As a result, as shown in Table 1 below, the total polyphenol content of the onion extract of the present invention was confirmed to be 932.62±24.39 mg/100g (dry form).

<2-2> Total flavonoid content

The total flavonoid content was measured by modifying the color development method using diethylene glycol (Abeysinghe et al., 2007). Extracts and fractions were dissolved in distilled water to prepare a sample of 200 ppm, and 200 μL of it was taken and mixed with 2 mL of diethylene glycol. After adding 200 μL of 1N NaOH solution and reacting for 1 hour at 37° C. (water bath), absorbance was measured at 420 nm. The above process was repeated 3 times, and the measured value was presented as the amount of quercetin (mg QE/g DW) contained in 1 g of extract or fraction using the naringin standard quantitation curve.

As a result, as shown in Table 1 below, the total flavonoid content of the onion extract of the present invention was confirmed to be 566.67±41.67 mg/100g (dry form).

<2-3> Quercetin content using HPLC

In this experiment, the quercetin content in the onion extract was measured using HPLC. For reference, quercetin, one of the flavonoid components of onions, is a powerful antioxidant that prevents oxidative damage to cells and rancidity of fat. .

After taking about 5 mg of the quercetin standard, using a 5 mL diaphragm flask, use DMSO up to the mark to achieve a concentration of 1 mg/mL, and this was used as a stock solution. Working solutions were prepared using stock solutions 6.25, 12.5, It was used after dilution with DMSO to 25, 50, and 100 μg/mL. After weighing about 2 mg of the freeze-dried sample, the test solution was filled with DMSO up to the mark to a concentration of 1 mg/mL, filtered with a 0.45 μm syringe filter (Whatman, Maidstone, UK), and analyzed by HPLC. The column used for analysis was an ODS column (YMC-Pack ODS-AM column, 4.6 X 250 mm, 5 um), and gradient elution using acetinitrile (A) and 0.1% phosphoric acid (B) was used as the mobile phase. Injection volume was 10 μL, flow rate was 1.0 mL/min, and it was confirmed at UV 334 nm wavelength and then quantified.

As a result, as shown in FIG. 1 and Table 1 below, the quercetin content in the onion extract of the present invention was confirmed to be 63.78±1.96 mg/100g (dry form).

Results of analysis of the content of three index components of the onion extract of the present invention Concentration (mg/100g) dry sample Quercetin Total phenol Total flavonoids repeat 1 65.42 955.23 566.67 Repeat 2 61.61 906.78 525.00 Repeat 3 64.32 935.85 608.33 Mean±SD 63.78±1.96 932.62±24.39 566.67±41.67

<Example 3>

Analysis of Antioxidant Activity of Onion Extract

In order to investigate the antioxidant activity of the onion extract prepared in <Example 1>, the DPPH radical scavenging activity of the onion extract was analyzed in this experiment. For reference, among the antioxidant activity measurement methods, scavenging activity using DPPH radical measures the activity of an antioxidant substance that scavenging DPPH, a stable radical. DPPH is a relatively stable free radical with a dark purple color. It is used to search for antioxidants by using the reduced and discolored ones.

To 200 μL of 200 μM DPPH (1,1-diphenyl-2-picryl hydrazyl) dissolved in methanol, add 50 μL of each diluted sample to a concentration of 1000, 500, 250, 125, 62.5, and 31.25 μg/mL, After reacting for minutes, absorbance was measured at 405 nm using a microplate reader. Ascorbic acid (Ascorbic acid) was used as a positive control, and the absorbance reduction rate of the onion extract treated group and the non-treated group was expressed.

As a result, as shown in FIG. 2, it was confirmed that the antioxidant activity of the onion extract of the present invention was increased in a concentration-dependent manner from the intermediate concentration of 125 μg/mL to 1000 μg/mL.

<Example 4>

Analysis of anti-inflammatory activity of onion extract

In order to investigate the anti-inflammatory activity of the onion extract prepared in <Example 1>, in this experiment, the ability of the onion extract to inhibit NO production and inhibit inflammatory cytokines (TNF-a, IL-6) was evaluated.

<4-1> Cell culture

Raw 264.7 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/mL), and streptomycin (100 ug/mL) at 37°C, 5% CO ₂ conditions. . Cells were cultured in a 75 cm ² flask, and the culture surface was washed with a phosphate buffered saline (PBS) solution at intervals of 3 days in culture, and then the cells were detached and suspended in a DMEM medium supplemented with 10% FBS at a certain ratio. and then transferred to a 75 cm ² flask and cultured in an incubator at 37 ° C., 5% CO ₂ condition.

<4-2> Cell viability measurement

MTT assay was performed to evaluate the cytotoxicity of the onion extract prepared in <Example 1>. For reference, in the MTT assay, MTT tetrazolium, a yellow water-soluble substrate, was converted to blue-purple insoluble MTT formazan(3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyl-tetrazolium bromide by dehydrogenase action. ), the absorbance of MTT formazan is maximized at a wavelength of 570 nm, indicating that the cells measured at this wavelength are living and metabolically active cells.

Raw264.7 cells were cultured in DMEM cell culture medium containing 10% FBS at 37° C. and 5% CO ₂ condition, and then laid out in a 96-well plate to become 2×10 ⁴ cells, and then cultured for 12 hours. Cells were completely adhered, and the extracts were treated for 24 hours at different concentrations and cultured, after which 20 uL of MTT solution was added to each well and incubated for 2 hours. After removing all the medium, DMSO was added 200uL per well to dissolve formazan, and then absorbance was measured at 570nm using a microplate reader. . On the other hand, the cytotoxicity criterion was set to 80% or less of viability.

As a result, as shown in FIG. 3, it was confirmed that the onion extract of the present invention had no toxicity up to a concentration of 1000 ug/mL.

<4-3> NO production inhibitory ability evaluation

NO assay was performed to determine the NO production inhibitory ability of the onion extract prepared in <Example 1>. For reference, macrophages are cells that play a protective role in the inflammatory response. Nitric oxide (NO), which is produced by iNOS by lipopolysaccharide (LPS) present in the outer membrane of Gram-negative bacteria, is inflammatory. triggers are created. L-arginine, a substrate of NO, is converted into L-citrulline and nitrogen monoxide, which are converted into stable nitrogen dioxide, nitrite, and nitrate. Griess reagent chemically reacts with nitrite to form purple azo salt, which is consistent with the concentration of nitrogen monoxide. The degree of production can be compared.

Put 100 uL of 2×10 ⁴ cells/well cells in a 96-well plate and incubate for 12 hours in an incubator at 37° C. and 5% CO ₂ conditions to make the cells completely adhere, and induce inflammation at the same time as the extract treatment. The supernatant was separated after co-treatment for 24 hours at a concentration of 1 ug/mL of the substance LPS. 100 uL of the separated culture supernatant was treated with the same amount of Griess reagent and reacted for 10 minutes, then absorbance was measured using a microplate reader, and the concentration of NO was obtained using Sodium nitrite (NaNO ₂ ) Comparison with a standard straight line was calculated.

As a result, as shown in FIG. 4, it was confirmed that NO production was significantly increased during LPS treatment, and when the onion extract of the present invention was treated in combination, concentration-dependent NO production from 62.5 ug/mL to 1000 ug/mL It was confirmed that this was suppressed. Through this, it was found that the onion extract of the present invention has anti-inflammatory activity by effectively inhibiting NO production.

<4-4> Inhibition of inflammatory cytokines

In order to examine the inhibitory activity of inflammatory cytokines TNF-a and IL-6 of the onion extract prepared in <Example 1>, an ELISA assay was performed. For reference, macrophages are cells that play a protective role in the inflammatory response and increase the secretion of inflammatory cytokines such as TNF-a and IL-6 by LPS present in the outer membrane of Gram-negative bacteria. Therefore, in this experiment, the degree of inhibition of inflammatory cytokines was evaluated in order to confirm the anti-inflammatory activity of the onion extract.

Put 100 uL of 2×10 ⁴ cells/well cells in a 96-well plate and incubate for 12 hours in an incubator at 37° C. and 5% CO ₂ conditions to make the cells completely adhere, and induce inflammation at the same time as the extract treatment. The supernatant was separated after co-treatment for 24 hours at a concentration of 1 ug/mL of the substance LPS. The content of IL-6, an inflammatory cytokine, was measured in the isolated culture supernatant. The cytokine content was measured using an ELISA kit (eBioscience Co., San Diege, CA, USA), and the cytokine concentration was calculated from a standard curve calculated from the standard solution included in the kit.

As a result, as shown in FIG. 5, it was confirmed that the production of inflammatory cytokines TNF-a and IL-6 increased during LPS treatment, respectively, and when the onion extract of the present invention was treated in combination, the TNF-a production amount was 62.5 ug/mL to a concentration of 1000 ug/mL in concentration-dependently decreased (see 5a), and it was confirmed that the amount of IL-6 production also decreased in a concentration-dependent manner from 31.25 ug/mL to a concentration of 1000 ug/mL (see 5b). Through the above results, it was found that the onion extract of the present invention can effectively inhibit inflammatory cytokines.

<Example 5>

Analysis of skin regeneration ability of onion extract

The dermal cell regeneration ability of the onion extract prepared in <Example 1> was evaluated.

<5-1> Cell culture

HDF (Human dermal fibroblast) cells are DMEM supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/mL), and streptomycin (100 ug/mL) at 37°C and 5% CO ₂ conditions. Incubated with the medium. Cells were cultured in a 75 cm ² flask, and the culture surface was washed with a phosphate buffered saline (PBS) solution at intervals of 3 days in culture. Then, the cells were detached using 0.25% Trypsin-EDTA and 10% After being suspended in DMEM culture medium with FBS added, it was transferred to a 75 cm ² flask and cultured at 37° C., 5% CO ₂ condition in an incubator.

<5-2> Cell viability measurement

MTT assay was performed to evaluate the viability of HDF (Human dermal fibroblast) cells according to the onion extract treatment prepared in <Example 1>.

Specifically, HDF cells were cultured in DMEM cell culture medium containing 10% FBS at 37° C. and 5% CO ₂ condition, and then laid out in a 96-well plate to become 1×10 ⁴ cells, and then cultured for 12 hours. While the cells were completely attached, the extracts were treated for 24 hours at different concentrations and cultured, after which 20 uL of MTT solution was added to each well and incubated for 2 hours. After removing all the medium, DMSO was added 200uL per well to dissolve formazan, and absorbance was measured at 570nm using a microplate reader. it was On the other hand, the cytotoxicity criterion was set to 80% or less of viability.

As a result, as shown in FIG. 6 , it was confirmed that the onion extract of the present invention had no toxicity up to a concentration of 1000 ug/mL.

<5-3> Evaluation of skin dermal cell regeneration ability

In order to evaluate the skin dermal cell regeneration ability of the onion extract prepared in <Example 1>, an in vitro scratch assay was performed.

In detail, HDF cells (Human Dermal Fibroblast) were cultured in DMEM cell culture medium containing 10% FBS at 37° C. and 5% CO ₂ condition, and then in a 6-well plate (well) to 5×10 ⁵ cells per well. plate), the cells were completely adhered while incubating for 12 hours, and the center of the plate was scraped using a Scratcher (SPL). After the sample was treated for 8 hours, the reduction in the scratched area was expressed as a percentage. As a positive control, adenosine was used.

As a result, as shown in FIG. 7 , it was confirmed that the dermal cell regeneration ability was increased in the experimental group treated with the onion extract of the present invention, and the dermal cell regeneration activity was higher than that of adenosine as a positive control.

<Example 6>

Preparation of cleaning composition containing onion extract in various concentrations and determination of optimal addition ratio with excellent stability and formulation of the composition

A cleaning composition comprising the onion extract prepared in <Example 1> in various concentrations (range of 0.1% to 2.0%) was prepared, and the optimum addition ratio was determined with excellent stability and formulation of the product.

<6-1> Selection of onion extract content range

In this experiment, a cleaning composition using onion extract as a main raw material was prepared with the components and mixing ratios shown in Table 2 below.

In order to determine the optimal mixing ratio of raw materials considering the stability of raw materials, a basic base is prepared by mixing only a minimum of cosmetic raw materials, and onion extract is added by content (0.1, 0.25, 0.5, 0.75, 1.0, 2.0%) to make the product. prepared.

Cleaning composition containing the onion extract of the present invention Prize Raw material name Mixing ratio (wt%) Experiment 1 Experiment 2 Experiment 3 Experiment 4 Experiment 5 Experiment 6 A Purified water To 100 To 100 To 100 To 100 To 100 To 100 Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 glycerin 2.0 2.0 2.0 2.0 2.0 2.0 B Decyl Glucoside 10 10 10 10 10 10 Lauryl Glucoside 10 10 10 10 10 10 Coco-Glucoside 10 10 10 10 10 10 C Sodium Benzoate 0.5 0.5 0.5 0.5 0.5 0.5 D onion extract 0.1 0.25 0.5 0.75 1.0 2.0 A: water phase, B: surfactant, C: preservative, D: extract raw material

<6-2> Stability test of composition with time change according to the amount of onion extract added

As a stability test for confirming the suitability of the formulation of the onion extract prepared in <Example 1> in cosmetic formulation, the quality stability and change in physical properties of the product due to the inherent color of the main raw material were confirmed.

Over time (0, 5, 12, 19, 26, 33) while exposing the cleaning composition prepared in Example <6-1> to each temperature condition (room temperature (25°C), 4°C, 45°C) and under ultraviolet light , 40, 48 hours) of the composition over time (appearance, discoloration, discoloration, separation) was confirmed. As a control, a basic base that does not contain onion extract components was used.

- Test criteria: There should be no discoloration or odor at UV (stored in a sunny window), 25℃ (room temperature), 4℃, 45℃. Stability was checked over a period of 50 days. Indicate the stability of change with time (O: No abnormality (5 points), X: Change in appearance, discoloration, and discoloration (0 points))

As a result, as shown in FIG. 8 , when the onion extract added content of the present invention was 0.75% or more, changes in properties and discoloration due to ultraviolet rays began to appear over time, and when the content was 0.5% or less, temperature-dependent It was confirmed that no changes over time such as appearance, discoloration, and odor due to UV rays were observed. Based on the above results, the optimal amount of onion extract of the present invention was set to 0.5%.

<6-3> Confirmation of pH and viscosity stability of the composition for each content of onion extract added

The pH and viscosity stability of the cleaning composition prepared in Example <6-1> were confirmed. When measuring viscosity and pH, it was measured while standing in a thermostat maintained at 25°C.

As a result, as shown in FIG. 9 and Table 3 below, it was confirmed that the pH change indicating the stability of the raw material rapidly decreased from the onion extract added amount of 0.75% or more. On the other hand, in the case of viscosity, which is one of the important factors reflecting stability, there was no significant difference according to the amount of onion extract added.

Therefore, the optimum added content of onion extract considering the pH change was set to 0.5% or less.

pH and viscosity of the cleaning composition according to the amount of the onion extract of the present invention Raw material content control Experiment 1 Experiment 2 Experiment 3 Experiment 4 Experiment 5 Experiment 6 pH 7.2 7.1 7.0 6.8 6.1 5.6 5.3 Viscosity (cp) 13500 13420 13280 13250 13180 13090 12860

<Example 7>

Manufacturing and quality evaluation of the all-in-one hair & body wash cleaning composition of the present invention

In <Example 6>, it was confirmed that the optimal addition amount of onion extract having excellent product stability and formulation was 0.5%. prepared. On the other hand, in order to confirm the stability of the all-in-one hair & body wash cleaning composition of the present invention, a stability test was conducted on five compositions with different surfactant concentrations according to the basic formulation of the prototype.

<7-1> Preparation of all-in-one hair & body wash cleaning composition

The components and mixing ratios of the all-in-one hair & body wash cleaning composition of the present invention are shown in detail in Table 4 below. In order to minimize skin irritation during manufacturing, a nonionic surfactant, which is a naturally-derived surfactant with the lowest degree of irritation among surfactants used in the basic formulation, was selected as the main base. In the case of a nonionic surfactant, compared to a chemical surfactant, it may be functionally inferior, so an anionic surfactant was added as an auxiliary.

All-in-one hair & body wash cleansing composition with onion extract as the main ingredient Prize Raw material name Mixing ratio (wt%) Experiment 7 Experiment 8 Experiment 9 Experiment 10 Experiment 11 A Purified water (solvent) To 100 To 100 To 100 To 100 To 100 Glycerin (humectant) 5.0 3.0 4.0 3.0 5.0 Betaine (moisturizer) 1.0 1.0 1.0 1.0 1.0 Xanthan Gum (Thickener) 1.0 1.0 1.0 1.0 1.0 Panthenol (P-conditioning agent) 0.5 0.5 0.5 0.5 0.5 Polyquaternium-51
(film former, skin moisturizer) 1.0 0.5 0.5 0.3 0.5 Polylysine / Caprylic Glycol / Ethylhexyl Glycerin / Butylene Glycol
(preservatives and moisturizers) 1.0 1.0 1.0 1.0 1.0 B Decyl Glucoside
(Non-ionic surfactant) 6.0 8.0 7.0 8.0 8.0 Lauryl Glucoside
(Non-ionic surfactant) 6.0 8.0 7.0 8.0 7.0 Coco-Glucoside
(Non-ionic surfactant) 6.0 8.0 7.0 8.0 7.0 Sodium Laureth Sulfate
(anionic surfactant) 5.0 5.0 5.0 5.0 5.0 Sodium Cocoyl Glutamate
(anionic surfactant) 2.5 3.0 3.0 2.0 5.0 PEG-120 Methyl Glucose Dioleate
(Cation surfactant, thickener) 3.0 2.5 3.0 1.0 2.0 C Apricot seed oil (flavoring agent) 0.338 0.338 0.338 0.338 0.338 Jojoba seed oil (flavoring agent) 0.279 0.279 0.279 0.279 0.279 Evening primrose oil (flavoring agent) 0.2490 0.2490 0.2490 0.2490 0.2490 Tea tree leaf oil (flavoring agent) 0.1000 0.1000 0.1000 0.1000 0.1000 Orange flower oil (flavoring agent) 0.0075 0.0075 0.0075 0.0075 0.0075 Scented Geranium Oil (Fragrance) 0.0075 0.0075 0.0075 0.0075 0.0075 Marjoram Flower Oil (Fragrance) 0.0075 0.0075 0.0075 0.0075 0.0075 Chamomile flower oil (fragrance) 0.0045 0.0045 0.0045 0.0045 0.0045 Dupine fruit oil (flavoring agent) 0.0020 0.0020 0.0020 0.0020 0.0020 Lavender oil (flavoring agent) 0.0020 0.0020 0.0020 0.0020 0.0020 Orange peel oil (flavoring agent) 0.0020 0.0020 0.0020 0.0020 0.0020 Jasmine Flower Oil (Fragrance) 0.0010 0.0010 0.0010 0.0010 0.0010 D Onion Extract (Efficacy Ingredients) 0.5 0.5 0.5 0.5 0.5 A: water phase, B: surfactant, C: fragrance, D: extract raw material

<7-2> Evaluation of cleaning power of all-in-one hair & body wash cleaning composition

The cleaning power of each composition (Experiments 7 to 11) prepared in Example <7-1> was evaluated. The detergency was evaluated by 10 adult females (19-28 years old). Detergency was evaluated as a relative score on a full scale of 10, and the average score of 10 people was expressed.

As a result, as shown in Table 5 below, the evaluation result was the highest in Experiment 8 with a score of 9.2/10.

Evaluation of cleaning power of all-in-one hair & body wash cleaning composition All-in-one hair & body wash cleaning composition Experiment 7 Experiment 8 Experiment 9 Experiment 10 Experiment 11 average score 8.3 9.2 8.5 7.9 8.2

<7-3> Evaluation of foaming power of all-in-one hair & body wash cleaning composition

Each composition (Experiments 7-11) prepared in Example <7-1> was mixed with tap water to prepare a dilution of 1 wt%, and 250 mL of each dilution was taken and the most suitable for measuring bubble force using a stirrer. After stirring for 30 seconds at a suitable rotation speed of 600 rpm to generate bubbles, the height of the foam was measured, and each experiment was repeated 3 times, and the results were shown as average values.

As a result, as shown in FIG. 10 and Table 6 below, in Experiment 8, 254 mm showed the best foaming force.

Evaluation of cleaning power of all-in-one hair & body wash cleaning composition All-in-one hair & body wash cleaning composition Experiment 7 Experiment 8 Experiment 9 Experiment 10 Experiment 11 Height of the Foam
(mm) 172 254 185 210 188

<7-4> Check pH and viscosity stability of all-in-one hair & body wash cleaning composition

7 days, 14 days, 21 days from immediately after preparation for Experiments 7-11 according to the basic formulation of the prototype in order to confirm the product stability of each composition (Experiment 7-11) prepared through Example <7-1> , 35 days, 49 days and 60 days were stored, and pH and viscosity were measured, and the viscosity and pH for each time period were measured while standing in a thermostat maintained at 25°C.

As a result, as shown in Table 7 below, it was confirmed that there was no significant change in pH and viscosity over time in all the experimental groups (Experiments 7 to 11), thereby confirming that they had physical stability.

Evaluation of pH and viscosity stability of all-in-one hair & body wash cleaning composition Classification immediately after manufacturing Day 7 Day 14 Day 21 Day 35 Day 49 Day 60 Experiment 7 pH 6.1 6.13 6.14 6.09 6.11 6.21 6.18 Viscosity (cp) 6800 6880 6910 6920 6880 6915 6910 Experiment 8 pH 6.2 6.22 6.23 6.18 6.2 6.22 6.22 Viscosity (cp) 6300 6360 6350 6340 6360 6350 6350 Experiment 9 pH 6.3 6.28 6.26 6.29 6.3 6.28 6.27 Viscosity (cp) 6540 6580 6570 6520 6580 6570 6540 Experiment 10 pH 6.4 6.43 6.42 6.39 6.42 6.41 6.38 Viscosity (cp) 6240 6280 6250 6220 6280 6275 6245 Experiment 11 pH 6.16 6.19 6.15 6.19 6.21 6.17 6.16 Viscosity (cp) 6250 6280 6270 6250 6260 6247 6253

<7-5> Formulation aptitude test of all-in-one hair & body wash cleaning composition

In order to examine the aptitude of the formulation of each composition (Experiments 7 to 11) prepared in Example <7-1>, the results of use in Experiments 7 to 11 are summarized. Satisfaction was evaluated based on three criteria of conditioning. Formulation aptitude evaluation was repeated 10 times based on a three-point scale for each evaluation item (bubbles amount, hair tangle during rinsing, dry condition conditioning), and the total was expressed as a score.

As a result, as shown in Table 8 below, in Experiment 8, the total score was 80, showing the highest score.

Formulation aptitude test result of all-in-one hair & body wash cleaning composition Classification n=1 n=2 n=3 n=4 n=5 n=6 n=7 n=8 n=9 n=10 total score Experiment 7 bubble volume 2 2 3 2 2 3 2 2 2 2 74/90 points Hair tangles when rinsing 3 3 2 3 3 3 3 2 3 2 dry condition conditioning 2 2 3 2 3 2 2 3 3 3 Experiment 8 bubble volume 3 3 3 2 3 3 2 3 2 2 80/90 points Hair tangles when rinsing 3 3 2 2 3 3 3 3 3 2 dry condition conditioning 3 3 3 2 3 3 2 3 2 3 Experiment 9 bubble volume 3 2 2 2 3 2 2 3 2 2 72 points/90 points Hair tangles when rinsing 3 3 2 2 3 3 3 2 3 2 dry condition conditioning 2 2 3 2 2 2 2 3 3 2 Experiment 10 bubble volume 2 2 3 3 2 3 2 3 3 2 62 points/90 points Hair tangles when rinsing 2 3 2 One 2 2 One 2 2 One dry condition conditioning One 2 2 2 3 2 2 2 One 2 Experiment 11 bubble volume 3 2 2 2 3 2 3 2 2 2 69/90 points Hair tangles when rinsing 2 3 2 3 2 2 2 2 3 2 dry condition conditioning 2 2 3 2 2 2 2 2 3 3

<Example 8>

Skin irritation human test of the all-in-one hair & body wash cleaning composition of the present invention

Among the compositions (Experiments 7 to 11) prepared in Example <7-1>, Experiment 8, which showed the highest satisfaction in the usability test, detergency evaluation, and foaming power evaluation, was selected as the final formulation, and A skin irritation test was performed.

The skin irritation evaluation of the final product was evaluated by ELED Co., Ltd., and after approval in accordance with the standard operating guidelines of the institutional bioethics committee within ELID, the Ministry of Food and Drug Safety Regulations on Functional Cosmetics Review (No. 2019-47), Conducted in accordance with PCPC (Personal Care Products Council) Safety Evaluation regulations, Draize Dermal Irritation Scoring System and EPA (Environmental Protection Agency) Standard Evaluation Procedure Dermal Classification System, and ELED Standard Operating Guidelines (EL-P-7400) did For 30 subjects, 35 μL of hair & body wash all-in-one product diluted with 1% in distilled water was added dropwise using Van der Bend chambers (Van der Bend, Brielle, the Netherlands) and applied closed for 24 hours. After 24 hours, the test site was dried, and after 30 minutes, 24 hours, and 48 hours, the presence or absence of irritation of the test site was observed.

Research subject basic information Registered study subjects (persons) 34 Dropout study subjects (persons) 4 Final completion study subjects (persons) 30 Mean age (standard deviation) 45.63 (6.17) Gender (ill) Female (29), Male (1)

Study Subject Age Distribution age 20's 30's 40's 50's Sum number of people(%) 1 (3.33) 4 (13.33) 15 (50.00) 10 (33.33) 30 (100.00)

Criteria for skin irritation (Draize Dermal Irritation Scoring evaluation criteria) Erythema and Eschar Formation
(erythema and crust formation) Value Edema Formation
(edema formation) Value No erythema 0 No erythema 0 Very slight erythema (barely perceptible) One Very slight edema (barely perceptible) One Well-defined erythema 2 Slight edema (edges of area well-defined by definite raising) 2 Moderate-to-severe erythema 3 Moderate edema (raised approximately 1 mm) 3 Severe erythema (beet redness) to slight, eschar formation (injuries in depth) 4 Severe edema (raised more than 1 mm and extending beyond the area of exposure) 4

- Mean Irritation Index (M.I.I.) calculation formula

EPA Dermal Classification System Stimulus group (Grade) M.I.I. Negligible 0.00≤M.I.I<0.50 Slight 0.50≤M.I.I<2.00 Moderate 2.00≤M.I.I<5.00 Strong 5.00≤M.I.I<8.00

Skin irritation degree of the all-in-one hair & body wash cleaning composition of the present invention number product name number of responders degree of skin irritation Average skin irritation 30 minutes
after elapsed 24 hours
after elapsed 48 hours
after elapsed One 2 3 4 One 2 3 4 One 2 3 4 #One Onion all-in-one shower cleanser erythema 5 One - - - One - - - 4 - - - 0.10 edema 3 - - - - - - - - 3 - - -

As a result, as shown in Table 13 above, the average skin irritation degree of the all-in-one hair & body wash cleaning composition of the present invention (product name: Onion All-in-One Shower Cleanser) was 0.10, and there was no irritation according to the skin irritation criterion in Table 12. was judged as

In addition, as a result of adverse reaction evaluation by the researcher and study subject for 30 study subjects who completed the final test, this product did not show any special skin adverse reactions other than the skin reactions expected or accompanied by the patch test during the study period. didn't

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (8)

A human body cleansing composition comprising onion extract as an active ingredient. According to claim 1,
The onion extract is minced by mixing onions, onion skins, and onion roots in a ratio of 7:2:1, and hot water extraction is performed by adding 8 to 10 times water to the minced mixture, filtered, and then freeze-dried. Characterized in that, the human body cleansing composition. According to claim 1,
The onion extract has a total polyphenol content of 932.62±24.39 mg/100g, a total flavonoid content of 566.67±41.67 mg/100g, and a quercetin content of 63.78±1.96 mg/100g, a human body cleansing composition. According to claim 1,
The onion extract is characterized in that it has antioxidant activity, anti-inflammatory activity and skin regeneration activity, a human cleansing composition. According to claim 1,
The onion extract is contained in the composition in an amount of 0.1 to 0.5% by weight, the human body cleansing composition. According to claim 1,
In addition to the onion extract, the composition contains glycerin, betaine, xanthan gum, panthenol, polyquaternium-51, polylysine, caprylic glycol, ethylhexyl glycerin, butylene glycol, decyl glucoside, lauryl glucoside, coco- Glucoside, Sodium Laureth Sulfate, Sodium Cocoyl Glutamate, PEG-120 Methyl Glucose Dioleate, Apricot Seed Oil, Jojoba Seed Oil, Evening Primrose Oil, Tea Tree Leaf Oil, Orange Flower Oil, Scented Geranium Oil, Marjoram Flower Oil , Chamomile flower oil, cypress fruit oil, lavender oil, orange peel oil and jasmine flower oil, characterized in that it further comprises a human body cleansing composition. 7. The method of claim 6,
The composition comprises onion extract 0.5% by weight, glycerin 3% by weight, betaine 1% by weight, xanthan gum 0.5% by weight, panthenol 0.5% by weight, polyquaternium-51 0.5% by weight, polylysine 0.25% by weight, caprylic acid Cole 0.25% by weight, ethylhexylglycerin 0.25% by weight, butylene glycol 0.25% by weight, decylglucoside 8% by weight, lauryl glucoside 8% by weight, coco-glucoside 8% by weight, sodium laureth sulfate 5% by weight, sodium coco Il glutamate 5% by weight, PEG-120 methylglucose dioleate 2.5% by weight, apricot seed oil 0.338% by weight, jojoba seed oil 0.279% by weight, evening primrose oil 0.2490% by weight, tea tree leaf oil 0.1000% by weight, orange flower oil 0.0075% by weight, sent geranium oil 0.0075% by weight, marjoram flower oil 0.0075% by weight, chamomile flower oil 0.0045% by weight, cypress oil 0.0020% by weight, lavender oil 0.0020% by weight, orange peel oil 0.0020% by weight, and jasmine flower oil 0.0010 % by weight, the body cleansing composition. According to claim 1,
The composition is characterized in that it can be used simultaneously as a hair shampoo and a body wash, a human body cleansing composition.

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