KR20220030370A - Composition for antifatique or exercise performance comprising mixed extract of Atractylodes macrocephala and Agastache rugosa - Google Patents

Abstract

The present invention relates to a composition for alleviating fatigue or enhancing exercise performance, including a mixed extract of Atractylodes macrocephala and Agastache rugosa. According to the present invention, the composition including a mixed extract of Atractylodes macrocephala and Agastache rugosa as an active ingredient increases the expression of PGC-1 alpha and enhances exercise performance by reducing serum, intramuscular lactic acid, and lactate dehydrogenase in the serum after performing physical exercise, so that accumulated fatigue can be effectively alleviated and exercise performance can be enhanced, thereby being applied as a food composition, a pharmaceutical composition, a cosmetic composition for alleviating fatigue or enhancing exercise performance, or a pharmaceutical composition for preventing or treating diseases related to fatigue or exercise performance.

Description

A composition for improving fatigue or improving exercise performance comprising mixed extract of Atractylodes macrocephala and Agastache rugosa

The present invention relates to a composition for improving fatigue or improving exercise performance comprising a mixed extract of baekchul and baekchul hyang.

Fatigue is a state of reduced mental and physical function, and in modern society, it is experienced at a high frequency regardless of age or gender. Physical fatigue accumulated through physical activity along with mental fatigue due to the effects of stress, etc., is easily observed in people's daily life, and symptoms such as muscle pain, weakness, body aches, etc. there is. According to a survey of Korean office workers, more than half are experiencing chronic fatigue, and the accumulation of physical fatigue due to decreased physical strength along with mental factors has a significant impact on daily life, leading to deterioration of physiological functions. has been reported to be

The improvement of physical fatigue means being able to sustain faster or stronger physical movements performed in daily life or sports. Increasing the endurance of muscles used in performing physical activity, suppressing induced muscle damage, and activating caloric metabolism at the same time are ways to delay and improve physical fatigue.

On the other hand, muscular strength refers to the maximum force that a muscle or muscle tissue can exert, and exercise performance refers to the ability to quickly, strongly, long, and skillfully perform physical movements performed in daily life or sports using this strength. say When muscles are lost due to congenital physical disabilities or acquired reasons such as lying in a hospital for a long period of time, muscle strength decreases and exercise performance decreases, so it is required to enhance exercise performance. It is required to enhance exercise performance.

As a method for enhancing exercise performance, scientific training according to exercise, diet, improvement of technical equipment, and ergogenic aids are used. Among the above methods, studies related to functional supplements for improving athletic performance are being actively conducted in both the East and the West. However, most of the exercise ability improvement supplements used in the West have side effects such as caffeine and anabolic steroids, and there is also a risk of doping. Accordingly, in recent years, studies to develop functional supplements using natural products with guaranteed safety are being actively conducted, but the situation is still insufficient.

Under this background, the present inventors found that the mixed extract of baekchul and baechohyang increases the expression of PGC-1alpha, and improves exercise performance through reduction of lactic acid in serum and muscle and lactate dehydrogenase in serum after physical exercise. By confirming that fatigue can be effectively improved and exercise performance can be improved, the present invention has been completed.

One object of the present invention is to provide a food composition for improving fatigue or improving exercise performance comprising a mixed extract of baekchul and baekchul hyang.

Another object of the present invention is to provide a food composition for the prevention or improvement of fatigue or exercise performance related diseases comprising a mixed extract of baekchul and baekchul.

Another object of the present invention is to provide a pharmaceutical composition for the prevention or treatment of fatigue or exercise performance-related diseases, comprising a mixed extract of baekchul and baekchulhyang.

Another object of the present invention is to provide a pharmaceutical composition for improving exercise performance to improve fatigue comprising a mixed extract of baekchul and baekchul hyang.

Another object of the present invention is to provide a cosmetic composition for improving fatigue or enhancing exercise performance, comprising a mixed extract of baekchul and baekchulhyang.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the specific descriptions described below.

In addition, those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Also, such equivalents are intended to be encompassed by the present invention.

Hereinafter, the present invention will be described in more detail.

In the present invention, the mixed extract of baekchul and baechohyang increases the expression of PGC-1alpha, and improves exercise performance by reducing lactic acid in serum and muscle and lactate dehydrogenase in serum after physical exercise, thereby effectively improving accumulated fatigue It is based on the discovery that it can improve athletic performance.

One aspect of the present invention for achieving the above object provides a food composition for improving fatigue or improving exercise performance comprising a mixed extract of baekchul and baekchul hyang.

Another example provides a food composition for anti-fatigue or exercise performance enhancement comprising a mixed extract of baekchul and baekchohyang as an active ingredient.

Another example provides a food composition for preventing or improving fatigue or exercise performance-related diseases, including a mixed extract of baekchul and baekchulhyang.

In this specification, the term 'baekchul' is referred to as the scientific name Atractylodes macrocephala . The rhizome is used as a herbal medicine called baekchul (白朮).朮 (朮) is a character made after the shape of roots and branches and leaves. It is also known as sangae because its taste is similar to Gaesa, and also called canoe because its leaves are similar to thistles. Baekchul has the effects of dryness, dampness, and anti-inflammatory effects. It is used for the treatment of (痰飮眩暈), hydrocephalus, cold sweat, and fetal instability. am. The baekchul used as a herbal material of the extract herein may be the rhizome, leaf, flower, seed, outpost, etc. of Atractylodes macrocephala .

In this specification, the term 'baechohyang' is a scientific name Agastache rugosa, and the above- ground part is used as the herbal medicine name of Gwakhyang (藿香). Guo Hyang (藿香) has the effects of aromatic hwatak (芳香化濁), Gaewei district (開胃止嘔), and published (發表解暑), and it is effective for wet and low humidity (濕濁中阻), complete vomiting (脘嘔吐) It is used for the treatment of malaise (暑濕倦怠), dysphoric fever (胸悶不舒), cold sore throat (寒濕閉暑), abdominal pain and vomiting (腹痛吐瀉), nasal pain, mild spiciness, and slightly spicy taste. It is characterized by warmth. In the present specification, baecho-hyang used as a herbal material of the extract may be above-ground parts such as flowers, leaves, stems, roots, seeds, outposts, and the like.

The baekchul and baekchohyang can be purchased commercially, or can be used without limitation that is harvested or cultivated in nature.

In the present specification, the term 'extract' is a mixed extract containing the baekchul and baekchohyang, and the baekchul and baekchohyang mixed extract can be extracted from various organs of natural, hybrid, and variegated plants, specifically roots, above-ground parts, and stems. , can be extracted from leaves, flowers, body of fruits, skins of fruits, as well as plant tissue cultures.

In addition, the extract is a concept including all those obtained by separating a certain active ingredient from a natural product (herbal drug) with an appropriate solvent without limitation on the separation method or the type of ingredient. That is, it is a concept in a broad sense that includes both extraction of a component soluble in a solvent from a natural product using water or an organic solvent, and a product obtained by extracting only a specific component such as a specific component of a natural product, such as oil. Or the extract and/or purified product obtained in each step of purification, the diluted solution and/or concentrated solution of the extract and/or purified product, the extract, the purified product, the diluted solution, and/or the dried product of the concentrate, etc. can be used in a meaning encompassing all. there is.

The active ingredient of the composition provided herein, the baekchul and baekchohyang mixed extract, means a mixture of baekchul extract and baekchohyang extract (a mixture of herbal extracts), or may be a mixed extract extracted after mixing baekchul and baekchohyang.

The baekchul extract, baekchohyang extract, and / or the extract of the mixture is a crude extract obtained by extraction, separation and / or fractionation from nature using a method known in the art for extraction, separation and / or fractionation, a solvent (polar solvent) or a non-polar solvent) soluble extract, fraction, or a combination thereof. In one embodiment, the extract of the baekchul extract, the baekchulhyang extract, and/or the mixture may be a crude extract or a solvent-soluble extract (eg, a polar solvent-soluble extract), but is not limited thereto.

The mixed extract of baekchul and baekchohyang may be extracted using baekchul, baechohyang, and/or a mixture thereof using at least one selected from water, an organic solvent, and a combination thereof as an extraction solvent. The organic solvent may be at least one selected from linear or branched chain alcohols having 1 to 4 carbon atoms (specifically, methanol, ethanol, propanol, isopropanol, butanol, isobutanol, etc.), specifically, one type or a mixture of two or more types. there is.

In one embodiment, the extraction solvent may be water, ethanol, methanol, or a combination thereof (specifically, an aqueous ethanol solution and/or an aqueous methanol solution, etc.), specifically, 10 to 80% (v/v), 15 to 80% (v/v), 20 to 80% (v/v), 25 to 80% (v/v), 28 to 80% (v/v), 30 to 80% (v/v), 10 to 70% (v/v), 15 to 70% (v/v), 20 to 70% (v/v), 25 to 70% (v/v), 28 to 70% (v/v), 30 to 70% (v/v), 10 to 60% (v/v), 15 to 60% (v/v), 20 to 60% (v/v), 25 to 60% (v/v), 28 to 60% (v/v), 30 to 60% (v/v), 10 to 50% (v/v), 15 to 50% (v/v), 20 to 50% (v/v), 25 to 50% (v/v), 28 to 50% (v/v), 30 to 50% (v/v), 10 to 40% (v/v), 15 to 40% (v/v), 20 to 40% (v/v), 25 to 40% (v/v), 28 to 40% (v/v), or 30 to 40% (v/v) of ethanol or methanol, but not limited thereto does not

In one example, the baekchul and baekchulhyang mixed extract,

(1) (i) 10 to 80% (v/v), 15 to 80% (v/v), 20 to 80% (v/v), 25 to 80% (v) of (i) whitening (eg, rhizome) /v), 28-80% (v/v), 30-80% (v/v), 10-70% (v/v), 15-70% (v/v), 20-70% (v) /v), 25 to 70% (v/v), 28 to 70% (v/v), 30 to 70% (v/v), 10 to 60% (v/v), 15 to 60% (v) /v), 20-60% (v/v), 25-60% (v/v), 28-60% (v/v), 30-60% (v/v), 10-50% (v) /v), 15-50% (v/v), 20-50% (v/v), 25-50% (v/v), 28-50% (v/v), 30-50% (v) /v), 10-40% (v/v), 15-40% (v/v), 20-40% (v/v), 25-40% (v/v), 28-40% (v) /v), or 30 to 40% (v/v) of baekchul extract extracted with ethanol or methanol, (ii) 10 to 80% (v/v), 15 to 80% (v) of baekchulhyang (eg, above ground) /v), 20-80% (v/v), 25-80% (v/v), 28-80% (v/v), 30-80% (v/v), 10-70% (v) /v), 15 to 70% (v/v), 20 to 70% (v/v), 25 to 70% (v/v), 28 to 70% (v/v), 30 to 70% (v) /v), 10-60% (v/v), 15-60% (v/v), 20-60% (v/v), 25-60% (v/v), 28-60% (v) /v), 30-60% (v/v), 10-50% (v/v), 15-50% (v/v), 20-50% (v/v), 25-50% (v) /v), 28-50% (v/v), 30-50% (v/v), 10-40% (v/v), 15-40% (v/v), 20-40% (v) /v), 25 to 40% (v/v), 28 to 40% (v/v), or 30 to 40% (v/v) of a mixture of baechohyang extract extracted with ethanol or methanol;

(2) 10 to 80% (v/v), 15 to 80% (v/v), 20 to 80% (v/v), 25-80% (v/v), 28-80% (v/v), 30-80% (v/v), 10-70% (v/v), 15-70% (v/v), 20 to 70% (v/v), 25 to 70% (v/v), 28 to 70% (v/v), 30 to 70% (v/v), 10 to 60% (v/v), 15 to 60% (v/v), 20 to 60% (v/v), 25 to 60% (v/v), 28 to 60% (v/v), 30 to 60% (v/v), 10-50% (v/v), 15-50% (v/v), 20-50% (v/v), 25-50% (v/v), 28-50% (v/v), 30-50% (v/v), 10-40% (v/v), 15-40% (v/v), 20-40% (v/v), 25-40% (v/v), an extract of the mixture extracted with 28-40% (v/v), or 30-40% (v/v) ethanol or methanol;

or (3) a combination thereof.

The extraction is 50 to 100 ℃, 60 to 100 ℃, 70 to 100 ℃, 75 to 100 ℃, 80 to 100 ℃, 85 to 100 ℃, 50 to 95 ℃, 60 to 95 ℃, 70 to 95 ℃, 75 to 95 °C, 80 to 95 °C, 85 to 95 °C, 50 to 90 °C, 60 to 90 °C, 70 to 90 °C, 75 to 90 °C, 80 to 90 °C, 85 to 90 °C, 50 to 85 °C, 60 to It may be carried out at an extraction temperature of 85 °C, 70 to 85 °C, 75 to 85 °C, or 80 to 85 °C, but is not limited thereto.

The extraction is carried out under the above-described extraction solvent and/or extraction temperature conditions, for 10 minutes or more, 30 minutes or more, 1 hour or more, 2 hours or more, or 3 hours or more, for example, 10 minutes to 12 hours, 10 minutes to 6 hours. , 10 minutes to 5 hours, 10 minutes to 4 hours, 10 minutes to 3 hours, 30 minutes to 12 hours, 30 minutes to 6 hours, 30 minutes to 5 hours, 30 minutes to 4 hours, 30 minutes to 3 hours, 1 to 12 hours, 1 to 6 hours, 1 to 5 hours, 1 to 4 hours, 1 to 3 hours, 2 to 12 hours, 2 to 6 hours, 2 to 5 hours, 2 to 4 hours, 2 to 3 hours, 3 It may be performed for 12 hours to 12 hours, 3 to 6 hours, 3 to 5 hours, or 3 to 4 hours, but is not limited thereto.

The extraction may be performed by any commonly used extraction method, and specifically, the group consisting of hot water extraction, ultrasonic extraction, reflux extraction, cold extraction, reflux cooling extraction, solvent extraction, steam distillation, elution, compression, etc. It may be performed by one or more methods selected from, but is not limited thereto.

After obtaining the extract using the extraction solvent as described above, a liquid product can be obtained by cooling, heating and/or filtration at room temperature by a conventional method known in the art, and as an optional process, additionally evaporating and spraying the solvent It may be dried or freeze-dried. In addition, the extract extracted as described above may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

The baekchuk extract, baekchohyang extract, and / or mixed extract is silica gel column chromatography (silica gel column chromatography), thin layer chromatography (thin layer chromatography), such as high performance liquid chromatography (high performance liquid chromatography) using various chromatography Thus, it can be obtained as a further purified fraction.

The mixing ratio of baekchul extract and baekchulhyang extract in the mixed extract or the mixing ratio of baekchul and baekchulhyang in the mixture of baekchul and baechohyang is, by weight, 8:1 to 1:8, 8:1 to 1:4, 8:1 to 1 :2, 8:1 to 1:1, 8:1 to 2:1, 8:1 to 3:1, 8:1 to 4:1, 6:1 to 1:1, 6:1 to 2:1 , 6:1 to 3:1, 6:1 to 4:1, 5:1 to 1:1, 5:1 to 2:1, 5:1 to 3:1, 5:1 to 4:1, or It may be about 4:1 (above, baekchul weight or solids weight of baekchul extract: baekchulhyang weight or solids weight of baekchulhyang extract), and the content of baekchul extract or baekchulhyang extract or baekchulhyang content, by weight, is twice or more, 3 times or more, or 4 times or more (specifically, 8:1 to 2:1, 8:1 to 3:1, 8:1 to 4:1, 6:1 to 1:1, 6:1 to 2:1, 6:1 to 3:1, 6:1 to 4:1, 5:1 to 1:1, 5:1 to 2:1, 5:1 to 3:1, 5:1 to 4 It may be :1, or about 4:1 (baekchul weight or solid content weight of baekchul extract: baekchulhyang weight or solids weight of baekchulhyang extract)), and the mixing ratio of each extract constituting the mixed extract is the fatigue improvement to be achieved by the present invention Alternatively, as long as it is a ratio capable of enhancing, improving, or enhancing exercise performance, it is not limited thereto and may be included in various mixing ratios.

The solid content means the weight of the solid remaining after removing the solvent component of the extract. This is a term used to indicate that when the mixture is a mixture of baekchul extract and baechohyang extract, the mixing ratio means the ratio between the weight of the active ingredient from which the extraction solvent is removed so that the composition and/or concentration of the extract is not affected.

The mixed extract of baekchul and baechohyang included as an active ingredient in the pharmaceutical composition may be in the form of a dried product, a concentrate, or a concentrated dried product.

In addition, the extract of the present invention may optionally be added to a known ingredient or extract of a known natural product for improving fatigue or enhancing exercise performance.

As used herein, the term 'fatigue (fatigue, exhaustion, tiredness, languidness, languor, lassitude, listlessness)' may mean a state or symptom in which physical and/or mental function is reduced, specifically, physical fatigue, mental fatigue Alternatively, all muscle fatigue may be included, and if it can be included in the category of other fatigue, it may be included without limitation.

In this specification, the term 'improvement of fatigue' may be used interchangeably with 'anti-fatigue', and specifically refers to the prevention, alleviation, improvement, alleviation, suppression, elimination, and/or treatment of fatigue, and/or recovery from fatigue. can do.

In the present specification, the term 'exercise performance enhancement' refers to the duration of exercise (eg, running, walking, cycling, jumping rope, climbing, swimming, aerobics, various ball games or martial arts exercises, various muscle strengthening exercises, etc.) could mean an increase.

In the present specification, food composition is meant to include health functional food, and unless otherwise stated, food composition and health functional food may be interchangeable with each other in an equivalent meaning.

'Health functional food' refers to food manufactured (processed) using raw materials or ingredients (hereinafter, 'functional raw materials') that are easily deficient in daily meals or have useful functions for the human body. It means any food that helps prevent and/or ameliorate symptoms, and there is no particular limitation on the form of the final product. For example, the health functional food may be selected from the group consisting of various foods, beverages, food additives, and the like, but is not limited thereto.

The food composition may include acceptable food supplement additives, and may further include suitable carriers, excipients and diluents commonly used in the manufacture of health functional foods.

When the food composition is used as a food additive, the composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method.

The content of the active ingredient (a mixed extract of baekchul and baechohyang) contained in the food composition may be appropriately determined without particular limitation depending on the type of food, desired effect, purpose, etc., specifically, 0.001 to 0.001 to total food weight 99% by weight, 0.001 to 90% by weight, 0.01 to 99% by weight, 0.01 to 95% by weight, 0.01 to 90% by weight, 0.01 to 80% by weight, 0.01 to 50% by weight, 0.1 to 99% by weight, 0.1 to 95% by weight %, 0.1 to 90% by weight, 0.1 to 80% by weight, 0.1 to 70% by weight, 0.1 to 60% by weight, 0.1 to 50% by weight, 0.1 to 30% by weight, 0.1 to 10% by weight, 1 to 99% by weight, 1 to 95% by weight, 1 to 90% by weight, 1 to 80% by weight, 1 to 70% by weight, 1 to 60% by weight, 1 to 50% by weight, 1 to 30% by weight, 1 to 10% by weight, 10 to 99 wt%, 10-95 wt%, 10-90 wt%, 10-80 wt%, 10-70 wt%, 10-60 wt%, 10-50 wt%, 10-30 wt%, 25-99 wt% %, 25 to 95% by weight, 25 to 90% by weight, 25 to 80% by weight, 25 to 70% by weight, 25 to 60% by weight, 25 to 50% by weight, 25 to 30% by weight, 40 to 99% by weight, 40 to 95% by weight, 40 to 90% by weight, 40 to 80% by weight, 40 to 70% by weight, 40 to 60% by weight, 40 to 50% by weight, 50 to 99% by weight, 50 to 95% by weight, 50 to 90% by weight, 50 to 80% by weight, 60 to 99% by weight, 60 to 95% by weight, 60 to 90% by weight, or 60 to 80% by weight, but is not limited thereto.

There is no particular limitation on the type of the food composition. Examples of foods to which the food composition can be added include dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, and can be used as powders, granules, tablets, capsules, syrups or beverages. It can be manufactured, it can be manufactured without limitation in a known form, and it can be used to control biological defense rhythms, prevent diseases, It may include all foods designed to sufficiently express the body control functions related to recovery, etc. to the living body.

The food composition includes various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents or flavoring agents such as natural flavoring agents, coloring agents, pectic acid or salts thereof, alginic acid or salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, It may further contain one or more additives selected from the group consisting of stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, natural fruit juice, fruit juice beverages, and pulp for production of vegetable beverages. The proportion of these additives is generally selected from about 0.0001 to about 20 parts by weight per 100 parts by weight of the total health functional food, but is not limited thereto.

As used herein, the term 'fatigue or exercise performance-related disease' encompasses all diseases and/or symptoms that directly or indirectly cause fatigue or decreased exercise performance, and include muscle pain (fatigue), Examples of muscle diseases caused by decreased muscle function, muscle wasting or muscle degeneration, muscle wasting, decreased impulsivity, low endurance, mitochondrial abnormalities, degenerative diseases, weakness, body aches, digestive disorders, sleep deprivation or sleep disorders, chronic fatigue syndrome, etc. However, the present invention is not limited thereto.

In addition, the muscle disease may be any one or more selected from the group consisting of atony, muscular atrophy, muscular dystrophy, myasthenia gravis, cachexia and sarcopenia, However, the present invention is not limited thereto.

In the present specification, the term 'prevention' is to apply the mixed extract of baekchul and baechohyang of the present invention to a food composition to prevent in advance from a decrease in fatigue or a decrease in exercise performance, and acts on a subject who does not have specific symptoms or diseases. It is used to mean that the specific symptom or disease does not occur or includes all mechanisms and/or effects of delaying the occurrence time. In addition, 'improvement' means improving, enhancing, or strengthening the composition of the present invention to improve fatigue or improve exercise performance.

Another embodiment of the present invention provides a pharmaceutical composition for the prevention or treatment of fatigue or exercise performance-related diseases comprising a mixed extract of baekchul and baekchul.

In addition, the present invention provides a pharmaceutical composition for improving exercise performance to improve fatigue comprising a mixed extract of baekchul and baechohyang.

In addition, the present invention is anti-fatigue, including the step of administering to an individual in need of prevention and / or treatment of diseases related to anti-fatigue, exercise ability, or fatigue and / or exercise performance related to anti-fatigue, To provide a method for improving athletic performance, or preventing and/or treating fatigue and/or athletic performance-related diseases.

In the present specification, the terms 'baekchul', 'baekchohyang', 'mixed extract', 'fatigue', 'exercise performance', 'fatigue or athletic performance-related disease', 'prevention' and 'improvement' are as described above .

As used herein, 'treatment' refers to alleviation or amelioration of symptoms, reduction in the scope of a disease, delay or alleviation of disease progression, amelioration, alleviation or stabilization of a disease state or symptom, partial or complete recovery, prolongation of survival, or other beneficial effects. It is used in the meaning of including all treatment results, etc.

As used herein, 'administration' may refer to providing a pharmaceutical composition provided herein in a pharmaceutically effective amount to an individual by any suitable method.

The pharmaceutical composition may be administered to mammals including humans, dogs, cats, horses, cattle, pigs, goats, rabbits, mice, rats, etc. or cells, tissues, or cultures isolated therefrom by various routes. . The mode of administration may be any method commonly used, for example, oral administration, or parenteral administration such as intravenous administration, intramuscular administration, subcutaneous administration, intraperitoneal administration, rectal administration, local administration at the lesion site. The pharmaceutical composition is formulated into oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, transdermal preparations, suppositories, and parenteral dosage forms in the form of sterile injection solutions according to conventional methods. can be used

The pharmaceutical composition, in addition to the baekchul and baekchohyang mixed extract as an active ingredient, may be to further contain adjuvants such as pharmaceutically and/or physiologically acceptable carriers, excipients, and/or diluents. Examples of such carriers, excipients, or diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, and at least one selected from the group consisting of microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In the case of formulation, one or more diluents or excipients selected from the group consisting of commonly used fillers, extenders, binders, wetting agents, disintegrants, surfactants, and the like may be used. Solid preparations for oral administration include one or more selected from the group consisting of tablets, pills, powders, granules, capsules, syrups, powders, suspensions, etc., and these solid preparations include at least one excipient in the extract, For example, it may be prepared by mixing at least one selected from the group consisting of starch, calcium carbonate, sucrose, lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral administration include at least one selected from the group consisting of suspensions, internal solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners , may include one or more selected from the group consisting of fragrances, preservatives, and the like. Formulations for parenteral administration include at least one selected from the group consisting of sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, suppositories, transdermal preparations, and the like. At least one selected from the group consisting of propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as the non-aqueous solvent and suspending agent.

The pharmaceutical composition may be administered in a pharmaceutically effective amount. The term 'pharmaceutically effective amount' refers to an administered dose of an active ingredient capable of exerting a beneficial effect (eg, prevention, alleviation, amelioration, alleviation, suppression, elimination, and/or treatment) for symptoms and/or diseases, and is formulated Method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration interval, administration route, excretion rate, may be prescribed in various ways depending on various factors such as reaction sensitivity, the doctor or pharmacist Depending on the judgment, divided administration may be administered once or several times a day at regular time intervals.

The pharmaceutical composition may be administered as an individual therapeutic agent or may be administered in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. and may be administered single or multiple. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, and can be easily determined by those skilled in the art. In addition, the pharmaceutically effective amount may vary depending on the subject to be administered.

In one embodiment, the dosage (pharmaceutically effective amount) of the pharmaceutical composition is based on the weight of the mixed extract of baekchul and baechohyang, which are active ingredients,

1 mg/kg/day or more, 2 mg/kg/day or more, 3 mg/kg/day or more, 4 mg/kg/day or more, 5 mg/kg/day or more, 6 mg/kg/day or more, 7 mg /kg/day or more, 8 mg/kg/day or more, 9 mg/kg/day or more, or 10 mg/kg/day or more; and/or no more than 15 mg/kg/day, no more than 30 mg/kg/day, no more than 50 mg/kg/day, no more than 90 mg/kg/day, no more than 120 mg/kg/day, or no more than 150 mg/kg/day may be below.

In one embodiment, the pharmaceutical composition is 50-150 mg/kg/day, 60-140 mg/kg/day, 70-130 mg/kg/day, 80-120 mg based on the weight of the mixed extract of baekchul and baekchuhyang It may be administered at a dosage of /kg/day, 90-110 mg/kg/day, or about 100 mg/kg/day, but is not limited thereto.

As an active ingredient, the composition comprising the baekchul extract and the baekchulhyang extract may be administered to an individual by various routes. Any mode of administration can be envisaged, for example, oral, rectal or intravenous, intramuscular or subcutaneous.

As used herein, the term 'individual' refers to all animals, such as rats, mice, and livestock, including humans, that have or may develop fatigue or athletic performance-related diseases, and specifically, may be mammals including humans. , but not limited thereto.

In addition, the pharmaceutical composition may be used alone or in combination with methods using diet, physical therapy, and/or biological response modifiers to improve fatigue and/or exercise ability.

Another embodiment of the present invention provides a cosmetic composition for improving fatigue or enhancing athletic performance comprising a mixed extract of baekchul and baekchulhyang as an active ingredient.

In this specification, the terms 'baekchul', 'baechohyang', 'mixed extract', 'fatigue improvement', and 'exercise performance' are the same as described above.

In the present specification, the 'cosmetic composition' is applied to skin, hair, etc. to encompass all external compositions that generate advantageous effects, and the cosmetic composition provided herein is fatigue and/or exercise performance that causes disturbances in circadian rhythms, etc. It may mean to generate a physiologically and/or aesthetically beneficial effect on skin and/or hair by inhibiting or removing adverse effects on the skin due to deterioration, but is not limited thereto.

The cosmetic composition, in addition to the mixed extract of baekchul and baekchul, as an active ingredient, an antioxidant, a stabilizer, a solubilizer, a vitamin, a pigment, a solubilizer, a vitamin, a pigment, a flavoring agent, and/or a carrier commonly used in cosmetic compositions. can do. For example, the cosmetic composition may include one or more auxiliary ingredients selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, allantoin, and the like. This may be further included.

Since the cosmetic composition is basically applied to the skin and/or hair, it may be prepared in any formulation conventionally prepared with reference to a cosmetic composition in the art. Specifically, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. It is not limited. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray, or a powder.

When the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be included as carrier components. can

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be included as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propellants such as propane/butane, dimethyl ether, and the like.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, solubilizer, emulsifier, etc. may be included as carrier components, and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, fatty acid esters of sorbitan, and the like may be included.

In the case of a cosmetic composition suspension, liquid diluents such as water, ethanol, and propylene glycol as carrier components; suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tracanth, and the like may be included.

When the formulation of the cosmetic composition is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid as carrier components amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like may be included.

In one aspect, the composition of the present invention comprising baekchul extract and baekchohyang extract as active ingredients increases the expression of PGC-1alpha in cells, and lactic acid in serum and muscle and lactate dehydrogenase in serum after performing physical exercise It can be used as an effective drug, health functional food, etc. to relieve physical fatigue and improve exercise ability, as it improves exercise ability through reduction and has excellent anti-fatigue effect. In addition, there are advantages in that the number of essentially included drugs is small and drug interactions are small, so that the commercial properties are excellent and the risk of side effects is low.

1 is a graph showing a comparison of the effect on PGC-1 alpha gene expression according to the mixing ratio of baekchul extract and baechohyang extract in a mixed extract according to an embodiment.
Figure 2 is a graph showing the results of comparing the efficacy of the running lasting ability of the mixed extracts according to an embodiment by performing a forced running test using an experimental animal.
3 is a graph showing the results of comparing the effects of the mixed extracts according to an embodiment on the intramuscular lactic acid concentration after performing a forced running test using an experimental animal.
4 is a graph showing the results of comparing the effects of the mixed extracts according to an embodiment on the lactic acid concentration in the serum after performing a forced running test using an experimental animal.
5 is a graph showing the results of comparing the effects of the mixed extracts according to an embodiment on lactate dehydrogenase activity in serum after performing a forced running test using an experimental animal.

Hereinafter, the present invention will be described in more detail through examples. These Examples are for explaining the present invention in more detail, however, the following Examples, Comparative Examples, Experimental Examples and Preparation Examples are merely illustrative of the content of the present invention, and the scope of the present invention is limited to the following Examples and Comparative Examples , but is not limited to Experimental Examples and Preparation Examples. The Examples, Comparative Examples, Experimental Examples and Preparation Examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1. Preparation of extract

1.1. Preparation of single extract of baekchul

에서 3시간 동안 환류 추출하였다. 이를 20호 정성 거름종이(Whatman社)를 이용해 진공상태에서 여과한 후, 회전진공증발기 (Rotary vacuum evaporator) (EYELA, JAPAN)로 감압 농축한 다음 동결 건조기 (OPR-FDY-8612, OPERON, KOREA)로 동결 건조하여 백출 추출물을 제조하였다.After pulverizing the rhizome of Atractylodes macrocephala , 30% (v/v) ethanol of 10 times the dry weight of the pulverized rhizome of Baekchul (corresponding to 10 mL per 1 g of dry weight of Baekchul rhizome) was added. 85

was extracted under reflux for 3 hours. After filtration in vacuum using No. 20 qualitative filter paper (Whatman), it was concentrated under reduced pressure with a rotary vacuum evaporator (EYELA, JAPAN) and then freeze-dried (OPR-FDY-8612, OPERON, KOREA) and freeze-dried to prepare a baekchul extract.

1.2. Preparation of single extract of baechohyang

에서 90분동안 2회 반복하여 환류 추출하였다. 이를 20호 정성 거름종이(Whatman社)를 이용해 진공상태에서 여과한 후, 회전진공증발기 (Rotary vacuum evaporator) (EYELA, JAPAN)로 감압 농축한 다음 동결 건조기 (OPR-FDY-8612, OPERON, KOREA)로 동결 건조하여 배초향 추출물을 제조하였으며 분말형태로 -20℃에 보관하였다. After pulverizing the above-ground part of baechohyang ( Agastache rugosa ), 30% (v/v) ethanol of 10 times the volume (mL) of the dry weight of the herbal medicine is added, and 50

Reflux extraction was repeated twice for 90 minutes. After filtration in vacuum using No. 20 qualitative filter paper (Whatman), it was concentrated under reduced pressure with a rotary vacuum evaporator (EYELA, JAPAN) and then freeze-dried (OPR-FDY-8612, OPERON, KOREA) was freeze-dried to prepare a baechohyang extract and stored at -20°C in powder form.

1.3. Preparation of mixed extract containing baekchul extract and baekchuhyang extract (8:1)

A mixed extract was prepared by mixing the single baekchul extract prepared in Example 1.1 and the single baekchul extract prepared in Example 1.2 in a weight ratio of 8:1 (baekchul extract: baekchulhyang extract).

1.4. Preparation of a mixed extract comprising baekchul extract and baekchulhyang extract (4:1)

A mixed extract was prepared by mixing the single baekchul extract prepared in Example 1.1 and the single baekchul extract prepared in Example 1.2 in a weight ratio of 4:1 (baekchul extract: baekchulhyang extract).

Example 2. Measurement of PGC-1alpha expression level change using HeLa cell line

A large amount of energy required for exercise is supplied from mitochondria, a cell organelle, and to check whether the mixed extract of baekchul and baechohyang can activate mitochondrial biosynthesis, the related protein PGC (Peroxisome proliferator-activated receptor-gamma coactivator)-1alpha The change in expression level was measured.

HeLa cells were cultured using Dulbecco's Modified Eagle's Medium (DMEM) containing 1% penicillin/streptomycin. FuGENE reagent and PGC-1alpha plasmid (Addgene, #8887) inserted vector (Promega, #E2231) vector were mixed in HeLa cells prepared at a concentration of 6.0 X 10 ⁴ cells/well in a 24-well plate. It was aliquoted and reacted for 24 hours. Single and mixed extracts of baekchul and baechohyang were treated at a concentration of 50 μg/ml and reacted for 24 hours. After lysis of the cells using a cell lysis buffer, a luciferase substrate (luciferin) was treated and luminescence was measured. The luminescence measured at this time shows the degree of luciferase activity, and represents the expression level of PGC-1alpha.

As a result, as shown in Figure 1, the composition containing the mixed extract of baekchul and baechohyang as an active ingredient showed superior PGC-1 alpha expression efficacy than the single extract of baekchul and baechohyang, in particular, compared with Resveratrol used as a positive control. However, it was confirmed that the same or more excellent PGC-1 alpha expression effect was shown. That is, it shows that the mixed extract of baekchul and baechohyang has a significant synergistic effect on the expression of PGC-1alpha by the combination of each substance.

Example 3. Forced running experiment using mice and measurement of exercise capacity improvement efficacy in the 1st week

A forced running experiment was performed using 8-week-old ICR mice in order to measure the exercise capacity enhancing efficacy of the mixed extract of baekchul and baechohyang. Before the full-scale experiment, the light and dark were repeated at intervals of 12 hours for a week, and the temperature was maintained at 23 °C and the humidity was stabilized by 50%. After allocating 5-6 mice to each experimental group, 100 mg/kg baekchul and baekchul (4:1) mixed extract and 200 mg/kg baekchul, baekchul, baekchohyang single extract and baekchul and baechohyang (4:1) to each experimental group ) The mixed extract was orally administered once a day for a week using a zonae.

After 30 minutes of extract administration, each experimental group was subjected to a gradual increase in speed on the treadmill (12 m/min 10 min, 15 m/min 5 min, 18 m/min 5 min, 21 m/min 5 min, 24 m/min 5 min. , 27 m/min 10 minutes, 30 m/min until exhaustion) A forced running experiment was conducted.

The running duration of each experimental group was measured and shown in FIG. 2 . As a result, as shown in FIG. 2, when 100 mg/kg of the mixed extract of baekchul and baechuhyang was administered, it was confirmed that the running duration was significantly increased, and in the group administered with baekchul and baechohyang single extract 200 mg/kg, running It was confirmed that the duration did not increase. This indicates that the single extracts of baekchul and baechohyang do not have a significant athletic performance enhancing effect.

Example 4. Measurement of lactate concentration and lactate dehydrogenase activity in serum and muscle tissue after forced running experiment using mice

에서 보관하였고, 근육조직(장단지근 및 가자미근)을 채취하여 -80

에서 보관하였다.In order to confirm the optimal composition of the mixed extract of the present invention, a forced running experiment was performed by varying the ratio of baekchul and baechohyang. The experiment was performed in the same manner as the method performed in Experimental Example 2 above. After that, plasma was collected from the inferior vena cava of each mouse and 4

was stored at -80

was stored in

In order to measure the concentration of lactic acid in the muscle tissue, cold PBS solution was added to the muscle tissue of each mouse and pulverized. For more accurate analysis, the pulverized muscle tissue sample was deproteinized using a deproteinization kit (abcam). Lactic acid analysis was performed using a lactic acid analysis kit (abcam). The prepared muscle tissue pulverized sample was diluted and put in a 96-well plate, and the lactate enzyme and substrate included in the kit were added, followed by reaction at room temperature for 30 minutes. The absorbance was measured at 450 nm using a plate reader, and the amount of lactic acid was calculated using a standard curve, and the results are shown in FIG. 3 .

As a result, as shown in FIG. 3 , when administered at 200 mg/kg of baekchul and baekchul and baekchul and baekchohyang mixed extracts, the most significant inhibitory effect on lactic acid content in muscle tissue was confirmed.

In order to measure the concentration of lactic acid in the serum, plasma samples from each mouse were centrifuged at 3000 rpm for 10 minutes to collect serum. The lactic acid concentration measurement method was performed in the same manner as the lactic acid concentration measurement method in the muscle tissue, and the results are shown in FIG. 4 .

As a result, as shown in FIG. 4, when administered at 200 mg/kg of the mixed extract of baekchul and baechohyang, it was confirmed that the most significant serum lactic acid content inhibitory effect appeared.

To measure lactate dehydrogenase activity in serum, a lactate dehydrogenase assay kit (abcam) was used. After diluting the prepared serum sample into a 96-well plate and adding the lactate dehydrogenase substrate included in the kit, absorbance was measured at 450 nm at 3 minute intervals using a plate reader. Lactate dehydrogenase activity was calculated using a standard curve, and the results are shown in FIG. 5 .

As a result, as shown in FIG. 5, when administered at 200 mg/kg of the mixed extract of baekchul and baechohyang, it was confirmed that the most significant serum lactate dehydrogenase activity inhibitory effect appeared. This indicates that when the baekchul and baechohyang extracts are mixed, it has the best effect of relieving physical fatigue and enhancing exercise capacity.

Overall, as an active ingredient, the mixed extract of baekchul and baechohyang increases the expression of PGC-1alpha in cells and improves exercise ability by reducing lactate in the serum and muscle and lactate dehydrogenase in the serum after physical exercise. As it has a fatigue effect, it can be used as a drug effective for relieving physical fatigue and improving exercise capacity, as well as as a health functional food. In addition, there are advantages in that the number of essentially included drugs is small and drug interactions are small, so that the commercial properties are excellent and the risk of side effects is low.

Formulation examples of the composition according to an embodiment of the present invention will be described below, but other various formulations are also applicable, which is not intended to limit the present invention, but to describe the present invention in detail.

Formulation Example 1. Preparation of a pharmaceutical formulation

1-1. powder manufacturing

100 mg of the mixed extract of baekchul and baekchohyang prepared in any one of Examples 1.3 to 1.8, 100 mg of lactose, and 10 mg of talt were mixed and filled in an airtight bag to prepare a powder.

1-2. tablet manufacturing

100 mg of the mixed extract of baekchul and baechohyang prepared in any one of Examples 1.3 to 1.8, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate were mixed, and then tablets were prepared by tableting according to a conventional tablet manufacturing method. .

1-3. Capsule manufacturing

According to a conventional capsule preparation method, 100 mg of the mixed extract of baekchul and baechohyang prepared in any one of Examples 1.3 to 1.8, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate are mixed and filled in a gelatin capsule to capsule agent was prepared.

1-4. injection preparation

According to a conventional method for preparing an injection, 100 mg of the mixed extract of baekchul and baekchohyang prepared in any one of Examples 1.3 to 1.8 per 1 ampoule (2mL), an appropriate amount of sterile distilled water for injection, and an appropriate amount of a pH adjusting agent are mixed to prepare a conventional injection According to the method, the content of the above components was prepared per 1 ampoule (2 ml).

. 1-5. liquid manufacturing

According to a conventional liquid preparation method, 20 mg of the mixed extract prepared in any one of Examples 1.3 to 1.8, 10 g of isomerized sugar, and 5 g of mannitol were added and dissolved in purified water, and an appropriate amount of lemon flavor was added, and then the above ingredients were added mixed. Then, more purified water was added to adjust the total volume to 100 mL, filled in a brown bottle, and sterilized to prepare a solution.

Formulation Example 2. Preparation of food composition

2-1. Health functional food manufacturing

100 mg of mixed extract of baekchul and baekchohyang prepared in any one of Examples 1.3 to 1.8, appropriate amount of vitamin mixture, 70 g of vitamin A acetate, 1.0 mg of vitamin E, 0.13 mg of vitamin B1, 0.15 mg of vitamin B2, 0.5 mg of vitamin B6, and vitamin B12 0.2 g, vitamin C 10 mg, biotin 10 g, nicotinic acid amide 1.7 mg, folic acid 50 g, calcium pantothenate 0.5 mg, mineral mixture appropriate amount, ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25.3 mg, potassium phosphate 15 mg, dibasic calcium phosphate 55 mg, potassium citrate 90 mg, calcium carbonate 100 mg and magnesium chloride 24.8 mg were mixed, and then granules were prepared and health functional food was prepared according to a conventional method. At this time, the composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food ingredients are mixed in a preferred embodiment, but the mixing ratio may be arbitrarily modified.

2-2. Health functional beverage manufacturing

에서 교반 가열한 후 만들어진 용액을 여과하여 멸균된 2L 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관하여 건강음료를 제조하였다. 이때, 상기 조성비는 비교적 기호음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만 수요계층이나, 수요국가, 사용용도 등 지역적, 민족적 기호도에 따라서 그 배합비를 임의로 변형 실시하여도 무방하다.According to a conventional health drink manufacturing method, 100 mg of a mixed extract of baekchul and baechohyang prepared in any one of Examples 1.3 to 1.8, 15 g of vitamin C, 100 g of vitamin E (powder), 19.75 g of iron lactate, 3.5 g of zinc oxide, nicotinic acid After mixing 3.5 g of amide, 0.2 g of vitamin A, 0.25 g of vitamin B1, 0.3 g of vitamin B2 and an amount of water, 85 for about 1 hour

After stirring and heating in a filtration, the resulting solution was obtained in a sterilized 2L container, sealed and sterilized, and then stored in a refrigerator to prepare a health drink. At this time, although the composition ratio is composed of ingredients suitable for relatively preference beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demanding class, demanding country, and use.

Formulation Example 3. Preparation of a cosmetic composition

3-1. Manufacture of soft lotion

0.1% by weight of the mixed extract of baekchul and baekchohyang prepared in any one of Examples 1.3 to 1.8, 5.2% by weight of 1,3-butylene glycol, 1.5% by weight of oleyl alcohol, 3.2% by weight of ethanol, 3.2% by weight of polysorbate 20 , benzophenone-9 2.0% by weight, carboxyvinyl polymer 1.0% by weight, glycerin 3.5% by weight, a trace amount of fragrance, a trace amount of preservative and the remaining amount of purified water were mixed to prepare a softening lotion by a conventional method.

3-2. Milk lotion manufacturing

0.1% by weight of the mixed extract of baekchul and baechohyang prepared in any one of Examples 1.3 to 1.8, 5.1% by weight of glycerin, 4.2% by weight of propylene glycol, 3.0% by weight of tocopheryl acetate, 4.6% by weight of liquid paraffin, 1.0% by weight of triethanolamine, 3.1% by weight of squalane, 2.5% by weight of macadamia nut oil, 1.6% by weight of polysorbate 60, 1.6% by weight of sorbitan sesquirolate, 0.6% by weight of propylparaben, 1.5% by weight of carboxyl vinyl polymer, trace amount of fragrance, trace amount of preservative , and the remaining amount of purified water was mixed to prepare a milk lotion in a conventional manner.

3-3. Nutrient cream manufacturing

0.5% by weight of the mixed extract of baekchul and baekchohyang prepared in any one of Examples 1.3 to 1.8, 4.0% by weight of glycerin, 3.5% by weight of petrolatum, 2.1% by weight of triethanolamine, 5.3% by weight of liquid paraffin, 3.0% by weight of squalane, 2.6% by weight of beeswax %, tocopheryl acetate 5.4% by weight, polysorbate 60 3.2% by weight, carboxyl vinyl polymer 1.0% by weight, sorbitan sesquioleate 3.1% by weight, a small amount of fragrance, a small amount of preservative and the remaining amount of purified water by mixing Nutrient cream was prepared by this method.

3-4. massage cream manufacturing

0.5% by weight of the mixed extract of baekchul and baekchohyang prepared in any one of Examples 1.3 to 1.8, 4.0% by weight of glycerin, 3.5% by weight of petrolatum, 0.5% by weight of triethanolamine, 24.0% by weight of liquid paraffin, 3.0% by weight of squalane, 2.1% by weight of beeswax %, tocopheryl acetate 0.1% by weight, polysorbate 60 2.4% by weight, carboxyl vinyl polymer 1.0% by weight, sorbitan sesquioleate 2.3% by weight, a trace amount of fragrance, a trace amount of preservative and the remaining amount of purified water by mixing A massage cream was prepared by this method.

3-5. Manufacture of body cleanser for cleaning

Mix 1 g of the mixed extract of baekchul and baechohyang prepared in any one of Examples 1.3 to 1.8, 18 g of anionic surfactant, 5 g of nonionic surfactant, 7 g of glycerin, 3 g of sodium chloride, 1.5 g of natural olive liquid soap, 1 g of fragrance and 100 g of water Thus, a body cleanser for washing was prepared by a conventional method.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims and their equivalents.

Claims (12)

A food composition for improving fatigue or improving exercise performance, comprising a mixed extract of baekchul and baekchul baekchohyang.
The food composition according to claim 1, wherein the fatigue includes physical fatigue, mental fatigue or muscle fatigue.
According to claim 1,
The extract is water, a linear or branched chain alcohol having 1 to 6 carbon atoms, an organic solvent, and a solvent selected from the group consisting of mixed solvents thereof, the food composition.
According to claim 1,
The baekchul and baekchohyang will be mixed in a weight ratio of 8:1 to 1:8, a food composition.
According to claim 1,
The mixture is contained in an amount of 0.001 to 99% by weight based on the total weight of the composition, the food composition.
According to claim 1,
The food composition will be prepared as a powder, granules, tablets, capsules, syrups or beverages, the food composition.
A food composition for the prevention or improvement of fatigue or athletic performance-related diseases, comprising a mixed extract of baekchul and baekchul-hyang.
8. The method of claim 7,
The fatigue or exercise performance-related diseases include muscle pain, decreased muscle function, muscle disease due to muscle wasting or muscle degeneration, muscle wasting, hypotonia, hypostamina, mitochondrial abnormality disease, degenerative disease, weakness, body aches, digestive disorders, sleep deprivation, or Sleep disorder or chronic fatigue syndrome, the food composition.
9. The method of claim 8,
The muscle disease is dystonia (atony), muscular atrophy (muscular atrophy), muscular dystrophy (muscular dystrophy), myasthenia gravis, cachexia (cachexia) and sarcopenia (sarcopenia), any one or more selected from the group consisting of, a pharmaceutical composition.
A pharmaceutical composition for the prevention or treatment of fatigue or athletic performance-related diseases, comprising a mixed extract of baekchul and baekchul and baekchohyang.
A pharmaceutical composition for improving exercise performance to recover from fatigue comprising a mixed extract of baekchul and baekchul and baekchohyang.
A cosmetic composition for recovering from fatigue or improving athletic performance comprising a mixed extract of baekchul and baekchul.

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