JP5403569B2 - Strength enhancer - Google Patents

Description

  The present invention relates to a physical strength enhancer comprising either simnole and / or simnol ester, cholic acid, cod liver oil, cholic acid or simnol and / or simnol ester and cod liver oil.

Under normal circumstances, when glucose in blood is used for energy production, it becomes pyruvic acid by an aerobic metabolic pathway, and ATP is generated. However, since sufficient oxygen is not supplied during intense exercise, ATP and lactic acid are produced by an anaerobic metabolic pathway. The amount of ATP produced in the anaerobic metabolic pathway is less than that in the aerobic metabolic pathway, and the lactic acid produced therein is known to lead to fatigue because it reduces the motor function of the muscle. From this, it can be said that the aerobic metabolic pathway is more efficient when exercising.
In general, carbohydrates are the main energy used in exercise with a significant increase in heart rate, but in the case of long-term continuous exercise, energy metabolism cannot be caught up with carbohydrates alone, and lipids are used. Since lipid metabolism has better energy production efficiency than sugar metabolism, it is known that this leads to an increase in endurance, that is, enhancement of physical strength.

  As a result of various studies to solve such problems and improve energy production efficiency by lipid metabolism, thereby increasing endurance, that is, enhancing physical strength, the inventors of the present application have found that Simnole and / or Sim It has been found that there is a physical strength-enhancing effect by using norester, cholic acid, cod liver oil, cholic acid or simnol and / or simanol ester and cod liver oil, and has been developed as a new physical strength enhancer.

The present invention relates to a physical strength enhancer comprising 1 mg of Simanol sulfate ester Na, 125 mg of soybean isoflavone glycoside , 815 mg of cod liver oil, 5 mg of tocopherol acetate, 200 mg of ginseng extract and 54 mg of beeswax .

  The present invention relates to a physical strength enhancer comprising simnole and / or simnol ester, cholic acid, cod liver oil, cholic acid or simnol and / or simnol ester and cod liver oil. Can be achieved.

The invention of the present application may include herbal medicines that have a corrective effect, carrots, pearls, taisidins, ogis, sun yak, taisou, licorice, koi, seisei, jukujio, kashi, touki, biakusaku, kukosi, longan nikki, jinjin. More preferred are jujujio, touki, peony, kokushi, and ryuganiku, and carrots, jinjin, ogi, sanyaku, taisou, and licorice are particularly preferred.
Physiological functions are activated by these herbal medicines, and metabolism is flourishing.

The invention of the present application preferably includes a herbicidal medicinal product, peanut, crispula, chorei, takusha, inchinkou, mokuboui, bowie, casseki, tokanin, mokutsu, shazenshi, sekishozu, hanpenren, keishi (keihi), Inchinkou, Bowie, Kaseki, and Sekisho are more preferred, and peanuts, bukuryo, chorei, takusha, mokutsu, and sensation are particularly preferred.
Excess water in the body can be drained by the water-utilizing action of sand jellyfish.

  The invention of the present application preferably includes at least one of Auren, Oat, Maou, Forsythia, Keigai, Green tea, Oolong tea, Black tea, Coffee, Kidachi aloe, Tengusa, Kanten, Pepper, Ginger, Pepper. Tengusa is more preferable, and auren, autaku, mao, green tea, oolong tea, black tea, and kidachi aloe are particularly preferable.

  The invention of this application preferably contains isoflavones and isoflavone glycosides, and the isoflavones and isoflavone glycosides are particularly preferably soybean isoflavones and soybean isoflavone glycosides contained in soybeans.

The active ingredients for this purpose contained in soybeans are several isoflavone glycosides such as soybean in (Gaidin), glycitin (Glycitin), genistin (Gaistin) and the like, and their aglycones (Daizein), glycitein There are several isoflavones such as (Glycitein) and Genistein.
Soybean is a raw material for producing soybean oil, but the demand for soybean oil is large, and soybean meal, a by-product, is produced in large quantities at the same time. A part of soybean meal is used as a raw material for producing soybean protein as a raw material for food, but it is mainly used as fertilizer and feed, and its price is extremely low. Soybean isoflavone and soybean isoflavone glycosides can be produced with high purity and low cost from soybean meal that is close to industrial waste.

The crude drug of the invention of this application may be used as it is, or an extracted one may be used.
In addition, the dosage form of the physical strength enhancer of the present invention is not particularly limited, but it is administered as a tablet, capsule, powder, solid, liquid or the like.
Moreover, when administering, it is although it does not specifically limit, It is preferable to administer twice a day. It can also be used as a cosmetic.

The daily dose of cholic acid is preferably 1 to 1000 mg, more preferably 2 to 300 mg, and particularly preferably 10 to 100 mg.
The daily dose of simnole and / or simnol ester is preferably 0.1 to 100 mg, more preferably 0.1 to 50 mg, and particularly preferably 0.3 to 10 mg.

  The daily dose of cod liver oil is preferably 0.1 to 5000 mg, more preferably 1 to 3000 mg, and particularly preferably 2 to 1500 mg.

  The daily amount of isoflavone and isoflavone glycoside is preferably 1 to 500 mg, more preferably 5 to 300 mg, and particularly preferably 10 to 200 mg.

The daily amount of herbal medicines such as carrots, sandals, and aureans is preferably 1 to 20 g and particularly preferably 1.5 to 5 g.
The amount of the extract decocted with herbal medicine is preferably 100 to 600 mg, particularly preferably 200 to 400 mg.
Hereinafter, specific examples will be described.

Powder cod liver oil 100mg
Soybean isoflavone glycoside 125mg
Ginseng extract 300mg
White birch extract 300mg
Yellow Lotus Extract 300mg
Lactose 2460mg
Corn starch 400mg
Light anhydrous silicic acid 5mg
Magnesium stearate 10mg
Total 4000mg
(1g 1g, 2 packets, 2 times a day)
Similarly, powder was prepared using soy isoflavone instead of soy isoflavone glycoside.

Granule cod liver oil 100mg
Soybean isoflavone glycoside 125mg
Ginseng extract 300mg
White birch extract 300mg
Yellow Lotus Extract 300mg
Lactose 1800mg
Corn starch 800mg
Crystalline cellulose 260mg
Light anhydrous silicic acid 5mg
Magnesium stearate 10mg
Total 4000mg
(1g 1g, 2 packets, 2 times a day)
Similarly, granules were produced using soy isoflavones instead of soy isoflavone glycosides.

Spherical granule cod liver oil 100mg
Soybean isoflavone glycoside 125mg
Ginseng extract 300mg
White birch extract 300mg
Yellow Lotus Extract 300mg
Lactose 500mg
Corn starch 1500mg
Ume powder 475mg
Crystalline cellulose 400mg
Total 4000mg
(1g 1g, 2 packets, 2 times a day)
Similarly, spherical granules were produced using soy isoflavone instead of soy isoflavone glycoside.

Tablet cod liver oil 200mg
Soybean isoflavone glycoside 125mg
Ginseng extract 300mg
White birch extract 300mg
Yellow Lotus Extract 300mg
Lactose 3100mg
Carboxymethylcellulose calcium 320mg
Hydroxypropylcellulose 75mg
Crystalline cellulose 840mg
Carplex 30mg
Magnesium stearate 10mg
Total 5600mg
(1 tablet 560mg, 1 tablet 5 times, 2 times a day)
Similarly, tablets were produced using soy isoflavones instead of soy isoflavone glycosides.

Hard capsule cod liver oil 65mg
Soybean isoflavone glycoside 125mg
Ginseng extract 200mg
Birch extract 200mg
Yellow Lotus Extract 200mg
Corn starch 1000mg
Magnesium stearate 10mg
Total 1800mg
(1 capsule 300 mg, 3 capsules, 2 times a day)
Similarly, hard capsules were produced using soy isoflavones instead of soy isoflavone glycosides.

Soft capsule cod liver oil 1000mg
Soybean isoflavone glycoside 125mg
Ginseng extract 200mg
Birch extract 200mg
Yellow Lotus Extract 200mg
Beeswax 75mg
Total 1800mg
(1 capsule 300mg, 3 tablets, 2 times a day)
Similarly, soft capsules were produced using acetyl soybean isoflavone and soybean isoflavone instead of soybean isoflavone glycoside.

Drink agent cod liver oil 100mg
Soybean isoflavone glycoside 125mg
Ginseng extract 300mg
White birch extract 300mg
Yellow Lotus Extract 300mg
Royal Jelly 150mg
Riboflavin sodium phosphate 10mg
Ethanol 1.2ml
Paraoxybenzoic acid 4mg
Purified water appropriate amount total 50ml
(50ml per bottle, once a day, once a day)
Similarly, a drink was prepared using soy isoflavone instead of soy isoflavone glycoside.

Injection cod liver oil 20mg
JP sodium sulfite 4mg
JP Ammonia Water Appropriate amount JP JP Saline Solution Appropriate amount total 5ml
(1 vial 5 ml, 1 vial once, 1 day)

Lotion preparation cod liver oil 3g
Soy isoflavone glycoside 1.25g
Ginseng extract 3g
3g white birch extract
Yellow Lotus Extract 3g
99.5% ethanol 300 ml
Purified water appropriate amount total 1000ml
(10ml once, twice a day)
Similarly, a lotion preparation was produced using soy isoflavone instead of soy isoflavone glycoside.

Ointment cod liver oil 1.5g
Soy isoflavone glycoside 1.25g
Ginseng extract 3g
3g white birch extract
Yellow Lotus Extract 3g
99.5% ethanol 20 ml
Total amount of hydrophilic ointment 500g
(10g once, twice a day)
Similarly, an ointment was prepared using soy isoflavone instead of soy isoflavone glycoside.

Suppository cod liver oil 100mg
Soy isoflavone glycoside 625mg
Ginseng extract 1000mg
White birch extract 1000mg
Yellow Lotus Extract 1000mg
Cocoa butter or an appropriate base 10 or more suppositories.
(One agent at a time, twice a day)
Similarly, suppositories were prepared using soy isoflavones instead of soy isoflavone glycosides.

Powder simnol sulfate Na 1mg
Soybean isoflavone glycoside 125mg
Lactose 800mg
Corn starch 1064mg
Magnesium stearate 10mg
Total 2000mg
(1g 1g, 1 packet 1 time, 2 times a day)
Similarly, powder was prepared using soy isoflavone instead of soy isoflavone glycoside.

Granules cholic acid 60mg
Soybean isoflavone glycoside 125mg
Lactose 2700mg
Corn starch 800mg
Crystalline cellulose 300mg
Light anhydrous silicic acid 5mg
Magnesium stearate 10mg
Total 4000mg
(1g 1g, 2 packets, 2 times a day)
Similarly, granules were produced using soy isoflavones instead of soy isoflavone glycosides.

Tablet Simnol sulfate Na 1mg
Soybean isoflavone glycoside 125mg
Lactose 140mg
Crystalline cellulose 845mg
Magnesium stearate 6mg
Talc 3mg
Total 1120mg
(1 tablet 280mg, 2 tablets, 2 times a day)
Similarly, tablets were produced using soy isoflavones instead of soy isoflavone glycosides.

Soft capsule Simanol sulfate Na 1mg
Soybean isoflavone glycoside 125mg
Cod liver oil 815mg
Tocopherol acetate 5mg
Carrot extract 200mg
Beeswax 54mg
Total 1200mg
(1 capsule 300 mg, 2 capsules, 2 times a day)
Similarly, soft capsules were produced using soy isoflavones instead of soy isoflavone glycosides.

Hard capsule cholic acid 60mg
Soybean isoflavone glycoside 125mg
Corn starch 1005mg
Magnesium stearate 10mg
Total 1200mg
(1 capsule 300 mg, 2 capsules, 2 times a day)
Similarly, hard capsules were produced using soy isoflavones instead of soy isoflavone glycosides.

Soft capsule cholic acid 60mg
Soybean isoflavone glycoside 125mg
Beeswax 55mg
Edible oil 960mg
Total 1200mg
(1 capsule 300 mg, 2 capsules, 2 times a day)
Similarly, soft capsules were produced using soy isoflavones instead of soy isoflavone glycosides.

Soft capsule cholic acid 60mg
Soy isoflavone 75mg
Cod liver oil 805mg
Acetate floor ferrol 5mg
Carrot extract 200mg
Beeswax 55mg
Total 1200mg
(1 capsule 300 mg, 2 capsules, 2 times a day)
Similarly, soft capsules were produced using soy isoflavone glycosides instead of soy isoflavones.

Soft capsule Simanol sulfate Na 1mg
Soybean isoflavone glycoside 125mg
Cod liver oil 814mg
Acetate floor ferrol 5mg
Carrot extract 200mg
Beeswax 55mg
Total 1200mg
(1 capsule 300 mg, 2 capsules, 2 times a day)
Similarly, soft capsules were produced using soy isoflavones instead of soy isoflavone glycosides.

Pure injection cholic acid 20mg
Pure soybean isoflavone glycoside 20mg
Glucose 1000mg
PH adjuster Sodium carbonate Add an appropriate amount of distilled water for injection to make a total volume of 5 ml.
(1 vial 5 ml, 1 vial once, 1 day)
Similarly, an injection was prepared using soy isoflavone instead of soy isoflavone glycoside.

Drink agent Simnol sulfate Na 1mg
Soybean isoflavone glycoside 125mg
Ginseng extract 10mg
Giant extract 10mg
Royal Jelly 100mg
Thiamine nitrate 10mg
Riboflavin sodium phosphate 5mg
Pyridoxine hydrochloride 10mg
Anhydrous caffeine 50mg
Ethanol 1.2ml
Paraoxybenzoic acid 4mg
Purified water appropriate amount total 50ml
(50ml per bottle, once a day, once a day)
Similarly, a drink was prepared using soy isoflavone instead of soy isoflavone glycoside.

Reference Example 6-week-old ddY male mice were purchased and preliminarily raised for 1 week. During pre-breeding, pre-swimming (swimming for 10 minutes) was performed twice 6 days before and 3 days before the start of the experiment, and mice with significantly poor swimming ability were excluded from the experiment. After completion of the preliminary breeding, the body weight was measured and divided into 10 groups per group according to the body weight, and administration of the test sample was started.
Limit swimming was performed 1 week, 2 weeks, 3 weeks, and 4 weeks after administration of the test sample, and the swimming time was measured. Immediately after the end of the measurement of the limit swimming time at the 4th week, the head was decapitated and blood was collected. During this time, body weight and food intake were measured on the day before the swimming day. In addition, water was ingested freely, food was fasted only one night on the day before the swimming day, and other food was ingested freely.
The blood obtained at the time of decapitation was centrifuged at 7,600 × g and 4 ° C. for 15 minutes, and the blood glucose level and the lactic acid level were measured from the obtained serum using kits.
In the experimental group, one group was given a normal diet as a Control group, and the other groups were given the test sample mixed with food.
In Example 15, experiments were conducted at 100 mg / kg (5 times human dose), 200 mg / kg (10 human dose) and 400 mg / kg (20 human dose).
The high dose of each component is equivalent to 20 times the human dose. That is, in Example 15, 10 mg / kg, Simnol 0.5 mg / kg, soybean isoflavone 40 mg / kg and cod liver oil 400 mg / kg correspond to 20 times the amount of human.
Olive oil was used as a comparison with cod liver oil.
A Kyodai Matsumoto style momentum measuring water layer (FIG. 1) was used for forced swimming. Water was drawn from the bottom of the water tank by a flowing water pump (HANDY PUMP C-P60J, Hitachi, Ltd.), and a flow meter (FMK3831-1, Tokyo Flowmeter Laboratory) was connected, and water was circulated 37 cm from the bottom of the water tank. The water outlet used was a vinyl chloride pipe with holes at equal intervals, and the water flow rate was 8 L / min. A thermostat was attached to the lower part of the jet outlet and the water temperature was kept at 25 ± 2 ° C.
Measurement of the limit swimming time When the mouse enters the water and keeps swimming, it is washed away to the rear of the tank due to fatigue. There is a slanted plate at the rear of the aquarium, which creates a water flow to the bottom of the aquarium, and the mouse is drawn into the water by the water flow. The end point of swimming was defined as the point at which the water flow could not be resisted and it was completely submerged and did not rise to the surface for 7 seconds. After swimming, the mouse sucked water with a towel and returned it to the cage.
In this experiment, after 10 minutes of pre-swimming, swimming was performed again after a 10-minute break, and the time until the end of the swimming was defined as the limit swimming time.
Measurement of blood glucose level Glucose concentration in serum was measured using Glucose CII-Test Wako (Wako Pure Chemical Industries, Ltd.).
Measurement of Serum Lactic Acid Level Using F-kit L-lactic acid (JK International), the lactic acid concentration in serum was measured.
The swimming time in each group was calculated with the swimming time of the Control group as 100%.
Experimental values were expressed as mean ± standard deviation. The Student t test was used for the statistical significance test.
The results are as follows.
Limit swimming time First week of test sample administration FIG. 2 shows the average swimming time of the first week of test sample administration.
In Example 15, there was no significant difference in swimming time compared to the Control group.
Moreover, in the cholic acid, simnol and cod liver oil administration groups, although there was no significant difference from the Control group, a tendency to extend swimming time was observed.
In the invention of this application, the effect of single administration was not examined, but it seems that the result of single administration can be inferred from the results of the first week.
Second week of test sample administration FIG. 3 shows the average swimming time at the second week of test sample administration.
In the Example 15 administration group, although a significant difference was not observed as compared with the Control group, a tendency to extend swimming time was observed.
In the 10 mg / kg administration group, the cholic acid, simanol administration group, and the cod liver oil administration group of Example 15, a significant difference from the Control group was not observed, but a tendency to increase swimming time was observed.
4th week of test sample administration FIG. 4 shows the average swimming time at the 4th week of test sample administration.
In the administration group of Example 15, the swimming time was extended by 2-3 times compared to Control County at any dosage.
In the cholic acid, simnole 0.1 mg / kg and 0.5 mg / kg administration groups, and the cod liver oil 400 mg / kg administration group, the swimming time was significantly prolonged as compared with the Control group. In particular, in the Simnol administration group, the swimming time was extended at least twice as much as that in the Control group at both doses.
In the third week, the same tendency as in the fourth week was observed.
Change with time of swimming time FIG. 5 shows the swimming time from the first week to the fourth week of the 200 mg / kg administration group of Example 15 in which the swimming time was most extended in the fourth week of administration.
The swimming time of the 200 mg / kg administration group of Example 15 almost reached a plateau at the third week of administration, and was extended about 3 times as compared with the Control group at the fourth week of administration.
FIG. 6 shows blood glucose levels at 4 weeks of mice in each group.
The blood glucose level of the Control group was 32 ± 18 mg / dL.
In the 100 mg / kg and 400 mg / kg administration groups of Example 15, the blood glucose level significantly increased as compared to the Control group.
The blood glucose level significantly increased in the 0.5 mg / kg administration group, the 10 mg / kg administration group, the Simnole 0.1 mg / kg administration group, and the cod liver oil 400 mg / kg administration group of Example 15 as compared with the Control group.
The blood sugar level of olive oil used as a control was also significantly high.
Serum lactic acid level FIG. 7 shows the serum lactic acid level of each group of mice.
The lactic acid value of the Control group was 3.5 ± 0.9 mmol / L.
In the administration group of Example 15, the lactic acid value tended to be lower than that in the Control group, and particularly in the 200 mg / kg administration group of Example 15, the lactic acid value was significantly lower.
In the component of Example 15, in the cod liver oil 400 mg / kg administration group, the lactic acid value was significantly lower than that in the Control group. On the other hand, in the simnol and soy isoflavone administration group, the lactic acid level was somewhat higher than that in the Control group.
The components of Example 15 include Simnole, soybean isoflavone, cod liver oil, and other components at the same concentration, but were examined by measuring the swimming time by forced swimming. In addition, the swimming time of each of these constituent components, Simnol, soybean isoflavone, and cod liver oil, was also examined.
About swimming time As a result of administering Example 15 for 4 weeks, swimming time was prolonged significantly. This extension is presumed to be due to Simnole.
About blood glucose level As a result of administering Example 15 for 4 weeks, the blood glucose level after swimming showed the high value compared with the Control group. Thus, the phenomenon in which the blood glucose level did not decrease even after swimming is presumed to be due to Simnole and cod liver oil.
About Lactic Acid Level As a result of administering Example 15 for 4 weeks, the lactic acid level did not increase despite swimming. This phenomenon is presumed to be caused by cod liver oil.
When Example 15 was administered for 4 weeks, a significant prolongation of swimming time was observed. Generally, when intense exercise such as swimming is performed, the blood glucose level decreases and the lactic acid level increases, but Example 15 suppressed the decrease in the blood glucose level and the increase in the lactic acid level. These results suggest that energy consumption in mice given Example 15 is centered on aerobic metabolism using glucose and metabolism using lipids.
As components that contribute to this energy consumption, simnole and / or simnol ester and cod liver oil appear to be important.
The role of cod liver oil was examined this time, but the action of cod liver oil was different from that of olive oil. In the action of Example 15, cod liver oil plays a certain role, but this role does not merely provide an energy source, but it is considered that an action peculiar to cod liver oil is expressed.

  The object of the present invention is to provide a physical strength enhancer comprising either simnole and / or simnol ester, cholic acid, cod liver oil, cholic acid or simnol and / or simnol ester and cod liver oil.

Kyoto University Matsumoto style momentum measurement flowing water layer Mean swimming time of mice in the first week (mean ± SD, n = 1 Mean swimming time of mice in the second week (mean ± SD, n = 1 Mean swimming time of mice in the 4th week (mean ± SD, n = 10, * p <0.05 vs Control, **: p <0.01 vs Control) Swimming time from the first week to the fourth week of the 200 mg / kg administration group of Example 15 Blood glucose level (mean ± SD, n = 10, *: p <0.05 vs Control, **: p <0.01 vs Control, ***: p <0.001 vs Control) Serum lactic acid level (mean ± SD, n = 10, *: p <0.05 vs Control, **: p <0.01 vs Control, ***: p <0.001 vs Control) Energy production pathway Differences in blood glucose and lactate levels due to differences in glucose utilization and lipid utilization

Explanation of symbols

1 Kyoto University Matsumoto-type momentum measurement flowing water layer 2 Flow meter 3 Valve 4 Pump

Claims (1)

A physical strength enhancer comprising 1 mg of Simanol sulfate Na, 125 mg of soybean isoflavone glycoside, 815 mg of cod liver oil, 5 mg of tocopherol acetate, 200 mg of carrot extract and 54 mg of beeswax .

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