KR102449530B1 - Composition for anti-obesity containing extract of Cinnamomum cassia Blume, Atractylodes macrocephala Koidzumi, Pueraria lobata (Willd.) Ohwi, Paeonia lactiflora Pallas and Ephedra sinica Stapf. - Google Patents

Abstract

The present invention relates to a composition containing a complex extract of broiler chicken, baekchul, galgeun, peony and ephedra as active ingredients, which suppresses obesity by promoting body energy consumption. It was confirmed that the complex extract according to the present invention has an excellent effect in promoting energy metabolism by increasing the expression of PGC1-α in mitochondria in skeletal muscle and promoting phosphorylation of AMPK. It can be utilized as pharmaceuticals and health functional foods useful for the treatment, prevention, or improvement of various metabolic diseases.

Description

{Composition for anti-obesity containing extract of Cinnamomum cassia Blume, Atractylodes macrocephala Koidzumi, Pueraria lobata (Willd.) Ohwi, Paeonia lactiflora Pallas and Ephedra sinica Stepf.}

The present invention relates to a composition containing a complex extract of broiler chicken, baekchul, galgeun, peony and ephedra as active ingredients, which has anti-obesity effect through promoting body energy consumption.

Obesity treatment can be largely divided into two methods: suppressing energy intake or increasing energy expenditure. However, appetite suppressants to suppress energy absorption may act on the central nervous system and cause psychotropic side effects. In addition, there is a problem in that the basal metabolic rate is lowered according to the body homeostasis regulation mechanism during long-term dietary restriction through taking an appetite suppressant, so that the weight loss effect is gradually reduced. Therefore, for a more efficient and stable treatment of obesity, it is necessary to develop an anti-obesity drug through increased energy consumption.

Skeletal muscle is a tissue rich in mitochondria and is an important target organ for increasing energy consumption. The most important function of mitochondria is to play a role in energy metabolism in the body and is involved in the oxidation of sugars and fatty acids. Therefore, the decrease in mitochondrial function in skeletal muscle affects chronic metabolic diseases such as obesity and type 2 diabetes mellitus or age-related diseases.

Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) is known to regulate intracellular energy metabolism in brown fat, liver and muscle tissue (Finck BN) and Kelly DP, 2006). In skeletal muscle, PGC-1α is a key factor in cellular energy metabolism, regulating fatty acid oxidation and increasing mitochondrial biosynthesis.

Adenosine monophosphate-activated protein kinase (AMPK) is an enzyme involved in maintaining intracellular energy homeostasis. It promotes the oxidation of fatty acids and glucose uptake in skeletal muscle. directly increase expression.

On the other hand, broiler chicken ( Cinnamomum cassia Blume) is a medicinal plant used as a treatment for diabetes and obesity in oriental medicine. action has been reported.

Baekchul ( Atractylodes ) The root of macrocephala Koidzumi) is used for the treatment of gastrointestinal diseases, abdominal pain, and obesity. It was also confirmed that the baekchul extract exhibits anti-inflammatory, anti-ulcer, and anti-tumor effects (C.-Q. Li, L.-C. He, and J.-Q. Jin, Phytotherapy Research, vol. 21, no 4, pp. 347?353, 2007).

Garlic ( Pueraria ) lobata (Willd.) Ohwi) is used in oriental medicine as a mechanism to release heat while relaxing muscles. The main chemical components are puerarin and daidzin, which are isoflavones. In some studies on cancer cells, it has been reported that the treatment of arrowroot extract or powder inhibited the proliferation of breast cancer cells and reduced the growth of breast cancer tumors.

Paeonia lactiflora Pallas has been reported to have anti-inflammatory and stress ulcer prevention effects, and various studies have been conducted on it. As pharmacological actions, central nervous system inhibition, smooth muscle relaxation, anti-platelet aggregation, anti-inflammatory, anti-stress and anti-convulsant effects, anti-cancer effects, food poisoning-induced bacterial proliferation inhibitory effects, and immune-enhancing effects have been reported.

Ephedra ( Ephedra sinica Stapf.) is used as a medicinal in oriental medicine because it is effective in dissipating wind and cold, and is effective in sweating, chills, headache, asthma, seawater, edema, paralysis of the upper body, skin paralysis, hyperemia, and blood clots.

However, until now, there has been no report on the anti-obesity effect of the broiler chicken, baekchul, galgeun, ear peony and ephedra complex extract, and there is no research on it.

Therefore, the present inventors overcame the limitations of existing drugs developed for the treatment of obesity, and as a result of earnest efforts to develop a new drug with fewer side effects, it was confirmed that the complex extract has an excellent effect in suppressing obesity by promoting body energy consumption. Thus, the present invention was completed.

While the present inventors were studying the effects of broiler chicken, baekchul, galgeun, white peony, and ephedra complex extracts on promoting human energy metabolism, the complex extracts PGC1-α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator- By increasing the expression of activating receptor gamma cofactor-1 alpha) and promoting AMPK (Adenosine monophosphate-activated protein kinase) phosphorylation, it has anti-obesity effect by promoting body energy consumption. By confirming that, the present invention was completed.

Accordingly, it is an object of the present invention to provide a composition containing a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra as active ingredients, having an anti-obesity effect by promoting body energy consumption.

In addition, another object of the present invention is to provide a health food composition containing a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra as active ingredients, which has anti-obesity effect by promoting body energy consumption.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention as described above, the present invention provides a composition containing a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra as an active ingredient, having an anti-obesity effect by promoting body energy consumption.

In one embodiment of the present invention, the complex extract is water, C1 to C4 lower alcohol, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, and a mixed solvent thereof. It is characterized in that it is extracted with one or more solvents selected from the group consisting of.

In another embodiment of the present invention, the composition is characterized in that it increases the expression of peroxisome proliferator-activated receptor gamma cofactor-1 alpha (PGC1-α).

In another embodiment of the present invention, the composition is characterized in that it promotes adenosine monophosphate-activated protein kinase (AMPK) phosphorylation.

In addition, the present invention provides a health functional food composition having an anti-obesity effect through the promotion of body energy consumption containing a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra as active ingredients.

In addition, the present invention is to provide a method of anti-obesity action through the promotion of body energy consumption, including the step of administering to the subject a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra.

In addition, the present invention provides the use of anti-obesity action through the promotion of body energy consumption of the complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra.

It was confirmed that the complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra according to the present invention increases the expression of PGC1-α in muscle and promotes phosphorylation of AMPK, and promotes human energy metabolism, resulting in an imbalance in energy metabolism. It can be utilized for the treatment, prevention, or improvement of obesity, type 2 diabetes, and various metabolic diseases.

1 shows the results of confirming the expression level of PGC1-α in the C2C12 cells treated with the complex extracts of broiler chicken, baekchul, galgeun, white peony and ephedra of the present invention compared with the control group treated with each single extract.
Figure 2 shows the results of confirming the phosphorylation level of AMPK in the C2C12 cells treated with the complex extract of broiler chicken, baekchul, galgeun, ear pea and ephedra of the present invention compared with the control group treated with each single extract.

The present invention provides a composition having an anti-obesity effect through the promotion of body energy consumption containing a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra as active ingredients.

In addition, the present invention provides a health functional food composition having an anti-obesity effect through the promotion of body energy consumption containing a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra as active ingredients.

In one embodiment of the present invention, mouse myoblasts (C2C12 myoblasts) were cultured to induce differentiation into myotubes, and then real-time RT-PCR and western blot experiments were performed (see Example 1). .

In another embodiment of the present invention, a complex extract of broiler chicken, baekchul, galgeun, balsam and ephedra was prepared (see Example 2).

In another embodiment of the present invention, the expression level of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-α) by the complex extract is determined by using each single extract. It was confirmed that there was a significant increase compared to the treated control group (see Example 3).

In another embodiment of the present invention, the phosphorylation level of adenosine monophosphate-activated protein kinase (AMPK) by the complex extract was significantly increased compared to the control group treated with each single extract. It was confirmed (see Example 4).

Therefore, in view of the above results, the complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra according to the present invention increases PGC1-α expression and AMPK phosphorylation level in muscle cells, a disease caused by mitochondrial dysfunction, that is, It can be used as a medicine useful for metabolic diseases such as obesity and diabetes caused by lack of energy metabolism or aging-related diseases, and as a health functional food with anti-obesity effect by promoting body energy consumption.

The complex extract of broiler chicken, baekchul, galgeun, peony and ephedra of the present invention can be prepared by a conventional method known in the art, that is, under normal temperature and pressure conditions, using a conventional solvent. As an extraction solvent that can be used for the preparation of the complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra of the present invention, for example, water, C1 to C4 lower alcohol, n-hexane, ethyl acetate, acetone, butyl acetate, 1 Extraction solvents such as ,3-butylene glycol, methylene chloride, and mixed solvents thereof may be used alone or in combination, and preferably water, but is not limited thereto. A suitable amount of the extraction solvent is about 1 to 10 times the dry weight of broiler chicken, baekchul, brown root, oyster root and ephedra, and heat extraction, cold extraction, reflux cooling extraction, ultrasonic extraction, etc. Alternatively, it can be used by repeatedly extracting multiple times. In addition, the extraction temperature is not particularly limited, as long as the effective activity of the useful ingredients of broiler chicken, baekchul, galgeun, ear pea, and ephedra is not removed, but is preferably extracted by immersion at room temperature.

The composition of the present invention may contain one or more known active ingredients having an effect of inhibiting obesity through the promotion of body energy consumption together with a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra.

As used herein, the term “administration” means providing a given composition of the present invention to a subject by any suitable method.

The composition of the present invention may be prepared by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration. The pharmaceutically acceptable carrier may be used in a mixture of saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components. , and other conventional additives such as a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets. Furthermore, it can be preferably formulated according to each disease or component by an appropriate method in the art.

The composition of the present invention may be administered orally or parenterally (eg, intravenously, subcutaneously, intraperitoneally or topically) according to a desired method, and the dosage may vary depending on the patient's weight, age, sex, and health status. , diet, administration time, administration method, excretion rate and the severity of the disease, etc., the range varies. The daily dose of the complex extract is about 0.0001-500 mg/kg, preferably about 0.001-300 mg/kg, and it is preferably administered once or several times a day.

The composition of the present invention may be used alone or in combination with methods using surgery, hormone therapy, drug therapy, and biological response modifiers for the effect of suppressing obesity through promotion of body energy consumption.

In the present invention, "health functional food" refers to food having body control functions such as prevention and improvement of diseases, body defense, immunity, recovery from illness, and suppression of aging, and should be harmless to the human body when taken for a long time.

The composition of the present invention may be added to a health functional food for the purpose of suppressing obesity through the promotion of body energy consumption. When the complex extract of broiler chicken, baekchul, galgeun, oyster root, and ephedra of the present invention is used as a food additive, the complex extract of broiler chicken, baekchul, galgeun, earl yam, and ephedra can be added as it is or used with other foods or food ingredients, It can be used suitably according to a conventional method. The mixed amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, in the production of food or beverage, the complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less, based on the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of the food. Examples of foods to which the above substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, There are alcoholic beverages and vitamin complexes, and it includes all health functional foods in the ordinary sense.

The health beverage composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients, as in a conventional beverage. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as taumatine and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used. The proportion of the natural carbohydrate is generally about 0.01-0.20 g, preferably about 0.04-0.10 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, It may contain a carbonation agent used in carbonated beverages, and the like. In addition, the composition of the present invention may contain the pulp for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not critical, but is generally selected in the range of 0.01-0.20 parts by weight per 100 parts by weight of the composition of the present invention.

In addition, the present invention provides a method of suppressing obesity through the promotion of body energy consumption, comprising administering to the subject a complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra.

The subject is a mammal including a human or a non-human, and the non-human mammal includes, but is not limited to, a mouse, a rat, a dog, a cat, a horse, a cow, a sheep, a goat, a pig, a rabbit, and the like.

Hereinafter, preferred examples are presented to help the understanding of the present invention. However, the following examples are only provided for easier understanding of the present invention, and the content of the present invention is not limited by the examples.

Example 1: Experiment Preparation

1.1 Cell Culture

The mouse-derived C2C12 cell line was purchased from the American Type Culture Collection (ATCC, CRL-1772; Manassas, VA, USA). To induce differentiation into myotubes by culturing mouse myoblasts (C2C12 myoblasts), each 1×10 ⁶ seeding of C2C12 myoblasts in a 60 mm Petri dish, 10% fetal bovine serum serum, FBS) and high glucose Dulbecco's modified eagle's medium (DMEM; Invitrogen, Grand Island, NY, USA) containing 1% penicillin / streptomycin (penicillin / streptomycin mix 1%) until 100% confluence. cultured.

To induce differentiation, the medium was changed to differentiation medium (DMEM supplemented with 2% horse serum) every 24 hours for 4 days. Each was treated with the same concentration of 0.5 mg / ml for 24 hours.

1.2 Real-time reverse transcriptase Chain Reaction (Real Time Reverse Transcriptase-Polymerase Chain Reaction, Real Time RT-PCR)

Cells were harvested, centrifuged at 5,000 rpm for 5 minutes, and total RNA was isolated using TRIzol reagent. cDNA was synthesized using reverse transcriptase on the isolated RNA. In the synthesized cDNA, SensiFAST SYBR® Lo-ROX mix (Bioline, London, UK) and PGC-1α primer (sense; 5'-CAC CAA ACC CAC AGA AAA CAG-3', antisense; 5'-GGG TCA GAG GAA GAG ATA AAG TTG-3') and GAPDH primer (sense; 5'-ATT CAA CGG CAC ACT CAA GG-3', antisense; 5'-CAG TGT AGC CCA AGA TGC CCT-3') were mixed respectively. This was subjected to real-time chain polymerization in 40 cycles at 95 °C for 5 seconds for denaturation, 10 seconds at 60 °C for annealing, and 20 seconds at 72 °C for extension (Mx300P, Agilent Technologies, CA, USA).

1.3 western blot (Western blot)

The cells were harvested, centrifuged at 5,000 rpm for 5 minutes, washed twice with PBS solution, and lysis solution (50 mM HEPES, pH 7.4, 150 mM NaCl, 1% deoxycholate, 1 mM EDTA, 1 mM PMSF, 1 μg/ ml aprotinin) and then reacted on ice for 30 minutes. 20 μg of protein is mixed with 2× sample buffer (100 mM Tris-HCl, pH 6.8, 200 mM dithithreitol, 4% SDS, 0.2% bromophenol blue, 20% glycerol) and boiled at 100 ° C for 3 minutes, then 8-15% SDS -PAGE was used to separate. The protein in the gel separated through electrophoresis was transferred to a nitrocellulose membrane at 4° C. and 30 V for 16 hours. The membrane was blocked with 10% skim milk at room temperature for 1 hour, and then reacted with the antibody of each protein for 2 hours at room temperature. This was washed 3 times with TBS-T, treated with anti-pAMPK and AMPK (Cell signaling technology, MA, USA), respectively, and reacted at room temperature for 1 hour, followed by substrate reaction with ECL solution, and photosensitized to x-ray film. The band on the X-ray film was expressed as the expression ratio of pAMPK to AMPK using the Image-J program.

Example 2: Broiler chicken, baekchul, galgeun, earl yam and ephedra ( Cinnamomum cassia Blume, Atractylodes macrocephala Koidzumi, Pueraria lobata (Willd.) Ohwi, Paeonia lactiflora Pallas and Ephedra sinica Stepf.) Preparation of complex extracts

After preparing 50 g of each dried broiler chicken, baekchul, brown root, white peony and ephedra, 2.5 L of purified water was added and heated in a hot water extractor for 3 hours to obtain a water extract of the complex component. This was filtered through filter paper (Whatman NO. 1; Sigma-Aldrich, St. Louis, MO, USA) and concentrated under reduced pressure using a rotary vacuum concentrator to prepare dry powder of the composite extract, and the yield was 26.9%. In addition, for the comparative experiment, to prepare each single extract of broiler chicken, baekchul, galgeun, white peony, and ephedra, prepare 200 g each of the dried product, add 2 L of purified water, and heat each in a boiling water extractor for 3 hours. The method was used to prepare a dry powder of each single extract. The yields were 6.7% for broiler chicken, 26.0% for baekchul, 32.0% for galgeun, 40.9% for earl, and 6% for ephedra, respectively.

Example 3: Broiler , White, craving , by the complex extract of earl and ephedra PGC - One Confirmation of expression change of α

PGC-1α is a key factor regulating energy metabolism in cells, and is an important factor in mitochondrial angiogenesis, and plays an important role in fatty acid oxidation and thermogenesis in skeletal muscle. Therefore, in order to confirm the expression of PGC-1α by the complex extract of broiler chicken, baekchul, galgeun, oyster root and ephedra in C2C12 cells, the mRNA expression level of PGC-1α was measured by the real-time RT-PCR method of Example 1-2. .

As a result, as shown in FIG. 1 , it was confirmed that the expression level of PGC-1α was significantly increased during treatment of the broiler chicken, baekchul, galgeun, ear pea, and ephedra complex extract compared to each single extract.

Example 4: Broiler , White, craving , by the complex extract of earl and ephedra AMPK Confirmation of phosphorylation changes in

AMPK is a major enzyme that acts as a sensor in the maintenance of energy homeostasis in cells and increases mitochondrial neoplasia, glucose transport, and fatty acid oxidation in skeletal muscle through phosphorylation. In addition, phosphorylated AMPK acts directly to directly increase PGC-1α activity. Accordingly, the protein expression levels of AMPK and pAMPK were measured by the Western blot method of Examples 1-3.

As a result, as shown in Figure 2, compared to each single extract, it was confirmed that phosphorylation of AMPK was significantly increased when treated with broiler chicken, baekchul, galgeun, oyster root and ephedra complex extract.

As a result of synthesizing the above, it was confirmed that the complex extract of broiler chicken, baekchul, galgeun, ear pea and ephedra of the present invention significantly increased the expression level of PGC-1α and phosphorylation of AMPK in muscle cells, the complex extract It can be seen that this body has an excellent effect on anti-obesity through the promotion of energy consumption.

Hereinafter, formulation examples for the composition of the present invention will be described, but the present invention is not intended to be limited, but only specifically described.

Formulation example 1: Preparation of pharmaceutical formulations

1. Preparation of powder

200mg of complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra

Lactose 100mg

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

2. Preparation of tablets

200mg of complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra

Corn Starch 100mg

Lactose 100mg

Magnesium stearate 2mg

After mixing the above ingredients, tablets were prepared by tableting according to a conventional method for manufacturing tablets.

3. Preparation of capsules

200mg of complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra

Corn Starch 100mg

Lactose 100mg

Magnesium stearate 2mg

After mixing the above ingredients, the capsules were prepared by filling in gelatin capsules according to a conventional manufacturing method of capsules.

4. Preparation of injections

200mg of complex extract of broiler chicken, baekchul, galgeun, earl yam and ephedra

Mannitol 100mg

Na ₂ HPO ₄ 12H ₂ O 2mg

Appropriate amount of sterile distilled water for injection

An injection was prepared by mixing the above ingredients per ampoule (2 ml) according to a conventional injection preparation method.

Formulation example 2: Manufacture of food

Foods containing the complex extract of the present invention were prepared as follows.

1. Preparation of cooking seasoning

A cooking seasoning for health promotion was prepared with 20-95% by weight of the complex extract.

2. Preparation of Tomato Ketchup and Sauce

0.2-1.0 wt% of the complex extract was added to tomato ketchup or sauce to prepare tomato ketchup or sauce for health promotion.

3. Manufacture of wheat flour food

0.5-5.0 wt% of the complex extract was added to wheat flour, and the mixture was used to prepare bread, cake, cookies, crackers, and noodles to prepare health-promoting foods.

4. Preparation of soups and gravies

0.1-5.0 wt% of the complex extract was added to the soup and broth to prepare health-promoting meat products, noodle soups and broths.

5. Preparation of ground beef

10 wt% of the complex extract was added to ground beef to prepare ground beef for health promotion.

6. Manufacture of dairy products

5-10% by weight of the complex extract was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

Formulation example 3: Preparation of beverages

1. Manufacture of carbonated beverages

Complex extract 10-15%, sugar 5-10%, citric acid 0.05-0.3%, caramel 0.005-0.02%, vitamin C 0.1-1% additives were mixed, and 75-80% purified water was mixed to make syrup. . The syrup was sterilized at 85-98° C. for 20-180 seconds, mixed with cooling water in a ratio of 1:4, and then 0.5-0.82% of carbon dioxide was injected to prepare a carbonated beverage containing the complex extract.

2. Manufacture of health drinks

Complex extract (solid content 2.5%, 97.16%), jujube extract (65 brix, 2.67%), fruit complex extract (solid content 70%, 0.12%), vitamin C (0.02%), calcium pantonethenate (0.02%), licorice extract (65% solid content, 0.01%) was homogeneously mixed and instantaneously sterilized, and then packaged in small packaging containers such as glass bottles and plastic bottles to prepare health drinks.

3. Preparation of vegetable juice

By adding 0.5 g of the complex extract to 1,000 ml of tomato or carrot juice, vegetable juice for health promotion was prepared.

4. Preparation of fruit juice

By adding 0.1 g of the complex extract to 1,000 ml of apple or grape juice, fruit juice for health promotion was prepared.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (6)

An anti-obesity pharmaceutical composition containing a complex extract of a mixture consisting of broiler chicken, baekchul, galgeun, ear pea and ephedra as an active ingredient.
According to claim 1,
The complex extract is at least one selected from the group consisting of water, C1 to C4 lower alcohol, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, and a mixed solvent thereof. An anti-obesity pharmaceutical composition, characterized in that extracted with a solvent.
According to claim 1,
The composition is a peroxisome proliferator-activated receptor gamma coactivator-1 alpha (peroxisome proliferator-activated receptor gamma coactivator-1 alpha, PGC-1α) characterized in that to increase the expression, anti-obesity pharmaceutical composition.
According to claim 1,
The composition is adenosine monophosphate-activated protein kinase (Adenosine monophosphate-activated protein kinase, AMPK) characterized in that to promote phosphorylation, anti-obesity pharmaceutical composition.
An anti-obesity health functional food composition containing, as an active ingredient, a complex extract of a mixture consisting of broiler chicken, baekchul, galgeun, ear pea, and ephedra.
6. The method of claim 5,
The complex extract is at least one selected from the group consisting of water, C1 to C4 lower alcohol, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, and a mixed solvent thereof. An anti-obesity health functional food composition, characterized in that it is extracted with a solvent.

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