KR20220006142A - Manufacturing method of cosmetic composition using lactobacillus plantarum fermented water - Google Patents

Abstract

The present invention relates to a method for manufacturing a cosmetic composition using a lactobacillus-fermented Ceramium kondoi water. According to the present invention, the method comprises the following steps of: heating each of a phase A consisting of seawater, a phase B consisting of 1,3-butylene glycol, 1,2-hexanediol, niacinamide, glycerin, and adenosine, and a phase C consisting of lavender oil and polysorbate at 80 ℃ (S10); adding the phase A to the phase B, stirring the mixture, adding the phase C to the mixture, and solubilizing the mixture to form a first composition (S20); adding a phase D consisting of carbomer, hydroxyethyl cellulose, and sodium polyacrylate to the first composition and stirring the mixture to form a second composition (S30); cooling the second composition to room temperature, adding a phase E consisting of hydrolyzed collagen, raffinose, glutamine, lysine, cysteine, arginine, inositol, histidine, and sodium hyaluronate to the second composition and stirring the mixture to form a third composition (S40); and adding a phase F consisting of Lactobacillus-fermented Ceramium kondoi extract to the third composition, and performing dispersing, cooling, and defoaming to acquire a cosmetic composition (S50). Accordingly, an eco-friendly cosmetic composition rich in antioxidants is manufactured.

Description

Method for manufacturing a cosmetic composition using silk grass lactobacillus fermented water {MANUFACTURING METHOD OF COSMETIC COMPOSITION USING LACTOBACILLUS PLANTARUM FERMENTED WATER}

The present invention relates to a method for manufacturing a cosmetic composition using fermented lactobacillus fermented water, and more particularly, an eco-friendly cosmetic composition rich in antioxidants by containing fermented water obtained by fermenting and ripening fermented silk grass using Lactobacillus as an active ingredient. relates to the manufacture of

In general, oxygen that enters the body through respiration generates active oxygen in the process of providing energy necessary for the human body. As is known, free radicals attack normal cells and act as a cause of aging and various diseases.

Antioxidant inhibits cell oxidation to prevent aging. As the age increases, the ability of antioxidant enzymes to remove active oxygen in the body decreases rapidly, so it is necessary to supply antioxidants from the outside. Various technical attempts have been made to contain antioxidant functions.

Representative antioxidants include polyphenols, vitamin C, vitamin E, and beta-carotene. Among them, polyphenol is a major substance that determines antioxidant activity, and catechins in green tea, resveritrol in wine, and flavonoids in fruits and vegetables are a kind of polyphenol.

On the other hand, silk grass is a red-purple plant and contains a large amount of saponins and flavonoids, and is known to have antibacterial and inhibitory effects on harmful bacteria and discharge of toxins and wastes.

As an example of the prior art using the efficacy of such silk grass, Korean Patent Application Laid-Open No. 10 - 2011 - 0120813 A step of pre-treating silk grass with an organic solvent, drying the residue, and using a lower alcohol solvent such as C1-4 ethanol or methanol It constitutes a method for producing an extract of silkworm for wrinkle improvement, which includes the step of extracting and fractionation with a solvent.

As another example, Korean Patent Application Laid-Open No. 10-2019-0028996 discloses UV rays containing a complex extract obtained by using water or a lower alcohol having 5 or less carbon atoms, a persimmon leaf extract, and a silk extract, as an active ingredient. to constitute a cosmetic composition for skin protection against

On the other hand, Lactobacillus is a bacterium that produces a large amount of lactic acid bacteria using the energy obtained by fermenting sugars. Lactobacillus plantarum is a lactic acid bacterium belonging to the genus Lactobacillus, which is widely used as a probiotic strain and is known to inhibit harmful bacteria as well as the proliferation of lactic acid bacteria.

For example, Korean Patent Registration No. 10-1078933 contains a mushroom fermented extract obtained by fermenting mushrooms using lactic acid bacteria of the genus Lactobacillus as an active ingredient. And, the step of sterilizing in a high-pressure steam sterilizer, inoculating and culturing lactic acid bacteria of the genus Lactobacillus, and filtering and freeze-drying the culture to form a pharmaceutical composition for preventing or treating allergic diseases.

As another example, Korean Patent Registration No. 10-1418820 discloses Lactobacillus plantarum GB-LP1 (KCCM 11258P) lactic acid bacteria strain with excellent immune and antiviral activity under anaerobic conditions, moisture content of 40-50 weight It comprises the step of solid fermenting in a medium conditioned with a wealth, wherein the fermentation constitutes a fermentation method of a lactic acid bacteria strain having excellent immune and antiviral activity, which is performed in a medium in which soybean meal and wheat hull are mixed in a ratio of 2:8.

Korean Patent Laid-Open Patent No. 10 - 2011 - 0120813 (2011.11.04) Korean Patent Laid-Open Patent No. 10 - 2019 - 0028996 (2019.03.20) Korean Patent Registration No. 10 - 1078933 (2011.11.01) Korean Patent Registration No. 10 - 1418820 (2014.07.17)

The method for producing a composition using a silk grass extract to which the prior art is applied as described above is made to extract the active ingredient of silk grass using an organic solvent and an alcohol solvent such as ethanol or water.

However, the extraction method using water or a solvent as in the prior art has a disadvantage in that it is difficult to actually expect a great antioxidant function because the extraction rate of the polyphenol component contained in silk grass is low.

In addition, the prior art requires an additional concentration process due to the addition of water or solvent, so the process is complicated and inefficient, and active ingredients having various antioxidant functions other than polyphenols contained in silk grass during the extraction process, such as saponin or beta-carotene, There is a problem that cannot be excluded from the denaturation or loss of nutrients such as various vitamins, potassium, and calcium.

Accordingly, the present invention was invented to solve the problems of the prior art as described above,

Phase A consisting of seawater, phase B consisting of 1,3-butylene glycol, 1,2-hexanediol, niacinamide, glycerin, and adenosine, and phase C consisting of lavender oil and polysorbate 80 were respectively heated at 80 °C. A step of warming with (S10) and,

After adding and stirring the A phase to the B phase, adding and solubilizing the C phase to form a first composition (S20);

A step (S30) of adding and stirring phase D consisting of carbomer, hydroxyethyl cellulose, and sodium polyacrylate to the first composition to form a second composition (S30);

After cooling the second composition to room temperature, adding phase E consisting of hydrolyzed collagen, raffinose, glutamine, lysine, cysteine, arginine, inositol, histidine, and sodium hyaluronate and stirring to form a third composition ( S40) and

By including the step (S50) of adding and dispersing, cooling and defoaming, phase F consisting of the Lactobacillus silk ferment extract to the third composition to obtain a cosmetic composition (S50), which is rich in antioxidants and an eco-friendly cosmetic composition for the purpose of producing It is possible to achieve

The present invention provides a method for producing a cosmetic composition containing fermented water fermented and aged silk grass using Lactobacillus.

Therefore, the present invention has the advantage of being able to streamline the process and minimize the loss of other active ingredients compared to the conventional method of extracting and concentrating silk grass using water or a solvent.

In particular, the present invention is made to more effectively extract silk grass containing a large amount of antioxidants such as polyphenols and beta-carotene using Lactobacillus, thereby significantly improving the extraction rate of polyphenols compared to the prior art and promoting the inhibitory effect of SOD-like activity By doing so, there is an effect of producing a cosmetic composition rich in antioxidants and eco-friendly.

1 and 2 are schematic process flow charts of a method for manufacturing a cosmetic composition using fermented lactobacillus fermented water according to the present invention.
3 to 5 are graphs showing the results of Experimental Examples 1 to 3 of the efficacy of the Lactobacillus silk fermented extract, the Lactobacillus baekbokryeong fermented extract, the Lactobacillus sukjihwang fermented extract, and the Lactobacillus licorice fermented extract according to the present invention; .

Hereinafter, the configuration and action according to a preferred embodiment of the method for manufacturing a cosmetic composition using the silk grass lactobacillus fermented water of the present invention will be described in detail with reference to the accompanying drawings. In the following description, detailed descriptions of parts that can be easily implemented by those skilled in the art may be omitted. In addition, since the following description is given with reference to preferred embodiments of the present invention, the present invention is not limited by the following examples, and it is natural that various modifications may be provided without departing from the scope of the present invention. something to do.

1 and 2 show a schematic process flow diagram of a method for manufacturing a cosmetic composition using fermented lactobacillus fermented water according to the present invention. Efficacy of the fermentation extract, the Lactobacillus sukhumus fermented extract, and the fermented Lactobacillus licorice extract It is a graph showing the implementation results of Experimental Examples 1 to 3.

It should be noted that the method for producing a cosmetic composition using fermented water of lactobacillus silkworm to which the technology of the present invention is applied contains fermented water obtained by fermenting and ripening silkgrass using Lactobacillus, it is noted that it relates to the preparation of an eco-friendly cosmetic composition rich in antioxidants. .

For this purpose, the method for manufacturing a cosmetic composition using the fermented water of the silkworm lactobacillus of the present invention comprises the steps of heating the A phase, B phase, and C phase (S10) as shown in FIGS. A step (S20), a step of composing a second composition (S30), a step of composing a third composition (S40), and a step of obtaining a cosmetic composition (S50) are included, and are specifically as follows. .

The step of warming the A phase, B phase, and C phase (S10) is a phase A consisting of seawater, and phase B consisting of 1,3-butylene glycol, 1,2-hexanediol, niacinamide, glycerin, and adenosine. It is a step of heating each of the phase C consisting of lavender oil and polysorbate 80 to 80°C.

The A phase consists of 39.75 parts by weight of seawater based on the total weight of the cosmetic composition.

The phase B contains 2.3 parts by weight of 1,3-butylene glycol, 2.00 parts by weight of 1,2-hexanediol, 2.00 parts by weight of niacinamide, 1.70 parts by weight of glycerin, and 0.50 parts by weight of adenosine, based on the total weight of the cosmetic composition. to create

The 1,3-butylene glycol and niacinamide have a skin conditioning function, and the 1,2-hexanediol has a natural moisturizing, antibacterial and antifungal function. The glycerin is effective in moisturizing and skin diseases, and the adenosine has a skin conditioning and wrinkle improvement function.

The C phase is formulated by mixing 1.00 parts by weight of lavender oil and 0.05 parts by weight of polysorbate 80 with respect to the total weight of the cosmetic composition.

The lavender oil has a flavoring and skin conditioning function, and the polysorbate 80 has a flavoring and emulsifying function.

The step of composing the first composition (S20) is a step of adding the A phase to the B phase and stirring, then adding the C phase and solubilizing it to form a first composition.

The step of composing the second composition (S30) is a step of adding and stirring phase D consisting of carbomer, hydroxyethyl cellulose, and sodium polyacrylate to the first composition to form a second composition.

The D phase is formulated by mixing 0.10 parts by weight of carbomer, 1.20 parts by weight of hydroxyethyl cellulose, and 0.80 parts by weight of sodium polyacrylate based on the total weight of the cosmetic composition.

The carbomer has an emulsion stability and thickening function, and the hydroxyethylcellulose has a binder and film-forming function. The sodium polyacrylate has functions of absorbent, skin softening, and film formation.

In the step of composing the third composition (S40), after cooling the second composition to room temperature, hydrolyzed collagen, raffinose, glutamine, lysine, cysteine, arginine, inositol, histidine, and E phase consisting of sodium hyaluronate It is a step of adding and stirring to form a third composition.

The E phase is formulated by mixing 2.80 parts by weight of hydrolyzed collagen, 3.30 parts by weight of sodium hyaluronate, and 13.50 parts by weight of a mixture of raffinose, glutamine, lysine, cysteine, arginine, inositol, and histidine with respect to the total weight of the cosmetic composition.

The hydrolyzed collagen and the sodium hyaluronate have a skin conditioning function. Since the raffinose, glutamine, lysine, cysteine, arginine, inositol, and histidine are additives commonly used in cosmetic compositions, specific characteristics may refer to well-known techniques.

The step of obtaining the cosmetic composition (S50) is a step of obtaining a cosmetic composition by adding, dispersing, cooling and defoaming phase F made of the Lactobacillus silk fermented extract to the third composition.

The F phase consists of 12.00 parts by weight of the Lactobacillus silk fermented extract, or 12.00 parts by weight of the Lactobacillus silkworm ferment extract, 5.50 parts by weight of the Lactobacillus baekbokryeong fermented extract, 6.50 parts by weight of the Lactobacillus Sukhumus fermented extract, lactobacillus relative to the total weight of the cosmetic composition. It is prepared by mixing one or more selected from 5.00 parts by weight of Bacillus licorice ferment extract.

The silk grass contains a large amount of flavonoid components and saponins, which are a kind of polyphenols such as beta-carotene, vitamins A and C, and has antioxidant and inhibitory functions of harmful bacteria. The baekbokryeong contains a large amount of beta-glucan and has an immune-enhancing function, and the sukjihwang has a nourishing and tonic function by containing pachymic acid, which is a kind of naturally-derived steroid. The licorice contains a large amount of triterpenoid, coumarin, and flavonoid components to have anticancer and antioxidant functions. The silk grass, baekbokryeong, sukjihwang, and licorice may cause side effects when overdose, so they should be formulated within the above formulation range.

The Lactobacillus silkworm ferment extract, Lactobacillus baekbokryeong fermentation extract, Lactobacillus sukjihwang fermented extract, and Lactobacillus licorice fermented extract were respectively added to the liquid medium inoculated with Lactobacillus plantarum and licorice powder, respectively. Cultivation at 40° C. for ~50 hours is achieved by fermentation aging.

The Lactobacillus is a bacterium that produces a large amount of lactic acid bacteria by using the energy obtained by fermenting sugars. In the step of obtaining the cosmetic composition (S50), the silk fermented extract fermented by Lactobacillus planterum is used alone as phase F, or one or more selected from among baekbokryeong, sukjihwang, and licorice fermented extracts to extract the fermented silkensis It is mixed with and configured to be used as phase F.

Hereinafter, an embodiment including the present invention having the configuration as described above will be configured and the effects thereof will be understood in detail.

<Example 1>

According to the method for manufacturing a cosmetic composition using fermented silk grass lactobacillus water according to the present invention, the step of heating the A phase, the B phase, and the C phase (S10), the step of composing the first composition (S20), and the second composition A cosmetic composition is prepared through a process consisting of a composition step (S30), a step of forming a third composition (S40), and a step of obtaining a cosmetic composition (S50). Table 1 below describes the compounding ratio of the ingredients to be added in each step. In particular, in the phase F to be added in the step (S50) of obtaining the cosmetic composition, silk grass, baekbokryeong, sukjihwang, and licorice powder were added to the liquid medium inoculated with Lactobacillus plantarum, respectively, and cultured at 40°C for 48 hours to ferment and ripen. and Lactobacillus silk grass extract, Lactobacillus baekbokryeong extract, Lactobacillus sukjihwang extract, and Lactobacillus licorice extract are obtained.

Ingredient name Content (parts by weight) A prize sea water 39.75

B prize 1,3-Butylene glycol 2.30 1,2-Hexandiol 2.00 Niacinamide 2.00 Glycerin 1.70 Adenosine 0.50 C prize Lavandula Angustifolia (Lavender) Oil 1.00 Polysorbate 80 0.05
D prize Carbomer 0.10 Hydroxyethylcellulose 1.20 Sodium Polyacrylate 0.80

E prize Hydrolyzed Collagen 2.80 Raffinose, Glutamin, Lysine, Cysteine, Arginine, Inositol, Histidine 13.50 Sodium Hyaluronate 3.30

F award Fermented euphoribia supina of Lactobacillus plantarum 12.00 Fermented white poria cocoa of Lactobacillus plantarum 5.50 Fermented rehmanneh glutinosa Libosch euphoribia supina of Lactobacillus plantarum 6.50 Fermented glycyrrhiza uralensis Fischer of Lactobacillus plantarum 5.00

<Example 2>

As a comparative group of the present invention, extracts are prepared by solvent extraction, hot water extraction, and ultrasonic extraction of silk grass according to the prior art, and the manufacturing method is as follows.

First, each of the silk grass, baekbongryeong, sukbonghwang, and licorice is washed in running water, dried with hot air, and pulverized into fine powder and stored at -20°C.

To each of the fine powders, 70% ethanol of 20 times the volume of the fine powder was added, stirred at 250 rpm for one day, and then the supernatant was separated using a centrifuge at 5000 rpm for 5 minutes, and extracted three times to prepare a dandelion solvent extract do.

To each of the fine powders, water 10 times the volume of the fine powder is added, and after extraction at 90° C. for 3 hours using a reflux condenser, the supernatant is separated, and extracted 3 times to prepare a hot water extract of silkworm.

To each of the fine powders, purified water 20 times the volume of the fine powder is added, the cell wall is destroyed using an ultrasonic extractor, and the useful substances are separated while stirring at low temperature for 1 hour. After drying the separated useful substances in a vacuum evaporator or a dryer at 60 ~ 65 ℃, a fixed amount is dissolved in an extraction solvent to prepare an ultrasonic extract of silkworm.

<Experimental Example 1>

The total polyphenol content of the Lactobacillus silk fermented extract of the present invention according to Example 1, the Lactobacillus baekbokryeong fermented extract, the Lactobacillus sukjihwang fermented extract, and the Lactobacillus licorice fermented extract was measured.

Phenolic compounds are one of the secondary metabolites widely distributed in the plant kingdom, and have various molecular structures and molecular weights. Because they have a phenolic hydroxyl (OH) group, they easily combine with proteins and other macromolecules to have various physiological activities such as antioxidant and anticancer. In this experiment, the content of polyphenol compounds was compared using gallic acid as a standard curve to measure the total polyphenol content present in the fermented extracts and fractions of silk grass, and the results are as shown in the graph of FIG. 3 below.

As for the polyphenol content, 65.03mg GAE/g of silk fermented extract, 38,9mg GAE/g of Baekbongryeong fermented extract, 62.2mg GAE/g of fermented Sukhumi extract, and 37.3mg GAE/g of licorice fermented extract were measured. It was confirmed that it had the highest polyphenol content at 65.03 mg GAE/g. Therefore, in the step (S50) of obtaining the cosmetic composition of the present invention, the silk fermented extract fermented by Lactobacillus planterum is used alone as phase F, or one or more of baekbokryeong, Sukjihwang, and licorice fermented extracts are used. The content of polyphenols and other active ingredients can be adjusted by selecting it and mixing it with the fermented silk grass extract and using it as phase F.

<Experimental Example 2>

The SOD-like activity inhibitory effect of the Lactobacillus silk fermented extract, the Lactobacillus baekbokryeong fermented extract, the Lactobacillus sukjihwang fermented extract, and the Lactobacillus licorice fermented extract of the present invention according to Example 1 was measured.

SOD inhibitory activity is one of the most important antioxidant enzymes, and SOD plays a role in protecting cells from toxicity by converting superoxide ions into oxygen and hydrogen peroxide. The SOD-like activity inhibitory effect was measured in 500 ug/ml of fermented silkworm extract, fermented baekbongryeong extract, fermented Sukhumang extract, and fermented licorice extract, and the results are as shown in the graph of FIG. 4 below.

In inhibition of SOD-like activity, 66.5% of fermented silkworm, 38.52% of fermented baekbongryeong, 62.3% of fermented Sukhumang, and 36.96% of fermented licorice, fermented silkworm showed 66.5% activity inhibition, which is the control group vitamin. An excellent inhibitory effect was also confirmed compared to the 64.67% activity inhibitory effect of C. Therefore, in the step (S50) of obtaining the cosmetic composition of the present invention, the silk fermented extract fermented by Lactobacillus planterum is used alone as phase F, or one or more of baekbokryeong, Sukjihwang, and licorice fermented extracts are used. By selecting it, it can be mixed with the fermented silk fermented extract and used as phase F to control the effect of inhibiting SOD-like activity.

<Experimental Example 3>

The polyphenol content contained in the Lactobacillus silk fermented extract of the present invention according to Example 1, the solvent extract of silkworm according to the prior art, the hot water extract of silkworm, and the ultrasonic extract of silkworm according to Example 2 was measured, and the results were As shown in the graph of FIG. 5 below.

The conventional silkworm solvent extract was 20.66mg TAE/g, the silkworm hot water extract was 35.95mg TAE/g, the silkworm ultrasonic extract was 42.01mg TAE/g, and the fermented silkworm extract of the present invention showed 65.03mg TAE/g, the present invention It was confirmed that the polyphenol content of the fermented silk grass fermented product was significantly higher than that of the conventional extracts.

As described above, in the method for manufacturing a cosmetic composition using fermented water of silk lactobacillus according to the present invention, silk grass containing a large amount of antioxidants such as polyphenols and beta-carotene is extracted and concentrated using water or a solvent as in the prior art. By differentiating from the method used to extract lactobacilli more effectively, it has the advantage of making the process more efficient and minimizing the loss of other active ingredients compared to the prior art, as well as significantly improving the extraction rate of polyphenol components and similar to SOD. There is an advantage in that by promoting the activity inhibiting effect, it is rich in antioxidants and to prepare an eco-friendly cosmetic composition.

S10: A step of heating phase A, phase B, and phase C
S20: Step of composing a first composition
S30: forming a second composition
S40: Step of composing a third composition
S50: obtaining a cosmetic composition

Claims (9)

Phase A consisting of seawater, phase B consisting of 1,3-butylene glycol, 1,2-hexanediol, niacinamide, glycerin, and adenosine, and phase C consisting of lavender oil and polysorbate 80 were each heated at 80 ° C. A step of warming with (S10) and,
After adding and stirring the A phase to the B phase, adding and solubilizing the C phase to form a first composition (S20);
A step (S30) of adding phase D consisting of carbomer, hydroxyethyl cellulose, and sodium polyacrylate to the first composition and stirring to form a second composition;
After cooling the second composition to room temperature, adding phase E consisting of hydrolyzed collagen, raffinose, glutamine, lysine, cysteine, arginine, inositol, histidine, and sodium hyaluronate and stirring to form a third composition ( S40) and
A method for producing a cosmetic composition using fermented Lactobacillus lactobacillus, characterized in that it comprises the step of dispersing, cooling and defoaming to obtain a cosmetic composition (S50) by adding, dispersing, and cooling and defoaming phase F made of the Lactobacillus fermented extract to the third composition. . The method of claim 1,
The A-phase is a method for manufacturing a cosmetic composition using fermented water of lactobacillus silk, characterized in that it consists of 39.75 parts by weight of seawater based on the total weight of the cosmetic composition. The method of claim 1,
The phase B contains 2.3 parts by weight of 1,3-butylene glycol, 2.00 parts by weight of 1,2-hexanediol, 2.00 parts by weight of niacinamide, 1.70 parts by weight of glycerin, and 0.50 parts by weight of adenosine with respect to the total weight of the cosmetic composition. A method for manufacturing a cosmetic composition using fermented water of Lactobacillus silkworm, characterized in that the composition is made by The method of claim 1,
The C phase is a method for producing a cosmetic composition using fermented lactobacilli water, characterized in that it is formulated by mixing 1.00 parts by weight of lavender oil and 0.05 parts by weight of polysorbate 80 with respect to the total weight of the cosmetic composition. The method of claim 1,
The D phase is a cosmetic composition manufacturing method using fermented water of lactobacillus silk, characterized in that it is formulated by mixing 0.10 parts by weight of carbomer, 1.20 parts by weight of hydroxyethyl cellulose, and 0.80 parts by weight of sodium polyacrylate with respect to the total weight of the cosmetic composition. The method of claim 1,
The E phase comprises 2.80 parts by weight of hydrolyzed collagen, 3.30 parts by weight of sodium hyaluronate, and 13.50 parts by weight of a mixture of raffinose, glutamine, lysine, cysteine, arginine, inositol, and histidine with respect to the total weight of the cosmetic composition. A method for manufacturing a cosmetic composition using fermented water of silk grass Lactobacillus. The method of claim 1,
The F phase consists of 12.00 parts by weight of Lactobacillus silk fermented extract based on the total weight of the cosmetic composition, or
It is characterized in that the composition is composed by mixing 12.00 parts by weight of Lactobacillus silk fermented extract, 5.50 parts by weight of Lactobacillus baekbokryeong fermented extract, 6.50 parts by weight of Lactobacillus sukhumang fermented extract, and 5.00 parts by weight of Lactobacillus licorice fermented extract. A method for manufacturing a cosmetic composition using fermented water of silk grass Lactobacillus. 8. The method of claim 7,
The Lactobacillus silk fermented extract, Lactobacillus baekbokryeong fermented extract, Lactobacillus Sukhumus fermented extract, Lactobacillus licorice fermented extract,
Silkgrass, baekbokryeong, Sukhumbhuang, and licorice powder were added to the liquid medium inoculated with Lactobacillus plantarum, respectively, and cultured at 40°C for 45 to 50 hours to obtain by fermentation and maturation. Using fermented water A method for manufacturing a cosmetic composition. A cosmetic composition using fermented water of Lactobacillus silkworm, characterized in that it is prepared according to any one of claims 1 to 8.

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