KR20160140021A - Composition for whitening containing fermented spotted spurge - Google Patents
Composition for whitening containing fermented spotted spurge Download PDFInfo
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- KR20160140021A KR20160140021A KR1020150076080A KR20150076080A KR20160140021A KR 20160140021 A KR20160140021 A KR 20160140021A KR 1020150076080 A KR1020150076080 A KR 1020150076080A KR 20150076080 A KR20150076080 A KR 20150076080A KR 20160140021 A KR20160140021 A KR 20160140021A
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- South Korea
- Prior art keywords
- ground
- whitening
- composition
- fermented product
- present
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The present invention relates to a whitening composition comprising a fermented product of a natural substance, and comprises a fermented product of a groundhaft as an active ingredient.
The composition for whitening according to the present invention can be usefully used as a cosmetic composition or a health food composition for whitening skin and can be usefully used for preventing or treating diseases such as melanoma which develops due to hypermelanism, And has skin astringent effect. Therefore, it can be effectively used for prevention of aging of skin and improvement of elasticity. Also, it does not cause side effects and does not cause cytotoxicity even when it is used for a long period of time.
Description
The present invention relates to a composition comprising a fermentation product of a natural substance.
Skin melanogenesis is a defense mechanism against the stimulation of ultraviolet rays in melanocyte-producing cells (melanocyte), and melanin-producing activity is increased, and a large amount of melanin thus produced is transferred to keratinocyte, Results. The purpose of the production of melanin may be skin protection, but skin hyperpigmentation due to melanin production can cause spots, freckles, aging pigmentation spots and the like, which can lead to cosmetic discomfort and negative mental effects to the party.
Melanin is known to be produced by nonenzymatic oxidation after tyrosine, an amino acid, is converted to DOPA or dopaquinone by an enzyme called tyrosinase.
It is an object of the present invention to provide a composition comprising a fermentation product of a natural substance having a whitening effect.
The above-described object of the present invention is achieved by providing a whitening cosmetic composition comprising a ground-vinus fermented product as an active ingredient.
The above-described object of the present invention can be attained by providing a pharmaceutical composition for whitening comprising a fermented product of ground vats as an active ingredient.
On the other hand, the object of the present invention described above can be achieved by providing a whitening health food composition comprising a ground-vinous fermented product as an active ingredient.
According to the composition of the present invention, it is possible to control the mechanisms known to be involved in the production of melanin, thereby inhibiting melanogenesis, specifically inhibiting the activity of tyrosinase and inhibiting the expression of MITF Can be suppressed, and melanin production can be fundamentally suppressed. Accordingly, the composition according to the present invention can be effectively used as a cosmetic composition or a health food composition for skin whitening, and can be effectively used for preventing or treating diseases such as melanoma that develops due to hypermelanism.
Further, according to the composition of the present invention, since it has an antioxidative effect and a skin astringent effect in addition to the whitening effect, it can be effectively used to prevent skin aging and improve elasticity.
Further, according to the composition of the present invention, it contains no noxious effects and does not cause cytotoxicity even when it is used for a long period of time, including a naturally derived component as an active ingredient.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of confirming the antioxidative effect of a ground-band fermented product according to an embodiment of the present invention. FIG.
FIG. 2 is a graph showing a result of observing skin astringent effect of a ground-band fermented product according to an embodiment of the present invention. FIG.
FIG. 3 is a graph showing the results of confirming that the ground-band fermented product according to the example of the present invention inhibits the production of melanin. FIG.
FIG. 4 is a graph showing the result of confirming inhibition of tyrosinase by the ground-band fermented product according to the embodiment of the present invention. FIG.
FIG. 5 is a graph showing the results of confirming that the ground-band fermented product according to the embodiment of the present invention inhibits the expression of MITF, which is a gene involved in melanin production.
As used herein, the term " groundbreaking " includes without limitation any plant having the Korean name " Euphorbia " Specifically, "the earth bed bugs" herein is ground bed bugs (Euphorbia humifusa , Euphorbia maculata or Euphorbia supina ), but are not limited thereto.
As used herein, the term " fermentation " means a process in which microorganisms decompose organic matter, such as ground wastes, and is not particularly limited as long as it is known in the art to continuously enable fermentation of ground wastes. There is no limitation on the kind of the microorganism that can be used in the fermentation process of the present invention, and any microorganism that enables the fermentation of a plant known in the art can be applied without limitation. Specifically, the microorganism may include fungi, yeast, More specifically, the microorganism may be Lactobacillus sakei , which is known as Kimchi lactic acid bacteria. Examples of the fermentation process of the present invention include, but are not limited to, the following. For example, the fermentation process of the present invention can be carried out by mixing a solution containing microorganisms at a concentration of 5 to 15%, such as purified water, and ground wastes, and cultivating the same at a temperature of 25 to 35 DEG C for 2 to 4 days.
As used herein, the term " fermented product " is intended to encompass, without limitation, the shape and properties thereof, without limitation, products obtained during the fermentation process of the ground wastes or after the fermentation process is completed.
As used herein, the term " active ingredient "alone refers to an ingredient that exhibits the desired activity or that can exhibit activity with a carrier that is not itself active.
As used herein, the term " whitening " means whitening of the skin and can be understood as a result of inhibiting melanin production. As used herein, the term " whitening " may specifically refer to the prevention, treatment and / or amelioration of symptoms or diseases caused by the production of melanin, such as spots, freckles, melanomas and the like. In the present specification, the term " whitening " may mean inhibiting or inhibiting melanin production by regulating genes, proteins, and the like that are involved in melanin production.
FIELD OF THE INVENTION The present invention relates to a whitening composition comprising a ground-vinegar fermented product as an active ingredient, and more particularly to a whitening cosmetic composition.
In the cosmetic composition according to the embodiment of the present invention, the ground-wing fermented product has an advantage that it can inhibit and inhibit the production of melanin. Specifically, the ground-band fermented product inhibits or inhibits the activity of tyrosinase, inhibits or inhibits the production and activity of DOPA (3,4-dihydroxyphenylalanine), inhibits microphthalmia-associated transcription factor (MITF) ) To inhibit melanin production and thus can be usefully used for skin whitening. In addition, the ground-wing fermented product has an advantage that it is superior in inhibiting the melanin production than the ground-band extract obtained according to the extraction method common in the art.
The formulation of the cosmetic composition according to the embodiment of the present invention is not particularly limited and can be appropriately selected according to the purpose. For example, a lotion (lotion and milk lotion), a lotion lotion, a nutrition lotion, an essence, a nutrition cream, a massage cream, a pack, a gel, an essence, an eye cream, an eye essence, a cleansing cream, a cleansing foam, a cleansing water, , Body cream, body oil, and body essence, but the present invention is not limited thereto. In addition, the cosmetic composition according to an embodiment of the present invention may be used as a formulation of external preparation for skin in the form of ointment, patch, or the like.
The cosmetic composition according to an embodiment of the present invention may be provided in all formulations suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.
The cosmetic composition according to an embodiment of the present invention may contain other ingredients that can synergistically affect the main effect, within the range not impairing the main effect, in addition to the above-mentioned materials. In addition, the cosmetic composition according to an embodiment of the present invention may further include a moisturizing agent, an emollient agent, an ultraviolet absorber, a preservative, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The amount thereof may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, have.
A whitening composition according to another embodiment of the present invention relates to a whitening pharmaceutical composition comprising a ground-band fermented product as an active ingredient.
In the pharmaceutical composition according to the embodiment of the present invention, the ground-wing fermented product has an advantage of inhibiting and inhibiting the production of melanin. Specifically, the ground-band fermented product inhibits or inhibits the activity of tyrosinase, inhibits or inhibits the production and activity of DOPA (3,4-dihydroxyphenylalanine), inhibits microphthalmia-associated transcription factor (MITF) ) To inhibit the production of melanin and thus can be effectively used for the prevention and treatment of diseases caused by melanogenesis such as melanoma. In addition, the ground-wing fermented product has an advantage that it is superior in inhibiting the melanin production than the ground-band extract obtained according to the extraction method common in the art.
When a pharmaceutical composition for whitening according to an embodiment of the present invention is applied to a pharmaceutical product, the pharmaceutical composition may be used as an active ingredient, and an inorganic or organic carrier may be added thereto to form an oral or parenteral dosage form in solid, semi-solid or liquid form It can be formulated into a zero.
Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.
The pharmaceutical composition according to an embodiment of the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.
The effective ingredients of the pharmaceutical composition according to the embodiment of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.
The present invention relates to a whitening composition for whitening comprising a fermented product of ground vats as an active ingredient.
In the health food composition according to the embodiment of the present invention, the ground-band fermented product has an advantage that it can inhibit and inhibit the production of melanin. Specifically, the ground-band fermented product inhibits or inhibits the activity of tyrosinase, inhibits or inhibits the production and activity of DOPA (3,4-dihydroxyphenylalanine), inhibits microphthalmia-associated transcription factor (MITF) ), And thus melanogenesis can be fundamentally blocked, so that it can be effectively used for prevention and improvement of symptoms and diseases caused by melanin production, such as spots, freckles, melanomas, and the like. In addition, the ground-wing fermented product has an advantage that it is superior in inhibiting the melanin production than the ground-band extract obtained according to the extraction method common in the art.
The health food composition according to an embodiment of the present invention provides various types of food additives or functional foods. It can be processed into an extruded tea, a liquid tea, a beverage, a fermented milk, a cheese, a yogurt, a juice, a probiotic agent and a health supplement food including the above health food composition, and can be used in various other food additives.
The health food composition according to the embodiment of the present invention may contain other components or the like which can give synergy to the main effect within a range not impairing the intended main effect of the present invention. For example, additives such as a perfume, a coloring agent, a bactericide, an antioxidant, an antiseptic, a moisturizer, a thickening agent, an inorganic salt, an emulsifier and a synthetic polymer material may be further added for improving physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.
The health food composition according to an embodiment of the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc. It may be administered by various methods such as simple drinking, injection, spraying or squeezing .
In the whitening composition according to the embodiment of the present invention, the ground-vinous fermentation product is classified into Euphorbia humifusa , Euphorbia maculata , and Euphorbia supina . < Desc / Clms Page number 7 >
In addition, in the composition for whitening according to an embodiment of the present invention, the ground vinifera may be a ground bed and a fermentation product of microorganisms.
Specifically, in the whitening composition according to an embodiment of the present invention, the microorganism may include at least one selected from the group consisting of fungi, yeast, and lactic acid bacteria. More specifically, the microorganism may be selected from the group consisting of Lactobacillus sakei . For example, in the composition for whitening according to the embodiment of the present invention, the ground-vane fermentation product may be a ground wastewater and a fermentation product of Lactobacillus sakei .
In the composition for whitening according to an embodiment of the present invention, the composition may contain 0.001 to 10% by weight of the ground-band fermented product. When the whitening composition according to an embodiment of the present invention contains less than 0.001% by weight of the ground-up fermented product, the skin whitening effect is insignificant. When the whitening composition contains more than 10% by weight, Which is not suitable. In view of the above, the composition for whitening according to an embodiment of the present invention may contain 0.005 to 9.5 wt%, 0.01 to 9 wt%, 0.05 to 8.5 wt%, 0.1 to 8 wt%, or 0.5 to 7.5 wt% As shown in FIG.
Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It is to be understood, however, that the description and examples of the present invention are provided for the purpose of illustration and are in no way to be taken to limit the scope of the present invention. It does not mean that it is limited.
[ Example One] Groundbreaker Fermented product ( ESR -F)
Euphorbia supina ) was dried well and then shredded with a crusher. Lactobacillus sakei was cultivated in 20% skim milk and the culture broth was used as lactic acid bacteria in the fermentation process. The culture was added to purified water at a concentration of 10%, mixed with the crustacean of the petioles, and cultured at 30 DEG C for 3 days to obtain a ground-bed fermentation product. The ground-vinegar fermented product was filtered and lyophilized to be used in the following Experimental Examples.
[ Comparative Example One] Groundbreaker extract( ESR -E)
The crustacea obtained in Example 1 was inoculated into ethanol and extracted at 80 ° C for 4 hours to obtain a ground band extract. The glandular extract was also filtered and then lyophilized to be used in the following Experimental Examples.
[ Experimental Example One] Groundbreaker Fermented product ( ESR -F) Antioxidant ability evaluation
To investigate the antioxidant effect of the ground-band fermented product prepared in Example 1, reduction of the organic radical DPPH (1,1-diphenyl-2-picryl hydrazyl) , The DPPH oxidation inhibitory effect was evaluated. L-ascorbic acid (L-A), an antioxidant known in the art, was used as another positive control, together with the ground-band extract (ESR-E) prepared in Comparative Example 1. Experiments were carried out using 100 μl / ml, 50 μl / ml, 25 μl / ml and 12.5 μl / ml, respectively.
Each test sample was diluted to the above concentration, and then 60 μl of the sample was mixed with 240 μl of 0.2 mM DPPH (2,2-diphenyl-1-picrylhydrazyl). The mixture was allowed to stand at 37 ° C. for 30 minutes and absorbance at 517 nm Respectively. Blank was used as a dilution solvent for the sample, and DPPH reagent was used as a negative control dilution solvent.
1, it was confirmed that the DPPH radical was eliminated in a concentration-dependent manner by the ground-fed fermented product (ESR-F). Thus, it was confirmed that the ground-vinegar fermented product had antioxidant ability. In addition, FIG. 1 shows that the groundbreaking fermented product, which is an effective ingredient of the composition according to the embodiment of the present invention, is superior in antioxidant activity to the ground-band extract (ESR-E).
[ Experimental Example 2] Groundbreaker Fermented product ( ESR -F) skin elasticity Improvement ability evaluation
(ESR-F) of Example 1, a ground-band extract (ESR-E) of Comparative Example 1 and a skin astringent agent in the art to evaluate the skin elasticity improvement effect of so- Known tannic acid (TA) was mixed with a solution of hemoglobin (0.5%) in a ratio of 1: 1. Each of the mixtures was centrifuged at 3,000 rpm for 3 minutes and the absorbance was measured at 576 nm. The rate of decrease between the absorbance of each mixture thus obtained and the absorbance of the negative control group was calculated and shown in FIG.
As can be seen from Fig. 2, it was confirmed that the ground-band fermented product (ESR-F) had a skin-converging activity in a concentration-dependent manner. It was superior to tannic acid (TA), which is known to be a skin astringent in the art. Thus, it was found that ESR-F shrinks skin pores and improves skin elasticity I could. In addition, FIG. 2 shows that the groundnut fermented product as an active ingredient of the composition according to the present invention has significantly superior skin astringency than earthworm extract (ESR-E).
[ Experimental Example 3] Groundbreaker Fermented product ( ESR -F) melanin production Inhibition evaluation
The amount of melanin biosynthesis in melanocytes was measured by measuring the content of melanin in the cells. 1 × 10 5 B16F10 cells (Korean Cell Line Bank) were cultured in a cell culture dish for 24 hours, and α-MSH (α-Melanocyte-stimulating hormone, α-M), which produces melanin in melanocyte cells, To each cell to induce melanogenesis. The experimental group treated with only 100 nM of α-MSH (α-Melanocyte-stimulating hormone, α-M) was used as a negative control. 25 μg / ml of arbutin and 50 μg / ml of arbutin The experimental group treated with melanogenesis-induced cells and the group treated with melanogenesis-induced cells (25 μg / ml and 50 μg / ml, respectively) of groundnut extract (ESR-E) prepared in Comparative Example 1 were used as positive control. Melanogenesis-inducing cells were treated with 25 μg / ml and 50 μg / ml of each of the control groups and the ground-bed fermentation product (ESR-F) obtained in Example 1, and each of the experimental groups was treated with phosphate buffered saline ), Treated with 1 N NaOH solution containing 10% DMSO (dimethyl sulfoxide), and reacted at 80 ° C for 1 hour. Thereafter, the absorbance was measured at 475 nm to obtain Fig.
As can be seen from Fig. 3, the ground-bed fermentation product (ESR-F) was found to drastically reduce the melanin content produced by? -MSH. The effect of such a ground-up fermented product (ESR-F) is far superior to the case of treating arbutin (Arb), which is known to have an inhibitory effect on melanin production in the art. It has excellent whitening effect, And a whitening agent that is not cytotoxic. In addition, FIG. 3 confirms that the melanin production inhibitory effect of the ground-wing fermented product is much better than that of the ground-bed extract (ESR-E).
[ Experimental Example 4] Groundbreaker Fermented product ( ESR -F) Tyrosinase activation Inhibition And L- dopa Evaluation of oxidation inhibiting ability
0.2 mL of the substrate solution prepared by dissolving 10 mM L-dopa in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8), 0.1 mL of each test sample, and 0.2 mL of tyrosinase (110 U / mL) Followed by reaction at 25 ° C for 2 minutes. Each experimental sample contained arbutin (Arb), groundbone extract (ESR-E), which was known to have inhibitory activity of tyrosinase activity and L-dopa oxidation inhibition in the art, groundbreak extract (ESR-E) Each of the fermented products (ESR-F) was used at a concentration of 100 μg / ml, 200 μg / ml and 400 μg / ml. The absorbance of the reactant was confirmed at 475 nm, and the inhibition rate of dopa oxidation was calculated therefrom, as shown in FIG. 4 (b). The tyrosinase inhibition rate shown in FIG. 4 (a) was determined from the absorbance obtained in each experimental sample and the absorbance reduction rate in the experimental group to which no sample was added.
As a result, as shown in Fig. 4 (a), it was confirmed that the ground-band fermented product (ESR-F) inhibited the oxidation of tyrosinase and dopa related to melanin production in a concentration-dependent manner. In addition, it was confirmed that the effect of ESR-F was much better than that of ESR-E.
[ Experimental Example 5] Groundbreaker Fermented product ( ESR -F) MITF Expression Inhibition evaluation
B16F10 cells (Korean Cell Line Bank) were cultured in a 5% CO 2 incubator for 24 hours in a cell culture dish, and then α-MSH (α-Methylene-stimulating hormone, α-M ) 100 [mu] g / ml to each cell to induce melanogenesis. Then, each cell was treated with an experimental sample and incubated for 24 hours. RNA was extracted from each cell and the expression pattern of MITF gene related to melanogenesis was observed by RT-PCR. The ground band extract (ESR-E) prepared in Comparative Example 1 and the ground-band fermented product (ESR-F) obtained in Example 1 were treated at a concentration of 50 μg / ml and 100 μg / ml, respectively. The sequences of the primers used in the RT-PCR were as follows.
GAPDH F: GGT GCT GAG TAT GTC GTG GA (SEQ ID NO: 1)
GAPDH R: GAC ACA TTG GGG GTA GGA AC (SEQ ID NO: 2)
MITF F: AGG GGA AAA CTT GGT GTG AG (SEQ ID NO: 3)
MITF R: TAG AAA CGC TGG AAG GAA GG (SEQ ID NO: 4)
As a result, as shown in FIG. 5, it was confirmed that the ground-band fermented product (ESR-F) inhibits melanogenesis by inhibiting the expression of MITF related to melanin production. On the other hand, it was also confirmed that the effect of the ground-fed fermented product (ESR-F) was far superior to that of the ground-band extract (ESR-E).
[ Experimental Example 6] Groundbreaker Fermented product ( ESR -F) is cytotoxic
Mouse-derived B16 / F10 cells (Korean Cell Line Bank) and human-derived CCD-986sk cells (Korean Cell Line Bank) were used to confirm cytotoxicity. Experimental samples were treated with DMEM (Lonza) medium containing 1% penicillin / streptomycin (GIBCO) and 10% fetal bovine serum (GIBCO) 2 < / RTI > The ground-band fermented product prepared in Example 1 and the ground-band extract (ESR-E) prepared in Comparative Example 1 were used as test samples at concentrations of 100 μl / ml, 50 μl / ml, 25 μl / ml and 12.5 μl / . After that, cytotoxicity was confirmed using MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay in each cultured cell. MTT reacts with mitochondrial enzymes in living cells with a light yellow matrix to form Formazan. The formazan is dissolved in DMSO (Dimethyl sulfoxide), and the cell viability can be measured by measuring the absorbance at 570 nm.
As a result, as shown in FIG. 6, the groundnut fermented product as an active ingredient of the composition according to the present invention showed no cytotoxicity at the total experimental concentration, while the ground band extract (ESR -E) was found to have cytotoxicity as it was confirmed that the cell survival rate was rapidly lowered at some concentrations.
Hereinafter, a formulation example of the composition according to the present invention will be described, but it can be applied to various formulations, which are not intended to limit the present invention but to describe it specifically.
[Formulation Example 1] Soft capsule
The soft capsules were prepared by mixing 150 mg of fermented ground wax, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish oil, and 6 mg of lecithin and 400 mg per capsule according to a conventional method.
[Formulation Example 2] Tablets
The granules were prepared by mixing 150 mg of fermented ground wax, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate and 40 mg of 30% ethanol, followed by drying at 60 ° C. And the tablet was tableted.
[Formulation Example 3] Granules
The granules were formed by mixing 150 mg of fermented ground wax, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch, and adding 100 mg of 30% ethanol, followed by drying at 60 ° C to form granules, . The final weight of the contents was 1 g.
[Formulation Example 4] Drinking agent
150 mg of fermented ground wax, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled in a bottle. The final volume of the contents was adjusted to 200 ml.
[Formulation Example 5] Preparation of health food
Landfill fermented water .............................. 1000 mg
Vitamin mixture
Vitamin A Acetate ........................... 70 ㎍
Vitamin E ................................... 1.0 mg
Vitamin B1 .................................. 0.13 mg
Vitamin B2 ................................. 0.15 mg
Vitamin B6 ................................... 0.5 mg
Vitamin B12 .............................. 0.2 g
Vitamin C ..................................... 10 mg
Biotin ....................................... 10 μg
Nicotinic acid amide ............................ 1.7 mg
Folic acid ......................................... 50 ㎍
Calcium pantothenate ............................... 0.5 mg
Mineral mixture
Ferrous sulfate .................................. 1.75 mg
Zinc oxide ................................... 0.82 mg
Magnesium carbonate ............................... 25.3 mg
Potassium Phosphate ................................. 15 mg
Secondary calcium phosphate .............................. 55 mg
Potassium citrate ................................... 90 mg
Calcium carbonate .................................... 100 mg
Magnesium chloride 24.8 mg
Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.
[Formulation Example 6] Preparation of health drink
Landfill fermented water ............................... 1000 ㎎
Citric acid ..................................... 1000 mg
Oligosaccharides ...................................... 100 g
Plum concentrate ..................................... 2 g
Taurine .......................................... 1 g
Purified water was added to the mixture to complete 900 ml
The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >
Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, the demanding country, the use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
[Formulation Example 1] Lotion
[Formulation Example 2] Cream
[Formulation Example 3] Pack
[Formulation Example 4]
[Formulation Example 5] Ointment
[Formulation Example 6] ointment
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments.
<110> WORLD COSTEC. CO., LTD
<120> COMPOSITION FOR WHITENING CONTAINING FERMENTED SPOTTED SPURGE
<130> P15-0509
<160> 4
<170> KoPatentin 3.0
<210> 1
<211> 20
<212> RNA
<213> Artificial Sequence
<220>
<223> GAPDH foward primer
<400> 1
Claims (7)
The ground-bed fermented product was fed into the ground bed ( Euphorbia humifusa , Euphorbia maculata , and Euphorbia supina ). < / RTI >
Wherein the ground vinous fermentation product is a ground wastewater and a fermentation product of microorganisms.
Wherein the microorganism is at least one selected from the group consisting of fungi, yeast, and lactic acid bacteria.
Wherein the microorganism is Lactobacillus sakei .
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