KR20230060310A - Antibacterial and antioxidant composition comprising fermented Centella asiatica extract fermented by inoculating Bacillus safensis strain - Google Patents

Abstract

The present invention relates to an antibacterial and antioxidant composition containing a fermented extract of Centella asiatica by inoculating and fermenting a novel Bacillus safensis strain, and use thereof. The antibacterial and antioxidant composition contains a fermented extract of Centella asiatica by inoculating and fermenting a new strain, Bacillus safensis AMI-1407 with accession number KCCM13051P. Accordingly, as antibacterial and antioxidant effects against Propionibacterium acnes can be provided, the antibacterial and antioxidant composition is effective.

Description

Antibacterial and antioxidant composition comprising fermented Centella asiatica extract fermented by inoculating Bacillus safensis strain}

The present invention relates to an antibacterial and antioxidant composition comprising a fermented centella asiatica extract obtained by inoculating and fermenting a novel Bacillus sapensis strain or its use.

Skin is a membrane that covers the outside of the body and plays a role in protecting and regulating internal organs and other internal organs by performing various physiological functions that protect the body from various external stimuli, obstacles, and environmental factors such as dryness. do.

However, as the age of the skin increases, the physiological function of the body decreases, the secretion of various hormones that control metabolism decreases, and the function of immune cells and the activity of cells decrease. In addition, the appearance or function of the skin changes due to repeated exposure to light or heat. As a result, free radicals and active harmful oxygen increase, excessive physical and chemical stimulation and stress cause the normal function of the skin to deteriorate and melanin deposition to cause blemishes. , freckles, etc., and promote skin aging, thereby damaging the skin.

In order to prevent such skin aging and damage and maintain healthier and more beautiful skin, physiologically active substances obtained from various animals, plants, microorganisms, etc. are added to cosmetics and used to maintain the unique function of the skin and activate skin cells to promote skin growth Efforts are being made to maintain skin health by promoting metabolism, and accordingly, cosmetics, skin external agents, and the like are being developed.

However, when these materials are applied to the skin, safety problems such as irritation, erythema, redness, etc. limit the amount of use, or the effect is insignificant, so it is difficult to expect a substantial improvement in skin function. Therefore, there is a demand for the development of natural extracts that are safer to the body and have higher effects than existing compositions for external application for skin.

Korean Patent Application No. 10-2012-0148892 relates to a Bacillus sapensis strain having antibacterial activity. However, there is no disclosure of a novel Bacillus sapensis strain that exhibits antibacterial activity against acne-related strains or exhibits an antioxidant effect as well.

Accordingly, the present inventors have made diligent efforts to provide a natural extract capable of providing both antibacterial and antioxidant effects. Centella asiatica fermented with Bacillus sapensis AMI-1407, a novel Bacillus sapensis strain derived from forsythia. The present invention was completed by confirming that the fermented extract exhibits antibacterial and antioxidant effects against acne bacillus.

Accordingly, an object of the present invention is to provide an antibacterial and antioxidant composition comprising a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain or a use thereof.

The present invention provides an antibacterial and antioxidant composition comprising a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain.

According to a preferred embodiment of the present invention, the Bacillus safensis strain is Bacillus safensis AMI-1407 deposited under accession number KCCM13051P.

According to a preferred embodiment of the present invention, the centella asiatica fermented extract is extracted using water, an organic solvent, or a mixture thereof as a solvent.

According to a preferred embodiment of the present invention, the composition has antibacterial activity against Propionibacterium acnes bacteria.

In addition, the present invention provides a cosmetic composition for antibacterial and anti-oxidation containing a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain.

In addition, the present invention provides a quasi-drug composition for antibacterial and anti-oxidation containing a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain.

In addition, the present invention provides an antibacterial and antioxidant food composition comprising a fermented centella asiatica extract fermented by inoculating a Bacillus safensis strain.

In addition, the present invention provides an antibacterial and antioxidative companion product composition comprising a fermented centella asiatica fermented extract obtained by inoculating and fermenting a Bacillus safensis strain.

The present invention includes a fermented centella asiatica extract obtained by inoculating and fermenting Bacillus safensis AMI-1407 ( Bacillus safensis AMI-1407), a novel strain, accession number KCCM13051P, thereby antibacterial effect on Propionibacterium acnes bacteria And it can provide an antioxidant effect, so it is effective as an antibacterial and antioxidant composition.

1 is prepared by inoculating (0, 10%, 25%, 50%) Bacillus safensis AMI-1407 ( Bacillus safensis AMI-1407, B.safensis ) into centella asiatica and fermented fermented product by sterilization (hot water) extraction method The ABTS antioxidant efficacy evaluation results of the fermented centella asiatica extract are shown. It was confirmed that even if B. safensis was inoculated and fermented, the antioxidant effect was not affected.
Figure 2 is a fermentation product fermented by inoculating (0, 10%, 25%, 50%) Bacillus safensis AMI-1407 ( Bacillus safensis AMI-1407, B.safensis ) in Centella Asiatica, and 70% EtOH (stirring) extraction method Shows the ABTS antioxidant efficacy evaluation results of the extract prepared by. In the fermented Centella Asiatica inoculated with B.safensis at 25% level, the antioxidant effect increased by 6.3% compared to the control group, and it was confirmed that the efficacy was higher than that of the fermented Centella Asiatica inoculated with B.safensis at 10% and 50% level.
Figure 3 is a sterilization (hot water) extraction method or 70 fermented product fermented by inoculating (0, 10%, 25%, 50%) Bacillus safensis AMI-1407 ( Bacillus safensis AMI-1407, B.safensis ) in centella asiatica. The antibacterial efficacy evaluation results of the extract prepared by the % EtOH (stirring) extraction method are shown. When the fermented centella asiatica was extracted by sterilization extraction method, it did not show antibacterial activity against Propionibacterium acnes bacteria, but B.safensis was inoculated and fermented at 25%. When extracted, it was confirmed that it exhibits antibacterial activity against Propionibacterium acnes.

Hereinafter, the present invention will be described in more detail.

The 'fermented centella asiatica extract' of the present invention is prepared by preparing a centella asiatica extract and then fermenting it with Bacillus safensis AMI-1407 of accession number KCCM13051P, a novel strain derived from forsythia, to prepare a centella asiatica fermented product, It means an extract prepared by extracting it again.

The centella asiatica fermented extract fermented with Bacillus safensis AMI-1407 of accession number KCCM13051P, which is a novel strain derived from forsythia provided by the present invention, has an antibacterial effect against Propionibacterium acnes bacteria And it can provide an antioxidant effect, so it can be effectively used as an antibacterial and antioxidant composition.

Specifically, as a result of confirming the ABTS+ radical scavenging activity, when extracting the fermented centella asiatica fermented product by inoculation with Bacillus safensis AMI-1407 (Accession No. KCCM13051P) by sterilization (hot water) extraction method, antibacterial, antioxidant to It did not show an anti-aging effect. On the other hand, when the centella asiatica fermented product was extracted with 70% EtOH (stirring) extraction method, it was confirmed that anti-aging activity through anti-oxidation and antibacterial activity against Propionibacterium acnes were exhibited. In particular, in the case of antibacterial activity, it was not increased by inoculating a lot of B. safensis bacteria during fermentation of the Centella asiatica extract, and the best antibacterial activity was exhibited when inoculated at 15% to 35% of the weight of the Centella asiatica extract (FIG. 3).

Accordingly, the present invention can provide an antibacterial and antioxidant composition comprising a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain.

According to a preferred embodiment of the present invention, the Bacillus safensis strain may be Bacillus safensis AMI-1407 of accession number KCCM13051P.

According to a preferred embodiment of the present invention, the Bacillus safensis strain may be derived from Forsythia.

According to a preferred embodiment of the present invention, the centella asiatica fermented extract may be extracted using water, an organic solvent, or a mixture thereof as a solvent. Preferably, the centella asiatica fermented extract is preferably extracted using water, C1 to C4 lower alcohol, or a mixture thereof as a solvent, and more preferably extracted using water, methanol, ethanol, propanol, or a mixture thereof as a solvent. It is preferred, and most preferably, ethanol is used as a solvent. The ethanol may be 1% to 100% ethanol.

According to a preferred embodiment of the present invention, the centella asiatica fermented extract is at least one method selected from the group consisting of reduced pressure and high temperature extraction, hot water extraction, reflux extraction, stirring extraction, hot water extraction, room temperature extraction, ultrasonic extraction and steam extraction. It may be extracted with, preferably it may be prepared by a stirring extraction method.

After the extraction process, a purification process to increase the purity of the extract, such as filtration, centrifugation or concentration process, may be additionally included. The concentration may be performed by a method such as precipitation concentration, evaporation concentration, vacuum concentration, ultrafiltration, reverse osmosis, or centrifugation, but is not limited thereto.

According to a preferred embodiment of the present invention, the composition may have antibacterial activity against Propionibacterium acnes bacteria. The antibacterial activity may be exhibited when fermenting the centella asiatica extract by inoculating B. safensis bacteria in an amount greater than 10% to less than 50% compared to the centella asiatica extract. Preferably, it may appear when inoculated and fermented at 15% to 35% by weight of centella asiatica extract, more preferably by inoculating B. safensis bacteria at 20% to 30% by weight of centella asiatica extract.

In addition, the present invention can provide a cosmetic composition for antibacterial and anti-oxidation containing a fermented centella asiatica extract fermented by inoculating a Bacillus safensis strain.

Since the Centella asiatica fermented extract to antibacterial is the same as the concept used in the antibacterial and antioxidant composition, the description is replaced with the description.

The cosmetic composition of the present invention contains the centella asiatica fermented extract as an active ingredient and a basic cosmetic composition (toilet, skin protectant, cream, essence, face wash such as cleansing foam and cleansing water, pack, body oil) together with dermatologically acceptable excipients , body cleanser), color cosmetic composition (foundation, lipstick, mascara, makeup base), hair product composition (shampoo, rinse, scalp cleanser, hair conditioner, hair gel) and soap.

The excipient is not limited thereto, but may include, for example, a skin softener, a skin penetration enhancer, a colorant, a fragrance, an emulsifier, a thickening agent, and a solvent. In addition, it may additionally include flavoring agents, pigments, bactericides, antioxidants, preservatives, moisturizing agents, and the like, and thickeners, inorganic salts, synthetic polymer materials, and the like may be included for the purpose of improving physical properties. For example, when preparing a facial cleanser and soap with the cosmetic composition of the present invention, it can be easily prepared by adding the centella asiatica fermented extract to a conventional facial cleanser and soap base. When preparing a cream, it can be prepared by adding the centella asiatica fermented extract to a general oil-in-water (O/W) cream base. Herein, synthetic or natural materials such as flavors, chelating agents, pigments, antioxidants, preservatives, and proteins, minerals, and vitamins for the purpose of improving physical properties may be additionally added.

The content of the fermented centella asiatica extract contained in the cosmetic composition of the present invention is not limited thereto, but is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight, based on the total weight of the total composition. If the content is less than 0.001% by weight, the desired antibacterial and antioxidant effects cannot be expected, and if it exceeds 10% by weight, there may be difficulties in safety or preparation of the formulation.

In addition, the present invention can provide a quasi-drug composition for antibacterial and anti-oxidation containing a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain.

Since the Centella asiatica fermented extract to antibacterial is the same as the concept used in the antibacterial and antioxidant composition, the description is replaced with the description.

The term 'quasi-drugs' of the present invention refers to items that have a milder effect than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, mitigating, treating or preventing diseases of humans or animals. For example, according to the Pharmaceutical Affairs Act, quasi-drugs Products used for the purpose of medicine are excluded, and include products used for the treatment or prevention of diseases in humans and animals, and products with mild or no direct action on the human body.

When the fermented centella asiatica extract of the present invention is used as a quasi-drug additive, it may be added as it is or used together with other quasi-drug ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient can be suitably determined depending on the purpose of use (prevention, health or therapeutic treatment).

Preferably, the quasi-drug composition may be a disinfectant cleanser, shower foam, wet tissue, detergent soap, hand wash, mask or ointment, but is not limited thereto.

In addition, the present invention can provide an antibacterial and antioxidant food composition comprising a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain.

Since the Centella asiatica fermented extract to antibacterial is the same as the concept used in the antibacterial and antioxidant composition, the description is replaced with the description.

The food composition according to the present invention can be prepared in various forms according to conventional methods known in the art. General foods include, but are not limited to, beverages (including alcoholic beverages), fruits and their processed foods (e.g. canned fruits, bottled products, jams, marmalades, etc.), fish, meat and their processed foods (e.g. ham, sausages) Corned beef, etc.), breads and noodles (e.g. udon, buckwheat noodles, ramen, spagate, macaroni, etc.), fruit juice, various drinks, cookies, taffy, dairy products (e.g. butter, cheese, etc.), edible vegetable oil, margarine , Vegetable protein, retort food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.) can be prepared by adding the centella asiatica fermented extract of the present invention. In addition, nutritional supplements are not limited thereto, but may be prepared by adding the centella asiatica fermented extract of the present invention to capsules, tablets, pills, etc. In addition, the fermented centella asiatica extract itself of the present invention can be prepared in the form of tea, juice, and drink, and can be ingested by liquefying, granulating, encapsulating, and powdering so that it can be consumed (healthy beverage). In addition, in order to use the fermented centella asiatica extract of the present invention in the form of a food additive, it can be prepared and used in the form of a powder or concentrate. In addition, it can be prepared by mixing the fermented centella asiatica extract of the present invention with a known active ingredient known to have antibacterial and antioxidant effects.

When using the fermented centella asiatica extract of the present invention as a health drink, the health drink composition may contain various flavoring agents or natural carbohydrates as additional components like conventional drinks. The aforementioned natural carbohydrates include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrins and cyclodextrins; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol. Sweeteners include natural sweeteners such as thaumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition, the centella asiatica fermented extract of the present invention may be contained as an active ingredient in the antibacterial and antioxidant health functional food composition, the amount of which is not particularly limited to an effective amount to achieve antibacterial and antioxidant activity, based on the total weight of the total composition It is preferably from 0.01 to 100% by weight relative to. The health functional food composition of the present invention may be prepared by mixing the centella asiatica fermented extract with other active ingredients known to have antibacterial and antioxidant effects.

In addition to the above, the health functional food of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloidal thickeners, pH regulators, stabilizers, and preservatives. , glycerin, alcohol or carbonation agent, and the like. In addition, the health functional food of the present invention may contain fruit flesh for the production of natural fruit juice, fruit juice beverage, or vegetable beverage. These components may be used independently or in combination. The ratio of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In addition, the present invention can provide an antibacterial and antioxidant companion product composition comprising a fermented centella asiatica extract obtained by inoculating and fermenting a Bacillus safensis strain.

Since the Centella asiatica fermented extract to antibacterial is the same as the concept used in the antibacterial and antioxidant composition, the description is replaced with the description.

The companion product of the present invention includes all necessary products for companion animals, but may preferably mean a cosmetic composition, a quasi-drug composition, a food composition, or a health functional food composition for companion animals. Description of the cosmetic composition, quasi-drug composition, food composition or health functional food composition is replaced with the above description.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for exemplifying the present invention, and it is obvious to those skilled in the art that the scope of the present invention is not construed as being limited by these examples.

Isolation of useful microorganisms

We tried to isolate and identify a new strain of Bacillus sapensis from wild forsythia in Jeju Island.

Specifically, wild forsythia from Jeju Island was suspended in sterilized physiological saline and smeared on each corresponding selective medium. Culture was carried out under anaerobic conditions at 30-37 ° C. for 24 to 48 hours, and strains with different colony types were selected and purified through several pure culture processes. The pure isolated strain was plated on a general bacterial culture solid medium (Plate count agar) and cultured at 35 ° C for 24 hours. Only the cells were obtained, mixed with 20% (v / v) glycerol, and stored in a -70 ° C cryogenic freezer.

The isolated strain was identified through 16S rDNA sequence analysis (Bacillus sapensis AMI-1407, Bacillus safensis AMI-1407), and the base sequence was confirmed by requesting Solgent Co., Ltd. Primers used for strain isolation were universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG -3', SEQ ID NO: 1) and 1492R (5'-GGTTACCTTGTTACGACTT-3', SEQ ID NO: 2).

Production of fermented centella asiatica

After washing with purified water and drying the centella asiatica plant, it was coarsely pulverized and mixed with twice as much purified water, followed by high-pressure hot water extraction at 121 ° C. for 15 minutes. Bacillus safensis AMI-1407 for fermentation ( Bacillus safensis AMI-1407) was used for sterilized general bacterial culture medium (Plate count broth, Difco, United States of America) or natural product medium, and after culture was completed, 1 × 10 ⁷ cfu / It was inoculated with centella asiatica extract at a concentration of ㎖. Fermentation was carried out using a solid fermenter for 3 to 10 days, maintaining 30 ° C. and humidity. Fermented centella asiatica fermented product was dried at 40 ℃ for 12 to 48 hours using a hot air dryer.

Preparation of Centella asiatica fermented extract

<3-1> Preparation of Centella asiatica fermented hot water extract

The fermented centella asiatica prepared in <Example 1> was mixed with 20 times lava seawater and mineral water, followed by high-pressure hot water extraction at 121 ° C. for 15 minutes.

<3-2> Preparation of Centella asiatica fermented ethanol extract

After mixing with 20 times 70% ethanol of the dried centella asiatica prepared in <Example 1>, a magnetic bar was inserted and the rotation speed was set to about 300 rpm on a stirrer, and stirring and extraction were performed for 3 to 7 days.

Confirmation of antioxidant activity - ABTS ⁺ Confirmation of radical scavenging activity

ABTS ⁺ [2,2'-Azino-bis(3 ^- ethylbenzothiazoline-6-sulfonic acid)] radical scavenging activity is a measurement method that can measure both hydrogen-donating antioxidants and chainbreaking antioxidants and is applicable to both aqueous phase and organic phase. ⁺ It is a colorimetry method using the principle that when a solution is reduced by an antioxidant, it becomes colorless.

Specifically, a 7 mM ABTS solution was mixed with potassium persulfate to a concentration of 2.45 mM, and then reacted for about 16 hours in the dark to prepare a blue-green ABTS ⁺ solution diluted with distilled water. Samples were diluted 1/2-fold from the highest concentration of 1000 μg/ml, and 50 μl of each of the 7 concentrations and the solvent (untreated group) was added to a 96-well plate, and 50 μl of each prepared ABTS ⁺ solution was added, and then in the dark After reacting for 20 minutes, absorbance was measured at 700 nm using a microplate reader. The resulting value was expressed as a percentage (%) of radical scavenging activity through calculation using the following [Equation 1] by comparing the treatment group and the untreated group of the extract sample prepared in <Example 3>.

[Equation 1]

ABTS ⁺ scavenging activity (%) = [1 - (absorbance of Example or Comparative Example sample treated group / absorbance of sample untreated group)] X 100 (%)

As a result, the fermented centella asiatica extract prepared by the sterilization (hot water) extraction method did not affect the antioxidant effect even if the B. safensis inoculation amount was increased (FIG. 1). On the other hand, the fermented centella asiatica extract prepared by the 70% EtOH (stirring) extraction method confirmed that the antioxidant effect increased by 6.3% and 5.0%, respectively, compared to the control group at a concentration of 1000 μg/ml when the inoculation amount of B.safensis was 25% or 50%. could (Fig. 2).

ABTS ⁺ radical scavenging activity (%) 0% fermentation
hot water extract 10% fermentation
hot water extract 25% fermentation
hot water extract 50% fermentation
hot water extract 86.5 73.8 76.7 74.4 0% fermented 70% ethanol extract 10% fermentation
70% ethanol extract 25% fermentation
70% ethanol extract 50% fermentation
70% ethanol extract 92.4 81.1 98.7 97.4

Confirmation of antibacterial activity

In order to measure the antibacterial efficacy of the fermented centella asiatica extract of the present invention against acne-causing skin fungi, analysis was performed using a paper disc diffusion assay method, which is a method for confirming growth arrest.

Propionibacterium acnes (ATCC 6919) was cultured in RCM liquid medium for 48 hours, and then diluted to a level of 1.0×10 ⁶ cfu/ml in sterile saline (0.9% NaCl). After smearing 100 μl of the diluted bacterial culture on RCM solid medium (Agar 1.5%) (final level of 1.0 × 10 ⁵ cfu/ml), make the convex part of the paper disc face up with sterilized forceps, % inoculation and 50% inoculation) and centella asiatica extract, positive control erythromycin 0.01mg/ml and negative control were injected with 40 μl each, and left for a while so that the paper disc would not fall off, turned over and cultured for 48 hours under anaerobic conditions to prevent inhibition Antibacterial activity was confirmed by checking whether or not it was produced.

As a result, as shown in [Fig. 3], the centella asiatica fermented extract prepared by the sterilization (hot water) extraction method did not show an antibacterial effect even if the B. safensis inoculation amount was increased. On the other hand, it was confirmed that the fermented centella asiatica extract prepared by the 70% EtOH (stirring) extraction method had an increased antioxidant effect compared to the control at a concentration of 100 mg/ml when the B.safensis inoculum was 25% (control: 13 mm, 25% : 17mm).

Low ring (mm) hot water extract
0% fermentation
70% ethanol extract 10% fermented 70% ethanol extract 25% fermented 70% ethanol extract 50% fermented 70% ethanol extract Positive control (erythromycin) voice control phrase
(DW) voice control phrase
(EtOH) voice control phrase
(EtOH:DMSO=1:1) 0 13 13 17 15 35 0 0 0

Korea Microbial Conservation Center (Overseas) KCCM13051P 20210928

SEQUENCE LISTING <110> AMI COSMETIC CO., LTD. <120> Antibacterial and antioxidant composition comprising fermented Centella asiatica extract fermented by inoculating Bacillus safensis strain <130> 1069920 <160> 2 <170> PatentIn version 3.2 <210> 1 <211> 20 <212> DNA <213> artificial <220> <223> Universal primer_27F <400> 1 agagtttgat cctggctcag 20 <210> 2 <211> 19 <212> DNA <213> artificial <220> <223> Universal primer_1492R <400> 2 ggttaccttg ttacgactt 19

Claims (8)

Bacillus sapensis ( Bacillus safensis ) Antibacterial and antioxidant composition comprising a fermented centella asiatica extract fermented by inoculating the strain.
The antibacterial and antioxidant composition according to claim 1, wherein the centella asiatica fermented extract is extracted using water, an organic solvent, or a mixture thereof as a solvent.
According to claim 1, wherein the composition Propionibacterium acnes ( Propionibacterium acnes ) Antibacterial and antioxidant composition characterized in that it has an antibacterial activity against the bacteria.
According to claim 1, wherein the Bacillus sapensis ( Bacillus safensis ) The strain deposited as Bacillus sapensis AMI-1407 accession number KCCM13051P ( Bacillus safensis AMI-1407) characterized in that the antibacterial and antioxidant composition.
Bacillus sapensis ( Bacillus safensis ) Antibacterial and antioxidant cosmetic composition comprising a fermented centella asiatica extract fermented by inoculating the strain.
Bacillus sapensis ( Bacillus safensis ) Antibacterial and antioxidant quasi-drug composition comprising a fermented centella asiatica extract obtained by inoculation and fermentation.
Bacillus sapensis ( Bacillus safensis ) Antibacterial and antioxidant food composition comprising a fermented centella asiatica extract fermented by inoculating the strain.
Bacillus sapensis ( Bacillus safensis ) Antibacterial and antioxidative companion product composition comprising a fermented centella asiatica extract fermented by inoculating the strain.

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