KR20110120813A - Cosmetic composition comprising euphorbia supina for improving skin wrinkle - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Euphorbia supinaextract for anti-wrinkling is provided to suppress collagenase activity and promote collagen generation without side effect. CONSTITUTION: A cosmetic composition for anti-wrinkling contains Euphorbia supinea extract as an active ingredient. The extract is isolated using organic solvent such as C1-C4 low alcohol, aromatic hydrocarbon solvent or mixture thereof, ethanol, methanol, methylene chloride, chloroform, hexane, heptane, benzene, toluenen, or mixture thereof. A method for preparing the extract comprises: a step of pretreating Euphorbia supinea with the organic solvent; and a step of drying residue and isolating with C1-C4 low alcohol. The cosmetic composition contains isoquercitrin as an active ingredient, isolated from the extract. The cosmetic composition is manufactured in the form of a lotion, nutrition lotion, massage cream, cleansing product, body products, ointment, gel, cream, patch, or spray.

Description

Cosmetic composition for improving wrinkles comprising silk extract {Cosmetic composition comprises Euphorbia supina for improving skin wrinkle}

The present invention is silk ( Europhorbia) The present invention relates to a cosmetic composition for improving wrinkles comprising the extract as an active ingredient and a method for preparing the same, and more specifically, to inhibiting collagenase activity of silk grass extracts for wrinkle improvement using a collagen production promoting effect. It relates to a cosmetic composition.

Euphorbia supina is an annual herb that accompanies the Great Family , and is a wild weed widely distributed in temperate and tropical regions such as Korea, China, Japan, Southeast Asia, and North and South America. It is similar to purslane, but much smaller than purslane, grass or yard, It grows on the road floor, but it is small so it is not noticeable. The stem grows while the ground grows, and the white juice comes out quite a lot when the stem or leaf is cut. When many branches are split from the base to cover the ground, there are some hairs on the stem, and the branches are usually divided into two pieces and turn red (see Fig. 1).

With the aging society, interest in skin aging is rapidly increasing, interest in skin wrinkles is increasing day by day, and the market for wrinkle improvement cosmetics is expanding rapidly with the implementation of the Functional Cosmetics Act in 2000. Many researches have been conducted on cosmetic materials.

An object of the present invention is to provide a cosmetic composition using a natural material excellent in wrinkle improvement without skin toxicity.

Euphorbia supina extract in the study of the present inventors to achieve the above object has no skin toxicity and excellent antioxidant (DPPH, ORAC) effect, in particular collagenase (Collagenase) inhibitory activity and collagen (Collagen) production promoting effect Do. In particular, isoquercitrin obtained by separating and purifying the solvent fraction of silkweed extract has no skin toxicity, and has excellent antioxidant effect, collagenase activity inhibition and collagen (Collagen) production promoting effect. The present invention was found to be able to produce a cosmetic for improving wrinkles having excellent anti-wrinkle functionality.

The present invention is silk ( Europhorbia) By providing a cosmetic composition for improving wrinkles and a method for preparing the same, comprising a supina ) extract as an active ingredient, it is possible to provide a cosmetic composition for improving wrinkles having excellent collagenase activity inhibition and collagen production promoting effects. . In particular, the isoquercitrin component isolated from Euphorbia supina has an excellent effect of inhibiting collagenase activity and promoting collagen production, and thus the present invention is an anti-wrinkle agent comprising isoquarcitrin isolated from silkweed as an active ingredient. Cosmetic compositions can be provided.

The present invention is silk ( Europhorbia) It can be used in cosmetics for wrinkle improvement in various formulations by utilizing collagenase activity inhibition and collagen production inhibitory function of isoquarcitrin isolated from extracts of supina ) and silk grass extracts. Extracts from natural substances or ingredients extracted from natural substances By containing it as an active ingredient, it is environmentally friendly and has little side effect.

1 is a photograph of Euphorbia supina .
Figure 2 shows the first extraction process of the silk ( Europhorbia supina ).
Figure 3 is silk ( Europhorbia) supina ) shows the solvent fraction flow chart of the primary extract.
Figure 4 shows the process up to the separation of isoquercitrin from Euphorbia supina .
Figure 5 shows the ESI-MS (positive) spectrum of isoquercitrin isolated from Euphorbia supina .
Figure 6 shows the ESI-MS (negative) spectrum of isoquercitrin isolated from Euphorbia supina .
7 is a 1 H NMR spectrum result of isoquercitrin isolated from Euphorbia supina .
8 is a chemical structural formula of Isoquercitrin isolated from Euphorbia supina .
9 is a gelatin egoography band graph in MMP-9 analysis.
11 is a graph showing the expression of MMP-9 in hairless mice treated in MMP-9 assay.
FIG. 11 is a graph showing collagen levels of hairless mice treated in the Collagen assay.
12 is a photograph showing a method of testing a human scalp on the inner side of the upper arm of the subject.
Figure 13 is a graph showing the results of the stability observation of the lotion prepared in the present embodiment.
14 is a graph showing the results of observation of the stability of the cream prepared in the present embodiment.
15 and 16 are process charts showing a process of preservative treatment after mixing, extracting and concentrating silk grass with a solvent suitable for application to cosmetics in accordance with an embodiment of the present invention, Figure 15 is a process chart before the preservative treatment, Figure 16 Is a process chart at the time of antiseptic process.

Hereinafter, the present invention will be described in detail.

The present invention is silk ( Europhorbia) supina ) provides a cosmetic composition for improving wrinkles containing the extract as an active ingredient. The cosmetic composition for wrinkle improvement of the present invention is silk ( Euphorbia) supina ) extract as an active ingredient, in particular the silk grass extract has collagenase activity inhibitory and collagen production promoting effect.

Euphorbia supina) extract is a solvent selected to characterized in that the organic solvent extract, the organic solvent is from the group consisting of C _{1 -4} lower alcohol, chlorinated hydrocarbon solvents, hydrocarbon solvents, aromatic hydrocarbon solvents, mixed solvents of the foregoing and Can be used.

In one embodiment of the present invention, the organic solvent may be ethanol, methanol, methylene chloride, chloroform, hexane, heptane, benzene, toluene or a mixed solvent thereof.

The invention also relates to Euphorbia supina ) provides a cosmetic composition for improving wrinkles containing isoquercitrin isolated from the extract as an active ingredient. The isoquarcitrin is excellent in inhibiting collagenase activity and promoting collagen production. Actually, silk grass is extracted with EtOH 70% to screen the efficacy firstly and accordingly liquid seperation of the material to verify the efficacy again, and subfraction to confirm the material as its effect and indicator component. Results Isoquercitrin was identified as an active ingredient and indicator component in silk grass.

In one aspect of the present invention, a cosmetic comprising the cosmetic composition is provided.

The cosmetics are not limited thereto, but for example, cosmetics, lotions, emulsions, nourishing lotions, nourishing creams, massage lotions, face washes, cosmetics, packs, body products, emulsified makeup, ointments, gels, creams, patches or sprays. Can be commercialized.

In one aspect of the present invention, the cosmetic composition for improving wrinkles of the present invention may be prepared in any formulation commonly prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, and the like. It is not limited to this. More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The present invention,

(a) pretreatment of Euphorbia supina with an organic solvent; And

and (b) drying the residue and provides a process for the preparation of bidanpul extract for improving wrinkles, comprising the step of extracting by using a C _{1 -4} lower alcohol solvent.

In the production method of the present invention, a hydrocarbon solvent may be used as the organic solvent of step (a), ethanol or methanol may be used as the solvent of step (b), and more specifically, hexane may be used as the organic solvent of step (a). As the solvent of step (b), ethanol may be used.

For example, the pretreatment with hexane may be carried out by removing the oil and wax layers with non-polar substances, extracting them with ethanol, fully concentrating, and converting the concentrate into a cosmetic solvent to facilitate the grafting of the concentrate to the cosmetics when the active ingredient is present in the ethyl acetate layer. Although it is preferable, the kind of pretreatment solvent may be appropriately selected depending on the kind of the extraction solvent and the kind of the fractionation solvent. The primary extract prepared as described above can be used to produce a cosmetic of the present invention.

In the production method of the present invention, after step (b) may further comprise the step of (c) fractionating with a solvent.

The solvent used in step (c) may include hexane, methylene chloride, methyl acetate, ethyl acetate, purified water, methanol, ethanol, butanol, 1,3-butylene glycol, propylene glycol, glycerin or benzene, and ethyl Acetate is preferred. In addition, in the case of solvent fractionation, cost, organic solvent residue, etc. may be a problem, so it is preferable to pretreat with hexane.

Hereinafter, the present invention will be described by the following examples and experimental examples. However, these are provided only to more easily understand the present invention, but the scope of the present invention is not limited thereto.

[ Example 1 ]

According to the process as shown in FIG. 2, after first extracting silk grass with 70% ethanol, hexane (Hexane), methylene chloride, ethyl acetate, butanol (Butanol) according to the process shown in FIG. The solvents such as) were sequentially processed to measure the activity of each fraction, thereby verifying the fraction in which the active ingredient is present and confirming the components present in the fraction. The initial yield of the primary extract is 28.2% and the yield of the solvent fraction compared to the initial extract is shown in FIG.

In order to separate and confirm the components, the principal fractions were performed according to the polarity of the solvent, and the yields were as follows. ) Was performed.

Isoquercitrin was confirmed by ESI-MS, 1 H NMR analysis of the separated material. FIG. 5 shows ESI-MS (positive) spectrum {ESI-MS (positive) m / z: 487 [M + Na]} of silk grass-derived isoquercitrin, and FIG. 6 shows silk grass-derived isoquercitrin (Isoquercitrin). ESI-MS (negative) spectrum {ESI-MS (negative) m / z: 463 [MH]} is shown, Figure 7 is a 1H NMR spectrum of Isoquercitrin isolated from silk grass (DMSO-d6, 400 MHz).

¹ H-NMR (DMSO-d ₆ , 400 MHz, δ ppm): 3.05 to 3.60 (sugar H), 5.45 (1H, d, J = 7.4 Hz, H-1 "), 6.19 (1H, d, J = 1.9 Hz, H-6), 6.39 (1H, d, J = 1.9 Hz, H-8), 6.83 (1H, d, J = 9.0 Hz, H-5 '), 7.57 (2H, m, H-2' , 6 '), 12.63 (1H, brs, 5-OH)

Figure 8 shows the structure of isoquercitrin (Isoquercitrin) separated from the silk grass based on this.

Experimental Example  1. Cell proliferation and cytotoxicity experiment MTT assay )

Cell growth curves were determined by 3- (4,5-dimethylthiazol-2-yl) -2,5-dibenzyltetrozolium (MTT; sigma, USA) analysis.

1) Cells (Mouse NIH / 3T3 fibroblasts) 2 × 10 ³ cells / well in DMEM 96-well plates containing 10% FBS (Fetal bovine serum) at 5% CO ₂ , 37 ℃ After 48 hours of incubation, the extracted sample was directly treated. MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-dibenzyltetrozolium; sigma, USA) (5 mg / ml) was administered at 10 μg / well and incubated for 4 hours. 100 μl of DMSO was added to each well and the supernatant was removed. After incubation for 10 minutes, the absorbance was measured at 550 nm using a micro-plate reader (SPCTRA MAX 340PC, Molecular Devices, USA). The absorbance is the number of living cells and the reproducibility was confirmed by three experiments. .

Cell growth rate (%) = [OD _{550 ( sample )} -OD _{550 ( control )} ] / OD _{550 ( control )} × 100

As a result of evaluating the cell proliferation effect and cytotoxicity for silk extract, solvent fraction, and each isolated material, it was confirmed that the cell proliferation effect was excellent and the cytotoxicity was very insignificant. (See Table 1).

Experimental Example  2. Antioxidant Experiment

Free radical  Scavenging activity analysis DPPH assay )

The free radical scavenging activity assay is an improvement on the method of Funita et al, which provides stable 1,1-diphenyl-2-picrylhydrazyl radicals (1,1-Diphenyl-2-picrylhydrazyl Radical: DPPH). Used (bleached by proton-radical scavenger). Rhubarb and silk grass mixed extracts were added to the DPPH solution, mixed, and then the absorbance was measured at 515 nm after being left at room temperature for a while.

Oxygen radical Absorption  analysis( Oxygen radical absorbance capacity asssay : ORAC  assay)

It is an experiment aimed at screening substances with antioxidant power as plant extracts. The reaction is started by adding a buffer, a sample, and a FL solution, and then adding a radical generator AAPH. The principle is FL decay caused by AAPH. The presence of antioxidants inhibits the FL decay response. FL intensity (intensity) was measured every 60 minutes for 60 minutes, and proceeded at 37 ℃, pH7.4 conditions.

materials( Material )

-Extract

Detection dye: Fluorescein disodium (FL)

2-2′-azobis (2-amidino-propane) dihydrochloride (AAPH)

96 well black plate

Trolox (water soluble analogue of Vie E, positive Control)

-Fluorostar Galaxy plate reader or Model F-4500 (HITACHI, Japan)-Can be replaced with a regular fluorometer and can be used with Vmax assay.

protocol( Protocol )

1. Fluoremeter and water bath power (37 ℃)

2. B-PE, phosphate buffer, samples were 15 minutes pre-incubation

3. Reaction volume = 200ul

75 mM phosphate buffer (pH7.4)

Control: Trolox (final 0.5 ~ 10ug / ml)

B-PE: FL (final 10uM)

Sample: final 0.5 ~ 10ug / ml

Radial generator: AAPH (final 50mM)

Allow AAPH to be added last. Each sample was run at least three replicates.

As a result of the evaluation, the antioxidative effect of the silkweed extract, the solvent fraction, and each of the separated substances was evaluated. (See Figures 4, Tables 2, 3, and 4)

Experimental Example 3. Collagenase  Inhibitory activity and collagen production promoting experiment

Collagenase  Method ( Collagenase assay )

It is evaluated using Actinomycetes-derived collagenase and synthetic substrate PZ-Peptide. As a buffer, 0.1 M Tris (hydrochloric acid) buffer (pH 7.1, containing 20 mM CaCl ₂ ) was used. The enzyme was prepared at 0.1 mg / ml in buffer and the substrate was prepared at 0.5 mg / ml in buffer. The sample was mixed with 50 µl, 50 µl of enzyme and 400 µl of substrate and reacted at 37 ° C. for 30 minutes, and then 1 ml of 25 mM citric acid solution was added to stop the reaction. Next, 5 ml of ethyl acetate was added and stirred, and the ethyl acetate layer was extracted.

After centrifugation (3000 rpm, 5 min), the absorbance was measured at a wavelength of 320 nm and the inhibition rate was calculated according to the following formula. The control was used as the solvent of the sample, the substrate was added after the reaction was stopped, and the blank was used.

As a result of evaluating the collagenase activity inhibitory effect on the extracts, the solvent fractions, and each of the separated substances, the collagenase activity inhibitory effect of the extracts of silkweed extract and the respective solvent fractions, in particular, isoquercitrin Was confirmed to be excellent (see Tables 5 and 6).

Collagen production promoting effect

1) MMP-9 assay

MMP-9, also called gelatinase B or 92 kDa gelatinase, is a gelatin, collagen type IV and type V of skin cytoplasm. Degradation activity for is known. Therefore, in the case of antiwrinkle functional cosmetic raw materials, the greater the inhibition of MMP-9 activity, the greater the protective effect against wrinkle formation, and it is possible to improve the elasticity of the skin by maintaining gelatin and collagen. have.

Zymography ( Zymography )

Sample: After inducing MMPs activity through UV irradiation in hairless mice, silk grass EtOH 70% extract concentrate was applied for 4 weeks and skin tissues were used for the test.

Each sample was separated by electrophoresis using a 10% polyacrylamide gel containing 0.1% gelatin

Washing the gel with a regeneration buffer containing 25% Triton X-100 for 30 minutes at room temperature

After incubation for 30 minutes incubation for 15 hours or more in 37 ℃ development buffer (buffing buffer)

2% brilliant blue, 50% ethanol, 10%

Staining for 1 hour in acetic sol.

Destaining for 30 minutes in methanol acetate / acetic sol (30: 10%).

-Fixed to DW.

Band density measurement using an imaging system.

2) Collagen Assay

Samples: After inducing MMPs activity through UV radiation to hairless rats, silk grass EtOH 70% extract concentrate was applied for 4 weeks and skin tissue was collected and used for the test.

Retinol (Roche) 25000 IU was used as a positive control.

Collagen content determination using a collagen assay kit

Incubating the same amount of protein lysate with collagen dye reagent

The reacted precipitate was dissolved in 0.5 M NaOH and the OD value was measured at 540 nm using a microplate reader.

The analysis results are presented in the graphs of FIGS. 9, 10 and 11 (Expression of MMP-9 in the hairless mouse treated with vehicle.control and test chemical group).

Experimental Example  4. Silk grass  Irritation test of extract

It was tested for skin irritation and alleviation of skin irritant and skin irritation through a simple clinical trial.

1) Skin irritation test of silk extract

- Test Methods

The evaluation of skin reactions was carried out according to the evaluation criteria of skin reactions indicated in Food and Drug Administration Notification No. 1999-61, "Toxicity Test Standards for Drugs, etc.". In addition, the determination of the degree of stimulus for the results was based on a method of calculating the primary irritation index (PII) of the commonly used dry (Draize).

Test result

In order to investigate the skin irritation of silkworm extract, EtOH 70% extract concentrate was dissolved 10% in purified water to prepare a test substance, and 0.5 ml of test substance was applied to 6 New Zealand White male rabbits. Each was applied for 24 hours to the pallor and non-chalcissus areas. Mortality, general symptoms, body weight change and local irritation were evaluated during the 72-hour observation period. the results are as follow.

1) No dead animals were observed due to application of test substance during the test period.

2) Abnormal findings due to the application of the test substance were not recognized.

3) As a result of weight measurement, no weight change due to application of the test substance was observed.

4) As a result of observing abrasion and non-brain and local irritation after finishing application of test substance, no abnormal findings by application of test substance were recognized. Therefore, the primary irritation index of this test substance was "0" and was evaluated as a non-irritant substance.

The above results suggest that the skin application of silk extract in New Zealand White rabbits does not cause any irritation in the abrasion and non-abrasive areas. Therefore, this test substance is considered to be non-irritant.

2) Simplified Clinical Trial of Silk Grass Extract

-Stimulation-relieving liver clinical trials were conducted using silk grass extract. As a result, it was confirmed that it has a stimulating effect and confirmed the possibility of developing cosmetics for sensitive skin in the future cosmetic prescription.

Silkweed extract concentrated powder was dissolved in purified water, and the stimulation relaxation effect using SLS (1.0%) human patch test was measured as follows for 0.1%, 0.5%, 1%, and 5%. The control is treated with purified water.

As shown in the photograph of FIG. 12, the SLS (1.0%) human patch test sold 1.0% SLS solution (w / v in distilled water) inside the upper arm of both subjects for the patch test. 20ul was soaked in a square chamber (1cm wide and 1cm long) of a patch (Haye's test chambers, USA) to cause irritation dermatitis and remove the patch artificially for 24 hours. 20ul of the sample was applied to the rectangular chamber of the patch at the same position where the patch was removed, and the sample was reattached for 24 hours. Then, the patch was removed and the skin reaction degree of the test site was evaluated after 30 minutes. , 63-71).

The evaluation results are presented in Table 8.

Criteria for dermal irritation efficacy evaluation criteria were based on the following criteria of the International Contact Dermatitis Research Group (ICDRG): Wooding et al, 1967; Rietschell, 1982; Fischer & Maibach, 1984; Aberer et al, 1993).

(Criteria)

0 = no redness

1 = erythema

2 = redness and papules

3 = redness, papules and vesicles

4 = severe edema and vesicles

The material used for the test was compared to the extract of silk grass, which is used as an irritant in cosmetics. No. 1 (Control) shown in FIG. 11 used water, No. 2 is a representative irritation-releasing substance, Machise extract, No. 3 is licorice extract, No. 4 is a Japanese cosmetic raw material used in cosmetics as irritation relaxation and anti-aging It is an anti-aging complex extract of Sasa and No. 5 is silk extract.

As a result, it was shown that the extract of silk grass has a skin irritant effect in the liver clinic of skin irritant alleviation.

Formulation

A lotion and cream were selected as the basic formulation of anti-aging cosmetics through hypoallergenic and feeling improvement.

Preparation Example 1 Lotion

Lotions were prepared with the compositions as listed in Table 9. In lotion preparation, emulsifiers, oils, antioxidants and preservatives are dissolved by heating at 80-82 ° C in an oil phase dissolving unit, and some additives and solvents are added to the manufacturing unit heated by dissolving at 78-80 ° C and a homomixer is added. After emulsification, the mixture was cooled to 40 ° C., the remaining additives were added, mixed stirring, and cooled.

The stability observation results of the prepared lotions are shown in Table 10 and the graphs of FIG. 13.

Preparation Example 2 Cream

Creams were prepared with the compositions as listed in Table 11. In cream production, emulsifiers, oils, antioxidants, and preservatives are melted by heating to 80 ~ 82 ℃ in the oil phase dissolving unit, and some additives and solvents are put into the dissolving unit at 78 ~ 80 ℃, and emulsified using HOMO MIXER. Cooling to 40 ° C., adding the remaining additives, mixing, stirring and cooling were used.

The stability observation results of the prepared cream are shown in Table 12 and FIG. 14.

Comparative experiment

Cell growth rate and antioxidant effect (DPPH assay) measurement results according to the concentration of the silk extract and the comparative substance according to the present invention are shown in Table 13 and Table 14, respectively.

Claims (12)

Euphorbia supina ) Wrinkle improvement cosmetic composition containing an extract as an active ingredient.
According to claim 1, wherein the silk ( Europhorbia) supina ) extract is a cosmetic composition for improving wrinkles, characterized in that the organic solvent extract.
The method of claim 2, wherein the organic solvent is improving wrinkles, characterized in that at least one member selected from the group consisting of C _{1 -4} lower alcohol, chlorinated hydrocarbon solvents, hydrocarbon solvents, aromatic hydrocarbon solvents and mixed solvents thereof Cosmetic composition for.
The cosmetic composition for improving wrinkles according to claim 2, wherein the organic solvent is at least one selected from the group consisting of ethanol, methanol, methylene chloride, chloroform, hexane, heptane, benzene, toluene, and a mixed solvent thereof.
According to claim 1, Euphorbia supina ) Wrinkle improvement cosmetic composition containing isoquercitrin (Isoquercitrin) isolated from the extract as an active ingredient.
Cosmetics containing the cosmetic composition of any one of Claims 1-5.
7. The cosmetic according to claim 6, wherein the cosmetic is from a group consisting of a lotion, an emulsion, a nourishing lotion, a nourishing cream, a massage lotion cream, a face wash cosmetic, a pack, a body product, an emulsified makeup, an ointment, a gel, a cream, a patch and a spray. Cosmetic product, characterized in that the selected formulation.
(a) pretreatment of Euphorbia supina with an organic solvent; And
(b) drying the residue is C _{1 -4} process for producing a lower alcohol bidanpul extract for improving wrinkles, comprising the step of extracting by using a solvent.
9. The method of claim 8, wherein the organic solvent of step (a) is a hydrocarbon solvent, and the solvent of step (b) is ethanol or methanol.
9. The method of claim 8, wherein the organic solvent of step (a) is hexane, and the solvent of step (b) is ethanol.
The method of claim 8, further comprising the step of (c) fractionating with a solvent after the step (b).
The solvent of claim 11, wherein the solvent is selected from the group consisting of hexane, methylene chloride, methyl acetate, ethyl acetate, purified water, methanol, ethanol, butanol, 1,3-butylene glycol, propylene glycol, glycerin, and benzene. Wrinkle improvement silk grass extract method characterized by the above solvent.

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