KR20210138017A - 핵산 분석 방법 - Google Patents

핵산 분석 방법 Download PDF

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Publication number
KR20210138017A
KR20210138017A KR1020217028674A KR20217028674A KR20210138017A KR 20210138017 A KR20210138017 A KR 20210138017A KR 1020217028674 A KR1020217028674 A KR 1020217028674A KR 20217028674 A KR20217028674 A KR 20217028674A KR 20210138017 A KR20210138017 A KR 20210138017A
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KR
South Korea
Prior art keywords
nucleic acid
acid molecule
primer
beads
sequence
Prior art date
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Ceased
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KR1020217028674A
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English (en)
Korean (ko)
Inventor
길라드 알모기
플로리안 오버스트라스
오머 바라드
찬단 쉬
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울티마 제노믹스, 인크.
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Publication of KR20210138017A publication Critical patent/KR20210138017A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020217028674A 2019-02-11 2020-02-10 핵산 분석 방법 Ceased KR20210138017A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962804082P 2019-02-11 2019-02-11
US62/804,082 2019-02-11
US201962890240P 2019-08-22 2019-08-22
US62/890,240 2019-08-22
US201962916683P 2019-10-17 2019-10-17
US62/916,683 2019-10-17
PCT/US2020/017491 WO2020167656A1 (en) 2019-02-11 2020-02-10 Methods for nucleic acid analysis

Publications (1)

Publication Number Publication Date
KR20210138017A true KR20210138017A (ko) 2021-11-18

Family

ID=72045127

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020217028674A Ceased KR20210138017A (ko) 2019-02-11 2020-02-10 핵산 분석 방법

Country Status (10)

Country Link
US (2) US12319959B2 (https=)
EP (1) EP3924515A4 (https=)
JP (1) JP7528103B2 (https=)
KR (1) KR20210138017A (https=)
CN (1) CN113728113A (https=)
AU (1) AU2020222888B2 (https=)
CA (1) CA3129315A1 (https=)
MX (1) MX2021009482A (https=)
SG (1) SG11202108563QA (https=)
WO (1) WO2020167656A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
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AU2020222888B2 (en) 2019-02-11 2024-08-01 Ultima Genomics, Inc. Methods for nucleic acid analysis
EP4200414A4 (en) * 2020-08-21 2024-10-09 Ultima Genomics, Inc. SURFACE AMPLIFICATION COMPOSITIONS AND USES THEREOF
AU2022328558A1 (en) * 2021-08-20 2024-04-04 Ultima Genomics, Inc. Systems and methods for sample preparation for sequencing
WO2023081883A2 (en) * 2021-11-08 2023-05-11 Ultima Genomics, Inc. Methylation sequencing methods and compositions
WO2025072163A1 (en) * 2023-09-25 2025-04-03 Ultima Genomics, Inc. Systems and methods for processing analytes

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WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
EP1641809B2 (en) * 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
JP2007526772A (ja) 2004-02-27 2007-09-20 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ インサイチュー配列決定用ポロニー蛍光ビーズ
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
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NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
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CN114854832A (zh) 2012-04-19 2022-08-05 生命技术公司 核酸扩增
EP2839029B1 (en) 2012-04-19 2017-11-15 Life Technologies Corporation Method of performing digital pcr
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CA3216609C (en) * 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
CN116622812A (zh) 2013-03-14 2023-08-22 生命技术公司 基质阵列和其制备方法
CN105189749B (zh) * 2013-03-15 2020-08-11 血统生物科学公司 用于标记和分析样品的方法和组合物
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SG11201803646YA (en) * 2015-11-04 2018-05-30 Atreca Inc Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
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Also Published As

Publication number Publication date
AU2020222888A1 (en) 2021-09-30
MX2021009482A (es) 2021-11-12
EP3924515A4 (en) 2022-08-24
JP7528103B2 (ja) 2024-08-05
US12319959B2 (en) 2025-06-03
SG11202108563QA (en) 2021-09-29
US20260078438A1 (en) 2026-03-19
AU2020222888B2 (en) 2024-08-01
EP3924515A1 (en) 2021-12-22
WO2020167656A1 (en) 2020-08-20
CN113728113A (zh) 2021-11-30
CA3129315A1 (en) 2020-08-20
JP2022519324A (ja) 2022-03-22
US20220042072A1 (en) 2022-02-10

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